targe elector braf mutation test kit€¦ · targe elector ™ braf mutation test kit for cta an...

Target Selector ™ BRAF Mutaon Test Kit for ctDNA and ssue www.biocept.com A highly sensive test kit for accurate results BRAF V600E Tesng This Research Use Only tesng kit detects BRAF V600 mutaons in DNA derived from blood plasma or FFPE ssue secons to give insight into cancer characteriscs and provide Biomarker status of tumors such as melanoma. Benefits of Biocept Target Selector ™ Switch-Blocker Technology • Enrichment of cancer biomarker mutaon BRAF V600E in a high wild-type background • Suppression of wild-type DNA amplificaon • Proofreading DNA Polymerase resulng in lower false posives • Avoids damaged DNA templates (such as deaminated cytosines) • Ultra-high sensivity and specificity • Combinaon with Sanger sequencing for orthogonal verificaon of the enriched mutaon • Detecon of V600E as well as Wild-Type are quantave Performance with ctDNA • Stability: 1 year • Sensivity: 0.05% mutant allele fracon -single copy detecon of mutant (~3pg DNA) in up to 14000 copies of wild-type DNA background • Specificity: >99% • Effecve range: 10pg to 50ng DNA FFPE DNA preparaon and performance • Eliminates need for macrodissecon of FFPE ssue by enriching mutaons and blocking wild-type DNA amplificaon • Sensivity thresholds have been established to account for arfacts introduced by formaldehyde fixaon • Mutant allele frequency below this cut-off is considered negave for the mutaon • BRAF V600E cutoff: 0.46% These thresholds are provided as a guideline but every research facility/laboratory should establish their own thresholds based on their own criteria. • Specificity: >99% • Effecve range: 10pg to 50ng DNA F 3´ 5´ Q TACAGTGAAATCT Switch V600 WT 3´ ------CAC -------- 5´ V600E 3´ ------CTC --------5´ V600K 3´ ------CTT--------- 5´ V600R 3´ ------CCT -------- 5´ T599 WT 3´- TGT-------------5´ K601 WT 3´ ----------- TTT --- 5´ S602 WT 3´ -------------- AGA5´ BRAF Switch blocker schemac showing switch sequence and potenal mutaons in template DNA spanning switch region

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Page 1: Targe elector BRAF Mutation Test Kit€¦ · Targe elector ™ BRAF Mutation Test Kit for ctA an ssue A highly sensitive test kit for accurate results BRAF V600E Testing This Research

Target Selector™ BRAF Mutation Test Kitfor ctDNA and tissue

www.biocept.com

A highly sensitive test kit for accurate results

BRAF V600E TestingThis Research Use Only testing kit detects BRAF V600 mutations in DNA derived from blood plasma or FFPE tissue sections to give insight into cancer characteristics and provide Biomarker status of tumors such as melanoma.

Benefits of Biocept Target Selector™ Switch-Blocker Technology• Enrichment of cancer biomarker mutation BRAF V600E

in a high wild-type background

• Suppression of wild-type DNA amplification

• Proofreading DNA Polymerase resulting in lower false positives

• Avoids damaged DNA templates (such as deaminated cytosines)

• Ultra-high sensitivity and specificity

• Combination with Sanger sequencing for orthogonal verification of the enriched mutation

• Detection of V600E as well as Wild-Type are quantitative

Performance with ctDNA• Stability: 1 year

• Sensitivity: 0.05% mutant allele fraction -single copy detection of mutant (~3pg DNA) in up to 14000 copies of wild-type DNA background

• Specificity: >99%

• Effective range: 10pg to 50ng DNA

FFPE DNA preparation and performance• Eliminates need for macrodissection of FFPE tissue

by enriching mutations and blocking wild-type DNA amplification

• Sensitivity thresholds have been established to account for artifacts introduced by formaldehyde fixation

• Mutant allele frequency below this cut-off is considered negative for the mutation

• BRAF V600E cutoff: 0.46%

These thresholds are provided as a guideline but every research facility/laboratory should establish their own thresholds based on their own criteria.

• Specificity: >99%

• Effective range: 10pg to 50ng DNA

F3´5´

TACAGTGAAATCTSwitchV600 WT 3´ ------CAC --------5´V600E 3´ ------CTC --------5´V600K 3´ ------CTT---------5´V600R 3´ ------CCT --------5´T599 WT 3´- TGT-------------5´K601 WT 3´ ----------- TTT ---5´S602 WT 3´ -------------- AGA 5´

BRAF Switch blocker schematic showing switch sequence and potential mutations in template DNA spanning switch region

Page 2: Targe elector BRAF Mutation Test Kit€¦ · Targe elector ™ BRAF Mutation Test Kit for ctA an ssue A highly sensitive test kit for accurate results BRAF V600E Testing This Research

Customer Service 888-332-7729 • FAX 877-300-1761Biocept, Inc.5810 Nancy Ridge Drive, San Diego, CA 92121

www.biocept.com

Target Selector™ BRAF Mutation Test Kitfor ctDNA and tissue

Target Selector™ BRAF Mutation Test Kit—Part Number: PN 2384 This Research Use Only testing contains reagents to test 24 research samples for each target selector assay. It includes 12 reactions for standards, 1 NTC and 24 test samples for a total of 37 reactions.

Workflow

To learn more and order, please visit https://biocept.com/kits/braf/

Reagent Part number Volume total (µl) Storage (°C)

BRAF Oligo Mix 2275 135 -20

Wild Type Oligo Mix 2274 135 -20

Premix 2278 275 -20

Selector DNA Polymerase 2290 75 -20

BRAF Sequencing Primer 2405 30 -20

BRAF Standard A 2395 150 -20

Wild Type Standard A 2394 150 -20

Standard A Dilution Buffer 2300 1200 -20

DNA Suspension Buffer 2390 1200 -20