soy bean using iplant resources. how we started iplant workshop –cshl
TRANSCRIPT
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Soy bean using iplant resources
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How we started
• iPlant workshop –CSHL
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Introduction
• 14 different samples – Seeds • 5, 6 and 7 stages well watered and drought stress
– Different parts of Roots• And 2 time points 72 and 96 hours as well well watered
and drought stress
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Analysis-Quality checks
• We performed quality checks using FASTX toolkit – Locally as well as in DE
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Analysis
• Tuxedo suite of tools• Bowtie for the alignment of the filtered data• Cufflinks suite for the analysis• We are checking the needs of the project so
that we can add more functionalities into our pipelines
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Quality Analysis
SWS-R5 SWS-R6 SWS-R7 SWW-R5 SWW-R6 SWW-R7
Seeds
96-LRS-R2
Lateral Root
96-LRS-R1 96-LRC-R2 96-LRC-R1 72-LRS-R2 72-LRS-R1 72-LRC-R2 72-LRC-R1
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GC contentSWS-R5 SWS-R6 SWS-R7 SWW-R5 SWW-R6 SWW-R7
Seeds
96-LRS-R2
Lateral Root
96-LRS-R1 96-LRC-R2 96-LRC-R1 72-LRS-R2 72-LRS-R1 72-LRC-R2 72-LRC-R1