smooth muscle cell biology, expression profiling, and histological analyses thoracic aortic...
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![Page 1: Smooth Muscle Cell Biology, Expression Profiling, and Histological Analyses Thoracic Aortic Aneurysms Stefan Blunder Cardiac Surgery Research Laboratory](https://reader035.vdocument.in/reader035/viewer/2022081512/5697bfa21a28abf838c963ec/html5/thumbnails/1.jpg)
Smooth Muscle Cell Biology, Expression Profiling,
and Histological Analyses
Thoracic Aortic Aneurysms
Stefan Blunder
Cardiac Surgery Research Laboratory Medical University of Vienna
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Insight into cell biological processes involved in the formation of thoracic aortic aneurysm.
“wh
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issu
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smooth muscle cells
Thoracic aortic aneurysms.
Goal of the study.
www.lab.anhb.uwa.edu.au
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Thoracic aortic aneurysms…
Project Plan.
2. Development of a protocol for the isolation of primary SMC cell lines from human TAAs.
3. Assessment of basic cellular functions of primary SMCs.
4. Establishment of a broad gene expression profile of primary SMCs.
1. Histological analyses of human TAA specimens.
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TAVBAVHistological Analysis.
SMC actin & nuclei & elastic fibres.
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Histological Analysis.
Vimentin & Marson Trichrome.
Marson
Trichrome.
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Smooth muscle cells – basic cellular characteristics.
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Smooth Muscle Cells.
Gene expression profiling.
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Smooth Muscle Cells.
Osteopontin.
*
AOsteopontin
*
BTissue inhibitor of metalloproteinase 3
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Smooth muscle cells.
Osteopontin.
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Smooth muscle cells.
TIMP 3.
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Thoracic aortic aneurysms.
Conclusions.
* … p<0.05%; n.d. … not determined
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Thoracic aortic aneurysms.
Conclusions.
• Changes in aortic SMCs may crucially contribute to TAA initation and formation.
• SMCs from aneurysm show signs of premature aging.
• BAV and TAV TAAs have distinct pathomechanisms.