tajuddin.pdf

7/29/2019 Tajuddin.pdf

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Mass Propagation ofMass Propagation of

JatrophaJatropha curcascurcas L. usingL. using

Ex VitroEx Vitro CultureCultureTeuku Tajuddin,Teuku Tajuddin, MinaldiMinaldi, Linda, Linda NovitaNovita,,YenniYenni BakhtiarBakhtiar andand NadirmanNadirman HaskaHaska

BIOTECHNOLOGY CENTER, BPPTFebruary 2009

INTRODUCTIONINTRODUCTION A total area of the marginal, critical andA total area of the marginal, critical and

degraded land in Indonesia is around 22degraded land in Indonesia is around 22m on ectares. Concern ng t em on ectares. Concern ng t eenvironmental issues, conservationenvironmental issues, conservation

purpose of land remediation.purpose of land remediation.

overnmenovernmen asassuggested that thesuggested that the

area of Jatrophaarea of Jatropha

Ha.Ha.

7.5 billion seedling.7.5 billion seedling.


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OBSTACLESOBSTACLES

Superior mother plants are rareSuperior mother plants are rare

Propagation of seedling in largePropagation of seedling in largeamountamount difficultdifficult

MicroMicro--propagation using tissuepropagation using tissue

Transportation of seedlingTransportation of seedling --expens veexpens ve

Plant needs some eriods of timePlant needs some eriods of timefor adaptation in new environmentfor adaptation in new environment

generativelygeneratively andand vegetativelyvegetatively..

sexuallysexually byby seeds,seeds, whilewhile vegetativevegetative methodmethod byby,, ,,

andand inin vitrovitro cultureculture..

ee p an sp an s propaga epropaga e yy see ssee s areare g yg yheterozygousheterozygous.. Thus,Thus, variationsvariations inin geneticgenetic

InIn vegetativevegetative propagation,propagation, unlikeunlike generativegenerative,,

plantsplants withwith respectrespect toto yield,yield, oiloil qualityquality andanddiseasedisease resistancesresistances areare conservedconserved inin theirtheirprogeniesprogenies..


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PROBLEM SOLVINGPROBLEM SOLVING

Propagation method for seedling productionPropagation method for seedling productionas o e as , nexpens ve, easy an gas o e as , nexpens ve, easy an g

capacitycapacity Ideally seedling production on or nearIdeally seedling production on or near

plantation / planting areaplantation / planting area no transportationno transportationnee enee e

Mother lants are selected from the area whereMother lants are selected from the area wherethe plantation take placethe plantation take place no adaptationno adaptation

process with their environmentprocess with their environment BioBio--remediation on crit ical lands by applicationremediation on critical lands by application

of biotechnolo roductsof biotechnolo roducts

Biotech Center BPPT has developedBiotech Center BPPT has developedanot er met o o vegetat veanot er met o o vegetat vepropagation on some woody plant,propagation on some woody plant,nc u ngnc u ng Ja t r o p h a cu r c a s Ja t r o p h a cu r c a s , w c s, w c sknown as anknown as an e x v i t r o e x v i t r o culture.culture.

ThisThis method is adopted frommethod is adopted fromn v r o n v r o --

propagationpropagation system, whichsystem, which

vessels.vessels.


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METHODOLOGYMETHODOLOGY

InIn e x v i t r o e x v i t r o method, rootingmethod, rooting

directly from thedirectly from the

apical shoots ofapical shoots ofp an s, a erp an s, a ersomesometreatments, andtreatments, andthen producingthen producingnew plantlets.new plantlets.

e p an ee p an ederived fromderived from

this method canthis method candirectly bedirectly beacclimatizedacclimatized

house.house.

e x v r o e x v r o lowlow--cost and simple method to propagatecost and simple method to propagate

plantlet produced fromplantlet produced from i n v i t r o i n v i t r o micromicro--

conditions areconditions are

regards to physicalregards to physical

nutritional andnutritional andhormonal factors.hormonal factors.


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ItIt cancan bebe produceproduce inin aa largelarge amountamount ofof,,

developmentdevelopment asas theirtheir mothermother plantplant..

mu a ngmu a ng agen sagen s ormu a eormu a e yy ee

scientistsscientists inin BiotechBiotech Center,Center, BPPT,BPPT, specialspecial forfor

JatropexJatropex::

for apical shootsfor apical shoots

production onproduction on

mother plantsmother plants

JatrosinJatrosin: sterilant: sterilant JatrodixJatrodix: root: root

induction agentinduction agent

JatrofertJatrofert::

stimulating growthstimulating growth

ex-mining, critical or

planting oil- producing

2. Bio-remediation on critical land b a lication

of soil-borne fungus Mikoriza arbuskular

. ec no ogy rans er on ex v ro propaga on o

oil- producing plants that resistance to low

er ze so


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APPLICATIONBIOTECHOLOGY PRODUCTS

an pro uc v y was re uce s gn can y on cr ca

and degraded land, particularly on ex-mining land, with

ow eve o ree osp ors con en n e so ava a e

P+ for plant) and low pH (acid). One of the alternatives

o ea w suc pro em s o-reme a on on ose

lands by application of fungusMikoriza arbuskular.

Application of mikoriza intoApplication of mikoriza into J a t r o p h a J a t r o p h a seedlin increasin the ve etative rowth ofseedlin increasin the ve etative rowth ofplant, as well as increasing root capacity inplant, as well as increasing root capacity inabsorbing phosphor, nitrogen andabsorbing phosphor, nitrogen andmicronutrientsmicronutrientssuch as: Zn, Mo, Fe, Cu.such as: Zn, Mo, Fe, Cu.

Reduce the application ofReduce the application ofc em ca er zer anc em ca er zer anIncreasingIncreasing

to drought.to drought.


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Application on Corn (Zea mays) in NTT

Results on Technofert Application

No Plantsncreas ng n

Height DiameterDry

WeightSeed/Fruit

WeightNo Polong

1 Acacia 70%

2 Cacao 203%

3 Cane 43.30% 20.65%

-

5 Oil Palm 70.56%

6 Sengon 251%

7 Soybean 359% 327%

8 Sungkai 37.30% 77.80% 35.90%

10 Teak 25.88%


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Application on Critical Land

Before,

app ca on

erapplication

Ex vitro propagation

planting in the field


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Flowers and fruits in seedling, conservedFlowers and fruits in seedling, conserved

the characteristic of mother plantsthe characteristic of mother plants

Biotech Center BPPT:Biotech Center BPPT:

Training andTraining andtechnolotechnolo

transfer fortransfer for

propagationpropagation,,

as well asas well as

biobio--fertilizerfertilizera licationa lication


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J a t r o p h a Plantation

PabuaranPabuaran, Cirebon,, Cirebon,West Java, 15 HaWest Java, 15 Ha

Bandung,Bandung,es ava,es ava,

3 Ha3 Ha

Nusa Penida Island,Bali, 5 Ha


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Pantar Island, Alor,, a

Kab. Berau,


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