Slide PreparationSlide Preparation

D. Crowley, 2007D. Crowley, 2007

L/O - To be able to prepare a microscope slide

L/O - To be able to prepare a microscope slide

Microscope CrosswordMicroscope Crossword

Have a go at the microscope crossword - you have five minutes…

Down: -

1. Lens that you look down

2. What the slide is placed on

3. A wheel to make the image clear

Across: -

4. Lens closest to the slide

5. The specimen is placed on this

6. You need this to see something

Have a go at the microscope crossword - you have five minutes…

Down: -

1. Lens that you look down

2. What the slide is placed on

3. A wheel to make the image clear

Across: -

4. Lens closest to the slide

5. The specimen is placed on this

6. You need this to see something

EyepieceStageFocusing

ObjectiveSlideLight

MicroscopesMicroscopes Set up a microscope to view an animal cell - remember the steps to take

Place the smallest objective lens (the smallest lens) over the hole in the stage

Turn the coarse focusing wheel to make the gap between the stage and the objective lens as small as possible

Place a slide on the stage, and secure with the clips Adjust the light source so light goes up through the stage Turn the coarse focusing wheel slowing, until your

specimen is in focus To see your specimen in more detail, turn the next largest

objective lens over your specimen Use the fine focusing wheel to get your image into focus

again - do not use the coarse focusing wheel - this can break your slide!

Set up a microscope to view an animal cell - remember the steps to take

Place the smallest objective lens (the smallest lens) over the hole in the stage

Turn the coarse focusing wheel to make the gap between the stage and the objective lens as small as possible

Place a slide on the stage, and secure with the clips Adjust the light source so light goes up through the stage Turn the coarse focusing wheel slowing, until your

specimen is in focus To see your specimen in more detail, turn the next largest

objective lens over your specimen Use the fine focusing wheel to get your image into focus

again - do not use the coarse focusing wheel - this can break your slide!

Preparing SpecimensPreparing Specimens A specimen is the object we look at under a microscope

The specimen needs to be thin, so light can pass through it

We use a coverslip between the slide and specimen which helps flatten it out. It also helps keep the specimen in place, and stops it drying out

When you have prepared your specimen you can look at it under the microscope. It is vital you know what magnification you are using: this is easy to work out - it is the eyepiece lens x the objective lens:

Magnification = magnification eyepiece lens x magnification objective lens

A specimen is the object we look at under a microscope

The specimen needs to be thin, so light can pass through it

We use a coverslip between the slide and specimen which helps flatten it out. It also helps keep the specimen in place, and stops it drying out

When you have prepared your specimen you can look at it under the microscope. It is vital you know what magnification you are using: this is easy to work out - it is the eyepiece lens x the objective lens:

Magnification = magnification eyepiece lens x magnification objective lens

Preparing OnionsPreparing Onions You are going to prepare a slide containing a specimen of onion cells

Follow the slide preparation steps, then draw what you see (using A4 plain paper and in pencil). Also write down your magnification

Magnification = magnification eyepiece lens x magnification objective lens

You are going to prepare a slide containing a specimen of onion cells

Follow the slide preparation steps, then draw what you see (using A4 plain paper and in pencil). Also write down your magnification

Magnification = magnification eyepiece lens x magnification objective lens

Place a drop of water on the centre of a slide

(this may contain a stain to make the

specimen more clear, e.g. iodine)

Cut some onion, then using

forceps, peel off the inside layer

of a piece of onion

Place your onion skin onto the

water droplet on your slide

Using a mounted needle, lower a

coverslip onto the onion skin. Do this

slowly and carefully, to avoid

trapping air bubbles!

MagnificationMagnification

When you have prepared your specimen you can look at it under the microscope. It is vital you know what magnification you are using: this is easy to work out - it is the eyepiece lens x the objective lens:

Magnification = magnification eyepiece lens x magnification objective lens

E.g. eyepiece lens = 10xobjective lens = 60x Magnification = 10 x 60

= 600x magnification

When you have prepared your specimen you can look at it under the microscope. It is vital you know what magnification you are using: this is easy to work out - it is the eyepiece lens x the objective lens:

Magnification = magnification eyepiece lens x magnification objective lens

E.g. eyepiece lens = 10xobjective lens = 60x Magnification = 10 x 60

= 600x magnification

Marking your drawingsMarking your drawings Level 3

Prepare a slide with help Focus the image with help Indentify parts of the microscope Draw what they can see Identify parts of cell with help

Level 4 Prepare a slide with help Focus the image with help Identify most parts of microscope and state their job Draw simple diagram of cell seen under microscope Identify nucleus and cell wall

Level 5 Prepare slide safely, with only a little help Focus microscope with only a little help Name most parts of microscope and describe how

they work Draw an accurate diagram of cells under the

microscope Identify and label parts of cell correctly

Level 3 Prepare a slide with help Focus the image with help Indentify parts of the microscope Draw what they can see Identify parts of cell with help

Level 4 Prepare a slide with help Focus the image with help Identify most parts of microscope and state their job Draw simple diagram of cell seen under microscope Identify nucleus and cell wall

Level 5 Prepare slide safely, with only a little help Focus microscope with only a little help Name most parts of microscope and describe how

they work Draw an accurate diagram of cells under the

microscope Identify and label parts of cell correctly

Marking your drawingsMarking your drawings Level 5

Prepare slide safely, with only a little help Focus microscope with only a little help Name most parts of microscope and describe how they

work Draw an accurate diagram of cells under the microscope Identify and label parts of cell correctly

Level 6 Prepare a slide safely without help Focus the image at low and high magnification Identify all parts of microscope and describe how they

work Draw an accurate diagram of cell seen under microscope Identify and label parts of cell correctly Make a reasonable estimate of the size of the cell

Level 7 Prepare a slide safely and competently without help Focus the image at all 3 magnifications Explain in detail how the microscope works Draw an accurate diagram of cell seen under microscope Identify and label parts of cell correctly, identifying

problems Make a accurate measurement of the size of the cell

Level 5 Prepare slide safely, with only a little help Focus microscope with only a little help Name most parts of microscope and describe how they

work Draw an accurate diagram of cells under the microscope Identify and label parts of cell correctly

Level 6 Prepare a slide safely without help Focus the image at low and high magnification Identify all parts of microscope and describe how they

work Draw an accurate diagram of cell seen under microscope Identify and label parts of cell correctly Make a reasonable estimate of the size of the cell

Level 7 Prepare a slide safely and competently without help Focus the image at all 3 magnifications Explain in detail how the microscope works Draw an accurate diagram of cell seen under microscope Identify and label parts of cell correctly, identifying

problems Make a accurate measurement of the size of the cell