t1 and t2
DESCRIPTION
Lee et al. Supp Fig 1. B. A. C. T1 and T2. T3 and T4. Lee et al. Supp Fig 2. LNCaP. 22RV1. Lee et al. Supp Fig 3. B. A. *. *. *. *. *. *. *. C. *. Lee et al. Supp Fig 4. BMPRIa. ActRIIa. Smad1. Smad5. ActRIIb. BMPRIb. Smad8. ActRIa. BMPRII. β -actin. - PowerPoint PPT PresentationTRANSCRIPT
Bone meta (-) Bone meta (+)
n=101 n=57 p-value
Age (min-max) 73.0 (51-88) 71.0 (55-86) 0.027BMI (min-max)PrePSA (min-
max)
24.0 (16.0-32)20.0 (2.0-
982.0)
23.0 (17-28)139.0 (7.8-
6262.0)
0.0370.001
Gleason score 0.006≤6 24 (23.8) 3 (5.3)7 29 (28.7) 15 (26.3)
≥8 48 (47.5) 39 (68.4)Posttreatment
PSA nadir0.04 (0.01-
30.4)0.2 (0.01-79.8) 0.04
A.
C.
B.
T1 and T2 T3 and T4
Lee et al. Supp Fig 1
LNCaP 22RV1
Lee et al. Supp Fig 2
Lee et al. Supp Fig 3
A.*
0
50
100
150
200
250
Con BMP-6 1ng/ml BMP-610ng/ml
Cel
l co
un
ts (
% o
f co
n)
1d 2d 3d 4d
** *
**
*
B.
0
50
100
150
200
22RV1+WPMY-1 22RV1+HS-5
Ce
ll c
ou
nts
(%
of
co
n)
IgG BMP-6 Ab
C.
*
BMPRII
ActRIIa
ActRIIb
β-actin
Con
siRN
A
BM
PR
II siR
NA
ActR
IIa siR
NA
ActR
IIb siR
NA
BMPRIa
BMPRIb
ActRIa
β-actin
Con
siRN
A
BM
PR
Ia siR
NA
BM
PR
Ib siR
NA
ActR
Ia siR
NA
Smad1
Smad5
Smad8
β-actin
Con
siRN
A
Sm
ad1 siR
NA
Sm
ad5 siR
NA
Sm
ad8 siR
NA
Lee et al. Supp Fig 4
Wnt5a
β-actin
LNCaP 22RV1
WPM
Y-1
HS-5
WPM
Y-1
H-5Stromal
Cell line- - --
Lee et al. Supp Fig 5
Lee et al. Supp Fig 6
22RV1
SP600125:10uM, Calphostin C:100nM
Wnt5a - + + +
Inhibitor - -
SP60
012
5
Calp
hosti
n C
JNKi PKCi
0
1
2
3
4
5
6
7
8
9
Fo
ld in
cre
as
e
Wnt5a - + + +
Inhibitor - -
SP60
012
5
Calp
hosti
n C
JNKi PKCi
BMP-6
-actin
Lux
Lux
Lux
Lux
Lux
Lux
ATG
TATA CATNF-B
0 1 2 3 4
pGL3
0.3kb
0.6kb
0.9kb
1.2kb
1.5kb
BMP-6 Lux activity (Fold)
Lee et al. Supp Fig 7
PCKNF-B receptor
Lee et al. Supp Fig 8