supplementary figure 1. morphological...

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15 3 10 5 7 12 8 4 9 14 11 13 1 2 6 26 24 25 21 20 23 22 27 29 28 32 30 31 19 18 17 16 Nasohypophysial Slanting gills Cranium cart* Tectal cart* Pharyngobranchial Pericardiac cart* Oral disc Arcualia* Branchial cart.* Hyoid* Sensory lines Horny “teeth”* Lingual cartilage* Lingual musculature Velum* Gill pouch shape Dorsal fins* Eye lens Radial muscles Gill symmetry Gill lamellae Pineal organ Skull* Brain Otic capsules* Myomere W’s Liver Hypophysis Caudal fin* Kidney Paired eyes Pigment cells Multichamber heart Post-anal tail Myomeres Notochord Nerve chord Pharyngeal arches Oral papillae Annular cart*H Trematic rings* United Atrium/ventricle Lingual cart.* Slime glands Oral tentacles* Nasophyaryngeal duct Prenasal sinus* Subnasal cartilage* Fibrous brain sheath Separated atrium/ventricle Nasal basket* Ethmoid tooth* Dentigerous cart.* Perpendicular muscle cart.* Accesory hearts Oesophagocutaneous duct Gill asymmetry Skull* Brain Otic capsules* Myomere W’s Multichamber heart Liver Hypophysis* Post-anal tail Myomeres Caudal fin* Notochord Dorsal nerve cord Pharyngeal arches* Horny “teeth”* Lingual musculature Velum* Gill pouch shape 17 7.5 7.5 7.5 7.5 7.5 7.5 7.5 7.5 7.5 7.5 7.5 7.5 7.5 7.5 23.5 23.5 23.5 23.5 23.5 23.5 23.5 23.5 30 30 30 30 30 17 17 17 17 15 3 10 5 7 12 8 4 9 14 11 13 1 2 6 26 24 25 21 20 23 22 27 29 28 32 30 31 19 18 17 16 MYXINOIDEA CYCLOST. CRANIATA CHORDATA 18.5 18.5 18.5 18.5 18.5 5.5 5.5 5.5 5.5 18.5 5.5 5.5 5.5 18.5 18.5 18.5 5.5 5.5 5.5 18.5 18.5 18.5 18.5 18.5 18.5 18..5 32 32 32 32 32 32 18.5 18.5 18.5 18.5 32 32 32 32 32 40 40 40 40 40 35 36 34 15 3 10 5 7 12 8 4 9 14 11 13 1 2 6 26 24 25 21 20 23 22 27 29 28 32 30 31 19 18 17 16 37 35 36 34 33 38 40 39 41 42 VERTEBRATA CYCLOSTOMATA CRANIATA CHORDATA Nasohypophysial Slanting gills Cranium cart.* Tectal cart* Pharyngobranchial duct Pericardiac cart.* Oral disc Arcualia* Branchial cart.* Hyoid* Sensory lines Horny “teeth”* Lingual cart.* Lingual musculature Velum* Gill pouch shape Dorsal fins* Eye lens Radial muscles Gill symmetry Gill lamellae Pineal organ Skull* Brain Otic capsules* Myomere W’s Liver Hypophysis Caudal fin* Kidney Paired eyes Pigment cells Multichamber heart Post-anal tail Myomeres Notochord Dorsal nerve cord Pharyngeal arches Oral papillae Annular cart.*H Trematic rings* United atrium/ventricle PETROMYZONTIDA (a) (b) Supplementary figure 1. Morphological synapomorphies of lamprey (a) and hagfish (b) resolved onto phylogeny (figure 1). Colours represent the different hierarchical levels of catergories of synapomorphies e.g. more plesiomorphic characters diagnosing Chordata are green, whilst synapomorphic characters diagnosing the narrower clades of Petromyzontida and Myxinoidea are purple. Numerical synapomorphic ranks (numbers right) are calculated from the mean value of ordinal ranks (numbers left) for each catergory. The dashed lines in the lamprey phylogeny (a) represent the alternative positions of Petromyzontida (i.e. cyclostome paraphyly on the left branch and cyclostome monophyly on the right branch). Asterisks mark morphological characters with skeletal components.

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15

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11

13

12

6

26

2425

2120

2322

27

2928

32

3031

19181716

Nasohypophysial

Slanting gills

Cranium cart*

Tectal cart*

PharyngobranchialPericardiac cart*

Oral disc

Arcualia*Branchial cart.*

Hyoid*Sensory lines

Horny “teeth”*Lingual cartilage*

Lingual musculatureVelum*

Gill pouch shape

Dorsal �ns*Eye lens

Radial musclesGill symmetry

Gill lamellaePineal organ

Skull*Brain

Otic capsules*

Myomere W’s

Liver

Hypophysis

Caudal �n*

Kidney

Paired eyes

Pigment cells

Multichamber heart

Post-anal tailMyomeresNotochord

Nerve chordPharyngeal arches

Oral papillaeAnnular cart*H

Trematic rings*

United Atrium/ventricle

Lingual cart.*

Slime glands

Oral tentacles*

Nasophyaryngeal duct

Prenasal sinus*

Subnasal cartilage*

Fibrous brain sheath

Separated atrium/ventricle

Nasal basket*

Ethmoid tooth*

Dentigerous cart.*

Perpendicular muscle cart.*

Accesory heartsOesophagocutaneous duct

Gill asymmetry

Skull*

BrainOtic capsules*

Myomere W’s

Multichamber heart

Liver

Hypophysis*

Post-anal tailMyomeres

Caudal �n*

Notochord

Dorsal nerve cordPharyngeal arches*

Horny “teeth”*Lingual musculatureVelum*Gill pouch shape17

7.5

7.5

7.5

7.5

7.5

7.5

7.5

7.5

7.5

7.5

7.5

7.57.5

7.5

23.5

23.523.5

23.523.5

23.523.5

23.5

3030

30

3030

17171717

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MYX

INO

IDEA

CYCL

OST

.

CRAN

IATA

CHO

RDAT

A

18.518.5

18.518.5

18.5

5.5

5.5

5.5

5.5

18.5

5.5

5.5

5.5

18.5

18.5

18.5

5.55.5

5.5

18.5

18.518.5

18.518.5

18.518..5

32

3232

32

3232

18.518.5

18.518.5

32

3232

3232

40

4040

4040

3536

34

15

3

10

5

7

12

8

4

9

14

11

13

12

6

26

2425

2120

2322

27

2928

32

3031

1918

1716

37

3536

3433

38

4039

4142

VERTEBRATA

CYCL

OST

OM

ATA

CRAN

IATA

CHO

RDAT

A

Nasohypophysial

Slanting gills

Cranium cart.*

Tectal cart*

Pharyngobranchial ductPericardiac cart.*

Oral disc

Arcualia*Branchial cart.*

Hyoid*Sensory lines

Horny “teeth”*Lingual cart.*

Lingual musculatureVelum*

Gill pouch shape

Dorsal �ns*Eye lens

Radial musclesGill symmetry

Gill lamellaePineal organ

Skull*Brain

Otic capsules*

Myomere W’s

Liver

Hypophysis

Caudal �n*

Kidney

Paired eyes

Pigment cells

Multichamber heart

Post-anal tailMyomeresNotochord

Dorsal nerve cordPharyngeal arches

Oral papillaeAnnular cart.*H

Trematic rings*

United atrium/ventricle

PETR

OM

YZO

NTI

DA

(a) (b)

Supplementary figure 1. Morphological synapomorphies of lamprey (a) and hagfish (b) resolved onto phylogeny (figure 1). Colours represent the different hierarchical levels of catergories of synapomorphies e.g. more plesiomorphic characters diagnosing Chordata are green, whilst synapomorphic characters diagnosing the narrower clades of Petromyzontida and Myxinoidea are purple. Numerical synapomorphic ranks (numbers right) are calculated from the mean value of ordinal ranks (numbers left) for each catergory. The dashed lines in the lamprey phylogeny (a) represent the alternative positions of Petromyzontida (i.e. cyclostome paraphyly on the left branch and cyclostome monophyly on the right branch). Asterisks mark morphological characters with skeletal components.