Slide 1

Supplementary Figure 1 Supplemental Figure 1. Nogin siRNA potentiates BMP2-induced Smad-dependent BMP- signaling MDA-MB-231 breast cancer cells. MDA-MB-231/BREluc cells were transfected with Noggin siRNA or NT siRNA (Dharmacon) 24 hours prior addition of BMPs (50 ng/ml) for 30 hours. $$$ P< 0.001 using two-way ANOVA. Experiment was performed in triplicate. Slide 2 Supplemental Figure 2. Smad3/4-dependent TGF-signaling in MDA-231 breast cancer cells. MDA-MB-231/CAGAluc2 cells were characterized by performing a dose-response with different TGF isoforms. N = 2 independent experiments. No significant differences were observed between the TGF isoforms. ** P