secondary messenger
TRANSCRIPT
-
8/9/2019 Secondary Messenger
1/50
SecondaryMessenger SystemFlorlin Grace M. Yrad
-
8/9/2019 Secondary Messenger
2/50
http://www.rsc.org/chemistryworld/sites/default/ les/upload/Endocrine-
Endocrine
System
-
8/9/2019 Secondary Messenger
3/50
O R M O N E
-
8/9/2019 Secondary Messenger
4/50
Did you know that not
all hormones canpass through the
phospholipid bilayer?
-
8/9/2019 Secondary Messenger
5/50
$on-polar hormones cannot
penetrate the cellmem%rane.
http://www.per&inelmer.com/'MS(esources/)mages/**-
-
8/9/2019 Secondary Messenger
6/50
Non-steroid hormon
Receptor Protein
http://courses.washington.edu/con#/gprotein/trimeri
-
8/9/2019 Secondary Messenger
7/50
*
G-protein Coupled Receptor(GPCR) or G-protein Linked
Receptor
* It contain seven membrane-spanning
regions with their N-terminalsegment on the exoplasmic face and
their C-terminal segment on the
cytosolic face of the plasmamembrane.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC !"#"$"/
-
8/9/2019 Secondary Messenger
8/50
* ut wait! " lot o#scientist ha$e been
proposing to drop theterm GPCR in #a$or o#
% transmembrane orhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC !"#"$"/
-
8/9/2019 Secondary Messenger
9/50
G-protein 0in&ed (eceptor
http://www.nc%i.nlm.nih.go1/%oo&s/$23 +4+,/ gure/5 44 /6
-
8/9/2019 Secondary Messenger
10/50
-
8/9/2019 Secondary Messenger
11/50
-
8/9/2019 Secondary Messenger
12/50
G-protein has ! su%-units:
alpha8 %eta and gamma.
http://pu%s.acs.org/su%scri%e/archi1e/mdd/1"4/i++/html/ gures/++"*feature
-
8/9/2019 Secondary Messenger
13/50
G-protein has ! su%-units:
alpha8 %eta and gamma.
http://pu%s.acs.org/su%scri%e/archi1e/mdd/1"4/i++/html/ gures/++"*feature
-
8/9/2019 Secondary Messenger
14/50
G-protein 0in&ed (eceptor
http://www.nc%i.nlm.nih.go1/%oo&s/$23 +4+,/ gure/5 44 /6
-
8/9/2019 Secondary Messenger
15/50
-
8/9/2019 Secondary Messenger
16/50
* econdary 'essengers
*
'hey relay informationfrom the receptor-
ligand complex.
http://www.ncbi.nlm.nih.gov/boo(s/N)*$ $+,/
-
8/9/2019 Secondary Messenger
17/50
E9ects of SecondaryMessengers
Secondary messengers are free to di9use tothe di9erent compartments of the cell whichallows them to in uence gene e;pression andother processes.
Secondary messengers may greatly amplifysignals.
)nput from signalling pathways or crosstal&may e9ect the concentrations of secondarymessengers. )t may result in nely tunedregulation or misinterpretation.
http://www.nc%i.nlm.nih.go1/%oo&s/$23 + " /
-
8/9/2019 Secondary Messenger
18/50
-
8/9/2019 Secondary Messenger
19/50
*
Cyclic "'P or c"'P
* It is formed from 'P byadenylyl cyclase and
degraded by the en yme
c MP Phosphodiesterase.
http://www.ncbi.nlm.nih.gov/boo(s/N)* !"+/
-
8/9/2019 Secondary Messenger
20/50
-
8/9/2019 Secondary Messenger
21/50
-
8/9/2019 Secondary Messenger
22/50
http://courses.washington.edu/con#/%ess/gpcr/adenylate-cyclase.png
-
8/9/2019 Secondary Messenger
23/50
-
8/9/2019 Secondary Messenger
24/50
2inding of c5M=to the regulatorysu%units inducesa conformational
change that leads
to dissociation ofthe catalytic
su%units8 which
are thenenBymaticallyacti1e.
http://www.nc%i.nlm.nih.go1/%oo&s/$23@,4"/ gure/5 !4/67
-
8/9/2019 Secondary Messenger
25/50
http://www.nc%i.nlm.nih.go1/%oo&s/$23@,4"/ gure/5 !,/6
-
8/9/2019 Secondary Messenger
26/50
Ahe free
catalyticsu%unit of
protein&inase 5translocates
to thenucleus.
http://www.nc%i.nlm.nih.go1/%oo&s/$23@,4"/ gure/5 !@/6report7o%#ectonly
-
8/9/2019 Secondary Messenger
27/50
)tphosphorylates
thetranscriptionfactor '(E2
-
8/9/2019 Secondary Messenger
28/50
c5M= can also regulate ion channelsthat are independent of proteinphosphorylation.
http://www.rci.rutgers.edu/CuBwia&/5nat=hys/'hemicalSomaticSenses les/image"+ .#pghttp://www.nc%i.nlm.nih.go1/%oo&s/$23@,4"/
http://www.rci.rutgers.edu/CuBwia&/5nat=hys/'hemicalSomaticSenses.htm
-
8/9/2019 Secondary Messenger
29/50
-
8/9/2019 Secondary Messenger
30/50
*
Cyclic G'P or cG'P
* It is formed from 0'P bythe en yme g%anylyl
cyclase and degraded by
the en ymephospodiesterase.
http://%sers.rcn.com/&(imball.ma.%ltranet/)iologyPages/1/1econd2messengers.htmlhttp://www.ncbi.nlm.nih.gov/boo(s/N)* !"+/
-
8/9/2019 Secondary Messenger
31/50
-
8/9/2019 Secondary Messenger
32/50
+: (eceptor 2ound Guanylyl'yclase
-
8/9/2019 Secondary Messenger
33/50
: $itric D;ide
http://www.eplasty.com/article images/eplasty+"e* g+.gif
-
8/9/2019 Secondary Messenger
34/50
-
8/9/2019 Secondary Messenger
35/50
-
8/9/2019 Secondary Messenger
36/50
*
*nositol trisphosphate (*P +) orinositol-, . /-triphosphate
* It is released into the cytoplasmwhere it releases calci%m ions
from internal stores within the
cell3s endoplasmic retic%l%m.
http://p%bchem.ncbi.nlm.nih.gov/s%mmary/s%mmary.cgi4cid567 6,#
-
8/9/2019 Secondary Messenger
37/50
*
Diacylglycerol 9D"G
* It activates protein-
serine/threonine (inases belongingto the protein (inase C family; manyof which play important roles in the
control of cell growth anddifferentiation.
http://www.ncbi.nlm.nih.gov/boo(s/N)* !"+/
-
8/9/2019 Secondary Messenger
38/50
Phosphatidy#inosito# $%&-
'isphosphate (P)P * +
https://www.caymanchem.com/images/catalog/
http://cn;.org/resources/ @d4fd4%+* * " ,@d,*!,"f 4+,e,4/Figur
-
8/9/2019 Secondary Messenger
39/50
p ; g @ ,@ , , , , ge "@ " "*.#pg
-
8/9/2019 Secondary Messenger
40/50
http://legacy.owens%oro.&ctcs.edu/gcaplan/anat /notes/5=)) "$otes+ "endocrin"intro.htm
-
8/9/2019 Secondary Messenger
41/50
http://%io%oo&.nerin;hs.org/%%/cells/cell %iology/+"""p;-
-
8/9/2019 Secondary Messenger
42/50
http://%io%oo&.nerin;hs.org/%%/cells/cell %iology/+"""p;-
-
8/9/2019 Secondary Messenger
43/50
ydrolysis of =)= %y =hospholipase '
http://www.nc%i.nlm.nih.go1/%oo&s/$23@,4"/ gure/5 **/6
http://cn;.org/resources/ @d4fd4%+* * " ,@d,*!,"f 4+,e,4/Figur
-
8/9/2019 Secondary Messenger
44/50
e "@ " "*.#pg
-
8/9/2019 Secondary Messenger
45/50
-
8/9/2019 Secondary Messenger
46/50
?5
G
-
8/9/2019 Secondary Messenger
47/50
'almodulin
-
8/9/2019 Secondary Messenger
48/50
http://mol-%iol*masters.masters.gr&ra#.org/html/'ellular Signal Aransduction*-
-
8/9/2019 Secondary Messenger
49/50
http://cgmp.%lauplanet.com/reg/%yp&g.htmlhttp://courses.washington.edu/con#/gprotein/trimericgp.htm
http://cgmp.%lauplanet.com/path.htmlhttp://www .estrellamountain.edu/faculty/fara%ee/%io%&/%io%oo&endocr.htmlhttp://users.rcn.com/#&im%all.ma.ultranet/2iol
ogy=ages/S/Second messengers.htmlhttp://www.nc%i.nlm.nih.go1/%oo&s/$23 + " /http://www.nc%i.nlm.nih.go1/pmc/articles/=M'+,4 4 4/
http://www.nc%i.nlm.nih.go1/%oo&s/$23 +4+,/
S
-
8/9/2019 Secondary Messenger
50/50
http://www.nc%i.nlm.nih.go1/%oo&s/$23@,4"/http://www.mun.ca/%iology/desmid/%rian/2)D0" "/2)D0 " "-+*/'2+*.html
http://legacy.owens%oro.&ctcs.edu/gcaplan/anat /notes/5=)) "$otes+ "endocrine"intro.htm
S