symptom score unit
DESCRIPTION
Supplementary material Figure 1. Histograms showing the distributions of SBCMV-response traits in the Meridiano × Claudio (MxC) recombinant inbred line population (181 RILs) and in the durum wheat germplasm panel (111 elite accessions). - PowerPoint PPT PresentationTRANSCRIPT
Supplementary material Figure 1. Histograms showing the distributions of SBCMV-response traits in the Meridiano × Claudio (MxC) recombinant inbred line population (181 RILs) and in the durum wheat germplasm panel (111 elite accessions).Distributions are reported for four field trials carried out in Northern Italy (Po valley) under high disease pressure. The M×C data refer to field experiments carried out at Cadriano (44°35’N 11°27’E) in 2007 and 2008 while the germplasm panel data refer to field experiments carried out at Minerbio (44°37’N 11°29’E ) in 2003 and 2004.The SBCMV-response traits considered were: symptom severity (SS) visually scored following a 0-4 scale and leaf virus concentration determined using DAS-ELISA and reported as absorbance units at 405 nm.
Symptom scoring and leaf sampling were carried out on four dates in the M×C RIL experiments and on two dates in the germplasm panel experiments at the plant growth stages showing the maximum disease symptom intensity, i.e. from mid-end of tillering (Z25 in the Zadoks scale, Zadoks et al., 1974) to first (Z31) and second (Z32) node appearance (corresponding to a period from the end of February to April).
Mean values for the RILs (M×C), Meridiano (M), Claudio (C) and for the highly susceptible check cv. Grazia (G) are indicated with arrows and reported together with the corresponding standard deviation in parenthesis.
Symptom score unit
RIL
nu
mb
er
Symptom score unit
RIL
nu
mb
er
Symptom score unit
Symptom score unit
100
MxC RIL SS2-07
0 1 2 3 4
10
20
30
40
50
60
70
80
90
100
1 2
MxC RIL SS3-07
0 3 4
10
20
30
40
50
60
70
80
90
100
RIL
nu
mb
er
MxC RIL SS4-07
0 1 2 3 4
10
20
30
40
50
60
70
80
90
RIL
nu
mb
er
MxC RIL SS1-07
0 1 2 3 4
10
20
30
40
50
60
70
80
90
70
RIL
(1.
3)
100
C (
1.9
± 0.
7)
M (
0.1
± 0.
1)
G (
3.2
± 0.
3)
RIL
(1.
4)
C (
2.1
± 0.
5)
M (
0.1
± 0.
1)
G (
3.1
± 0.
2)
RIL
(1.
6)
C (
2.7
± 0.
3)
M (
0.3
± 0.
3)
G (
3.8
± 0.
3)
RIL
(1.
2)
C (
2.5
± 0.
3)
M (
0.1
± 0.
1)
G (
3.1
± 0.
2)
MxC RIL ELISA2-07 MxC RIL ELISA4-07
210.5 1.50
10
20
30
40
50
60
210.5 1.50
10
20
30
40
50
60
Absorbance units (A 405 nm)
RIL
nu
mb
er
RIL
nu
mb
er
Absorbance units (A 405 nm)
RIL
(1.
2)
M (
0.8
± 0.
5)
G (
1.5
± 0.
3)
C (
1.4
± 0.
3)
RIL
(1.
4)
M (
1.2
± 0.
6)
G (
1.9
± 0.
2)
C (
1.8
± 0.
2)
MxC RIL SS1-08
MxC RIL SS2-08 MxC RIL SS4-08
0 1 2 3 4
10
20
30
40
50
60
70
80
90
100
1 2
0 1 2 3 4
0 1 2 3 4
10
20
30
40
50
60
70
80
90
100
10
20
30
40
50
60
70
80
90
100
Symptom score unit
RIL
nu
mb
er
MxC RIL SS3-08
0 3 4
10
20
30
40
50
60
70
80
90
100
Symptom score unit
RIL
nu
mb
er
Symptom score unit
RIL
nu
mb
er
RIL
nu
mb
er
Symptom score unit
RIL
(1.
5)
G (
3.5
± 0.
3)
C (
2.2
± 0.
5)
M (
0.3
± 0.
4)
RIL
(1.
7)
G (
3.5
± 0.
3)
C (
2.3
± 0.
5)
M (
0.2
± 0.
0)
RIL
(1.
3)
G (
3.6
± 0.
3)
C (
1.8
± 0.
7)
M (
0.0
± 0.
0)
RIL
(0.
6)
G (
2.0
± 0.
9)
C (
0.4
± 0.
4) M
(0.
0 ±
0.0)
10
20
30
40
50
60MxC RIL ELISA2-08
210.5 1.50
Absorbance units (A 405 nm)
RIL
nu
mb
er
MxC RIL ELISA4-08
10
20
30
40
50
60
Absorbance units (A 405 nm)
RIL
nu
mb
er
RIL
(1.
2)
G (
2.0
± 0.
0)
C (
1.6
± 0.
6)
M (
0.4
± 0.
7)
RIL
(1.
1)
M (
0.3
± 0.
4)
210.5 1.50
C (
1.9
± 0.
2)
G (
2.0
± 0.
0)
Durum Panel SS1-03
Durum Panel SS2-03
0 1 2 3 4
0 1 2 3 4
20
30
50
10
40
10
20
30
40
50
Durum Panel SS2-04
0 1 2 3 4
10
20
30
40
50
Symptom score unit
Durum Panel SS1-04
0 1 2 3 4
10
20
30
40
50A
cce
ssio
n
nu
mb
er
Acc
ess
ion
n
um
be
r
Symptom score unit
Symptom score unit
Acc
ess
ion
n
um
be
rA
cce
ssio
n
nu
mb
er
Symptom score unit
G (
2.7
± 0.
2)
G (
2.8
± 0.
2)
G (
3.3
± 0.
3)
G (
2.7
± 0.
0)
Durum Panel ELISA1-03
210.5 1.50
10
20
30
40
50
60
Durum Panel ELISA2-03
10
20
30
40
50
60
Durum Panel ELISA1-04
Durum Panel ELISA2-04
10
20
30
40
50
60
10
20
30
40
50
60Absorbance units (A 405 nm)
Acc
ess
ion
nu
mb
er
Acc
ess
ion
nu
mb
er
210.5 1.50
210.5 1.50 210.5 1.50
Absorbance units (A 405 nm)
Absorbance units (A 405 nm)
Absorbance units (A 405 nm)
G (
1.4
± 0.
2)
G (
1.7
± 0.
0)
G (
1.4
± 0.
1)
G (
1.8
± 0.
0)