smetana lab - centrum pro přenos poznatků a technologií · smetana k jr, gál p, kolár m....
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OFFER
■ Cell cultivation. ■ Detection of molecules using histochemical methods. ■ Functional evaluation of cells. ■ Tissue engineering.
EXPERTISE
■ Managing advanced tissue cultures. ■ We are dedicated to the separation and cultivation of stem
cells from human epidermis. ■ Developing methods of characterization and diff erentiation of
the obtained cell types. ■ We investigate the infl uence of microenvironment on the func-
tion of stem cells and their diff erentiation during healing.
Prof. Karel Smetana, M.D., DrSc.Biotechnology and Biomedicine Center of the Academy of Sciences and Charles University in Vestec – BIOCEVPrůmyslová 595252 50 Vestec+420 325 873 [email protected]
SMETANA LAB
„We study stem cells in epidermis and their application in cell therapy.“
CURRENT PROJECTS
■ Microenvironment of malignant melanoma as a factor of tumor aggressiveness. GAČR 16-05534S.
■ Tumor microenvironment of head and neck carcinoma: Pro-gnostic signifi cance of extracellular matrix produced by tumo-rassociated fi broblasts. AZV 15-28933A.
■ Cellular and molecular characteristics of neonatal human skin: consequences for skin healing. GAČR 13-20293S.
PARTNERS
■ General Faculty Hospital. ■ Prague Institute of Molecular Genetics of the ASCR, v.v.i,
Prague.
MOST SIGNIFICANT RECENT PUBLICATIONS AND PATENTS
■ Jobe, N.P., Rösel, D., Dvořánková, B., Kodet, O., Lacina, L., Mateu, R., Smetana, K. Jr., Brábek J. Simultaneous block ing of IL-6 and IL-8 is suff icient to fully inhibit CAF-induced human melanoma cell invasiveness. Histochem Cell Biol. 146: 205–217, 2016.
■ Jobe, N.P., Živicová, V., Mifková, A., Rösel, D., Dvořánková, B., Kodet, O., Strnad, H., Kolář, M., Šedo, A., Smetana, K. Jr., Strnadová, K., Brábek, J., Lacina, L.“ Fibroblasts potentiate melanoma cells in vitro invasiveness induced by UV-irradiated keratinocytes. Histochem Cell Biol. doi: 10.1007/s00418-018-1650-4.
■ Solís, D., Bovin, N.V., Davis, A.P., Jiménez-Barbero, J., Romero, A., Roy, R., Smetana, K. Jr., Gabius, H.-J.: A guide into glyco-sciences: How chemistry, biochemistry and biology cooperate to crack the sugar code. Biochim. Biophys Acta. 1850:186–235, 2015.
■ Szabo, P., Kolář, M., Dvořánková, B., Lacina, L., Štork, J., Vlček, Č., Strnad, H., Tvrdek, M., Smetana, K., Jr.: Mouse 3T3 fi bro-blasts under the infl uence of fi broblasts isolated from stroma of human basal cell carcinoma acquire properties of multipo-tent stem cells. Biol. Cell 103:233–248, 2011.
■ Valach J, Fík Z, Strnad H, Chovanec M, Plzák J, Cada Z, Szabó P, Šáchová J, Hroudová M, Urbanová M, Šteffl M, Pačes J, Mazánek J, Vlcek C, Betka J, Kaltner H, André S, Gabius H-J, Kodet R, Smetana K Jr, Gál P, Kolár M. Smooth muscle actin-expressing stromal fi broblasts in head and neck squamous cell carcinoma: increased expression of galectin-1 and induction of poor-pro-gnosis factors. Int J Cancer 131: 2499–2508, 2012.