technical program -...
TRANSCRIPT
Technical Program
Tuesday, December 2 15:00–15:15 Opening Plenary Lectures–1 (Funai Auditorium) (Chair: Koji Otsuka, Edward S. Yeung) 15:15–16:00 L1 SEPARATIONS ON THE BRAIN: NEW INSTRUMENTATION AND
APPLICATIONS OF MICROSCALE SEPARATIONS TO NEUROSCIENCE Robert T. Kennedy *, Qiang Li, Greg T. Roman, Maura Perry, Meng Wang Department of Chemistry, University of Michigan, 930 N. University Ave, Ann Arbor, MI,
48109-1055, USA 16:00–16:15 Break 16:15–16:50 L2 ENGINEERING PLATFORM OF MICRO AND EXTENDED-NANO FLUIDICS
FOR HIGH PERFORMANCE ANALYTICAL DEVICES Takehiko Kitamori *(1, 2) (1) Department of Applied Chemistry, Graduate School of Engineering, The University of
Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan; (2) Micro Chemistry Group, Kanagawa Academy of Science and Technology, KSP BLDG, East 307, 3-2-1 Sakado, Takatsu-ku, Kawasaki, Kanagawa 213-0012, Japan
16:50–17:25 L3 ON THE RELATION BETWEEN COLUMN STRUCTURE AND
CHROMATOGRAPHIC PERFORMANCE Gert Desmet *, J. Billen, D. Cabooter, F. Detobel, W. De Malsche, H. Eghbali, S. De
Bruyne, J. Vangelooven Vrije Universiteit Brussel, Department of Chemical Engineering (CHIS-IR), Pleinlaan 2,
1050 Brussels, Belgium 18:00–19:30 Welcome Reception sponsored by Shimadzu Corp (Café Arte).
Wednesday, December 3 Oral Session 1 High Performance Separations (Funai Auditorium) (Chair: Paul Haddad, Yukui Zhang) 9:15–9:40 L4 COMPARISON OF SILICA AND POLYMERIC PARTICLES FOR
PHARMACEUTICAL ANALYSIS Pat Sandra * Research Institute for Chromatography, Kennedypark 26, B-8500 Kortrijk, Belgium 9:40–10:00 L5 HIGH EFFICIENCY HPLC SEPARATIONS OF PROTEINS AND PEPTIDES IN
CAPILLARIES AND MICROCHIPS USING POROUS POLYMER MONOLITHS AS STATIONARY PHASES
Frantisek Svec * (1, 2), Jana Krenkova (1), Pavel Levkin (2), Ivo Nischang (2), Yan Xu (2) (1) The Molecular Foundry, E.O. Lawrence Berkeley National Laboratory, Berkeley, CA 94720-8197, USA; (2) Department of Chemistry, University of California, Berkeley, CA 94720-1460, USA
10:00–10:20 L6 UPLC® THEORY AND PRACTICE Uwe D. Neue *, Thomas H. Walter, Pamela C. Iraneta, Diane M. Diehl
Waters Corporation, 34 Maple St., Milford, MA 01757, USA 10:20–10:40 L7 MOLECULARLY IMPRINTED POLYMERS AS AFFINITY MEDIA
Jun Haginaka * Faculty of Pharmaceutical Sciences, Mukogawa Women’s University, 11-68, Koshien Kyuban-cho, Nishinomiya 663-8179, Japan
10:40–11:00 Break (Oral Sessions 2 and 3, Sessions 4 and 5, and Sessions 6 and 7 are parallel sessions.) Oral Session 2 LC Columns and Packings (Funai Auditorium) (Chair: Jun Haginaka, Frantisek Svec) 11:00–11:25 L8 PROBLEMS IN THE ANALYSIS OF PHARMACEUTICALS AND OTHER BASIC
SOLUTES IN HPLC: SOME SOLUTIONS USING RECENTLY DEVELOPED COLUMNS
David V. McCalley * School of Life Sciences, University of the West of England, Frenchay, Bristol BS16 1QY,
U.K. 11:25–11:45 L9 ADVANCES IN ION CHROMATOGRAPHY: SMARTER, FASTER, MORE
POWERFUL Paul R. Haddad * Australian Centre for Research on Separation Science, School of Chemistry, University of
Tasmania, Private Bag 75 Hobart, 7001, Tasmania, Australia 11:45–12:05 L10 GENERATION OF PLUG-LIKE CHROMATOGRAPHIC BANDS IN
PREPARATIVE LIQUID CHROMATOGRAPHIC COLUMN BY USING CONICAL SHAPE WALL COLUMN
Liejuan Lu, Jie Chen, Yafeng Guan * Department of Instrumentation & Analytical Chemistry,Dalian Institute of Chemical
Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China 12:05–12:25 L11 NEW MOMENT EQUATIONS FOR ANALYSIS OF CHROMATOGRAPHIC
BEHAVIOR Kanji Miyabe * Graduate School of Science and Engineering for Research, University of Toyama, 3190,
Gofuku, Toyama 930-8555, Japan Oral Session 3 CE Separations (Katsura Hall) (Chair: Kiyohito Shimura, Yi Chen) 11:00–11:25 L12 CAPILLARY ELECTROPHORETIC SEPARATION OF CARBON
NANOPARTICLES Luis A. Colon *, Jared S. Baker, Glorimar Vicente Department of Chemistry, University at Buffalo, The State University of New York, Natural
Science Complex Buffalo, NY 14260-3000, USA 11:25–11:45 L13 DETERMINATION OF AMINES AND AMINO ACIDS BY CAPILLARY
ELECTROPHORESIS WITH FLUORESCENCE DETECTION Chuan-Kuo Chen (1), Tai-Chia Chiu (2), Huan-Tsung Chang *(1) (1) Department of Chemistry, National Taiwan University, Taipei 106, Taiwan; (2) Department of Applied Science, National Taitung University, Taitung 890, Taiwan 11:45–12:05 L14 METABONOMIC STUDIES OF NUCLEOSIDES PROFILES IN UROGENITAL
CANCER DISEASES Michał J. Markuszewski *, Ewa Szymańska, Małgorzata Waszczuk-Jankowska, Roman
Kaliszan Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdańsk,
Gen. J. Hallera 107, 80-416 Gdańsk, Poland 12:05–12:25 L15 REAGENT RELEASE CAPILLARY-BASED CAPILLARY-ASSEMBLED
MICROCHIP (RRC-BASED CAs-CHIP): TOWARDS THE DEVELOPMENT OF VERSATILE DISPOSABLE MICRO DEVICES FOR CHEMICAL SENSING AND ELECTROPHORETIC SEPARATION
Hideaki Hisamoto *, Terence G. Henares, Masaki Kataoka, Hiroki Yokoyama, Toshio Yao
Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuencho, Naka, Sakai, Osaka 599-8531, Japan
12:30–14:30 Lunch (Lunch on own) Luncheon Seminar (12:30–13:30) Shimadzu Corporation (Meeting Room 22AB of Funai Auditorium) TBA GL Sciences Inc. (Meeting Room 34 of Funai Auditorium) TBA Poster Session 1 Oral Session 4 Multi-Dimensional Separations (Funai Auditorium) (Chair: David McCalley, Kanji Miyabe) 14:30–14:50 L16 OPTIMIZATION OF OFF-LINE COMPREHENSIVE TWO-DIMENSIONAL
HPLC Georges Guiochon *, Krisztìan Horvàth, Jacob Fairchild Department of Chemistry, The University of Tennessee, Knoxville, TN, 37996-1600, USA;
Division of Chemical Sciences, Oak Ridge National Laboratory, Oak Ridge, TN, USA 14:50–15:10 L17 STRUCTURED SEPARATIONS OF COMPLEX SAMPLES BY
TWO-DIMENSIONAL CHROMATOGRAPHY Peter Schoenmakers * Polymer-Analysis Group, Faculty of Science, University of Amsterdam, Nieuwe
Achtergracht 166, 1018 WV Amsterdam, The Netherlands 15:10–15:30 L18 COMPREHENSIVE LC COUPLED TO IT-TOF MASS SPECTROMETRY FOR
EXACT MOLECULES CONFIRMATION Luigi Mondello *
Dipartimento Farmaco-chimico, Facoltà di Farmacia, Università di Messina, viale Annunziata, 98168 – Messina, Italy and Campus Biomedico, Via Alvaro del Portillo 21, 00128 - Roma, Italy
15:30–15:50 L19 HIGH RESOLUTION DIASTEREOMER SEPARATIONS OF OLIGOSTYRENES
USING TWO-DIMENSIONAL HPLC R. Andrew Shalliker *
Australian Centre for Research On Separation Science (ACROSS) University of Western Sydney Node, and Nanoscale Organisation and Dynamics Group, University of Western Sydney, Locked Bag 1797, South Penrith DC, South Penrith, 1797 NSW, Australia
Oral Session 5 LC Applications: Proteins and Peptides (Katsura Hall) (Chair: Frantisek Foret, Jin-Ming Lin) 14:30–14:50 L20 INTACT PROTEIN BASED MULTIDIMENSIONAL CHROMATOGRAPHIC
SEPARATION AND MASS SPECTROMETRIC IDENTIFICATION FOR LIVER PROTEOME ANALYSIS
Xiangmin Zhang *(1, 2), Mingxia Gao (1), Xia Guan (1), Guangfeng Hong (1), Guoquan
Yan (1), Chunhui Deng (1) (1) Department of Chemistry, Fudan University, Shanghai 200433, China;(2) Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China
14:50–15:10 L21 POSTCOLUMN DERIVATIZATION OF PROTEINS IN CAPILLARY SIEVING
ELECTROPHORESIS Takashi Kaneta *(1, 2), Daisuke Yamamoto (1), Totaro Imasaka (1, 2) (1) Department of Applied Chemistry, Graduate School of Engineering, Kyushu University,744, Motooka, Nishi-ku, Fukuoka 819-0395, Japan; (2) Division of
Translational Research, Center for Future Chemistry, Kyushu University, 744, Motooka, Nishi-ku, Fukuoka 819-0395, Japan
15:10–15:30 L22 A NEW STRATEGY FOR SEPARATION OF INTACT PROTEINS IN
PREPARATIVE SCALE BY LIQUID CHROMATOGRAPHY Xindu Geng *
Institute of Modern Separation Science, Northwest University; Shaanxi Key Laboratory of Modern Separation Science, Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Xi’an 710069 China
15:30–15:50 L23 MONOLITHIC MATERIALS AND THEIR APPLICATION IN PROTEIN
ANALYSIS Lihua Zhang *, Zhen Liang, Yukui Zhang
National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
15:50–16:10 Break Oral Session 6 CE–MS, LC/MS (Funai Auditorium) (Chair: Luigi Mondello, Xiangmin Zhang) 16:10–16:30 L24 NOVEL SEPARATION AND IDENTIFICATION TECHNIQUES FOR
PROTEOME STUDY Yukui Zhang *
National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
16:30–16:50 L25 TECHNIQUES FOR DIRECT INJECTION OF BIOLOGICAL FLUIDS IN
CAPILLARY SEPARATION SYSTEMS Douglas Westerlund *(1), Ingela Sundström (1), Per Andrén (2), Reda Jarmalavičiené (3), Audrius Marušhka (3)
(1) Analytical Pharmaceutical Chemistry, Uppsala University, Sweden; (2) GE Health, Uppsala, Sweden; (3) Dept. of Chemistry, Vytautas Magnus University, Kaunas, Lithuania
16:50–17:10 L26 COMBINATION OF ELECTRIC FIELD AND MEMBRANE FACILITATES
CONTINUOUS ADSORPTION FOR SAMPLE PREPARATION OF PROTEINS FOR DIRECT ESI-MS ANALYSIS
Huwei Liu *(1), Yu Zhou (1), Hailin Shen (2), Tie Yi (3), Nannan Pang (1), Jie Liao (4) (1) Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China; (2) Manchester Interdisciplinary Biocentre, the University of Manchester, Manchester M1 7DN, United Kingdom; (3) College of Information Science and Engineering, Central South University, Changsha 410083, China; (4) Medical Experiment & Analysis Center, General Hospital of Chinese PLA, Beijing 100853, China
17:10–17:25 L27 DEVELOPMENT OF PHOSPHOPEPTIDE-FOCUSED NANOLC-MS SYSTEMS
FOR HIGH PERFORMANCE PHOSPHOPROTEOMICS Yutaka Kyono *(1, 2), Naoyuki Sugiyama (1, 3), Masaru Tomita (1, 3), Yasushi Ishihama
(1, 4)
(1) Institute for Advanced Biosciences, Keio University, Daihoji, Tsuruoka, Yamagata 997-0017, Japan; (2) GL Sciences Inc., Nishishinjuku, Shinjuku-ku, Tokyo 163-1130, Japan; (3) Human Metabolome Technologies Inc., Kakuganji, Tsuruoka, Yamagata 997-0052, Japan; (4) PRESTO, Japan Science and Technology Agency, Tokyo, Japan
17:25–17:40 L28 A NOVEL HUMAN PANCREATIC CANCER BIOMARKER DISCOVERY USING
AFFINITY CHROMATOGRAPHY WITH MALDI-TOF MS Quan Bai *
Institute of Modern Separation Science, Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Northwest University, Xi’an 710069, China
Oral Session 7 Environmental Applications (Katsura Hall) (Chair: Tetsuo Okada, Janusz Pawliszyn) 16:10–16:30 L29 SEPARATION AND IDENTIFICATION OF A NOVEL HETEROCYCLIC
BROMINATED FLAME RETARDANT IN ENVIRONMENT BY HPLC/MS/MS Ruan Ting, WangYawei, Shi Jianbo, Jiang Guibin *
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences,P.O. Box 2871, Beijing, 100085, China
16:30–16:50 L30 COMPOUND CLASS SELECTIVE LIQUID CHROMATOGRAPHY Torsten Spitzer *
Environmental, Industrial and Food Analysis, Postfach 1428, D-38334 Helmstedt 16:50–17:10 L31 SIMULTANEOUS DETERMINATION OF TEN ESTROGENS AND THEIR
METABOLITES IN ENVIRONMENTAL WATERS USING IMPROVED TWO-STEP SOLID-PHASE EXTRACTION FOLLOWED BY LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY
Wei Yan (1), Jin-Ming Lin *(2) (1) Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing 100085, China; (2) Department of Chemistry, Tsinghua University, Beijing 100084, China
17:10–17:25 L32 DETERMINATION OF VANADIUM AS 2-(5-BROMO-2-PYRIDYLAZO)-5-
DIETHYLAMINOPHENOL (BR-PADDAP)-HYDROGEN PEROXIDE TERNARY COMPLEX IN SEA WATER, SOIL AND FERTILIZER BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Narumol Vachirapatama *(1), Danuwat Sinturong (1), Paul R. Haddad (2) (1) Department of Chemistry, Faculty of Science and Technology, Thammasat University, Rangsit, Pathumthani, 12121, Thailand; (2) ACROSS, School of Chemistry, Faculty of Science and Engineering, University of Tasmania, GPO Box 252-75, Hobart, Tasmania, 7001, Australia
17:25–17:40 L33 DETERMINATION OF CARBAMATE PESTICIDES IN VEGETABLES AND
FRUITS BY ON-LINE GPC-LCMS SYSTEM Yuki Hashi *(1, 2), Feng Ji (1), Feng-Yun Pan (1), Jin-Ming Lin (2)
(1) Shimadzu International Trading (Shanghai) Co., Limited, Beijing Analytical Applications Center, Beijing, 100020, P.R. China; (2) The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University, Beijing 100084, P.R. China
19:00–21:30 Evening Sessions 1-3 (Kyoto Royal Hotel & Spa) (Evening Sessions 1, 2, and 3 are parallel sessions.) 19:00–19:30 Meal served
Evening Session 1 Agilent Technologies A (Chair: Ron Majors) 19:30–20:00 ES1 Sponsor Presentation Ron Majors Agilent Technologies 20:05–20:20 E1 PREPARATION AND CE APPLICATIONS OF THE CAPILLARY COATED
DYNAMICALLY WITH CARBOXYMETHYL CHITOSAN Yi Liu *, Xiaofang Fu, Yu Bai, Huwei Liu
Institute of Analytical Chemistry, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
20:20–20:35 E2 POLYMER SIZE CHARACTERIZATION BY FREE SOLUTION CAPILLARY
ELECTROPHORESIS Zhenpeng Guo *, Yi Chen
Beijing National Laboratory of Molecular Science, Laboratory of Analytical Chemistry for Life Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, People’s Republic of China
20:35–20:50 E3 DETERMINATION OF AMPROLIUM IN FEEDING STUFFS, EGGS AND
CHICKEN MUSCLE BY FIELD AMPLIFIED SAMPLE INJECTION-CAPILLARY ZONE ELECTROPHORESIS
Anna Martínez-Villalba *, Óscar Núñez, Encarnación Moyano, Mª Teresa Galceran Department of Analytical Chemistry, University of Barcelona, Martí i Franquès 1-11, 08028, Barcelona, Spain
20:50–21:05 E4 DROPLET-ARRAY LIQUID CHROMATOGRAPHY ON A MICROFLUIDIC
CHIP USING DROPLET TRAPPING TECHNIQUE Ying Zhu *, Hong Chen, Qun Fang
Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou, China, 310058
21:05–21:20 E5 QUANTITATIVE PHOSPHOPROTEOME ANALYSIS USING A CALCINED
TITANIA/C18 BIPHASIC COLUMN AND A NOVEL STABLE ISOTOPE LABELING APPROACH Koshi Imami *(1), Naoyuki Sugiyama (1, 2), Yutaka Kyono (1, 3), Masaru Tomita (1, 2), Yasushi Ishihama (1, 4) (1) Institute for Advanced Biosciences, Keio University, Daihoji, Tsuruoka, 997-0017, Japan; (2) HMT Inc., Kakuganji, Tsuruoka 997-0052, Japan; (3) GL Sciences Inc., Sayamagahara, Iruma, Saitama 358-0032, Japan; (4) PRESTO, Japan Science and Technology Agency, Chiyoda, Tokyo 102-0075, Japan
21:20–21:30 Award Presentation Evening Session 2 Agilent Technologies B (Chair: Gerard Rozing) 19:30–20:00 ES2 Sponsor Presentation INFLUENCE OF NANO-HPLC-COLUMN CONDUIT SHAPE AND COLUMN
ASPECT RATIO ON CHROMATOGRAPHIC PERFORMANCE Steffen Ehlert (1), Stephanie Jung (1), Ulrich Tallarek (1), Monika Dittmann (2), Karsten
Kraiczek (2), Jose Mora (2), Lukas Trojer (2), Gerard Rozing *(2) (1) Department of Chemistry, Philipps-Universität, Marburg, Germany; (2) Agilent
Technologies, 76228 Waldbronn, Germany 20:05–20:20 E6 GLOBAL METABOLIC PROFILING STUDIES ON SEVERE
PROTEIN-ENERGY MALNUTRITION IN RAT WITH RAPID & RESOLUTION
LIQUID CHROMATOGRAPHY COUPLED TO QUADRUPOLE-TIME OF FLIGHT MASS SPECTROMETRY
Zeming Wu *, Chunxia Zhao, Peiyuan Yin, Guowang Xu National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
20:20–20:35 E7 APPLICATIONS OF MONOLITHIC COLUMN TECHNOLOGY IN
LABEL-FREE QUANTITATIVE PROTEOME ANALYSIS Fangjun Wang *, Mingliang Ye, Ren’an Wu, Hanfa Zou
Key Lab of Separation Sciences for Analytical Chemistry, National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
20:35–20:50 E8 LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY IN A HYPERBOLIC
TRIPLE QUADRUPOLE INSTRUMENT FOR THE MULTI-RESIDUE ANALYSIS OF PESTICIDES Oscar Nuñez *(1), Héctor Gallart-Ayala (1), Imma Ferrer (2), Encarnación Moyano (1), M. Teresa Galceran (1) (1) Department of Analytical Chemistry, University of Barcelona, Martí I Franquès, 1-11, 08028 Barcelona, Spain; (2) Pesticide Residue Research Group, University of Almeria, Almeria, Spain
20:50–21:05 E9 ANALYSIS OF DIESEL BY MEANS OF OFF-LINE TRIDIMENSIONAL
COMPREHENSIVE CHROMATOGRAPHY LC-GCXGC COUPLED WITH A MS DETECTOR Danilo Sciarrone *(1), Peter Q. Tranchida (1), Paola Dugo (2), Giovanni Dugo (1), Luigi Mondello (1) (1) Dipartimento Farmaco-chimico, Università di Messina, Viale Annunziata, 98168 Messina, Italia; (2) Dipartimento di Scienze degli Alimenti e dell’Ambiente, Università di Messina, Salita Sperone, 98166 Messina, Italia
21:05–21:20 E10 MULTIDIMENSIONAL SEPARATION PLATFORM BASED ON COLUMN
SWITCH RECYCLING SIZE EXCLUSION CHROMATOGRAPHY FOR HIGH EFFICIENT ANALYSIS OF PROTEINS Huiming Yuan * (1, 2), Lihua Zhang (1), Zhen Liang (1), Yukui Zhang (1) (1) National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116023, China; (2) Graduate School of Chinese Academy of Sciences, Beijing 100039, China
21:20–21:30 Award Presentation Evening Session 3 Merck (Chair: Robertus Hendriks) 19:30–19:45 ES3 Sponsor Presentation ABOUT THE INFLUENCE OF THE MACROPORE- AND DOMAIN SIZE ON
THE CHROMATOGRAPHIC PERFORMANCE OF SILICA-BASED MONOLITHS
Robertus Hendriks *, A. Piper, P. Knoell, Y. Faupel, K. Kreher, S. Altmaier, K. Cabrera Merck KGaA, Darmstadt, Germany 19:45–20:00 ES4 Sponsor Presentation BIOANALYSIS BY HILIC ON A BONDED ZWITTERIONIC STATIONARY
PHASE Einar Ponten * Merck SeQuant AB, Box 7956, S-907 19, Umea, Sweden 20:05–20:20 E11 ADSORPTION/PARTITION MODE SWITCHING IN ICE CHROMATOGRAPHY
BY UTILIZING PHASE EQUILIBRIUM IN NaCl- OF KCl-WATER SYSTEM Yuiko Tasaki *, Tetsuo Okada
Department of Chemistry, Tokyo Institute of Technology, Ookayama, Meguro-ku, Tokyo 152-8551, Japan
20:20–20:35 E12 SYNTHESIS OF POLY(4-VINYLPYRIDINE-CO-METHYL ACRYLATE)-
GRAFTED SILICA STATIONARY PHASE AS POLYMERIC ORGANIC PHASE FOR SELECTIVITY TUNING TOWARDS PAHS IN REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Abul K. Mallik *, Makoto Takafuji, Hirotaka Ihara Department of Applied Chemistry and Biochemistry, Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan
20:35–20:50 E13 IMPROVING THE HOMOGENEITY OF MONOLITHIC SILICA COLUMNS
USING 2D MICRO-PILLAR ARRAYS Frederik Detobel *(1), Hamed Eghbali (1), Selm De Bruyne (1, 2), Vincent Verdoold (2), Han Gardeniers (2), Gert Desmet (1) (1) Department of Chemical Engineering, FirW-CHIS, Vrije Universiteit Brussel, B-1050 Brussels, Belgium; (2) Research Programme Mesofluidics, MESA+ Institute for Nanotechnology, MESA+ Research Institute, 7500 AE Enschede, The Netherlands
20:50–21:05 E14 DEVELOPMENT OF RETENTION PREDICTION MODELS FOR
ADRENORECEPTOR AGONISTS AND ANTAGONISTS ON POLAR STATIONARY PHASES IN HYDROPHILIC INTERACTION CHROMATOGRAPHY Noel S. Quiming *(1, 2), Nerissa L. Denola (2, 3), Yoshihiro Saito (2), Kiyokatsu Jinno (2) (1) Department of Physical Sciences and Mathematics, College of Arts and Sciences, University of the Philippines Manila, Manila, 1000, Philippines; (2) School of Materials Science, Toyohashi University of Technology, Toyohashi, 441-8580, Japan; (3) Department of Pharmaceutical Chemistry, College of Pharmacy, University of the Philippines Manila, Manila, 1000, Philippines
21:05–21:20 E15 DEVELOPMENT OF PRESSURE-DRIVEN SEPARATION FOR SMALL
COMPOUNDS IN EXTENDED-NANO SPACES Masanori Inaba *(1), Masaru Kato (1, 2), Takehiko Tsukahara (1), Kazuma Mawatari (3), Akihide Hibara (1), Takehiko Kitamori (1, 2, 3) (1) Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan; (2) Center for NanoBio Integration, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan; (3) Kanagawa Academy of Science and Technology (KAST), Sakado, Takatsu-ku, Kawasaki Kanagawa, 213-0021, Japan
21:20–21:30 Award Presentation
Thursday, December 4 Oral Session 8 Biochemistry (Funai Auditorium) (Chair: Hian Kee Lee, Douglas Westerlund) 9:15–9:40 L34 CELLULAR MEMBRANE AFFINITY CHROMATOGRAPHY: WORKING ON
THE INTERSECTION OF CHROMATOGRAPHIC AND BIOLOGICAL SCIENCES
Tomoko Kitabatake (1, 2), Prateek Bhatia (1), Ruin Moaddel (1), Ron Cole (1), Mukesh Gandhari (1), Chester Frazier (1), Julia Hartenstein (1), Avraham Rosenberg (1), Harrison K. Musyimi (1), Charlene Bashore (1), Michel Bernier (1), Irving W. Wainer *(1) (1) Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA; (2) Current address: Faculty of Pharmaceutical Sciences, Mukogawa Women’s University, 11-68, Koshien Kyuban-cho, Nishinomiya 663-8179, Japan
9:40–10:00 L35 ISOLATION AND IDENTIFICATION OF MAJOR AND MINOR FLAVONOIDS
IN HEARTSEASE (VIOLA TRICOLOR L.) BY NANOLC-MSn AND NMR Andras Guttman *, Viktoria Vukics, Thomas Ringer
Horváth Laboratory of Bioseparation Sciences, Institute of Analytical Chemistry and Radiochemistry, University of Innsbruck, Innrain 52, Austria
10:00–10:20 L36 DETERMINATION OF NAD+ AND NADH LEVELS IN A SINGLE CELL UNDER
H2O2 STRESS BY CAPILLARY ELECTROPHORESIS Edward S. Yeung *, Wenjun Xie
Ames Laboratory-USDOE and Department of Chemistry, Iowa State University, Ames, IA 50011, USA
10:20–10:40 L37 CHALLENGES IN THE DEVELOPMENT OF THERAPEUTIC VACCINES John Frenz *
GlobeImmune, Inc., 1450 Infinite Drive, Louisville, CO 80027, USA 10:40–11:00 Break (Oral Sessions 9 and 10, Sessions 11 and 12, and Sessions 13 and 14 are parallel sessions.) Oral Session 9 Sample Preparation (Funai Auditorium) (Chair: Jiang Guibin, Andras Guttman) 11:00–11:25 L38 AUTOMATION OF SPME-LC-MS/MS USING MULTI-WELL PLATES AND
APPLICATIONS T HIGH THROUGHPUT BIOANALYSIS AND DRUG-PROTEIN BINDING STUDIES
Janusz Pawliszyn *, Dajana Vuckovic, Erasmus Cudjoe Department of Chemistry, University of Waterloo, 200 University Avenue West, Waterloo,
Canada 11:25–11:45 L39 EVALUATING POLYMER MONOLITH MICROEXTRACTION COUPLED TO
HPLC/QTOF MASS SPECTROMETRY FOR QUANTIFICATION AND CONFIRMATION OF QUINOLONE RESIDUES IN FOOD
Ming-Ming Zheng, Ge-Deng Ruan, Yu-Qi Feng * Department of Chemistry, Wuhan University, Wuhan 430072, China 11:45–12:05 L40 WATER, A CRITICAL RESOURCE: SAMPLE PREPARATION BY
ENVIRONMENTALLY FRIENDLY MICROEXTRACTION PROCEDURES Hian Kee Lee * Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore
117543 12:05–12:25 L41 USE OF IONIC LIQUIDS AS MEMBRANE LIQUIDS FOR LIQUID-PHASE
MICROEXTRACTION AND AS ION PAIR REAGENTS FOR HPLC SEPARATION OF SOME POLAR ORGANIC CONTAMINANTS
Jing-fu Liu *, Yong Tao, Yuang-jian Huan, Gui-bin Jiang State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center
for Eco-Environmental Sciences, Chinese Academy of Sciences, P. O. Box 2871, Beijing 100085, China
Oral Session 10 Separations for Life Sciences (Katsura Hall) (Chair: Jacques Crommen, Kenji Hamase) 11:00–11:25 L42 ANALYSIS OF WATER-EXTRACTABLE OF NON-STRUCTURAL
CARBOHYDRATES (NSC), INCLUDING INULIN AND STARCH, IN DIFFERENT PLANT SAMPLES BY HPAEC-PAD
Michael Raessler * Max-Planck-Institut fuer Biogeochemie, SpecLab, Hans-Knoell-Str. 10, D-07745 Jena,
Germany 11:25–11:45 L43 HPLC DETERMINATION OF PYRIDINIUM ALDOXIMES IN BODY FLUIDS Huba Kalasz *(1), Kornelia Tekes (2), Georg Petroianu (3)
(1) Department of Pharmacology, Semmelweis University, Budapest, Hungary; (2) Department of Pharmacodynamics, Semmelweis University, Budapest, Hungary; (3) Department of Pharmacology and Therapeutics, United Arab Emirates University, Al Ain, United Arab Emirates
11:45–12:05 L44 DESIGN AND SCREENING OF PEPTIDE PROBE FOR TARGETING CANCER
CELLS BY HIGH PERFORMANCE AFFINITY CHROMATOGRAPHY Rui Zhao *(1), Yanyan Huang (1), Rouli Zhou (2), Yabin Fu (2), Guoquan Liu (1), Yi
Chen (1) (1) Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China; (2) School of Basic Medical Sciences, Peking University, Beijing, 100191, China
12:05–12:25 L45 SEPARATION OF NANOFIBERS (CARBON NANOTUBE AND β-AMYLOID)
USING CE Masaru Kato *, Tatsuhiro Yamamoto Center for NanoBio Integration, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo
113-8656, Japan 12:30–14:30 Lunch (Lunch on own) Luncheon Seminar (12:30–13:30) Agilent Technologies (Meeting Room 22AB of Funai Auditorium) PEPTIDE QUANTITATION WITH AGILENT HPLC-CHIP/TRIPLE QUAD MS UNTARGETED METABOLOMICS ANALYSIS USING LC-(Q)TOF Merck Ltd., Japan (Meeting Room 34 of Funai Auditorium) TBA Poster Session 2 Oral Session 11 Proteomics (Funai Auditorium) (Chair: Bifeng Liu, R. Andrew Shalliker) 14:30–14:50 L46 THE USE OF PLASMA PROTEOMICS TO STUDY DISEASE PATHOGENESIS William S. Hancock *(1), Marina Hincapie (1), Majlinda Kullolli (1), Tatiana Plavina (2) (1) Barnett Institute and Department of Chemistry and Chemical Biology, Northeastern
University,Boston, MA 02115, USA; (2) Biogen Idec, Inc., Boston, MA 02142, USA 14:50–15:10 L47 SAMPLE PREPARATION AND DATA PROCESSES FOR
PHOSPHOPROTEOME ANALYSIS Hanfa Zou *, Mingliang Ye, Xinning Jiang, Guanghui Han, Fangjun Wang National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China 15:10–15:30 L48 MICRO AND MEZZO SCALE ANALYTICAL SYSTEMS FOR MASS
SPECTROMETRY Frantisek Foret *, Jakub Grym, Roman Tomas, Karel Kleparnik
Institute of Analytical Chemistry of the ASCR, v.v.i., Veveri 97, 602 00 Brno, Czech Republic
15:30–15:50 L49 MACROMOLECULAR SEPARATION AND ANALYSIS IN BIOMARKER
DISCOVERY Jonas Bergquist *
Analytical Chemistry & Neurochemistry, Department of Physical and Analytical Chemistry, Biomedical Centre, Uppsala University, PO Box 599, SE-751 24 Uppsala, Sweden
Oral Session 12 Chiral Separations (Katsura Hall) (Chair: Daniel W. Armstrong, Nariyasu Mano) 14:30–14:50 L50 INFLUENCE OF THE BACKGROUND ELECTROLYTE COMPOSITION AND
ELECTROSPRAY PARAMETERS ON THE ANALYTE RESPONSE IN CYCLODEXTRIN MEDIATED NONAQUEOUS CAPILLARY ELECTROPHORESIS-MASS SPECTROMETRY
Anne-Catherine Servais (1), Marianne Fillet (1), Anne Rousseau (1), Govert W. Somsen (2), Gerhardus J. De Jong (2), Jacques Crommen *(1) (1) Laboratory of Analytical Pharmaceutical Chemistry, Bioanalytical Chemistry Research Unit, Dept. of Pharmaceutical Sciences, University of Liege, CHU, B36, B-4000 Liege, Belgium; (2) Dept. of Biomedical Analysis, Utrecht University, P.O. Box 80082, NL-3508 TB Utrecht, The Netherlands
14:50–15:10 L51 ENANTIOSEPARATION BY CAPILLARY ELECTROPHORESIS Yi Chen * Beijing National Lab of Molecular Science, and Lab of Analytical Chemistry for Life
Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China 15:10–15:30 L52 NEW CHIRAL STATIONARY PHASES FOR LIQUID-PHASE
ENANTIOSEPARATION TECHNIQUES Bezhan Chankvetadze *
Department of Physical and Analytical Chemistry and Molecular Recognition and Separation Science Laboratory, School of Exact and Natural Sciences, Tbilisi State University, Tbilisi 0128, Georgia
15:30–15:50 L53 DEVELOPMENT OF THE HPLC CHIRAL STATIONARY PHASES BASED ON
CHIRAL CROWN ETHERS Myung Ho Hyun *
Department of Chemistry, Pusan national University, Geumjeong-gu, Busan 609-735, Korea
15:50–16:10 Break Oral Session 13 LC Columns and Packings (Funai Auditorium) (Chair: Gert Desmet, Yafeng Guan) 16:10–16:30 L54 USING VERY FINE PARTICLES IN LIQUID CHROMATOGRAPHY:
CONSEQUENCES ON RETENTION AND BAND BROADENING Fabrice Gritti *, Georges Guiochon
Department of Chemistry, The University of Tennessee, Knoxville, TN, 37996-1600, USA and Division of Chemical Sciences, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA
16:30–16:50 L55 THE PHYSICAL CHARACTERISATION OF MONOLITHIC (AND PACKED)
CAPILLARY COLUMNS, COVALENTLY MODIFIED WITH IONIC SPECIES, USING ON-CAPILLARY CONTACTLESS CONDUCTIVITY DETECTION
Brett Paull *(1, 2), Damian Connolly (1), Eoin Gillespie (1), Miroslav Macka (2), Pavel N. Nesterenko (3) (1) Centre for Bioanalytical Science, School of Chemical Sciences, Dublin City University,
Glasnevin, Dublin 9, Ireland; (2) National Centre for Sensor Research, School of Chemical Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland; (3) Australian Centre for Research on Separation Science, University of Tasmania, Hobart, 7001,
Tasmania, Australia 16:50–17:10 L56 PREPARATION AND EVALUATION OF MONOLITHIC SILICA COLUMNS
FOR HYDROPHILIC INTERACTION (HILIC) CHROMATOGRAPHY Tohru Ikegami *, Hirotaka Takubo, Yuuta Watanabe, Kouki Tomomatsu, Kanta Horie,
Hiroshi Fujita, Nobuo Tanaka Department of Biomolecular Engineering, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan
17:10–17:25 L57 OPTIMIZATION OF PEAK CAPACITY IN ONE- AND TWO-DIMENSIONAL
NANOLC Sebastiaan Eeltink *
Dionex, Abberdaan 114, 1046AA, Amsterdam, The Netherlands 17:25–17:40 L58 MODIFIED CARBON MONOLITHIC COLUMNS FOR CHROMATOGRAPHIC
APPLICATIONS Ali Eltmimi *(1), Leon Barron (1), Pavel Nesterenko (2), Aran Rafferty (3), John
Hanrahan (4), Brett Paull (1) (1) National Centre for Sensor Research, School of Chemical Sciences, , Dublin City University, Glasnevin, Dublin 9, Ireland; (2) School of Chemistry, University of Tasmania, Australia; (3) School of Mechanical & Manufacturing Engineering, Dublin City University, Glasnevin, Dublin 9, Ireland; (4) Glantreo Ltd, BioTransfer-Unit, University College Cork, Ireland
Oral Session 14 CE and Microfluidics (Katsura Hall) (Chair: Stephen C. Jacobson, Huwei Liu) 16:10–16:30 L59 CAPILLARY ZONE ELECTROPHORESIS USING ELECTROKINETIC
SUPERCHARGING PRECONCENTRATION – THE STATE OF THE ART Takeshi Hirokawa *, Zhongqi Xu, Eiji Koshimidzu
Department of Applied Chemistry, Graduate School of Engineering, Hiroshima University,1-4-1 Kagamiyama, Higashi-hiroshima, Japan
16:30–16:50 L60 INVESTIGATIONS ON KINETICS OF BIOMOLECULES WITH CAPILLARY
ELECTROPHORESIS AND MICROFLUIDIC CHIP Youzhi Xu, Bifeng Liu *
Department of Systems Biology, College of Life Science & Technology, Huazhong University of Science and Technology, Wuhan 430074, China
16:50–17:10 L61 MICROSCALE ISOELECTRIC FOCUSING IN GLASS CHIPS Kiyohito Shimura *(1), Ryo Ishibashi (1), Yutaka Koyama (1), Takehiko Kitamori (1, 2)
(1) Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan; (2) Kanagawa Academy of Science and Technology, 3-2-1 Sakado, Takatsu, Kawasaki, Kanagawa 213-0012, Japan
17:10–17:25 L62 CAPILLARY ELECTROPHORESIS BASED DNA-PROTEIN BINDING ASSAY:
FROM BINDING AFFINITY TO BINDING DYNAMICS Hailin Wang *
State Key Laboratory of Environmental Chemistry, Research Center for Eco- Environmental Sciences, Chinese Academy of Sciences, Beijing , China 100085
17:25–17:40 L63 DOUBLE HIGH VOLTAGE POWER SUPPLY AND ITS APPLICATION IN
COMPLEX ELECTROOSMOTIC PUMP SYSTEM Huiyan You *(1, 2), Lihua Zhang (2), Yukui Zhang (2)
(1) Enviourmental and Chemical Engineering College, Dalian University, Dalian, 116622, China; (2) National Chromatographic R&A center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian, 116011, China
19:00–21:00 Banquet (Kyoto Royal Hotel & Spa)
Friday, December 5 Oral Session 15 Stationary Phases and Interactions (Funai Auditorium) (Chair: Yu-Qi Feng, Wolfgang Lindner) 9:15–9:40 L64 NEW LC CHIRAL SELECTORS: MECHANISM AND USE Daniel W. Armstrong *
Department of Chemistry and Biochemistry, University of Texas at Arlington, Arlington, TX 76019, USA
9:40–10:00 L65 WATER-ICE FOR DEVELOPING NOVEL ANALYTICAL METHODOLOGY Tetsuo Okada *, Yuiko Tasaki, Kohei Sugiya, Makoto Harada
Department of Chemistry, Tokyo Institute of Technology, Meguro-ku, Tokyo 152-8551, Japan
10:00–10:20 L66 HILIC - WHEN AND HOW? Knut Irgum *
Department of Chemistry, Umeå University, S-90187 Umeå, Sweden 10:20–10:40 L67 MIXED-MODE LIQUID CHROMATOGRAPHY: POTENTIAL AND
APPLICATIONS Michael Laemmerhofer *, Wolfgang Bicker, Wolfgang Lindner
Christian Doppler Laboratory of Molecular Recognition Materials, Department of Analytical Chemistry, University of Vienna, 1090 Vienna, Austria
10:40–11:00 Break Oral Session 16 Pharmaceutical and Life Science Applications (Funai Auditorium) (Chair: Michael Laemmerhofer, Hanfa Zou) 11:00–11:25 L68 DESIGN SPACE OF ANALYTICAL METHODS; AN EXTENSION OF ICH
QUALITY BY DESIGN CONCEPTS TO HPLC Fritz Erni * NOVARTIS Pharma, Group Quality Operations, CH-4002 Basel, Switzerland 11:25–11:45 L69 SPECIFIC ANALYSIS OF SMALL MOLECULE BINDING PROTEINS BY MASS
SPECTROMETRY COUPLED WITH AFFINITY EXTRACTION Nariyasu Mano * Department of Pharmaceutical Sciences, Tohoku University Hospital, 1-1 Seiryo-machi,
Aoba-ku, Sendai 980-8574, Japan 11:45–12:05 L70 ADVANCES OF HPLC AND HYPHENATION TECHNIQUES IN ANALYSIS OF
CHINESE HERBAL MEDICINES Ping Li *, Lian-Wen Qi Key Laboratory of Modern Chinese Medicines (China Pharmaceutical University),
Ministry of Education; Nanjing 210009, China 12:05–12:25 L71 TWO-DIMENSIONAL HPLC ANALYSIS OF D-AMINO ACIDS IN MAMMALS Kenji Hamase *(1), Yurika Miyoshi (1), Junzo Hirano (1), Hai Han (1), Akiko Morikawa
(1), Sachise Etoh (1), Yosuke Tojo (1, 2), Masashi Mita (2), Tsuneaki Kaneko (2), Yasuhiko Kadota (2), Wolfgang Lindner (3), Kiyoshi Zaitsu (1)
(1) Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; (2) Shiseido Co., Ltd., 1-1-16, Higashi-shimbashi, Minato-ku, Tokyo 105-0021, Japan; (3) Institute of Analytical Chemistry and Food
Chemistry, University of Vienna, Waehringerstrasse 38, A-1090 Vienna, Austria 12:30–13:45 Lunch (Lunch on own) Luncheon Seminar (12:30–13:30) Shiseido Co., Ltd. (Meeting Room 22AB of Funai Auditorium)
TBA Sigma-Aldrich Japan, K.K. (Meeting Room 34 of Funai Auditorium)
TBA Plenary Lectures–2 (Funai Auditorium) (Chair: John Frenz, Guowang Xu) 13:45–14:10 L72 INTEGRATED MICRO- AND NANOFLUIDIC DEVICES FOR SEPARATIONS Michelle L. Kovarik, Kaimeng Zhou, John M. Perry, Stephen C. Jacobson * Department of Chemistry, Indiana University, Bloomington, Indiana 47405-7102, USA 14:10–14:35 L73 HIGH PERFORMANCE CERAMIC CHROMATOGRAPHIC MICROFLUIDIC
DEVICES Steven A. Cohen *, James P. Murphy, Joseph Michienzi, Bernard Bunner, Geoff Gerhardt Waters Corporation, Milford MA USA 14:35–15:00 L74 WHAT DRIVES SELECTIVITY IN LIQUID CHROMATOGRAPHY Wolfgang Lindner *, Michael Laemmerhofer, Wolfgang Bicker
Department of Analytical Chemistry and Food Chemistry, University of Vienna, Waehringerstrasse 38, 1090 Vienna, Austria
15:00–15:15 Break Plenary Lectures–3 (Funai Auditorium) (Chair: Peter Schoenmakers, Nobuo Tanaka) 15:15–15:40 L75 SINGLE CELL NEUROMETABOLOMICS USING CAPILLARY SEPARATIONS Jonathan V. Sweedler *
Department of Chemistry, University of Illinois, Urbana IL 61801, USA 15:40–16:05 L76 MICROFLUIDICS FOR SINGLE BIOMOLECULE AND SINGLE CELL
ANALYSIS Yoshinobu Baba *(1–3) (1)Department of Applied Chemistry, Graduate School of Engineering, Department of
Advanced Medical Science, Graduate School of Medicine, MEXT Innovative Research Center for Preventive Medical Engineering, Plasma Nanotechnology Research Center, Nagoya University, Chikusa-ku, Nagoya 464-8603, Japan; (2)National Institute of Advanced Industrial Science and Technology (AIST); (3)Institute for Molecular Science, National Institute for Natural Sciences
16:05–16:30 L77 WHAT LC-MS METHODS DO WE NEED FOR METABONOMICS ANALYSIS? Guowang Xu *(1), Xinjie Zhao (1), Yuan Wang (1), Peiyuan Yin (1), Jing Chen (1), Jens
Fritsche (2), Xin Lu (1), Hans Ulrich Häring (2), Erwin D. Schleicher (2), Philippe Schmitt-Kopplin (3), Rainer Lehmann (2) (1) Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 16023 Dalian, China; (2) Department of Clinical Chemistry, Pathobiochemistry, Endocrinology and Metabolism; University Hospital Tuebingen, D-72076 Tuebingen, Germany; (3) GSF - National Research Center for Environment and Health Institute for Ecological Chemistry Ingoldstaedter Landstraße 1, D-85764 Neuherberg, Germany
16:30–16:40 Poster Award Presentations
16:40–16:50 Invitation to HPLC2009 Invitation to HPLC2010 16:50–17:00 Closing
Poster Presentation Program All posters should be put up on the poster boards by 11:00 AM on Wednesday, and should be removed on Friday between 15:00 and 17:30.
Poster Session 1 (PW), Wednesday, December 3, 12:30–14:30 (PW posters duty: 13:30–14:30)
CE, CEC, Applications
PW001 INVESTIGATION OF PROTEINS ANALYSIS WITH CAPILLARY ELECTROPHORESIS
UNDER LOW pH AND ADDING ORGANIC SOLVENT Songnan Liu (1), Feng Qu *(1), Xinying Zhao (2), Jinghua Zhang (2), Yulin Deng (1), Yukui Zhang
(3) (1) School of Life Science & Technology, Beijing Institute of Technology, Beijing 100081, China; (2)
Beijing Center for Physical and Chemical Analysis, Beijing 100089, China; (3) Dalian Institute of Chemical Physics, the Chinese Academy of Sciences, Dalian 116023, China
PW002 SUPPORTED PHOSPHOLIPID BILAYER COATINGS IN CAPILLARY
ELECTROCHROMATOGRAPHY FOR THE SEPARATION OF PROTEINS Ting-Yang Kuo*, Jung Hsieh, Liang-Gui Yu, Ja-an Annie Ho BioAnalytical Laboratory, Department of Chemistry, National Tsing Hua University, Hsin-chu, 30013
Taiwan PW003 SEPARATION OF METAL IONS AS COMPLEXES WITH 2,6-PYRIDINEDICARBOXYLIC
ACID BY CAPILLARY ELECTROPHORESIS N. Vachirapatama *(1), N. Theerapittayatakul (1), P. R. Haddad (2) (1) Department of Chemistry, Faculty of Science and Technology, Thammasat University, Rangsit,
Pathumthani, 12121, Thailand; (2) ACROSS, School of Chemistry, Faculty of Science and Engineering, University of Tasmania, GPO Box 252-75, Hobart, Tasmania, 7001, Australia
PW004 ELECTROPHORETIC ANALYSIS USING ORGANIC NANOCRYSTALS Hiroshi Sudaki *, Fumihiko Kitagawa, Koji Otsuka Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Katsura,
Nishikyo-ku, Kyoto 615-8510, Japan PW005 DEVELOPMENT OF A CE METHOD FOR THE SIMULTANEOUS DETERMINATION OF
ARIPIPRAZOLE AND ITS ACTIVE METABOLITE, DEHYDROARIPIPRAZOLE, IN HUMAN PLASMA
Pei-Li Huang *, Su-Hwei Chen Faculty of Pharmacy, College of Pharmacy, Kaohsiung Medical University, 100, Shih-Chuan 1st
Road, Kaohsiung 80708, Taiwan PW006 SIMULTANEOUS DETERMINATION OF GALANTHAMINE, RIVASTIGMINE AND NAP
226-90 BY CAPILLARY ELECTROPHORESIS: VALIDATION AND APPLICATION IN ALZHEIMER’S DISEASE
Ya-Hui Hsieh, Su-Hwei Chen * Faculty of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan PW007 SIMULTANEOUS DETERMINATION OF REGULAR INSULIN AND INSULIN ASPART BY
CAPILLARY ZONE ELECTROPHORESIS AND APPLICATION IN COMMERCIAL DRUG FORMULATIONS
Hsin-Hua Yeh (1), Su-Hwei Chen *(1, 2) (1) Faculty of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807,
Taiwan; (2) Center of Excellence for Environmental Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan
PW008 DETERMINATION OF METFORMIN IN PLASMA BY CAPILLARY ZONE
ELECTROPHORESIS WITH ULTRAVIOLET DETECTION FOR TYPE 2 DIABETIC MELLITUS
Shao-Yun Wei (1), Su-Hwei Chen *(1, 2) (1) Graduate Institute of Pharmaceutical Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan; (2) Department of Pharmacy, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung 807, Taiwan
PW009 SIMULTANEOUS DETERMINATION OF RUTIN AND QUERCETIN IN PROPOLIS BY
CAPILLARY ELECTROPHORESIS-CHEMILUMINESCENCE Jian-Quan Lu *, Na-Na Ma, Xing-Wang Zhou, Song Wang
Hubei Key Laboratory of Bioanalytical Technique, Hubei Normal University, Huangshi 435002, China
PW010 SIMULTANEOUS DETERMINATION OF CU AND CO ION IN CHINESE TRADITIONAL
MEDICINE BY MINIATURIZED CAPILLARY ELECTROPHORESIS WITH POST CHEMILUMINESCENCE
Jian-Quan Lu *, Jing Xu, Xing-Wang Zhou, Song Wang Hubei Key Laboratory of Bioanalytical Technique, Hubei Normal University, Huangshi 435002, China
PW011 STUDY OF DIHYDROFOLATE REDUCTASE INHIBITORS FROM EFFECTIVE
COMPONENTS IN CHINESE HERBAL MEDICINE BY CAPILLARY ELECTROPHORESIS
Xin-Yue Zhang, Ruo-Hua Zhu * Department of Chemistry, Capital Normal University, 100048, Beijing
PW012 DETERMINATION OF AMINO ACID COMPOSITION AND C-TERMINAL SEQUENCE
OF PEPTIDES BY CAPILLARY ZONE ELECTROPHORESIS Alena G. Mosina *(1), Igor O. Melnikov (1), Yuriy M. Glubokov (1), Igor V. Nazimov (2)
(1) Department of Analytical Chemistry, M.V. Lomonosov Moscow State Academy of Fine Chemical Technology, Vernadskogo pr., 86, Moscow, the Russian Federation 119571; (2) Laboratory of Protein Analytical Chemistry, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya Ul., 16/10, Moscow, the Russian Federation, 117997
PW013 CAPILLARY ELECTROPHORESIS WITH LASER-INDUCED FLUORESCENCE (CE-LIF)
MONITORS DISTRIBUTION OF DOXORUBICIN IN CHO-K1 CELLS Nein-Chu Fan *, Ja-an Annie Ho
BioAnalytical Laboratory, Department of Chemistry, National Tsing Hua University, Hsin-chu 30013 Taiwan
PW014 SENSITIVE DETERMINATION OF NITRIC OXIDE IN OTTELIA ALISMOIDES USING
CAPILLARY ELECTROPHORESIS WITH LASER-INTRODUCED FLUORESCENCE DETECTION
Zi-Xing Zhang, Li-Li Du, Hua-Shan Zhang, Hong Wang * Department of Chemistry, Wuhan University, Wuhan 430072, China
PW015 GENOTYPING OF SINGLE NUCLEOTIDE POLYMORPHISM IN MDM2 GENES BY
UNIVERSAL FLUORESCENCE PRIMER PCR AND CAPILLARY ELECTROPHORESIS Yen-Ling Chen (1), Jan-Gowth Chang (2, 3), Ya-Sian Chang (2, 3), Shou-Mei Wu *(1, 4)
(1) School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan; (2) Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; (3) Department of Laboratory Medicine, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung, Taiwan; (4) Department of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan
PW016 SIMULTANEOUS DETERMINATION OF EIGHT NUCLEOSIDE METABOLITES IN
URINE OF DIABETIC NEPHROPARTHY AND HEALTHY SUBJECTS BY CAPILLARY ELECTROPHORESIS
Tianxi He (1, 2), Qionglin Liang (1), Yiming Wang (1), Guoan Luo *(1)
(1) Key Lab of Bioorganic Phosphorus Chemistry & Chemical Biology of Ministry of Education, Tsinghua University, Beijing 100084, China; (2) Logistic Engineering of University; Chongqing 400016, China
PW017 QUANTIFICATION OF PHOSPHORUS IN NUCLEOTIDES USING CAPILLARY
ELECTROPHORESIS AND MICRO HPLC HYPHENATED WITH ICP MASS SPECTROMETRY
Shin-ichiro Fujii *, Kazumi Inagaki, Akiko Takatsu, Takashi Yarita, Koichi Chiba National Metrology Institute of Japan, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Umezono, Tsukuba, Ibaraki 305-8563, Japan
PW018 PARTIAL FILLING MODE OF CAPILLARY ZONE ELECTROPHORESIS FOR HUMAN
HIGH-DENSITY LIPOPROTEIN INTERACTION STUDIES Kati Vainikka *(1), Jie Chen (1), Matti Jauhiainen (2), Marja-Liisa Riekkola (1)
(1) Laboratory of Analytical Chemistry, Department of Chemistry, P.O. Box 55, FIN-00014 University of Helsinki, Finland; (2) National Public Health Institute, P.O.Box 104, FIN-00251 Helsinki, Finland
PW019 COMPARISON OF HPLC AND CE METHODS FOR THE DETERMINATION OF
MITOMYCIN C IN HUMAN PLASMA Yu-Wei Chou, Su-Hwei Chen *
Faculty of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan PW020 ON-LINE STACKING CAPILLARY ELECTROPHORESIS FOR ANALYSIS OF
METHOTREXATE AND ITS EIGHT METABOLITES IN WHOLE BLOOD Hui-Ling Cheng (1), Yu-Mei Liao (2), Shyh-Shin Chiou (2), Shou-Mei Wu *(1, 3)
(1) School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan; (2) Department of Pediatric, Kaohsiung Medical University, Kaohsiung, Taiwan; (3) Department of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan
PW021 DEVELOPMENT OF A STABILITY INDICATING CAPILLARY ELECTROPHORESIS
(CE) ASSAY FOR THE DETERMINATION OF CETIRIZINE AND HYDROXYZINE IN COMMERCIAL TABLETS
Ali Reza Fakhari *, Saeed Nojavan Department of Chemistry, Faculty of Sciences, Shahid Beheshti University, Evin, Tehran, Iran
PW022 ENZYME INHIBITOR SCREENING IN NATURAL EXTRACT BY CAPILLARY
ELECTROPHORESIS BASED ON ON-COLUMN ACTIVE ASSAY Jingwu Kang *, Zhongmei Tang, Tongdan Wang
Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China PW023 SELECTION OF APTAMER FOR MYOTONIC DYSTROPHY KINASE-RELATED
Cdc42-BINDING KINASE BY CAPILLARY ELECTROPHORESIS Junie Tok *(1), Jesyin Lai (1), Thomas Leung (2), Sam Fong Yau Li (1)
(1) Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore; (2) Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673, Singapore
PW024 IN-CAPILLARY DENATURING POLYMER ELECTROPHORESIS WITH
1,2,5-THIADIAZOLE AS A RNA DENATURANT Yoshinori Yamaguchi *(1, 2), Keiko Sumitomo (2), Takashi Ogura (1), Kuniaki Tatsuta (3)
(1) Consolidated Research Institute for Advanced Science and Medical Care (ASMeW), Waseda University, 513 Wasedatsurumaki-cho, Shinjuku-ku, Tokyo 162-0041, Japan; (2) SORST, Japan Science and Technology Agency (JST), Tokyo, Japan; (3) Department of Applied Chemistry, Faculty of Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku, Tokyo 169-8555, Japan
PW025 HIGHLY SENSITIVE ANALYSIS OF SUGAR CHAINS IN CAPILLARY
ELECTROPHORESIS
Takayuki Kawai *, Fumihiko Kitagawa, Koji Otsuka Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan
PW026 SIMUTANEOUS ULTRASENSITIVE DETERMINATION OF Co(II), Cu(II) BY A
MINIATURIZED CAPILLARY ELECTROPHORESIS SYSTEM WITH POST CHEMILUMINESCENCE DETECTION
Zhou Xingwang, Wang Song, Wang Ganglin, Lu Jianquan * College of Chemistry and Environmental Engineering, Hubei Normal University, Huangshi, P. R. China, 435002
PW027 LASER-INDUCED FLUORESCENCE DETECTOR FOR ARRAY CAPILLARY FLOW
SYSTEMS Jing Xu *, Shiheng Chen, Yan Xiong, Yafeng Guan
Department of Analytical Chemistry and Micro-Instrumentation, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
PW028 UNIVERSAL MULTIPLEX PCR AND CAPILLARY ELECTROPHORESIS FOR
QUANTIFICATION OF SMN1/SMN2 GENES IN SPINAL MUSCULAR ATROPHY Chun-Chi Wang (1), Jan-Gowth Chang (2–4), Shou-Mei Wu *(1, 4, 5), Yuh-Jyh Jong *(2, 3, 6)
(1) Faculty of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan; (2) Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; (3) Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan; (4) Center of Excellence for Environmental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; (5) Faculty of Fragrance and Cosmetics, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan; (6) Department of Pediatrics, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
PW029 HIGHLY SENSITIVE ANALYSIS OF INORGANIC IONS USING CROWN ETHER
IMMOBILIZED QUANTUM DOTS Minako Kai *(1), Shigeyoshi Horiike (2), Hiroaki Nakanishi (2), Fumihiko Kitagawa (1), Koji
Otsuka (1) (1) Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan; (2) Technical Research Laboratory, Shimadzu Corp., 3-9, Hikaridai, Seika-cho, Soraku-gun, Kyoto 619-0237, Japan
PW030 SEPARATION OF SMALL MOLECULAR PEPTIDES BY CE AND LC WITH ESI-MS
DETECTION Zilin Chen *, Yue Wu, Lihong Liu, Jun Xue
Department of Pharmaceutical Analysis, College of Pharmacy, Wuhan University, Wuhan 430072, China
PW031 COMPARISON OF HPLC AND CE METHODS FOR THE DETERMINATION OF FREE
FORM AND TOTAL FORM OF PHENYTOIN IN HUMAN PLASMA SAMPLES AND THEIR CLINICAL APPLICATIONS
Ping-Chih Lin, Su-Hwei Chen * Faculty of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan
PW032 ANALYSIS OF REACTANT AND PRODUCT ON BAYLIS-HILLMAN REACTION BY
MEKC Li Qi *(1), Kai Cui (1, 2), Juan Qiao (1, 2), Jun Yang (1, 2)
(1) Beijing National Laboratory of Molecular Science; Laboratory of Analytical Chemistry for Life Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China; (2) Graduate School, Chinese Academy of Sciences, Beijing 100049, China
PW033 IONIC-STRENGTH RESPONSIVE COMPOSITE PNIPAAM-ASSISTED MEKC FOR
NEUTRAL STEROIDS SEPARATION Li-Chen Wu *, Ching-Yuan Hu
Department of Applied Chemistry, National Chi Nan University, Nantou, Puli, 545, Taiwan
PW034 MIXTURE DESIGN IN THE OPTIMISATION OF A MEEKC METHOD FOR THE
DETERMINATION OF OXYBUTYNIN AND ITS IMPURITIES IN DRUG PRODUCTS Sandra Furlanetto *, Iacopo Giannini, Serena Orlandini, Sergio Pinzauti
Department of Pharmaceutical Sciences, University of Florence, Via U. Schiff 6, 50019 Sesto Fiorentino (FI), Italy
PW035 TRANSIENT-TRAPPING IN MEKC FOR HIGH-SENSITIVE DETECTION Kota Hashiba *, Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka
Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan
PW036 SWEEPING OF BIOGENIC COMPOUNDS IN CYCLODEXTRIN ELECTROKINETIC
CHROMATOGRAPHY Takuya Saito *, Fumihiko Kitagawa, Koji Otsuka
Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan
PW037 STRATEGIES FOR CE-SDS PEAK CHARACTERIZATION Katariina M. Hutterer *, Dingjiang Liu, Anil Raghani
Analytical and Formulation Sciences, Amgen Inc., One Amgen Center Drive, Thousand Oaks, CA 91320-1799, USA
PW038 HIGH-SENSITIVITY CE-AD ANALYSIS OF FOUR DOPING IN HUMAN URINE WITH
ON-LINE ISOTACHOPHORESIS SAMPLE STACKING Lihui Zheng, Ping Tong, Guonan Chen, Lan Zhang *
Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety (Fuzhou University), Department of Chemistry, Fuzhou University, Fuzhou 350002, Fujian, China; Sport Science Research Center, Fuzhou University, Fuzhou 350002, Fujian, China
PW039 CAPILLARY ELECTROPHORESIS WITH PRE-CAPILLARY COMPLEXATION
TECHNIQUE FOR ANALYSIS OF URANIUM IN RADIOACTIVE WASTES Tomoko Haraga *(1, 2), Atsushi Katayama (1), Yutaka Kameo (1), Mikio Nakashima (1), Toru
Takahashi (2), Nobuhiko Iki (2), Hitoshi Hoshino (2) (1) Nuclear Cycle Backend Directorate, Japan Atomic Energy Agency, Shirane, Shirakata, Tokai-mura, Naka-gun, Ibaraki 319-1195, Japan; (2) Graduate School of Environmental Studies, Tohoku University, Aoba, Aramaki, Aoba-ku, Sendai-shi, Miyagi 980-8579, Japan
PW040 VOLTAGE-INDUCED ZONE SHARPENING FOR PEPTIDES IN PRESSURIZED-FLOW
DRIVEN HYDROPHILIC INTERACTION CAPILLARY ELECTROCHROMATOGRAPHY USING MOBILE PHASE CONTAINING PERCHLORIC ACID
Shinya Kitagawa *, Koichi Sakabe, Hiroki Buno, Hajime Ohtani Department of Materials Sciences and Engineering, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso, Showa, Nagoya 466-8555, Japan
PW041 CEC-ESI-MS FOR RAPID AND SENSITIVE ANALYSIS OF DOPING Minghua Lu *, Qiang Feng, Qiaomei Lu, Guonan Chen, Lan Zhang *
Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety (Fuzhou University), Department of Chemistry, Fuzhou University, Fuzhou 350002, Fujian, China; Sport Science Research Center, Fuzhou University, Fuzhou 350002, Fujian, China
PW042 SUBNANOMOLAR ASSAY OF RECOMBINANT HUMAN ERYTHROPOIETIN BY
CAPILLARY ELECTROPHORESIS USING PRECOLUMN LABELING WITH NANOORANGE AND LASER-INDUCED FLUORESCENCE DETECTION
Nannan Pang, Yu Zhou, Xia Yang, Zhengxiang Zhang, Honggang Nie, Xiaofang Fu, Huwei Liu * Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
PW043 FITC AS LABEL FOR THE CHARACTERIZATION OF PAMAM- BASED OPEN TUBULAR COLUMN FOR CAPILLARY ELECTROCHROMATOGRAPHY PREPARED BY MICROWAVE IRRADIATION
Hongjun E, Ping Su, Qi Zhou, Siming Wang, Yi Yang * College of Science, Beijing University of Chemical Technology, Beijing 100029, China
Microchip Technologies PW044 ANALYSIS OF THE MYROSINASE-GLUCOSINOLATES SYSTEM: A CHIP BASED
TECHNOLOGY FOR PROTEOMICS AND METABOLOMICS RESEARCH Maged Fouad *(1, 2), Noritada Kaji (1, 2), Yukihiro Okamoto (1, 2), Manabu Tokeshi (1, 2),
Yoshinobu Baba (1–4) (1) Department of Applied Chemistry, Graduate School of Engineering; (2) MEXT Innovative
Research Center for Preventive Medical Engineering; (3) Plasma Nanotechnology Research Center, Nagoya University, Nagoya 464-8603, Japan; (4) National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu 761-0395, Japan
PW045 ELECTROPHORETIC ANALYSES OF BIOGENIC COMPOUNDS USING SURFACE
MODIFIED POLYMER MICROCHIPS Fumihiko Kitagawa *(1), Kei Kubota (1), Hidetoshi Shinohara (2), Yoshikazu Takahashi (3), Jun
Mizuno (2), Shuichi Shoji (2), Koji Otsuka (1) (1) Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Katsura,
Nishikyo-ku, Kyoto 615-8510, Japan; (2) Major in Nano-Science and Nano-Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan; (3) SO-KEN, 3-5-4 Shibakouen, Minato-ku, Tokyo 107-0011, Japan
PW046 RESHAPING MICROFABRICATED COLUMNS FOR CEC: FOIL-SHAPED PILLARS Sertan Sukas *, Han Gardeniers Mesoscale Chemical Systems, MESA+ Institute for Nanotechnology, University of Twente, Enschede,
The Netherlands PW047 THE APPLICATION RESEARCH OF VALVE-LESS MICROPUMP FOR MICROFLUIDIC
CHIP Wei Wang *, Ying Zhang, Li Tian, Hai-Feng Zhang, Yu Song, Xiao-Wei Liu MEMS Center, Harbin Institute of Technology, Harbin, 150001, China PW048 PERFORMANCE ANALYSIS OF MICROCHIP ELECTROPHORESIS FABRICATED BY
THE PRECISION CNC MILLING Tian Li *, Wang Wei, Liu Xiaowei, Li Ling, Song Yu, Lu Shaoyong, Hu Jun MEMS Center, School of Astronautics, Harbin Institute of Technology, No.92 Nangang District
Harbin Heilongjiang Province, China PW049 HIGH PERFORMANCE ITP-CE IN MICROFLUIDIC IMMUNOASSAY SYSTEM L. Bousse (1), W. W. P. Chang (1), R.L. Chien *(1), C. Li (1), C. Kagebayashi (2), T. Kawabata (2), T.
Kurosawa (2), H.G. Wada (1), M. Watanabe (2), S. Satomura (2) (1) Mountain View R&D Center, Wako Pure Chemical Industries, Ltd., Mt. View, California, USA; (2)
New Diagnostics Business and Technology Development Department, Wako Pure Chemical Industries, Ltd., Osaka, Japan
PW050 SURFACE MODIFICATION METHOD OF THE MICROCHANNELS FOR
BIOMOLECULES’ ANALYSIS Yukihiro Okamoto *(1, 2), Yeon-Su Park (2), Noritada Kaji (1, 2), Manabu Tokeshi (1, 2), Yoshinobu
Baba (1–3) (1) Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Furo- cho, Chikusa-ku, Nagoya 464-8603, Japan; (2) MEXT Innovative Research Center for Preventive Medical Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan; (3) National Institute of Advanced Industrial Science and Technology, Hayashi-cho 2217-14, Takamatsu 761-0395, JAPAN(1) Mountain View R&D Center, Wako Pure Chemical Industries, Ltd., Mt. View,
California, USA; (2) New Diagnostics Business and Technology Development Department, Wako Pure Chemical Industries, Ltd., Osaka, Japan
PW051 DEVELOPMENTS OF NANOPILLAR CHIPS FOR FAST DNA AND PROTEIN
SEPARATIONS Takao Yasui (1), Noritada Kaji *(1, 2), Mohamad Reza Mohamadi (3), Ryo Ogawa (4), Shingi
Hashioka (4), Manabu Tokeshi (1, 2), Yasuhiro Horiike (4), Yoshinobu Baba (1, 2, 5) (1) Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Nagoya
464-8603, Japan; (2) MEXT Innovative Research Center for Preventive Medical Engineering, Nagoya University, Nagoya 464-8603, Japan; (3) Institute Curie, UMR CNRS 168, Paris, France; (4) National Institute for Materials Science, Tsukuba 305-0044, Japan; (5) Health Technology Research Center, National Institute of Advanced Industrial Science and Technology, Takamatsu 761-0395, Japan
PW052 A MICROCHIP ELECTROPHORESIS METHOD DEVELOPED FOR
GLYCOSAMINOGLYCAN DISACCHARIDE ISOMER SEPARATION Yong Zhang *(1), Yukihiro Okamoto (2), Noritada Kaji (1, 2), Manabu Tokeshi (1, 2), Yoshinobu
Baba (1–5) (1) Department of Applied Chemistry, Graduate School of Engineering; (2) MEXT Innovative
Research Center for PME; (3) Plasma Nanotechnology Research Center, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, JAPAN; (4) AIST, Hayashi-cho 2217-14, Takamatsu 761-0395, JAPAN; (5) Institute for Molecular Science, National Institutes of Natural Sciences, Myodaiji Nishigo-naka 38, Okazaki 444-8585, JAPAN
PW053 A RAPID AND EASY DETECTION OF QUINOLONE-RESISTANT ESCHERICHIA COLI
BY MICROCHIP GEL ELECTROPHORESIS Eri Yoshikawa *(1) ,Noritada Kaji (1, 2), Risa Yoshida (3), Kumiko Kawamura (2, 3), Yukihiro
Okamoto (1, 2), Manabu Tokeshi (1, 2), Michio Ohta (2, 4), Yoshinobu Baba (1, 2, 5) (1) Department of Applied Chemistry, Graduate School of Engineering, Nagoya Univ., Japan; (2)
MEXT Innovative Research Center for Preventive Medical Engineering, Nagoya Univ., Japan; (3) School of Health Sciences, Nagoya Univ., Japan; (4) Graduate School of Medicine, Nagoya Univ., Japan; (5) Plasma Nanotechnology Research Center, Nagoya Univ., Japan; Health Technology Research Center, AIST, Japan; Institute for Molecular Science, Japan
PW054 DEVELOPMENT OF MICROFLUDIC DEVICES INTEGRATING HIGHLY SENSITIVE
FET SENSOR Kei Takemura *, Fumihiko Kitagawa, Koji Otsuka Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Katsura,
Nishikyo-ku, Kyoto 615-8510, Japan PW055 MICROCHIP ELECTROPHORETIC ANALYSIS USING MAGNETIC FLUID Takafumi Samukawa *, Fumihiko Kitagawa, Koji Otsuka Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Katsura,
Nishikyo-ku, Kyoto 615-8510, Japan
Chiral Separations PW056 ENANTIOSEPARATION DETERMINATION OF PENEQUININE HYDROCHLORIDE IN
HUMAN PLASMA Xiang Jin, Yu Qin, Liang Mao-Zhi *, Qin Yong-Ping, Nan Feng Department of Clinical Pharmacology, West China Hospital, S.C.U, Chengdu, 610041, P.R.China PW057 SEPARATION OF R- AND S-BINAPHTHOL WITH PARA-SULFONATE CALIX[6]ARENE
AS ADDITIVE BY NONAQUEOUS CAPILLARY ELECTROPHORESIS Yulu Tian, Kai Hu, Yuan Cheng, Baoxian Ye, Yangjie Wu, Shusheng Zhang * Chemistry Department, Key Laboratory of Chemical Biology and Organic Chemistry of Henan,
Zhengzhou University, Zhengzhou 450052, China
PW058 CHIRAL SEPARATION OF GEMIFLOXACIN IN MICELLE ELECTROKINETIC CHROMATOGRAPHY WITH CHIRAL CROWN ETHER AS CHIRAL ADDITIVE
Dongri Jin *, Shaodong Jia Key Laboratory of Organism Functional Factors of the Changbai Mountain, Ministry of Education,
Yanbian University, Yanji 133002, China PW059 ENANTIOMERIC SEPARATION OF dl-METHYLPHENIDATE (RITALIN) IN HUMAN
PLASMA BY FIELD AMPLIFIED SAMPLE STACKING WITH CAPILLARY ELECTROPHORESIS
Shih-Chieh Lee *(1), Shou-Mei Wu (1, 2) (1) School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan;
(2) Department of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan
PW060 ABOUT THE MECHANISM OF CONCENTRATION-DEPENDENT REVERSAL OF
ENANTIOMER MIGRATION ORDER OF SOME CHIRAL IMIDAZOLE DERIVATIVES IN CAPILLARY ELECTROPHORESIS
Ketevan Lomsadze *(1), Ana B. Martinez-Giron (2), Maria Castro-Puyana (2), Antonio L. Crego (2), Maria Luisa Marina (2), Antonio Salgado (3), Bezhan Chankvetadze (1)
(1) Department of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, Chavchavadze Ave 1, 0128 Tbilisi, Georgia; (2) Department of Analytical Chemistry, Faculty of Chemistry, University of Alcala, 28871 Alcala de Henares (Madrid), Spain; (3)Department of Medicinal Chemistry, Centro National de Investigaciones Oncologicas (CNIO), 28029 Madrid, Spain
PW061 ENANTIOMERIC SEPARATION OF DIPEPTIDES BY CE-ESI-MS EMPLOYING A
PARTIAL FILLING TECHNIQUE WITH A CHIRAL CROWN ETHER Shifei Xia *, Lan Zhang*, Ping Tong*, Wei Liu, Guonan Chen* Ministry of Education Key Laboratory of Analysis and Detection for Food Safety (Fuzhou University), Department of Chemistry, Fuzhou University, Fuzhou 350002, Fujian, China; Sport
Science Research Center, Fuzhou University, Fuzhou 350002, Fujian, China PW062 DYNAMICALLY COATING CHIRAL IONIC LIQUID CAPILLARY ELECTROPHORESIS
FOR ENANTIOSEPARATION OF HESPERETIN AND NARINGENIN Shu-Ping Wang, De-Lun Chiu * Department of Applied Chemistry, Providence University, 200 Chung Chi Rd., Shalu, Taichung Hsien
43301, Taiwan PW063 CHIRAL SEPARATION OF THE ENANTIOMERS OF TENATOPRAZOLE AND
RELATED SULFOXIDES USING CHIRAL LIGAND-EXCHANGE CHROMATOGRAPHY AND ITS MECHANISM RESEARCH
Jin Guan *(1, 2), Hengda Gu (2), Shuang Shi (2), Famei Li (1) (1) Department of Analytical Chemistry, Shenyang Pharmaceutical University, Shenyang 110016, PR
China; (2) School of Applied Chemistry, Shenyang Institute of Chemical Technology, Shenyang 110142, PR China
PW064 ENANTIOSEPARATION AND IMPURITY DETERMINATION OF LACTOFEN
ENANTIOMERS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY ON AMYLOSE STATIONARY PHASE
Feng Yan *(1, 2), Yongjie Chen (1), Jin Guan (1), Xiaoxia Liu (2) (1) School of Applied Chemistry, Shenyang Institute of Chemical Technology, Shenyang 110142, PR
China; (2) College of Sciences, Northeastern University, Shenyang 110004, PR China PW065 COMPARISON OF ENANTIOSELECTIVE SEPARATION OF PROFEN NSAIDS AND
β-BLOCKERS ON CHIROBIOTIC T AND CHIROBIOTIC T2 COLUMNS Zuzana Bosáková *(1), Marie Honetschlägerová - Vadinská (1), Eva Tesařová (2), Pavel Coufal (1) (1) Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Albertov
2030, 128 40 Prague 2, Czech Republic; (2) Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University in Prague, Albertov 2030, 128 40 Prague 2, Czech Republic
PW066 PREPARATION OF A NEW CHIRAL STATIONARY PHASE CONTAINING THIOL ESTER
LINKAGE BASED ON (+)-(18-CROWN-6)-2, 3, 11, 12-TETRACARBOXYLIC ACID AND THE APPLICATION
Hwan Sun Cho, Hee Jung Choi, Myung Ho Hyun * Department of Chemistry, Pusan National University, Busan, Korea PW067 EFFECTS OF ACID AND ORGANIC SOLVENT COMPOSITIONS ON THE ENANTIOMER
SEPARATION OF NBD-AMINO ACIDS USING PIRKLE TYPE AND ANION EXCHANGE TYPE CHIRAL STATIONARY PHASES
Takaaki Tanaka *(1), Kenji Hamase (1), Masashi Mita (2), Wolfgang Lindner (3), Kiyoshi Zaitsu (1) (1) Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku,
Fukuoka 812-8582, Japan; (2) Shiseido Co., Ltd., 1-1-16, Higashi-shimbashi, Minato-ku, Tokyo 105-0021, Japan; (3) Institute of Analytical Chemistry and Food Chemistry, University of Vienna, Waehringerstrasse 38, A-1090 Vienna, Austria
PW068 2D-HPLC SEPARATION OF CYSTEINE ENANTIOMERS AFTER FLUORESCENCE
DERIVATIZATION WITH NBD-F Junzo Hirano (1), Kenji Hamase *(1), Kyoko Ueno (1), Takaaki Tanaka (1), Yosuke Tojo (1, 2),
Masashi Mita (2), Wolfgang Lindner (3), Kiyoshi Zaitsu (1) (1) Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku,
Fukuoka 812-8582, Japan; (2) Shiseido Co., Ltd., 1-1-16, Higashi-shimbashi, Minato-ku, Tokyo 105-0021, Japan; (3) Institute of Analytical Chemistry and Food Chemistry, University of Vienna, Waehringerstrasse 38, A-1090 Vienna, Austria
PW069 RESOLUTION OF RACEMIC FLECAINIDE AND ITS ANALOGUES ON CHIRAL
STATIONARY PHASE (CSP) BASED ON (+)-(18-CROWN-6)-2,3,11,12- TETRACARBOXYLIC ACID
A Reum Lee *, Su Jin Lee, Hee Jung Choi, Myung Ho Hyun Department of Chemistry, Pusan National University, Busan, KOREA PW070 A SCREENING STUDY OF CHIRAL PHARMACEUTICALS USING TRIS(4-CHLORO-3-
METHYLPHENYLCARBAMATE) OF CELLULOSE-BASED STATIONARY PHASE AND POLAR ORGANIC MOBILE PHASES
Katina Sylvestre Dossou (1), Patrice Chiap (2), Anne-Catherine Servais (1), Marianne Fillet (1), Jacques Crommen *(1)
(1) Laboratory of Analytical Pharmaceutical Chemistry, Bioanalytical Chemistry Research Unit, Dept. of Pharmaceutical Sciences, University of Liege, CHU, B36, B-4000 Liege, Belgium; (2) Advanced Technology Corporation (A.T.C.), University Hospital Centre of Liege, Liege, Belgium
PW071 MICROFLUIDIC OPEN TUBULAR CAPILLARY ELECTROCHROMATOGRAPHY FOR
RAPID CHIRAL RECOGNITION Guisen Zhang *, Bifeng Liu Department of Systems Biology, College of Life Science & Technology, Huazhong University of
Science and Technology, Wuhan 430074, China
Novel Chromatographic Phases and Columns PW072 FUNCTIONALIZED MAGNETIC MICROPARTICLES AS SORBENTS EXTRACT
ORGANIC POLLUTANTS FROM ENVIRONMENTAL SAMPLES BY MAGNETIC SEPARATION FOLLOWED BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Yongsheng Ji (1), Xiaoyan Liu (1), Min Guan (1), Haixia Zhang *(1, 2), Chunming Wang (1) (1) College of Chemistry and Chemical engineering, Lanzhou University, Lanzhou, China, 730000;
(2) State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China PW073 PREPARATION OF MONODISPERSED VINYLPYRIDINE-DIVINYLBENZENE POROUS
COPOLYMER RESINS AND THEIR APPLICATION TO HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC SEPARATION OF AROMATIC AMINES
Kei-ichi Kitahara (1), Shuji Okuya (2), Kunio Nagashima (2), Takako Hanada (1), Isao Yoshihama (3), Sadao Arai *(1)
(1) Department of Chemistry, Tokyo Medical University, Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan; (2) Department of Applied Chemistry, Kogakuin University, Nishishinjuku, Shinjuku-ku, Tokyo 163-8677, Japan; (3) Electron Microscopy Laboratory, Tokyo Medical University, Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan
PW074 A NEWLY DEVELOPED HYDROPHILIC POLYMER-BASED ION EXCHANGE
CHROMATOGRAPHY COLUMN FOR SEPARATION OF VARIOUS BIOLOGICAL MOLECULES
Noriko Shoji *, Akiko Matsui, Masakatsu Omote, Naohiro Kuriyama YMC Co., Ltd., Kokufudai, Komatsu 923-8557, Japan PW075 ADVANTAGES OF ULTRA-FAST LIQUID CHROMATOGRAPHY USING 2 MICRON
PACKING MATERIALS Noriko Shoji *, Chie Yokoyama, Takashi Sato, Masakatsu Omote, Naohiro Kuriyama YMC Co., Ltd., Kokufudai, Komatsu 923-8557, Japan PW076 DEVELOPMENT OF FIBER-PACKED CAPILLARY IN MICROCOLUMN LIQUID
CHROMATOGRAPHY Ikuo Ueta *, Kenichi Nakane, Akira Abe, Yoshihiro Saito, Kiyokatsu Jinno School of Materials Science Toyohashi University of Technology, Toyohashi 441-8580, Japan PW077 NOVEL SEPARATION MEDIA FOR HIGH THROUGHPUT SEPARATION Fuminori Watanabe *, Takuya Kubo, Kunimitsu Kaya, Ken Hosoya Graduate School of Environmental Studies, Tohoku University, Aoba 6-6-20, Aramaki, Aobaku,
Sendai 980-8579, Japan PW078 CHARACTERIZATION OF A HIGHLY INERT ODS COLUMN FOR HPLC Kensuke Okusa *(1), Yuki Suita (1), Yukio Otsuka (2), Mineo Tahara (2), Masayoshi Ohira (1),
Masakazu Takahashi (1) (1) GL Sciences Inc., 237-2 Sayamagahara, Iruma, Saitama, 358-0032, Japan; (2) GL Sciences Inc.,
5-3 Nagasone, Okajima, Fukushima, 960-8201, Japan PW079 PERFORMANCE OF C18 STATIONARY PHASE PREPARED BY NEW END-CAPPING
METHOD Hiroshi Sakamaki *, Yoshihisa Sudo Chemicals Evaluation and Research Institute, Japan, Shimotakano, Sugito-machi, Kitakastushika- gun, Saitama, 345-0043, Japan PW080 FAST PROTEIN DIGESTION AND IDENTIFICATION USING HYBRID SILICA
MONOLITH BASED METAL-ION CHELATELY IMMOBILIZED TRYPSIN MICROREACTOR AND μHPLC-MS/MS
Junfeng Ma, Zhen Liang *, Lihua Zhang, Weibing Zhang, Yukui Zhang National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of
Sciences, Dalian 116023, China PW081 GREENER MICELLAR LIQUID CHROMATOGRAPHY INCORPORATING WITH SHORT
COLUMN C18 FOR DETERMINATION OF PARABENS IN THAI COMMUNITY COSMETICS
Thanatcha Chaida *, Napaporn Youngvises Department of Chemistry, Faculty of Science and Technology, Thammasat University, Pathum Thani,
12121, Thailand PW082 A NOVEL PROTEIN A AFFINITY CHROMATOGRAPHIC MEDIUM FOR
HIGH-THROUGHPUT PURIFICATION WITH REDUCED NON-SPECIFIC PROTEIN ADSORPTION
Katsuyuki Maeno *, Aya Hirayama, Kenichi Sakuma, Kazuyuki Miyazawa SHISEIDO Research Center, 2-2-1 Hayabuchi, Tsuzuki, Yokohama, Japan 224-8558
PW083 PEPTIDE ANALYSIS BY HYDROPHILIC INTERACTION CHROMATOGRAPHY –
IT/TOF MASS SPECTROMETER (HILIC-LCMS-IT-TOF) ON SUB 2-3 µM SILICA COLUMNS
Tatsunari Yoshida *(1), Satoshi Yamaki (1), Tadayuki Yamaguchi (1), Tetsuo Iida (2), Hirohisa Mikami (2)
(1) Analytical Applications Department, Shimadzu Corporation, Horiyamashita, Hadano, Kanagawa 259-1304, Japan; (2) Analytical Applications Department, Shimadzu Corporation, Nishinokyo, Nakagyo-ku, Kyoto 604-8511, Japan
PW084 PREPARATION OF METHACRYLATE-BASED MONOLITHIC STATIONARY PHASES
FOR HIGH THROUGHPUT SEPARATION USING UV PHOTOPOLYMERIZATION Tomohiko Hirano *, Shinya Kitagawa, Hajime Ohtani Department of Material Engineering, Graduate School of Engineering, Nagoya institute of
Technology, Gokiso-cho, Showa-ku, Nagoya-shi, Aichi 466-8555, Japan PW085 PREPARATION OF MONOLITHIC SILICA COLUMNS FOR PURIFICATION OF
VARIOUS ANTIBODIES Takashi Osaki *(1), Hiroyoshi Minakuchi (1), Kiyonori Hirota (2), Shinya Honda (2), Masahiro
Iwakura (2) (1) Kyoto Monotech Co., Creation Core Kyoto Mikuruma, Kamigyo-ku, Kyoto 602-0841, Japan; (2)
National Institute of Advanced Industrial Science and Technology (AIST), AIST Tsukuba Central 1, Tsukuba, Ibaraki 305-856, Japan
PW086 SPONTANEOUS SOLVENT TRANSPORT BEHAVIOR IN MACRO/MESOPOROUS SILICA
MONOLITHS Kazuki Nakanishi *(1), Kei Morisato (2), Chiemi Ohnishi (1), Naobumi Oi (3) (1) Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa,
Sakyo-ku, Kyoto 606-8502, Japan; (2) GL Sciences Inc. / Kyoto University, 12, 14 Yoshida Kawara-machi, Sakyo-ku, Kyoto 606-8305, Japan; (3) Chiralchromatography Laboratory, 50-29, Ogura-cho, Kitashirakawa, Sakyo-ku, Kyoto 615-8264, Japan
PW087 HIGH-POROSITY MACRO/MESOPOROUS SILICA MONOLITHS APPLIED TO HPLC
SEPARATION MEDIUM Takanori Detani *(1), Kazuki Nakanishi (1), Kei Morisato (2), Kazuyoshi Kanamori (1), Teiichi
Hanada (1) (1) Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa,
Sakyo-ku, Kyoto 606-8502, Japan; (2) GL Sciences Inc. / Kyoto Univ., 12, 14 Yoshida Kawaramachi, Sakyo-ku, Kyoto 606-8305, Japan
PW088 VARIETY SIZE OF MONOLITHIC COLUMN FOR CAPILLARY HIGH PERFORMANCE
LIQUID CHROMATOGRAPHY Masanori Motokawa *(1), Akinori Honda (2), Masayoshi Ohira (1), Hiroyoshi Minakuchi (3) (1)237-2 Sayamagahara, Iruma, Saitama, 358-0032 Japan, GL Sciences Inc.; (2)5-3 Nagasone,
Okazima, Fukusima, 960-8201 Japan, GL Sciences Inc.; (3)448-5 Kaziicho, Imadegawa, Kawaramachi, Kami-kyoku, Kyoto, 602-0841 Japan, Kyoto Monotech Co.
PW089 HIGH EFFICIENT SEPARATION OF PROTEINS USING A MONOLITHIC
WIDE-POROUS SILICA COLUMN Hironobu Morisaka *(1), Masahiro Furuno (2), Hiroyoshi Minakuchi (3), Kazuki Nakanishi (4),
Mitsuyoshi Ueda (1) (1) Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University,
Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan; (2) GL Sciences Inc., 237-2 Sayamagahara, Iruma, Saitama 358-0032, Japan; (3) Kyoto Monotech Co., 448-5 Kajii-cho, Kamigyo-ku, Kyoto 602-0841, Japan; (4) Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan
PW090 SEMI-MICRO MONOLITHIC COLUMNS USING MACROPOROUS SILICA RODS WITH
IMPROVED PERFORMANCE
Kei Morisato *(1), Shota Miyazaki (2), Masayoshi Ohira (2), Masahiro Furuno (2), Masahiko Nyudo (3), Hiroyuki Terashima (3), Kazuki Nakanishi (4)
(1) GL Sciences Inc. / Kyoto Univ., 12, 14 Yoshida Kawaramachi, Sakyo-ku, Kyoto 606-8305, Japan; (2) GL Sciences Inc., 237-2 Sayamagahara, Iruma, Saitama, 358-0032, Japan; (3) GL Sciences Inc. ,5-3 Nagasone, Okajima, Fukushima, 960-8201, Japan; (4) Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan
PW091 INFLUENCE OF STRUCTURAL DIFFERENCES ON DIGESTION EFFICIENCY IN
TRYPSIN-IMMOBILIZED MONOLITHIC SILICA SPIN COLUMN Shigenori Ota *(1), Masayoshi Ohira (1), Kei Morisato (2), Kazuki Nakanishi (3) (1) GL Sciences Inc., 237-2 Sayamagahara, Iruma, Saitama, 358-0032, JAPAN; (2) GL Sciences Inc.
/ Kyoto Univ., 12, 14 Yoshida Kawaramachi, Sakyo-ku, Kyoto 606-8305, Japan; (3) Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan
PW092 DEVELOPMENT OF MICRO-VOLUME SPIN COLUMN USING MONOLITHIC SILICA
MODIFIED WITH BORONIC ACID AS PURIFICATION TOOL OF CATECHOLAMINES Shigenori Ota *(1), Tomoko Yamada (1), Masayoshi Ohira (1), Kei Morisato (2), Kazuki Nakanishi
(3) (1) GL Sciences Inc., 237-2 Sayamagahara, Iruma, Saitama, 358-0032, JAPAN; (2) GL Sciences Inc.
/ Kyoto Univ., 12, 14 Yoshida Kawaramachi, Sakyo-ku, Kyoto 606-8305, Japan; (3) Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan
PW093 SELECTIVITY COMPARISON OF MONOLITHIC SILICA CAPILLARY COLUMNS
MODIFIED WITH POLY(OCTADECYL METHACRYLATE) AND OCTADECYL MOIETY FOR HALOGENATED COMPOUNDS IN REVERSED-PHASE HPLC
Wasura Soonthorntantikul *(1), Natchanun Leepipatpiboon (1), Tohru Ikegami (2), Nobuo Tanaka (2), Thumnoon Nhujak (1) (1) Chromatography and Separation Research Unit, Department of Chemistry, Faculty of Science,
Chulalongkorn University, Patumwan, Bangkok, 10330, Thailand; (2) Department of Biomolecular Engineering, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan
PW094 DEVELOPMENT OF HIGH-EFFICIENCY ION-EXCHANGE MONOLITHIC SILICA
COLUMNS Yuta Watanabe *(1), Jafariah Jaafar (2), Kanta Horie (1), Takeshi Hara (1), Tohru Ikegami (1), Nobuo
Tanaka (1) (1) Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku,
Kyoto, 606-8585, Japan; (2) Department of Chemistry, Faculty of Science, University of Technology Malaysia, UTM Skudai, Malaysia
PW095 STUDY OF PEAK CAPACITY UTILIZING MONOLITHIC SILICA CAPILLARY
COLUMNS IN LIQUID CHROMATOGRAPHY – MASS SPECTROMETRY Hirotaka Takubo *(1), Satoshi Makino (1), Takefumi Kawabe (2), Yasushi Ishihama (3), Tohru
Ikegami (1), Nobuo Tanaka (1) (1) Department of Biomolecular Engineering, Graduate School of Science and Technology, Kyoto
Institute of Technology, Kyoto, 606-8585, Japan; (2) Daiichisankyo.Co., Hiratsuka, Japan; (3)
Institute for Advanced Biosciences, Keio University, 403-1, Daihoji, Tsuruoka, Yamagata 997-0017, Japan
PW096 EVALUATION OF METHANOL-WATER AND ACETONITRILE-WATER BINARY
MIXTURES AS THE ELUENTS FOR TEMPERATURE-DEPENDENT INCLUSION CHROMATOGRAPHY
E. Włodarczyk *(1), P.K. Zarzycki (1), D.W. Lou (2), K. Jinno (2) (1) Section of Toxicology and Bioanalytics, Department of Environmental Biology, Koszalin
University of Technology, Śniadeckich 2, 75-453 Koszalin; Poland; (2) School of Materials Science, Toyohashi University of Technology, Toyohashi 441-8580, Japan
PW097 INTERACTION OF NATIVE α-CYCLODEXTRIN, β-CYCLODEXTRIN AND γ-CYCLODEXTRIN AND THEIR HYDROXYPROPYL DERIVATIVES WITH SELECTED ORGANIC LOW MOLECULAR MASS COMPOUNDS AT ELEVATED AND SUBAMBIENT TEMPERATURE UNDER RP-HPLC CONDITIONS
P. K. Zarzycki *(1), H. Ohta (2), Y. Saito (2), K. Jinno (2) (1) Section of Toxicology and Bioanalytics, Department of Environmental Biology, Koszalin
University of Technology, Śniadeckich 2, 75-453 Koszalin; Poland; (2)School of Materials Science, Toyohashi University of Technology, Toyohashi 441-8580, Japan
PW098 THE EFFECT OF NaOH ELUENT CONCENTRATION ON THE SEPARATION OF
MONOSACCHARIDES WITH AN ANION-EXCHANGE STATIONARY PHASE PREPARED FROM POLYSTYRENE-BASED RESIN AND A DIAMINE
Takako Masuda-Hanada *, Kei-ichi Kitahara, Sadao Arai Department of Chemistry, Tokyo Medical University, Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan PW099 RETENTION CONTROLLING TECHNIQUE FOR CATIONS BY CHANGING MOBILE
PHASE COMPOSITION IN NON-SUPPRESSED ION CHROMATOGRAPHY Hidetoshi Terada *, Atsushi Ieuji, Jun Nagata, Hirohisa Mikami Analytical Applications Department, Shimadzu Corporation, Nishinokyo, Nakagyo-ku, Kyoto,
604-8511, Japan PW100 MICRO THIN-LAYER CHROMATOGRAPHY SHOWS POTENTIAL TO KICK ITS
COLUMN COUNTERPART OUT OF THE LIME LIGHT P.K. Zarzycki * Section of Toxicology and Bioanalytics, Department of Environmental Biology, Koszalin University of
Technology, Śniadeckich 2, 75-453 Koszalin; Poland PW101 CHROMATOGRAPHIC BEHAVIOR OF C60 AND C70 FULLERENES AT SUBAMBIENT
TEMPERATURE WITH N-ALKANES MOBILE PHASES H. Ohta (1), Y. Saito (1), K. Jinno (1), P.K. Zarzycki (2) (1) School of Materials Science, Toyohashi University of Technology, Toyohashi 441-8580, Japan; (2)
Section of Toxicology and Bioanalytics, Department of Environmental Biology, Koszalin University of Technology, Śniadeckich 2, 75-453 Koszalin; Poland
PW102 FAST SEPARATION AND QUANTIFICATION OF C60 AND C70 FULLERENES USING
THERMOSTATED MICRO THIN-LAYER CHROMATOGRAPHY P. K. Zarzycki *(1), H. Ohta (2), F. B. Harasimiuk (1), K. Jinno (2) (1) Section of Toxicology and Bioanalytics, Department of Environmental Biology, Koszalin
University of Technology, Śniadeckich 2, 75-453 Koszalin; Poland; (2) School of Materials Science, Toyohashi University of Technology, Toyohashi 441-8580, Japan
PW103 STUDY ON THE RETENTION EQUATION IN HYDROPHILIC INTERACTION LIQUID
CHROMATOGRAPHY Gaowa Jin, Xingya Xue, Xinmiao Liang * Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China PW104 MICROWAVE IRRADIATION USED TO IMMOBILIZE STATIONARY PHASE FOR
CHROMATOGRAPHIC AND ELECTROCHROMATOGRAPHIC SEPARATION Yu Ping Zhang *(1), Na Chen (2), Pu Hong Deng (1), Wei Li (2), Ling Bo Qu (2) (1) Henan Institute of Science and Technology, Xinxiang 453003, China; (2) Department of
Chemistry, Zheng Zhou University, Henan 450052, China PW105 DEVELOPMENT OF ON-LINE DETECTION FOR MAGNETIC SEPARATION USING
QCM Hweiyan Tsai *(1), Yi Lin (1), Y.C. Ho (1), C. Bor Fuh (2) (1) Department of Applied Chemistry, Chung Shan Medical University, Taichung 402, Taiwan; (2)
Department of Applied Chemistry, National Chi Nan University, Puli, Nantou 545, Taiwan PW106 NOVEL TECHNIQUES OF BORONATE AFFINITY CHROMATOGRAPHY FOR
CAPTURE AND SEPARATION OF CIS-DIOL CONTAINING BIOMOLECULES: CAN WE TEACH AN OLD DOG NEW TRICKS?
Zhen Liu * Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering,
Nanjing University, Nanjing 210093, China PW107 DEVELOPMENT OF A VALIDATED REVERSED STATIONARY PHASE OPTIMIZED
HPLC METHOD FOR DETERMINATION OF RELATED SUBSTANCES IN NEW DRUG CANDIDATES
Dong-ying Chen *(1), Jing Lu (1), Ming Ji (1), Karl-Siegfried Boos (2), Rosa Morello (2) (1) Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zuchongzhi Rd.,
Zhangjiang High Technology Park, 201203, Shanghai, China; (2) Laboratory of BioSeparation, Institute of Clinical Chemistry, Medical Center of the University of Munich, Marchioninistr. 15, 81377 Munich, Germany
PW108 PHOSPHOLIPID CAPPING-IMPROVED BIOCOMPATIBILITY AND
WATER-SUSPENSIBILTY OF MESOPOROUS SILICA NANOPARTICLES Li-Sheng Wang *, Ja-an Annie Ho BioAnalytical Laboratory, Department of Chemistry, National Tsing Hua University, Hsin-chu, 30013
Taiwan PW109 POLY(N-VINYLCARBAZOLE)-GRAFTED SILICA AND POLY(2-N-CARBAZOLYLETHYL
ACRYLATE)-GRAFTED SILICA AS NEW POLYMERIC ADSORBENTS FOR BOTH NORMAL-PHASE AND REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
Usha Ghimire Gautam *, Mani P. Gautam, Makoto Takafuji, Hirotaka Ihara Department of Applied Chemistry and Biochemistry, Kumamoto University, 2-39-1 Kurokami,
Kumamoto 860-8555, Japan PW110 SYNTHESIS OF PERFUSIVE SILICA MICROSPHERES AND ITS APPLICATION IN FAST
SEPARATION Zhi-Guo Shi *, Yu-Qi Feng Department of Chemistry, Wuhan University, Wuhan 430072 PR China PW111 COMPETITIVE IMMUNOASSAY OF MAGNETIC SEPARATION FOR PROTEIN
DETECTION C. Bor Fuh *(1), H. Y. Tsai (2), S.J. Jian(1) , S. T. Huang (1) (1) Department of Applied Chemistry, National Chi Nan University, Puli, Nantou,Taiwan; (2) School
of Applied Chemistry, Chung Shan Medical University, Taichung, Taiwan PW112 SOL-GEL SYNTHESIS OF MACORO-MESOPOROUS TITANIA MONOLITHS AND
THEIR APPLICATIONS TO HPLC SEPARATION MEDIA FOR PHOSPHORUS-CONTAINING COMPOUNDS
Koji Fujita *(1), Junko Konishi (1), Kazuki Nakanishi (2), Kei Morisato (3), Shota Miyazaki (3), Masayoshi Ohira (3)
(1) Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan; (2) Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan; (3) GL Sciences Inc., 237-2 Sayamagahara, Iruma, Saitama, 358-0032, Japan
PW113 STRUCTURE AND PERFORMANCE OF SILICA-BASED MONOLITHIC COLUMNS K. Cabrera, K. Kuehne, A. Piper, P. Knoell, Y. Faupel, K. Kreher, S. Altmaier Merck KGaA, Darmstadt, Germany PW114 EFFECT OF THE DIRECTION OF ESTER LINKAGE ON MOLECULAR SHAPE
SELECTIVITY THROUGH MULTIPLE CARBONYL π-INTERACTION WITH OCTADECYL CHAIN-BRANCHED POLYMERS AS ORGANIC PHASES IN RP-HPLC
Ashequl A. Rana *, Makoto Takafuji, Hirotaka Ihara Department of Applied Chemistry & Biochemistry, Graduate School of Science & Technology,
Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan PW115 TEMPERATURE-RESPONSIVE CHROMATOGRAPHY USING FUNCTIONAL
POLYMERS OF D- AND L-PROLINE DERIVATIVES Hiroki Kobayashi *(1), Makoto Ishikawa (1), Chikako Sakamoto (1), Tadashi Nishio (1), Hideko
Kanazawa (1), Akihiko Kikuchi (2), Kenichi Nagase (3), Teruo Okano (3) (1) Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato-ku, Tokyo 105-8512, Japan; (2)
Department of Materials Science and Technology, Tokyo University of Science, 2641Yamazaki, Noda, Chiba 278-8510, Japan; (3) Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1Kawadacho, Shinjuku-ku, Tokyo 162-8666, Japan
PW116 COMPLEXATION BEHAVIOR OF MONO- AND DISACCHARIDES WITH THE
VINYLBENZENEBORONIC ACID - DIVINYLBENZENE COPOLYME RESINS PACKED IN A HPLC COLUMN
Kei-ichi Kitahara *(1), Yuji Noguchi (2), Satoshi Itoh (2), Nobunao Chiba (2), Tasuku Tohyama (2), Kunio Nagashima (2), Takako Hanada (1), Isao Yoshihama (3), Sadao Arai (1)
(1) Department of Chemistry, Tokyo Medical University, Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan; (2) Department of Applied Chemistry, Kogakuin University, Nishishinjuku, Shinjuku-ku, Tokyo 163-8677, Japan; (3) Electron Microscopy Laboratory, Tokyo Medical University, Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan
PW117 AQUEOUS CHROMATOGRAPHIC SYSTEM FOR QUANTIFICATION OF
PHARMACEUTICAL COMPOUNDS USING TEMPERATURE-RESPONSIVE POLYMER MODIFIED STATIONARY PHASE
Tadashi Nishio *(1), Hideko Kanazawa (1), Teruo Okano (2), Takako Miyabe (3) (1) Department of Physical Pharmaceutical Chemistry, Keio University, 1-5-30, Shibakoen,
Minato-ku, Tokyo, 105-8512, Japan; (2) Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1, Kawadacho, Shinjuku-ku, Tokyo, 162-8666, Japan; (3) Primate Research Institute, Kyoto University, Inuyama, Aichi, 484-8506, Japan
PW118 HYDROPHOBIC INTERACTION MECHANISM IN WEAK ANION EXCHANGE
CHROMATOGRAPHY COLUMN Peng Liu, Haiya Yang, Xindu Geng * Institute of Modern Separation Science, Key Laboratory of Separation Science in Shaanxi Province,
Department of Chemistry, Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Northwest University, Xi’an 710069, China
PW119 HIGH MECHANICAL STABILITY REVERSED PHASE GEL WITH ACID-RESISTANT
FOR PURIFICATION OF PEPTIDES AND PROTEINS Noriko Shoji *, Yosuke Matsushita, Kazumichi Takahashi, Tomohiro Kinoshita, Kiyoshi Morishita,
Masakatsu Omote, Naohiro Kuriyama YMC Co., Ltd., Kokufudai, Komatsu 923-8557, Japan PW120 STUDY OF SECONDARY INTERACTION BASED ON RESIDUAL SILANOL GROUPS
FOR REVERSED-PHASE LIQUID CHROMATOGRAPHY Norikazu Nagae *, Koji Yamamoto, Chiaki Kadota ChromaNIk Technologies Inc., 6-3-1 Namiyoke, Minato-ku, Osaka 552-0001, Japan PW121 THE EFFECT OF CHROMATOGRAPHIC CONDITIONS ON THE RETENTION
BEHAVIOR OF PURINE DERIVATIVES ON TITANIA Masatake Ozawa *(1, 2), Kazue Tani (2), Masaki Tachibana (2), Hitoshi Koizumi (2), Nobutoshi
Kiba (2) (1) Environmental Business Department, Koizumi Co., Ltd., Annex Bld. 6F, 4-32-9, Ogikubo
Suginami-ku, Tokyo 167-0051, Japan; (2) Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 4-37 Takeda-4, Kofu 400-8510, Japan
PW122 NEW STATIONARY PHASE FOR SILVER ION HIGH-PERFORMANCE LIQUID
CHROMATOGRAPHY: COLUMN STABILITY, AND SEPARATION OF CIS-TRANS AND POSITIONAL ISOMERS
Nobuyuki Ohtaki *, Kazunori Yoshino Central Research Laboratory, Kanto Chemical CO.,INC., 1-7-1, Inari, Soka-shi, Saitama 340-0003,
Japan PW123 SUB-2μM POLYMER-COATED C18 STATIONARY PHASE FOR FAST SEPARATION OF
BASIC COMPOUNDS Yoshihisa Hiroe *, Taketoshi Kanda Shiseido Research Center, 2-2-1 Hayabuchi, Tsuzuki, Yokohama, Japan 224-8558 PW124 SOME CONSIDERATIONS TO ACHIEVE HIGH QUALITY ULTRA HIGH RESOLUTION
ANALYSIS USING ULTRA FAST LIQUID CHROMATOGRAPH Kenichi Yasunaga *, Masami Tomita, Yusuke Osaka, Yoshiaki Maeda, Yoshiaki Aso, Yoshihiro
Hayakawa, Shuzo Maruyama Shimadzu Corporation, Nakagyo-ku, Kyoyo 604-8511, Japan PW125 A NOVEL ON-LINE ULTRA FAST LIQUID CHROMATOGRAPHY SYSTEM FOR
PHARMACEUTICAL PROCESS ANALYTICAL TECHNOLOGY Daisuke Nakayama *, Yousuke Iwata, Shuzo Maruyama Shimadzu Corporation, Kyoto, Japan PW126 PREPARATION OF MONOLITHIC SILICA CAPILLARY COLUMNS FOR CHIRAL
SEPARATION BY ON-COLUMN POLYMERIZATION, AND THE EVALUATION OF THEIR SEPARATION EFFICIENCY
Tohru Ikegami *, Masashi Matsumoto, Hideaki Ishii, Yukiko Miyaji, Nobuo Tanaka Department of Biomolecular Engineering, Graduate School of Science and Technology, Kyoto
Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan
Poster Session 2 (PT), Thursday, December 4, 12:30–14:30 (PT posters duty: 13:30–14:30)
Instrumentation / Detection / Multi-Dimensional Operation
PT131 “STRAIGHT-TO-COLUMN” STRUCTURE, THE NOVEL MECHANISM DESIGNED FOR
LC AUTOSAMPLER WITH ULTIMATE SEPARATION EFFICIENCY AND ANTI-CARRYOVER PERFORMANCE
Osamu Shirota *, Kazuhiko Mibayashi, Yasutaka Naruse, Masashi Mita Shiseido Research Center, 2-2-1 Hayabuchi Tsuzuki-ku Yokohama Japan 224-8558 PT132 HIGH PERFORMANCE CHROMATOGRAPHY COMBINED WITH SOLUTION X-RAY
SCATTERING OPTICS Yasushi Watanabe *(1), Yoji Inoko (2) (1) National Food Research Institute, Kannondai 2-1-12, Tsukuba, Ibaraki 305-8642, Japan; (2)
Graduate School of Engineering Science, Osaka University, Machikaneyama 1-12, Toyonaka, Osaka 565-0871, Japan
PT133 A NOVEL LASER-INDUCED FLUORESCENCE DETECTOR WITH UV AXIAL-BEAM
EXCITATION Ayako Tamura *, Masakazu Izako, Tsuyoshi Yamada, Xiaojing Zhou Department of Research and Development, GL Sciences, 237-2 Sayamagahara, Iruma, Saitama
358-0032, Japan PT134 NEW HIGH-THROUGHPUT LIQUID CHROMATOGRAPHY SYSTEM WITH
ATTRIBUTES OF INCREASED RESOLUTION AND SENSITIVITY Kyo Miyaki *, Hideyuki Uzu, Yoshihiko Takano, Xiaojing Zhou Department of Research and Development, GL Sciences, 237-2, Sayamagahara, Iruma, Saitama,
358-0032 Japan PT135 AGILENT 1200 SERIES LC METHOD DEVELOPMENT SOLUTION – AUTOMATED
METHOD DEVELOPMENT WITH UP TO EIGHT COLUMNS Fuminori Nishimoto *(1), Hiroki Kumagai (1), Angelika Gratzfeld-Huesgen (2), Michael Frank (2) (1) Agilent Technologies Inc., Hachioji 192-8510, Japan; (2) Agilent Technologies GmbH, D-76337
Waldbronn, Germany PT136 DEVELOPMENT OF THE NEW NANOFLOW HPLC SYSTEM Yusuke Osaka *, Yosuke Iwata, Takaei Kitagawa, Shinichiro Kobayashi, Shuzo Maruyama Shimadzu Corporation, Nakagyo-ku, Kyoto 604-8511, Japan PT137 DEVELOPMENT OF FLUOROUS TAG-BOUND FLUORESCENCE DERIVATIZATION
REAGENT, F-TRAP PYRENE, FOR REAGENT'S PEAK-FREE HPLC ANALYSIS OF BIOLOGICAL AMINES
Kenichiro Todoroki *, Hidemichi Etoh, Hideyuki Yoshida, Hitoshi Nohta, Masatoshi Yamaguchi Faculty of Pharmaceutical Sciences, Fukuoka University, Nanakuma, Johnan-ku, Fukuoka 814-0180,
Japan PT138 LIQUID CHROMATOGRAPHIC DETERMINATION OF LIPOIC ACID-RELATED
COMPOUNDS BASED ON INTRAMOLECULAR EXCIMER-FORMING DERIVATIZATION AND FLUORESCENCE DETECTION
Hideyuki Yoshida *, Takashi Inoue, Hiroki Umemura, Maki Sudo, Kenichiro Todoroki, Hitoshi Nohta, Masatoshi Yamaguchi Faculty of Pharmaceutical Sciences, Fukuoka University, Nanakuma, Johnan-ku, Fukuoka 814-0180,
Japan PT139 INTEGRATED MICRO-OPTICAL DETECTION UNIT FOR FLUORESCENCE AND
ABSORBANCE MEASUREMENTS IN CAPILLARIES FOR CHROMATOGRAPHIC APPLICATIONS
Sara Van Overmeire *(1), Heidi Ottevaere (1), Gert Desmet (2), Hugo Thienpont (1) (1) Department of Applied Physics and Photonics; (2) Department of Chemical Engineering, FirW-CHIS, Vrije Universiteit Brussel, B-1050 Brussels, Belgium
PT140 AN ATTOMOLE DNA ELECTROCHEMICAL SENSOR FOR THE DETECTION OF
ESCHERICHIA COLI O157 Wei-Ching Liao *, Ja-an Annie Ho
BioAnalytical Laboratory, Department of Chemistry, National Tsing Hua University, Hsin-chu 30013 Taiwan
PT141 DIAGNOSTIC DETECTION OF HUMAN LUNG CANCER MARKER USING GOLD
NANOPARTICLE-BASED ELECTROCHEMICAL IMMUNOSENSOR Heng-Chia Chang *, Ja-an Annie Ho
BioAnalytical Laboratory, Department of Chemistry, National Tsing Hua University, Hsin-chu 30013 Taiwan
PT142 CARBON NANOPARTICLE-BASED IMMUNOELECTROCHEMICAL DETECTION FOR
CARCINOEMBRYONIC ANTIGEN WITH CdS NANOCRYSTAL Yeh-Chun Lin *, Li-Sheng Wang, Ja-an Annie Ho
BioAnalytical Chemistry Laboratory, Department of Chemistry, National Tsing Hua University, Hsinchu, 30013 Taiwan
PT143 SIMULTANEOUS MICROANALYSIS OF NEUTRAL REDUCING-SACCHARIDES AND
URONIC ACIDS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH POSTCOLUMN FLUORESCENCE DERIVATIZATION
Hirotaka Kakita *(1, 2), Hiroshi Kamishima (1), Kuniyo Inouye (2) (1) AIST Shikoku, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14, Hayashi-cho, Takamatsu, Kagawa 761-0395, Japan; (2) Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan
PT144 HIGHLY SENSITIVE ANALYSIS OF CARBOHYDRATES BY HYDROPHILIC
INTERACTION CHROMATOGRAPHY WITH POSTCOLUMN FLUOROMETRIC
DETECTION Kyoko Watanabe *(1), Takeshi Goto (1), Hiroko Yamamoto (2), Hirohisa Mikami (1)
(1) Analytical Applications Department, Shimadzu Corporation, Nishiniokyo, Nakagyo-ku, Kyoto 605-8511, Japan; (2) Shimadzu Analytical & Measuring Center, Inc., Horiyamashita, Hatano, Kanagawa 259-1304, Japan
PT145 SIMPLE AND RAPID DETERMINATION OF THIOL COMPOUNDS BY HPLC WITH
1,3,5,7-TETRAMETHYL-8-PHENYL-(2-MALEIMIDE)DIFLUOROBORADIAZA-S- INDACENE Xiao-Feng Guo, Yue-Hong Guo, Hong Wang, Hua-Shan Zhang *
Department of Chemistry, Wuhan University, Wuhan 430072, China PT146 SIMULTANEOUS DETECTION OF DRUGS IN BLOOD BY COLUMN-SWITCHING HPLC
METHOD USING A COMBINATION OF BSA-COATED ODS PRECOLUMN AND NOVEL NH2 TYPE HILIC COLUMN
Yasutoshi Kawai *, Hiroyuki Yamasaki, Michiko Sakata, Hiroyuki Moriyama Separation Center, TOSOH Corporation, Kaisei-cho 4560, Shu-nan, Yamaguchi 746-8501, Japan
PT147 EVALUATION OF COLUMNS FOR BETTER SEPARATION OF MULTIDIMENSIONAL
ION EXCHANGE CHROMATOGRAPHY Jianfeng Li *, Robert A. Shellie, Paul R. Haddad
Australian Centre for Research on Separation Science (ACROSS), School of Chemistry, University of Tasmania, Private Bag 75, Hobart 7001 Australia
PT148 ESTABLISHMENT AND APPLICATION OF COMPREHENSIVE TWO DIMENSIONAL
NPLC×RPLC Yuanlong Wei *(1), Tao Lan (2), Tao Tang (3), Weibing Zhang (1), Yiping Du (1), Tong Li (3),
Fengyun Wang (2) (1) School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, China; (2) Industrial Chemical Institute, Nanjing University of Science and Technology, Nanjing, 210094, China; (3) Dalian Elite Analytical Instruments, Co. Ltd., Dalian, 116023, China
PT149 EMPLOYMENT OF COMPREHENSIVE TWO-DIMENSIONAL LIQUID
CHROMATOGRAPHY FOR QUANTIFICATION OF POLYPHENOLS IN RED WINES Francesco Cacciola (1), Miguel Herrero (1), Paola Donato (1), Paola Dugo (2), Luigi Mondello *(1)
(1) Dipartimento Farmaco-chimico, Università di Messina, Viale Annunziata, 98168 Messina, Italy; (2) Dipartimento di Scienze degli Alimenti e dell’Ambiente, Università di Messina, Salita Sperone, 98166 Messina, Italy
PT150 TWO-DIMENSIONAL LC-GC WITH POLYMER-COATED FIBROUS MATERIALS AS
THE INTERFACING MEDIUM Akira Abe *, Ikuo Ueta, Yoshihiro Saito, Kiyokatsu Jinno
School of Materials Science, Toyohashi University of Technology, Toyohashi 441-8580, Japan PT151 DEVELOPMENT OF MULTIPLY HYPHENATED COMPREHENSIVE ANALYSIS SYSTEM
FOR OMICS RESEARCH Tomonari Umemura *, Hiroharu Kobayashi, Yuka Takasaki
Division of Nano-materials Science, EcoTopia Science Institute, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan
PT152 TWO-DIMENSIONAL CAPILLARY HPLC IN A REVERSED-PHASE MODE USING TWO
C18 MONOLITHIC SILICA COLUMNS MODIFIED WITH OCTADECYL MOIETY AND POLY(OCTADECYL METHACRYLATE)
Thumnoon Nhujak *(1), Wasura Soonthorntantikul (1), Tohru Ikegami (2), Nobuo Tanaka (2) (1) Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; (2) Department of Biomolecular Engineering, Graduate School of Science and Technology, Kyoto Institute of Technology, Kyoto, 6068585, Japan
PT153 SIMULTANEOUS DETERMINATION OF SMALL AMOUNTS OF D-SERINE AND D-ALANINE IN MOUSE TISSUES AND PHYSIOLOGICAL FLUIDS USING TWO-DIMENSIONAL MICRO-HPLC
Yurika Miyoshi *(1), Kenji Hamase (1), Yosuke Tojo (1, 2), Masashi Mita (2), Tsuneaki Kaneko (2), Wolfgang Lindner (3), Kiyoshi Zaitsu (1) (1) Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; (2) Shiseido Co., Ltd., 1-1-16 Higashi-shimbashi, Minato-ku, Tokyo 105-0021, Japan; (3) Institute of Analytical Chemistry and Food Chemistry, University of Vienna, Waehringerstrasse 38, A-1090 Vienna, Austria
LC Applications PT154 EXPLOSIVES ANALYSIS BY ULTRA-FAST LIQUID CHROMATOGRAPHY William Hedgepeth, Masatoshi Takahashi *, James Mott
Shimadzu Scientific Instruments, 7102 Riverwood Drive, Columbia MD 21046 USA PT155 STUDY OF MICROWAVE-ENHANCED CATALYTIC DEGRADATION OF PHENOL OVER
NICKEL OXIDES UNDER LOW TEMPERATURE MONITORED BY HPLC METHOD Zih-Yu Shih (1), Teh-Long Lai (1), Chen-Bin Wang (2), Youn-Yuen Shu *(1)
(1) Environmental Analysis and Microwave-enhanced Technology Laboratory, Department of Chemistry, National Kaohsiung Normal University, Kaohsiung 802, Taiwan; (2) Department of Applied Chemistry and Materials Science, National Defense University, Taoyuan 335, Taiwan
PT156 ANALYSIS OF SYNTHETIC POLYMERS USING SEC– MALDI-TOFMS Hiroki Nakajima *, Shin-ichirou Kawabata
MS Business Unit / Application Development Center Tokyo, Analytical and Measurement Instruments Division, Shimadzu Corporation, 1 Nishinokyo-Kuwabaracho, Nakagyo-ku, Kyoto 604-8511, Japan
PT157 SIMULTANEOUS ANALYSIS OF PLASMA THIOLS BY HPLC WITH FLUORESCENCE
DETECTION USING A NEW PROBE: 1,3,5,7-TETRAMETHYL-8-PHENYL-(4- IODOACETAMIDO)DIFLUOROBORADIAZA-S-INDACENE
Xiao-Feng Guo, Yue-Hong Guo, Hua-Shan Zhang, Hong Wang * Department of Chemistry, Wuhan University, Wuhan 430072, China
PT158 FAST SCREENING OF FREE STEROIDS AND RELATED LOW MOLECULAR MASS
COMPOUNDS FRACTION IN THE ENVIRONMENTAL SAMPLES DERIVED FROM TREATED AND UNTREATED SEWAGE WATER
E. Włodarczyk *, M. Baran, P.K. Zarzycki Section of Toxicology and Bioanalytics, Department of Environmental Biology, Koszalin University of Technology, Śniadeckich 2, 75-453 Koszalin; Poland
PT159 DETERMINATION OF EDCs LIKE COMPOUNDS USING TEMPERATURE DEPENDENT
INCLUSION CHROMATOGRAPHY P. K. Zarzycki *, E. Włodarczyk, M. Baran
Section of Toxicology and Bioanalytics, Department of Environmental Biology, Koszalin University of Technology, Śniadeckich 2, 75-453 Koszalin; Poland
PT160 DETERMINATION OF URINARY CARCINOGENIC PAHS MONOHYDROXY
METABOLITES BY LIQUID CHROMATOGRAPHY–TANDEM MASS SPECTROMETRY Ting Ruan (1), Jianbo Shi (1), Yongguang Yin (1), Fusheng Wei (1, 2), Guibin Jiang *(1)
(1) State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 100085, Beijing; (2) China National Environmental Monitoring Center, Beijing 100029, China
PT161 THE DETECTION OF CHLORAMPHENICOL DRUGS RESIDUES IN PENAEUS
VANNAMEI BY HPLC AND LC-MS Xie Wan-Cui *, Yang Xi-Hong, Zhang Chaohua, Wu Xiao-Ping, Wang Weimin
School of Food Science and Technology, Guangdong Ocean University, Guangdong, Zhanjiang,
524025, China PT162 SEPARATION AND QUANTIFICATION OF SELECTED ESTROGENS IN WATER AND
BIOLOGICAL MATRIX BY HPLC Zuzana Bosáková *(1), Marie Honetschlägerová - Vadinská (1), Eva Tesařová (2), Květa Kalíková (2), Lucie Loukotková (1), Jitka Vašinová (3), Michaela Jursová (3), Kateřina Dvořáková-Hortová (3)
(1) Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Albertov 2030, 128 40 Prague 2, Czech Republic; (2) Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University in Prague, Albertov 2030, 128 40 Prague 2, Czech Republic; (3) Department of Developmental Cell Biology, Faculty of Science, Charles University in Prague, Viničná 7, 128 44 Prague 2, Czech Republic
PT163 HYDROPHILIC INTERACTION CHROMATOGRAPHY-MASS SPECTROMETRY IN THE
ANALYSIS OF CARBOXYLIC ACIDS IN ATMOSPHERIC AEROSOL PARTICLES Joonas Nurmi *, Kari Hartonen, Marja-Liisa Riekkola
Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, P.O. BOX 55 FI-00014 University of Helsinki, Finland
PT164 EFFECT OF GAMMA-RAY IRRADIATION ON DEGRADATION OF
DI(2-ETHYLHEXYL)PHTHALATE IN POLYVINYL CHLORIDE SHEET Rie Ito *(1), Naoko Miura (1), Masaru Ushiro (1), Jun-ichi Ogino (2), Manabu Oishi (2), Yusuke
Iwasaki (1), Koichi Saito (1), Hiroyuki Nakazawa (1) (1) Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan; (2) Toray Research Center, Inc., 3-3-7 Sonoyama, Otsu, Shiga 520-8567, Japan
PT165 ANALYSIS OF HALOGENATION DISINFECTION SUB-PRODUCTS IN TAP WATER BY
LC-MS/MS Seiji Ito *, Fumiya Nakata, Yuji Sawada
Tosoh Corporation, 2743-1 Hayakawa, Ayase, Kanagawa 252-1123, Japan PT166 DETERMINATION OF NITRATED AND OXYGENATED POLYCYCLIC AROMATIC
HYDROCARBONS IN AMBIENT AIR SAMPLE BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD
Tzu-Ling Wong *, Youn-Yuen Shu Environmental Analysis and Microwave-enhanced Technology Laboratory, Department of Chemistry, National Kaohsiung Normal University, Kaohsiung 802, Taiwan
PT167 ANALYSIS OF HALOACETIC ACIDS IN DRINKING WATER BY NON-SUPPRESSED ION
CHROMATOGRAPHY-MASS SPECTROMETRY Jun Nagata *, Akio Tominaga, Naoki Hamada, Hirohisa Mikami
Analytical Applications Department, Shimadzu Corporation, Horiyamashita, Hadano, Kanagawa 259-1304, Japan
PT168 A NEW ANALYTICAL METHOD FOR THE TOTAL ANTIOXIDANT ASSAY USING
RP-HPLC WITH FLUORESCENCE DETECTION AND ITS APPLICATION FOR ANTIOXIDATIVE PROPERTIES OF SOME HERBAL AND FOOD PRODUCTS
Pawel Piszcz, Agnieszka Beta, Iwona Kiersztyn, Bronislaw K. Glod * Department of Analytical Chemistry, University of Podlasie, ul. 3 Maja 54, 08-110 Siedlce, Poland
PT169 EFFECT OF ENDOGENOUS GLYCOSIDASE ON STABILITY OF STEROIDAL SAPONINS
IN YAM DURING DRYING PROCESSES DETERMINED BY HPLC Deng-Jye Yang *(1), Ting-Jang Lu (2), Lucy Sun Hwang (2)
(1) School of Diet and Restaurant Management, Chung Shan Medical University, 110, Jianguo N. Rd. Sec. 1, Taichung 402, Taiwan; (2) Graduate Institute of Food Science and Technology, National Taiwan University, 1, Roosevelt Rd., Sec. 4, Taipei, Taiwan 106
PT170 DETERMINATION OF GANGLIOSIDES IN MILK AND INFANT FORMULAS BY LC/MS
METHODS
Yi-Bao Jin (1, 3), Hui Li (1, 3), Hong-Xia Liu *(1), Zhi-Guo Yu (3), Chun-Mei Gao (1), Zong-Wei Cai (4), Yu-Yang Jiang (1, 2) (1) The Key Laboratory of Chemical Biology, Guangdong Province, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055,China; (2) Tsinghua University, school of medicine, Beijing, 100084,China; (3) Shenyang Pharmaceutical University, Shenyang, 110016,China; (4) Department of Chemistry, Hong Kong Baptist University, Kowloon, Hong Kong SAR, China
PT171 DETERMINATION OF GAMMA-AMINOBUTYRIC ACID IN GABA DIETARY
SUPPLEMENT BY NOVEL DIRECT AQUEOUS-PHASE DERIVATIZATION AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-DIODE ARRAY DETECTOR
Kornthip Boonmee *(1, 2), Nitirat Chimnoi (3), Chutiphon Chongkaewatthana (2), Malai Boonyaratanakornkit (2), Siriwit Buajarern (1) (1) Department of Chemistry, Faculty of Science and Technology, Thammasat University, Pathumthani 12121, Thailand; (2) Institute of Food Research and Product Development, Kasetsart University, Bangkok 10900, Thailand; (3) Chulabhorn Research Institute, Bangkok 10210, Thailand
PT172 INVESTIGATION OF THE EFFECT OF TEMPERATURE ON THE FORMATION OF
ACRYLAMIDE IN HEAT-PROCESSED MALT BY LC/MS/MS Zhihong Shi *(1), Yanmei Zhang (1), Yanzhong Cao (2), Hongyi Zhang (1)
(1) College of Chemistry and Environmental Science, Hebei University, Baoding, 071002, China; (2) Qinhuangdao Entry-Exit Inspection and Quarantine Bureau, 39 Haibin Rd, Qinhuangdao, 066002, China
PT173 HPLC ANALYSIS OF TEA CATECHINS USING CYCLODEXTRIN-BONDED SILICA
COLUMN Hisako Okumura *(1), Yutaka Ikeda (1), Masaki Ichitani (2), Takanobu Takihara (2), Ko-Ki
Kunimoto (1) (1) Graduate School of Natural Science & Technology, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa 920-1192, Japan; (2) Central Research Institute, ITO EN, LTD., 21 Mekami, Makinohara, Shizuoka 421-0516, Japan
PT174 SIMULTANEOUS ANALYSIS OF ARTIFICIAL SWEETNESS USING CYANOPROPYL
BONDED SILICA GEL COLUMN AND EVAPORATIVE LIGHT SCATTERING DETECTOR
Hiroki Kumagai *, Akio Kori LC Products Sales, Life Science and Chemical Analysis Division, Agilent Technologies Inc., Hachioji 192-0033, Japan
PT175 QUANTITATIVE ANALYSIS OF ARBUTIN AND HYDROQUINONE IN BEARBERRY
EXTRACTS BY REVERSED- PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH ON-LINE PRECONCENTRATION AND UV-VISIBLE DETECTION
Yun-Yun Sun *(1), Chin-Hsing Chen (2) (1)Department of Cosmetic Applications and Management, Tung Fang Institute of Technology, Kaohsiung,Taiwan, Repubilc of China; (2)Institute of Chemical Engineering, Chinese Culture University, Taipei, Taiwan, Republic of China
PT176 OPTIMIZATION OF AN RP-HPLC METHOD FOR THE IDENTIFICATION AND
QUANTIFICATION OF OXYGEN HETEROCYCLIC COMPONENTS IN CITRUS OILS Maria Cambria (1), Marina Russo (1), Paola Dugo (2), Giovanni Dugo (2), Luigi Mondello *(1)
(1) Dipartimento Farmaco-chimico, Università di Messina, Viale Annunziata, 98168 Messina, Italy; (2) Dipartimento di Scienze degli Alimenti e dell’Ambiente, Università di Messina, Salita Sperone, 98166 Messina, Italy
PT177 CHARACTERIZATION OF PHOSPHOLIPIDS MOLECULAR SPECIES IN MILK BY
USING A HYBRID IT-TOF-MS ANALYZER Miguel Herrero (1), Paola Donato (1), Paola Dugo (2), Luigi Mondello *(1)
(1) Dipartimento Farmaco-chimico, Università di Messina, Viale Annunziata, 98168 Messina, Italy; (2) Dipartimento di Scienze degli Alimenti e dell’Ambiente, Università di Messina, Salita Sperone,
98166 Messina, Italy PT178 COMPREHENSIVE LIQUID CHROMATOGRAPHY–TANDEM MASS SPECTROMETRY
OF CAROTENOIDS Paola Donato (1), Daniele Giuffrida (2), Miguel Herrero (1), Francesco Cacciola (1), Paola Dugo (2),
Luigi Mondello *(1) (1) Dipartimento Farmaco-chimico, Università di Messina, Viale Annunziata, 98168 Messina, Italy; (2) Dipartimento di Scienze degli Alimenti e dell’Ambiente, Università di Messina, Salita Sperone, 98166 Messina, Italy
PT179 FRAGMENTATION STUDY OF CATECHINS IN GREEN TEA USING A SOLUTION
PHASE HYDROGEN/DEUTERIUM EXCHANGE METHOD AND LCMS-IT-TOF TANDEM MASS SPECTROMETRY
Guoqiang Liu *(1), Shizhong Chen (1), Jing Dong (2), Leren Wan (2), Yuki Hashi (2) (1) School of Pharmaceutical Science, Peking University Health Science Center, No.38 Xueyuan Road, Beijing 1000191, P.R China; (2) Department of Analytical Instruments, Shimadzu International trading (Shanghai) Co.,Limited, 14/F Life Tower No.16 Chaoyang Men Wai Street, Beijing 100020, P.R China
PT180 SCREENING ANALYSIS OF RESIDUAL PESTICIDES IN PROCESSED FOODS BY
ACCURATE MASS MEASUREMENT Kiyomi Arakawa *(1), Manami Kobayashi (1), Naoki Hamada (1), Satoshi Takatori (2), Mikiya
Kitagawa (2), Yuka Fujito (3) (1) Analytical Applications Department, SHIMADZU CORPORATION, 1, Nishinokyo-Kuwabaracho Nakagyo-ku, Kyoto 604-8511, Japan; (2) Food Chemistry Division, Osaka Prefectural Institute of Public Health, 1-3-69, Nakamichi, Higashinari-ku, Osaka 537-0025, Japan; (3) Shimadzu Analytical & Measuring Center, inc., 1, Nishinokyo-Shimoaicho Nakagyo-ku, Kyoto 604-8436, Japan
PT181 HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY – TANDEM MASS
SPECTROMETRY FOR THE MEASUREMENT OF MELAMINE IN RAT URINARY EXCRETION AND ITS TISSUE DISTRIBUTION
Yu-Tse Wu *(1), Lie-Chwen Lin *(1, 2), Chih-Min Huang (1), Chia-Chun Lin (1), Ting-Fang Chiu (2), Tung-Hu Tsai (1, 3) (1) Institute of Traditional Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan; (2) National Research Institute of Chinese Medicine, Taipei, Taiwan; (3) Department of Pediatrics, Zhongxiao Branch; Department of Education and Research, Renai Branch, Taipei City Hospital, Taipei, Taiwan
PT182 ULTRA FAST LIQUID CHROMATOGRAPHY FOR THE DETERMINATION OF
FLAVANONES IN CITRUS JUICE Makoto Ogaito *, Kyoko Watanabe, Shinichi Kawano, Keiko Yamabe, Hirohisa Mikami
Analytical Applications Department, Shimadzu Corporation, Nishinokyo, Nakagyo-ku, Kyoto 604-8511, Japan
PT183 THE HPLC STUDY OF TOCOPHEROLS HOMOLOGOUE IN THE NEEDLE OF PINUS
TABULAEFORMIS CARR Hui Duan *(1, 2)
(1) Gansu Agricultural University, 730070, Lanzhou, Gansu, China; (2) The Analysis&Research Center of Gansu Province, 730000, Lanzhou, Gansu, China
PT184 THE CHROMATOGRAPHIC INVESTIGATION OF THE PHYSICOCHEMICAL
PROPERTIES AND STABILITIES OF NEWLY SYNTHESIZED BI-1,2,4-TRIAZINES Pawel Piszcz, Agnieszka Beta, Iwona Kiersztyn, Justyna Lawecka, Andrzej Rykowski, Bronislaw K.
Glod * University of Podlasie, ul. 3 Maja 54, 08-110 Siedlce, Poland
PT185 CHARACTERIZATION AND IDENTIFICATION FOR POLYPHENOLS WITH
ANTIOXIDANT ACTIVITIES FROM ROOTS OF PARTHENOCISSUS LAETEVIRENS REHD BY HPLC/ESI-MSn
Juanjuan Chen*, Shan He, Yuanjiang Pan* Department of Chemistry, Zhejiang University, Hangzhou 310027 China
PT186 A FACILE ON-LINE RP-HPLC APPROACH TO SCREENING FOR RADICAL
SCAVENGING COMPONENTS Jindi Fu *, Cuirong Sun
Department of Chemistry, Zhejiang University, Hangzhou 310027 China PT187 HPLC DETERMINATION OF BRAIN BIOGENIC AMINES FOLLOWING TREATMENT
WITH BISPYRIDINIUM ALDOXIMES Kornelia Tekes *(1), Rudolf Laufer (2), Peter Szegi (1), Huba Kalasz (2)
(1) Department of Pharmacodynamics, Semmelweis University, Budapest, Hungary; (2) Department of Pharmacology, Semmelweis University, Budapest, Hungary
PT188 IDENTIFICATION OF MYCOBACTERIUM SPECIES USING REVERSED-PHASE HIGH
PERFORMANCE LIQUID CHROMATOGRAPHIC ANALYSIS OF MYCOLIC ACID Chen Baowen (3), Du Rong (1, 2), Guo Lei (1), Li Yang (1), Xie Jianwei* (1), Wang Guozhi (3),
Zhou Hongbing (2) (1) Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China; (2) College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China; (3) National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China
PT189 USING LIPOSOMAL FLUORESCENT BIOLABELS TO DEVELOP AN
IMMUNOAFFINITY CHROMATOGRAPHIC BIOSENSING SYSTEM FOR BIOTIN Ja-an Annie Ho *, Chi-Hsiang Hung
BioAnalytical Laboratory, Department of Chemistry, National Tsing Hua University, Hsin-chu, 30013 Taiwan
PT190 SIMULTANEOUS ELECTROSPRAY AND ATMOSPHERIC PRESSURE CHEMICAL
IONIZATION (ESI/APCI) FOR THE ANALYSIS OF HYDROPHILIC AND HYDROPHOBIC COMPOUNDS IN LIQUID CHROMATOGRAPHY / MASS SPECTROMETRY
Hirokazu Sawada *, Akio Kori LC/MS Applications Group, Life Science and Chemical Analysis Division, Agilent Technologies Inc., Hachioji 192-0033, Japan
PT191 FLUOROMETRIC DETERMINATION OF NICOTINE AND COTININE IN HUMAN
SERUM BY HPLC USING POSTCOLUMN PHOTOIRRADIATION Tatsuhiro Ota *(1), Atsushi Morikawa (1), Makoto Yasuda (1), Ken-ichi Mawatari (1), Kiyoko
Kaneko (1), Shuji Kodama (2), Masayuki Ota (1), Kazuya Nakagomi (1) (1) School of Pharmaceutical Sciences, Teikyo University, Sagamiko, Sagamihara, Kanagawa 229-0195, Japan; (2) Toyama Institute of Health, 17-1 Nakataikoyama, Imizu, Toyama 939–0363, Japan
PT192 COMPARISON OF DETECTION METHODS FOR ANALYSIS OF SACCHARIDES BY
HPLC Ayako Matsuzaki *, Masako Ishikawa, Kenichi Tanikawa, Keiko Abe, Hiroaki Nakagawa
Hitachi High-Technologies Corporation, 882 Ichige, Hitachinaka-shi, Ibaraki, 312-0033, Japan PT193 STUDY ON THE CHANGE RULE OF ENDOGENOUS PLANT HORMONES UNDER SALT
STRESS BY HPLC-MS-MS Qiaomei Lu, Tianwen Chen, Minghua Lu, Guonan Chen, Lan Zhang *
Ministry of Education Key Laboratory of Analysis and Detection for Food Safety (Fuzhou University), Department of Chemistry, Fuzhou University, Fuzhou 350002, Fujian, China; Sport Science Research Center, Fuzhou University, Fuzhou 350002, Fujian, China
PT194 ULTRA HIGH-THROUGHPUT ANALYSES OF POLAR METABOLITES WITH NEWLY
DEVELOPED 2 MICRON C18 COLUMN Noriko Shoji *, Chie Yokoyama, Takashi Sato, Masakatsu Omote, Naohiro Kuriyama
YMC Co., Ltd., Kokufudai, Komatsu 923-8557, Japan
PT195 DETERMINATION OF THE METABOLITES OF ANGIOTENSIN II BY NANOLC-MS/MS Chia-Hsien Feng (1), Chi-Yu Lu *(2)
(1) Department of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan; (2) Department of Biochemistry, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan
PT196 HIGH SPEED SEPARATION OF DABSYL-AMINO ACIDS BY ULTRA HIGH
PERFORMANCE LIQUID CHROMATOGRAPHY (UHPLC) Yasuyo Sato *, Masao Bounoshita, Keijin Iwaya, Toshihiko Miyaji, Muneo Saito
LC & SFC Application Laboratory, JASCO Corporation, 2967-5 Ishikawa-machi, Hachioji-shi, Tokyo 192-8537, Japan
PT197 DEVELOPMENT OF A NEW PROTEOMICS PLATFORM FOR DIFFERENTIAL
ANALYSIS OF NON LABELED BY LCMS COMBINED WITH A NOVEL NANO HPLC SYSTEM
Tetsuo Iida *, Yosuke Iwata, Takaei Kitagawa, Shinichi Mitsuhashi, Masumi Sakai, Takao Okado, Yusuke Osaka, Kenichi Yasunaga, Yoshihiro Hayakawa, Shuzo Maruyama LC Business Unit / Application Development Center Kyoto, Analytical and Measurement Instruments Division, Shimadzu Corporation, 1, Nishinokyo-Kuwabaracho, Nakagyo-ku, Kyoto 604-8511, Japan
PT198 INVESTIGATION OF LC OFF-LINE MALDI SYSTEM TO IDENTIFY
PHOSPHOPROTEINS USING NANOLC SYSTEM AND TIO2 METHOD Yuzo Yamazaki *, Shinichiro Kobayashi
Analytical Applications Department, Applications Development Center, Shimadzu Corporation, 1, Nishinokyo-Kuwabaracho, Nakagyo-ku, Kyoto 604-8511, Japan
PT199 METABOLIC PROFILES OF NUCLEOSIDES IN URINE AS POTENTIAL TUMOR
MARKERS DETERMINED BY GRADIENT HPLC Małgorzata Waszczuk *, Michał J. Markuszewski, Wiktoria Struck, Ewa Szymańska, Roman
Kaliszan Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdansk, Gen. J. Hallera 107, 80-416 Gdansk, Poland
PT200 DEVELOPMENT OF AN ACCURATE MASS AND RETENTION TIME (AMRT) LIBRARY
FOR IDENTIFICATION OF METABOLITES ON REVERSE PHASE LC/MS Daniel Cuthbertson (1, 2), Theodore R. Sana *(2), Steve Fischer (1)
(1) Agilent Technologies, Inc., Santa Clara, CA., USA; (2) Graduate Program in Molecular Plant Sciences, Washington State U., Pullman, WA., USA
PT201 MECHANICAL MILLING PROTOCOL FOR METABOLOME EXTRACTION AND LC-MS
ANALYSIS OF SACCHAROMYCES CEREVISIAE Stefan Jenkins (1, 2), Theodore R. Sana *(1)
(1) Agilent Technologies, Inc., Santa Clara, CA., USA; (2) Department Cell & Molecular Biology, San Francisco State U., S.F., CA., USA
PT202 DEVELOPMENT OF SCREENING SYSTEM FOR D-ASP–CONTAINING PROTEINS Kumiko Sakai-Kato *(1), Tadatoshi Kinouchi (2), Noriko Fujii (2), Kazuhiro Imai (1), Naoko
Utsunomiya-Tate (1) (1) Research Institute of Pharmaceutical Sciences, Musashino University, Shinmachi, Nishitokyo-shi, Tokyo 202-8585, Japan; (2) Research Reactor Institute, Kyoto University, Kumatori-cho, Sennan-gun, Osaka 590-0494, Japan
PT203 DETERMINATION OF ENDOGENOUS MELATONIN IN SMALL VOLUME OF
BIOLOGICAL FLUIDS USING PRECOLUMN OXIDATION AND COLUMN-SWITCHING HPLC SYSTEMDEVELOPMENT OF SCREENING SYSTEM FOR D-ASP–CONTAINING PROTEINS
Mayumi Fujimoto *(1), Rie Nagai (1), Asuka Kamizuru (1), Kenji Hamase (1), Yosuke Tojo (1, 2), Keiko Uezono (3) Akira Yoshida (4), Hiroshi Kitagawa (4), Kiyoshi Zaitsu (1)
(1) Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; (2) Shiseido Co., Ltd.,1-1-16 Higashi-shimbashi, Minato-ku, Tokyo 105-0021, Japan; (3) Institute of Health Science, Kyushu University, 6-1 Kasugakoen, Kasuga, 816-8580, Japan; (4) Koujinkai Ogawa Hospital, 205 Haragawa, Ogawa-machi 355-0317, Japan
PT204 STUDY ON THE EFFICIENT AND RAPID OXIDATIVE CONVERSION OF MELATONIN
TO A HIGHLY FLUORESCENT COMPOUND, 6-MOQMA, FOR THE SENSITIVE AND AUTOMATED HPLC DETERMINATION
Rie Nagai *(1), Mayumi Fujimoto (1), Asuka Kamizuru (1), Yosuke Tojo (1, 2), Kenji Hamase (1), Kiyoshi Zaitsu (1) (1) Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; (2) Shiseido Co., Ltd., 1-1-16 Higashi-shimbashi, Minato-ku, Tokyo 105-0021, Japan
PT205 SEQUENCE ANALYSIS OF GLYCOPEPTIDES BY ELECTROSPRAY
IONIZATION-LINEAR TRAP MASS SPECTROMETRY EMPLOYING “HOT” ELECTRON CAPTURE DISSOCIATION
Shinya Ito (1), Gary L. Glish (1), Takashi Baba (1), Jared M. Bushery (1), Masaki Watanabe (2), Hiroki Ito (2), Kuriko Yamada (2), Hiroaki Nakagawa (2), Kisaburo Deguchi *(2) (1) University of North Carolina, NC 27599, USA; (2) Hitachi High-Technologies Corp., Hitachinaka, 312-8504, Japan
PT206 PROFILING OF AMINE-CONTAINING METABOLITES USING LC ESI-MS Satoshi Yamaki *, Ken-ichiro Tanaka, Naoki Hamada, Hirohisa Mikami
Analytical Applications Department, Shimadzu Corporation, Horiyamashita, Hadano, Kanagawa 259-1304, Japan
PT207 SIMULTANEOUS DETERMINATION OF TEN MAIN COMPONENTS OF DA-CHENG-QI
DECOCTION IN DOG PLASMA BY LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY
Yu Qin (1), Liang Mao-Zhi *(1), Xiang Jin (1), Tang Wenfu (2), Qin Yong-Ping (1), Nan Feng (1) (1) Department of Clinical Pharmacology, West China Hospital, S.C.U, Chengdu 610041, P.R.China; (2) Department of integrated traditional Chinese and western medicine , West China Hospital, S.C.U, Chengdu 610041, P.R.China
PT208 PHARMACOKINETICS OF IBUPROFEN ENANTIOMERS IN HEALTHY CHINESE
HAN-VOLUNTEERS Liang Mao-zhi, Yu Qin, Xiang Jin, Qin Yong-ping, Nan Feng
Department of Clinical Pharmacology, West China Hospital,S.C.U, Chengdu, 610041, P.R.China PT209 SIMULTANEOUS QUALITATION AND QUANTIFICATION OF EIGHT BIOACTIVE
COMPOUNDS IN ZHENQIFUZHENG PREPARATION BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH DIODE ARRAY DETECTOR AND EVAPORATIVE LIGHT SCATTERING DETECTOR
Shilan Feng *, Fang Cui, Fangdi Hu, Yuqiong Wu School of pharmacy, Lanzhou University, Lanzhou, Gansu 730000, China
PT210 LC-MS/MS METHOD VALIDATION FOR QUANTIFICATION OF ACAMPROSATE
CALCIUM IN HUMAN PLASMA AND APPLICATION TO PHARMACOKINETIC STUDIES IN CHINESE VOLUNTEERS
Qin Yong-ping, Luo Ming, Liang Mao-zhi, Yu Qin *, Liao Dong-xue Department of Clinical Pharmacology, West China Hospital, Sichuan University, Chengdu 610041, China
PT211 IDENTIFICATION AND DETERMINATION OF THE MAJOR CONSTITUENTS IN
EXTRACTS OF TRADITIONAL CHINESE MEDICINAL PLANT RADIX POLYGONI MULTIFLORI BY LCMS-IT-TOF
Jing Dong *(1), Leren Wan (1), Fengyun Pan (1), Yuki Hashi (1), Shizhong Chen (2) (1) Department of Analytical Instruments, Shimadzu International trading (Shanghai) Co.,Limited,
14/F Life Tower No.16 Chaoyang Men Wai Street, Beijing 100020, P.R Chin; (2) School of Pharmaceutical Science, Peking University Health Science Center, No.38 Xueyuan Road, Beijing 1000191, P.R China
PT212 SIMULTANEOUS DETERMINATION OF DICLOFENAC AND CODEINE IN HUMAN
PLASMA BY LIQUID CHROMATOGRAPHY/TANDEM MASS SPECTROMETRY Yu Xu *, Aixiang Liu, Fei Liu, Fang Xu, Bo Jiang, Junjie Sun, Qingxiang Guo
Department of Chemistry, University of Science and Technology of China, Hefei 230026, China PT213 PICOGRAM DETERMINATION OF CAFFEINE AND SELECTED PHARMACEUTICAL
RESIDUES IN WATER BY ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY- TANDEM MASS SPECTROMETRY
Jingming Wu, Xiaoqing Qian, Junqi Yue, Zhaoguang Yang, Lifeng Zhang * Center for Advanced Water Technology, PUB Consultants, the Toh Tuck Complex, 80/82, Toh Guan Road East, Singapore 608575
PT214 DENTIFICATION OF METABOLITES OF TRICLOSAN IN RAT LIVER ENZYME AND
URINE USING IN-SOURCE FRAGMENTATION AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-ION TRAP MASS SPECTROMETRY
Jian-lin Wu, Zongwei Cai * Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR
PT215 ISOLATION AND PURIFICATION OF THREE FLAVONOIDS FROM THE HAWTHORN
LEAVES BY HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY COMBINED TOGETHER WITH ISOCRATIC PREPARATIVE REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
Honglei Xu, Yaju Pan, Tingting Zhou, Jun Wen, Guorong Fan *, Yutian Wu Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, No.325 Guohe Road, Shanghai 200433, China
PT216 PREPARATIVE SEPARATION AND PURIFICATION ISOFLAVONOIDS FROM RHIZOMA
BELAMCANDAE BY HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY Chao Feng, Xiaowei Fan, Yaju Pan, Tingting Zhou, Jun Wen, Guorong Fan *
Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, No.325 Guohe Road, Shanghai 200433, China
PT217 SEPARATION AND IDENTIFICATION OF TEN IRIDOID GLYCOSIDES IN
TRADITIONAL CHINESE MEDICINE GARDENIA JASMINOIDES ELLIS BY LIQUID CHROMATOGRAPHY-DIODE ARRAY DETECTION-ELECTROSPRAY IONIZATION MASS SPECTROMETRY
Tingting Zhou, Xiaowei Fan, Jun Wen, Guorong Fan *, Yutian Wu Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, No.325 Guohe Road, Shanghai 200433, China
PT218 IDENTIFICATION OF PLUMBAGIN AND ITS METABOLITES IN RAT BY LIQUID
CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY Yen-Ju Hsieh *(1), Lei-Chwen Lin (2), Tung-Hu Tsai (1, 3)
(1) Institute of Traditional Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan; (2) National Research Institute of Chinese Medicine, Taipei, Taiwan; (3) Department of Education and Research, Taipei City Hospital, Taipei, Taiwan
PT219 DEVELOPING THE CLASSIFICATION MODEL AND SELECTION OF POTENTIAL
QUALITY CONTROL MARKER BASED ON HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND RANDOM FORESTS: AN APPLICATION IN CODONOPSIS PILOSULA
Li Bo-Xia (1, 2), Wei Yu-Hui (1, 3), Li Shu-Yan (3), Duan Hao-Gang (1, 2), Yao Xiao-Jun (3), Wu Xin-An *(1) (1) Department of Pharmacy, the First Hospital of Lanzhou University, Lanzhou 730000, PR China; (2) College of Pharmacy, Lanzhou University, Lanzhou 730000, PR China; (3) College of Chemistry,
Lanzhou University, Lanzhou 730000, PR China PT220 THE STUDY OF STEREOSPECIFIC ANALYSIS OF CHEMICAL DRUGS AND ITS
METABOLITES BY DRUG-METABOLIZING ENZYME CYTOCHROME P450 Aya Katsuura *(1), Yusuke Iioka (1), Yoshiko Nagata (1), Shigemi Kai (2), Takashi Kojima (2),
Hideko Kanazawa (1) (1) Keio University Faculty of Pharmacy, Tokyo, Japan; (2) Kanagawa Prefectural Institute of Public Health, Kanagawa, Japan
PT221 FAST HIGH PERFORMANCE LIQUID CHROMATOGRAPHY FOR DETERMINATION
OF LAMIVUDINE, NEVIRAPINE AND ZIDOVUDINE USING 3.5 MICRON PARTICLE-PACKED COLUMNS
Lawan Sriphong *(1), Amornrut Chaidedgumjorn (1), Siriporn Kuttatharmmakul (2) (1) Department of Pharamceutical Chemistry, Faculty of Pharmacy, Silpakorn University, Sanam Chandra Palace, Nakhon Pathom 73000, Thailand; (2) Department of Quality Assurance, The Government Pharamceutical Organization, 75/1 Rama VI Road, Ratchathewi, Bangkok 10400, Thailand
PT222 THE STUDY OF THERAPEUTIC DRUG MONITORING BY ULTRA HIGH-SPEED LIQUID
CHROMATOGRAPHY Hideko Kanazawa *, Tadashi Nishio, Eri Ayano, Yoshiko Nagata
Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato-ku, Tokyo 105-8512, Japan PT223 FAST DETERMINATION OF PHENOLIC COMPOUNDS IN DIFFERENT TRADITIONAL
CHINESE MEDICINES BY A UFLC METHOD Zhaoqi Zhan *(1), Yi-Zhong Cai (2), Xing Jie (1), Fan Zhu (2), Yulia Jomono (1), Wuyang Huang (2),
Naoki Hamada (3) (1) Shimadzu (Asia Pacific) Pte Ltd, 16 Science Park Drive, #01-01 The Pasteur, Singapore Science Park, Singapore 118227; (2) School of Biological Science, The University of Hong Kong, Pokfulam Road, Hong Kong; (3) Shimadzu Corporation, Analytical Application Department, 380-1 Horiyamashita, Hadano City, Kanagawa, Japan
PT224 EVALUATION OF ANALYTICAL PERFORMANCE OF SERUM LIPOPROTEIN
PROFILES USING GEL PERMEATION COLUMNS IN DUAL ONLINE DETECTION HPLC SYSTEM
Mizuho Itoh *(1), Yukie Abe (1), Fumiko Kimura (1), Junichiro Takahashi (1), Gen Toshima (1), Hiroki Sasaki (1), Mitsuyo Okazaki (2) (1) Skylight Biotech Inc., 100-4 Sunada, Iijima, Akita 010-0911, Japan; (2) Laboratory of Chemistry, College of Liberal Arts and Sciences, Tokyo Medical and Dental University
PT225 ANALYSIS OF LIPOPROTEIN PROFILES IN CULTURE MEDIUM OF HEPATOMA
CELLS BY LIPOSEARCH®; IN VITRO SYSTEM FOR SCREENING ANTI-HYPERLIPIDEMIA DRUG
Mizuho Itoh* (1), Yuka Iwama (1), Mayumi Satho (1), Mayumi Shoji (1), Hiroki Sasaki (1), Keishi Hata (2) (1) Skylight Biotech Inc., 100-4 Sunada, Iijima, Akita 010-0911, Japan; (2) Institute for Food & Brewing, Akita Prefectural Agricultural, Forestry, and Fisheries Research Center, 4-26 Sanuki, Araya-machi, Akita 010-1623, Japan
PT226 MOLECULAR WEIGHT DETERMINATION FOR DIFFERENT SIZE OF HYALURONAN:
THE EFFECTS OF VARITY GPC ELUENT SYSTEM AND MALDI-TOFMS STUDIES Hsiao-Pin Tsai *(1), Li-Chen Wu (2), Kun-In Li (3), Chien-Chen Lai (3), Ming-Yuan Liao (1)
(1) Department of Chemistry, National Chung Hsing University, Taichung 402, Taiwan; (2) Department of Applied Chemistry, National Chi Nan University, Puli 545, Taiwan; (3) Department of Institute of Molecular Biology, National Chung Hsing University, Taichung 402, Taiwan
PT227 RESPONSE OPTIMIZATION AND NEW PREDICTIVE MODELS THROUGH
FRACTIONAL EXPERIMENTAL DESIGN AND MULTIVARIATE REGRESSION ANALYSIS IN NSAIDs SEPARATIONS
Giuseppe Carlucci *, Alessandro Marrone, Lucia Di Federico, Piera Iuliani Dipartimento di Scienze del Farmaco, Facoltà di Farmacia-Università degli Studi “G. D’Annunzio” di Chieti-Pescara, Via dei Vestini, 66100 Chieti
PT228 DIRECT LC/MS ANALYSIS OF BASIC DRUGS IN PLASMA BY ON-LINE
PRETREATMENT WITH WEAK CATION-EXCHANGE PRETREATMENT COLUMN Masatoshi Takahashi *(1), Yosuke Iwata (2), Shuzo Maruyama (2), Yoshiaki Sato (3), Naoki Asakawa
(4) (1) Shimadzu Marketing Center, Shimadzu Corporation,7102 Riverwood Drive, Columnbia, Maryland 21046 USA; (2) LC Business Unit, Analytical Division, Shimadzu Corporation, Nishinokyo Kuwabara-cho, Nakagyo-ku, Kyoto 604-8511, Japan; (3) Analytical Research Laboratories, Eisai Co. Ltd., Tokodai, Tsukuba, Ibaraki 300-2635, Japan; (4) Drug Development Technology, Eisai Co. Ltd., Tokodai, Tsukuba, Ibaraki 300-2635, Japan
PT229 IDENTIFICATION OF IMINIUM ION REACTIVE INTERMEDIATES USING HIGH MASS
ACCURACY MSn ANALYSIS Masatoshi Takahashi *(1), Ichiro Hirano (2), Shin-ichi Yamaguchi (2), Satoshi Yamaki (2), John
Warrander (3), Neil Loftus (3) (1) Shimadzu Marketing Center, Shimadzu Corporation,7102 Riverwood Drive, Columbia, Maryland 21046 USA; (2) Analytical Division, Shimadzu Corporation, Nishinokyo Kuwabara-cho, Nakagyo-ku, Kyoto 604-8511, Japan; (3) Shimadzu Manchester, Mill Court, Featherstone Road, Wolverton Mill South, Milton Keynes MK12 5RD UK
PT230 ULTRA FAST LIQUID CHROMATOGRAPHY FOR THE DETERMINATION OF
GINSENOSIDES IN PANAX GINSENG WITH ETHER-LINKED PHENYL COLUMN Ken-ichiro Tanaka *(1), Tadayuki Yamaguchi (1), Takeshi Goto (1), Hirohisa Mikami (1), Stanley M.
Lok (2) (1) Analytical Applications Department, Shimadzu Corporation, Horiyamashita, Hadano, Kanagawa 259-1304, Japan; (2) Phenomenex Inc, 411 Madrid Avenue, Torrance, CA 90501-1430, USA
PT231 GINKGO ANALYSIS BY USING HPLC-DAD-ELSD Zhixiu Xu (1), Herbert Godel (2)
(1) Agilent Technologies, Shanghai, China; (2) Agilent Technologies, Deutschland GmbH, Waldbronn, Germany
PT232 PURIFICATION OF GINKGO ACID BY USING AGILENT PREPARATIVE HPLC SYSTEM Zhixiu Xu (1), Herbert Godel (2)
(1) Agilent Technologies, Shanghai, China; (2) Agilent Technologies, Deutschland GmbH, Waldbronn, Germany
PT233 STUDY OF ULTRA FAST ANALYSIS OF PHARMACEUTICAL PRODUCTS USING LC-MS Takahiro Goda *, Taku Tsukamoto, Satoshi Yamaki, Naoki Hamada
Analytical Applications Department, Shimadzu Corporation, Horiyamashita, Hadano, Kanagawa 259-1304, Japan
PT234 DETERMINATION OF THE METABOLITES OF ARECOLINE BY MALDI-TOF Chia-Hsien Feng (1), Chi-Yu Lu *(2)
(1) Department of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan; (2) Department of Biochemistry, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan
PT235 RAPID, SENSITIVE AND SIMULTANEOUS DETERMINATION OF
FLUORESCENCE-LABELED DESIGNATED DRUGS BY ULTRA-PERFORMANCE LIQUID CHROMATOGRAPHY COUPLED WITH ELECTROSPRAY-IONIZATION TIME-OF-FLIGHT MASS SPECTROMETRY
Jun Zhe Min *(1), Suguru Hatanaka (1), Toshimasa Toyo'oka (1), Shinsuke Inagaki (1), Ruri Kikura-Hanajiri (2), Yukihiro Goda (2) (1) Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, and Global COE Program, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan; (2) Division of Pharmacognosy, Phytochemistry and Narcotics, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan
Sample Preparation PT236 A POTENTIAL PRE-SEPARATION METHOD FOR PROTEOMICS BY AQUEOUS
TWO-PHASE EXTRACTION COMBINED HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Hao Qin (1), Min Dong (1), Feng Qu *(1), Xinying Zhao (2), Jinghua Zhang (2), Dongxu Zhao (1), Yulin Deng (1), Yukui Zhang (3) (1) School of Life Science & Technology, Beijing Institute of Technology, Beijing 100081, China; (2) Beijing Center for Physical and Chemical Analysis, Beijing 100089, China; (3) Dalian Institute of Chemical Physics, the Chinese Academy of Sciences, Dalian 116023, China
PT237 A NOVEL SOLID-PHASE MICROEXTRACTION FIBER BASED ON THE HYDROGEN
BONDING EXTRACTION FUNCTION Jingbin Zeng *, Xi Chen
Department of Chemistry and the Key Laboratory of Analytical Sciences of the Ministry of Education, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
PT238 APPLICATION OF A NEW SOLID PHASE EXTRACTION DISK BASED ON
SINGLE-WALLED CARBON NANOTUBES SHEET FOR TRACE ANALYSIS OF SOME PERSISTENT TOXIC SUBSTANCES BY HPLC-UV
Hong Yun Niu (1), Ya Qi Cai * (1), Ya Li Shi (1), Fu Sheng Wei (2), Gui Bin Jiang (1) (1) State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Science, Chinese Academy of Sciences, Beijing 100085, China; (2) China National Environmental Monitoring Center, Beijing 100029, China
PT239 CLASS-SPECIFIC IMMUNOAFFINITY MONOLITH FOR ONE-STEP ONLINE
CLEAN-UP OF PYRETHROIDS IN COMPLEX MATRICES FOLLOWED BY HPLC ANALYSIS
Shuang Zhou, Meiping Zhao * College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China
PT240 HIGH THROUGHPUT ON-LINE CONCENTRATION BY NOVEL SEPARATION MEDIA,
NAMED “SPONGY-MONOLITH” Takuya Kubo *, Fuminori Watanabe, Ken Hosoya
Graduate School of Environmental Studies, Tohoku University, Aoba 6-6-20, Aramaki, Aobaku, Sendai 980-8579, Japan
PT241 APPLICATION OF C18 MODIFIED SBA-15 IN ONLINE SOLID-PHASE EXTRACTION
COUPLED WITH HIGH PERFORMANCE LIQUID CHROMATOGRAPHY Tehlong Lai (1), Mengyao Hsieh (1), Chenbin Wang (2), Younyuen Shu *(1)
(1) Department of Chemistry, National Kaohsiung Normal University, Kaohsiung 824, Taiwan; (2) Department of Applied Chemistry and Materials Science, National Defense University, Taoyuan 335, Taiwan
PT242 ON-LINE SPE-HPLC USING RESTRICTED ACCESS PRE-COLUMNS FOR THE
ANALYSIS OF PHENOLS IN HUMIC-CONTAINING WATERS Jiping Ma *, Mo Li, Ronghui Xiao, Wenhui Lu
Key Lab of Environmental Engineering in Shandong Province, Institute of Environment & Municipal
Engineering, Qingdao Technological University, Qingdao 266033, China PT243 HOMOGENEOUS LIQUID-LIQUID EXTRACTION ACTIVE COMPONENTS FROM
METHANOL EXTRACT OF SOME SALVIA MILTIORRHIZA COMPOUND PREPARATIONS PRIOR TO ANALYSIS BY HPLC
Hongyi Zhang *, Cong Jing, Zhihong Shi College of Chemistry and Environmental Science, Hebei University, Baoding, 071002, China
PT244 DEVELOPMENT OF VIAL WALL SORPTIVE EXTRACTION AND ITS APPLICATION TO
DETERMINATION OF PROGESTERONE IN HUMAN SERUM SAMPLE Migaku Kawaguchi *(1), Shin-ichiro Fujii (1), Nobuyasu Itoh (1), Rie Ito (2), Hiroyuki Nakazawa (2),
Akiko Takatsu (1) (1) National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Umezono, Tsukuba, Ibaraki 305-8563, Japan; (2) Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan
PT245 THE DETERMINATION OF AMMONIA BY HEADSPACE SINGLE-DROP
MICROEXTRACTION (SDME) AND ION CHROMATOGRAPHY (IC) Yi-Ping Hang *
School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, 510640, China
PT246 DIRECTLY SUSPENDED DROPLET THREE LIQUID PHASE MICROEXTRACTION OF
3-NITROANILINE IN ENVIRONMENTAL WATER SAMPLES USING 18-CROWN-6 ETHER PRIOR TO HPLC
Ali Sarafraz Yazdi*, Farideh Mofazzeli, Zarrin Es'haghi Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Iran
PT247 A NOVEL SAMPLING APPROACH FOR IDENTIFICATION AND QUANTIFICATION OF
METHYL JASMONATE IN LEAF TISSSUE OF OILSEED RAPE FOR ANALYSIS OF EARLY SIGNALING IN SCLEROTINIA SCLEROTIORUM RESISTANCE
Fang Wei *(1, 2), Ji-Hua Cheng (1), Jun-Yan Huang (1), Sheng-Yi Liu (1), Xu-Yan Dong (1), Ping-Ping Li (1), Yan-Hua Li (1), Hong Chen (1, 2) (1) Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, Hubei 430062, PR China; (2) Key Laboratory for Genetic Improvement of Oil Crops, Ministry of Agriculture, PR China
PT248 ELECTRO MEMBRANE ISOLATION OF NERVE AGENT DEGRADATION PRODUCTS
ACROSS A SUPPORTED LIQUID MEMBRANE FOLLOWED BY CAPILLARY ELECTROPHORESIS-CAPACITIVELY COUPLED CONTACTLESS CONDUCTIVITY DETECTION AND DETERMINATION
Li Xu *(1), Peter C. Hauser (2), Hian Kee Lee (1) (1) Department of Chemistry, University of Singapore, 3 Science Drive 3, Singapore 117543; (2) Department of Chemistry, University of Basel, Spitalstrasse 51, 4056 Basel, Switzerland
PT249 COMBINATION OF SOLID PHASE NANOEXTRACTION AND CAPILLARY
ELECTROPHORESIS FOR THE ANALYSIS OF 1,2- AND 1,3- DIOL COMPOUNDS Peng Dou *, Liang Liang, Zhen Liu
Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China
PT250 REACTIVE MATRIX CLEAN UP OF POLYNUCLEAR AROMATIC COMPOUNDS IN THE
ENVIRONMENT Torsten Spitzer *
Environmental, Industrial and Food Analysis, Postfach 1428, D-38334 Helmstedt PT251 A DYNAMIC, REACTIVE AND PASSIVE MICRO-MEMBRANE SAMPLING FOR
DETERMINATION OF TRACE FORMALDEHYDE IN AIR Xiyun Luo (1, 2), Yuan Mei *(1), Yiping Du (1), Weibing Zhang (1)
(1) School of Chemistry and Molecular Engineering, East China University of Science and Technology, Mei Long Rd130, Shanghai, China; (2) Laboratory of Sciences of Conservation and Archaeology, Shanghai Museum, West Yan An RD1357, Shanghai, China
PT252 IMPROVED TECHNIQUE OF PRETREATMENT METHOD FOR ENVIRONMENTAL
WATER ANALYSIS USING COLUMN SWITCHING LC-MS Tairo Ogura *(1), Yoshiyuki Watabe (1), Tomio Fujita (1), Takuya Kubo (2), Ken Hosoya (2),
Kunimitsu Kaya (2) (1) Analytical Applications Department, Shimadzu corporation, 1, Nishinokyo- kuwabaracho, Nakagyo-ku, Kyoto 604-8511, Japan; (2) Graduate School of Environmental Studies, Tohoku University, Aoba 6-6-20, Aramaki, Aoba-ku, Sendai 980-8579, Japan
PT253 PROFILING OF TRYPTOPHAN METABOLITES BY HIGH SPEED AND HIGH
RESOLUTION HPLC WITH AN AUTOMATED COLUMN SWITCHING PRETREATMENT SYSTEM
Junichi Masuda *(1), Yoshihiro Hayakawa (1), Shuzo Maruyama (1), Kuniaki Saito (2) (1) Shimadzu Corporation, Nakagyo-ku, Kyoyo 604-8511, Japan; (2) Graduate School of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8507
PT254 DETERMINATION OF IMPURITIES IN PHRAMACEUTICAL PRODUCTS BY ONLINE
PRE-TREATMENT SYSTEM COMBINED WITH LCMS-IT-TOF MASS SPECTROMTER Okiyuki Kunihiro *, Yoshihiro Hayakawa, Shuzo Maruyama
Shimadzu Corporation, Nakagyo-ku, Kyoto 604-8511, Japan PT255 DETERMINATION OF POLYBROMOBIPHENYLS AND POLYBROMODIPHENYL
ETHERS IN ELECTRICAL AND ELECTRONIC EQUIPMENTS BY GAS CHROMATOGRAPHY MASS SPECTROMETRY BASED ON MICROWAVE-ASSISTED EXTRACTION METHOD
Ying Li *(1), Yuki Hashi (1), Tian-Ran Wang (1), Jin-Ming Lin (1, 2) (1) Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China; (2) Department of Chemistry, Tsinghua University, Beijing 100084, China
PT256 GENERIC METHODS FOR DIURETIC DETERMINATION IN URINE USING LogD AND
pH PREDICTION—LIQUID/LIQUID OR SOLID PHASE EXTRACTION WITH SPE CARTRIDGES AND HPLC ANALYSIS
Charles Lévesque, François Béland * SiliCycle Inc., 114-1200, St-Jean-Baptiste Avenue, Quebec City, (Quebec), Canada G2E 5E8