Stereo Zoom Light Microscopy - ZEISS Axio Zoom.V16

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Contents Brief Description ............................................................................................................................ 3

Reference ...................................................................................................................................... 3

Location ......................................................................................................................................... 3

Suggestion for “Materials and Methods” ..................................................................................... 3

Suggestion for “Acknowledgements” ........................................................................................... 4

People and Contacts...................................................................................................................... 4

Optics ............................................................................................................................................ 4

Features and Specifications .......................................................................................................... 5

Objective ....................................................................................................................................... 5

Filter-Sets ...................................................................................................................................... 6

Additional Equipment ................................................................................................................... 6

System Overview ........................................................................................................................... 7

WARNINGS and Advice ................................................................................................................. 7

Potential Techniques ..................................................................................................................... 8

Set Up ............................................................................................................................................ 9

Sample Preparation ................................................................................................................... 9

Initial Set Up .............................................................................................................................. 9

Final Set Up ............................................................................................................................. 12

ZEN 2.1 (blue edition) Guidelines ................................................................................................ 13

Single Image Acquisition or Visualization................................................................................ 14

Multidimensional Image Acquisition (Multi-Channel Images) ................................................ 18

Channel Combine or Image Overlay ....................................................................................... 26

Extracting Single Fluorescence Images of a Multichannel Image ........................................... 30

Closing ZEN 2.1 ........................................................................................................................ 31

Downstream Image Processing and Analysis .......................................................................... 32

Open .................................................................................................................................... 32

Display Adjustments ............................................................................................................ 32

Add Scale Bar or Arrows ...................................................................................................... 34

Distance Measurements ..................................................................................................... 35

Cropping Region of Interest (ROI) ....................................................................................... 36

Export .................................................................................................................................. 37

Other Guidelines ......................................................................................................................... 40

Further Protocols, Tutorials and Guidelines ............................................................................... 43

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Brief Description

Axio Zoom.V16 is a stereo microscope that successfully combines a large field of view,

zoom, and working distance. Axio Zoom.V16 combines a 1x zoom with a numerical

aperture of NA 0.25. It achieves a considerable numerical aperture for a medium zoom

range: superior fluorescence brightness in large object fields.

Axio Zoom.V16 is the fluorescence zoom microscope fully automated for large field

visualization having a motorized zoom from 0.7x to 11.2x, motorized Z focus and easy-

to-use system control panel.

Reference

Zeiss Axio Zoom.V16

Location

Room 5.25 (Microscopy Cluster), 5º floor, Instituto de Tecnologia Química e Biológica

António Xavier (ITQB NOVA)

Suggestion for “Materials and Methods”

Images were acquired on a Zeiss Axio Zoom.V16 stereo microscope equipped with a

Zeiss Axiocam 503 mono CCD camera and controlled with the Zeiss Zen 2.1 (blue edition)

software, using the 1x 0.25 NA objective, the fluorescence filter sets GFP + mRFP and

the Bright Field optics.

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Suggestion for “Acknowledgements”

This work was partially supported by PPBI - Portuguese Platform of BioImaging (PPBI-

POCI-01-0145-FEDER-022122) co-funded by national funds from OE - "Orçamento de

Estado" and by european funds from FEDER - "Fundo Europeu de Desenvolvimento

Regional”.

People and Contacts

Adriano O. Henriques, Microbial Development Group (aoh@itqb.unl.pt)

Mónica Serrano, Microbial Development Group (serrano@itqb.unl.pt)

Carolina Cassona, Microbial Development Group (ccassona@itqb.unl.pt)

Fernando Cruz, Microbial Development Group (fernandocruz@itqb.unl.pt)

Phone +351 214 469 524

Extension 1524

Optics

Transmitted Light:

• Bright Field

• Dark Field

• Oblique Light

Incident Light:

• Fluorescence

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Features and Specifications

• Incident Light Source: HXP 200 C illuminator (w/ integrated electronic shutter)

• Illumination: HXP 200 (200 W mercury short-arc reflector lamp)

• Fluar illuminator Z mot: Motorized reflector turret for up to four Z reflector

modules (filter sets)

• Motorized Stand 300: Focus motor 3 w/ central profile column placed on a

Transillumination base 300 (Transmitted Light Source)

• System Control Panel: SYCOP 3 (joystick, scroll wheels, buttons, and a

touchscreen into a handy, mobile control unit)

• Electronic Module: EMS 3 (for connecting SYCOP3)

• Objective nosepiece Z

• Eyepieces: PL 10x/23 Br. foc.

Objective

• Magnification: 1x

• Zeiss System: PlanNeoFluar Z

• Class: Achromatic objective

• Numerical Aperture (A): 0.25

• Free Working Distance (mm): 56

• Pixel Size for 0.7x (µm): 6.4857

• Pixel Size for 11.2x (µm): 0.4054

Notice that the zoom microscope is equipped with two eyepieces PL 10x/23 Br. foc.

Furthermore, a camera adaptor 60N-C1 1x is also installed. In general, the Axio

Zoom.V16 has a motorized zoom from 0.7x to 11.2x

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Filter-Sets

Filter Cube Excitation Filter Dichromatic Mirror Emission Filter Emission Colour

GFP1 BP 470/40 495 BP 525/50 Green

YFP1 BP 500/25 515 BP 535/30 Green/Yellow

mRFP1 BP 572/25 590 BP 629/62 Red

1High efficiency (HE) filter set: These filters are distinguished by particularly steep cut-off edges and extremely high transmittances

BP stands for bandpass filter

Additional Equipment

Camera

• Brand: Zeiss®

• Type: CCD Cameras

• Model: Axiocam 503 mono (D)

• Adaptor: 60N-C1 1x (magnification)

• Features: Sensor CCD; 2.8 megapixels (1936 x 1460 active pixels); 4.54 µm pixel

size; 38 Frame Rate @ 1936 x 1460 (frames per sec)

Computer

• Type: Desktop

• Brand: Asus®

• Model: i9S

• Features: Intel I5 4460 up to 3.2 GHz (quad-core); 8GB RAM; 1TB HDD

• OS: Windows 7 Professional

• Available Software: Zeiss Zen 2.1 (blue edition)

Monitor

• Brand: Samsung

• Model: S24C450B

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System Overview

WARNINGS and Advice

• Before you get started for the first time on this microscope consult the

responsible people for proper training

• ONLY turn ON the lamp if it has been off for more than one hour

• Do not turn OFF the lamp if someone else will be using the microscope in the

next few hours. In order to avoid unnecessary working hours or usage constraints,

please inform the next user about the microscope occupancy

• You must turn ON THE LAMP FIRST and then the remaining devices

• THE LAMP must be the LAST TO TURN OFF

• Never LOOK DIRECTLY into the beam paths as they are capable of permanently

damaging the human eye

• Make sure you have everything set (as for instance, samples, plates, etc) before

starting the observations

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• Automatic movements can be interrupted by pressing the STOP button. Prevent

the objective from colliding with the specimen or transillumination base. The end

positions for the motorized range of movement should be set when appropriate

• When the motorized focusing drive is lowered, there is the risk of pinching

fingers in the working area. Do not reach into the working area or beneath the motorized

focusing drive during lowering

• The diaphragm slide should initially be at the rear stop in order to achieve a large

homogeneous luminous field. Pull it slowly to the front while observing the microscopy

image to achieve the desired effect

• The transmitted light can be completely covered with the diaphragm slide (front

stop) to achieve a dark background, e.g. for fluorescence illumination

• Slight careful deflection of the joystick results in fine focusing, while full

deflection produces faster focusing movements as a course screw would produce

• If you personalized some ZEN 2 pre-setting, reset the software for the default

definitions. Do not save any alteration to prior experiment sets

• Make sure you have the correct tube opening regarding the intended light path

(eyepieces or camera)

• Save all data into your own storage device or cloud as we routinely clean the

computer’s data

• Do not forget to register your utilization in the logbook

• A free software license is available for ZEN lite at ZEISS website. Install and use

such version in your own personal computer for downstream image analysis (see

Downstream Image Processing and Analysis)

• Contact the responsible people in case of any doubt

Potential Techniques

• Large-Field Observations

• Large-Field in vivo Timelapses

• Biofilm Observation

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Set Up

Sample Preparation

Colony and Biofilm Observation

This stereoscope allows visualization of colonies and biofilms previously

grown/developed onto plates supplemented with any media. Simply place the plate in

the centre of the transillumination base. Do not remove the cover.

Initial Set Up

1. Before you start, ensure the lamp has been off for more than one hour

2. Switch on the devices in the following order:

2.1. Incident Light Source HXP 200 C (Lamp)

2.2. Transmitted Light (turn the rotary knob clockwise)

2.3. Electronic Module EMS 3 (turn the device on in the back)

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2.4. Computer

3. All motorized components will run through an initializing phase, including the

SYCOP 3 (System Control Panel)

4. Meanwhile, in the computer’s desktop directory, run ZEN (blue edition)

and select ZEN Pro

5. Place the specimen in the centre of the round insert plate

6. Ensure that the aperture diaphragm is completely open. To do this, turn the

rotary knob up (on the right-hand side of the microscope)

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7. Adjust the tube towards camera image acquisition by pulling out the control bar.

Alternatively, if you only intend to use the eyepieces for visualization, please consult

other guidelines (See Operating Manually)

8. Although you can operate the microscope in manual mode, without using ZEN

2.1 (See Operating Manually), we strongly advise you to use the available software for

observation and image acquisition (Go to Single Image Acquisition or Visualization)

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Final Set Up

1. Before you switch off the devices confirm the next user and if so, ask whether he

or she will be using the microscope in the next hour

2. Remove the specimen from the transillumination base

3. If there is an interval of more than one hour to the next user, switch off the

devices. Press the Shut Down button on the Home page of SYCOP 3 touchscreen

4. When the safety query is answered by pressing the YES button, the

system will be shut down, while the remaining devices will be in the standby mode

5. Then, switch off the devices in the following order:

5.1. Electronic Module EMS 3 (turn the device off in the back)

5.2. Transmitted Light

5.3. Incident Light Source HXP 200 C (lamp)

6. Save all data into your own personal storage device or cloud and switch off the

computer (see FIRST Closing ZEN 2.1)

7. Sign your registration in the logbook

8. Take everything you brought and keep the room and desk clean for the next user

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ZEN 2.1 (blue edition) Guidelines

The ZEN 2.1 (blue edition) can be equipped with several modules, applications and

licenses. This guideline is strictly specific for the ZEN 2.1 (blue edition) version available

on the microscope’s computer. In other words, only for ZEN 2 pro on the computer.

The ZEN 2.1 user interface is divided into three main areas. Via the tabs in the Left

Tool Area (4) you can access all the main tools for microscope control (Locate tab),

acquisition (Acquisition tab), image processing (Processing tab) and image analysis

(Analysis tab). The Centre Screen Area (5) is used to display your images, while the Right

Tool Area (6) provides you with an overview of all open documents and is used for

advanced file management.

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Single Image Acquisition or Visualization

1. Go to the Locate tab

2. In the Favorites section, select the contrast method or filter set you would like

to acquire or visualize

The FL off mode activates the transmitted light and closes the shutter of incident

light (switching off fluorescence optics). To fully activate the transmitted light, you

should also open the diaphragm slide until the rear stop (in the Transillumination base

300), so that a large homogeneous luminous field can be obtained.

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You can now pull the diaphragm slide slowly to the front for adjusting the light

intensity. Notice that the bright field contrast method is regularly set as default (See

Other Guidelines -> Contrast Methods and Filter Sets).

Finally, once you finished the visualization in the FL off mode (transmitted light

illumination), do not forget to close the transmitted light by moving the diaphragm slide

until the front stop.

3. Click in the Live button. Once the Live mode is activated the camera live image

will be displayed in the Centre Screen Area

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4. Click in the blue header of the Camera tool. Then, in the Settings section, select

the adequate exposure time or Auto Exposure option.

Although, it is possible to increase or decrease the Exposure Time during visualization

as function of the fluorophores’ signal. To get back to the default settings, click in the

Default button.

5. Start focusing your sample using the SYCOP 3. You should move the specimen

carefully to find interesting and prominent detail.

With the minimum magnification, use the joystick and side wheels to swipe the

motorized focusing down or up. Repeat the process for consecutive magnifications

Joystick Robust Forward Tilt Coarse Focusing Moving upwards

Joystick Robust Backward Tilt Coarse Focusing Moving downwards

Joystick Soft Forward Tilt Fine Focusing Moving slightly upwards

Joystick Soft Backward Tilt Fine Focusing Moving slightly downwards

Wheels Swipe Forward Fine Focusing Moving extremely slightly upwards

Wheels Swipe Backward Fine Focusing Moving extremely slightly downwards

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To increase or decrease the objective magnification in the SYCOP 3, go to the

Microscope tab and select the magnification in the Function sub-tab

1 – Joystick

2 – Side Wheels

Hint: When focusing on the Bright Field contrast method try to adjust slightly the

diaphragm slide in order to obtain greater detail and contrast

Hint: If you are getting problems focusing the sample, use the Min/Max

button in the Histogram (Centre Screen Area -> Display -> Histogram). This will adjust

the gray levels which may be responsible for darker, dim or overexposed images (See

Downstream Image Processing and Analysis -> Display Adjustments)

6. After focusing the sample, click in the Snap button to acquire the first

image

2 2

1

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Multidimensional Image Acquisition (Multi-Channel Images)

1. Go to the Acquisition tab

You can create a new blank experiment. Alternatively, you can use one of the pre-

set experiments. Try to follow the protocol until the end and then look at the pre-set

experiments that may be useful for your experiment. In this way, we can keep the

workspace organized and without redundancy.

You can use and alter the available pre-set experiments as long as those alterations

are not saved at the end! We advise this option for short-term experiments

2. To create a new, "empty" experiment, click in the Options button of the

Experiment Manager section. The Options dropdown list will open.

3. Click on the New entry.

4. Enter a name for the experiment, e.g. "experiment_3_channel"

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5. To create the experiment, click in the Save button

You have created a new blank experiment. For any change in the experiment, an

asterisk (*) will appear after the file name. This means that the experiment was modified

and not saved. Save your experiments from time to time to ensure that your settings are

not lost.

6. Start focusing the sample (See above ZEN 2.1 (blue edition) Guidelines -> Single

Image Acquisition or Visualization)

7. Now in the Multidimensional Acquisition group, click in the blue header of the

Experiment Designer tool

8. Make sure to box the Enable Multi Block Execution option

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9. In the Acquisition Block section, click in the Add New button.

a) Add as many blocks as contrast methods/filter sets you would like to

acquire. For instance, to acquire images of Bright Field, GFP, mRFP and YFP, four blocks

must be added. This approach will generate one image for each contrast method and

filter set.

Later, you will be able to combine two images at a time into one using ZEN 2.1

(See ZEN 2.1 (blue edition) Guidelines -> Channel Combine or Image Overlay)

b) Alternatively, you can add only one block. This approach will generate a

multichannel image of all selected filter sets. Combine only filter sets into a single block

though, as it is required one block for each single contrast method.

In the end, you can extract individual fluorescence images of a multichannel

image (See ZEN 2.1 (blue edition) Guidelines -> Extracting Single Fluorescence Images of

a Multichannel Image)

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c) If you have selected multiple contrast methods that require distinct

illumination methods (e.g. Bright Field and Fluorescence), you must use the Wait block.

To set a Wait block, just click in the Wait button.

As it is strictly required to operate the diaphragm slide between transitions of

transmitted and incident light (e.g. from bright field to fluorescence GFP), the Wait block

will make a pause during the experiment.

At the pause, you should set the diaphragm slide in agreement with the next

contrast method.

To switch the order among blocks, just click in a block and move it into the

desired position of the timeline of events.

10. Box the 1 separate image document / acquisition block option to create an

image for each block of the timeline of events

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11. Select the first block in the timeline of events

12. Now in the Acquisition Parameter group, click in the blue header of the Channels

tool

13. Click in the + button to open the Add Dye or Contrast Method dialog

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14. Select the desired filter set (from the Dye Database) or contrast method (from

the Contrasting Methods). You can search for a dye by entering its name (or starting

letter) in the Search input field (See above the available Filter-Sets)

15. Click in the Add button at the bottom of the dialog or simply double-

click an entry

IF you have previously selected one single channel image per block, click in the Close

button of the Add Dye or Contrast Method dialog and move to the next step.

IF you have previously selected a multichannel image by setting only one block for

acquiring multiple filter sets (multichannel image), repeat this step to add the remain

channels. Close the dialog at the end.

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16. Once you have selected a dye(s) or contrast method, one will be displayed in the

Channels tool

17. Set the Exposure Time for each channel, just right beneath the channels list.

There will be more than one channel in a multichannel block. Thus, the Exposure Time

should be set one channel at a time

18. Repeat the steps 12-17 for the remaining blocks. Once you have finished

assigning all channels and contrast methods, move to the next step

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19. To save the experiment together with all the settings, click in the Options

button of the Experiment Manager section and select the Save entry

!!! Save ONLY self-made-experiments!!! Do not save previously pre-set experiments,

unless they are yours

20. Finally, select the first block of the timeline of events (in the Experiment Designer

tool) and click in the Start Experiment button

21. The last image to be acquired will be displayed in the Centre Screen Area. The

remain images will appear in the right-side Right Tool Area

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Channel Combine or Image Overlay

1. Go to the Processing tab

2. Click in the Single button

3. Click in the blue header of the Method tool

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4. Click in the Utilities group to display the dropdown list of tools included in this

category

5. Search for the Add Channel tool and click in it

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6. Click in the blue header of the Input tool

7. Select images of channels you would like to combine. To do so, click in the small

preview image within the Input tool. You will see a preview of all open images. To select

an image, just click in it

8. Yet in the Input tool, select the Switch to Output option only

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9. Click in the blue header of the Output tool and select the Create New Output

option only. Additionally, you can name the new image as you like by clicking in the

Naming button

10. Finally, click in the Apply button. The image combined will be

displayed in the Centre Screen Area.

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Extracting Single Fluorescence Images of a Multichannel Image

1. Select the Processing tab. Open the Method tool and under Utilities select the

entry Create Image Subset

2. In the Image Parameters group, open the Input tool and select the multichannel

image as input

3. In the Method Parameters group, open the Parameters tool and click in the

Channels entry. For instance, deactivate the red and green/yellow fluorescence

channels by clicking in the corresponding channels to extract only a single green

fluorescence image

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4. Finally, click in the Apply button. The single channel image will be

displayed in the Centre Screen Area

Closing ZEN 2.1

1. To end ZEN 2.1 software, go to File -> Exit. Alternatively, you can press ALT

+ F4 on your keyboard or click in the Close icon of the program bar.

!!!Note: If you haven’t saved your files, the Save/Keep Documents dialog

will open before the program closes. Select files you want to save or unselect

files you don’t want to save.

Save only documents that are actually yours, and not pre-set experiments

that belong to others or light path settings of the favorites filter sets.

Images only created for

testing purposes which

can be discarded

Experiment of another

user which should not be

saved!

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Downstream Image Processing and Analysis

A free software license is available for ZEN lite at ZEISS website

(https://www.zeiss.com/microscopy/int/products/microscope-software/zen-lite.html). Install

and use such version in your own personal computer for downstream image analysis

Open

1. Go to File -> Open

2. Search for the .czi files previously saved in the directory dialog

3. The selected files will be displayed in the Centre Screen Area and Right Tool Area

Display Adjustments

1. In the Dimensions tab of the Centre Screen Area, activate the Range Indicator

checkbox. This will mark in red or blue overexposed (too bright) or underexposed (too

dark) areas, respectively

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2. On the Display tab click in the 0.45 button. The display curve will be

adapted to a gamma value of 0.45. This will set the optimum colour presentation. If you

do not see this button activate the Show all mode

3. Move the controls left and right under the multiple curves in order to directly

adjust the values for Brightness (White), Gamma, and Contrast (Black) in the live image

1 - Contrast (black point) control

2 - Gamma control

3 - Brightness (white point) control

1 2 3

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Add Scale Bar or Arrows

1. In the Center Screen Area, select the Graphics tab

2. Click in the Scale Bar or Draw Arrow buttons. The scale bar will

appear directly in the image and in the Annotations/Measurements as an entry. To set

an arrow, the button will turn blue and then you can draw an arrow over the image

You can access to numerous formatting possibilities by right-clicking in the

annotation (scale bar, arrow, etc). This will open the context menu. Select Format

Graphical Elements… in this dialog

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Distance Measurements

1. Go to Graphics -> Line

2. Draw the line over the object you would like to measure

3. In the Center Screen Area, select the Graphics tab. An entry of the Line type will

appear together with the positions and sizes (these measurements are not the distance

length between the two-point edges of the line)

4. To display the distance length, box the M column and the measurement will be

displayed in the image under the line

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Cropping Region of Interest (ROI)

1. Go to Graphics -> Draw Region of Interest

2. Draw a square or rectangular region over the region of interest

3. Right-click in the drawn region. This will open the context menu. Select Create

Subset Images from ROI in this dialog

4. The selected region will be displayed as a new image in the Centre Screen Area.

Besides, the image document will appear in the right-side Right Tool Area

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Export

1. Go to the Processing tab

2. Click in the Single button

3. Click in the blue header of the Method tool

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4. In the Method tool, open the Export/Import group and select the Image Export

method

5. Under Method Parameters group, select the Parameters tool, and set the

desired export settings, such as file type, quality, export folder, …

6. Click in the blue header of the Input tool

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7. Select the images you would like to export. To do so, click in the small preview

image within the Input tool. You will see a preview of all open images. To select an

image, just click in it

8. Yet in the Input tool, select only the Switch to Output option

9. Finally, click in the Apply button.

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Other Guidelines

Using the STOP button

The STOP button is for instantaneous deactivation of a moving motorized focusing

drive to prevent collision with a specimen on the latter.

1. Press the STOP button to switch off the focusing drive. The STOP button is on the

left-hand side of the drive

2. The STOP button engages and the focusing movement is immediately

interrupted

3. To unlock the STOP button, pull it out again. The STOP button must be unlocked

to restart the focusing drive

Operating Manually

1. Place a specimen in the centre of the round insert plate

2. Pay attention to the following operation modes:

a. To use only the incident light and respective filter sets, operate solely with

the Fluar illuminator Z mot and make sure the diaphragm slide is closed

b. Alternatively, to use only the transmitted light, press the number 4 in the

Fluar illuminator Z mot and operate with the diaphragm slide and setting wheel

c. Finally, to switch between lights, operate with both Fluar illuminator Z

mot, setting wheel (See Other Guidelines -> Contrast Methods and Filter Sets) and

diaphragm slide

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3. Ensure that the aperture diaphragm is completely open. To do so, turn the rotary

knob up (on the right-hand side of the microscope)

4. Adjust the dioptre-setting ring in the focusing eyepieces if known, otherwise set

it to "0" and do not remove your glasses or contact lenses. The white and red dot stand

for "0" (without eyepiece reticule)

5. Check that the eyepieces are completely housed in the tube

6. Adjust the tube towards eyepiece visualization by pushing in the control bar

7. Adjust the interpupillary distance by turning the eyepiece sockets. When looking

into the eyepieces with both eyes only one unclipped circle of light (object field) should

be visible

8. Use the SYCOP 3 to focus and zoom the specimen (See ZEN 2.1 (blue edition)

Guidelines -> Single Image Acquisition or Visualization)

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Contrast Methods and Filter Sets

Whether selecting a contrast method or a filter set the following table presents only

manual operations that should be avoided with ZEN 2.1 software in most of the cases.

Contrast Method/

Filter Set

Symbol/

Number Control Device Instruction

Bright Field BF/4

Setting Wheel/ Fluar

Illuminator Z mot./

Diaphragm Slide

In the Fluar Illuminator Z mot., press the number 4.

Next, turn the setting wheel towards the position

marked with BF. Open the diaphragm slide

Higher Contrast

Bright Field BF+/4

Setting Wheel/ Fluar

Illuminator Z mot./

Diaphragm Slide

In the Fluar Illuminator Z mot., press the number 4.

Next, turn the setting wheel towards the position

marked with BF+. Open the diaphragm slide

Dark Field DF/4

Setting Wheel/ Fluar

Illuminator Z mot./

Diaphragm Slide

In the Fluar Illuminator Z mot., press the number 4.

Next, turn the setting wheel towards the position

marked with DF. Open the diaphragm slide

Fluorescence: GFP 1 Fluar Illuminator Z

mot.

Cover the transmitted light illumination by moving the

diaphragm slide towards the front stop position. In

the Fluar Illuminator Z mot., press the number 1.

Fluorescence: YFP 2 Fluar Illuminator Z

mot.

Cover the transmitted light illumination by moving the

diaphragm slide towards the front stop position. In

the Fluar Illuminator Z mot., press the number 1.

Fluorescence:

mRFP 3

Fluar Illuminator Z

mot.

Cover the transmitted light illumination by moving the

diaphragm slide towards the front stop position. In

the Fluar Illuminator Z mot., press the number 1.

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1 – Setting Wheel

2 – Diaphragm Slide

3 – Fluar Illuminator Z mot.

Further Protocols, Tutorials and Guidelines

Contact the responsible people to acquire the following tutorials and instruction

manuals:

• Zeiss Zen 2 lite

• SYCOP 3 System Control Panel Operating Manual

• ZEN 2.1 (blue edition) User Guide

• Axio Zoom.V16 Operating Manual

• ZEN 2012 (blue edition) Quick Guide: Multi Dimensional Imaging

• ZEN 2012 (blue edition) Quick Guide: Import/Export

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