serum biomarkers for lung cancer

Serum Biomarkers for Lung CancerResults and Conclusion of the Master Thesis

Fellowship Work in IndiaInternship at Goethe Universitätsklinikum Frankfurt

SERUM BIOMARKERS FOR LUNG CANCER &Other Project Works

Bharati Singh

Frankfurt am MainJune 3, 2013

Presentation atINSTITUT FÜR PHARMAZIE UND BIOCHEMIE,

JOHANNES GUTENBERG UNIVERSITÄT, MAINZ

Bharati Singh Cancer Research



Outline

1 Serum Biomarkers for Lung CancerMotivation for ThesisWhat is Biomarker?Objective of the Master ThesisMethods Used & Experimental steps

2 Results and Conclusion of the Master ThesisResultGraph of Biochemical ParametersConclusion & Future Work

3 Fellowship Work in IndiaFellowship Work

4 Internship at Goethe Universitätsklinikum FrankfurtInternship Project and Methods




Motivation for ThesisWhat is Biomarker?Objective of the Master ThesisMethods Used & Experimental steps

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MOTIVATION

Detection of Lung Cancer at Early Stage—

Most patients with lung cancers are diagnosed at inoperableadvanced stages and prognosis is particularly dismal,with the five year survival rate for stage 3b and stage 4diseases being 5 precent and 1 percent respectively.





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WHAT IS BIOMARKER

Biomarkers:By NATIONAL CANCER INSTITUTE: Biomarker is abiological molecule found in blood, other body fluids ortissue that is a sign of a normal or abnormal process orof a condition or disease.By CENTER FOR BIOMARKERS IN IMAGING: Biomarkers areAnatomic, Physiologic, biochemical or molecularparameters associated with the presence and severityof specific disease states.By BIOMARKERS CONSORTIUM: Biomarkers arecharacteristics that are objectively measured andevaluated as indicators of normal biological processes,pathogene processes or pharmacology responses totherapeutic intervention.

Example of Biomarkers: CARCINOEMBRYONIC ANTIGEN,CYFRA21-1, TISSUE POLYPEPTIDE ANTIGEN





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OBJECTIVE OF THE THESIS

1 To develop Lung Cancer model in Wistar rat.2 To study protein changes in serum by 1 Dimensional and 2

Dimensional gel electrophoresis.3 Study of stress related enzymes.





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METHODS USED

NNK treatmentsSerum collection and analysisProtein Estimation Using Folin Lowry methodSDS PAGESilver StainingBiochemical parameters





EXPERIMENTAL STEPS - NNK TREATMENT

Figure: Experimental steps performed for “NNK Treatment”





EXP. STEPS - SERUM COLLECTION AND ANALYSIS

Figure: Exp. steps performed for “Serum Collection and Analysis”Bharati Singh Cancer Research



ResultGraph of Biochemical ParametersConclusion & Future Work

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ESTIMATION OF PROTIEN CONCENTRATION

Figure: Total protein estimation in mg/ml at different time intervals at2.5mg/kg b.wt. of NNK in treated male wistar rats





IMAGE OF SDS PAGE

Figure: Protein profile of serum sample for control and treated. (withNNK) on male wistar rats on 12% SDS-PAGE gel. (4th week)





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LIPID PEROXIDATION

Figure: Activity of enzyme (mM/ ml/min) at different time intervals at2.5mg/kg b.wt. of NNK in treated male wistar rats.





REDUCED GLUTATHIONE (GSH)

Figure: Activity of Reduced Glutathione (GSH) (mM/ml/min) atdifferent time intervals at 2.5mg/kg b.wt. of NNK in treated malewistar rats.





GRAPH OF BIOCHEMICAL PARAMETERS

Figure: Activity of Catalase (mM/ml/min) at different time intervals at2.5mg/kg b.wt. of NNK in treated male wistar rats.





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CONCLUSION & FUTURE WORK

Conclusion:Changes analyzed from the one dimensional and twodimensional protein profile were insignificant.No significant results obtained while observing changesat protein level.

Future Work:Convert an entire protein mixture to peptides.Alternative technique: Isotope Coded Affinity Tags(ICAT)




Fellowship Work

Outline








Fellowship Work

FELLOWSHIP WORK IN INDIA

Topic - STUDIES ON IMMUNOTOXIC PROFILE AND PROTEOMICS

OF DELTAMETHRIN IN MURINE THYMOCYTES AND

SPLENOCYTES: ATTENUATION BY HERBALS

Work and Methods used:Literature SurveyMTT assayELISA




Internship Project and Methods

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INTERNSHIP PROJECT AND METHODS

Topic - DISTRIBUTION OF CD31 EXPRESSING ENDOTHELIAL

CELLS IN DIFFERENT HCC MODEL

Various methods and technologies usage:ImmunohistochemistryConfocal MicroscopyPCRMRIImageJ





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serum biomarkers for lung cancer

Health & Medicine

murine thymocytes

cd31 expressing

signicant

goethe universittsklinikum

time intervals

goethe universittsklinikum

abnormal process

objectively