supplementary figure 1. ca19-9 and sle x expression correlated with fut3 and fut6
DESCRIPTION
B. A. HT29. HCT15. Colo205. Ls174T. LS180. Isotypic control. concentration (IU/ml/10 6 cells). 7.6%. 0.8%. 95.3%. 26.3%. 42.2%. CA19-9. Cell number. FITC. LS180. FUT3. FUT6. HT29. HCT15. Colo205. Ls174T. C. Isotypic control. Relative expression ( / ß-actin). 50.2%. - PowerPoint PPT PresentationTRANSCRIPT
Supplementary Figure 1. CA19-9 and sLex expression correlated with FUT3 and FUT6
A
C
95.3%7.6% 0.8% 26.3%
HT29 HCT15 Colo205 Ls174T
FITC
Cel
l nu
mb
er
Isotypic control
CA19-9
B
con
cen
trat
ion
(IU
/ml/1
06 c
ells
)R
elat
ive
exp
ress
ion
( /
ß-a
ctin
) FUT3 FUT6
Cel
l nu
mb
er
FITC
sLex99.83%50.2% 0.7%
LS180
42.2%
Isotypic control
57.4% 88.7%
HT29 HCT15 Colo205 Ls174T LS180
A B
FUT3
LS174TColo205 No
treat
Rando
m
ß-actin
FUT6
siFUT3
siFUT3
+ siF
UT6
No tre
at
Rando
m
siFUT6
siFUT3
+ siF
UT6
ß-actin
Supplementary Figure 2. Suppression of FUT3 and/or FUT6 by siRNAs
1.0 0.94 0.23 0.22
1.0 1.02 0.28 0.30
FUT5
FUT1
FUT2
FUT4
FUT7
FUT8
random
siRNA1
siRNA2
Supplementary Figure 3. Suppression of FUT3 and FUT6 inhibits Smad2 phosphorylation upon TGF-ß stimulation
siFUT5siFUT1 siFUT2 siFUT4 siFUT7 siFUT8NT Random
TGF-β – –+ + – + – + – + – –+ + – +
pSmad2
Smad2
A
B
1.0 0.26 0.28
1.0 0.36 0.32
1.0 0.21 0.22
1.0 0.44 0.72
1.0 0.33 0.43
1.0 0.27 0.35
1.0 1.36 1.0 1.25 1.0 3.17 1.0 1.38 1.0 1.33 1.0 1.42 1.0 1.52 1.0 1.35
Supplementary Figure 4. Fucosylation of TßR-I was not affected by TGF-ß stimulation
IgG 0 6 12
TGF-β (hr)
TßR-I IP/ AAL IB
TßR-I
TßR-I IP/ TßRI IB TßR-I
1.00 1.05 1.04