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TECHNICAL INFORMATION SCIENTIFIC LINE
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SCIENTIFIC SCIENTIFIC
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Imager CELLAVISTA Scientific NYONE Scientific
Version Highend Highend
Brightfield(LED50.000hourlifetime) ✓ ✓
Illumination 4fluorescencechannels - ✓
6fluorescencechannels ✓ -
2x(NA0.08,Resolution~6.5µmppx) Opt. Opt.
4x(NA0.2,Resolution~3.25µmppx) Opt. ✓
Resolution 10x(NA0.5,Resolution~1.3µmppx) ✓ ✓
20x(NA0.75,Resolution~0.65µmppx) ✓ ✓
40x(NA0.75,Resolution~0.35µmppx) Opt. Opt.
FLChannelUpgradepossible ✓ -
AlternativelowNAobjectivelenses
10x(NA0.3,Resolution~1.3µmppx)
20x(NA0.5,Resolution~0.65µmppx)
extensiveNikonlensselection(highNAlensestwotimesmoresensitive)
Method of Digitalimagerecognition
measurement
Microwellplates
Culture system (SBSformats6,12,24,48,96and384),
MicroscopeslidesandCulturedishes
Type sCMOS(Scientific)
Pixeldensity 2048x2048
4.19megapixel
Pixelsize 6.5x6.5µm
Fullwellcapacity 30000(1x1) 45000(1x1)
Camera Readnoise 1.8mede-/2.1rmse- 2.1mede-/2.3rmse-
Darkcurrent <0.8e-/pixel/s@10°C 15e-/pixel/s@21°C
QuantumEfficiency >81% ~80%
Digitaloutput 16bit/8bit
Refreshrate 40fps
Peltiercooled Yes No
Measurement 96-well,fullwellscan, 2minutes 3minutes
time brightfield,4xobjective
384-well,fullwellscan, 3minutes 4minutes
brightfield,4xobjective
Operating temperature 20°C-28°C(68°F-84.4°F)
Dimensions 407/625/530 350/310/620
(height/width/depth) [mm] [mm]
Weight 61kg(134lbs) 35kg(77lbs)
Energy requirements 100-240VAC,50-60Hz,295Wmaximum
CELLAVISTA® & NYONE® SCIENTIFICTechnical Specifications
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Technical Specifications
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CELLAVISTA® & NYONE® SCIENTIFICImaging Capabilities
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CELLAVISTA Scientific NYONE Scientific
Whole well imaging Yes Yes
Illumination/ Fluorescence Whitelightand6fluorescence, Whitelightand4fluorescence
excitationsources,upto6 excitationsources,upto6
fluorescenceemissionchannels fluorescenceemissionchannels
Bitdepth 8bit/16bit 8bit/16bit
External Barcode Reader Option Option
API (Plate Stacker) Yes Yes
Batch Processing Option Option
Autofocus System 1000fps 1000fps
Illumination System Electronicallyswitched Electronicallyswitched
Harmonic Motion Yes,ultrafastimaging Yes,ultrafastimaging
•Ultrafastmultipleximaging •Fastmultipleximaging
•Redesignedhighlysensitive •Highlysensitivefluorescenceoptics fluorescenceoptics
•HCS-gradelenses •HCS-gradelenses
•3timesmoresensitive:shorter •3timesmoresensitive:shorter exposuretimes,fastermeasurements exposuretimes,fastermeasurements (highthroughput),lessbleaching (highthroughput),lessbleaching
Special Features •Autofocusperformancetwice •HighDynamicRange asfastasCELLAVISTARS (21.400:1/87dB)
•HighestDynamicRange (37.500:1/91.5dB)
•Laserautofocussystem •Laserautofocussystem
•Imageanalysisduringmeasurement •Imageanalysisduringmeasurement
•Combinationofbrightfieldand •Combinationofbrightfieldand fluorescenceanalysis fluorescenceanalysis
•Automationfriendlydesign •Smallfootprint
Imaging Capabilities
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SYNENTECHigh Throughput Systems
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Automation Server Batch Processing Server Batch Processing Client
OptionalhighperformancePC
APItocontroltheimagers Highperformanceimage Controlmoduleofbatch
General purpose viathirdpartyautomation processingandexporting processingserver
platform increasingthroughput
ofautomation
Interface (Protocol) IP-Address/Port IP-Address/Port IP-Address/Port
Connection GigE GigE GigE
•Fullexternalcontrol •Parallelprocessing •Detailedcontrolof
•Measurements ofmeasurements Batchprocessingserver
•Imageprocessing •LiveFolder •Reprocess
•Exporting •Automationclient •Export
Features •Reprocessingofold •Processandexport
experiments •Generalsetup
•UpdatingIP-settings
•Processingof
thirdpartyimages
Automation and batch processing features
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SYNENTECAutomation and Batch Processing System
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==
= ==
=
= =
=
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Datastoragebuffer
Datastorageandimageanalysis
InstalledhereAutomation
schedulingPC
Batchprocessingsoftwarepackage+optionalhighperformancebatch
processingserver(BPS)
Installedhere
AutomationInterfaceNYONE / CELLAVISTA
ControllingPC
OptionalNASGigE connectionswithstaticIPsviaGigE-switch
BatchClient(Software)
BatchServer(Software)
=BPS
NAS
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SYNENTECImaging Capabilities
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YT-Softwareenablesswitchingfrom8bitto16bit
HigherdynamicrangeandamagnitudemoresensitivethanCLDline
16 Bit Imaging Advantages
Thehistogramsabove,imagedwithNYONEScientificusingFACScalibrationbeadsdemonstratethatNYONEScientificandCELLAVISTAScientificareexcellenttoolstocreateoutstandingresultsintermsofquantification,robustnessandquality.
CLD
SCIENTIFIC
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10x 0.4 NA20x, 0.5 NA
10x 0.4 NA20x, 0.5 NA
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SYNENTECCapabilities of CELLAVISTA and NYONE in cell based assays
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ANTIBODYBINDING APOPTOSIS SPHEROIDIMAGING
ICC/IHC FOCUSFORMINGASSAY ANTIBODYINTERNALIZATION
[email protected]μm/px [email protected]μm/px [email protected]μm/px
CLONEGALLERY
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• CRISPR/Cas Gene Editing
• Single Cell Cloning (SCC/ FASCC)
• Trypan Blue Viability (Trypan Blue-Kit®)
• FASC Seeding Control
• Transfection Efficiency
• mAb-Aggregate Screening (mAbregation-Kit®)
• IgG (Fc/Fab) Quantitation (PAIA-Assay®)
• Confluence
• FISH Imaging
• iPS-Cell Detection
• CD-Antigen detection
• Apoptosis Monitoring
• Toxicity Studies
• Nuclei Count/ Organelle Characterisation
• ICC / IHC (Multiplex Imaging)
• Total Well Intensity
• Wound Healing
• Antibody Internalization
• Focus Forming Assay
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D-25337 Elmshorn
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