supplemental figure 2
DESCRIPTION
Supplemental Figure 2 - PowerPoint PPT PresentationTRANSCRIPT
Supplemental Figure 2
RPMI8226 cells were incubated with increasing concentrations of the respective HIV PI (0-160 M; Am: amprenavir; At: atazanavir; D: darunavir; I: indinavir; T: tipranavir) and intracellular proteasome activity was assessed using the affinity probe MV151. The bargraphs represent relative changes in proteasome subunit-specific (1/5 vs. 2) fluorescence signals compared to untreated controls.
RPMI8226 MV-151
β- actin
[µM]
β1(i)/β5(i)
β2β2i
[kD]30
20
45
β2/2iβ5/5i/1/1i
Cha
nge
in p
rote
asom
e ac
tivity
[%
]
NLVS 0 10 20 40 0 10 20 40 10 20 40 NLVS 0 10 20 40 0 10 20 40
Am At D I T