stream sampling assessing water quality through the collecting of macro-invertebrates found on woody...
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Stream Sampling
Assessing water quality through the collecting of macro-invertebrates found
on woody debris
Sample Sites
• This method will work in any stream shallow enough to wade in safely
• Areas without trees near the stream may lack sticks.
• It may be possible to create “artificial” samples by placing suitable (see next slide) sticks in the stream a few weeks in advance of sampling
Procedure:In the field
• From each site used, two samples of woody debris are collected, each consisting of two to three pieces
• Each piece should be about 12-14 inches long and 0.25 to 1.5 inches (5-35 mm) diameter
• Sticks selected should be in the current, neither on the bottom nor above the surface
Sampling 2.
• Cut the samples under the water if possible, using pruning shears (or a pruning saw for very thick pieces)
• Place the sticks in a two-gallon zipper seal plastic bag, one bag for each sample of 2-3 sticks
• Place a label in the bag
Label for sample in the field
GREAT EGG HARBOR RIVER
SITE # __________________
DATE: ___________
SAMPLE # __________
SUBSTRATE:___________
These can be made in advance, printed on a laser printer. Otherwise, use #2 pencil
Size: 1.25 x 2.25 inches
Procedure:In the field
• Specific conductance (S) and pH are measured in the field
• The most accurate readings are made using electronic meters
Procedure: In the Lab
• Take stick samples back to the lab and wash with a soft scrub, nylon bristle brush into a bucket
• Keep samples separate throughout the preparation and analysis
Lab sample preparation 2
• Pour washed off material through an 0.5 mm sieve
• Transfer contents to 80% ethanol and place a label in the container
Label for washed sample
NJ: ATL. CO. NACOTE CREEK
Morses Mill below Lake Fred
25 OCTOBER 2004
SAMPLE # __________
SUBSTRATE:___________
These can be made in advance, printed on a laser printer. Otherwise, use #2 pencil
Size: 1 x 1.75 inches
Procedure:In the lab
• After scrubbing, examine the cleaned sticks under a stereo microscope for any remaining invertebrates
• Place these in a separate, labeled container in 70% ethanol with 5% glycerin.
Sample sorting: In the lab
• Place small amounts of debris from sample in 0.75 x 0.75 x 18 inch tray in ethanol
• Examine under stereo microscope, removing all invertebrates
• Preserve invertebrates in a separate, labeled container in 70% ethanol with 5% glycerin.
• Discard plant debris, sediment, etc.
Sorting and labeling taxa
• After macro-invertebrates have been sorted from debris, sort each sample into taxa as indicated on the data sheet
• Make label for each taxon from each sample
• Place each taxon and label in a separate vial of 70% ethanol with 5% glycerin
TRICHOPTERADATE: Aug 2004GREAT EGG HARBOR RIVERSITE__________SAMPLE #___SUBSTRATE: wood
These can be made in advance, printed on a laser printer. Otherwise, use #2 pencil
Size: 1.25 x 2.25 inches
Sample # Date Site
Taxon 8 char name Desc. # adult# larvae &
pupae Total Notes
Simulidae Simulida black flies
Chironomidae Chironom midges
Coleoptera Coleopte beetles
Trichoptera Trichopt caddisflies
Plecoptera Plecopte stoneflies
Ephemeroptera Ephemero mayflies
Megaloptera Megalopt fishflies
Odonata Odonata dragon & damselflies
Crustacea Crustace scuds, pillbugs
Gastropoda Gastropo snails
Pelecypoda Pelecypo clams
Worms Worms
Leech Leech
Mite not analyzed
Eggs not analyzed
Cases not analyzed empty cases
Limpets not analyzed tiny ??
Other
Other
Other