sperm hyliter karl reich, ph.d. independent forensics lombard il a new and better forensic test for...
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SPERM
HY LITER
Karl Reich, Ph.D.Independent Forensics
Lombard IL
A New and Better Forensic Test for Human Blood
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BLOOD
15 min
adsorb stain onto swabextract in providedextraction buffer
RSIDTM
Running Buffer
mix extract aliquotwith running buffer
(100 l total)
add to RSID-Blood
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HY LITER
BLOOD
15 min
adsorb stain onto swabextract in providedextraction buffer
RSIDTM
Running Buffer
mix extract aliquotwith running buffer
(100 l total)
add to RSID-Blood
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HY LITER
BLOOD
Sensitivity Testing
0 l 1 l@1:5 1 l 5 l 100 lExtract volume
Equiv. vol human blood
0 l 10 nl 250 nl 1 l 5 l
20 l
50 nl
50 l of humanblood extracted
in 1 ml BEB
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BLOOD
Total Extract Volume (l) 0 1,000 500 200
Equiv. volumehuman blood (l)
0 5 10 25
No High Dose Hook Effect
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BLOOD
Testing RSID-BLOODTM for Species Specificity
20 l of Extract1 l equivalent
neg – negative controlpos – positive control F – ferret C – bonobo chimpanzee G – gorilla
neg pos F C G
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BLOOD
neg pos
ferret
1 l 1 l 1 l20 l 20 l 20 l
gorilla orangutan
RSID-BLOODTM
Species Specificity: Direct Comparison
neg H F G O
Hexagon OBTI
Seratec Hemedirect
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BLOOD
RSID™-BLOOD: Competitive Analysis
Technology Specificity Sensitivity Ease of Running Method Use Time
RSID™-BLOOD Strip Test High Medium Easy 10 min
(antibody) CONFIRMATORY‡ NO HD HOOK*
Hemoglobin-Based Strip Test Low to High Med 10 min Tests (antibody) Medium HD HOOK Effect
Chemical-Based Heme Poor Low Easy 10 min Tests Chemistry Home Brew Antibody Low Low Difficult 10 min ‡ The Only CONFIRMATORY TEST for human blood – No animal or primate cross reactivity -
*No High Dose Hook Effect
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BLOOD
Specificity Testing
Animal Species Tested:ferret, orangutan, gorilla, lowland gorilla, baboon, spider monkey, skunk, bonobo chimpanzee, goat, sheep, pig, horse, cat, mouse, dog, bull, Box turtle, opossum, American Elk, mule deer, alpaca, Siberian tiger, Nubian goat,
NO CROSS REACTIONS IDENTIFIED- INCLUDING PRIMATES –
CONFIRMATORY FOR HUMAN BLOOD
Body Fluids Tested:sweat, semen, urine, saliva, vaginal secretions, double mixtures, triple mixtures
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Questions
Comments Samples Gripes
Karl Reich
Tel 708.234.1200Fax 708.978.5115
www.IFI-test.com