special stains on artisan link pro€¦ · slides on instrument, staining combination and staining...

Atlas of Stains

Special Stains on Artisan Link Pro

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Intended useRoutinely processed samples (paraffin-embedded) may be used. The preferred fixative is neutral buffered formalin. The clinical interpretation of any staining, or its absence, should be complemented by morphological studies and proper controls, and should be evaluated within the context of the patient’s clinical history and other diagnostic tests by a qualified pathologist. All reagents have been optimally prepared for use on the Artisan Staining System and require no mixing or diluting.

StorageEach component should be stored at the temperature indicated on the label. Do not use after expiration date. If reagents are stored under any conditions other than those specified, the conditions must be verified by the user. There are no obvious signs to indicate instability of this product. Therefore, appropriate tissue must be run as controls for such verification. If unexpected staining is observed which cannot be explained by variations in laboratory procedures or improper storage, contact Agilent Technical Support.

Time per Slide*Actual Time per Slide varies depending on a number of factors, including but not limited to: placement on carousel, quantity of slides on instrument, staining combination and staining protocol. Using onboard drying and deparaffinization will add additional time to the throughput.

Note: This document is a general overview. For specific information on individual products, please consult that specific instruction for use.

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AR162 Acid-Fast Bacteria (AFB) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4AR362 Acid-Fast Bacteria (AFB) Light Green. . . . . . . . . . . . . . . . . . . . . 6AR169 Alcian Blue/PAS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 AR169 + AR171 Alcian Blue/PAS with Alpha-Amylase . . . . . . . . . . . . . . . . . . . 10AR178 Alcian Blue/PAS/Hematoxylin . . . . . . . . . . . . . . . . . . . . . . . . . 12AR160 Alcian Blue pH 2.5. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14AR307 Colloidal Iron . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16AR161 Congo Red . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18AR163 Elastic . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 AR164 Giemsa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22AR167 Gomori’s Blue Trichrome . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24 AR166 Gomori’s Green Trichrome . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 AR175 Gram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28AR306 Gram Yellow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30AR176 Grocott’s Methenamine Silver (GMS) . . . . . . . . . . . . . . . . . . . 32AR376 Grocott’s Methenamine Silver (GMS) Eosin . . . . . . . . . . . . . . 34 AR158 Iron . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36AR180 Jones’ Basement Membrane (PAS-M) . . . . . . . . . . . . . . . . . . 40AR480 Jones’ Basement Membrane (PAS-M) H&E . . . . . . . . . . . . . . 42 AR380 Jones’ Basement Membrane (PAS-M) Light Green . . . . . . . 44AR173 Masson’s Trichrome . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46AR168 Mucicarmine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48AR313 Orcein . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50AR165 Periodic Acid-Schiff (PAS) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52AR165 + AR171 Periodic Acid-Schiff (PAS) with Alpha-Amylase . . . . . . . . . . . 54AR172 Periodic Acid-Schiff (PAS) Green. . . . . . . . . . . . . . . . . . . . . . . . 56AR182 Reticulin/No Counterstain . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 AR179 Reticulin/Nuclear Fast Red . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60 AR181 Warthin-Starry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

Index

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Acid-Fast Bacteria Varying shades of red

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Intended for laboratory use to aid the identification, by light microscopy, of acid-fast bacteria, such as Mycobacterium, in tissue samples.

Acid-Fast Bacteria (AFB)

Product Code AR162

Staining Interpretation: –– Acid-fast bacteria: Varying shades of red–– Background: Light blue

Section Thickness : 4 μm

Control Tissue: Tissue infected with acid-fast bacteria

Time per Slide*: 0:12:43

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Acid-Fast Bacteria Red

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Intended for laboratory use to aid the identification, by light microscopy, of acid-fast bacteria, such as Mycobacterium, in tissue samples.

Acid-Fast Bacteria (AFB) Light Green

Product Code AR362

Staining Interpretation: –– Acid-fast bacteria: Varying shades of red–– Background: Light green


Control Tissue: Tissue infected with acid-fast bacteria


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Acidic Mucins Blue

Glycogen

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Intended for laboratory use to aid the identification, by light microscopy, of acidic and neutral mucins in tissue samples.

Alcian Blue/PAS

Product Code AR169

Staining Interpretation: –– Neutral mucins: Magenta–– Acidic mucins: Blue–– Mixtures of acidic and neutral mucins: Color

depends on the dominant entity and will range from blue-purple or purple through violet or mauve


Control Tissue:

–– Neutral mucin: Stomach, prostate–– Acidic mucin: Skin, umbilical cord–– Weakly sulfated epithelial mucin: Large

intestine


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Acidic Mucins Blue

Digested Glycogen

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Intended for laboratory use to aid the identification, by light microscopy, of acidic and neutral mucins in tissue samples. Alpha-amylase digestion removes any glycogen in the tissue.

Alcian Blue/PAS with Alpha-Amylase

Product Code AR169 (with AR171)

Staining Interpretation: –– Neutral mucins: Magenta–– Acidic mucins: Blue–– Mixtures of acidic and neutral mucins:

Color depends on the dominant entity and will range from blue-purple to violet-mauve


Control Tissue: Stomach, colon or umbilical cord


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Nuclei Black to blue

Neutral Mucins Magenta

Acidic Mucins Blue

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Alcian Blue/PAS/Hematoxylin

Product Code AR178

Staining Interpretation: –– Neutral mucins: Magenta–– Acidic mucins: Blue–– –Mixtures of acidic and neutral mucins:

Color depends on the dominant entity and will range from green or purple through violet or mauve

–– Nuclei: Black to blue


Control Tissue:

–– Neutral mucin: Stomach, prostate–– Acidic mucin: Skin, umbilical cord–– Weakly sulfated epithelial mucin: Large

intestine


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Cytoplasm: Pale pink

Weakly sulfated acidic mucopolysaccharides,

sulfomucins, hyaluronic acid, sialomucins:

Dark blue

Nuclei: Red to pink

15


Alcian Blue pH 2.5

Product Code AR160

Staining Interpretation: –– Weakly sulfated acidic mucopolysaccharides, sulfomucins, hyaluronic acid, sialomucins: Dark blue

–– Nuclei: Red to pink–– Cytoplasm: Pale pink


Control Tissue: Small intestine, umbilical cord or colon


16

Cytoplasm: Light pink

Nuclei: Light pink

Carboxylated and Sulfated Mucopolysaccharides and Glycoprotein Mucin: Dark blue

17

Colloidal IronIntended for laboratory use to aid the identification, by light microscopy, of carboxylated and sulfated mucopolysaccharides and glycoprotein mucin in tissue samples.

Product Code AR307

Staining Interpretation: –– Carboxylated and sulfated mucopolysaccharides and glycoprotein mucin: Dark blue

–– Nuclei: Light pink–– Cytoplasm: Light pink


Control Tissue: Small intestine


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Amyloid: Apple green

Nuclei: Blue

Amyloid, collagen, other fibrous materials: Pale pink/salmon

19

Intended for laboratory use to aid the identification, by polarized light microscopy, of amyloid in tissue samples.

Congo Red

Product Code AR161

Staining Interpretation: Polarized Light Microscopy–– Amyloid: Apple green

Light Microscopy–– Amyloid, collagen, other fibrous materials:

Pale Pink/salmon–– Nuclei: Blue


Control Tissue: Tissue with amyloid or heart tissue


20

Elastic Fibers: Black

Nuclei: Blue to black

Collagen: Red

21

Intended for laboratory use to aid the identification, by light microscopy, of elastic fibers in tissue samples.

Elastic

Product Code AR163

Staining Interpretation: –– Elastin fibers: Black–– Nuclei: Blue to black–– Collagen: Red–– Other tissue elements: Yellow


Control Tissue: Cross section of artery, appendix and skin


22

Lymphocytes: Blue

Eosinophils: Bright pink

23

Lymphocytes: Blue

Intended for laboratory use to aid the identification, by light microscopy, of hematopoietic cells (i.e. mast cells, basophils, polymorpho-nuclear leukocytes etc), in tissue samples.

Giemsa

Product Code AR164

Staining Interpretation: –– Mast cell granules: Purple–– Basophils: Purple–– Eosinophils: Bright pink–– Lymphocytes: Blue


Control Tissue: Tissue containing mast cells


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Nuclei: Blue or black

Collagen: Blue

Cytoplasm: Red

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Intended for laboratory use to aid the identification, by light microscopy, of connective tissue in tissue samples.

Gomori’s Blue Trichrome

Product Code AR167

Staining Interpretation: –– Cytoplasm: Red–– Fibrin: Pink–– Collagen: Blue–– Nuclei: Blue or black–– Erythrocytes: Red


Control Tissue: Uterus, kidney and liver


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Collagen: Green

Nuclei: Blue or black

Cytoplasm: Red

Fibrin: Pink

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Intended for laboratory use to aid the identification, by light microscopy, of connective tissue in tissue samples.

Gomori’s Green Trichrome

Product Code AR166

Staining Interpretation: –– Cytoplasm: Red–– Fibrin: Pink–– Collagen: Green–– Nuclei: Blue or black–– Erythrocytes: Red


Control Tissue: Uterus, small intestine, liver, appendix and fallopian tube.


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Gram-positive Bacteria: Blue

Gram-negative Bacteria: Red

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Intended for laboratory use to aid the identification, by light microscopy, of Gram-positive and Gram-negative microorganisms in tissue samples.

Gram

Product Code AR175

Staining Interpretation: –– Gram-positive bacteria: Blue–– Gram-negative bacteria: Red–– Background: Varying shades of blue/green


Control Tissue: Tissue containing both Gram-positive and Gram- negative bacteria.


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Gram-positive Bacteria: Dark blue

Gram-negative Bacteria: Light pink to magenta

31

Gram YellowIntended for laboratory use to aid the identification, by light microscopy, of Gram-positive and Gram-negative microorganisms in tissue samples.

Product Code AR306

Staining Interpretation: –– Gram-positive bacteria: Dark blue–– Gram-negative bacteria: Light pink to magenta–– Background: Yellow


Control Tissue: Tissue containing both Gram-positive and Gram-negative bacteria.


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Pneumocystis Jirovecii: Black

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Intended for laboratory use to aid the identification, by light microscopy, of fungal organisms and Pneumocystis jirovecii (formerly known as carinii) in tissue samples.

Grocott’s Methenamine Silver (GMS)

Product Code AR176

Staining Interpretation: –– Fungi: Black–– Pneumocystis: Black–– Background: Light green


Control Tissue: Tissue with Aspergillus, Candida or Pneumocystis


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Fungi: Black

35

Intended for laboratory use to aid the identification, by light microscopy, of fungal organisms and Pneumocystis jirovecii (formerly known as carinii) in tissue samples.

Grocott’s Methenamine Silver (GMS) Eosin

Product Code AR376

Staining Interpretation: –– Fungi: Black–– Pneumocystis: Black–– Background: Pink


Control Tissue: Tissue with Aspergillus, Candida or Pneumocystis


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Ferric Iron: Blue

Nuclei: Red

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Intended for laboratory use to aid the identification, by light microscopy, of ferric iron deposits in tissue samples.

Iron

Product Code AR158

Staining Interpretation: –– Ferric iron: Blue–– Nuclei: Red–– Background: Pink


Control Tissue: Spleen or liver with hemosiderosis


38

Basement Membrane: Black

Nuclei: Red

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Intended for laboratory use to aid the identification, by light microscopy, of basement membranes in tissue samples.

Jones’ Basement Membrane (PAS-M)

Product Code AR180

Staining Interpretation: –– Basement membrane: Black–– Nuclei: Red–– Background: Pink


Control Tissue: Kidney with glomeruli


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Nuclei: Blue

41

Jones’ Basement Membrane (PAS-M) H&E Intended for laboratory use to aid the identification, by light microscopy, of basement membranes in tissue samples.

Product Code AR480

Staining Interpretation: –– Basement membrane: Black–– Nuclei: Blue –– Background: Pink




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43

Jones’ Basement Membrane (PAS-M) Light Green Intended for laboratory use to aid the identification, by light microscopy, of basement membranes in tissue samples cut at 2 μm.

Product Code AR380

Staining Interpretation: –– Basement membrane: Black–– Background: Light green




44

Muscle: Red

Collagen: Blue

45

Masson’s TrichromeIntended for laboratory use to aid the identification, by light microscopy, of connective tissue and muscle in tissue samples.

Product Code AR173

Staining Interpretation: –– Muscle fibers: Red–– Fibrin: Pink–– Collagen: Blue–– Nuclei: Blue or black–– Erythrocytes: Red


Control Tissue: Uterus, small intestine, liver, appendix or fallopian tube


46

Nuclei: Black

Mucin: Pink

47

Intended for laboratory use to aid the identification, by light microscopy, of epithelial mucins in tissue samples.

Mucicarmine

Product Code AR168

Staining Interpretation: –– Mucin: Pink–– Nuclei: Black–– Background: Yellow


Control Tissue: Small intestine, colon or appendix


48

HBsAG: Dark reddish brown

Copper: Dark red-purple

Elastin Fibers: Dark reddish brown

49

OrceinIntended for laboratory use to aid the identification, by light microscopy, of the viral inclusion bodies of hepatitis B surface antigen (HBsAG) and Copper-associated proteins in tissue samples.

Product Code AR313

Staining Interpretation: –– HBsAG/elastin fibers: Dark reddish brown–– Copper-associated protein: Dark red-purple–– Background: Pale pink to pink


Control Tissue:

Liver tissue containing positive HBsAG. Fetal liver can be used for copper or liver with Wilson’s disease. A multi-block containing positive liver for HBsAG and copper with normal liver is recommended.


50

PAS-positive structures: Magenta

Nuclei: Blue

51

Intended for laboratory use to aid the identification, by light microscopy, of glycogen and muco-polysaccharide components in tissue samples.

Periodic Acid-Schiff (PAS)

Product Code AR165

Staining Interpretation: –– PAS-positive structures: Magenta–– Nuclei: Blue–– Background: Pink


Control Tissue: –– Kidney for basement membrane–– Liver with glycogen


52

Nuclei: Blue

53

Intended for laboratory use to aid the identification, by light microscopy, of glycogen and mucopoly-saccharide components in tissue samples. Alpha-amylase digestion removes any glycogen in the tissue.

Periodic Acid-Schiff (PAS) with Alpha-Amylase

Product Code AR165 (with AR171)

Staining Interpretation: –– Nuclei: Blue–– Background: Pink


Control Tissue: Liver containing glycogen


54

Fungi: Magenta

55

Intended for laboratory use to aid the identification, by light microscopy, of fungi in tissue samples.

Periodic Acid-Schiff (PAS) Green

Product Code AR172

Staining Interpretation: –– Fungi: Magenta–– Background: Blue - green


Control Tissue: Skin tissue with Candida albicans


56

Reticulin Fibers: Black

57

Intended for laboratory use to aid the identification, by light microscopy, of reticulin fibers in tissue samples.

Reticulin/No Counterstain

Product Code AR182

Staining Interpretation: –– Reticulin fibers: Black


Control Tissue: Liver, spleen and lymph node


58

Reticulin Fibers: Black

59

Intended for laboratory use to aid the identification, by light microscopy, of reticulin fibers in tissue samples.

Reticulin/Nuclear Fast Red

Product Code AR179

Staining Interpretation: –– Reticulin fibers: Black–– Background: Red


Control Tissue: Liver, spleen or lymph node


60

Helicobacter Pylori: Black

Spirochetes: Black

61

Intended for laboratory use to aid the identification, by light microscopy, of Helicobacter pylori and spirochete microorganisms in tissue samples.

Warthin-Starry

Product Code AR181

Staining Interpretation: –– Helicobactor pylori: Black–– Spirochetes: Black–– Background: Golden yellow


Control Tissue: Tissue with helicobacter and spirochetes


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Artisan Link Pro SolutionThe consistent, safe and easy choice for special stains.

– Designed for multi staining of up to 14 different stains simultaneously

– 48 slide capacity for optimal productivity and overnight run Fully automated with on-board drying and deparaffinization capabilities

– 27 high-quality ready-to-use automated stain kits

– 29 optimized, validated protocols that are editable for easy yet flexible results

– Proven consistent staining quality

– Waste separation into four separate streams

– DakoLink Software with LIS and LAN/WAN connectivity enables full lab integration

– Complete data and staining transparency enabling optimized performance and full traceability

– Dedicated Service & Support provides fast, professional support when hands-on assistance is needed

For additional information on Artisan Link Pro or other Dako solutions, contact your local Agilent representative or visit www.agilent.com.

This information is subject to change without notice.

© Agilent Technologies, Inc. 2018 Published in the USA, June 4, 2018 29030 2018JUN04

special stains on artisan link pro€¦ · slides on instrument, staining combination and staining...

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