Stem Cell Therapy in DogsA New Treatment for a New Decade

Presented by Vet-Stem

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What are Stem Cells?Stem Cells are primitive cells present in almost every tissue

• Self Renewing

• Able to become different tissue types

• Signal other cells to come in and rescue and repair

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What Do Adult Stem Cells Do?

10 million cells die in your body every minute of

every day.

Your stem cells replace them so you can continue living.

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Embryonic vs Adult Stem Cells

Embryonic Stem Cells

• Intended to form whole animal - not for repair• Can form tumors – UNPREDICTABLE• Ethical dilemma• Maybe someday

Adult Stem Cells – many sources

• Intended for self repair• No ethical dilemma• Do not form tumors when injected• Available now

Gruen L and Grabel L, Stem Cell 2006;24;2162-2169.

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Why Fat as Stem Cell Source?

• High healing cell count – No culturing

required

• 1000 times more stem cells than bone

marrow

• Family of healing cells

• Rapid, Easy to access

• Over 1700 peer reviewed papers published

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What Do Stem Cells Do?

• Reduce Inflammation

• Reduce pain

• Differentiation into tissue

• Home in on injury sites

• Induce healing-regeneration

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The Injury Response Cascade

Time

Fibrosis

Inflammation

“Regeneration”

Acute InjuryAcute Injury Scar Complete

Ma

gn

itu

de

QUICK QUICK FIXFIX

Courtesy of Dr.Caplan, Case Western Reserve

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“Regeneration”

Acute InjuryAcute Injury Scar Reduced

Time

Ma

gn

itu

de

Inflammation

Fibrosis

Stem Cells in Injury Response

Courtesy of Dr. Caplan, Case Western Reserve

Stem CellsStem Cells

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Annie

Annie 9 yr LabX

Chronic elbow arthritis

Drugs not controlling pain

Stem Cell Therapy

Able to jump in and out of car; took 2 hour run in the woods 90 days after stem cell therapy

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Harley 6 yr Rat terrier

Pain in hips & knees since 6 months old

Pain killer medications (NSAIDS) for 12 months & still painful

Wouldn’t play ball or go up stairs

2 weeks after stem cells– Dramatic pain reduction

Plays ball and climbs stairs

Now NSAID free for >36 months

Harley

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Blinded Clinical Trial Participant

Cinnamon 10 yr Standard Poodle

Arthritis in both hips

Unable/unwilling to take medications

Owners considering euthanasia-got a new puppy

30 days after stem cell therapy he was playing, jumping, running and taking long walks

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Got Fat?

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Why Use Vet-Stem?

• Began treating horses in 2003

• Experienced-Over 3,500 horses & 2,000

dogs

• Safety-your pet’s own cells

• Strict quality standards

• Peer-reviewed, published controlled studies

• Global leader in veterinary stem cell therapy

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Horses:7/3,359 (0.2%) Only local swelling/pain

Dog: 2/1,695 (0.1%) Only local swelling/pain

*probable adverse events, through 6/1/09

Safety Profile*

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• Written standard operating procedures

• Independent Quality Assurance Unit

• Routine audits of all procedures

• Sample release criteria

• Formal training systems

Quality Standards

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Service Overview

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Vet-Stem Adipose Processing Lab

1. Immediate use

3. Lifetime supply by culture

2. Frozen for future

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Current Supported UsesDogs/Cats

• Osteoarthritis• Polyarthritis• Tendonitis• Ligament injury

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Pet w/ lameness or pain due to arthritis or a partial tear of a ligament or tendon

Pet does not require surgery – surgery first, if needed

No other major diseases (liver, kidney, heart)

No major spinal disease (spondylosis, spinal arthritis)

Not-responsive or can’t tolerate pain killers

Realistic expectations

Ideal Stem Cell Case

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Cruciate Ligament Disease & Stem Cells

• Surgery – always have surgery when required

• With surgery – helps to reduce inflammation and scar tissue

• After surgery – helps to treat inflammation and encourage cartilage repair

• Chronic ACL inflammation and synovitis is a good case

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Storm

Storm 9yr Catahoula Leopard Dog Mix

Arthritis in both hips and right stifle

Unable/unwilling to take medications

3 weeks after treatment started showing improvement

Later his left stifle was treated after surgical repair

Storm and owners happy that he is doing extremely well

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Real World Dog OA Cases

24

Average Veterinary Scores of Dogs in Regards to Pain, Lameness, and Range of Motion

2008 Commercial Clinical Data*

1.0

1.5

2.0

2.5

3.0

3.5

Pain(N=141, pre-tx)

Lameness(N=146, pre-tx)

Range Of Motion(N=141, pre-tx)

Ve

teri

na

ry S

co

re

Veterinary Scale:5=Extreme (considering euthanasia)4=Severe3=Moderate2=Mild1=Normal

*Voluntary responses from commercial cases.

Day 0 30 60 90 Day 0 30 60 90 Day 0 30 60 90

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Conclusion: According to a 2009 Owner Survey

• Greater than 33% of dogs discontinued the use of NSAIDs completely

• Greater than 28% of dogs decreased their dependency on NSAIDs

At 90 and 246 days after treatment

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Why Vet-Stem is the Only Choice for

Quality and Experience

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• Never collect fat again. Grow more cells as needed. Some clinics have to collect fat repeatedly.

• Precise cell counts. Some clinics inject unknown numbers of cells.

• Excellent safety record with thousands of dogs.

• Controlled lab for stem cell processing instead of processing in the clinic in the presence of hair and other potential contaminants.

• Vet-Stem implements FDA guidelines to ensure quality. Some in-clinic kits are labeled “kit to be used for research”.

Malibu

Before and After Video

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Media Coverage of Veterinary Medicine

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Questions?

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