skin equivalent through bioprinting · skin equivalent through bioprinting léa pourchet 1, amélie...
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Skin equivalent through bioprinting
Léa POURCHET1, Amélie THEPOT2, Morgan DOS SANTOS3, Aurélie BOHER2, Loïc BLUM1, Christophe A. MARQUETTE1, *
1. Institute of Chemistry and Molecular and Supramolecular Biochemistry Team Enzyme Engineering, Biomimetic Membranes and Supramolecular Assemblies, CNRS 5246 ICBMS, Université Lyon 1, Villeurbanne, France. 2. LabSkin Creations, Edouard Herriot Hospital, 5 place d'Arsonval, Bâtiment 5, 69437, Lyon, France.3.Cell and Tissue Bank, Cutaneous Substitute Laboratory, Edouard Herriot Hospital, 5, place d'Arsonval, Pavillon I, 69437, Lyon, France.
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The Lyon Biochip group:-We have an open source and application driven approach.-We aim at filling the gap between technology and application using additive technology.
The 3d.FAB platform: the only French platform dedicated to academic and private innovation through 3D printing, in the field of health.
The printed dermis project = made dermis via bioprinting
Introduction
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A. Platform
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Platform presentation
3D FAB = 3D Fabric of Advanced Biology
Platform is specialized in biology/material interaction.
2 areas of expertise : - Biochemistry for diagnostic.- Biology for regenerative medicine.
http://fabric-advanced-biology.univ-lyon1.fr/
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Available technology
Biochemistry, especially diagnosis Regenerative medicine
Soft materials for biology : the future of medicine, via bio-extrusion and photopolymerization with a large range of hydrogel like polymers.
Hardened materials for rapid prototyping of diagnostic devices with multicomponent possibilities.
TOBECA® Stratasys
TOBECA® B9 Creator
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B. Bioprinting process
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Bio-extrusion process
Biomaterials mix Cells Bio-ink
Bioprinter
Final object
Cell maturation step
Loading Bio-ink into a syringe
Patent number : 15634617
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Bio-ink
The special bio-ink formulation has three purposes: - maintaining an appropriate gel rheology during the extrusion process, - enabling the consolidation of the printed object during the post-processing step- allowing the adequate development of the 3D cell network leading to a correct organisation and function of the maturate tissue.
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Bioprinter
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Examples
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Maturation step
In this step, we need to control :- Degradation rate of the hydrogel which is replaced by neosynthetised ECM - Addition of growth factor
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Allows cell proliferation and synthesis of Extra-Cellular Matrix
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C. Results of bioprinting dermis
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Macroscopic appearance
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Printed dermis morphology D12
Harris' hematoxylin–phloxin–saffron (HPS) staining :
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Control without cells
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Printed dermis morphology D13
X10 X40
Vimentin staining allows to see cytoskeleton in green and nucleus in blue
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Printed dermis morphology D30
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Keratinocytes seeding at D5 Keratinocytes seeding at D12
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Printed dermis morphology D38
Masson’s Trichrome staining :
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Harris' hematoxylin–phloxin–saffron (HPS) staining :
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Epidermis marker : Fluorescent microscopy observation
Cytokeratin 10 Ki 67 Laminin 332Filaggrine
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Hu
man
skin
Skin
eq
uiv
alen
tP
rin
ted
skin
Epidermal differentiation and dermal marker profile of bio-printed SEs in comparison to NHS from healthy donor. Scale bar: 50 µm.
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Dermal MarkerH
um
ansk
inSk
in e
qu
ival
ent
Pri
nte
dsk
in
Elastine Vimentin Fibrilin Collagen I Collagen V
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Conclusion
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Skin printing implantable immediately
Skin printing directlyon the patient
Bio-inkCells sample
What can we do : - Skin printing- Cartilage printing too
Next step : printed skin for burned people
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Thanks for your attention
Contact : [email protected]
Website : http://fabric-advanced-biology.univ-lyon1.fr/