superficial mycoses
TRANSCRIPT
SUPERFICIAL MYCOSES
KIMAIGA H.O
MBChB (University of Nairobi)
Superficial Mycosis
• Dermatotophytoses
• Superficial candidiasis
• Pityriasis versiclour(tinea versicolor)
• White piedra
• Black piedra
• Otomycosis
• Mycotic keratitis
Dermatophytoses
• Diseases of superficial keratinized structures- nail, hair, skin
• Filamentous/ Monomorphic fungi- Always in mould form
• Pathogenic genera: • Trichophyton- Infect skin, hair and nails (Mneumonic Tri)• Microsporum - Infect hair and skin• Epidermophyton - Infect nails and skin
• Classified as;• Anthrophillic - restricted to man• Zoophilic- in animals, inflammatory reaction in humans with
contact with animals• Geophillic- from the soil, occasionally infects man and
animals
Pathogenesis• Infection can be by direct or indirect transfer (fragment of
keratin containing the infective particle)• No evidence of natural immunity• Factors that encourage invasion- increased environmental
humidity and CO2 content• Resistance to infection is determined by
• Production of inhibitory FAs (fatty acids)• Rate of epithelial cell turnover• T-cell mediated immunity• Unsaturated transferrin
• Keratin affected by enzymatic digestion and mechanical pressure
• Hyphae grow into newly differentiated keratin as it formed• Branching hyphae & may break up to arthroconnidia
particularly in infected hair
• Species of fungi determine type of lesions:
• Skin- dry scaling/ hyperkeratosis irritation, erythema, vesicles, pustules, ulceration
• Nail- discolouration, thickened, raised
• Scalp infection- scaling, hair loss, lesions (kerions)
“Id” reaction• An immunological allergic reaction to
circulating fungal antigens• Patients with inflammatory infection develop a
secondary rash known as dermatophytid (“id”)• Those with foot ringworm will have vesicular
eczema on the hands• Those with scalp ringworm develop follicular rash
on the trunk
Epidemiology/ Diseases
• Common in tropics• Types:
• Tinea corporis (smooth skin)• Tinea cruris (groin/ jock itch)• Tinea capitis (scalp)
• Favus is more serious, often from animals and causes permanent hair loss
• Tinea pedis (feet/athlete’s foot) - also candida species gram negative bacteria
• Tinea unguium(nail plate)• Tinea barbae (beard)• Tinea manuum(palms)• Tinea faciale
• NB: these are conditions not genera and species
Fungal
Fungal
Clinical Manifestations
T. Corporis manifestations• Trichophyton species, microsporum species
and epidermophyton floccosum• Frequently from a pet, infection from another
body site, person to person• Scaly, itchy rash on trunk legs arm• Itching, dry, circular, scaling lesions• May be very large, affecting the back and chest• In AIDs patients- extensive or follicular forms• Usually zoophilic, occasionally geophillic
T. Capitis manifestations• Several tricophyton species, and microsporum
species• Anthrophillic – easily transmissitted from child
to child• Endemic in most African communities• Generally confined to prepubertal children• Diffuse or circumscribed areas of hair loss• Minimal scaling, hair broken at scalp level, leaving
black swollen dot at hair follicle
• Zoophilic • More inflammation, scaling and hair loss• Itchy inflammatory crusts cover lesion• In AIDs adult T. capitis may be seen
T. Pedis manifestations
• Anthrophillic- T. rubrum, E. floccosum
• Itchy, +/- pain, +/- erosions of web spaces
• Severe erosion with greenish discoloration suggests pseudomonas
Onchomycosis
• Toe nails usually
• Usually together with sole or web space infection
• Anthrophillic
• Nail thickened & opaque, distal erosion of nail plate in chronic cases
• T rubrum, T interdigtale, Fusorium spp., Acremonium spp.
T. Cruris
• Skin of groin and pubic area
• Anthrophillic- T rubrum, E floccosum
• Usually from another body part
• Highly contagious sharing towels e.t.c
• One or more rapidly spreading erythematous lesions with central clearing on the inside of thighs, intense pruritus
• Lesions with raised erythematous border and brown scaling
• Infection may extend locally and spread to other body sites
Lab Diagnosis
• Sample
• Nail clippings
• Skin scrapings (clean skin- 70% alcohol - ↓ bacterial flora)
• Plucked hair collected in folded squares of black paper or card fastened with clip
• Macroscopic exam (Wood’s lamp)
• Detect fluorescence in infected hair, scalp lesions
• Direct microscopy• Microscopy of wet mounts of KOH preparations
(10-30% KOH digestion 15 mins)
• Look for hyphae or arthrospores
• Calcofluor white- optical brightener under fluorescence microscope - enhances fungal elements
• Microsporum fluoresces a bright yellow green (Wood’s lamp)
• Direct microscopic exam of hair roots and skin softened with KOH shows hyphae, arthrospores and distinctive patterns of hair invasion
• Arthroconidia retained in hair shaft - endothrix invasion-M. nanum, T. tonsurans
• Arthroconidia on a sheath surrounding hair shaft -ectothrix invasion-M.canis, gypseum. T. verrucosum
Shaft of hair with KOH Skin scrapping with KOH
KOH preparation of hair KOH preparation of skin
Hyphae in skin:
• Culture
• For isolation and ID of genus and species
• On SDA for 10-14 days
• Check for colonial morphology
• Lactophenol blue staining on a portion of the colony
• Look for micronidia and macronidia
• Dermatophyte species produce 2 types of asexual spores: macroconidia & microconidia
Treatment• Topical therapy
• Nonspecific – Whitfield’s ointment
• Specific – Creams, lotions, shampoos
•Azole derivatives- Clotrimazole, ketoconazole etc.
• Oral antifungals if skin contact hurts– required for nail scalp and severe skin infection includes Griseofulvin, Terbinafine, Itraconazole, Fluconazole
• Onchomycosis• Griseofulvin for 12-18 months-high relapse rates
• Oral terbinafine or itraconazole
Prevention
• Involves minimizing transmission from infected individuals especially through indirect spread from contaminated items
Pityriasis/ tinea versicolor
• Caused by Malassezia furfur, normal skin flora
• Usually in adults and less common in children
• Common in roofing or construction workers in warm climate , exposed to sun, who are constantly wet and sweat hence infection can reoccur even after treatment.
• Associated with immonusuppression due to organ transplantation. Not known association with AIDS
Presentation
• Superficial infection of keratinized cells
• Hypopigmented (in dark skin) or hyperpigmented (in light skin)spots on the chest and back
• Coalesce with time to form confluent patches
• Fungemia in premature infants on IV lipid supplements
Sebborhoeic dermatitis
• Yeast found in large quanties in scales of dermatitis and in dandruff
• AIDS patients –Early sign of CD suppresionbut also commonly in health persons
• Erythema and greasy scale- Eyebrows and eyelashes, scalp, nasolabial folds, behind ears, over sternum.
Lab diagnosis
• Microscopy• Oval, budding yeast and short filaments
• Culture• A lipid supplement is required in medium
• Incubated at 32-340C
• Small yello-creamed colonies develop within 1 week.
Prevention
• General cleanliness
• Avoid sharing personal items- combs, towels, clothes
White piedra
• Softwhite grayish nodules on hair shafts mainly axillae
• Caused by a yeast – Trichosporum beigelii
• Shaving off infected hairs is satisfactory treatment
Otomycosis
• Chronic otitis externa
• Patient has a lot of discomfort, itch on affected site
• Aspergillus nigger is the most common species involved
• Species isolated on SDA, sporulation after 2 days
• Cleaning and topical therapy with azole derivatives
Mycotic keratitis
• Fungal infections of the cornea
• Can be secondary to injury or bacterial infections, use of steroids
• Mainly caused by saprophytic moulds e.g. Aspergillus and Fusarium species