taming cancer by inducing immunity via e. coli presenter: xu nanfang cai danfeng chen yufei
DESCRIPTION
Protect E.coli from Immune System K1:O16 capsule Idea from “bactoblood”, UCBerkeley, iGEM2007TRANSCRIPT
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Taming Cancer by Inducing Immunity via E. coli
Presenter: Xu NanfangCai DanfengChen Yufei
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First, we need a E.coli that can escape the immune system after infusing into the blood
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Protect E.coli fromImmune SystemK1:O16 capsule
Idea from “bactoblood”, UCBerkeley, iGEM2007
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polysialic acid is a frequent coating on mammalian cells, the human immune system does not recognize K1 as foreign
Together, these surface modifications allow the bacterium to avoid detection by the immune system and should extend the serum half-life to several hours rather than the less-than-5 minutes observed with rough strains
Both of these gene clusters have been installed into the genome of MC1061 in the course of preparing our chassis strain, MC828U
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Amazing O2
First role of O2: to locate the cancer cells
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Study shows that where there’s cancer existing, oxygen level is usually significantly lower than other parts of the body.
E. coli is anaerobic
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Second role of O2: to act as a signal for the transcription of a series of factors.
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a
A
Density-sensing
Oxygen-sensing
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A
K1:O16
hydrolysis
inv
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Petra Dersch and Ralph R.Isberg: A region of the Yersinia pseudotuberculosis invasinprotein enhances integrin-mediated uptake intomammalian cells and promotes self-association
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Suicidal Component
Make sure that E.coli kills oneself after several generations of division
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Advantages:
1. Dependent on the immune system of oneself
2. Detect cancer at an early stage
3. Cancer-specific
Problems:
1. The efficiency of eliminating capsules
2. In vivo vs. in vitro