stem cell therpay a hope to no hope disoders
TRANSCRIPT
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Dr. Sharda Jain Dr. Ajay
saxena Dr. Rajesh
Dhall
:A Hope to No Hope Disorders
Stem Cell Therapy
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Life is too Precious - don’t give up !!
Mother Teresa
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STEM CELL Therapy
A HOPE TO NO HOPE DISORDERS
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The fascinating new world of STEM CELL THERAPY : a medical revolution at the corner
Welcometo
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Future of Stem Cell Medicine
• The Indian market potential slated to be in the range of US$ 500
million.
• More than 45,000 people across the world receiving stem cell
transplant every year.
• Potential to reduce pharmaceutical R&D by 25% each year,
with savings of up to US $25 million in each drug market.
• Potential to be the next revolution in the field of medicine.
LifeCare – ReeCure Centreof STEM CELL Therapy
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“The Need”
• The physical misery
• Psychological trauma
Of “No Hope Disorder” is great !!
• No cure available for most of these diseases
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Rationale of is stem cell Therapy ??
Introduction of adult stem cells into damaged tissue in order to treat
injuries / Disease in
“No Hope Disorders”
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Stem cell potential… beating cardiac muscle
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• Is it really working?• Why not studies are coming from premier institutes of
country ?• Is it really possible to segregate Mesenchymal cells?• Differentiation of cells out side body possible in India???• If it is too good then, why not NOBEL PRIZE given ?• ?• ??• ???• Is this therapy available in India?
Questions ????
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Times of
India
9th October 2012
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We are sure there are a lot of doubts plaguingyour mind about STEM CELL BANKING and
STEM CELL THERAPY.
Let us help you to ERASE these doubts.
Doubts… on SCT
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1913 the great Spanish neuroscientist Santiago Ramón y Cajal pronounced “that in adult Neurone & the nerve paths –
Everything may die, nothing may be regenerated”.
Nerve Cell Cannot
Regenerate ?
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Heart Brain
Same is our perception for any tissue !!
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Beliefs turned out to be FALSE as several types of cells can regenerate
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Major Milestones Which Triggered Adult Stem Cell
Research ?
• Since1970’s, BONE MARROW TRANSPLANTS
have been used for treatment of Immunodeficient &
leukemia (with Good results).
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2nd Breakthrough…
History of Human Embryonic Stem Cell Research
In 1998, James Thomson (University of Wisconsin-Madison) isolated cells from the inner cell mass of the blastocyst, and developed the first human embryonic stem cell line in culture.
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Prediction was made of Making cells and replacing all the diseased cells?
& even gametes
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Stem Cell Potential
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Stem Cell History • 1970 – BM transplant for leukaemia • 1998 - Researchers first extract stem cells from human embryos • 1999 - First Successful human transplant of insulin-making cells from cadavers • 2001 - President Bush restricts federal funding for embryonic stem-cell research • 2002 - Juvenile Diabetes Research Foundation International creates $20 million fund-raising effort to support stem- cell research • 2003?? - California ok stem cell research • 2004 - Harvard researchers grow stem cells from embryos using private funding • 2004 - Ballot measure for $3 Billion bond for stem cells• 2006-7 – Private Banking for cord & cord blood in india. • 2014 – Public cord banking Kolkatta – Inst. Tropical med.
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What are Stem Cells
• Ability to differentiate
• Ability to regenerate
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Stem Cell is a •young,
•primitive and unspecialized cell with a remarkable potential to renew,
•Differentiate and Develop into any desired tissue or organ of the body.
What is stem cells ?
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Self renewal: Unlimited proliferative potential.
Differentiation: Differentiate into various cell types Pluripotency, Multipotency, Unipotency)
Plasticity : Trans-differentiation
Regeneration potential: a means of Repair
Characteristic Features
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A single precursor
What is Differentiation?
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Cells having infinite self renewing capacity and potential for differentiation.
What are Stem Cells?
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1000 IEC ICAs in biocompatible capsule IP Tx
STZ Diabetic mice
Blood glucose analysis of STZ induced diabetic mice transplanted with hUCMSCs
and Islet like clusters (ILC)
0 10 20 300
100
200
300
400
500
hUCMSCsILCsNormalDiabetic control
Days after transplantationB
loo
d g
luc
os
e c
on
ce
ntr
ati
on
mg
/dl
0 25 50 75 100 125 1500
100
200
300
400ILCdiabetic controlnon diabetic control
time (mins)
Blo
od
glu
co
se c
on
cen
trati
on
mg
/dlGlucose
Tolerance Test
Fasting blood glucose concentration of diabetic mice after transplantation of ILCs showed that the hyperglycemic mice reverted back to normoglycemia. Intraperitoneal glucose tolerance test was performed on 45th day, post-transplantation. Mice transplanted with ILCs showed similar blood glucose profile as compared to the normal non diabetic controls.
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How MSCs work In vivo
Under normal circumstances MSC contribute to tissue maintenance, contributing to immune homeostasis by avoiding unnecessary immune cell response.
With damaged tissue, harmonious interaction between MSC and endothelial cells are disrupted, allowing blood extravasations , blood clot form, platelet release multitude of bioactive molecule, immune cells are attracted where they start immune surveillance and immune response.
MSC secrete bioactive molecules that exert anti-apoptotic, immunomodulatory , angiogenic , anti-scaring , anti-fibrotic and chemoattractant effects
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Source of Adult Stem cells • Autologus, • Allogeneic or • Xenogenic
Fetal tissue is the best current tissue source for human stem cells,
However ethical issues are a major concern. (ICMR – Autologous)
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The embryos of
aborted fetuses
Umbilical cord
and cord blood of a
new born baby
Bone Marrow
Peripheral
Bloodstream
Adult Tissues Amniotic Fluid
MENSTRUAL BLOOD Dental Pulp
Sources of Stem Cells
Hair
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Best Source
Amniotic fluid, umbilical cord, Cord blood - Rich Source of Stem Cells
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Cord Blood Stem Cells• Can be used only
to cure chronic blood-related disorders such as sickle cell disease, Thalasemia, and leukaemia.
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Umbilical Cord Blood Stem Cell Transplant
• Umbilical cord blood stem cell transplants are less prone to rejection than either bone marrow or peripheral blood stem cells. This is probably because the cells have not yet developed the features that can be recognized and attacked by the recipient's immune system
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Placental Blood as a Source of Hematopoietic Stem Cells for Transplantation into
UNRELATED RECIPIENTS
Joanne Kurtzberg, M.D.et al
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PLASTICITY: The ability of one tissue to produce cells of other developmentally unrelated tissues
Traditionally, stem cells were believed to be Lineage Restricted and Organ Specific.
Bone marrow stem cells: The gold standard
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AUTOLOGUS – STEM CELLS
• While most blood stem cells reside in the bone marrow, a small number are present in the bloodstream. These multipotent peripheral blood stem cells, or PBSCs, can be used just like bone marrow stem cells to treat leukaemia, other cancers and various blood disorders
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Adult Stem Cells - Hair
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Dental Pulp
Menstrual Blood
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Allogeneic – Stem Cells • Sources of stem cells
from another donor (allogeneic) are primarily relatives (familial- allogeneic) or completely unrelated donors (unrelated-allogeneic). The stem cells in this situation are extracted from either the donor's body or cord blood
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Xenogeny - Stem Cells • In this stem cells from
different species are transplanted, e.g. porcine fetal ventral mesencephalic (FVM) xenotransplants for Parkinson's disease.
This has no major ethical concerns and a large amount of tissue is available, however life long immunosupression and risk of rejection are the major limitations
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Prediction for Future Cells from these Animal will
be used in human beingsWithout risk of rejection
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THE SUCCESS OF STEM CELL THERAPY DEPENDS ON LAB.
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These are • Mono Nuclear cells ( MNC )
• Hematopoietic Stem Cells [HSC] family: CD34+; CD45; CD133
• Mesenchymel count in M.N.C is just 0.001%• Mesenchymel stem cell [MSC]: CD73; CD90;
CD106 Etc ..........
Classification Of Stem Cells
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Differentiation with different cluster coding/desire cells type ( as per disorder) through culture.. (Mesenchymal types are - CD 73, CD 90, CD 105 etc . .. . .
• Multiplication up label of required cells quantity.( near to 1.5 Cr.).
• Composition of all cells like Mnc, Hsc & Msc.
Technology Behind Success
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Bone Marrow Menstrual Blood Hair Follicle Adipose Tissue
STEM CELL THERAPY
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Homogeneous population and high rate
of cell division
Homogeneous population and high rate
of cell division
No risk of rejection –
USED across HLA barrier
No risk of rejection –
USED across HLA barrier
No ethical issues
No ethical issues
Immune privilege
d
Immune privilege
d
Greater potency of
cultured expanded
product
Greater potency of
cultured expanded
product
Precise identification
Precise identification
Ease of isolation and scale up
Ease of isolation and scale up
Genetic stabilityGenetic stability
Advantages Advantages of of
Mesenchymal Mesenchymal
Stem CellsStem Cells
No risk of teratoma formation
No risk of teratoma formation
PluripotentPluripotent
Efficient large scale expansion
Efficient large scale expansion
Lower cost of cell culture
process
Lower cost of cell culture
process
Why MSCs ?
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Indications
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Indications
STEM CELL Therapy
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• Diabetes
• Cerebral Palsy
• Multiple Sclerosis
• Parkinson’s Disease
•Alzheimer’s Disease
• Autism
• Anti-Ageing
Very Effective
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Diabetes
STEM CELL THERAPY FOR DIABETES
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Neuro-MuscularDisorders
• Cerebral Palsy √
• Multiple Sclerosis √
• Parkinson’s Disease √• Autism √
• Spinal Cord Injury
• Stroke
•Alzheimer’s Disease
• Muscular Dystrophy
• Spinal Muscular Atrophy
• ALS
STEM CELL Therapy for
Neuro – Muscular Disorders
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STEM CELL THERAPY FOR
Anti-Ageing
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Effective
• Liver Cirrhosis• Non-healing fractures
• Stroke • Osteogenesis Imperfecta
• Muscular Dystrophy • Myocardial Infarction
• Spinal Muscular Atrophy • Cardiomyopathy
• ALS • Chronic Heart Failure
• Osteoarthritis • Osteogenesis Imperfecta
• Non-healing fractures
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• Osteoarthritis
• Non-healing fractures
• Osteogenesis Imperfecta
• Achondrodysplasia
Bone and Cartilage Disorders
Cardiovascular Disorders
• Chronic Heart Failure• Myocardial Infarction• Cardiomyopathy• Buerger’s Disease
STEM CELL Therapy for Bone and Cartilage Disorders
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KIDNEY LIVER
STEM CELL THERAPY FORKIDNEY /LIVER DISORDER
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Least Effective
• Spinal Cord Injury
• Buerger’s Disease
• Eye Disorder
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Not Effective/ Need More Trails
Acute Leukaemia • Inherited Platelet Abnormalities
• Chronic Leukaemia • Other Inherited Disorder
• Myelodysplastic Syndromes • Inherited Metabolic Disorders
• Marrow Failure • Histiocytic Disorders
• Thalassemia • Inherited Erythrocyte Abnormalities
• Myeloproliferative Disorders • Inherited Immune System Disorders
• Lymphoproliferative Disorders • Plasma Cell Disorders
• Phagocyte Disorders • Other Malignancies
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Drug Testing - Quicker
• Stem cells could allow scientists to test new drugs using human cell line which could speed up new drug development.
• Only drugs that were safe and had beneficial effects in cell line testing would graduate to whole animal or human testing.
• It would allow quicker and safer development of new drugs.
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Let’s Create a better tomorrow
for
Spread the message
Please create Public Awareness
Spinal injury My cord blood treated
Bhai’s thalassemia major Cured of insulin dep. DM
Super man
Ajit Jogi
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Only company in India with 9 type of stem cell banking and treatment through Reecure
50 centres spread across India
Mr. Ajay SaxenaPh. 8467888005Website : www.reelabs.com Email : [email protected]
Q. & A. session Dr. Sharda Jain
Mr. Ajay Saxena
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STEM CELL Therapy
A HOPE TO NO HOPE DISORDERS
Thank you