smart strips - tecna rapid tests for mycotoxins
TRANSCRIPT
The rapid and cost effective screening solution for multiple mycotoxins screening
Mycotoxins co-occurrence
Almost 70% of the cereals in the world present mycotoxin contamination
In 30% of the cases the contamination levels are well above the law limits
With all these premises, mycotoxins co-occurrence is a widely diffused phenomenon
Different toxic fungi proliferation can be favoured by similar climate conditions
° C 15 20 25 30 35
ZON, T2
DON
DON
FUMONISINS
AFLATOXINS
Aspergillus flavusAspergillus parasiticus
Fusarium moniliformeFusarium verticilloides
Fusarium proliferatum
Fusarium graminearum
Mycotoxins are widely diffused toxic metabolites of different fungi species
Mycotoxins toxicity main targets are:
- Liver- Kidneys- Immune system- Nervous system- Endocryne system- Mucosa- Reprpductive
system
The effects of mycotoxins contamination on human and animal health can be predictable in case of single mycotoxin occurrence. However, if there are several occurring mycotoxins, the relative synergistics effects are barely known and can be unpredictable.
Mycotoxins co-occurrence
Noteworthy, in case of multiple mycotoxins contamination, adverse effects might occur even if the single mycotoxins levels are lower than regulatory ones.
The field
Theharvest
Thestocking
The transit
The acceptance
check
The processing
There are some critical points when SPEED and EASE of USE of screening tests are fundamental.
Possibility of parallel screening is the key factor to rapidly determine mycotoxin co-occurrence and to asses products safety.
Mycotoxins not only develop in the field; they proliferate along the whole agro-food chain as well.
Screening along the agro-food chain
SPEED and EASE of USE of a test not necessarily enable to avoid the risks of mycotoxins co-occurrence.
Deoxynivalenol
Aflatoxins
Zearalenone
Fumonisins
Single screeningneeds
- 4 exctracts to prepare- 4 reading sessions- 4 time consumables usage
Parallel screeningneeds
- Single exctract to prepare- One reading session- Use consumables just
once
Parallel screening provides unravelled time and money save on mycotoxins screening.
Tecna Smart Strips for mycotoxins enable parallel screening for the main mycotoxins as aflatoxins, deoxynivalenol, zearalenone and fumonisins.
Parallel screening not only provides resources save but also reduces error probability as less steps are performed.
Single screening vs parallel screening
Tecna Smart Strip kits share the same sample preparation and assay procedure:
1. Weight the ground cereal2. Add the extracting solution3. Shake 3 minute4. Filter5. Dilute
6. Mark the cassette 7. Add the extract on the strip8. Wait 5 or 10 minutes – incubation at ROOM TEMPERATURE
9. Read
Tecna Smart Strip - general features
Tecna Smart Strip – steps are illustrated in the Smart Chart
Every kit contains an illustrated Smart Chart to support analysts in performing the assay
The LAB LFD READER allows to analyze up to 5 strips per time, even for different analytes. The provided result is either qualitative or quantitative.
Key features of the LAB LFD reader are:• little space required• PC connession through USB wire• read out of max 5 strips per time – different tests also• software for results analysis• software to archive results
Tecna LAB FLD READER for digital analysis of results
MAIZE
WHEAT
Samplepreparation
DURUM WHEAT
1:3 in 70% methanol extraction, shake for 3 minutes, filter or centrifuge, dilute
1:5 in water extraction,
shake for 3', centrifuge,
dilute
WHEAT
Tecna Smart Strip - technical features
2 – 75 ppbUp to 10 – 375
ppb
Detection range
50 – 1000 ppbUp to 100 – 2000
ppb
150 – 4000 ppbUp to 750 – 20000
ppb
LOQ 5 ppb 75 ppb 250 ppb 500 ppb
Assay time 10 min 10 min5 min
10 ppb 100 ppb
Assay time 5 min
250 -12500 ppb
Applicability
Cut off
Quantitative analysis
Qualitative analysis
Aflatoxin B1 and zearalenone Smart Strips allow a qualitative pre-reading of the result within specific cut offs.
Quantitative results are obtained for aflatoxin B1, zearalenone, fumonisins and deoxynivalenol.
Batch specific barcode is the easy way to set the LAB LFD READER for the ananlysis for one or more mycotoxins simultaneosly.I.e: - analysis for one mycotoxin = 1 barcode
- analysis for three mycotoxins = 3 barcodes
Tecna Smart Strip - technical features
The plastic house of the Smart Strip can be used for labelling thereof, thus favouring sample tracking during multiple screening for mycotoxins.
Smart Strip AFLA B1
20 det. – cod. MA820
Smart Strip ZON 20 det. – cod. MZ800
Smart Strip FUMO 20 det. – cod. MF800
Smart Strip DON 20 det. – cod. MD800
For more information on the rapid test line dedicated to mycotoxins screening
www.tecnalab.com
For further information on mycotoxins look for the Tecna Mycotoxins Cards in all ELISA and lateral flow kits or directly book your own deck. Tecna
Mycotoxins Cards: learning about mycotoxins has never been that easy!
Tecna Smart Strip - the product lineup