suppl. fig. 1a. assessment of macrm commutability by twin plot analysis (glucose) : patient sera...
TRANSCRIPT
Suppl. fig. 1a. Assessment of MacRM commutability by twin plot analysis (Glucose)
: Patient sera (n=30) : NIST SRM917c : MacRM-001
50
100
150
200
250
50 100 150 200 250
(mg/dL)
A&
T ,
Glu
cose
oxi
dase
ele
ctro
de (
A)
y =R =
1.033x - 1.60.9999
Hitachi , Hexokinase G6PDH (A)
50
100
150
200
250
50 100 150 200 250
(mg/dL)
Sie
men
s ,
Hex
okin
ase
G6P
DH
(F
)
Hitachi , Hexokinase G6PDH (A)
y =R =
1.012x + 3.80.9997
50
100
150
200
250
50 100 150 200 250
(mg/dL)
Hita
chi ,
Glu
cose
deh
ydro
gena
se
Hitachi , Hexokinase G6PDH (A)
y =R =
1.015x - 0.20.9998
50
100
150
200
250
50 100 150 200 250
(mg/dL)
Hita
chi ,
Hex
okin
ase
G6P
DH
(C
)
Hitachi , Hexokinase G6PDH (A)
y =R =
1.032x + 0.80.9999
50
100
150
200
250
50 100 150 200 250
(mg/dL)
Ark
ray
,G
luco
se o
xida
se e
lect
rode
(B
)
Hitachi , Hexokinase G6PDH (A)
y =R =
1.005x - 0.60.9998
50
100
150
200
250
50 100 150 200 250
(mg/dL)
Hita
chi ,
Hex
okin
ase
G6P
DH
(B
)
Hitachi , Hexokinase G6PDH (A)
y =R =
1.010x + 0.90.9996
50
100
150
200
250
50 100 150 200 250
(mg/dL)
Roc
he ,
Hex
okin
ase
G6P
DH
(I)
Hitachi , Hexokinase G6PDH (A)
y =R =
1.022x - 0.50.9999
50
100
150
200
250
50 100 150 200 250
(mg/dL)
Fuj
ifilm
,D
ry-c
hem
istr
y (A
)
Hitachi , Hexokinase G6PDH (A)
y =R =
1.005x + 1.40.9996
50
100
150
200
250
50 100 150 200 250
(mg/dL)
Hita
chi ,
Gly
coki
nase
(A
)
Hitachi , Hexokinase G6PDH (A)
y =R =
1.034x - 0.10.9998
50
100
150
200
250
50 100 150 200 250
(mg/dL)
Ort
ho ,
Dry
-che
mis
try
(B)
Hitachi , Hexokinase G6PDH (A)
y =R =
0.989x + 0.60.9988
50
100
150
200
250
50 100 150 200 250
(mg/dL)
Bec
kman
Cou
lter
,H
exok
inas
e G
6PD
H (
K)
Hitachi , Hexokinase G6PDH (A)
y =R =
1.023x - 0.90.9997
50
100
150
200
250
50 100 150 200 250
(mg/dL)
Hita
chi ,
Hex
okin
ase
G6P
DH
(D
)
Hitachi , Hexokinase G6PDH (A)
y =R =
1.042x + 0.40.9993
Suppl. fig. 1b. Assessment of MacRM commutability by twin plot analysis (Glucose)
: Patient sera (n=30) : NIST SRM917c : MacRM-001
50
100
150
200
250
50 100 150 200 250
(mg/dL)
Hita
chi ,
Gly
coki
nase
(B
)
Hitachi , Hexokinase G6PDH (A)
y =R =
1.015x + 1.40.9980
50
100
150
200
250
50 100 150 200 250
(mg/dL)
Hita
chi ,
Hex
okin
ase
G6P
DH
(E
)
Hitachi , Hexokinase G6PDH (A)
y =R =
1.053x + 0.00.9996
50
100
150
200
250
50 100 150 200 250
(mg/dL)
Hita
chi ,
Hex
okin
ase
G6P
DH
(H
)
Hitachi , Hexokinase G6PDH (A)
y =R =
1.038x + 0.00.9997
50
100
150
200
250
50 100 150 200 250
(mg/dL)
Hita
chi ,
Hex
okin
ase
G6P
DH
(J)
Hitachi , Hexokinase G6PDH (A)
y =R =
1.038x - 0.20.9998
50
100
150
200
250
50 100 150 200 250
(mg/dL)
Hita
chi ,
Hex
okin
ase
G6P
DH
(L
)
Hitachi , Hexokinase G6PDH (A)
y =R =
1.043x - 0.40.9998
50
100
150
200
250
50 100 150 200 250
(mg/dL)
Hita
chi ,
Hex
okin
ase
G6P
DH
(G
)
Hitachi , Hexokinase G6PDH (A)
y =R =
1.018x + 0.70.9996
Suppl. fig. 2a. Assessment of MacRM commutability by twin plot analysis (UA)
: Patient sera (n=30) : NIST SRM913b : MacRM-001
0
2
4
6
8
10
12
14
0 2 4 6 8 10 12 14
(mg/dL)
Hita
chi ,
Uri
case
-per
oxid
ase
(E)
Hitachi , Uricase-peroxidase (A)
y =R =
0.996x + 0.140.9999
0
2
4
6
8
10
12
14
0 2 4 6 8 10 12 14
(mg/dL)
Hita
chi ,
Uri
case
-per
oxid
ase
(F)
Hitachi , Uricase-peroxidase (A)
y =R =
0.990x + 0.100.9999
0
2
4
6
8
10
12
14
0 2 4 6 8 10 12 14
(mg/dL)
JEO
L ,
Uri
case
-per
oxid
ase
(E)
Hitachi , Uricase-peroxidase (A)
y =R =
0.988x + 0.090.9999
0
2
4
6
8
10
12
14
0 2 4 6 8 10 12 14
(mg/dL)
Hita
chi ,
Uri
case
-per
oxid
ase
(C)
Hitachi , Uricase-peroxidase (A)
y =R =
1.010x + 0.100.9998
0
2
4
6
8
10
12
14
0 2 4 6 8 10 12 14
(mg/dL)
Roc
he ,
Uri
case
-per
oxid
ase
(J)
Hitachi , Uricase-peroxidase (A)
y =R =
0.994x + 0.030.9996
0
2
4
6
8
10
12
14
0 2 4 6 8 10 12 14
(mg/dL)
Fuj
ifilm
,D
ry-c
hem
istr
y (A
)
Hitachi , Uricase-peroxidase (A)
y =R =
0.997x + 0.180.9988
0
2
4
6
8
10
12
14
0 2 4 6 8 10 12 14
(mg/dL)
Hita
chi ,
Uri
case
-per
oxid
ase
(B)
Hitachi , Uricase-peroxidase (A)
y =R =
0.979x + 0.060.9999
0
2
4
6
8
10
12
14
0 2 4 6 8 10 12 14
(mg/dL)
Sie
men
s ,
Uri
case
UV
Hitachi , Uricase-peroxidase (A)
y =R =
0.977x - 0.090.9995
0
2
4
6
8
10
12
14
0 2 4 6 8 10 12 14
(mg/dL)
Hita
chi ,
Uri
case
-per
oxid
ase
(D)
Hitachi , Uricase-peroxidase (A)
y =R =
0.985x + 0.100.9998
0
2
4
6
8
10
12
14
0 2 4 6 8 10 12 14
(mg/dL)
Ort
ho ,
Dry
-che
mis
try
(B)
Hitachi , Uricase-peroxidase (A)
y =R =
0.979x + 0.110.9996
0
2
4
6
8
10
12
14
0 2 4 6 8 10 12 14
(mg/dL)
Bec
kman
Cou
lter
,U
rica
se-p
erox
idas
e (M
)
Hitachi , Uricase-peroxidase (A)
y =R =
0.968x + 0.120.9980
0
2
4
6
8
10
12
14
0 2 4 6 8 10 12 14
(mg/dL)
Hita
chi ,
Uri
case
-per
oxid
ase
(G)
Hitachi , Uricase-peroxidase (A)
y =R =
1.003x + 0.090.9995
Suppl. fig. 2b. Assessment of MacRM commutability by twin plot analysis (UA)
: Patient sera (n=30) : NIST SRM913b : MacRM-001
0
2
4
6
8
10
12
14
0 2 4 6 8 10 12 14
(mg/dL)
Hita
chi ,
Uri
case
-per
oxid
ase
(H)
Hitachi , Uricase-peroxidase (A)
y =R =
0.992x + 0.050.9999
0
2
4
6
8
10
12
14
0 2 4 6 8 10 12 14
(mg/dL)
Hita
chi ,
Uri
case
-per
oxid
ase
(I)
Hitachi , Uricase-peroxidase (A)
y =R =
1.000x + 0.090.9998
0
2
4
6
8
10
12
14
0 2 4 6 8 10 12 14
(mg/dL)
Hita
chi ,
Uri
case
-per
oxid
ase
(L)
Hitachi , Uricase-peroxidase (A)
y =R =
0.995x + 0.100.9998
0
2
4
6
8
10
12
14
0 2 4 6 8 10 12 14
(mg/dL)
Hita
chi ,
Uri
case
-per
oxid
ase
(B)
Hitachi , Uricase-peroxidase (A)
y =R =
0.987x +0.030.9999
0
2
4
6
8
10
12
14
0 2 4 6 8 10 12 14
(mg/dL)
Hita
chi ,
Uri
case
-per
oxid
ase
(K)
Hitachi , Uricase-peroxidase (A)
y =R =
1.008x + 0.050.9998
Suppl. fig. 3a. Assessment of MacRM commutability by twin plot analysis (UN)
: Patient sera (n=27) : NIST SRM912a : MacRM-001
0
10
20
30
40
50
60
0 10 20 30 40 50 60
(mg/dL)
JEO
L ,
Ure
ase-
GL
DH
-IC
DH
(A
)
Hitachi , Urease-GLDH (A)
y =R =
1.011x - 0.10.9999
0
10
20
30
40
50
60
0 10 20 30 40 50 60
(mg/dL)
Sie
men
s ,
Ure
ase-
GL
DH
(H
)
Hitachi , Urease-GLDH (A)
y =R =
1.032x + 0.10.9997
0
10
20
30
40
50
60
0 10 20 30 40 50 60
(mg/dL)
Fuj
ifilm
,D
ry-c
hem
istr
y (A
)
Hitachi , Urease-GLDH (A)
y =R =
1.016x + 0.20.9998
0
10
20
30
40
50
60
0 10 20 30 40 50 60
(mg/dL)
Hita
chi ,
Ure
ase-
GL
DH
(B
)
Hitachi , Urease-GLDH (A)
y =R =
1.032x +0.00.9998
0
10
20
30
40
50
60
0 10 20 30 40 50 60
(mg/dL)
Hita
chi ,
Ure
ase-
GL
DH
-IC
DH
(A
)
Hitachi , Urease-GLDH (A)
y =R =
1.033x - 0.10.9998
0
10
20
30
40
50
60
0 10 20 30 40 50 60
(mg/dL)
Hita
chi ,
Ure
ase-
GL
DH
(E
)
Hitachi , Urease-GLDH (A)
y =R =
1.028x + 0.40.9990
0
10
20
30
40
50
60
0 10 20 30 40 50 60
(mg/dL)
Hita
chi ,
Ure
ase-
GL
DH
-IC
DH
(B
)
Hitachi , Urease-GLDH (A)
y =R =
1.028x + 0.20.9997
0
10
20
30
40
50
60
0 10 20 30 40 50 60
(mg/dL)
Roc
he ,
Ure
ase-
GL
DH
(K
)
Hitachi , Urease-GLDH (A)
y =R =
0.966x + 0.50.9997
0
10
20
30
40
50
60
0 10 20 30 40 50 60
(mg/dL)
Bec
kman
Cou
lter
,U
reas
e-G
LD
H (
J)
Hitachi , Urease-GLDH (A)
y =R =
1.022x - 0.70.9997
0
10
20
30
40
50
60
0 10 20 30 40 50 60
(mg/dL)
Ort
ho ,
Dry
-che
mis
try
(B)
Hitachi , Urease-GLDH (A)
y =R =
0.992x + 0.80.9984
0
10
20
30
40
50
60
0 10 20 30 40 50 60
(mg/dL)
Hita
chi ,
Ure
ase-
leuc
in d
ehyd
roge
nase
(A
)
Hitachi , Urease-GLDH (A)
y =R =
1.030x + 0.10.9997
0
10
20
30
40
50
60
0 10 20 30 40 50 60
(mg/dL)
Hita
chi ,
Ure
ase-
GL
DH
(C
)
Hitachi , Urease-GLDH (A)
y =R =
1.033x + 0.10.9998
Suppl. fig. 3b. Assessment of MacRM commutability by twin plot analysis (UN)
: Patient sera (n=27) : NIST SRM912a : MacRM-001
0
10
20
30
40
50
60
0 10 20 30 40 50 60
(mg/dL)
Hita
chi ,
Ure
ase-
GL
DH
(D
)
Hitachi , Urease-GLDH (A)
y =R =
1.022x - 0.10.9999
0
10
20
30
40
50
60
0 10 20 30 40 50 60
(mg/dL)
Hita
chi ,
Ure
ase-
GL
DH
-IC
DH
(C
)
Hitachi , Urease-GLDH (A)
y =R =
1.034x - 0.30.9998
0
10
20
30
40
50
60
0 10 20 30 40 50 60
(mg/dL)
Hita
chi ,
Ure
ase-
leuc
in d
ehyd
roge
nase
(C
)
Hitachi , Urease-GLDH (A)
y =R =
1.013x + 0.10.9996
0
10
20
30
40
50
60
0 10 20 30 40 50 60
(mg/dL)
Hita
chi ,
Ure
ase-
GL
DH
-IC
DH
(D
)
Hitachi , Urease-GLDH (A)
y =R =
1.021x + 0.00.9999
0
10
20
30
40
50
60
0 10 20 30 40 50 60
(mg/dL)
Hita
chi ,
Ure
ase-
GL
DH
-IC
DH
(E
)
Hitachi , Urease-GLDH (A)
y =R =
1.019x - 0.30.9998
0
10
20
30
40
50
60
0 10 20 30 40 50 60
(mg/dL)
Hita
chi ,
Ure
ase-
GL
DH
-IC
DH
(F
)
Hitachi , Urease-GLDH (A)
y =R =
1.014x + 0.30.9997
0
10
20
30
40
50
60
0 10 20 30 40 50 60
(mg/dL)
Hita
chi ,
Ure
ase-
GL
DH
-IC
DH
(G
)
Hitachi , Urease-GLDH (A)
y =R =
1.023x + 0.20.9997
0
10
20
30
40
50
60
0 10 20 30 40 50 60
(mg/dL)
Hita
chi ,
Ure
ase-
GL
DH
(I)
Hitachi , Urease-GLDH (A)
y =R =
1.016x + 0.30.9998
0
10
20
30
40
50
60
0 10 20 30 40 50 60
(mg/dL)
Hita
chi ,
Ure
ase-
leuc
in d
ehyd
roge
nase
(B
)
Hitachi , Urease-GLDH (A)
y =R =
1.012x + 0.00.9998
0
10
20
30
40
50
60
0 10 20 30 40 50 60
(mg/dL)
Hita
chi ,
Ure
ase-
GL
DH
(G
)
Hitachi , Urease-GLDH (A)
y =R =
1.016x + 0.30.9994
0
10
20
30
40
50
60
0 10 20 30 40 50 60
(mg/dL)
Hita
chi ,
Ure
ase-
leuc
in d
ehyd
roge
nase
(D
)
Hitachi , Urease-GLDH (A)
y =R =
1.001x - 0.10.9998
0
10
20
30
40
50
60
0 10 20 30 40 50 60
(mg/dL)
Hita
chi ,
Ure
ase-
GL
DH
-IC
DH
(H
)
Hitachi , Urease-GLDH (A)
y =R =
1.035x - 0.10.9999
Suppl. fig. 3c. Assessment of MacRM commutability by twin plot analysis (UN)
: Patient sera (n=27) : NIST SRM912a : MacRM-001
0
10
20
30
40
50
60
0 10 20 30 40 50 60
(mg/dL)
Hita
chi ,
Ure
ase-
GL
DH
-IC
DH
(I)
Hitachi , Urease-GLDH (A)
y =R =
1.037x - 0.10.9998
0
10
20
30
40
50
60
0 10 20 30 40 50 60
(mg/dL)
Hita
chi ,
Ure
ase-
GL
DH
(F
)
Hitachi , Urease-GLDH (A)
y =R =
1.025x - 0.20.9999
0
10
20
30
40
50
60
0 10 20 30 40 50 60
(mg/dL)
Hita
chi ,
Ure
ase-
GL
DH
-IC
DH
(F
)
Hitachi , Urease-GLDH (A)
y =R =
1.028x + 0.20.9996
0
10
20
30
40
50
60
0 10 20 30 40 50 60
(mg/dL)
Hita
chi ,
Ure
ase-
GL
DH
-IC
DH
(J)
Hitachi , Urease-GLDH (A)
y =R =
1.004x + 0.30.9992
Suppl. fig. 4a. Assessment of MacRM commutability by twin plot analysis (Cre)
: Patient sera (n=30) : NIST SRM914a : MacRM-001
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
JEO
L ,
Enz
ymat
ic m
etho
d (S
)
Hitachi , Enzymatic method (A)
y =R =
1.003x - 0.050.9999
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (C
)
Hitachi , Enzymatic method (A)
y =R =
0.999x - 0.020.9999
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (B
)
Hitachi , Enzymatic method (A)
y =R =
1.006x - 0.010.9999
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Sie
men
s ,
Jaff
e ra
te a
ssay
(A
)
Hitachi , Enzymatic method (A)
y =R =
1.026x + 0.100.9986
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Roc
he ,
Enz
ymat
ic m
etho
d (R
)
Hitachi , Enzymatic method (A)
y =R =
0.983x + 0.030.9999
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Fuj
ifilm
,D
ry-c
hem
istr
y (A
)
Hitachi , Enzymatic method (A)
y =R =
1.016x - 0.030.9994
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (E
)
Hitachi , Enzymatic method (A)
y =R =
1.031x - 0.020.9999
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (F
)
Hitachi , Enzymatic method (A)
y =R =
0.986x + 0.010.9999
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Ort
ho ,
Dry
-che
mis
try
(B)
Hitachi , Enzymatic method (A)
y =R =
1.055x - 0.040.9996
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Bec
kman
Cou
lter
,Ja
ffe
rate
ass
ay (
B)
Hitachi , Enzymatic method (A)
y =R =
0.988x + 0.010.9997
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (D
)
Hitachi , Enzymatic method (A)
y =R =
1.034x - 0.030.9999
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (G
)
Hitachi , Enzymatic method (A)
y =R =
0.985x + 0.050.9999
Suppl. fig. 4b. Assessment of MacRM commutability by twin plot analysis (Cre)
: Patient sera (n=30) : NIST SRM914a : MacRM-001
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (N
)
Hitachi , Enzymatic method (A)
y =R =
0.998x + 0.050.9999
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (O
)
Hitachi , Enzymatic method (A)
y =R =
1.022x + 0.060.9998
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (H
)
Hitachi , Enzymatic method (A)
y =R =
0.989x + 0.030.9999
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (I
)
Hitachi , Enzymatic method (A)
y =R =
1.017x - 0.040.9999
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (K
)
Hitachi , Enzymatic method (A)
y =R =
0.994x + 0.020.9999
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (L
)
Hitachi , Enzymatic method (A)
y =R =
1.004x - 0.040.9999
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (Q
)
Hitachi , Enzymatic method (A)
y =R =
1.004x + 0.030.9999
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (G
)
Hitachi , Enzymatic method (A)
y =R =
0.989x + 0.020.9999
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (M
)
Hitachi , Enzymatic method (A)
y =R =
1.007x - 0.030.9999
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (J
)
Hitachi , Enzymatic method (A)
y =R =
1.007x + 0.030.9999
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (P
)
Hitachi , Enzymatic method (A)
y =R =
1.005x + 0.060.9999
Suppl. fig. 5. Assessment of MacRM commutability by twin plot analysis (TB)
: Patient sera (n=30) : MacRM-001
0
5
10
15
0 5 10 15
(mg/dL)
Hita
chi ,
Bili
rubi
n ox
idas
e (A
)
Hitachi , Vanadic oxidation
y =R =
1.077x - 0.010.9999
0
5
10
15
0 5 10 15
(mg/dL)
Sie
men
s ,
Dia
zo (
A)
Hitachi , Vanadic oxidation
y =R =
1.100x -0.100.9997
0
5
10
15
0 5 10 15
(mg/dL)
Hita
chi ,
Nitr
ite o
xida
tion
Hitachi , Vanadic oxidation
y =R =
1.061x + 0.090.9999
0
5
10
15
0 5 10 15
(mg/dL)
Fuj
ifilm
,D
ry-c
hem
istr
y (A
)
Hitachi , Vanadic oxidation
y =R =
1.085x + 0.100.9992
0
5
10
15
0 5 10 15
(mg/dL)
Hita
chi ,
Bili
rubi
n ox
idas
e (B
)
Hitachi , Vanadic oxidation
y =R =
1.062x + 0.040.9999
0
5
10
15
0 5 10 15
(mg/dL)
Hita
chi ,
Bili
rubi
n ox
idas
e (C
)
Hitachi , Vanadic oxidation
y =R =
1.056x + 0.070.9998
0
5
10
15
0 5 10 15
(mg/dL)
Hita
chi ,
Bili
rubi
n ox
idas
e (E
)
Hitachi , Vanadic oxidation
y =R =
1.068x +0.100.9999
0
5
10
15
0 5 10 15
(mg/dL)
Hita
chi ,
Bili
rubi
n ox
idas
e (F
)
Hitachi , Vanadic oxidation
y =R =
1.085x + 0.100.9998
0
5
10
15
0 5 10 15
(mg/dL)
Roc
he ,
Dia
zo (
B)
Hitachi , Vanadic oxidation
y =R =
1.150x - 0.160.9998
0
5
10
15
0 5 10 15
(mg/dL)
Ort
ho ,
Dry
-che
mis
try
(B)
Hitachi , Vanadic oxidation
y =R =
1.083x - 0.140.9973
0
5
10
15
0 5 10 15
(mg/dL)
Hita
chi ,
Bili
rubi
n ox
idas
e (D
)
Hitachi , Vanadic oxidation
y =R =
1.036x + 0.030.9999
0
5
10
15
0 5 10 15
(mg/dL)
Hita
chi ,
Dia
zo (
C)
Hitachi , Vanadic oxidation
y =R =
1.147x - 0.090.9998
Suppl. fig. 6. Assessment of MacRM commutability by twin plot analysis (Fe)
: Patient sera (n=30) : NIST SRM937: MacRM-001
0
50
100
150
200
0 50 100 150 200
(μg/dL)
Hita
chi ,
Bat
hoph
enan
thro
line
(A)
Hitachi , Nitroso-PSAP (A)
y =R =
1.036x - 0.40.9992
0
50
100
150
200
0 50 100 150 200
(μg/dL)
Sie
men
s ,
Fer
ene
Hitachi , Nitroso-PSAP (A)
y =R =
0.989x + 0.50.9993
0
50
100
150
200
0 50 100 150 200
(μg/dL)
JEO
L ,
Nitr
oso-
PS
AP
(A
)
Hitachi , Nitroso-PSAP (A)
y =R =
1.016x - 2.00.9994
0
50
100
150
200
0 50 100 150 200
(μg/dL)
Ort
ho ,
Dry
-che
mis
try
Hitachi , Nitroso-PSAP (A)
y =R =
1.063x - 0.80.9970
0
50
100
150
200
0 50 100 150 200
(μg/dL)
Hita
chi ,
Bat
hoph
enan
thro
line
(B)
Hitachi , Nitroso-PSAP (A)
y =R =
1.037x - 2.20.9990
0
50
100
150
200
0 50 100 150 200
(μg/dL)
Hita
chi ,
Nitr
oso-
PS
AP
(C
)
Hitachi , Nitroso-PSAP (A)
y =R =
1.021x - 2.00.9990
0
50
100
150
200
0 50 100 150 200
(μg/dL)
Hita
chi ,
Nitr
oso-
PS
AP
(E
)
Hitachi , Nitroso-PSAP (A)
y =R =
1.004x - 1.50.9994
0
50
100
150
200
0 50 100 150 200
(μg/dL)
Hita
chi ,
Nitr
oso-
PS
AP
(A
)
Hitachi , Nitroso-PSAP (A)
y =R =
1.001x - 0.50.9999
0
50
100
150
200
0 50 100 150 200
(μg/dL)
Bec
kman
Cou
lter
,F
erro
zine
Hitachi , Nitroso-PSAP (A)
y =R =
1.038x - 1.70.9993
0
50
100
150
200
0 50 100 150 200
(μg/dL)
Hita
chi ,
Nitr
oso-
PS
AP
(B
)
Hitachi , Nitroso-PSAP (A)
y =R =
1.001x - 1.00.9999
0
50
100
150
200
0 50 100 150 200
(μg/dL)
Hita
chi ,
Nitr
oso-
PS
AP
(D
)
Hitachi , Nitroso-PSAP (A)
y =R =
1.007x - 0.90.9999
Suppl. fig. 7. Assessment of MacRM commutability by twin plot analysis (Na)
: Patient sera (n=30) : JCCRM 111-6: MacRM-001
120
130
140
150
160
120 130 140 150 160
(mmol/L)
JEO
L ,
Ion
sele
ctiv
e el
ectr
ode
(B)
Hitachi , Ion selective electrode (A)
y =R =
0.989x - 1.00.9961
120
130
140
150
160
120 130 140 150 160
(mmol/L)
Sie
men
s ,
Ion
sele
ctiv
e el
ectr
ode
(D)
Hitachi , Ion selective electrode (A)
y =R =
0.985x + 1.80.9946
120
130
140
150
160
120 130 140 150 160
(mmol/L)
Bec
kman
Cou
lter
,Io
n se
lect
ive
elec
trod
e (F
)
Hitachi , Ion selective electrode (A)
y =R =
0.876x + 13.90.9941
120
130
140
150
160
120 130 140 150 160
(mmol/L)
Fuj
ifilm
,D
ry-c
hem
istr
y (A
)
Hitachi , Ion selective electrode (A)
y =R =
0.838x + 24.60.9573
120
130
140
150
160
120 130 140 150 160
(mmol/L)
Tos
hiba
,Io
n se
lect
ive
elec
trod
e (C
)
Hitachi , Ion selective electrode (A)
y =R =
1.007x - 2.50.9958
120
130
140
150
160
120 130 140 150 160
(mmol/L)
Hita
chi ,
Ion
sele
ctiv
e el
ectr
ode
(A)
Hitachi , Ion selective electrode (A)
y =R =
0.978x + 1.70.9956
120
130
140
150
160
120 130 140 150 160
(mmol/L)
Hita
chi ,
Ion
sele
ctiv
e el
ectr
ode
(A)
Hitachi , Ion selective electrode (A)
y =R =
0.994x + 0.00.9975
120
130
140
150
160
120 130 140 150 160
(mmol/L)
Bec
kman
Cou
lter
,Io
n se
lect
ive
elec
trod
e (E
)
Hitachi , Ion selective electrode (A)
y =R =
0.994x - 0.30.9970
120
130
140
150
160
120 130 140 150 160
(mmol/L)
Ort
ho ,
Dry
-che
mis
try
(B)
Hitachi , Ion selective electrode (A)
y =R =
0.917x + 11.80.9464
Suppl. fig. 8. Assessment of MacRM commutability by twin plot analysis (K)
: Patient sera (n=30) : JCCRM 111-6: MacRM-001
1
2
3
4
5
6
1 2 3 4 5 6
(mmol/L)
Bec
kman
Cou
lter
,Io
n se
lect
ive
elec
trod
e (F
)
Hitachi , Ion selective electrode (A)
y =R =
0.982x + 0.040.9994
1
2
3
4
5
6
1 2 3 4 5 6
(mmol/L)
Fuj
ifilm
,D
ry-c
hem
istr
y (A
)
Hitachi , Ion selective electrode (A)
y =R =
1.084x - 0.340.9980
1
2
3
4
5
6
1 2 3 4 5 6
(mmol/L)
JEO
L ,
Ion
sele
ctiv
e el
ectr
ode
(B)
Hitachi , Ion selective electrode (A)
y =R =
1.034x - 0.060.9993
1
2
3
4
5
6
1 2 3 4 5 6
(mmol/L)
Sie
men
s ,
Ion
sele
ctiv
e el
ectr
ode
(D)
Hitachi , Ion selective electrode (A)
y =R =
1.035x - 0.150.9998
1
2
3
4
5
6
1 2 3 4 5 6
(mmol/L)
Tos
hiba
,Io
n se
lect
ive
elec
trod
e (C
)
Hitachi , Ion selective electrode (A)
y =R =
1.008x - 0.060.9998
1
2
3
4
5
6
1 2 3 4 5 6
(mmol/L)
Hita
chi ,
Ion
sele
ctiv
e el
ectr
ode
(A)
Hitachi , Ion selective electrode (A)
y =R =
0.990x + 0.040.9998
1
2
3
4
5
6
1 2 3 4 5 6
(mmol/L)
Hita
chi ,
Ion
sele
ctiv
e el
ectr
ode
(A)
Hitachi , Ion selective electrode (A)
y =R =
0.991x + 0.060.9999
1
2
3
4
5
6
1 2 3 4 5 6
(mmol/L)
Bec
kman
Cou
lter
,Io
n se
lect
ive
elec
trod
e (E
)
Hitachi , Ion selective electrode (A)
y =R =
0.966x + 0.130.9998
1
2
3
4
5
6
1 2 3 4 5 6
(mmol/L)
Ort
ho ,
Dry
-che
mis
try
(B)
Hitachi , Ion selective electrode (A)
y =R =
1.055x - 0.150.9987
Suppl. fig. 9. Assessment of MacRM commutability by twin plot analysis (Cl)
: Patient sera (n=30) : JCCRM 111-6: MacRM-001
80
90
100
110
120
80 90 100 110 120
(mmol/L)
JEO
L ,
Ion
sele
ctiv
e el
ectr
ode
(B)
Hitachi , Ion selective electrode (A)
y =R =
0.962x + 0.60.9747
80
90
100
110
120
80 90 100 110 120
(mmol/L)
Sie
men
s ,
Ion
sele
ctiv
e el
ectr
ode
(D)
Hitachi , Ion selective electrode (A)
y =R =
0.863x + 11.70.9730
80
90
100
110
120
80 90 100 110 120
(mmol/L)
Bec
kman
Cou
lter
,Io
n se
lect
ive
elec
trod
e (F
)
Hitachi , Ion selective electrode (A)
y =R =
0.916x + 7.20.9616
80
90
100
110
120
80 90 100 110 120
(mmol/L)
Fuj
ifilm
,D
ry-c
hem
istr
y (A
)
Hitachi , Ion selective electrode (A)
y =R =
0.904x + 7.90.9180
80
90
100
110
120
80 90 100 110 120
(mmol/L)
Tos
hiba
,Io
n se
lect
ive
elec
trod
e (C
)
Hitachi , Ion selective electrode (A)
y =R =
0.917x + 7.20.9659
80
90
100
110
120
80 90 100 110 120
(mmol/L)
Hita
chi ,
Ion
sele
ctiv
e el
ectr
ode
(A)
Hitachi , Ion selective electrode (A)
y =R =
0.953x + 3.70.9830
80
90
100
110
120
80 90 100 110 120
(mmol/L)
Hita
chi ,
Ion
sele
ctiv
e el
ectr
ode
(A)
Hitachi , Ion selective electrode (A)
y =R =
0.951x + 5.20.9834
80
90
100
110
120
80 90 100 110 120
(mmol/L)
Bec
kman
Cou
lter
,Io
n se
lect
ive
elec
trod
e (E
)
Hitachi , Ion selective electrode (A)
y =R =
0.878x + 10.70.9579
80
90
100
110
120
80 90 100 110 120
(mmol/L)
Ort
ho ,
Dry
-che
mis
try
(B)
Hitachi , Ion selective electrode (A)
y =R =
0.938x + 3.70.9463
Suppl. fig. 10a. Assessment of MacRM commutability by twin plot analysis (Ca)
: Patient sera (n=30) : NIST SRM915b: MacRM-001
6
7
8
9
10
6 7 8 9 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (A
)
Hitachi , Arsenazo-III (A)
y =R =
0.943x + 0.620.9969
6
7
8
9
10
6 7 8 9 10
(mg/dL)
Sie
men
s ,
o-C
reso
lpht
hale
in c
ompl
exon
e (A
)
Hitachi , Arsenazo-III (A)
y =R =
0.865x + 1.270.9941
6
7
8
9
10
6 7 8 9 10
(mg/dL)
Bec
kman
Cou
lter
,o-
Cre
solp
htha
lein
com
plex
one
(C)
Hitachi , Arsenazo-III (A)
y =R =
0.964x + 0.330.9915
6
7
8
9
10
6 7 8 9 10
(mg/dL)
Fuj
ifilm
,D
ry-c
hem
istr
y (A
)
Hitachi , Arsenazo-III (A)
y =R =
1.016x - 0.270.9946
6
7
8
9
10
6 7 8 9 10
(mg/dL)
Tos
hiba
,o-
Cre
solp
htha
lein
com
plex
one
(E)
Hitachi , Arsenazo-III (A)
y =R =
1.030x - 0.170.9969
6
7
8
9
10
6 7 8 9 10
(mg/dL)
JEO
L ,
Met
hyl x
ylen
ol b
lue
(A)
Hitachi , Arsenazo-III (A)
y =R =
1.022x - 0.510.9968
6
7
8
9
10
6 7 8 9 10
(mg/dL)
Hita
chi ,
Ars
enaz
o-II
I (B
)
Hitachi , Arsenazo-III (A)
y =R =
0.970x + 0.560.9952
6
7
8
9
10
6 7 8 9 10
(mg/dL)
Hita
chi ,
Met
hyl x
ylen
ol b
lue
(A)
Hitachi , Arsenazo-III (A)
y =R =
0.996x - 0.130.9967
6
7
8
9
10
6 7 8 9 10
(mg/dL)
Bec
kman
Cou
lter
,Io
n se
lect
ive
elec
trod
e
Hitachi , Arsenazo-III (A)
y =R =
0.978x + 0.130.9903
6
7
8
9
10
6 7 8 9 10
(mg/dL)
Ort
ho ,
Dry
-che
mis
try
(B)
Hitachi , Arsenazo-III (A)
y =R =
1.040x - 0.280.9932
6
7
8
9
10
6 7 8 9 10
(mg/dL)
Hita
chi ,
Chl
orop
hosp
hona
zo-I
II (
A)
Hitachi , Arsenazo-III (A)
y =R =
0.998x + 0.270.9978
6
7
8
9
10
6 7 8 9 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (B
)
Hitachi , Arsenazo-III (A)
y =R =
1.016x + 0.140.9963
Suppl. fig. 10b. Assessment of MacRM commutability by twin plot analysis (Ca)
: Patient sera (n=30)
6
7
8
9
10
6 7 8 9 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (C
)
Hitachi , Arsenazo-III (A)
y =R =
0.996x - 0.020.9966
6
7
8
9
10
6 7 8 9 10
(mg/dL)
Hita
chi ,
Met
hyl x
ylen
ol b
lue
(B)
Hitachi , Arsenazo-III (A)
y =R =
0.979x + 0.040.9951
6
7
8
9
10
6 7 8 9 10
(mg/dL)
Hita
chi ,
Chl
orop
hosp
hona
zo-I
II (
B)
Hitachi , Arsenazo-III (A)
y =R =
1.007x - 0.460.9868
6
7
8
9
10
6 7 8 9 10
(mg/dL)
Hita
chi ,
o-C
reso
lpht
hale
in c
ompl
exon
e (D
)
Hitachi , Arsenazo-III (A)
y =R =
0.972x + 0.350.9949
: NIST SRM915b: MacRM-001
6
7
8
9
10
6 7 8 9 10
(mg/dL)
Hita
chi ,
o-C
reso
lpht
hale
in c
ompl
exon
e (B
)
Hitachi , Arsenazo-III (A)
y =R =
1.059x - 0.330.9966
Suppl. fig. 11a. Assessment of MacRM commutability by twin plot analysis (IP)
: Patient sera (n=30) : NIST SRM200b: MacRM-001
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (B
)
Hitachi , Enzymatic method (A)
y =R =
0.997x - 0.080.9998
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Sie
men
s ,
Mol
ybda
te U
V (
B)
Hitachi , Enzymatic method (A)
y =R =
0.993x - 0.050.9996
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Hita
chi ,
Mol
ybda
te b
lue
Hitachi , Enzymatic method (A)
y =R =
0.962x + 0.150.9988
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Fuj
ifilm
,D
ry-c
hem
istr
y (A
)
Hitachi , Enzymatic method (A)
y =R =
1.044x - 0.020.9989
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
JEO
L ,
Enz
ymat
ic m
etho
d (D
)
Hitachi , Enzymatic method (A)
y =R =
1.035x - 0.080.9999
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (C
)
Hitachi , Enzymatic method (A)
y =R =
0.999x - 0.040.9997
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (D
)
Hitachi , Enzymatic method (A)
y =R =
1.009x - 0.060.9998
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (E
)
Hitachi , Enzymatic method (A)
y =R =
0.995x - 0.080.9994
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Bec
kman
Cou
lter
,M
olyb
date
UV
(C
)
Hitachi , Enzymatic method (A)
y =R =
0.966x - 0.030.9993
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Ort
ho ,
Dry
-che
mis
try
(B)
Hitachi , Enzymatic method (A)
y =R =
1.044x + 0.250.9989
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Hita
chi ,
Mol
ybda
te U
V (
A)
Hitachi , Enzymatic method (A)
y =R =
0.998x - 0.080.9994
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (F
)
Hitachi , Enzymatic method (A)
y =R =
0.993x + 0.000.9999
Suppl. fig. 11b. Assessment of MacRM commutability by twin plot analysis (IP)
: Patient sera (n=30) : NIST SRM200b: MacRM-001
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (G
)
Hitachi , Enzymatic method (A)
y =R =
0.992x + 0.060.9993
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (H
)
Hitachi , Enzymatic method (A)
y =R =
0.984x + 0.040.9998
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (J
)
Hitachi , Enzymatic method (A)
y =R =
0.981x + 0.000.9998
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (A
)
Hitachi , Enzymatic method (A)
y =R =
0.982x + 0.020.9999
0
2
4
6
8
10
0 2 4 6 8 10
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (I
)
Hitachi , Enzymatic method (A)
y =R =
0.979x + 0.030.9998
Suppl. fig. 12a. Assessment of MacRM commutability by twin plot analysis (Mg)
: Patient sera (n=30) : NIST SRM929a: MacRM-001
0
1
2
3
4
5
6
0 1 2 3 4 5 6
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (B
)
y =R =
1.050x - 0.030.9990
Hitachi , Enzymatic method (A)
0
1
2
3
4
5
6
0 1 2 3 4 5 6
(mg/dL)
Sie
men
s ,
Enz
ymat
ic m
etho
d (F
)
y =R =
1.040x - 0.100.9989
Hitachi , Enzymatic method (A)
0
1
2
3
4
5
6
0 1 2 3 4 5 6
(mg/dL)
JEO
L ,
Enz
ymat
ic m
etho
d (C
)
y =R =
1.009x + 0.000.9991
Hitachi , Enzymatic method (A)
0
1
2
3
4
5
6
0 1 2 3 4 5 6
(mg/dL)
Fuj
ifilm
,D
ry-c
hem
istr
y (A
)
y =R =
1.045x - 0.120.9847
Hitachi , Enzymatic method (A)
0
1
2
3
4
5
6
0 1 2 3 4 5 6
(mg/dL)
Tos
hiba
,E
nzym
atic
met
hod
(G)
y =R =
1.009x - 0.040.9996
Hitachi , Enzymatic method (A)
0
1
2
3
4
5
6
0 1 2 3 4 5 6
(mg/dL)
Hita
chi ,
Xyl
idyl
blu
e (A
)
y =R =
0.986x + 0.110.9980
Hitachi , Enzymatic method (A)
0
1
2
3
4
5
6
0 1 2 3 4 5 6
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (D
)
y =R =
1.016x - 0.110.9993
Hitachi , Enzymatic method (A)
0
1
2
3
4
5
6
0 1 2 3 4 5 6
(mg/dL)
Hita
chi ,
Xyl
idyl
blu
e (B
)
y =R =
0.987x + 0.060.9988
Hitachi , Enzymatic method (A)
0
1
2
3
4
5
6
0 1 2 3 4 5 6
(mg/dL)
Bec
kman
Cou
lter
,X
ylid
yl b
lue
(D)
y =R =
0.983x - 0.010.9984
Hitachi , Enzymatic method (A)
0
1
2
3
4
5
6
0 1 2 3 4 5 6
(mg/dL)
Ort
ho ,
Dry
-che
mis
try
(B)
y =R =
1.063x - 0.190.9974
Hitachi , Enzymatic method (A)
0
1
2
3
4
5
6
0 1 2 3 4 5 6
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (C
)
y =R =
1.019x - 0.040.9993
Hitachi , Enzymatic method (A)
0
1
2
3
4
5
6
0 1 2 3 4 5 6
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (E
)
y =R =
1.023x - 0.030.9994
Hitachi , Enzymatic method (A)
Suppl. fig. 12b. Assessment of MacRM commutability by twin plot analysis (Mg)
: Patient sera (n=30) : NIST SRM929a: MacRM-001
0
1
2
3
4
5
6
0 1 2 3 4 5 6
(mg/dL)
Hita
chi ,
Xyl
idyl
blu
e (C
)
y =R =
1.054x - 0.070.9971
Hitachi , Enzymatic method (A)
0
1
2
3
4
5
6
0 1 2 3 4 5 6
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (E
)
y =R =
1.013x - 0.040.9990
Hitachi , Enzymatic method (A)
Suppl. fig. 13a. Assessment of MacRM commutability by twin plot analysis (AST)
: Patient sera (n=29) : JCCLS CRM-001c: MacRM-001
0
100
200
300
400
0 100 200 300 400
(U/L)
Hita
chi ,
JSC
C m
etho
d (B
)
Hitachi , JSCC method (A)
y =R =
0.987x - 1.70.9999
0
100
200
300
400
0 100 200 300 400
(U/L)
Sie
men
s ,
IFC
C m
etho
d
Hitachi , JSCC method (A)
y =R =
1.065x - 5.10.9984
0
100
200
300
400
0 100 200 300 400
(U/L)
Roc
he ,
JSC
C m
etho
d (I
)
Hitachi , JSCC method (A)
y =R =
1.017x - 2.40.9999
0
100
200
300
400
0 100 200 300 400
(U/L)
Fuj
ifilm
,D
ry-c
hem
istr
y (A
)
Hitachi , JSCC method (A)
y =R =
0.906x + 1.20.9995
0
100
200
300
400
0 100 200 300 400
(U/L)
Hita
chi ,
JSC
C m
etho
d (C
)
Hitachi , JSCC method (A)
y =R =
0.966x + 0.10.9999
0
100
200
300
400
0 100 200 300 400
(U/L)
Hita
chi ,
JSC
C m
etho
d (D
)
Hitachi , JSCC method (A)
y =R =
1.031x - 1.60.9999
0
100
200
300
400
0 100 200 300 400
(U/L)
Hita
chi ,
JSC
C m
etho
d (F
)
Hitachi , JSCC method (A)
y =R =
1.025x - 3.30.9999
0
100
200
300
400
0 100 200 300 400
(U/L)
Hita
chi ,
JSC
C m
etho
d (G
)
Hitachi , JSCC method (A)
y =R =
0.987x - 0.60.9999
0
100
200
300
400
0 100 200 300 400
(U/L)
Bec
kman
Cou
lter
,JS
CC
met
hod
(H)
Hitachi , JSCC method (A)
y =R =
0.998x - 0.20.9999
0
100
200
300
400
0 100 200 300 400
(U/L)
Ort
ho ,
Dry
-che
mis
try
(B)
Hitachi , JSCC method (A)
y =R =
1.047x - 3.60.9995
0
100
200
300
400
0 100 200 300 400
(U/L)
Hita
chi ,
JSC
C m
etho
d (E
)
Hitachi , JSCC method (A)
y =R =
1.010x - 2.50.9999
0
100
200
300
400
0 100 200 300 400
(U/L)
Hita
chi ,
JSC
C m
etho
d (J
)
Hitachi , JSCC method (A)
y =R =
0.968x - 0.40.9998
Suppl. fig. 13b. Assessment of MacRM commutability by twin plot analysis (AST)
: Patient sera (n=29) : JCCLS CRM-001c: MacRM-001
0
100
200
300
400
0 100 200 300 400
(U/L)
Hita
chi ,
JSC
C m
etho
d (K
)
Hitachi , JSCC method (A)
y =R =
0.993x - 2.90.9998
0
100
200
300
400
0 100 200 300 400
(U/L)
Hita
chi ,
JSC
C m
etho
d (B
)
Hitachi , JSCC method (A)
y =R =
0.999x - 2.00.9999
Suppl. fig. 14a. Assessment of MacRM commutability by twin plot analysis (ALT)
: Patient sera (n=28) : JCCLS CRM-001c : MacRM-001
0
100
200
300
400
0 100 200 300 400
(U/L)
Hita
chi ,
JSC
C m
etho
d (F
)
Hitachi , JSCC method (A)
y =R =
0.995x - 0.20.9999
0
100
200
300
400
0 100 200 300 400
(U/L)
Hita
chi ,
JSC
C m
etho
d (G
)
Hitachi , JSCC method (A)
y =R =
1.002x - 0.10.9999
0
100
200
300
400
0 100 200 300 400
(U/L)
Hita
chi ,
JSC
C m
etho
d (C
)
Hitachi , JSCC method (A)
y =R =
1.027x + 0.50.9998
0
100
200
300
400
0 100 200 300 400
(U/L)
Hita
chi ,
JSC
C m
etho
d (D
)
Hitachi , JSCC method (A)
y =R =
1.009x + 0.80.9998
0
100
200
300
400
0 100 200 300 400
(U/L)
Roc
he ,
JSC
C m
etho
d (I
)
Hitachi , JSCC method (A)
y =R =
1.009x - 0.40.9998
0
100
200
300
400
0 100 200 300 400
(U/L)
Fuj
ifilm
,D
ry-c
hem
istr
y (A
)
Hitachi , JSCC method (A)
y =R =
0.988x - 2.50.9983
0
100
200
300
400
0 100 200 300 400
(U/L)
Hita
chi ,
JSC
C m
etho
d (B
)
Hitachi , JSCC method (A)
y =R =
0.993x - 0.40.9999
0
100
200
300
400
0 100 200 300 400
(U/L)
Sie
men
s ,
IFC
C m
etho
d
Hitachi , JSCC method (A)
y =R =
1.156x + 2.30.9960
0
100
200
300
400
0 100 200 300 400
(U/L)
Hita
chi ,
JSC
C m
etho
d (E
)
Hitachi , JSCC method (A)
y =R =
1.006x - 0.80.9999
0
100
200
300
400
0 100 200 300 400
(U/L)
Ort
ho ,
Dry
-che
mis
try
(B)
Hitachi , JSCC method (A)
y =R =
1.084x - 7.50.9992
0
100
200
300
400
0 100 200 300 400
(U/L)
Bec
kman
Cou
lter
,JS
CC
met
hod
(H)
Hitachi , JSCC method (A)
y =R =
1.025x - 0.50.9998
0
100
200
300
400
0 100 200 300 400
(U/L)
Hita
chi ,
JSC
C m
etho
d (J
)
Hitachi , JSCC method (A)
y =R =
1.029x + 1.90.9998
Suppl. fig. 14b. Assessment of MacRM commutability by twin plot analysis (ALT)
: Patient sera (n=28) : JCCLS CRM-001c : MacRM-001
0
100
200
300
400
0 100 200 300 400
(U/L)
Hita
chi ,
JSC
C m
etho
d (K
)
Hitachi , JSCC method (A)
y =R =
1.020x + 0.00.9999
0
100
200
300
400
0 100 200 300 400
(U/L)
Hita
chi ,
JSC
C m
etho
d (B
)
Hitachi , JSCC method (A)
y =R =
1.022x - 0.50.9999
Suppl. fig. 15a. Assessment of MacRM commutability by twin plot analysis (ALP)
: Patient sera (n=27) : JCCLS CRM-001c: MacRM-001
0
400
800
1200
0 400 800 1200
(U/L)
Hita
chi ,
JSC
C m
etho
d (B
)
Hitachi , JSCC method (A)
y =R =
1.036x - 4.00.9998
0
400
800
1200
0 400 800 1200
(U/L)
Sie
men
s ,
IFC
C m
etho
d
Hitachi , JSCC method (A)
y =R =
0.383x - 3.60.9989
0
400
800
1200
0 400 800 1200
(U/L)
Roc
he ,
JSC
C m
etho
d (H
)
Hitachi , JSCC method (A)
y =R =
0.985x + 1.80.9998
0
400
800
1200
0 400 800 1200
(U/L)
Fuj
ifilm
,D
ry-c
hem
istr
y (A
)
Hitachi , JSCC method (A)
y =R =
0.999x + 17.80.9933
0
400
800
1200
0 400 800 1200
(U/L)
Hita
chi ,
JSC
C m
etho
d (C
)
Hitachi , JSCC method (A)
y =R =
1.049x - 2.20.9997
0
400
800
1200
0 400 800 1200
(U/L)
Hita
chi ,
JSC
C m
etho
d (D
)
Hitachi , JSCC method (A)
y =R =
1.003x + 0.00.9999
0
400
800
1200
0 400 800 1200
(U/L)
Hita
chi ,
JSC
C m
etho
d (F
)
Hitachi , JSCC method (A)
y =R =
1.017x - 0.70.9999
0
400
800
1200
0 400 800 1200
(U/L)
Hita
chi ,
JSC
C m
etho
d (G
)
Hitachi , JSCC method (A)
y =R =
1.038x - 0.30.9995
0
400
800
1200
0 400 800 1200
(U/L)
Bec
kman
Cou
lter
,JS
CC
met
hod
(J)
Hitachi , JSCC method (A)
y =R =
0.964x + 3.40.9994
0
400
800
1200
0 400 800 1200
(U/L)
Ort
ho ,
Dry
-che
mis
try
(B)
Hitachi , JSCC method (A)
y =R =
0.948x + 33.90.9969
0
400
800
1200
0 400 800 1200
(U/L)
Hita
chi ,
JSC
C m
etho
d (E
)
Hitachi , JSCC method (A)
y =R =
1.001x + 4.00.9997
0
400
800
1200
0 400 800 1200
(U/L)
Hita
chi ,
JSC
C m
etho
d (I
)
Hitachi , JSCC method (A)
y =R =
1.016x - 1.10.9998
Suppl. fig. 15b. Assessment of MacRM commutability by twin plot analysis (ALP)
: Patient sera (n=27) : JCCLS CRM-001c: MacRM-001
0
400
800
1200
0 400 800 1200
(U/L)
Hita
chi ,
JSC
C m
etho
d (K
)
Hitachi , JSCC method (A)
y =R =
1.051x - 3.40.9998
0
400
800
1200
0 400 800 1200
(U/L)
Hita
chi ,
JSC
C m
etho
d (L
)
Hitachi , JSCC method (A)
y =R =
1.049x - 4.90.9998
0
400
800
1200
0 400 800 1200
(U/L)
Hita
chi ,
JSC
C m
etho
d (B
)
Hitachi , JSCC method (A)
y =R =
1.033x - 3.10.9998
0
400
800
1200
0 400 800 1200
(U/L)
Hita
chi ,
JSC
C m
etho
d (M
)
Hitachi , JSCC method (A)
y =R =
1.008x + 3.20.9999
Suppl. fig. 16a. Assessment of MacRM commutability by twin plot analysis (LD)
: Patient sera (n=30) : JCCLS CRM-001c: MacRM-001
0
200
400
600
800
0 200 400 600 800
(U/L)
Hita
chi ,
JSC
C m
etho
d (B
)
Hitachi , JSCC method (A)
y =R =
1.032x - 5.60.9993
0
200
400
600
800
0 200 400 600 800
(U/L)
Sie
men
s ,
IFC
C m
etho
d
Hitachi , JSCC method (A)
y =R =
1.030x - 13.00.9905
0
200
400
600
800
0 200 400 600 800
(U/L)
Roc
he ,
JSC
C m
etho
d (G
)
Hitachi , JSCC method (A)
y =R =
0.978x + 3.70.9998
0
200
400
600
800
0 200 400 600 800
(U/L)
Fuj
ifilm
,D
ry-c
hem
istr
y (A
)
Hitachi , JSCC method (A)
y =R =
1.105x - 5.80.9840
0
200
400
600
800
0 200 400 600 800
(U/L)
Hita
chi ,
JSC
C m
etho
d (C
)
Hitachi , JSCC method (A)
y =R =
0.999x + 2.20.9992
0
200
400
600
800
0 200 400 600 800
(U/L)
Hita
chi ,
JSC
C m
etho
d (D
)
Hitachi , JSCC method (A)
y =R =
1.024x - 3.80.9989
0
200
400
600
800
0 200 400 600 800
(U/L)
Hita
chi ,
JSC
C m
etho
d (F
)
Hitachi , JSCC method (A)
y =R =
1.021x + 0.00.9995
0
200
400
600
800
0 200 400 600 800
(U/L)
Hita
chi ,
JSC
C m
etho
d (H
)
Hitachi , JSCC method (A)
y =R =
1.030x - 6.10.9994
0
200
400
600
800
0 200 400 600 800
(U/L)
Bec
kman
Cou
lter
,JS
CC
met
hod
(J)
Hitachi , JSCC method (A)
y =R =
1.048x - 5.90.9994
0
200
400
600
800
0 200 400 600 800
(U/L)
Ort
ho ,
Dry
-che
mis
try
(B)
Hitachi , JSCC method (A)
y =R =
1.039x + 7.60.9868
0
200
400
600
800
0 200 400 600 800
(U/L)
Hita
chi ,
JSC
C m
etho
d (E
)
Hitachi , JSCC method (A)
y =R =
0.996x + 3.40.9998
0
200
400
600
800
0 200 400 600 800
(U/L)
Hita
chi ,
JSC
C m
etho
d (I
)
Hitachi , JSCC method (A)
y =R =
1.039x - 4.30.9985
Suppl. fig. 16b. Assessment of MacRM commutability by twin plot analysis (LD)
: Patient sera (n=30) : JCCLS CRM-001c: MacRM-001
0
200
400
600
800
0 200 400 600 800
(U/L)
Hita
chi ,
JSC
C m
etho
d (K
)
Hitachi , JSCC method (A)
y =R =
1.056x - 0.60.9964
0
200
400
600
800
0 200 400 600 800
(U/L)
Hita
chi ,
JSC
C m
etho
d (B
)
Hitachi , JSCC method (A)
y =R =
1.036x - 3.10.9989
Suppl. fig. 17a. Assessment of MacRM commutability by twin plot analysis (AMY)
: Patient sera (n=30) : JCCLS CRM-001c: MacRM-001
0
200
400
600
800
0 200 400 600 800
(U/L)
Hita
chi ,
JSC
C m
etho
d-G
5 (A
)
Hitachi , JSCC method-G7 (A)
y =R =
1.061x - 4.30.9932
0
200
400
600
800
0 200 400 600 800
(U/L)
Sie
men
s ,
G3
Hitachi , JSCC method-G7 (A)
y =R =
0.836x - 1.90.9960
0
200
400
600
800
0 200 400 600 800
(U/L)
Roc
he ,
JSC
C m
etho
d-G
7 (B
)
Hitachi , JSCC method-G7 (A)
y =R =
0.978x + 2.00.9999
0
200
400
600
800
0 200 400 600 800
(U/L)
Fuj
ifilm
,D
ry-c
hem
istr
y (A
)
Hitachi , JSCC method-G7 (A)
y =R =
0.939x + 1.00.9957
0
200
400
600
800
0 200 400 600 800
(U/L)
Hita
chi ,
JSC
C m
etho
d-G
2 (A
)
Hitachi , JSCC method-G7 (A)
y =R =
1.029x - 5.90.9912
0
200
400
600
800
0 200 400 600 800
(U/L)
Hita
chi ,
JSC
C m
etho
d-G
4
Hitachi , JSCC method-G7 (A)
y =R =
1.059x - 5.60.9942
0
200
400
600
800
0 200 400 600 800
(U/L)
Hita
chi ,
JSC
C m
etho
d-G
3
Hitachi , JSCC method-G7 (A)
y =R =
0.989x - 1.40.9986
0
200
400
600
800
0 200 400 600 800
(U/L)
Hita
chi ,
JSC
C m
etho
d-G
7 (C
)
Hitachi , JSCC method-G7 (A)
y =R =
0.998x - 0.50.9999
0
200
400
600
800
0 200 400 600 800
(U/L)
Bec
kman
Cou
lter
,JS
CC
met
hod-
G7
(D)
Hitachi , JSCC method-G7 (A)
y =R =
0.968x + 2.40.9998
0
200
400
600
800
0 200 400 600 800
(U/L)
Ort
ho ,
Dry
-che
mis
try
(B)
Hitachi , JSCC method-G7 (A)
y =R =
0.870x + 1.10.9824
0
200
400
600
800
0 200 400 600 800
(U/L)
Hita
chi ,
JSC
C m
etho
d-G
5 (B
)
Hitachi , JSCC method-G7 (A)
y =R =
1.045x - 2.60.9978
0
200
400
600
800
0 200 400 600 800
(U/L)
Hita
chi ,
JSC
C m
etho
d-G
5 (C
)
Median of all method
y =R =
1.016x - 2.90.9973
Suppl. fig. 17b. Assessment of MacRM commutability by twin plot analysis (AMY)
: Patient sera (n=30) : JCCLS CRM-001c: MacRM-001
0
200
400
600
800
0 200 400 600 800
(U/L)
Hita
chi ,
JSC
C m
etho
d-G
2 (B
)
Median of all method
y =R =
1.019x - 4.70.9917
0
200
400
600
800
0 200 400 600 800
(U/L)
Hita
chi ,
JSC
C m
etho
d-G
5 (D
)
Median of all method
y =R =
1.049x - 2.90.9974
0
200
400
600
800
0 200 400 600 800
(U/L)
Hita
chi ,
JSC
C m
etho
d-G
7 (F
)
Median of all method
y =R =
0.998x - 0.10.9999
0
200
400
600
800
0 200 400 600 800
(U/L)
Hita
chi ,
JSC
C m
etho
d-G
7 (C
)
Median of all method
y =R =
0.990x - 0.10.9999
0
200
400
600
800
0 200 400 600 800
(U/L)
Hita
chi ,
JSC
C m
etho
d-G
7 (E
)
Median of all method
y =R =
0.990x - 0.10.9999
Suppl. fig. 18a. Assessment of MacRM commutability by twin plot analysis (CK)
: Patient sera (n=28) : JCCLS CRM-001c: MacRM-001
0
400
800
1200
0 400 800 1200
(U/L)
Hita
chi ,
JSC
C m
etho
d (B
)
Hitachi , JSCC method (A)
y =R =
0.978x + 2.50.9999
0
400
800
1200
0 400 800 1200
(U/L)
Sie
men
s ,
JSC
C m
etho
d (M
)
Hitachi , JSCC method (A)
y =R =
0.982x + 5.40.9999
0
400
800
1200
0 400 800 1200
(U/L)
Roc
he ,
JSC
C m
etho
d (I
)
Hitachi , JSCC method (A)
y =R =
0.970x + 5.10.9998
0
400
800
1200
0 400 800 1200
(U/L)
Fuj
ifilm
,D
ry-c
hem
istr
y (A
)
Hitachi , JSCC method (A)
y =R =
0.924x + 18.30.9974
0
400
800
1200
0 400 800 1200
(U/L)
Hita
chi ,
IFC
C m
etho
d
Hitachi , JSCC method (A)
y =R =
0.999x + 0.70.9999
0
400
800
1200
0 400 800 1200
(U/L)
Hita
chi ,
JSC
C m
etho
d (C
)
Hitachi , JSCC method (A)
y =R =
0.995x + 0.40.9999
0
400
800
1200
0 400 800 1200
(U/L)
Hita
chi ,
JSC
C m
etho
d (E
)
Hitachi , JSCC method (A)
y =R =
0.996x + 0.70.9999
0
400
800
1200
0 400 800 1200
(U/L)
Hita
chi ,
JSC
C m
etho
d (F
)
Hitachi , JSCC method (A)
y =R =
0.990x + 1.40.9999
0
400
800
1200
0 400 800 1200
(U/L)
Bec
kman
Cou
lter
,JS
CC
met
hod
(L)
Hitachi , JSCC method (A)
y =R =
0.934x + 3.70.9988
0
400
800
1200
0 400 800 1200
(U/L)
Ort
ho ,
Dry
-che
mis
try
(B)
Hitachi , JSCC method (A)
y =R =
0.973x + 3.40.9988
0
400
800
1200
0 400 800 1200
(U/L)
Hita
chi ,
JSC
C m
etho
d (D
)
Hitachi , JSCC method (A)
y =R =
1.003x - 0.80.9999
0
400
800
1200
0 400 800 1200
(U/L)
Hita
chi ,
JSC
C m
etho
d (G
)
Hitachi , JSCC method (A)
y =R =
0.983x + 1.50.9999
Suppl. fig. 18b. Assessment of MacRM commutability by twin plot analysis (CK)
: Patient sera (n=28) : JCCLS CRM-001c: MacRM-001
0
400
800
1200
0 400 800 1200
(U/L)
Hita
chi ,
JSC
C m
etho
d (H
)
Hitachi , JSCC method (A)
y =R =
0.980x + 2.20.9999
0
400
800
1200
0 400 800 1200
(U/L)
Hita
chi ,
JSC
C m
etho
d (J
)
Hitachi , JSCC method (A)
y =R =
0.985x + 2.60.9999
0
400
800
1200
0 400 800 1200
(U/L)
Hita
chi ,
JSC
C m
etho
d (C
)
Hitachi , JSCC method (A)
y =R =
1.003x + 1.60.9999
0
400
800
1200
0 400 800 1200
(U/L)
Hita
chi ,
JSC
C m
etho
d (K
)
Hitachi , JSCC method (A)
y =R =
0.973x + 3.20.9999
Suppl. fig. 19a. Assessment of MacRM commutability by twin plot analysis (GGT)
: Patient sera (n=25) : JCCLS CRM-001c: MacRM-001
0
100
200
300
400
500
0 100 200 300 400 500
(U/L)
Hita
chi ,
JSC
C/I
FC
C m
etho
d (B
)
Hitachi , JSCC/IFCC method (A)
y =R =
0.989x - 0.50.9998
0
100
200
300
400
500
0 100 200 300 400 500
(U/L)
Sie
men
s ,
JSC
C/I
FC
C m
etho
d (G
)
Hitachi , JSCC/IFCC method (A)
y =R =
1.131x - 1.10.9998
0
100
200
300
400
500
0 100 200 300 400 500
(U/L)
Roc
he ,
JSC
C/I
FC
C m
etho
d (K
)
Hitachi , JSCC/IFCC method (A)
y =R =
0.968x - 0.70.9999
0
100
200
300
400
500
0 100 200 300 400 500
(U/L)
Fuj
ifilm
,D
ry-c
hem
istr
y (A
)
Hitachi , JSCC/IFCC method (A)
y =R =
0.951x + 2.70.9948
0
100
200
300
400
500
0 100 200 300 400 500
(U/L)
Hita
chi ,
JSC
C/I
FC
C m
etho
d (C
)
Hitachi , JSCC/IFCC method (A)
y =R =
1.003x - 0.40.9999
0
100
200
300
400
500
0 100 200 300 400 500
(U/L)
Hita
chi ,
JSC
C/I
FC
C m
etho
d (D
)
Hitachi , JSCC/IFCC method (A)
y =R =
1.011x + 1.20.9995
0
100
200
300
400
500
0 100 200 300 400 500
(U/L)
Hita
chi ,
JSC
C/I
FC
C m
etho
d (F
)
Hitachi , JSCC/IFCC method (A)
y =R =
0.968x + 0.30.9999
0
100
200
300
400
500
0 100 200 300 400 500
(U/L)
Hita
chi ,
JSC
C/I
FC
C m
etho
d (H
)
Hitachi , JSCC/IFCC method (A)
y =R =
0.990x + 0.20.9999
0
100
200
300
400
500
0 100 200 300 400 500
(U/L)
Bec
kman
Cou
lter
,JS
CC
/IF
CC
met
hod
(J)
Hitachi , JSCC/IFCC method (A)
y =R =
0.959x + 1.50.9997
0
100
200
300
400
500
0 100 200 300 400 500
(U/L)
Ort
ho ,
Dry
-che
mis
try
(B)
Hitachi , JSCC/IFCC method (A)
y =R =
1.059x - 1.90.9977
0
100
200
300
400
500
0 100 200 300 400 500
(U/L)
Hita
chi ,
JSC
C/I
FC
C m
etho
d (E
)
Hitachi , JSCC/IFCC method (A)
y =R =
1.019x - 0.40.9998
0
100
200
300
400
500
0 100 200 300 400 500
(U/L)
Hita
chi ,
JSC
C/I
FC
C m
etho
d (I
)
Hitachi , JSCC/IFCC method (A)
y =R =
0.984x - 0.80.9999
Suppl. fig. 19b. Assessment of MacRM commutability by twin plot analysis (GGT)
: Patient sera (n=25) : JCCLS CRM-001c: MacRM-001
0
100
200
300
400
500
0 100 200 300 400 500
(U/L)
Hita
chi ,
JSC
C/I
FC
C m
etho
d (L
)
Hitachi , JSCC/IFCC method (A)
y =R =
0.969x + 0.60.9998
0
100
200
300
400
500
0 100 200 300 400 500
(U/L)
Hita
chi ,
JSC
C/I
FC
C m
etho
d (C
)
Hitachi , JSCC/IFCC method (A)
y =R =
0.991x + 0.40.9999
Suppl. fig. 20. Assessment of MacRM commutability by twin plot analysis (ChE)
: Patient sera (n=30) : JCCLS CRM-002c: MacRM-001
0
100
200
300
400
500
0 100 200 300 400 500
(U/L)
Hita
chi ,
JSC
C (
p-H
ydro
xybe
nzoy
lcho
line)
(B
)
Hitachi , JSCC method (p-HBC) (A)
y =R =
1.006x + 1.20.9997
0
100
200
300
400
500
0 100 200 300 400 500
(U/L)
Hita
chi ,
JSC
C (
5-M
ethy
l-2-
then
oylth
ioch
olin
e)
Hitachi , JSCC method (p-HBC) (A)
y =R =
1.021x + 2.10.9988
0
100
200
300
400
500
0 100 200 300 400 500
(U/L)
Hita
chi ,
JSC
C (
2,3-
Dim
etho
xybe
nzoy
lthio
chol
ine)
Hitachi , JSCC method (p-HBC) (A)
y =R =
1.007x + 8.50.9993
0
100
200
300
400
500
0 100 200 300 400 500
(U/L)
Fuj
ifilm
,D
ry-c
hem
istr
y (A
)
Hitachi , JSCC method (p-HBC) (A)
y =R =
0.956x + 7.30.9991
0
100
200
300
400
500
0 100 200 300 400 500
(U/L)
Hita
chi ,
JSC
C (
p-H
ydro
xybe
nzoy
lcho
line)
(C
)
Hitachi , JSCC method (p-HBC) (A)
y =R =
1.000x + 1.70.9999
0
100
200
300
400
500
0 100 200 300 400 500
(U/L)
Hita
chi ,
JSC
C (
Ben
zoyl
thio
chol
ine)
(B
)
Hitachi , JSCC method (p-HBC) (A)
y =R =
0.993x + 6.30.9986
0
100
200
300
400
500
0 100 200 300 400 500
(U/L)
Hita
chi ,
JSC
C (
p-H
ydro
xybe
nzoy
lcho
line)
(A
)
Hitachi , JSCC method (p-HBC) (A)
y =R =
1.010x - 1.50.9998
0
100
200
300
400
500
0 100 200 300 400 500
(U/L)
Hita
chi ,
JSC
C (
p-H
ydro
xybe
nzoy
lcho
line)
(A
)
Hitachi , JSCC method (p-HBC) (A)
y =R =
1.010x - 1.40.9999
0
100
200
300
400
500
0 100 200 300 400 500
(U/L)
Hita
chi ,
JSC
C (
Ben
zoyl
thio
chol
ine)
(A
)
Hitachi , JSCC method (p-HBC) (A)
y =R =
1.003x + 6.00.9991
0
100
200
300
400
500
0 100 200 300 400 500
(U/L)
Ort
ho ,
Dry
-che
mis
try
(B)
Hitachi , JSCC method (p-HBC) (A)
y =R =
0.993x + 5.20.9964
0
100
200
300
400
500
0 100 200 300 400 500
(U/L)
Hita
chi ,
JSC
C (
p-H
ydro
xybe
nzoy
lcho
line)
(D
)
Hitachi , JSCC method (p-HBC) (A)
y =R =
1.023x + 0.00.9998
Suppl. fig. 21. Assessment of MacRM commutability by twin plot analysis (TC)
: Patient sera (n=30) : NIST SRM1951c: MacRM-001
0
100
200
300
0 100 200 300
(mg/dL)
Hita
chi ,
Cho
lest
erol
oxi
dase
-per
oxid
ase
(B)
Hitachi , Cholesterol oxidase-POD (A)
y =R =
1.000x - 3.70.9996
0
100
200
300
0 100 200 300
(mg/dL)
Sie
men
s ,
Cho
lest
erol
oxi
dase
-per
oxid
ase
(I)
Hitachi , Cholesterol oxidase-POD (A)
y =R =
0.940x - 1.90.9899
0
100
200
300
0 100 200 300
(mg/dL)
JEO
L ,
Cho
lest
erol
deh
ydro
gena
se
Hitachi , Cholesterol oxidase-POD (A)
y =R =
0.981 + 0.90.9997
0
100
200
300
0 100 200 300
(mg/dL)
Fuj
ifilm
,D
ry-c
hem
istr
y (A
)
Hitachi , Cholesterol oxidase-POD (A)
y =R =
1.010x - 1.80.9931
0
100
200
300
0 100 200 300
(mg/dL)
Tos
hiba
,C
hole
ster
ol o
xida
se-p
erox
idas
e (F
)
Hitachi , Cholesterol oxidase-POD (A)
y =R =
1.007x + 0.00.9998
0
100
200
300
0 100 200 300
(mg/dL)
Hita
chi ,
Cho
lest
erol
oxi
dase
-per
oxid
ase
(C)
Hitachi , Cholesterol oxidase-POD (A)
y =R =
0.984x + 1.40.9996
0
100
200
300
0 100 200 300
(mg/dL)
Hita
chi ,
Cho
lest
erol
oxi
dase
-per
oxid
ase
(E)
Hitachi , Cholesterol oxidase-POD (A)
y =R =
1.000x + 0.90.9995
0
100
200
300
0 100 200 300
(mg/dL)
Hita
chi ,
Cho
lest
erol
oxi
dase
-per
oxid
ase
(G)
Hitachi , Cholesterol oxidase-POD (A)
y =R =
1.025x - 4.70.9995
0
100
200
300
0 100 200 300
(mg/dL)
Bec
kman
Cou
lter
,C
hole
ster
ol o
xida
se-p
erox
idas
e (H
)
Hitachi , Cholesterol oxidase-POD (A)
y =R =
1.001x - 3.70.9951
0
100
200
300
0 100 200 300
(mg/dL)
Ort
ho ,
Dry
-che
mis
try
(B)
Hitachi , Cholesterol oxidase-POD (A)
y =R =
0.924x + 5.30.9960
0
100
200
300
0 100 200 300
(mg/dL)
Hita
chi ,
Cho
lest
erol
oxi
dase
-per
oxid
ase
(D)
Hitachi , Cholesterol oxidase-POD (A)
y =R =
0.995x + 0.10.9996
0
100
200
300
0 100 200 300
(mg/dL)
Hita
chi ,
Cho
lest
erol
oxi
dase
-per
oxid
ase
(J)
Hitachi , Cholesterol oxidase-POD (A)
y =R =
1.012x - 0.40.9996
Suppl. fig. 22a. Assessment of MacRM commutability by twin plot analysis (TG)
: Patient sera (n=25) : NIST SRM1951c: MacRM-001
0
50
100
150
200
0 50 100 150 200
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (B
)
Hitachi , Enzymatic method (A)
y =R =
1.009x - 2.10.9997
0
50
100
150
200
0 50 100 150 200
(mg/dL)
Sie
men
s ,
Enz
ymat
ic m
etho
d (L
)
Hitachi , Enzymatic method (A)
y =R =
0.972x - 0.70.9964
0
50
100
150
200
0 50 100 150 200
(mg/dL)
JEO
L ,
Enz
ymat
ic m
etho
d (I
)
Hitachi , Enzymatic method (A)
y =R =
0.953x + 5.20.9981
0
50
100
150
200
0 50 100 150 200
(mg/dL)
Fuj
ifilm
,D
ry-c
hem
istr
y (A
)
Hitachi , Enzymatic method (A)
y =R =
1.035x - 1.20.9958
0
50
100
150
200
0 50 100 150 200
(mg/dL)
Tos
hiba
,E
nzym
atic
met
hod
(F)
Hitachi , Enzymatic method (A)
y =R =
1.032x + 0.80.9995
0
50
100
150
200
0 50 100 150 200
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (C
)
Hitachi , Enzymatic method (A)
y =R =
0.998x - 1.30.9995
0
50
100
150
200
0 50 100 150 200
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (E
)
Hitachi , Enzymatic method (A)
y =R =
0.970x + 2.10.9991
0
50
100
150
200
0 50 100 150 200
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (G
)
Hitachi , Enzymatic method (A)
y =R =
0.984x - 0.20.9994
0
50
100
150
200
0 50 100 150 200
(mg/dL)
Bec
kman
Cou
lter
,E
nzym
atic
met
hod
(J)
Hitachi , Enzymatic method (A)
y =R =
0.901x + 2.40.9982
0
50
100
150
200
0 50 100 150 200
(mg/dL)
Ort
ho ,
Dry
-che
mis
try
(B)
Hitachi , Enzymatic method (A)
y =R =
0.977x + 5.40.9917
0
50
100
150
200
0 50 100 150 200
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (D
)
Hitachi , Enzymatic method (A)
y =R =
0.978x + 1.30.9995
0
50
100
150
200
0 50 100 150 200
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (H
)
Hitachi , Enzymatic method (A)
y =R =
1.010x - 1.80.9995
Suppl. fig. 22b. Assessment of MacRM commutability by twin plot analysis (TG)
: Patient sera (n=25) : NIST SRM1951c: MacRM-001
0
50
100
150
200
0 50 100 150 200
(mg/dL)
Hita
chi ,
Enz
ymat
ic m
etho
d (K
)
Hitachi , Enzymatic method (A)
y =R =
1.019x + 0.30.9994
Suppl. fig. 23. Assessment of MacRM commutability by twin plot analysis (HDL-C)
: Patient sera (n=29) : NIST SRM1951c : MacRM-001
0
20
40
60
80
0 20 40 60 80
(mg/dL)
Hita
chi ,
Hom
ogen
eous
ass
ay (
G)
Hitachi , Homogeneous assay (A)
y =R =
0.990x + 0.90.9807
0
20
40
60
80
0 20 40 60 80
(mg/dL)
Hita
chi ,
Hom
ogen
eous
ass
ay (
B)
Hitachi , Homogeneous assay (A)
y =R =
0.979x + 1.40.9785
0
20
40
60
80
0 20 40 60 80
(mg/dL)
Tos
hiba
,H
omog
eneo
us a
ssay
(C
)
Hitachi , Homogeneous assay (A)
y =R =
1.075x - 0.30.9831
0
20
40
60
80
0 20 40 60 80
(mg/dL)
Hita
chi ,
Hom
ogen
eous
ass
ay (
D)
Hitachi , Homogeneous assay (A)
y =R =
0.810x + 10.30.9775
0
20
40
60
80
0 20 40 60 80
(mg/dL)
JEO
L ,
Hom
ogen
eous
ass
ay (
A)
Hitachi , Homogeneous assay (A)
y =R =
1.023x - 0.60.9997
0
20
40
60
80
0 20 40 60 80
(mg/dL)
Fuj
ifilm
,D
ry-c
hem
istr
y (A
)
Hitachi , Homogeneous assay (A)
y =R =
1.021x - 2.30.9850
0
20
40
60
80
0 20 40 60 80
(mg/dL)
Hita
chi ,
Hom
ogen
eous
ass
ay (
B)
Hitachi , Homogeneous assay (A)
y =R =
0.993x + 1.40.9821
0
20
40
60
80
0 20 40 60 80
(mg/dL)
Sie
men
s ,
Hom
ogen
eous
ass
ay (
E)
Hitachi , Homogeneous assay (A)
y =R =
1.024x + 0.60.9944
0
20
40
60
80
0 20 40 60 80
(mg/dL)
Hita
chi ,
Hom
ogen
eous
ass
ay (
F)
Hitachi , Homogeneous assay (A)
y =R =
0.875x + 6.90.9938
0
20
40
60
80
0 20 40 60 80
(mg/dL)
Ort
ho ,
Dry
-che
mis
try
(B)
Hitachi , Homogeneous assay (A)
y =R =
0.894x + 2.70.9864
0
20
40
60
80
0 20 40 60 80
(mg/dL)
Bec
kman
Cou
lter
,H
omog
eneo
us a
ssay
(H
)
Hitachi , Homogeneous assay (A)
y =R =
1.003x + 1.40.9931
Suppl. fig. 24. Assessment of MacRM commutability by twin plot analysis (LDL-C)
: Patient sera (n=29) : NIST SRM1951c : MacRM-001
0
50
100
150
0 50 100 150
(mg/dL)
Hita
chi ,
Hom
ogen
eous
ass
ay (
A)
Hitachi , Homogeneous assay (A)
y =R =
0.998x + 0.60.9993
0
50
100
150
0 50 100 150
(mg/dL)
Hita
chi ,
Hom
ogen
eous
ass
ay (
C)
Hitachi , Homogeneous assay (A)
y =R =
1.024x - 8.00.9868
0
50
100
150
0 50 100 150
(mg/dL)
Hita
chi ,
Hom
ogen
eous
ass
ay (
D)
Hitachi , Homogeneous assay (A)
y =R =
0.952x + 2.60.9949
0
50
100
150
0 50 100 150
(mg/dL)
JEO
L ,
Hom
ogen
eous
ass
ay (
C)
Hitachi , Homogeneous assay (A)
y =R =
1.044x - 7.40.9901
0
50
100
150
0 50 100 150
(mg/dL)
Tos
hiba
,H
omog
eneo
us a
ssay
(E
)
Hitachi , Homogeneous assay (A)
y =R =
1.042x + 2.00.9921
0
50
100
150
0 50 100 150
(mg/dL)
Hita
chi ,
Hom
ogen
eous
ass
ay (
B)
Hitachi , Homogeneous assay (A)
y =R =
1.024x - 9.10.9854
0
50
100
150
0 50 100 150
(mg/dL)
Sie
men
s ,
Hom
ogen
eous
ass
ay (
F)
Hitachi , Homogeneous assay (A)
y =R =
0.961x + 2.80.9989
0
50
100
150
0 50 100 150
(mg/dL)
Hita
chi ,
Hom
ogen
eous
ass
ay (
G)
Hitachi , Homogeneous assay (A)
y =R =
0.981x + 1.80.9998
0
50
100
150
0 50 100 150
(mg/dL)
Ort
ho ,
Dry
-che
mis
try
Hitachi , Homogeneous assay (A)
y =R =
0.927x + 8.20.9749
0
50
100
150
0 50 100 150
(mg/dL)
Bec
kman
Cou
lter
,H
omog
eneo
us a
ssay
(H
)
Hitachi , Homogeneous assay (A)
y =R =
1.016x - 2.00.9987
Suppl. fig. 25a. Assessment of MacRM commutability by twin plot analysis (CRP)
: Patient sera (n=28) : IRMM ERM-DA474: MacRM-001
0
2
4
6
8
10
12
0 2 4 6 8 10 12
(mg/dL)
Hita
chi ,
Lat
ex-t
urbi
dim
etry
(B
)
Hitachi , Latex-turbidimetry (A)
y =R =
0.944x + 0.040.9998
0
2
4
6
8
10
12
0 2 4 6 8 10 12
(mg/dL)
Sie
men
s ,
Lat
ex-t
urbi
dim
etry
(J)
Hitachi , Latex-turbidimetry (A)
y =R =
0.868x + 0.100.9990
0
2
4
6
8
10
12
0 2 4 6 8 10 12
(mg/dL)
JEO
L ,
Lat
ex-t
urbi
dim
etry
(G
)
Hitachi , Latex-turbidimetry (A)
y =R =
0.860x + 0.060.9991
0
2
4
6
8
10
12
0 2 4 6 8 10 12
(mg/dL)
Fuj
ifilm
,D
ry-c
hem
istr
y (A
)
Hitachi , Latex-turbidimetry (A)
y =R =
0.991x - 0.230.9922
0
2
4
6
8
10
12
0 2 4 6 8 10 12
(mg/dL)
Hita
chi ,
Lat
ex-t
urbi
dim
etry
(C
)
Hitachi , Latex-turbidimetry (A)
y =R =
0.940x + 0.020.9994
0
2
4
6
8
10
12
0 2 4 6 8 10 12
(mg/dL)
Hita
chi ,
Lat
ex-t
urbi
dim
etry
(D
)
Hitachi , Latex-turbidimetry (A)
y =R =
0.937x + 0.080.9996
0
2
4
6
8
10
12
0 2 4 6 8 10 12
(mg/dL)
Hita
chi ,
Lat
ex-t
urbi
dim
etry
(F
)
Hitachi , Latex-turbidimetry (A)
y =R =
0.930x + 0.060.9997
0
2
4
6
8
10
12
0 2 4 6 8 10 12
(mg/dL)
Hita
chi ,
Lat
ex-t
urbi
dim
etry
(G
)
Hitachi , Latex-turbidimetry (A)
y =R =
0.860x + 0.070.9991
0
2
4
6
8
10
12
0 2 4 6 8 10 12
(mg/dL)
Bec
kman
Cou
lter
,L
atex
-tur
bidi
met
ry (
M)
Hitachi , Latex-turbidimetry (A)
y =R =
1.007x - 0.100.9971
0
2
4
6
8
10
12
0 2 4 6 8 10 12
(mg/dL)
Ort
ho ,
Dry
-che
mis
try
(B)
Hitachi , Latex-turbidimetry (A)
y =R =
1.107x + 0.490.9841
0
2
4
6
8
10
12
0 2 4 6 8 10 12
(mg/dL)
Hita
chi ,
Lat
ex-t
urbi
dim
etry
(E
)
Hitachi , Latex-turbidimetry (A)
y =R =
0.924x + 0.020.9998
0
2
4
6
8
10
12
0 2 4 6 8 10 12
(mg/dL)
Hita
chi ,
Lat
ex-t
urbi
dim
etry
(H
)
Hitachi , Latex-turbidimetry (A)
y =R =
0.958x + 0.010.9999
Suppl. fig. 25b. Assessment of MacRM commutability by twin plot analysis (CRP)
: Patient sera (n=28) : IRMM ERM-DA474: MacRM-001
0
2
4
6
8
10
12
0 2 4 6 8 10 12
(mg/dL)
Hita
chi ,
Lat
ex-t
urbi
dim
etry
(I)
Hitachi , Latex-turbidimetry (A)
y =R =
0.935x + 0.080.9994
0
2
4
6
8
10
12
0 2 4 6 8 10 12
(mg/dL)
Hita
chi ,
Lat
ex-t
urbi
dim
etry
(K
)
Hitachi , Latex-turbidimetry (A)
y =R =
0.908x + 0.150.9973
0
2
4
6
8
10
12
0 2 4 6 8 10 12
(mg/dL)
Hita
chi ,
Lat
ex-t
urbi
dim
etry
(N
)
Hitachi , Latex-turbidimetry (A)
y =R =
0.971x + 0.040.9998
0
2
4
6
8
10
12
0 2 4 6 8 10 12
(mg/dL)
Hita
chi ,
Lat
ex-t
urbi
dim
etry
(D
)
Hitachi , Latex-turbidimetry (A)
y =R =
0.888x + 0.060.9995
0
2
4
6
8
10
12
0 2 4 6 8 10 12
(mg/dL)
Hita
chi ,
Lat
ex-t
urbi
dim
etry
(L
)
Hitachi , Latex-turbidimetry (A)
y =R =
1.000x + 0.050.9999
Suppl. fig. 26a. Assessment of MacRM commutability by twin plot analysis (Alb)
: Patient sera (n=30) : IRMM ERM-DA470k: MacRM-001
1
2
3
4
5
1 2 3 4 5
(g/dL)
Hita
chi ,
BC
P (
B)
y =R =
0.949x + 0.130.9997
Hitachi , BCP (A)
1
2
3
4
5
1 2 3 4 5
(g/dL)
Sie
men
s ,
BC
P (
E)
y =R =
0.908x + 0.170.9973
Hitachi , BCP (A)
1
2
3
4
5
1 2 3 4 5
(g/dL)
Hita
chi ,
BC
G (
A)
y =R =
0.912x + 0.530.9978
Hitachi , BCP (A)
1
2
3
4
5
1 2 3 4 5
(g/dL)
Fuj
ifilm
,D
ry-c
hem
istr
y (A
)
y =R =
0.860x + 0.600.9902
Hitachi , BCP (A)
1
2
3
4
5
1 2 3 4 5
(g/dL)
Hita
chi ,
BC
G (
B)
y =R =
0.886x + 0.530.9973
Hitachi , BCP (A)
1
2
3
4
5
1 2 3 4 5
(g/dL)
Hita
chi ,
BC
P (
C)
y =R =
1.008x - 0.010.9996
Hitachi , BCP (A)
1
2
3
4
5
1 2 3 4 5
(g/dL)
Hita
chi ,
BC
G (
C)
y =R =
0.787x + 1.030.9940
Hitachi , BCP (A)
1
2
3
4
5
1 2 3 4 5
(g/dL)
Hita
chi ,
BC
P (
F)
y =R =
0.976x + 0.070.9989
Hitachi , BCP (A)
1
2
3
4
5
1 2 3 4 5
(g/dL)
Tos
hiba
,B
CG
(F
)
y =R =
0.724x + 1.120.9942
Hitachi , BCP (A)
1
2
3
4
5
1 2 3 4 5
(g/dL)
Ort
ho ,
Dry
-che
mis
try
(B)
y =R =
0.895x + 0.360.9915
Hitachi , BCP (A)
1
2
3
4
5
1 2 3 4 5
(g/dL)
Hita
chi ,
BC
P (
D)
y =R =
0.960x + 0.030.9995
Hitachi , BCP (A)
1
2
3
4
5
1 2 3 4 5
(g/dL)
Hita
chi ,
BC
P (
G)
y =R =
1.011x + 0.010.9997
Hitachi , BCP (A)
Suppl. fig. 26b. Assessment of MacRM commutability by twin plot analysis (Alb)
: Patient sera (n=30) : IRMM ERM-DA470k: MacRM-001
1
2
3
4
5
1 2 3 4 5
(g/dL)
Hita
chi ,
BC
G (
D)
y =R =
0.948x + 0.440.9952
Hitachi , BCP (A)
1
2
3
4
5
1 2 3 4 5
(g/dL)
Hita
chi ,
BC
P (
H)
y =R =
1.014x + 0.040.9996
Hitachi , BCP (A)
1
2
3
4
5
1 2 3 4 5
(g/dL)
Hita
chi ,
BC
G (
E)
y =R =
0.907x + 0.550.9972
Hitachi , BCP (A)
1
2
3
4
5
1 2 3 4 5
(g/dL)
Hita
chi ,
BC
P (
J)
y =R =
1.002x + 0.040.9993
Hitachi , BCP (A)
1
2
3
4
5
1 2 3 4 5
(g/dL)
Hita
chi ,
BC
P (
A)
y =R =
0.995x - 0.010.9997
Hitachi , BCP (A)
1
2
3
4
5
1 2 3 4 5
(g/dL)
Hita
chi ,
BC
P (
B)
y =R =
0.965x + 0.080.9997
Hitachi , BCP (A)
1
2
3
4
5
1 2 3 4 5
(g/dL)
Hita
chi ,
BC
P (
I)
y =R =
0.998x + 0.030.9996
Hitachi , BCP (A)
1
2
3
4
5
1 2 3 4 5
(g/dL)
Hita
chi ,
BC
G (
G)
y =R =
0.879x + 0.520.9984
Hitachi , BCP (A)
Suppl. fig. 27. Assessment of MacRM commutability by twin plot analysis (IgG)
: Patient sera (n=30) : IRMM ERM-DA470k: MacRM-001
500
1000
1500
2000
500 1000 1500 2000
(mg/dL)
Hita
chi ,
Tur
bidi
met
ry (
B)
Hitachi , Turbidimetry (A)
y =R =
1.078x - 570.9982
500
1000
1500
2000
500 1000 1500 2000
(mg/dL)
Sie
men
s ,
Nep
helo
met
ry
Hitachi , Turbidimetry (A)
y =R =
0.949x + 1230.9956
500
1000
1500
2000
500 1000 1500 2000
(mg/dL)
Hita
chi ,
Tur
bidi
met
ry (
D)
Hitachi , Turbidimetry (A)
y =R =
0.875x + 1310.9979
500
1000
1500
2000
500 1000 1500 2000
(mg/dL)
Ort
ho ,
Dry
-che
mis
try
Hitachi , Turbidimetry (A)
y =R =
0.914x + 1350.9954
500
1000
1500
2000
500 1000 1500 2000
(mg/dL)
Hita
chi ,
Tur
bidi
met
ry (
D)
Hitachi , Turbidimetry (A)
y =R =
0.981x + 70.9982
500
1000
1500
2000
500 1000 1500 2000
(mg/dL)
Hita
chi ,
Tur
bidi
met
ry (
C)
Hitachi , Turbidimetry (A)
y =R =
0.951x + 850.9975
500
1000
1500
2000
500 1000 1500 2000
(mg/dL)
Hita
chi ,
Tur
bidi
met
ry (
A)
Hitachi , Turbidimetry (A)
y =R =
1.029x - 190.9980
Suppl. fig. 28. Assessment of MacRM commutability by twin plot analysis (IgA)
: Patient sera (n=28) : IRMM ERM-DA470k: MacRM-001
0
100
200
300
400
500
0 100 200 300 400 500
(mg/dL)
Hita
chi ,
Tur
bidi
met
ry (
B)
Hitachi , Turbidimetry (A)
y =R =
0.983x - 3.30.9994
0
100
200
300
400
500
0 100 200 300 400 500
(mg/dL)
Sie
men
s ,
Nep
helo
met
ry
Hitachi , Turbidimetry (A)
y =R =
1.009x + 21.00.9963
0
100
200
300
400
500
0 100 200 300 400 500
(mg/dL)
Hita
chi ,
Tur
bidi
met
ry (
D)
Hitachi , Turbidimetry (A)
y =R =
0.847x + 19.40.9964
0
100
200
300
400
500
0 100 200 300 400 500
(mg/dL)
Ort
ho ,
Dry
-che
mis
try
Hitachi , Turbidimetry (A)
y =R =
1.115x - 19.80.9989
0
100
200
300
400
500
0 100 200 300 400 500
(mg/dL)
Hita
chi ,
Tur
bidi
met
ry (
D)
Hitachi , Turbidimetry (A)
y =R =
0.966x + 4.80.9996
0
100
200
300
400
500
0 100 200 300 400 500
(mg/dL)
Hita
chi ,
Tur
bidi
met
ry (
C)
Hitachi , Turbidimetry (A)
y =R =
1.023x - 6.80.9996
0
100
200
300
400
500
0 100 200 300 400 500
(mg/dL)
Hita
chi ,
Tur
bidi
met
ry (
A)
Hitachi , Turbidimetry (A)
y =R =
0.988x + 0.40.9996
Suppl. fig. 29. Assessment of MacRM commutability by twin plot analysis (IgM)
: Patient sera (n=28) : IRMM ERM-DA470k: MacRM-001
0
50
100
150
200
0 50 100 150 200
(mg/dL)
Hita
chi ,
Tur
bidi
met
ry (
B)
Hitachi , Turbidimetry (A)
y =R =
0.990x - 1.00.9998
0
50
100
150
200
0 50 100 150 200
(mg/dL)
Sie
men
s ,
Nep
helo
met
ry
Hitachi , Turbidimetry (A)
y =R =
0.977x + 6.50.9856
0
50
100
150
200
0 50 100 150 200
(mg/dL)
Hita
chi ,
Tur
bidi
met
ry (
D)
Hitachi , Turbidimetry (A)
y =R =
1.015x - 2.10.9997
0
50
100
150
200
0 50 100 150 200
(mg/dL)
Ort
ho ,
Dry
-che
mis
try
Hitachi , Turbidimetry (A)
y =R =
0.997x + 1.70.9977
0
50
100
150
200
0 50 100 150 200
(mg/dL)
Hita
chi ,
Tur
bidi
met
ry (
D)
Hitachi , Turbidimetry (A)
y =R =
1.004x - 3.20.9998
0
50
100
150
200
0 50 100 150 200
(mg/dL)
Hita
chi ,
Tur
bidi
met
ry (
C)
Hitachi , Turbidimetry (A)
y =R =
0.960x - 2.60.9992
0
50
100
150
200
0 50 100 150 200
(mg/dL)
Hita
chi ,
Tur
bidi
met
ry (
A)
Hitachi , Turbidimetry (A)
y =R =
0.977x + 5.80.9994
Suppl. fig. 30a. Assessment of MacRM commutability by twin plot analysis (TP)
: Patient sera (n=28): MacRM-001
4
5
6
7
8
4 5 6 7 8
(g/dL)
Hita
chi ,
Biu
ret (
B)
y =R =
0.991x + 0.120.9995
Hitachi , Biuret (A)
4
5
6
7
8
4 5 6 7 8
(g/dL)
Sie
men
s ,
Biu
ret (
H)
y =R =
1.003x + 0.170.9974
Hitachi , Biuret (A)
4
5
6
7
8
4 5 6 7 8
(g/dL)
Hita
chi ,
Biu
ret (
C)
y =R =
1.008x - 0.090.9996
Hitachi , Biuret (A)
4
5
6
7
8
4 5 6 7 8
(g/dL)
Fuj
ifilm
,D
ry-c
hem
istr
y (A
)
y =R =
0.993x + 0.060.9974
Hitachi , Biuret (A)
4
5
6
7
8
4 5 6 7 8
(g/dL)
Hita
chi ,
Biu
ret (
D)
y =R =
1.007x - 0.030.9994
Hitachi , Biuret (A)
4
5
6
7
8
4 5 6 7 8
(g/dL)
Hita
chi ,
Biu
ret (
E)
y =R =
1.024x + 0.180.9957
Hitachi , Biuret (A)
4
5
6
7
8
4 5 6 7 8
(g/dL)
Hita
chi ,
Biu
ret (
G)
y =R =
1.002x - 0.010.9979
Hitachi , Biuret (A)
4
5
6
7
8
4 5 6 7 8
(g/dL)
Hita
chi ,
Biu
ret (
I)
y =R =
0.961x + 0.460.9977
Hitachi , Biuret (A)
4
5
6
7
8
4 5 6 7 8
(g/dL)
Tos
hiba
,B
iure
t (N
)
y =R =
1.033x + 0.100.9945
Hitachi , Biuret (A)
4
5
6
7
8
4 5 6 7 8
(g/dL)
Ort
ho ,
Dry
-che
mis
try
(B)
y =R =
1.114x - 0.700.9981
Hitachi , Biuret (A)
4
5
6
7
8
4 5 6 7 8
(g/dL)
Hita
chi ,
Biu
ret (
F)
y =R =
0.990x + 0.090.9982
Hitachi , Biuret (A)
4
5
6
7
8
4 5 6 7 8
(g/dL)
Hita
chi ,
Biu
ret (
J)
y =R =
1.022x - 0.030.9996
Hitachi , Biuret (A)
Suppl. fig. 30b. Assessment of MacRM commutability by twin plot analysis (TP)
: Patient sera (n=28): MacRM-001
4
5
6
7
8
4 5 6 7 8
(g/dL)
Hita
chi ,
Biu
ret (
K)
y =R =
1.020x + 0.040.9971
Hitachi , Biuret (A)
4
5
6
7
8
4 5 6 7 8
(g/dL)
Hita
chi ,
Biu
ret (
L)
y =R =
0.973x + 0.220.9978
Hitachi , Biuret (A)
4
5
6
7
8
4 5 6 7 8
(g/dL)
Hita
chi ,
Biu
ret (
O)
y =R =
0.970x + 0.230.9985
Hitachi , Biuret (A)
4
5
6
7
8
4 5 6 7 8
(g/dL)
Hita
chi ,
Biu
ret (
C)
y =R =
1.000x - 0.100.9994
Hitachi , Biuret (A)
4
5
6
7
8
4 5 6 7 8
(g/dL)
Hita
chi ,
Biu
ret (
M)
y =R =
0.978x + 0.210.9990
Hitachi , Biuret (A)
4
5
6
7
8
4 5 6 7 8
(g/dL)
Hita
chi ,
Biu
ret (
K)
y =R =
1.002x + 0.110.9985
Hitachi , Biuret (A)