supernova for cali technique
TRANSCRIPT
SUPERNOVA A MONOMERIC PHOTOSENSITIZING FLUORESCENT PROTEIN FOR
CHROMOPHORE-ASSISTED LIGHT INACTIVATION
KIWAMU TAKEMOTO ET AL. 2013SCIENTIFIC REPORTS
PHOTOSENSITIZERS
Bulina et al., 2006
CALI CHROMOPHORE-ASSISTED LIGHT INACTIVATION
Photosensitizer ProteinTarget Protein
intense light irradiation Target inactivation
Cellular Death
PHOTOSENSITIZERS
Li et al., 2012
KILLER RED VS SUPER NOVA
Takemoto et al., 2013, Bulina et al., 2006
579 nm610 nm
SuperNova KillerRed EGFP
Cx43
Keratin
Fibrillarin
KILLER RED VS SUPER NOVA
Scale bar 10µmTakemoto et al., 2013,
EXPERIMENTAL RESULTS
0 15 30
45 60 75 90
Takemoto et al., 2013, Bulina et al., 2006Magnification 100x
EXPERIMENTAL RESULTSActin-GFP Cofilin-SuperNova
BeforeCALI
AfterCALI
Takemoto et al., 2013Scale bar 10µm
CALI APPLICATIONS
Metaphase Early Cytokinesis Late Cytokinesis Death Survival
Ou et al., 2012
CALI IN C.ELEGANS
Scale bar 5µm Scale bar 2,5µm
WT CALI QR.a QR.a (pig-1) QR.p
REFERENCES
Wei Li, Nico Stuurman, Guangshuo Ou. (2012). Chromophore-assisted laser inactivation in neural development. Neuroscience Bulletin. 28 (4), 333–341.
Maria E Bulina, Konstantin A Lukyanov, Olga V Britanova, Daria Onichtchouk, Sergey Lukyanov & Dmitriy M Chudakov. (2006). Chromophore-assisted light inactivation (CALI) using the phototoxic fluorescent protein KillerRed. Nature Protocols. 1 (2), 947-953.
Maria E Bulina, Dmitriy M Chudakov, Olga V Britanova, Yurii G Yanushevich, Dmitry B Staroverov, Tatyana V Chepurnykh, Ekaterina M Merzlyak, Maria A Shkrob Sergey Lukyanov & Konstantin. (2006). A genetically encoded photosensitizer. Nature Biotechnology. 24 (1), 95-99.
Guangshuo Ou, Nico Stuurman, Michael D’Ambrosio, Ronald D. Vale. (2010). Polarized Myosin Produces Unequal-Size Daughters During Asymmetric Cell Division. Science. 330, 677-679.
Takemoto, K., Matsuda, T., Sakai, N., Fu, D., Noda, M., Uchiyama, S., ... & Nagai, T. (2013). SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation. Scientific reports, 3.
Look for diagnosis.com (2014). Chromophore-Assisted Light Inactivation (CALI) using the phototoxic fluorescent protein KillerRed. Available: http://jcs.biologists.org/content/127/8/1621/F1.large.jpg.