surfactant toxicology...poly-ethoxylated fatty amines n i \ n. oh n lo 0 oh n. oh n lo 0 , oh n ho 0...
TRANSCRIPT
![Page 1: SURFACTANT TOXICOLOGY...Poly-ethoxylated fatty amines N I \ N. OH N lo 0 OH N. OH N lo 0 , OH N HO 0 N 0 OH ' lo OH EX. 0075-7 SURFACTANT TOXICOLOGY Sphingolipids of the cell membrane](https://reader035.vdocument.in/reader035/viewer/2022081620/610bf1279ae9e560bc08b074/html5/thumbnails/1.jpg)
SURFACTANT TOXICOLOGY Mark A. Martens
Toxicology, Europe/Africa
EX. 0075
EX. 0075 - 1
![Page 2: SURFACTANT TOXICOLOGY...Poly-ethoxylated fatty amines N I \ N. OH N lo 0 OH N. OH N lo 0 , OH N HO 0 N 0 OH ' lo OH EX. 0075-7 SURFACTANT TOXICOLOGY Sphingolipids of the cell membrane](https://reader035.vdocument.in/reader035/viewer/2022081620/610bf1279ae9e560bc08b074/html5/thumbnails/2.jpg)
SURFACTANT TOXICOLOGY
Outline
• Toxicity related to surfactant action
• Specific cases of toxicity - Genotoxicity
- Oestrogenicity
EX. 0075 - 2
![Page 3: SURFACTANT TOXICOLOGY...Poly-ethoxylated fatty amines N I \ N. OH N lo 0 OH N. OH N lo 0 , OH N HO 0 N 0 OH ' lo OH EX. 0075-7 SURFACTANT TOXICOLOGY Sphingolipids of the cell membrane](https://reader035.vdocument.in/reader035/viewer/2022081620/610bf1279ae9e560bc08b074/html5/thumbnails/3.jpg)
I
I
SURFACTANT TOXICOLOGY Toxicity related to surfactant action
Skin • Corrosion (tissue destruction)
• Irritation (inflammation)
• Sensitisation (allergy)
• Irritative dermatitis
Eyes • Corrosion (tissue destruction)
• Irreversible corneal lesions (blindness)
• Irritation (inflammation eye lids)
I ntesti n.es • Necrosis
• Inflammation
• Water retention ➔ hypovolemic shock
Kinetics. • Poor skin absorption
• Poor intestinal absorption
• Enhancement of skin absorption of other chemicals
EX. 0075 - 3
![Page 4: SURFACTANT TOXICOLOGY...Poly-ethoxylated fatty amines N I \ N. OH N lo 0 OH N. OH N lo 0 , OH N HO 0 N 0 OH ' lo OH EX. 0075-7 SURFACTANT TOXICOLOGY Sphingolipids of the cell membrane](https://reader035.vdocument.in/reader035/viewer/2022081620/610bf1279ae9e560bc08b074/html5/thumbnails/4.jpg)
SURFACTANT TOXICOLOGY Toxicity related to surfactant action
Common toxicologic mechanism:
EX. 0075- 4
![Page 5: SURFACTANT TOXICOLOGY...Poly-ethoxylated fatty amines N I \ N. OH N lo 0 OH N. OH N lo 0 , OH N HO 0 N 0 OH ' lo OH EX. 0075-7 SURFACTANT TOXICOLOGY Sphingolipids of the cell membrane](https://reader035.vdocument.in/reader035/viewer/2022081620/610bf1279ae9e560bc08b074/html5/thumbnails/5.jpg)
SURFACTANT TOXICOLOGY
Toxicity related to surfactant action
•
·R· • • • 1. ... • •
![Page 6: SURFACTANT TOXICOLOGY...Poly-ethoxylated fatty amines N I \ N. OH N lo 0 OH N. OH N lo 0 , OH N HO 0 N 0 OH ' lo OH EX. 0075-7 SURFACTANT TOXICOLOGY Sphingolipids of the cell membrane](https://reader035.vdocument.in/reader035/viewer/2022081620/610bf1279ae9e560bc08b074/html5/thumbnails/6.jpg)
SURFACTANT TOXICOLOGY Toxicity related to surfactant action
li1pid-·soluble, m1olecules,, 02 ' 1Q,0 12,' H,20
EX. 0075 - 6
![Page 7: SURFACTANT TOXICOLOGY...Poly-ethoxylated fatty amines N I \ N. OH N lo 0 OH N. OH N lo 0 , OH N HO 0 N 0 OH ' lo OH EX. 0075-7 SURFACTANT TOXICOLOGY Sphingolipids of the cell membrane](https://reader035.vdocument.in/reader035/viewer/2022081620/610bf1279ae9e560bc08b074/html5/thumbnails/7.jpg)
SURFACTANT TOXICOLOGY Poly-ethoxylated fatty amines
I N \
OH N. N 0
l o OH
N. OH N 0
l o OH
, HO N 0 N 0 OH
' l o OH
EX. 0075- 7
![Page 8: SURFACTANT TOXICOLOGY...Poly-ethoxylated fatty amines N I \ N. OH N lo 0 OH N. OH N lo 0 , OH N HO 0 N 0 OH ' lo OH EX. 0075-7 SURFACTANT TOXICOLOGY Sphingolipids of the cell membrane](https://reader035.vdocument.in/reader035/viewer/2022081620/610bf1279ae9e560bc08b074/html5/thumbnails/8.jpg)
SURFACTANT TOXICOLOGY
Sphingolipids of the cell membrane
Sphingosine
Ceramide
EX. 0075- 8
![Page 9: SURFACTANT TOXICOLOGY...Poly-ethoxylated fatty amines N I \ N. OH N lo 0 OH N. OH N lo 0 , OH N HO 0 N 0 OH ' lo OH EX. 0075-7 SURFACTANT TOXICOLOGY Sphingolipids of the cell membrane](https://reader035.vdocument.in/reader035/viewer/2022081620/610bf1279ae9e560bc08b074/html5/thumbnails/9.jpg)
SURFACTANT TOXICOLOGY Toxicity related to surfactant action
cell membrane or organelle membranes
EX. 0075 - 9
![Page 10: SURFACTANT TOXICOLOGY...Poly-ethoxylated fatty amines N I \ N. OH N lo 0 OH N. OH N lo 0 , OH N HO 0 N 0 OH ' lo OH EX. 0075-7 SURFACTANT TOXICOLOGY Sphingolipids of the cell membrane](https://reader035.vdocument.in/reader035/viewer/2022081620/610bf1279ae9e560bc08b074/html5/thumbnails/10.jpg)
SURFACTANT TOXICOLOGY
Aggresivity to mucous membranes/skin
Fatty amines
Corrosive
Irritant
Alkalinity
Water solubility
EX. 0075 - 10
![Page 11: SURFACTANT TOXICOLOGY...Poly-ethoxylated fatty amines N I \ N. OH N lo 0 OH N. OH N lo 0 , OH N HO 0 N 0 OH ' lo OH EX. 0075-7 SURFACTANT TOXICOLOGY Sphingolipids of the cell membrane](https://reader035.vdocument.in/reader035/viewer/2022081620/610bf1279ae9e560bc08b074/html5/thumbnails/11.jpg)
SURFACTANT TOXICOLOGY Aggresivity to mucous membranes/skin
I Corrosive and antiseptic
N+
I Quaternary ammonium salts
' N+.
Humectant
' N+
Alkalinity
' Water solubility
EX. 0075 - 11
![Page 12: SURFACTANT TOXICOLOGY...Poly-ethoxylated fatty amines N I \ N. OH N lo 0 OH N. OH N lo 0 , OH N HO 0 N 0 OH ' lo OH EX. 0075-7 SURFACTANT TOXICOLOGY Sphingolipids of the cell membrane](https://reader035.vdocument.in/reader035/viewer/2022081620/610bf1279ae9e560bc08b074/html5/thumbnails/12.jpg)
SURFACTANT TOXICOLOGY Toxicity
Phosphate esters
Water solubility
EX. 0075 - 12
![Page 13: SURFACTANT TOXICOLOGY...Poly-ethoxylated fatty amines N I \ N. OH N lo 0 OH N. OH N lo 0 , OH N HO 0 N 0 OH ' lo OH EX. 0075-7 SURFACTANT TOXICOLOGY Sphingolipids of the cell membrane](https://reader035.vdocument.in/reader035/viewer/2022081620/610bf1279ae9e560bc08b074/html5/thumbnails/13.jpg)
SURFACTANT TOXICOLOGY
Surfact. Type Cyto- Eye Fish 'tQxlc..ity irritation t<>x_ici~y
(ECS'0, (EU class) (LC50, ug/ml) mg/L)
HOET e N. 28 Xi, R41 0.1-1
3329
MON N. 26 Xi, R41 1.3 0818
Dodigen
!♦• 233 < Xi > 500
4022
Tween oe 695 216 20
G3 mix Dodigen 4022 + 2068 < Xi 500-1000 Tween 20
EX. 0075 - 13
![Page 14: SURFACTANT TOXICOLOGY...Poly-ethoxylated fatty amines N I \ N. OH N lo 0 OH N. OH N lo 0 , OH N HO 0 N 0 OH ' lo OH EX. 0075-7 SURFACTANT TOXICOLOGY Sphingolipids of the cell membrane](https://reader035.vdocument.in/reader035/viewer/2022081620/610bf1279ae9e560bc08b074/html5/thumbnails/14.jpg)
SURFACTANT TOXICOLOGY Toxicity related to surfactant action
Health hazard
Corrosion
Irritation
No irritation
Dilution
Efficacy
Optimum efficacy
EX. 0075 - 14
![Page 15: SURFACTANT TOXICOLOGY...Poly-ethoxylated fatty amines N I \ N. OH N lo 0 OH N. OH N lo 0 , OH N HO 0 N 0 OH ' lo OH EX. 0075-7 SURFACTANT TOXICOLOGY Sphingolipids of the cell membrane](https://reader035.vdocument.in/reader035/viewer/2022081620/610bf1279ae9e560bc08b074/html5/thumbnails/15.jpg)
SURFACTANT TOXICOLOGY Specific toxicity cases: DNA adducts
Peluso et al., 1998:
Increase of DNA adducts in liver and kidneys of mice after intraperitoneal injection (l.p.) of Italian Roundup (MON 35050, not anymore commercial) dissolved in
DMSO/olive oil
This in-vivo genotoxicity finding was cause of concern to regulatory authorities
EX. 0075 - 15
![Page 16: SURFACTANT TOXICOLOGY...Poly-ethoxylated fatty amines N I \ N. OH N lo 0 OH N. OH N lo 0 , OH N HO 0 N 0 OH ' lo OH EX. 0075-7 SURFACTANT TOXICOLOGY Sphingolipids of the cell membrane](https://reader035.vdocument.in/reader035/viewer/2022081620/610bf1279ae9e560bc08b074/html5/thumbnails/16.jpg)
SURFACTANT TOXICOLOGY Specific toxicity cases: DNA adducts
To better understand the significance of these findings Monsanto undertook research to examine the role of:
• The route of administration (l.p. vs oral)
• The vehicle (DMSO/olive oil vs saline)
• The surfactant (MON 35050 with and without glyphosate)
EX. 0075 - 16
![Page 17: SURFACTANT TOXICOLOGY...Poly-ethoxylated fatty amines N I \ N. OH N lo 0 OH N. OH N lo 0 , OH N HO 0 N 0 OH ' lo OH EX. 0075-7 SURFACTANT TOXICOLOGY Sphingolipids of the cell membrane](https://reader035.vdocument.in/reader035/viewer/2022081620/610bf1279ae9e560bc08b074/html5/thumbnails/17.jpg)
SURFACTANT TOXICOLOGY
Specific toxicity cases: DNA adducts Liver toxicity in the CD-1 mouse:
influence of surfactant and vehicle after I.P. administration
Liver enzyme activity ,;;f_,, Ii
,:,:-·
~~~
BUN SDH
-=DMS0/00 + 35050 - gl~ DMS0/00 + 35050
Saline + 35050 DMS0/00
EX. 0075 - 17
![Page 18: SURFACTANT TOXICOLOGY...Poly-ethoxylated fatty amines N I \ N. OH N lo 0 OH N. OH N lo 0 , OH N HO 0 N 0 OH ' lo OH EX. 0075-7 SURFACTANT TOXICOLOGY Sphingolipids of the cell membrane](https://reader035.vdocument.in/reader035/viewer/2022081620/610bf1279ae9e560bc08b074/html5/thumbnails/18.jpg)
SURFACTANT TOXICOLOGY Specific toxicity cases: DNA adducts
2500
2000
;1 500
;1 000
500
0
Liver toxicity in the CD-1 mouse: influence of route of administration
Al!:1i AS1i l!DH
EX. 0075 - 18
![Page 19: SURFACTANT TOXICOLOGY...Poly-ethoxylated fatty amines N I \ N. OH N lo 0 OH N. OH N lo 0 , OH N HO 0 N 0 OH ' lo OH EX. 0075-7 SURFACTANT TOXICOLOGY Sphingolipids of the cell membrane](https://reader035.vdocument.in/reader035/viewer/2022081620/610bf1279ae9e560bc08b074/html5/thumbnails/19.jpg)
SURFACTANT TOXICOLOGY Specific toxicity cases: DNA adducts
Mechanism
Peritoneal cavity
Surfactant penetrates peritoneum and liver capsule
P.eritoneum
White precipitations with surfactant
t iver. caR_sule
EX. 0075 - 19
![Page 20: SURFACTANT TOXICOLOGY...Poly-ethoxylated fatty amines N I \ N. OH N lo 0 OH N. OH N lo 0 , OH N HO 0 N 0 OH ' lo OH EX. 0075-7 SURFACTANT TOXICOLOGY Sphingolipids of the cell membrane](https://reader035.vdocument.in/reader035/viewer/2022081620/610bf1279ae9e560bc08b074/html5/thumbnails/20.jpg)
SURFACTANT TOXICOLOGY
Specific toxicity cases: DNA adducts Conclusions of MON 35050 case
• The I.P. route is an inappropriate route of administration
• The vehicle (DMSO/olive oil) produces precipitates onto the peritoneal membrane with very high local concentrations of surfactant as a consequence ➔ inflammation in underlying organs!
• The surfactant (alkyl sulphate) is the cause of the oxidative damage of DNA in liver and kidneys and not glyphosate
• The observation of the EU/BBA on the Peluso et al. paper was: " ... some indications of DNA damage have been observed ... rather due to cytotoxic properties of the formulation ... "
EX. 0075 - 20
![Page 21: SURFACTANT TOXICOLOGY...Poly-ethoxylated fatty amines N I \ N. OH N lo 0 OH N. OH N lo 0 , OH N HO 0 N 0 OH ' lo OH EX. 0075-7 SURFACTANT TOXICOLOGY Sphingolipids of the cell membrane](https://reader035.vdocument.in/reader035/viewer/2022081620/610bf1279ae9e560bc08b074/html5/thumbnails/21.jpg)
SURFACTANT TOXICOLOGY
Specific toxicity cases: Oestrogenicity
Activation througti biodegradation in disctiarge wate r. treatment stations
Surfactant
Oestrogen.
EX. 0075 - 21
![Page 22: SURFACTANT TOXICOLOGY...Poly-ethoxylated fatty amines N I \ N. OH N lo 0 OH N. OH N lo 0 , OH N HO 0 N 0 OH ' lo OH EX. 0075-7 SURFACTANT TOXICOLOGY Sphingolipids of the cell membrane](https://reader035.vdocument.in/reader035/viewer/2022081620/610bf1279ae9e560bc08b074/html5/thumbnails/22.jpg)
SURFACTANT TOXICOLOGY
Specific toxicity cases: Oestrogenicity
17~-oestradiol 4-nonyl phenol
Oestrogen receptor
EX. 0075 - 22
![Page 23: SURFACTANT TOXICOLOGY...Poly-ethoxylated fatty amines N I \ N. OH N lo 0 OH N. OH N lo 0 , OH N HO 0 N 0 OH ' lo OH EX. 0075-7 SURFACTANT TOXICOLOGY Sphingolipids of the cell membrane](https://reader035.vdocument.in/reader035/viewer/2022081620/610bf1279ae9e560bc08b074/html5/thumbnails/23.jpg)
SURFACTANT TOXICOLOGY
Specific toxicity cases: Oestrogenicity
Vitellogenin production in trout Feminisation of male trout
0 0 0
EX. 0075 - 23
![Page 24: SURFACTANT TOXICOLOGY...Poly-ethoxylated fatty amines N I \ N. OH N lo 0 OH N. OH N lo 0 , OH N HO 0 N 0 OH ' lo OH EX. 0075-7 SURFACTANT TOXICOLOGY Sphingolipids of the cell membrane](https://reader035.vdocument.in/reader035/viewer/2022081620/610bf1279ae9e560bc08b074/html5/thumbnails/24.jpg)
BBP
DBP
NP
SURFACTANT TOXICOLOGY
Specific toxicity cases: Oestrogenicity
In-vitro In-vivo
Recept. Prolifer. Transcript. Uterotr. Hershbinding berger
ER ER
ER ER
ER ER
ER
ER
ER
Peripu-1 bertal
Peripubertal
Reprotox.
2-gen
Sem. Ves.
Penis (F1)
Vaginal
opening
EX. 0075 - 24
![Page 25: SURFACTANT TOXICOLOGY...Poly-ethoxylated fatty amines N I \ N. OH N lo 0 OH N. OH N lo 0 , OH N HO 0 N 0 OH ' lo OH EX. 0075-7 SURFACTANT TOXICOLOGY Sphingolipids of the cell membrane](https://reader035.vdocument.in/reader035/viewer/2022081620/610bf1279ae9e560bc08b074/html5/thumbnails/25.jpg)
SURFACTANT TOXICOLOGY
Specific toxicity cases: Oestrogenicity
Conclusions of the case the polyethoxylated nonyl phenol surfactants (1)
• Polyethoxylated nonyl phenol (and octyl phenol, decyl phenol, undeceyl phenol, dodecyl phenol) surfactants biodegrade to form oestrogenic chemicals (mimic female hormones).
• Nonyl phenol has been shown to be oestrogenic in mammals in-vitro and in-vivo, however, the potency is approx 1,000-10,000 times lower than that of natural oestradiol.
• Nonyl phenol has been shown in-vitro, in vivo and in the field that it can feminise fish at environmentally relevant concentrations
EX. 0075 - 25
![Page 26: SURFACTANT TOXICOLOGY...Poly-ethoxylated fatty amines N I \ N. OH N lo 0 OH N. OH N lo 0 , OH N HO 0 N 0 OH ' lo OH EX. 0075-7 SURFACTANT TOXICOLOGY Sphingolipids of the cell membrane](https://reader035.vdocument.in/reader035/viewer/2022081620/610bf1279ae9e560bc08b074/html5/thumbnails/26.jpg)
SURFACTANT TOXICOLOGY
Specific toxicity cases: Oestrogenicity
Conclusions of the case the polyethoxylated nonyl phenol surfactants (2)
• An effort will have to be done by the pesticide industry to avoid using these surfactants in new formulations and whenever practically possible to replace them in existing formulations
• Monsanto Europe successfully undertook research to find a suitable replacement for po/yethoxylated nonyl phenols. The new surfactant mix has similar technical properties and does not biodegrade into oestrogenic molecular species. It has already been proposed to replace surfactants in trial/ate formulations.
EX. 0075 - 26
![Page 27: SURFACTANT TOXICOLOGY...Poly-ethoxylated fatty amines N I \ N. OH N lo 0 OH N. OH N lo 0 , OH N HO 0 N 0 OH ' lo OH EX. 0075-7 SURFACTANT TOXICOLOGY Sphingolipids of the cell membrane](https://reader035.vdocument.in/reader035/viewer/2022081620/610bf1279ae9e560bc08b074/html5/thumbnails/27.jpg)
SURFACTANT TOXICOLOGY
General conclusions
• Surfactants are biologically not "inert", they can be toxic and this must be addressed
• Part of the toxicity of surfactants is related to the surfactant action which destabilises cell membranes
• Part of the toxicity of surfactants can be specific (skin sensitisation, oestrogenicity)
• The toxicity of surfactants depends of their concentration in the formulation
• The high added value of herbicide formulations containing surfactants resides in the optimal compromise between efficacy and safety for man and the environment
EX. 0075 - 27