table s1. primers used in mutagenesis of q motif of fancj and blm

2

Primers Sequence (5’-3’) FANCJ-Q25AF GCTTACCCGTCAGCGCTTGCTATGATG FANCJ-Q25AR CATCATAGCAAGCGCTGACGGGTAAGC FANCJ-QF GTGGGGTGAAGATTTACCTTGCTATGATGAATTC FANCJ-QR GAATTCATCATAGCAAGGTAAATCTTCACCCCAC BLM-Q672RF CATAATTTTAGAACTAATCGGCTAGAGGCGATCAATGC BLM-Q672RR GCATTGATCGCCTCTAGCCGATTAGTTCTAAAATTATG BLM-Q672AF CATAATTTTAGAACTAATGCGCTAGAGGCGATCAATGC BLM-Q672AR GCATTGATCGCCTCTAGCGCATTAGTTCTAAAATTATG TABLE S1. Primers used in mutagenesis of Q motif of FANCJ and BLM

Upload: cheyenne-frazier

Post on 02-Jan-2016

33 views

Category:

Documents

1 download

Report

Download

Embed Size (px):

DESCRIPTION

TABLE S1. Primers used in mutagenesis of Q motif of FANCJ and BLM. TABLE S2. DNA substrates or oligonucleotides used in this study. 5’. 26. DC26:TTTTTTTTTTTTTTTTTTTTTTCCCAGTAAAACGACGGCCAGTGC T STEM 25:GCGGTCCCAAAAGGGTCAGTGCTGGCATTTTGCTGCCGGTCACG. I I I I I I I I I I I I I I I I I I I. - PowerPoint PPT Presentation

TRANSCRIPT

Page 1: TABLE S1. Primers used in mutagenesis of Q motif of FANCJ and BLM

Primers Sequence (5’-3’)

FANCJ-Q25AF GCTTACCCGTCAGCGCTTGCTATGATG

FANCJ-Q25AR CATCATAGCAAGCGCTGACGGGTAAGC

FANCJ-QF GTGGGGTGAAGATTTACCTTGCTATGATGAATTC

FANCJ-QR GAATTCATCATAGCAAGGTAAATCTTCACCCCAC

BLM-Q672RF CATAATTTTAGAACTAATCGGCTAGAGGCGATCAATGC

BLM-Q672RR GCATTGATCGCCTCTAGCCGATTAGTTCTAAAATTATG

BLM-Q672AF CATAATTTTAGAACTAATGCGCTAGAGGCGATCAATGC

BLM-Q672AR GCATTGATCGCCTCTAGCGCATTAGTTCTAAAATTATG

TABLE S1. Primers used in mutagenesis of Q motif of FANCJ and BLM

Page 2: TABLE S1. Primers used in mutagenesis of Q motif of FANCJ and BLM

DNA substrate/Oligonucleotides

Sequence (5’-3’)

Forked duplex

TP-G4 TP: TGGACCAGACCTAGCAGCTATGGGGGAGCTGGGGAAGGTGGGAATGTGA

X12-1-52-BIOT13 TTTTTTTTTTTTTTTTGACGCTGCCGAATTCTGGCTTGCTAGGACATCTTTG-BiodT-CCACGTTGACCCG

45-mer (DC26) TTTTTTTTTTTTTTTTTTTTTTCCCAGTAAAACGACGGCCAGTGC

DC26: TTTTTTTTTTTTTTTTTTTTTTCCCAGTAAAACGACGGCCAGTGC

TSTEM25: GCGGTCCCAAAAGGGTCAGTGCTGGCATTTTGCTGCCGGTCACGI I I I I I I I I I I I I I I I I I I

5’

3’

TABLE S2. DNA substrates or oligonucleotides used in this study

BiodT represents an internal biotin conjugated dT.

26

19 bp

5’

3’ 25

Nombres Primers

Mutagenesis Poster

Futures Primers

Transposon mutagenesis in Mycoplasma …researchonline.lshtm.ac.uk/1440259/1/1297-9716-44-124.pdf(primers Tpn1 and Tpn2), introducing anXhoIcutsite at the 3’ end and NdeIandNcoI

Supplementary Materials for - Science...polymerases) and targeted random spike mutagenesis (5-10%) of conserved residues. Overlapping primers harboring a BsaI site in their 5'-terminus,

Genome-Scale Mutagenesis

Site-directed Mutagenesis

SUPPLEMENTARY INFORMATION - media.nature.com was mutated to AGGA, was created by Quikchange mutagenesis (Stratagene) using the primers MBPY17AGGAf and MBPY17AGGAr

ARDEX Primers ARDEX PRIMERS...ARDEX Primers AF 133 11/14 Key Features ARDEX PRIMERS • Solvent-free • Concentrated primer - dilutes with water • Seals the substrate • Interior

Site Directed Mutagenesis

1 Directed Mutagenesis and Protein Engineering. 2 Mutagenesis Mutagenesis -> change in DNA sequence -> Point mutations or large modifications Point mutations

Primers Fundamento

Genomes and mutagenesis

Thaumarchaeota Primers

Tnt1 Retrotransposon Mutagenesis: A Tool for Soybeanparrottlab.uga.edu/parrottlab/Mutagenesis/tnt/Cuietal2011.pdf · Tnt1 Retrotransposon Mutagenesis: A Tool for Soybean Functional

FANCJ regulates the stability of FANCD2/FANCI proteins and ...Map of FANCJ functional domains, protein interaction sites and mutations. This figure illustrates the various domains

Study and engineering of gene function: mutagenesis I. Why mutagenize? II. Random mutagenesis, mutant selection schemes III. Site-directed mutagenesis,

Firearms Auction Saturday, June 22nd, 2019 Listing...Pistol Primers (approx. 3000); Small Rifle Primers (approx. 2000) & Small Pistol Primers (approx. 6000) 27 Large Rifle Primers

Mutagenesis Techniques (PDF)

A fluorescence quenching assay to discriminate between ... et al S3 Table S1. Sequences of oligonucleotide primers used for site-directed mutagenesis. Primer Sequence W5A-FOR 5’-GGAGTATTGGCG

Site-Directed Mutagenesis Gene Staphylococcus ofHistidines ...Site-directed mutagenesis and transformations. By using oligonucleotide-directed mutagenesis, the codon for each of the

PickMutant® Site -directed Mutagenesis Kit€¦ · PickMutant Site -directed Mutagenesis kit is a based -PCR mutagenesis method so the design of primers is essential to obtain the

Transposons Mediated Mutagenesis

Insertional Mutagenesis Strategies

(12) United States Patent Fields et al. (45) Date of ... · Gillam and Smith, “Site-Specific Mutagenesis. Using Synthetic Oligodeoxyribonucleotide Primers: I. Optimum Conditions

FANCJ promotes DNA synthesis through …...Article FANCJ promotes DNA synthesis through G-quadruplex structures Pau Castillo Bosch1, Sandra Segura-Bayona1, Wouter Koole2, Jane T van

FANCJ suppresses microsatellite instability and ...genesdev.cshlp.org/content/29/24/2532.full.pdf · FANCJ suppresses microsatellite instability and lymphomagenesis independent of

transposon mediated mutagenesis

QTL analysis / Mutagenesis

Technology primers

AMERICAN PRIMERS