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Test Directory Effective 1/1/2020 Phoenix Lab | 4338 Harbour Point Blvd. SW | Mukilteo, WA 98275 main: 425.355.5252 | toll free: 800.347.0043 | fax: 425.355.3676 www.phoenixlab.com

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Page 1: Test Directory - Phoenix Lab · 2020-01-08 · Test Directory Effective 1/1/2020 Phoenix Lab | 4338 Harbour Point Blvd. SW | Mukilteo, WA 98275 main: 425.355.5252 | toll free: 800.347.0043

Test Directory Effective 1/1/2020

Phoenix Lab | 4338 Harbour Point Blvd. SW | Mukilteo, WA 98275

main: 425.355.5252 | toll free: 800.347.0043 | fax: 425.355.3676

www.phoenixlab.com

Page 2: Test Directory - Phoenix Lab · 2020-01-08 · Test Directory Effective 1/1/2020 Phoenix Lab | 4338 Harbour Point Blvd. SW | Mukilteo, WA 98275 main: 425.355.5252 | toll free: 800.347.0043

Index

Submission Guidelines............................................................ 1

Canine PanelsACTH Response....................................................................... 3Adult Routine Screen............................................................... 4Antinuclear Antibody.............................................................. 4Autoimmune Panel.................................................................. 5Basic Canine Chemistry.......................................................... 5Basic Health Profile.................................................................. 6Bile Acids Panel........................................................................ 6Bile Acids, Single Sample........................................................ 7Blood Typing, Canine............................................................ 7Brucella Screen, Canine........................................................... 7Canine Breeding Panel............................................................ 8Canine GI Panel (cPL/TLI/Cobalamin/Folate).................... 8Canine Heartworm Panel........................................................ 9Canine Immunization Screen................................................. 9Canine PLI and TLI................................................................ 10Canine PLI/Cobalamin/Folate............................................. 10CBC w/ Differential............................................................... 10CBC, Follow-up...................................................................... 11CBC, Platelet if Indicated...................................................... 11Chemistry Screen................................................................... 11Chemistry Screen, Follow-up............................................... 12Coagulation Panel I................................................................ 12Coagulation Panel II.............................................................. 13Coagulation Panel III............................................................. 13Cobalamin (Vitamin B12).................................................... 14Cobalamin and Folate........................................................... 14Coombs................................................................................... 14Cortisol, Resting..................................................................... 15Canine PLI.............................................................................. 15Crossmatch Complete........................................................... 16Dexamethasone Suppression................................................ 17Distemper, FA........................................................................ 18Electrolytes Screen................................................................. 18Endocrine Panel..................................................................... 19Ethylene Glycol....................................................................... 19Fecal Parasite Combo w/ Giardia/Crypto IFA .................. 20Fecal Parasite Screen.............................................................. 20Folate........................................................................................ 21Free T4..................................................................................... 21Free T4, Add-on..................................................................... 22

General Diagnostic w/ Thyroid II........................................ 23

General Diagnostic................................................................. 22

General Diagnostic w/ Triglycerides................................... 24

General Diagnostic w/ T4..................................................... 23

General Diagnostic w/ T4 and Cobalamin/Folate............. 24

General Diagnostic w/ T4, Follow-up................................. 25

General Diagnostic w/ Coagulation I.................................. 25General Diagnostic, Follow-up............................................. 26General Endocrine, Canine................................................... 27Giardia ELISA......................................................................... 28Giardia/Cryptosporidium IFA............................................ 28Heartworm Antigen............................................................... 28Heartworm Antigen, Add-on............................................... 29IgA Quantitative, Canine...................................................... 29Immune Mediated Hemolytic Anemia (IMHA) Panel..... 30Insulin...................................................................................... 30Juvenile Routine Screen......................................................... 31Leptospirosis Rapid Screen................................................... 31Liver Chemistry Panel........................................................... 32Liver Profile............................................................................. 33Luteinizing Hormone............................................................ 34Microfilaria.............................................................................. 34Oncology Profile..................................................................... 35Ova & Parasite......................................................................... 35Ova & Parasite Combo w/ IFA............................................. 36Parvovirus Antibody, Serum................................................. 36Parvovirus Antigen, Fecal..................................................... 36Phenobarbital.......................................................................... 37Phenobarbital, Peak & Trough............................................. 38Pre-Op Panel........................................................................... 39Pre-Op Panel, Follow-up....................................................... 39Progesterone, Canine............................................................. 40Progesterone, Follow-up....................................................... 40Protein Electrophoresis......................................................... 41Relaxin, Canine or Feline...................................................... 41Relocated Canine Routine Screen........................................ 42Renal Panel, Chronic............................................................. 42Renal Profile............................................................................ 43Renal Profile w/ Urinalysis.................................................... 43Resting Cortisol...................................................................... 44Senior Routine Screen............................................................ 44Senior Screen........................................................................... 45Senior Screen w/ CBC............................................................ 45Senior Screen w/ cTSH.......................................................... 46Senior Screen w/ Cholesterol................................................ 46T3 (Triiodothyronine)........................................................... 47Thyroid I.................................................................................. 47Thyroid II................................................................................ 47Thyroid III............................................................................... 48Thyroid IV............................................................................... 48Thyroid Screen........................................................................ 49TLI, Canine.............................................................................. 49TLI/Cobalamin/Folate (Canine Only)................................. 50Total Body Diagnostic........................................................... 50

Page 3: Test Directory - Phoenix Lab · 2020-01-08 · Test Directory Effective 1/1/2020 Phoenix Lab | 4338 Harbour Point Blvd. SW | Mukilteo, WA 98275 main: 425.355.5252 | toll free: 800.347.0043

Total Body Diagnostic w/o Urinalysis................................. 51

Total Body Diagnostic, Follow-up....................................... 52TSH (Thyroid Stimulation Hormone)................................ 52Urinalysis................................................................................. 53Urinalysis w/ Culture and Sensitivity.................................. 53

Urine Cortisol/Creatinine Ratio.......................................... 54

Urine Protien Electrophoresis.............................................. 55Vector/Tick Borne Disease Screen....................................... 56Wellness Screen II.................................................................. 56Wellness Screen II w/ SDMA........................................ 57Wellness Screen III........................................................ 57Wellness Screen IV........................................................ 58Wellness/Geriatric Panel............................................... 58

Wellness/Geriatric Panel w/ Triglycerides................... 59Wellness Geriatric Panel, Follow-up............................ 60

Wellness/Geriatric Panel w/ SDMA............................. 59

Feline Panels

Total Body Diagnostic w/o Urinalysis, Follow-up............. 51

ACTH Response, Feline................................................. 61Adult Routine Screen..................................................... 62Antinuclear Antibody............................................................ 62Autoimmune Panel................................................................ 63Basic Feline.............................................................................. 63Basic Health Profile......................................................... 64Bile Acids, Single Sample...................................................... 64Blood Typing, Feline.............................................................. 65CBC w/ Differential............................................................... 65CBC, Follow-up...................................................................... 65CBC, Platelet if Indicated...................................................... 65Chemistry Screen................................................................... 66Chemistry Screen w/ T4 and Feline Viral II....................... 66Chemistry Screen, Follow-up............................................... 67Coagulation Panel I................................................................ 67Coagulation Panel II.............................................................. 68Coagulation Panel III............................................................. 68Cobalamin (Vitamin B12)..................................................... 69Cobalamin and Folate............................................................ 69Coombs.................................................................................... 69Cortisol, Resting..................................................................... 70Crossmatch Complete........................................................... 70Electrolytes Screen................................................................. 71Ethylene Glycol....................................................................... 71Fecal Parasite Combo w/ Giardia Crypto IFA................... 71Fecal Parasite Screen.............................................................. 72Feline ADR Panel................................................................... 72Feline Calicivirus Titer.......................................................... 73Feline Coronavirus (FIP) Screen.......................................... 73Feline Coronavirus (FIP) Screen, Add-on.......................... 74Feline Coronavirus (FIP), Titered........................................ 74Feline Heartworm Panel........................................................ 75

Feline Heartworm Screen...................................................... 75Feline Herpesvirus Titer.................................................... 75Feline Immunization Screen I.......................................... 76Feline Immunization Screen II......................................... 76Feline Immunization Screen III........................................... 77Feline Panel............................................................................. 77Feline Panleukopenia Virus Titer........................................ 78Feline Screen........................................................................... 78Feline TLI/PLI/Cobalamin/Folate....................................... 79Feline Toxoplasmosis IgG/IgM............................................ 79Feline Viral I........................................................................... 80Feline Viral II.......................................................................... 80Feline Viral III........................................................................ 81FeLV (ELISA)......................................................................... 81FeLV, Add-on......................................................................... 82FIV........................................................................................... 82FIV, Add-on............................................................................ 82Folate....................................................................................... 83Feline PLI/Cobalamin/Folate............................................... 83Free T4..................................................................................... 84General Diagnostic................................................................ 84General Diagnostic w/ Feline Viral I................................... 85General Diagnostic w/ Feline Viral II................................. 85

General Diagnostic w/ T4 and Feline Viral II.................... 87General Diagnostic w/ T4 and FeLV................................... 87General Diagnostic w/ Triglycerides................................... 88

General Diagnostic w/ T4..................................................... 86General Diagnostic w/ T4 and Feline Viral I..................... 86

General Diagnostic W/ T4 and Cobalamin/Folate........... 88General Diagnostic w/ T4, Follow-up................................. 89General Diagnostic w/ Coagulation I.................................. 89General Diagnostic, Follow-up............................................ 90Giardia, ELISA........................................................................ 90Giardia Cryptosporidium IFA............................................. 90Heartworm Antibody, Feline............................................... 91Immune Mediated Hemolytic Anemia (IMHA) Panel.... 91Juvenile Routine Screen........................................................ 92Liver Chemistry Panel........................................................... 92Liver Profile............................................................................ 93Luteinizing Hormone............................................................ 94Microfilaria............................................................................ 94Mycoplasma haemofelis (Hemobartonella)....................... 95Oncology Profile.................................................................... 95Ova & Parasite........................................................................ 95Ova & Parasite Combo w/ IFA............................................ 96Panleukopenia, Fecal............................................................. 96Pre-Op Panel.......................................................................... 96Pre-Op Panel, Follow-up...................................................... 97Protein Electrophoresis......................................................... 97Relaxin, Canine or Feline...................................................... 98Relocated Feline Routine Screen......................................... 99Renal Panel, Chronic............................................................. 99Renal Profile......................................................................... 100 Renal Profile w/ Urinalysis................................................. 100Senior Routine Screen......................................................... 101Senior Screen........................................................................ 101

Urinalysis w/ Culture, Follow-Up........................................ 54

Urine Culture, Follow-Up .................................................... 55

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Senior Scree w/ CBC............................................................ 102Senior Screen w/ Cholesterol.............................................. 102T3 (Triiodothyronine)......................................................... 103T3 Suppression I................................................................... 103T3 Suppression II................................................................. 104Thyroid II.............................................................................. 104Thyroid Screen..................................................................... 105TLI/Cobalamin/Folate (Feline Only)................................ 105Total Body Diagnostic......................................................... 106Total Body Diagnostic w/o Urinalysis.............................. 106Total Body Diagnostic w/o Urinalysis, Follow-up.......... 107Total Body Diagnostic, Follow-up.................................... 107Urinalysis.............................................................................. 108Urinalysis w/ Culture and Sensitivity............................... 108

Urine Cortisol/Creatinine Ratio........................................ 109

Urine Protein Electrophoresis........................................... 110Vector/Tick Borne Disease Screen.................................... 111Wellness Screen II................................................................ 111Wellness Screen II w/ SDMA............................................. 112Wellness Screen III.............................................................. 112Wellness Screen IV.............................................................. 113Wellness/Geriatric Panel.................................................... 113Wellness/Geriatric Panel w/ SDMA.................................. 114Wellness/Geriatric Panel w/ Triglycerides....................... 114Wellness/Geriatric Panel, Follow-up................................ 115Wellness/Geriatric Panel w/ Feline Viral I....................... 115Wellness/Geriatric Panel w/ Feline Viral II..................... 116

Equine/Large Animal PanelsACTH Plasma, Endogenous............................................... 117Bovine Chemistry Panel...................................................... 117Bovine Panel for Calcium Supplement............................. 118CBC w/ Fibrinogen and Plasma Protein........................... 118CBC w/ Plasma Protein....................................................... 118Chemistry Screen, Large Animal....................................... 119Coggins EIA, AGID............................................................. 119Coggins EIA, ELISA............................................................ 120Culture, Equine Uterine...................................................... 120Dexamethasone Suppression, Equine............................... 121Equine Chemistry Screen.................................................... 121Equine General Endocrine................................................. 122Equine Immunodeficiency................................................. 122Equine Insulin/ACTH, Endogenous................................. 123Equine Pre-Op Panel........................................................... 123Equine Screen....................................................................... 124Equine Screen, Follow-up................................................... 124Equine Senior Wellness I.................................................... 125Equine Senior Wellness II................................................... 126Equine Special w/ Insulin................................................... 127Equine Total Body............................................................... 128Fecal Parasite, Quantitative................................................ 128Fibrinogen, Semi-quantitative........................................... 128IgG, Camelid......................................................................... 129IgG, Equine........................................................................... 129

IgM, Equine.......................................................................... 129Insulin................................................................................ 130Large Animal Liver Chemistry.......................................... 130Occult Blood........................................................................ 131Pre-Op Panel, Large Animal.............................................. 131Progesterone, Non-Canine................................................. 132Ruminant Screen................................................................. 132Ruminant Screen, Follow-up............................................. 133Sorbitol Dehydrogenase (SDH)......................................... 133Special Equine Profile......................................................... 134T3 (Triiodothyronine)........................................................ 134TRH Stimulation Test........................................................ 135Urinalysis............................................................................. 135Uterine Screen, Equine....................................................... 136

Avian and Exotic PanelsAvian and Exotic Sample Guidelines................................ 137Avian Liver Chemistry........................................................ 138Avian Mini Liver Panel....................................................... 138Avian Mini Renal Panel...................................................... 138Avian Panel........................................................................... 139Avian Panel, Follow-up....................................................... 139Avian Renal Chemistry....................................................... 140Avian/Nonmammalian 13-Chemistry Screen................. 140Avian/Nonmammalian 10-Chemistry Screen................. 140Avian/Nonmammalian 13-Chemistry w/ CBC............... 141Avian/Nonmammalian 13-Chemistry

w/ CBC and Protein Electrophoresis............................. 141Avian/Nonmammalian 13-Chemistry

w/ CBC, Follow-up........................................................... 142Avian/Nonmammalian 18-Chemistry Screen................. 142Avian/Nonmammalian 18-Chemistry w/ CBC............... 143Avian/Nonmammalian 18-Chemistryw/ CBC and Protein Electrophoresis................................ 144Avian/Nonmammalian 21-Chemistry.............................. 144Avian/Nonmammalian 21-Chemistry w/ CBC............... 145Avian/Nonmammalian 21-Chemistry

w/ CBC and Protein Electrophoresis............................. 146Avian/Reptile CBC............................................................... 146Avian/Reptile CBC, Follow-up........................................... 147Avian/Reptile Giardia/Crypto w/ Modified Acid Fast.... 147Reptile Mini Liver Panel...................................................... 147Reptile Mini Renal Panel..................................................... 148Reptile Profile....................................................................... 148Reptile Profile, Follow-up................................................... 149

Small Mammal PanelsCBC, Exotic/Small Mammal............................................... 151CBC, Platelet if Indicated.................................................... 151Small Mammal Chemistry Screen I................................... 151Small Mammal Chemistry Screen II.................................. 152Small Mammal Comprehensive Chemistry Screen......... 152Small Mammal Comprehensive Panel.............................. 153Small Mammal Comprehensive Panel

w/ Protein Electrophoresis.............................................. 154

Urinalysis w/ Culture, Follow-Up..................................... 109

Urine Culture, Follow-Up.................................................. 110

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Small Mammal Comprehensive Panel, Follow-up.......... 155Small Mammal Mini Liver Panel....................................... 155Small Mammal Mini Renal Panel...................................... 156Small Mammal Panel I........................................................ 156Small Mammal Panel I, Follow-up.................................... 157Small Mammal Panel II....................................................... 157Small Mammal Panel II w/ Protein Electrophoresis....... 158Small Mammal Panel II, Follow-up................................... 158

Cytology PanelsCytology Submission Guidelines....................................... 159Body Cavity/Joint Fluid Cytology...................................... 161Body Cavity/Joint Fluid Cytology, Additional Source.... 161Bone Marrow Cytology....................................................... 162Bone Marrow Cytology w/ CBC........................................ 163Buffy Coat Cytology............................................................. 164CSF Analysis......................................................................... 164CSF Analysis, Multiple Sites............................................... 164CSF Panel, Special................................................................ 165Cytology................................................................................ 166Cytology Slide Prep Only.................................................... 167Mini-Cytology...................................................................... 168Semen Evaluation................................................................. 169

EndocrinologyACTH Plasma, Endogenous............................................... 171ACTH Response................................................................... 172ACTH Response, Feline...................................................... 173Basic Feline........................................................................... 173Basic Health Profile............................................................. 174Canine Breeding Panel........................................................ 174Chemistry Screen w/ T4 and Feline Viral II..................... 175Cortisol, Resting................................................................... 175Cortisol, Urine...................................................................... 176Dexamethasone Suppression............................................. 176Dexamethason Suppression, Equine................................. 177Endocrine Panel................................................................... 178Equine General Endocrine................................................. 179Equine Insulin/ACTH, Endogenous................................. 179Equine Senior Wellness I.................................................... 180Equine Senior Wellness II................................................... 181Equine Special w/ Insulin.................................................... 182Feline Panel........................................................................... 183Free T4................................................................................... 183Free T4, Add-on................................................................... 184General Diagnostic w/ Thyroid II...................................... 184General Diagnostic w/ T4 and Feline Viral II.................. 185General Diagnostic w/ T4 and FeLV................................. 185General Diagnostic w/ T4................................................... 186General Diagnostic w/ T4 and Feline Viral I................... 186General Diagnostic w/ T4 and Cobalamin/Folate........... 187General Diagnostic w/ T4, Follow-up............................... 187General Endocrine, Canine................................................ 188Insulin.................................................................................... 189Lipid Panel w/ T4................................................................. 189Luteinizing Hormone.......................................................... 190

Progesterone, Canine........................................................ 191Progesterone, Follow-up..................................................... 191Progesterone, Non-Canine................................................. 192Relaxin, Canine or Feline.................................................... 192Resting Cortisol.................................................................... 193RX - Methimazole I.............................................................. 193RX - Methimazole II............................................................. 194RX - Thyroxine/Soloxine..................................................... 194RX - Cushing's (Trilostane, Mitotane).............................. 195Senior Routine Screen.......................................................... 196Senior Screen......................................................................... 196Senior Screen w/ Calcium and HCT.................................. 197Senior Screen w/ CBC and Free T4.................................... 197Senior Screen w/ TSH.......................................................... 198Senior Screen w/ Free T4..................................................... 198Senior Screen w/ Calcium................................................... 199Senior Screen w/ Cholesterol.............................................. 199Special Equine Profile.......................................................... 200T3 (Triiodothyronine)......................................................... 200T3 Suppression I................................................................... 201T3 Suppression II.................................................................. 202Thyroid I................................................................................ 202Thyroid II.............................................................................. 203Thyroid III............................................................................. 203Thyroid IV............................................................................. 204Thyroid Panel V.................................................................... 204Thyroid Screen...................................................................... 205Total T4.................................................................................. 205Total T4, Add-on.................................................................. 205TRH Stimulation Test.......................................................... 206TSH (Thyroid Stimulation Hormone).............................. 206TSH, Add-on......................................................................... 207Urine Cortisol/Creatinine Ratio......................................... 207Urine, Cortisol...................................................................... 207Wellness Screen II................................................................ 208Wellness Screen II w/ SDMA.............................................. 208Wellness Screen II w/ Urine to Follow.............................. 209Wellness Screen III............................................................... 209Wellness Screen IV............................................................... 210Wellness/Geriatric Panel..................................................... 210Wellness/Geriatric Panel w/ SDMA.................................. 211Wellness/Geriatric Panel w/ Triglycerides........................ 211Wellness/Geriatric Panel, Follow-up................................. 212Wellness/Geriatric Panel w/ Feline Viral I........................ 212Wellness/Geriatric Panel w/ Feline Viral II...................... 213

HistopathologyHistopathology Submission Guidelines............................ 215Histopathology Single Tissue -

Mini Path Review, Slides Only........................................ 217Histopathology Single Tissue -

Full Path Review, Slides Only.......................................... 217Histopathology Complex Tissue -

Full Path Review, Slides Only......................................... 218

Panels

Page 6: Test Directory - Phoenix Lab · 2020-01-08 · Test Directory Effective 1/1/2020 Phoenix Lab | 4338 Harbour Point Blvd. SW | Mukilteo, WA 98275 main: 425.355.5252 | toll free: 800.347.0043

Histopathology Additional Skin Punches - Full, Slides Only............................................................ 218

Histopathology Add'l SingleTissue - Full, Slides Only............................................................ 219

Histopathology Add'l Single Tissue x2 - Full, Slides Only............................................................ 219

Histopathology Add'l Single Tissue x3 - Full, Slides Only............................................................... 220

Histopathology Add'l Single Tissue x4 - Full, Slides Only.............................................................. 220

Histopathology Skin Punch Biopsies - Mini, Slides Only............................................................ 221

Histopathology Skin Punch Biopsies - Full, Slides Only.............................................................. 221

Histopathology Additional Skin Punches - Mini, Slides Only............................................................ 221

Histopathology Add'l Single Tissue - Mini, Slides Only............................................................ 222

Histopathology Add'l Single Tissue x2 - Mini, Slides Only............................................................ 222

Histopathology Add'l Single Tissue x3 - Mini, Slides Only............................................................ 223

Histopathology Add'l Single Tissue x4 - Mini, Slides Only............................................................ 223

Histopathology Complex Tissue - Mini Path Review, Slides Only..................................... 224

Histopathology Single Tissue - Mini Report................... 224Histopathology Single Tissue - Full Report..................... 225Histopathology Complex Tissue - Full Report................ 225Histopathology Complex Tissue x2 - Full Report........... 226Histopathology Complex Tissue x3 - Full Report........... 226Histopathology Complex Tissue x4 - Full Report........... 227Histopathology Necropsy................................................... 227Histopathology Limb Dissection....................................... 228Histopathology Additional Skin Punch Biopsies -

Full Report...................................................................... 228Histopathology Additional Single Tissue -

Full Report...................................................................... 229Histopathology Additional Single Tissue x2 -

Full Report...................................................................... 229Histopathology Additional Single Tissue x3 -

Full Report...................................................................... 230Histopathology Additional Single Tissue x4 -

Full Report...................................................................... 230Histopathology Skin Punch Biopsies - Mini Report....... 231Histopathology Skin Punch Biopsies - Full Report........ 231Histopathology Additional Skin Punch Biopsies -

Mini Report.................................................................... 232Histopathology Additional Single Tissue -

Mini Report.................................................................... 232Histopathology Additional Single Tissue x2 -

Mini Report.................................................................... 233Histopathology Additional Single Tissue x3 -

Mini Report.................................................................... 233Histopathology Additional Single Tissue x4 -

Mini Report.................................................................... 234

Histopathology Complex Tissue - Mini Report............. 234Histopathology Complex Tissue x2 - Mini Report......... 235Histopathology Complex Tissue x3 - Mini Report.......... 235Histopathology Special Stain.............................................. 236Histopathology Special Stain x2......................................... 236Histopathology Special Stain x3......................................... 236Histopathology Special Stain x4......................................... 237

Microbiology PanelsMicrobiology Submission Guidelines.............................. 239Acid Fast Stain...................................................................... 241Add-on Sensi/Yeast/Topicals............................................. 241Culture & Sensitivity, Aerobic........................................... 242Culture & Sensitivity, Milk................................................. 242Culture ID/Sensitivity if Indicated, Avian........................ 242Culture, Aerobic ID Only................................................... 243Culture, Aerobic ID only, Anaerobic ID only.................. 243Culture, Aerobic w/ Sensitivity-

Anaerobic Culture, ID only....................................... 243Culture, Anaerobic (ID Only)............................................ 244Culture, Blood...................................................................... 244Culture, Dermatology.......................................................... 244Culture, Equine Uterine...................................................... 245Culture, Feces....................................................................... 245Culture, Fungal..................................................................... 245Culture, Mycoplasma.......................................................... 246Culture, Urine....................................................................... 246Dermatophilus Prep............................................................ 246Fecal Baermann.................................................................... 247Fecal Combo Screen w/ Giardia ELISA............................ 247Fecal Parasite Combo w/ Giardia/Crypto IFA................. 247Fecal Parasite Screen............................................................ 248Fecal Parasite, Quantitative................................................ 248Gram Stain............................................................................ 248Modified Acid Fast Stain..................................................... 248Ova & Parasite...................................................................... 249Ova and Parasite Combo w/ IFA....................................... 249Tritrichomonas Fecal.......................................................... 250Urinalysis w/ C&S................................................................ 250Urinalysis w/ Culture, Follow-up...................................... 251Urine Culture & Sensitivity, Add-on................................ 251Urine Culture, Follow-up................................................... 252

Test List, Alphabetical................................ 253

Page 7: Test Directory - Phoenix Lab · 2020-01-08 · Test Directory Effective 1/1/2020 Phoenix Lab | 4338 Harbour Point Blvd. SW | Mukilteo, WA 98275 main: 425.355.5252 | toll free: 800.347.0043

Sample Collection and Submission To get the most out of diagnostic testing, it is critical that samples are handled and submitted properly. Phoenix Lab is here to help. Read on for general sample submission guidelines and be sure to review any special submission guidelines for individual tests.

Before Collection • Please examine all specimen collection and transportation supplies to make sure they are

not expired.• When collecting blood samples, select the appropriate size syringe and needle to ensure

enough sample is collected and to help avoid hemolysis. When possible, use a 22g needle orlarger for blood collection; larger gauge needles are recommended for large dogs or largeanimals.

• Make sure you have the proper tubes available. Additives that preserve a sample for onetype of test may actually interfere with a different test (ex: EDTA will cause chemistry resultsto be unreliable).

• Select the proper sized tubes for the anticipated volume – use smaller tubes for smallerpatients. Tubes that are too large can result in inaccurate results due to dilution or “QNS”(quantity not sufficient) errors.

• Ensure your patient is properly prepared for the collection. Ensure patients are fasting whensubmitting fasting specimens or indicate otherwise. Fasting samples are helpful to avoidlipemia which can interfere with analysis. Medications should be administered at the propertime before or during the testing process.

Using the Correct Tube – Common Collection Tube Abbreviations Abbreviation Tube Abbreviation Tube SST Serum separator tube (gel barrier) RBTT Royal blue top tube m-SST Microtainer serum separator tube GRNTT Green top tube (Li Heparin) RTT Red top tube m-GnTT Microtainer green top tube m-RTT Microtainer red top tube LTT Lavender top tube (EDTA) BTT Blue top tube (buffered Na citrate) m-LTT Microtainer lavender top tube GTT Grey top tube (K Oxalate, Na fluoride) Slides Glass slides m-GTT Microtainer Grey top tube Crits Hematocrit tube CTT Clear top tube (no additive, sterile) Urine tube 15 mL conical tube (nonsterile)

Immediately After Collection The easiest and most important way to ensure quality is to properly label the sample. All samples should be labeled with the first and last name of the patient. Labeling date and time of collection is especially important for tests like pre- and post-bile acids, ACTH stimulation tests, or dexamethasone suppression tests. Other post-collection considerations include:

• When transferring the blood from the syringe to the tubes, avoid excessive pressure whichcan cause RBC lysis.Immediately and gently, invert the tube 5-10 times to mix the sample with any additives.

• Allow serum samples to clot for a minimum of 15 minutes before centrifugation. Centrifugeand separate serum or plasma from red cells within 30-60 minutes of collection. Red cells

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Page 8: Test Directory - Phoenix Lab · 2020-01-08 · Test Directory Effective 1/1/2020 Phoenix Lab | 4338 Harbour Point Blvd. SW | Mukilteo, WA 98275 main: 425.355.5252 | toll free: 800.347.0043

can falsely decrease glucose and falsely elevate potassium in some species if not separated quickly. Delayed separation can also lead to hemolysis within the serum, which can interfere with analysis. If the serum/plasma (after centrifugation) is markedly lipemic or markedly hemolyzed, and there is not a known process as the cause (hyperlipidemia, IMHA etc), please collect a fresh sample from the patient.

• Serum or plasma samples with no gel separator should be transferred to a different tubeand labeled “serum” or “plasma.”

• For CBCs, please make 1-2 blood smears as soon as possible after collection and allow to airdry completely before putting into a container for transport to our laboratory and do notrefrigerate. If the CBC sample is clotted (either in the syringe or in the LTT), please collect anew, non-clotted sample from the patient.

Urine Collection The most common urine collection considerations:

• Mark the method of collection – free catch, catheter, or cystocentesis• Store specimens in a urine tube or sterile CTT. Secure lids to avoid leakage.• Store in a cool place, such as a refrigerator.

Outdoor Specimen Lockboxes Samples should stay indoors and stored at the proper temperature until collected by the courier. Evening sample pickups after closing may make this impossible, and samples may be placed outside in a lockbox until the courier arrives. Lockboxes should be insulated with Styrofoam, should close properly, and should not have any rust/damage. If your lockbox needs repair, please notify Phoenix Lab. For clients whose specimen lockboxes must be placed outdoors, adding a frozen gel pack to the lockbox during warmer weather will help provide a moderate temperature inside the lockbox until your Phoenix courier collects the specimens. They should be frozen solid prior to being placed in the lockbox, and they should be frozen solid before each use, even though they may be used with specimens that are not frozen. To avoid freezing the samples, wrap the ice pack in paper towels or other insulating material. Gel packs should be prepared for use by placing them in a freezer for four hours or longer.

Number of Frozen Gel Packs RecommendedApproximate Outdoor Temperature Frozen Gel Packs Recommended

Below 80°F One frozen gel pack 80°F − 100°F Two frozen gel packs 100°F − 120°F Three frozen gel packs

If it is not possible to avoid placing the specimen lockbox in direct sunlight, add 20°F to the temperatures listed above to determine how many frozen gel packs to use.

Cold Weather When temperatures are below freezing, a thawed (room temperature) gel pack in the lock box can help prevent sample freezing.

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Canine Panels

39 ACTH Response

Methodology: Chemiluminescent Immunoassay

Pre Cortisol

Specimen Requirements 0.5 mL Serum each RTTx2; Pre, 1 hour Post. DO NOT USE SST TO COLLECT OR SPIN DOWN SAMPLE

Reported Time Same Day

Post Cortisol

Collection Notes PROTOCOL:1.  Collect a resting sample in a RTT, 0.5 mL of serum is required.  Separate serum if possible and refrigerate. 2.  Inject 0.25 mg (250 µg) of Cortrosyn IM or IV (1 vial). 3.  Collect a second sample EXACTLY 1 HOUR post injection in RTT, label clearly as to the collection status (post), and preserve in the same manner as the first sample.

NOTE: The test has been validated using Cortrosyn. Although compounded gels are available, they vary greatly in peak times and are not recommended.

ALTERNATE CANINE PROTOCOL (Microdose): In order to keep Cortrosyn costs down, use the specific dose of 5 ug/kg IV - up to the maximum dose of 250 ug (one vial) per dog.  Whatever is left over can then be split into aliquots of 25 ug  or 50 ug using small plastic syringes.  Normally 1 mL of sterile physiologic saline is used as diluent.  If so, 25 ug would be 100 uL or 0.1 mL. ***When the patient is receiving Trilostane/Vetoryl, the timing of the test is important.  The pre-cortisol sample should be drawn 3-5 hours after the morning capsule.  The test is a one hour test, so in other words, the ACTH Response test should be completed 4-6 hours after the medication, which is usually given once a day in the morning.

Additional Comments: Pre and Post sample required. In addition to diagnosis of naturally occurring hyperadrenocorticism (Cushing's disease), this is the only diagnostic test for hypoadrenocorticism (Addison's disease), iatrogenic hyperadrenocorticism, and monitoring response to medical therapy of Cushing's disease. Prednisone, prednisolone, and other corticosteroids (except dexamethasone) will cross react. Recommend these drugs be discontinued at least 48 hrs before testing. If dog has been on prolonged steroids, patient must be tapered with the drug stopped for at least 3 weeks to let the adrenal gland recover. INTERPRETATION HYPERADRENOCORTICISM: An ACTH response test is done to CONFIRM clinical suspicion of hyperadrenocorticism. Stressful diseases (ill diabetics, renal failure, etc.) and current drug therapy may cause false positives. If the cortisol results are normal (and an adequate dose of Cortrosyn was administered) and there is high clinical suspicion of hyperadrenocorticism, sex hormone hyperadrenocorticism (atypical Cushing's) should be considered. Additional testing on the serum from this ACTH stimulation test can be performed. Please contact us within 7 days of this cortisol result for further testing options. A low-dose dexamethasone suppression test should also be considered, as some patients with Cushing's will be positive on one test but not on the other.INTERPRETATION HYPOADRENOCORTICISM: Pre and post cortisol <2 ug/dL are supportive of hypoadrenocorticism but need to be interpreted in light of other clinical findings and history. Failure to stimulate can occur with iatrogenic hyperadrenocorticism, dogs being treated for hyperadrenocorticism and Addison's disease.

For Feline Protocol See ACTH Response, Feline 1039

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1719 Adult Routine Screen

CBCAlbuminALP (Alkaline Phosphatase)BUN (Blood Urea Nitrogen)CreatinineUrinalysis

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urine

Reported Time Same Day

BUN/Creat RatioGlobulinGlucosePotassiumALT (Alanine Transaminase)

Total ProteinAlbumin/Globulin ratioChlorideSodiumSodium/Potassium Ratio

Collection Notes Please indicate method of urine collection. Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Submit sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: None

602 Antinuclear Antibody

Methodology: IFA

Specimen Requirements 0.5 mL Serum, SST/RTT. Minimum: 0.2 mL Serum. EDTA/heparinized plasma is OK.

Reported Time Tuesday/Thursday, Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: If positive, results will be held and a confirmatory titer will be run Wednesday/ Friday.Steroids will affect this test result.

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52 Autoimmune Panel

CBCPlatelet Count

Specimen Requirements ANA: 0.5 mL Serum, SST/RTTCBC and Coombs: 1.5 mL whole blood LTT(1.0 mL minimum) and air dried peripheral blood smears

Reported Time See Comment

Coombs ANA (Antinuclear Antibody)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Corticosteroids can cause false negative Coombs results, therefore consider discontinuing corticosteroid use 2-3 weeks prior to testing.

Additional Comments: Coombs performed Mon-Sat on Days. ANA is performed Tues/Thurs AM. A positive ANA will be held and is titered the next day (Wed/Fri)

Steroids will affect this test result, however, studies have shown that corticosteroid therapy of <7 days do not cause false negatives.

21 Basic Canine Chemistry

Methodology: Spectrophotometry

Chemistry ScreenCK (Creatine Kinase)

Specimen Requirements 0.5 mL Serum, SST/RTT, 1.0 mL GTT

Reported Time Same Day

Amylase Lipase

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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92 Basic Health Profile

CBCUrinalysis

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smears; Urinalysis: 5 mL fresh urine

Reported Time Same Day

Chemistry Screen Total T4

Collection Notes Please indicate method of urine collection.Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Submit sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: None

46 Bile Acids Panel

Methodology: Spectrophotometry

Bile acids, Pre

Specimen Requirements Pre and Post required, 0.3 mL (minimum 0.1 mL) serum each, SST/RTT.

Reported Time Same Day

Bile acids, Post

Collection Notes Pre Sample: 12 hour fasting samplePost Sample: 2 hour post prandial Use a standard meal such as maintenance or puppy formula food. Minimum recommendations = Feeding 2 tsp <10 lbs, 2 tbsp >10 lbs of canned, high quality food.PLEASE NOTE: The animal should be off Actigall (Ursodiol) 48 hours prior to running a bile acid study.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Post prandial values in normal dogs/cats do not usually exceed 20 umol/L. Bile acids testing is helpful in assessing liver function.GI motility abnormalities such as vomiting or diarrhea may affect results. If bilirubin is elevated then Bile acids are not necessary; they will be increased as well.

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104 Bile Acids, Single Sample

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum SST/RTT; Can test either Pre Sample (12 hour fasted sample) or Post sample (2 hour post prandial)

Reported Time Same Day

Collection Notes Use a standard meal such as maintenance or puppy formula food. Minimum recommendations = Feeding 2 tsp <10 lbs, 2 tbsp >10 lbs of canned, high quality food.Please Note: Animal should be off Actigall (Ursodiol 48 hours prior to running bile acids study.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This is a single sample evaluation, Pre OR Post sample. If BOTH Pre and Post are needed, order Bile Acids Panel 46. GI motility abnormalities such as vomiting or diarrhea may affect results. If bilirubin is elevated then Bile acids are not necessary; they will be increased as well.

610 Blood Typing, Canine

Methodology: RIM

Specimen Requirements 0.5 mL Whole Blood, LTT

Reported Time Daily AM - Same Day

Collection Notes None

Additional Comments: Types for DEA 1 only. Call the Lab for universal blood donor information.

201 Brucella Screen, Canine

Methodology: IFA

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Daily AM - Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Positive results should be confirmed by AGID. This is a send-out test to Cornell University (SO4 - Brucellosis Confirmation). Additional charges will apply. This will not be sent without client approval.

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1647 Canine Breeding Panel

Progesterone

Specimen Requirements Progesterone: 0.5 mL RTTCytology: 1-3 Vaginal Smears (Slides)

Reported Time Daily AM - Same Day

Cytology, Vaginal

Collection Notes Prepare the cytology sample by GENTLY rolling the swab over the slide. This technique will deliver optimal cellular material for review. Thoroughly dry the smears. DO NOT put slides into the slides holder until completely dry and do not refrigerate. Consistent labeling of tubes and smears: "Last Name, Owner, Animal, date" is very helpful when comparing serial results.Please allow all serum samples (RTT) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.Remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Timing of Sampling in Early Proestrus: 1) Progesterone should be monitored every 2-3 days to detect the preovulatory rise. 2) Vaginal Cytology should be evaluated every 2 days until >60% cornified. Rechecking: If progesterone is 1.0 - 3.0 ng/mL, consider confirming ovulation with serum progesterone concentration after at least 3 days. At ovulation, serum progesterone ranges from 3.8 - 10 ng/mL. Optimal breeding (fresh semen) is 2 days after ovulation.

1012 Canine GI Panel (cPL/TLI/Cobalamin/Folate)

Methodology: Chemiluminescent Immunoassay/Lateral Flow

CobalaminFolate

Specimen Requirements 1.0 mL Serum SST/RTT, hemolyzed samples should NOT be submitted for Folate testing.

Reported Time Mon, Wed, Fri, Same day

cPLI TLI

Collection Notes Fast animals 12-18 hours (diabetic animals 6-8 hours) before draw.Cover samples with foil, vet wrap, or a paper towel to protected from light (folate and cobalamin can be light sensitive) It is recommended to separate serum and freeze the sample if transport to lab is to be delayed.

Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: For Felines see Feline TLI/PLI/Cobalamin/Folate 1689.

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58 Canine Heartworm Panel

Methodology: ELISA/Knotts

Heartworm Antigen

Specimen Requirements 0.5 mL Serum, SST/RTT, and 1.0 mL Whole Blood, LTT(Test can be run on only LTT, volume 2.0 mL Whole Blood)

Reported Time Same Day

Microfilaria

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Testing recommended in dogs 7 months and older. Test sensitivity is limited to detection of adult female heartworm antigen. High worm burdens can have false negative antigen results. If the result is unexpected, consider re-testing with heat treatment.Positive antigen results verified by RIM test. This test is not recommended for use in cats as they usually have a low worm load (see Feline Heartworm Panel 1629).

To run only Heartworm antigen or microfilaria see: Heartworm Antigen 238 or Microfilaria 212.

156 Canine Immunization Screen

Methodology: IFA

Canine Distemper virus

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Monday, Thursday: Same Day

Parvovirus

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: For Vaccination status. Screens for antibodies to Canine Distemper virus and Parvovirus.

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1005 Canine PLI and TLI

Methodology: Chemiluminescent Immunoassay/Lateral Flow

Specimen Requirements 0.5 mL Serum SST/RTT For cPLI: Excessive hemolysis may obscure the results and sample could be rejected.

Reported Time Same Day/Next Day

Collection Notes Serum must be fasted 12-18 hours (diabetic animals 6-8 hours) and non-hemolyzed/non-lipemic.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments:

1013 Canine PLI/Cobalamin/Folate

Methodology: Chemiluminescent Immunoassay/Lateral Flow

cPLI

Specimen Requirements 1.0 mL Serum SST/RTT, hemolyzed samples should NOT be submitted for Folate testing.

Reported Time Mon, Wed, Fri, Same day

Cobalamin Folate

Collection Notes Fast animals 12-18 hours (diabetic animals 6-8 hours) before draw.Cover samples with foil, vet wrap, or a paper towel to protected from light (folate and cobalamin can be light sensitive) It is recommended to separate serum and freeze the sample if transport to lab is to be delayed.

Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: For Felines see Feline PLI/Cobalamin/Folate 4689.

44 CBC w/ Differential

Methodology: Flow Cytometry

Specimen Requirements 0.5 mL Whole Blood, LTT, and air dried peripheral blood smears

Reported Time Same Day

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: None

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73 CBC, Follow-Up

Methodology: Flow Cytometry

Specimen Requirements 1.0 mL Whole Blood, LTT. Air dried peripheral blood smears also recommended

Reported Time Same Day

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

1044 CBC, Platelet if Indicated

Methodology: Flow Cytometry

Specimen Requirements 0.5 mL Whole Blood, LTT

Reported Time Same Day

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: It is possible a platelet count cannot be performed due to clumping.

1 Chemistry Screen

Methodology: Spectrophotometry

GlucoseBUN (Blood Urea Nitrogen)CreatinineSodiumPotassiumSodium/Potassium RatioChlorideCarbon Dioxide (CO2)

Specimen Requirements 0.5 mL Serum, SST/RTTMinimum is 250uL serum

Reported Time Same Day

Anion GapCalciumPhosphorusTotal ProteinAlbuminGlobulinA/G Ratio

Bilirubin, TotalALP (Alkaline Phosphatase)ALT (Alanine Transaminase)AST (Aspartate Transaminase)GGT (Gamma-glutamyltransferase)CholesterolCalculated Osmolality

Collection Notes Prolonged contact with cells can dramatically affect glucose valuesFor accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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71 Chemistry Screen, Follow-up

Methodology: Spectrophotometry

GlucoseBUN (Blood Urea Nitrogen)CreatinineSodiumPotassiumSodium/Potassium RatioChloride

Specimen Requirements 0.5 mL Serum, SST/RTT, 1.0 mL GGT

Reported Time Same Day

CO2 (Carbon Dioxide)Anion GapCalciumPhosphorusTotal ProteinAlbuminGlobulinA/G Ratio

Bilirubin, TotalALP (Alkaline Phosphatase)ALT (Alanine Transaminase)AST (Aspartate Transaminase)GGT (Gamma-glutamyltransferase)CholesterolCalculated Osmolality

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

83 Coagulation Panel I

Methodology: Endpoint Clot Detection

Prothrombin Time (PT)

Specimen Requirements 1 BTT Citrated Plasma required, see collection note for volume details.

Reported Time Same Day

APTT

Collection Notes Citrated tubes (BTT) provided by Phoenix Laboratory hold 2.7 mL of blood (to the top of the label). Tubes must be filled with 2.7 mL of blood. If collecting a smaller volume of blood (<2.7mL) the blood to anticoagulant ratio should be 9:1. Please call lab with any questions.Protocol: 1. Draw fresh venous blood into BTT until full (to top of label) but not beyond the top of the tube label. No clotting should be observed in the tube. 2. Cool to 4° C (refrigeration) until analysis. 3. If a delay greater than 24 hours is expected prior to analysis, then the BTT should be spun in a centrifuge, the plasma separated and placed in a CTT or other non-additive tube to be frozen until analysis.

Additional Comments: The ratio of blood to anticoagulant is critical for valid coagulation results. Any clotting will results in the tube being rejected for analysis.

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10 Coagulation Panel II

CBC w/DifferentialPlatelet Count

Specimen Requirements 1 BTT, Centrifuge and Separate plasma (0.5mL minimum) and refrigerate. 1 LTT and 2 air-dried Blood smears

Reported Time Same Day

FibrinogenAPTT

Prothrombin Time (PT)

Collection Notes Citrated tubes (BTT) provided by Phoenix Laboratory hold 2.7 mL of blood (to the top of the label). Tubes must be filled with 2.7 mL of blood. If collecting a smaller volume of blood (<2.7mL) the blood to anticoagulant ratio should be 9:1. Please call lab with any questions.Protocol: 1. Draw fresh venous blood into BTT until full (to top of label) but not beyond the top of the tube label. No clotting should be observed in the tube. 2. Cool to 4° C (refrigeration) until analysis. 3. If a delay greater than 24 hours is expected prior to analysis, then the BTT should be spun in a centrifuge, the plasma separated and placed in a CTT or other non-additive tube to be frozen until analysis.

Additional Comments: The ratio of blood to anticoagulant is critical for valid coagulation results. Any clotting will results in the tube being rejected for analysis.Fibrinogen performed by heat precipitation.

18 Coagulation Panel III

CBCPlatelet Count

Specimen Requirements CBC and Fibrinogen: 1.5 mL LTT and air dried peripheral blood smearsCoagulation: 1 BTT, Centrifuge and separate plasma (0.5 mL minimum) and refrigerate.

Reported Time Same Day

D-DimerFibrinogen

Prothrombin Time (PT)APTT

Collection Notes Citrated tubes (BTT) provided by Phoenix Laboratory hold 2.7 mL of blood (to the top of the label). Tubes must be filled with 2.7 mL of blood. If collecting a smaller volume of blood (<2.7mL) the blood to anticoagulant ratio should be 9:1. Please call lab with any questions.Protocol: 1. Draw fresh venous blood into BTT until full (to top of label) but not beyond the top of the tube label. No clotting should be observed in the tube. 2. Cool to 4° C (refrigeration) until analysis. 3. If a delay greater than 24 hours is expected prior to analysis, then the BTT should be spun in a centrifuge, the plasma separated and placed in a CTT or other non-additive tube to be frozen until analysis.

Additional Comments: The ratio of blood to anticoagulant is critical for valid coagulation results. Any clotting will results in the tube being rejected for analysis.Fibrinogen performed by heat precipitation.

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70 Cobalamin (Vitamin B12)

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum, SST/RTT.

Reported Time Mon/Wed/Fri

Collection Notes Fast animals 12-18 hours (diabetic animals 6-8 hours) before draw.Cover sample with foil, vet wrap, or a paper towel to protected from light. sensitive).It is recommended to separate serum and freeze the sample if transport to lab is to be delayed.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Cobalamin is run in-house Monday, Wednesday, and Friday; reported out same day. Cobalamin results >1000 are re-run on dilution and reported out.

2689 Cobalamin and Folate

Methodology: Chemiluminescent Immunoassay

Specimen Requirements Serum must be fasted 12-18 hours (diabetic animals 6-8 hours). Sample must be non-hemolyzed for folate testing and avoid lipemic samples. Cover samples with foil, vet wrap, or a paper towel to protected from light (folate and cobalamin are light sensitive)

Reported Time Mon/Wed/Fri

Collection Notes Fast animals 12-18 hours (diabetic animals 6-8 hours) before draw.

Additional Comments: Cobalamin and Folate are run in-house Monday, Wednesday, and Friday; reported out same day. Folates >24.0 ug/dL and cobalamin >1000 are re-run on dilution and reported.

362 Coombs

Methodology: Direct Antiglobulin

Specimen Requirements 1.0 mL Whole Blood, LTT

Reported Time Daily, AM

Collection Notes Corticosteroids can cause false negative Coombs results if >7 days of therapy, therefore consider discontinuing corticosteroid use 2-3 weeks prior to testing.

Additional Comments: Steroids will affect this test result, however, studies have shown that corticosteroid therapy of <7 days do not cause false negatives.

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2231 Cortisol, Resting

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum, RTT

Reported Time Same Day

Collection Notes If the patient has been on steroids for >14 days, patient should be taperdown and discontinue for at least 2 weeks prior to testing. If <14 days, discontinue for 38 hours prior to testing.DO NOT USE SST TO COLLECT OR SPIN DOWN SAMPLE

Additional Comments: A patient with a resting cortisol >2.0 ug/dL has a 99% chance of NOT having Addison's disease.With a resting cortisol of <2.0 ug/dL, an ACTH Response 39 is needed to rule-in Addison's disease. This interpretation is valid if no oral or topical steroids have been given in the last 6 weeks. A resting cortisol cannot be used to rule- in or out hyperadrenocorticism. A LDDS 38 and/or ACTH Response 39 needs to be performed.

1000 cPL

Methodology: Lateral Flow

Specimen Requirements 0.25 mL Serum SST/RTT. Excessive hemolysis may obscure the results and sample could be rejected.

Reported Time Same Day AM/PM

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis. EDTA, sodium and lithium heparin sample tubes are acceptable.

Additional Comments: Semi-quantitative Canine Pancreatic Lipase Test. This test is specific for the determination of pancreas-specific lipase levels in canine serum or plasma.

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27 Crossmatch Complete

Methodology: Tube Agglutination

Crossmatch Major

Specimen Requirements 1.0 mL Serum, RTT and Whole Blood, LTT required for Recipient and for each donor tested. **Samples can not be hemolyzed.**

Reported Time Same Day

Crossmatch Minor

Collection Notes None

Additional Comments: Major: Determines if donor's RBCs are compatible with the recipient's serum.Minor: Determines if donor's plasma is compatible with the recipient's RBCs.A crossmatch is used prior to transfusion to determine if the blood to be transfused is compatible with the animal that will be receiving the transfusion. Individual charge for each tested donor. For Canine or feline only.

For only Major or Minor testing, see Crossmatch, Major 314, or Crossmatch, Minor 313.

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38 Dexamethasone Suppression

Methodology: Chemiluminescent Immunoassay

Pre Cortisol

Specimen Requirements 0.5 mL Serum each RTTx3; Pre, 4 hour, 8 hour post. DO NOT USE SST TO COLLECT OR SPIN DOWN SAMPLE

Reported Time Same Day

4 hr Post Cortisol 8 hr Post Cortisol

Collection Notes Collection Protocol:

Low Dose: NOTE: During the low dose dexamethasone suppression test, patient stress should be kept to a minimum. No other diagnostic procedures or grooming should be performed during the testing period.1. Collect 0.5 mL serum pre-injection. (RTT,CTT)2. Inject 0.01 mg/kg dexamethasone sodium phosphate IV.3. Collect 2 post samples at 3-4 and 8 hours post injection. (RTT,CTT) Centrifuge, separate serum into a sterile transport tube (RTT,CTT), and refrigerate or freeze until transport to the Lab.

High Dose:1. Collect 0.5 mL serum pre-injection. (RTT,CTT)2. Inject 0.1 mg/kg dexamethasone sodium phosphate IV.3. Collect 2 post samples at 4 and 8 hours post injection. Label each tube accordingly. (RTT,CTT) Centrifuge, separate serum into a sterile transport tube (RTT,CTT), and refrigerate or freeze until transport to the Lab. DO NOT FREEZE SPECIMENS IN GLASS TUBES.

Additional Comments: PLEASE NOTE: All three samples should be submitted together. A dexamethasone suppression is done to CONFIRM clinical suspicion of hyperadrenocorticism. Stressful diseases (ill diabetics, renal failure, etc.) and current drug therapy may cause false positives. Prednisone, prednisolone, and other corticosteroids (except dexamethasone) will cross react. Long term steroid therapy will suppress adrenal function. Recommend these drugs be discontinued at least 48 hrs before testing. Extra charge for more than 3 samples - please call the Laboratory for information. Up to 40% of pituitary dependent and adrenal dependent hyperadrenocorticoid dogs do not show suppression in LDDST. These dogs require HDDST, ACTH concentrations and/or abdominal imaging for further characterization.

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621 Distemper, FA

Methodology: FA

Specimen Requirements 1.0 mL Whole Blood LTT or conjunctival smears/swab or can run on both.

Reported Time Next Day

Collection Notes None

Additional Comments: May be positive for up to 3 weeks, post infection, in conjunctival smears; 2-5 days in whole blood.

Test set up next day, resulted by end of day.

16 Electrolytes Screen

Methodology: Spectrophotometry

SodiumPotassium

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

ChlorideCarbon Dioxide (CO2)

Anion GapSodium/Potassium Ratio

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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1008 Endocrine Panel

Chemistry Screen

Specimen Requirements 3 RTTs, Label each with draw time (0, 4, and 8 hour) 0.5 mL each ( minimum 0.1 mL for 4 and 8 hr posts).DO NOT USE SST TO COLLECT OR SPIN DOWN SAMPLE

Reported Time Same Day

Dexamethasone Suppression - 3 Cortisols

Collection Notes Collection Protocol: Low Dose: NOTE: During the low dose dexamethasone suppression test, patient stress should be kept to a minimum. No other diagnostic procedures or grooming should be performed during the testing period.1. Collect 0.5 mL serum pre-injection. (RTT,CTT)2. Inject 0.01 mg/kg dexamethasone, IV.3. Collect 2 post samples at 3-4 and 8 hours post injection. (RTT,CTT) Centrifuge, separate serum into a sterile transport tube (RTT,CTT), and refrigerate or freeze until transport to the Lab.

High Dose:1. Collect 0.5 mL serum pre-injection. (RTT,CTT)2. Inject 0.1 mg/kg dexamethasone, IV.3. Collect 2 post samples at 4 and 8 hours post injection. Label each tube accordingly. (RTT,CTT) Centrifuge, separate serum into a sterile transport tube (RTT,CTT), and refrigerate or freeze until transport to the Lab. DO NOT FREEZE SPECIMENS IN GLASS TUBES.

Additional Comments: PLEASE NOTE: All three samples should be submitted together. A dexamethasone suppression is done to CONFIRM clinical suspicion of hyperadrenocorticism. Stressful diseases (ill diabetics, renal failure, etc.) and current drug therapy may cause false positives. Prednisone, prednisolone, and other corticosteroids (except dexamethasone) will cross react. Long term steroid therapy will suppress adrenal function. Recommend these drugs be discontinued at least 48 hrs before testing. Extra charge for more than 3 samples - please call the Laboratory for information. Up to 40% of pituitary dependent and adrenal dependent hyperadrenocorticoid dogs do not show suppression in LDDST. These dogs require HDDST, ACTH concentrations and/or abdominal imaging for further characterization.

625 Ethylene Glycol

Methodology: Enzymatic

Specimen Requirements 0.5 mL Plasma, LTT/GrnTT

Reported Time Same Day

Collection Notes Separate from cells as soon as possible.

Additional Comments: Ethylene glycol levels peak 2 hours after ingestion in canines.

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2409 Fecal Parasite Combo w/ Giardia/Crypto IFA

Fecal Screen

Specimen Requirements 10 grams of Fresh Feces, Fecal Tube or bag. Sample can not contain clay litter.

Reported Time See Comment

Giardia/Crypto IFA

Collection Notes Do not collect sample contaminated with clay kitty litter. It causes interference with IFA testing. Wheat/corn litter OK.

Additional Comments: Fecal float includes sugar flotation with two centrifugation sequences; microscopic examination for ova and parasites, including Giardia cysts. IFA testing will detect the antigen of Giardia species and most Cryptosporidia.It will not detect some species of Cryptosporidium that are associated with birds and reptiles.

For avian/non-mammalian testing, order Avian/Non-Mammalian Giardia/Cryptosporidium 1530.

409 Fecal Parasite Screen

Methodology: Centrifugation/Flotation

Specimen Requirements 5-10 grams of Fresh Feces, Fecal Tube or bag. For most accurate results, please submit fresh feces (less than 24 hours old).

Reported Time Next day

Collection Notes None

Additional Comments: Test includes sugar flotation with two centrifugation sequences; microscopic examination for ova and parasites, including Giardia cysts. Giardia/Crypto IFA (241) is recommended to confirm the presence of Giardia as noted.

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69 Folate

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum, SST/RTT. Sample MUST be non-hemolyzed for folate testing and avoid lipemic samples.

Reported Time Mon/Wed/Fri, Same day

Collection Notes Fast animals 12-18 hours (diabetic animals 6-8 hours) before draw.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Cover samples with foil, vet wrap, or a paper towel to protected from light (folate can be light sensitive).It is recommended to separate serum and freeze the sample if transport to lab is to be delayed.

Additional Comments: Folate is run in-house Monday, Wednesday, and Friday; reported out same day. Folates >24.0 ug/dL are re-run on dilution and reported.

2247 Free T4

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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1247 Free T4,  Add-On

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Available as an add-on to an existing panel (within 7 days). Previous case ID is required to activate the discounted price.

2 General Diagnostic

CBCGlucoseBUN (Blood Urea Nitrogen)CreatinineSodiumPotassiumSodium/Potassium RatioChloride

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Carbon Dioxide (CO2)Anion GapCalciumPhosphorusTotal ProteinAlbuminGlobulinA/G Ratio

Bilirubin, TotalALP (Alkaline Phosphatase)ALT (Alanine Transaminase)AST (Aspartate Transaminase)GGT (Gamma-glutamyltransferase)CholesterolCalculated Osmolality

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: None

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77 General Diagnostic w/ T4

CBC

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Chemistry Screen Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

2072 General Diagnostic w/ Thyroid II

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Total T4 cTSH (Thyroid Stimulating Hormone)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: TSH only validated on canines, but has been used in felines.

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770 General Diagnostic w/ Triglycerides

CBC

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears.

Reported Time Same Day

Chemistry Screen Triglycerides

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: For triglycerides on body cavity fluid, see Body Cavity Fluid Triglycerides 2144

777 General Diagnostic w/T4 and Cobalamin/Folate

CBCChemistry Screen

Specimen Requirements Chemistry: 1.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears. Sample MUST be non-hemolyzed for folate testing and avoid lipemic samples.

Reported Time See additional comments Below

CobalaminFolate

Total T4

Collection Notes Patient must be fasted 12-18 hours (diabetic animals 6-8 hours). Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Cover samples with foil or a paper towel to protected from light (folate and cobalamin are light sensitive).Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistries and CBC run same day. Cobalamin and Folate are run in-house Monday, Wednesday, and Friday; reported out same day.Folates >24.0 ug/dL and cobalamin >1000 are re-run on dilution and reported.

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7711 General Diagnostic w/T4, Follow-Up

CBC

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Chemistry Screen Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

1283 General Diagnostic with Coagulation I

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsCoag: 1 BTT, See Collection Note for volume

Reported Time Same Day

PT (Prothrombin Time) APTT

Collection Notes Citrated tubes (BTT) provided by Phoenix Laboratory hold 2.7 mL of blood (to the top of the label). Tubes must be filled with 2.7 mL of blood. If collecting a smaller volume of blood (<2.7mL) the blood to anticoagulant ratio should be 9:1. Please call lab with any questions. Protocol: 1. Draw fresh venous blood into BTT until full (to top of label) but not beyond the top of the tube label. No clotting should be observed in the tube. 2. Cool to 4° C (refrigeration) until analysis. 3. If a delay greater than 24 hours is expected prior to analysis, then the BTT should be spun in a centrifuge, the plasma separated and placed in a CTT or other non-additive tube to be frozen until analysis.

For Chemistry: Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: The ratio of blood to anticoagulant is critical for valid coagulation results. Any clotting will results in the tube being rejected for analysis.

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72 General Diagnostic, Follow-Up

CBC

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Chemistry Screen

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

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61 General Endocrine, Canine

Chemistry Screen

Specimen Requirements 2 RTTs, Pre/Post 0.5 mL Serum Each, 1.0 mL GTTDO NOT USE SST TO SPIN DOWN SAMPLE

Reported Time Same Day

Total T4 ACTH Response (Pre and Post Cortisols)

Collection Notes A grey top tube is recommended but not necessary if the RTT is centrifuged and serum is transferred to a transport tube. PROTOCOL:1. Collect a resting sample in a RTT, 0.5 mL of serum is required. Separate serum if possible and refrigerate. 2. Inject 0.25 mg (250 µg) of Cortrosyn IM or IV (1 vial). 3. Collect a second sample EXACTLY 1 HOUR post injection in RTT, label clearly as to the collection status (post), and preserve in the same manner as the first sample. NOTE: The test has been validated using Cortrosyn. Although compounded gels are available, they vary greatly in peak times and are not recommended.

ALTERNATE CANINE PROTOCOL (Microdose): In order to keep Cortrosyn costs down, use the specific dose of 5 ug/kg IV - up to the maximum dose of 250 ug (one vial) per dog. Whatever is left over can then be split into aliquots of 25 ug or 50 ug using small plastic syringes. Normally 1 mL of sterile physiologic saline is used as diluent. If so, 25 ug would be 100 uL or 0.1 mL. ***When the patient is receiving Trilostane/Vetoryl, the timing of the test is important. The pre-cortisol sample should be drawn 3-5 hours after the morning capsule. The test is a one hour test, so in other words, the ACTH Response test should be completed 4-6 hours after the medication, which is usually given once a day in the morning.

Additional Comments: Pre and Post sample required. In addition to diagnosis of naturally occurring hyperadrenocorticism (Cushing's disease), this is the only diagnostic test for hypoadrenocorticism (Addison's disease), iatrogenic hyperadrenocorticism, and monitoring response to medical therapy of Cushing's disease. Prednisone, prednisolone, and other corticosteroids (except dexamethasone) will cross react. Recommend these drugs be discontinued at least 48 hrs before testing. If dog has been on prolonged steroids, patient must be tapered with the drug stopped for at least 3 weeks to let the adrenal gland recover. INTERPRETATION HYPERADRENOCORTICISM: An ACTH response test is done to CONFIRM clinical suspicion of hyperadrenocorticism. Stressful diseases (ill diabetics, renal failure, etc.) and current drug therapy may cause false positives. If the cortisol results are normal (and an adequate dose of Cortrosyn was administered) and there is high clinical suspicion of hyperadrenocorticism, sex hormone hyperadrenocorticism (atypical Cushing's) should be considered. Additional testing on the serum from this ACTH stimulation test can be performed. Please contact us within 7 days of this cortisol result for further testing options. A low-dose dexamethasone suppression test should also be considered, as some patients with Cushing's will be positive on one test but not on the other.INTERPRETATION HYPOADRENOCORTICISM: Pre and post cortisol <2 ug/dL are supportive of hypoadrenocorticism but need to be interpreted in light of other clinical findings and history. Failure to stimulate can occur with iatrogenic hyperadrenocorticism, dogs being treated for hyperadrenocorticism and Addison's disease.

For Feline Protocol See ACTH Response, Feline 1039

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2401 Giardia ELISA

Methodology: ELISA

Specimen Requirements 2 grams of Fresh Feces, Fecal Tube or bag.

Reported Time Same Day

Collection Notes None

Additional Comments: None

240 Giardia/Cryptosporidium IFA

Methodology: IFA

Specimen Requirements 3-5 grams of Fresh Feces, Fecal Tube or bag. Sample can not contain clay litter.

Reported Time Daily, AM

Collection Notes Do not collect sample contaminated with clay kitty litter. It causes interference with testing. Wheat/corn litter OK.

Additional Comments: IFA testing will detect the antigen of Giardia species and most Cryptosporidia. It will not detect some species of Cryptosporidium that are associated with birds and reptiles.

For avian/non-mammalian testing, see Avian/Non-Mammalian Giardia/Cryptosporidium 1530.

238 Heartworm Antigen

Methodology: ELISA

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Testing recommended in dogs 7 months and older. Test sensitivity is limited to detection of adult female heartworm antigen. Immature heartworms, including microfilaria, are not detected, see Microfilaria 212.High worm burdens can have false negative results. If the result is unexpected, consider re-testing with heat treatment.Positive results verified by RIM test. This test is not recommended for use in cats as they usually have a low worm load (see Heartworm Antibody 629).To combine with microfilaria, order Heartworm Panel 58.

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2380 Heartworm Antigen, Add-On

Methodology: ELISA

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Testing recommended in dogs 7 months and older. Test sensitivity is limited to detection of adult female heartworm antigen. Immature heartworms, including microfilaria, are not detected, see Microfilaria 212.High worm burdens can have false negative results. If the result is unexpected, consider re-testing with heat treatment.Positive results verified by RIM test. This test is not recommended for use in cats as they usually have a low worm load (see Heartworm Antibody 629).To combine with microfilaria, order Heartworm Panel 58.Available as an add-on to an existing panel (within 7 days). Previous case ID is required to activate the discounted price.

632 IgA Quantitative, Canine

Methodology: Radial Immunodiffusion (RID)

Specimen Requirements 0.3 mL Serum, SST/RTT

Reported Time 48 Hours

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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13 Immune Mediated Hemolytic Anemia (IMHA) Panel

CBCCoombs

Specimen Requirements 1.0 mL Whole Blood LTT and air dried peripheral blood smears

Reported Time Same Day

Platelet Count Reticulocyte Count

Collection Notes Corticosteroids can cause false negative Coombs results if >7 days of therapy, therefore consider discontinuing corticosteroid use 2-3 weeks prior to testing.

Additional Comments: Coombs run on day shift only.Steroids will affect this test result, however, studies have shown that corticosteroid therapy of <7 days do not cause false negatives.

634 Insulin

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 1.0 mL Serum, Minimum: 0.5 mL, SST/RTT, Frozen**Serum can NOT be hemolyzed as hemolysis will interfere with testing.**

Reported Time Mon, Wed, Fri, Same day

Collection Notes Centrifuge tube within 20-30 minutes of collection. Separate serum into a transport tube. Sample should be tested or frozen within 24 hours. If unable to centrifuge, use GTT for collection of glucose samples. GTT tubes must have a minimum of 1 mL of blood for accurate results.

Additional Comments: In combination with clinical signs and concurrent blood glucose concentration, determination of serum insulin may be useful in diagnosing an insulinoma. In assessing whether the serum insulin concentration is appropriate, it must be compared to the serum glucose concentration (taken concurrently; included in the cost of insulin test). Measurement of serum insulin concentrations is most useful when the blood glucose concentration is 59 mg/dL or less.Equine insulin and glucose levels have been suggested for diagnosing pituitary adenoma/Cushing's disease, but can be abnormal in any disease causing insulin resistance. Testing not recommended on samples >24 hours old due to rapid deterioration.

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1718 Juvenile Routine Screen

CBCAlbuminALP (Alkaline Phosphatase)BUN (Blood Urea Nitrogen)Creatinine

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

BUN/Creat RatioGlobulinPotassiumALT (Alanine Transaminase)Total Protein

Albumin/Globulin ratioChlorideSodiumSodium/Potassium RatioGlucose

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Does not include a urinalysis.

385 Leptospirosis Rapid Screen

Methodology: RIM

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Leptospirosis is a zoonotic disease, transmitted by contact of the organism with mucous membranes. Take extra caution when handling animals/samples suspected of infection.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Test includes, but can not distinguish between, serovars Canicola, Grippotyphosa, Icterohaemorrhagiae, and Pomona. For additional Leptospirosis testing including MAT and PCR testing , see our Sendout Catalog.

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1084 Liver Chemistry Panel

Methodology: Spectrophotometry

AlbuminALP (Alkaline Phosphatase)ALT (Alanine Transaminase)AST (Aspartate Transaminase)

Specimen Requirements Chemistry: Two SST/RTT samples, Pre and Post, 0.5 mL each

Reported Time Same Day

Total ProteinGlobulinA/G RatioPre Bile AcidsPost Bile Acids

Bilirubin, DirectBilirubin, IndirectBilirubin, TotalGGT (Gamma-glutamyltransferase)

Collection Notes Pre Sample: 12 hr fastingPost: 2 hr post prandial.Use a standard meal such as maintenance or puppy formula food. Minimum recommendations = Feeding 2 tsp <10 lbs, 2 tbsp >10 lbs of canned, high quality food.PLEASE NOTE: The animal should be off Actigall (Ursodiol) 48 hours prior to running a bile acid study.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Post prandial values in normal dogs/cats do not usually exceed 20 umol/L. Bile acids testing is used to assess liver function.

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85 Liver Profile

CBCAlbuminALP (Alkaline Phosphatase)ALT (Alanine Transaminase)AST (Aspartate Transaminase)

Specimen Requirements Chemistry: Two SST/RTT samples, Pre and Post, 0.5 mL eachCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Total ProteinGlobulinA/G RatioPre Bile Acids SamplePost Bile Acids Sample

Bilirubin DirectBilirubin IndirectBilirubin TotalGGT (Gamma-glutamyltransferase)

Collection Notes Pre Sample: 12 hour fasting samplePost Sample: 2 hour post prandialUse a standard meal such as maintenance or puppy formula food. Minimum recommendations = Feeding 2 tsp <10 lbs, 2 tbsp >10 lbs of canned, high quality food.PLEASE NOTE: The animal should be off Actigall (Ursodiol) 48 hours prior to running a bile acid study.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Post prandial values in normal dogs/cats do not usually exceed 20 umol/L. Bile acids testing is used to assess liver function.

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620 Luteinizing Hormone

Methodology: RIM

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Please allow sample to clot (up to 20 minutes) and then spin down sample. If using a RTT, remove serum and put into a fresh RTT/CTT. Avoid lipemia and hemolysis.

Additional Comments: OVARIAN REMNANT DETERMINATION: Test when the dog is NOT showing signs of estrus: A positive result means high LH concentrations, indicating no ovarian tissue. (Occasionally false positives may occur with the 24 hr LH surge.) A negative result means low LH concentrations, indicating the presence of ovarian tissue. Exposure to estrogen creams will also cause a negative LH result.OVULATION TIMING IN THE BITCH: The duration of the LH peak varies from dog to dog and lasts only 24 hours in approximately 40% of the bitches. For this reason, serum should be tested for LH every day beginning on the 4th or 5th day of proestrus or when vaginal cytology approaches 50% cornification. When a positive result is obtained this is considered "Day 0". A progesterone test should be done on Day 0 and Day 3 to confirm a rise in progesterone. If the detected increase in LH was the actual preovulatory surge, progesterone concentrations should increase above 2 ng/mL within 3 days and stay elevated. If the progesterone remains low, less than 2 ng/mL, it indicates that a proestrus fluctuation in LH was identified. Continue daily testing until the true LH surge occurs. Days 4 through 7 post LH surge encompass the true fertile period with peak fertility on Days 5 and 6. Breeding on Days 2, 4, and 6 post LH surge will completely cover the fertile period and maximize viable sperm numbers on the bitch's most fertile days. Indeterminant results may indicate a negative result or that the LH surge is approaching or recently peaked. For animals with unknown ovarian status, this result may indicate that the animal has been ovariectomized. NOTE: This test has not been validated for, but has been used in, cats with similar interpretations as in dogs.

212 Microfilaria

Methodology: Modified Knotts

Specimen Requirements 1.0 mL Whole Blood, LTT

Reported Time Same Day

Collection Notes None

Additional Comments: Recommend testing only in dogs 7 months of age and older. Microfilaria are rarely detected in cats.Test included in Heartworm Panels, see Heartworm panel 58 or Feline Heartworm Panel 1629.

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8502 Oncology Profile

CBCGlucoseBUN (Blood Urea Nitrogen)

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same day

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)AlbuminCalcium

CreatinineTotal ProteinGlobulin

Collection Notes Please allow sample to clot (up to 20 minutes) and then spin down sample. If using a RTT, remove serum and put into a fresh RTT/CTT. Avoid lipemia and hemolysis.

A grey top tube is recommended but not necessary if the SST submitted has complete separation after centrifugation, or the RTT is centrifuged and serum is transferred to a transport tube.

Additional Comments: This test is for monitoring effects of chemotherapy.

213 Ova & Parasite

Methodology: Centrifugation/Microscopic

Specimen Requirements 5 grams Fresh Feces, Fecal Tube or bag

Reported Time Next day

Collection Notes None

Additional Comments: Test includes a direct smear as well as a sugar flotation with two centrifugation sequences; microscopic examination for ova and parasites, including Giardia cysts. Giardia/Crypto IFA add-on #241 is recommended to confirm the presence of Giardia as noted.

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2213 Ova & Parasite Combo w/IFA

Ova & Parasite

Specimen Requirements 10 grams of Fresh Feces, Fecal Tube or bag. Sample can not contain clay litter.

Reported Time Next Day

Giardia/Crypto IFA

Collection Notes Do not collect sample contaminated with clay kitty litter. It causes interference with IFA testing. Wheat/corn litter OK.

Additional Comments: Ova and parasite test includes a direct smear as well as a sugar flotation with two centrifugation sequences; microscopic examination for ova and parasites, including Giardia cysts. IFA testing will detect the antigen of Giardia species and most Cryptosporidia. It will not detect some species of Cryptosporidium that are associated with birds and reptiles.

For avian/non-mammalian testing, see Avian/Non-Mammalian Giardia/Cryptosporidium 1530.

215 Parvovirus Antibody, Serum

Methodology: IFA

Specimen Requirements 0.25 mL Serum, SST/RTT

Reported Time Daily, AM

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Used to detect parvovirus antibody, differentiating between IgG and IgM. Can also be used to evaluate for adequate titer post vaccine.

214 Parvovirus Antigen, Fecal

Methodology: RIM

Specimen Requirements 1 grams Fresh Feces, Fecal Tube or bag

Reported Time Same Day

Collection Notes None

Additional Comments: Used to evaluate for clinical infection in dogs only. Virus is shed for only a short-time.

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216 Phenobarbital

Methodology: Spectrophotometry

Specimen Requirements 0.5 mL Serum, RTT, peak OR trough sample

Reported Time Same Day

Collection Notes Drawn as single sample, trough or peak. Trough levels usually are most helpful. Trough:  Just prior to next dose   Peak: 4-6 hours post dose Protocol: 1. Select the desired dosing regimen and administer the drug until steady state is reached (2 weeks after change in dose). 2. Collect the sample as trough (preferably just prior to next dose) or peak (preferably 4-6 hours post dose)Please allow all serum samples (RTT) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.Remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: In dogs with well-controlled seizures, after steady state is reached (2 weeks after a change in dose), trough levels of phenobarbital should be monitored periodically. Ideally, these samples should be collected as a fasting sample prior to the next dose (i.e. if the dog receives phenobarbital at 8am and 8pm, the blood should be collected at 8am or 8pm, prior to phenobarbital administration). When toxicity is suspected, (or sometimes in animals with poorly controlled seizures), trough and peak serum phenobarbital concentration determination may be helpful, see Pheonbarbital, Peak and Trough 79.The time of peak blood phenobarbital concentrations is variable; usually a sample collected 6 hours after phenobarbital administration may be considered adequate for peak serum phenobarbital concentration.The half-life of phenobarbital varies widely among individual animals, therefore monitoring of serum levels aids in establishing an appropriate dose. Phenobarbital concentrations are also useful for evaluating owner compliance. Phenobarbital is the active component of primidone.

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79 Phenobarbital, Peak & Trough

Methodology: Spectrophotometry

Phenobarbital, Peak

Specimen Requirements 0.5 mL Serum, 2 RTT, sample Label accordingly (Peak/Trough)

Reported Time Same Day

Phenobarbital, Trough

Collection Notes Protocol:1. Select the desired dosing regimen and administer the drug until steady state is reached (2 weeks after change in dose).2. Collect the trough sample (preferably just prior to next dose) and collect the peak sample (preferably 6 hours post dose).

Please allow all serum samples (RTT) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.Remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: In dogs with well-controlled seizures, after steady state is reached (2 weeks after a change in dose), trough levels of phenobarbital should be monitored periodically. Ideally, these samples should be collected as a fasting sample prior to the next dose (i.e. if the dog receives phenobarbital at 8am and 8pm, the blood should be collected at 8am or 8pm, prior to phenobarbital administration). When toxicity is suspected, (or sometimes in animals with poorly controlled seizures), trough and peak serum phenobarbital concentration determination may be helpful. The time of peak blood phenobarbital concentrations is variable; usually a sample collected 6 hours after phenobarbital administration may be considered adequate for peak serum phenobarbital concentration.The half-life of phenobarbital varies widely among individual animals, therefore monitoring of serum levels aids in establishing an appropriate dose. Phenobarbital concentrations are also useful for evaluating owner compliance. Phenobarbital is the active component of primidone.

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850 Pre-Op Panel

CBCGlucoseBUN (Blood Urea Nitrogen)

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

CreatinineTotal ProteinGlobulin

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)Albumin

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: None

1851 Pre-Op Panel, Follow-up

CBCGlucoseBUN (Blood Urea Nitrogen)

Specimen Requirements Chemistry: 0.5 mL Serum,SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

CreatinineTotal ProteinAlbumin

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)Globulin

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

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547 Progesterone, Canine

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum, RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.Remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Canine: To best predict optimal breeding dates, serial progesterone results should be interpreted in conjunction with Vaginal Cytology (Canine Breeding Panel, Test 1647). At ovulation, serum progesterone concentration ranges from 3.8-10 ng/mL. Optimal breeding (fresh semen) is 2 days after ovulation (if frozen semen: 4 days after ovulation). For timing of whelping, progesterone decreases to <2 ng/mL 36-48 hours prior to whelping. After the initial test, follow-up testing is offered; order Progesterone Follow-up, Canine 747.

This test code is for canine use only. All other species use Progesterone test code #647

747 Progesterone, Follow-Up

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum, RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.Remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This is to be used for canine prebreeding or prewhelping if multiple testing is needed.

Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

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31 Protein Electrophoresis

Agarose Gel Electrophoresis

Specimen Requirements 0.25 mL Serum, SST/RTT. Can be run on heparinized plasma for avians/nonmammalians.

Reported Time Monday and Thursday, Same Day

Collection Notes None

Additional Comments: Test can also be performed on body cavity effusions to assist in diagnosis of FIP.

300 Relaxin, Canine or Feline

Methodology: RIM

Specimen Requirements 0.5 mL Serum, SST/RTT.Test can also be run on heparinized or EDTA Plasma (GrnTT/LTT).Markedly hemolyzed or lipemic samples should not be used.

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Day 20 of gestation is found to be the earliest day of pregnancy detection. Relaxin rises after implantation of a fertilized egg. Implantation occurs 26-31 days after the LH surge. Any negative result is considered indicative of non-pregnancy; however, two negative results one week apart may be required for confirmation of this status, especially when date of mating is unknown. A positive result is considered indicative of pregnancy. A weak positive result may be observed at the beginning of the pregnancy. It is recommended to repeat the test on a fresh sample one week later to confirm pregnancy.

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1724 Relocated Canine Routine Screen

CBCUrinalysisVector/Tick Borne Disease ScreenAlbuminGlobulinAlb/Glob Ratio

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urine

Reported Time Same day

GlucoseBUN ( Blood Urea Nitrogen)CreatinineBUN/Crea RatioTotal proteinHeartworm Panel (in 6 months)

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)SodiumPotassiumChloride

Collection Notes Please indicate method of urine collection.Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Use code 17246 and include original accession number when submitting the 6 month follow up Canine Heartworm Panel. Follow Up testing must be submitted within 12 months.

5023 Renal Panel, Chronic

Methodology: Spectrophotometry

BUN (Blood Urea Nitrogen)Creatinine

Specimen Requirements 0.3 mL Serum, SST/RTT. Please call our Client Services for information about minimums on short samples.

Reported Time Same Day

Phosphorus Potassium

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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5025 Renal Profile

HCT (Hematocrit)Creatinine

Specimen Requirements 0.5 mL of each, Serum, SST/RTT0.1 mL Whole Blood, LTT.A sealed crit w/ blood also accepted in lieu of LTT.

Reported Time Same Day

PotassiumCalcium

PhosphorusBUN (Blood Urea Nitrogen)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Run every 3 to 6 months depending on clinical signs.

5027 Renal Profile w/ Urinalysis

UrinalysisHCT (Hematocrit)BUN (Blood Urea Nitrogen)

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTTUrinalysis: 5 mL fresh urine, Urine Tube/CTT

Reported Time Same Day

CreatininePhosphorus

PotassiumCalcium

Collection Notes Please indicate method of urine collection.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Run every 3 to 6 months depending on clinic signs.

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2231 Resting Cortisol

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum, RTT

Reported Time Same Day

Collection Notes If the patient has been on steroids for >14 days, patient should be taperdown and discontinue for at least 2 weeks prior to testing. If <14 days, discontinue for 38 hours prior to testing.DO NOT USE SST TO COLLECT OR SPIN DOWN SAMPLE

Additional Comments: A patient with a resting cortisol >2.0 ug/dL has a 99% chance of NOT having Addison's disease.With a resting cortisol of <2.0 ug/dL, an ACTH Response 39 is needed to rule-in Addison's disease. This interpretation is valid if no oral or topical steroids have been given in the last 6 weeks. A resting cortisol cannot be used to rule- in or out hyperadrenocorticism. A LDDS 38 and/or ACTH Response 39 needs to be performed.

1716 Senior Routine Screen

CBCGlucoseBUN (Blood Urea Nitrogen)CreatinineBUN/Creat RatioSodiumPotassium

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urine

Reported Time Same Day

Sodium/Potassium RatioChlorideCalciumPhosphorusTotal ProteinAlbuminGlobulin

Albumin/Globulin ratioBilirubin TotalALP (Alkaline Phosphatase)ALT (Alanine Transaminase)Total T4Urinalysis

Collection Notes Please indicate method of urine collection. Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: None

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11 Senior Screen

Methodology: Spectrophotometry

GlucosePotassiumPhosphorusChloride

Specimen Requirements 0.5 mL Serum SST/RTT

Reported Time Same Day

BUN (Blood Urea Nitrogen)CreatinineNa/K RatioTotal T4

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)Sodium

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

For a Senior Screen w/ CBC, order 899

899 Senior Screen w/ CBC

CBCGlucosePotassiumSodium

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

PhosphorusBUN (Blood Urea Nitrogen)CreatinineChloride

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)Na/K RatioTotal T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

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2071 Senior Screen W/ TSH

GlucosePotassiumSodiumPhosphorus

Specimen Requirements 0.5 mL Serum, SST/RTT, 1 mL GTT

Reported Time Same Day

BUN (Blood Urea Nitrogen)CreatinineChlorideTotal T4

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)Na/K RatioTSH (Thyroid Stimulating Hormone)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: TSH only validated on canines, but has been used in felines. Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

1101 Senior Screen w/Cholesterol

Methodology: Spectrophotometry

GlucosePotassiumSodiumNa/K Ratio

Specimen Requirements 0.5 mL Serum SST/RTT, 1.0 mL GTT

Reported Time Same Day

BUN (Blood Urea Nitrogen)CreatinineTotal T4Chloride

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)CholesterolPhosphorus

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

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651 T3 (Triiodothyronine)

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Mondays, Same day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Test is run every Monday.

74 Thyroid I

Methodology: Chemiluminescent Immunoassay

Free T4

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Next day

TSH (Thyroid Stimulating Hormone)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: TSH only validated on canines, but has been used in felines.

75 Thyroid II

Total T4

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

TSH (Thyroid Stimulating Hormone)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: TSH only validated on canines, but has been used in felines. Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

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864 Thyroid III

TSH (Thyroid Stimulating Hormone)

Specimen Requirements 1.0 mL Serum, SST/RTT

Reported Time Same Day

Total T4 Free T4

Collection Notes Peak samples are drawn 4-6 hours post pill Trough samples are drawn just before the next dose.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: TSH only validated on canines, but has been used in felines.Feline T4: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

874 Thyroid IV

Total T4

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

Free T4

Collection Notes Peak samples are drawn 4-6 hours post pill. Trough samples are drawn just before the next dose.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

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49 Thyroid Screen

Total T4

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time See Comment

T3 (Triiodothyronine)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: T4 run same day, T3 run every Monday. Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

68 TLI, Canine

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum, SST/RTT.

Reported Time Mon/Wed/Fri, Same day

Collection Notes Patient must be fasted 12-18 hours (diabetic animals 6-8 hours).We strongly encourage that all serum samples be allowed to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Used to diagnose exocrine pancreatic insuffiency (EPI).

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689C TLI/Cobalamin/Folate (CANINE ONLY)

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 1.0 mL Serum, SST/RTT. Sample MUST be non-hemolyzed for folate testing and avoid lipemic samples.

Reported Time Mon/Wed/Fri, Same day

Collection Notes Patient must be fasted 12-18 hours (diabetic animals 6-8 hours).Cover samples with foil, vet wrap, or a paper towel to protected from light (folate and cobalamin can be light sensitive). It is recommended to separate serum and freeze the sample if transport to lab is to be delayed.We strongly encourage that all serum samples be allowed to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Tests are run in-house Monday, Wednesday, and Friday; reported out same day.Folates >24.0 ug/dL and Cobalamin >1000 are re-run on dilution and reported.

3 Total Body Diagnostic

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urine

Reported Time Same Day

AmylaseLipase

CK (Creatine Kinase)Urinalysis

Collection Notes Please indicate method of urine collection. Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: None

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35 Total Body Diagnostic w/o Urinalysis

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

LipaseAmylase

CK (Creatine Kinase)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: If you would like this panel and a Urinalysis, See Total Body Diagnostic 3

1035 Total Body Diagnostic w/o Urinalysis, Follow Up

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

AmylaseLipase

CK (Creatine Kinase)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

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1003 Total Body Diagnostic, Follow-Up

CBCChemistry Screen

Specimen Requirements Chemistry: SST/RTT Minimum: 0.5 mL serum CBC: 2 air dried slides, 1.0mL LTT Urinalysis: 5mL urine

Reported Time Same Day

AmylaseLipase

CK (Creatine Kinase)Urinalysis

Collection Notes Please indicate method of urine collection.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

248 TSH (Thyroid Stimulation Hormone)

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum, SST/RTT. Minimum: 0.2 mL Serum.

Reported Time Same Day

Collection Notes None

Additional Comments: Run on canines (for confirmation of hypothyroidism) and felines (for confirmation of hyperthyroidism.)

TSH only validated on canines, but has been used in felines.

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159 Urinalysis

Specimen Requirements 5 mL Urine, Urine Tube/CTT

Reported Time Same Day

Collection Notes Please indicate method of collection.Submit sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Includes: Urine specific gravity run on analyzer, verified by refractometer, Chemistries (pH, Glucose, Protein, Ketones, Blood, and Bilirubin) run on Urine analyzer, and microscopic examination of sediment. Low volume samples will use dipstick readings. Low volumes may also be changed to Urine Sediment Only 155.

548 Urinalysis w/ C&S

Urinalysis

Specimen Requirements 5 mL Urine, Urine Tube/CTT. For the most accurate results 1 mL urine in sterile container should be submitted in addition to 5 mL urine.

Reported Time See Comment

Urine Culture

Collection Notes Please indicate method of urine collection.Do not submit syringes with needles, they are not accepted. Whenever possible, urine should be collected by cystocentesis. When this is not possible, free-catch urine into sterile container.Submit sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Turn around time is same day for the UA and 36 - 72 hours for Culture. Culture includes sensitivity panels for 2 pathogens. Additional sensitivities can be requested for an additional charge. No growth cultures are finalized after 48 hours of incubation. Urine collected on a swab or thio will not allow a colony count to be provided.

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552 Urinalysis w/ Culture, Follow-up

Urinalysis

Specimen Requirements 5 mL Urine, Urine Tube/CTT. For the most accurate results 1 mL urine in sterile container should be submitted in addition to 5 mL urine.

Reported Time See Comments

Urine Culture

Collection Notes Please indicate method of urine collection.Do not submit syringes with needles, they are not accepted. Whenever possible, urine should be collected by cystocentesis. When this is not possible, free-catch urine into sterile container.Submit sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Please include all antibiotic history with urine culture.Turn around time is same day for the UA and 36 - 72 hours for Culture. Culture includes sensitivity panels for 2 pathogens. Additional sensitivities can be requested for an additional charge. No growth cultures are finalized after 48 hours of incubation. Urine collected on a swab or thio will not allow a colony count to be provided.

Submit within 30 days of original test. Previous Case ID is required to activate the discounted price

234 Urine Cortisol/Creatinine Ratio

Urine Cortisol

Specimen Requirements 0.5 mL Urine, Urine Tube/CTTIf sample is bloody or very cloudy/cellular, submit a larger volume (1.0 mL minimum) as the sample will need to be centrifuged.

Reported Time Same Day

Urine Creatinine Ucor/Ucreat Ratio

Collection Notes If the patient has been on steroids for >14 days, discontinue for 2 weeks prior to testing. If <14 days, discontinue for 48 hours prior to testing.

Additional Comments: INTERPRETATION:An elevated urine cortisol/creatinine ratio (> 20) provides additional support for canine hyperadrenocorticism but is not specific for that condition. Many disorders cause an increased result. A normal result (<20) makes hyperadrenocorticism very unlikely. Further testing (low dose Dexamethasone Suppression Test and/or ACTH Stimulation Test) is advised to establish a diagnosis of hyperadrenocorticism.

*Note that both cortisol and creatinine have been converted to moles to get this ratio.

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551 Urine Culture, Follow-up

Methodology: Culture

Specimen Requirements 1.0 mL Urine, CTTCollected via cystocentesis preferred

Reported Time 36-72 hours

Collection Notes Whenever possible, urine should be collected by cystocentesis. If not possible, collect catheterized or free catch urine sample into sterile container. Refrigerate urine sample after collection.

Additional Comments: Please include all antibiotic history with urine culture.Colony counts are not possible if a plate, broth, or swab is submitted. Culture includes sensitivities on up to 2 isolates. Cultures are quantitated and reported in CFU/mL. No growth cultures are finalized after 48 hours of incubation.

Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.To include with a urinalysis, refer to Urinalysis with Culture, Follow-up 552.

1031 Urine Protein Electrophoresis

Methodology: Agarose Gel Electrophoresis

Specimen Requirements 5 mL Urine, CTT/Urine Tube

Reported Time Monday/Thursday

Collection Notes None

Additional Comments: Please submit one day before testing to allow for urine concentration.

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387 Vector/Tick Borne Disease Screen

Methodology: Lateral Flow

Specimen Requirements 0.5 mL Serum, SST/RTT. Plasma or whole blood (EDTA or heparinized) may be used.

Reported Time Daily AM - Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Test includes: Ehrlichia canis, Ehrlichia ewirgii, Ehrlichia chaffeensis, Anaplasma phagocytophilum, Anaplasma platys, Borrelia burgdorferi (Lyme Disease), and Heartworm (D. immitis).

Test methodology unable to differentiate between Anaplasma phagocytophilum and Anaplasma platys. Also unable to differentiate between Ehrlichia canis, Ehrlichia chaffeensis, and Ehrlichia ewingii.

Also see Canine Tick-borne Disease panel, SO666 if screening for Rocky Mountain Spotted Fever is desired.

1711 Wellness Screen II

CBCChemistry ScreenUrinalysis

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urine

Reported Time Same Day

AmylaseLipase

CK (Creatine Kinase)Total T4

Collection Notes Please indicate method of urine collection. Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: To request this panel, but submit the urinalysis portion later, see Wellness Screen II w/ Urine to Follow 1711NU

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1721 Wellness Screen II w/ SDMA

CBCChemistry Screen Urinalysis

Specimen Requirements Chemistry: 1.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urine

Reported Time See comment

AmylaseLipaseSDMA

CK (Creatine Kinase)Total T4

Collection Notes Please indicate method of collection.Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: CBC, Chemistries, and urinalysis run same day, SDMA Results Next Day. See SDMA SO513 for more details about test.

1712 Wellness Screen III

CBCChemistry ScreenFree T4

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same day

AmylaseLipase

CK (Creatine Kinase)Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Panel includes Wellness Screen I and Free T4. Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

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1713 Wellness Screen IV

CBCChemistry ScreenUrinalysis

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urine

Reported Time Same day

AmylaseLipaseFree T4

CK (Creatine Kinase)Total T4

Collection Notes Please indicate method of urine collection. Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

15 Wellness/Geriatric Panel

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

AmylaseLipase

CK (Creatine Kinase)Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: None

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1722 Wellness/Geriatric Panel w/ SDMA

CBCChemistry ScreenSDMA

Specimen Requirements Chemistry: 1.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time See comment

AmylaseLipase

CK (Creatine Kinase)Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: CBC and Chemistries run same day, SDMA results next day. See SDMA SO513 for more details about test.

1160 Wellness/Geriatric Panel w/Triglycerides

CBCChemistry ScreenTriglycerides

Specimen Requirements Chemistry: 0.5 mL serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT, air dried peripheral blood smears

Reported Time Same Day

AmylaseLipase

CK (Creatine Kinase)Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: None

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1015 Wellness/Geriatric Panel, Follow-Up

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT, air dried peripheral blood smears

Reported Time Same Day

AmylaseLipase

CK (Creatine Kinase)Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

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Feline Panels

1039 ACTH Response, Feline

Methodology: Chemiluminescent Immunoassay

Pre Cortisol

Specimen Requirements 3 RTT Minimum:  0.25 mL for each sampleAvoid severe lipemia

Reported Time Same Day

30 min Post Cortisol 60 min Post Cortisol

Collection Notes FELINE PROTOCOL: DO NOT USE SST TO COLLECT OR SPIN DOWN SAMPLE1.  Collect a resting sample in a RTT, 0.5 mL of serum is required.  Separate serum if possible and refrigerate. 2.  Inject 0.125 mg  of Cortrosyn  IM or IV, (1/2 vial). 3.  Collect a sample exactly 30 min and 60 min post injection in a RTT, label clearly as to the status (post-30, post-60), and preserve in the same manner as the first sample.

Additional Comments: Feline hyperadrenocorticism is very rare, we suggest consulting with an internist/clinical pathologist at the Laboratory prior to diagnostic testing. Dexamethasone suppression test is the favored diagnostic test for hyperadrenocorticism in cats. ACTH Response test is not recommended.NOTE: The test has been validated using Cortrosyn. Although compounded gels are available, they vary greatly in peak times and are not recommended. Prednisone, prednisolone, and other corticosteroids (except dexamethasone) will cross react. Recommend these drugs be discontinued at least 48 hrs before testing. If the patient has been on steroids >14 days, patient must be tapered and discontinue steroids for at least two weeks prior to testing.

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1719 Adult Routine Screen

CBCAlbuminALP (Alkaline Phosphatase)BUN (Blood Urea Nitrogen)CreatinineUrinalysis

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urine

Reported Time Same Day

BUN/Creat RatioGlobulinGlucosePotassiumALT (Alanine Transaminase)

Total ProteinAlbumin/Globulin ratioChlorideSodiumSodium/Potassium Ratio

Collection Notes Please indicate method of urine collection. Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Submit sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: None

602 Antinuclear Antibody

Methodology: IFA

Specimen Requirements 0.5 mL Serum, SST/RTT. Minimum: 0.2 mL Serum. EDTA/heparinized plasma is OK.

Reported Time Tuesday/Thursday, Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: If positive, results will be held and a confirmatory titer will be run Wednesday/ Friday.Steroids will affect this test result.

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52 Autoimmune Panel

CBCPlatelet Count

Specimen Requirements ANA: 0.5 mL Serum, SST/RTTCBC and Coombs: 1.5 mL whole blood LTT(1.0 mL minimum) and air dried peripheral blood smears

Reported Time See Comment

Coombs ANA (Antinuclear Antibody)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Corticosteroids can cause false negative Coombs results, therefore consider discontinuing corticosteroid use 2-3 weeks prior to testing.

Additional Comments: Coombs performed Mon-Sat on Days. ANA is performed Tues/Thurs AM. A positive ANA will be held and is titered the next day (Wed/Fri)

Steroids will affect this test result, however, studies have shown that corticosteroid therapy of <7 days do not cause false negatives.

57 Basic Feline

CBCChemistry Screen

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time See Comment

CK (Creatine Kinase)Total T4

FeLV (ELISA)FIV (IFA)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistries, CBC and FELV done same day.IFA testing done next day.In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

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92 Basic Health Profile

CBCUrinalysis

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smears; Urinalysis: 5 mL fresh urine

Reported Time Same Day

Chemistry Screen Total T4

Collection Notes Please indicate method of urine collection.Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Submit sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: None

104 Bile Acids, Single Sample

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum SST/RTT; Can test either Pre Sample (12 hour fasted sample) or Post sample (2 hour post prandial)

Reported Time Same Day

Collection Notes Use a standard meal such as maintenance or puppy formula food. Minimum recommendations = Feeding 2 tsp <10 lbs, 2 tbsp >10 lbs of canned, high quality food.Please Note: Animal should be off Actigall (Ursodiol 48 hours prior to running bile acids study.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This is a single sample evaluation, Pre OR Post sample. If BOTH Pre and Post are needed, order Bile Acids Panel 46. GI motility abnormalities such as vomiting or diarrhea may affect results. If bilirubin is elevated then Bile acids are not necessary; they will be increased as well.

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611 Blood Typing, Feline

Methodology: Agglutination

Specimen Requirements 0.5 mL Whole Blood, LTT

Reported Time Daily AM - Same Day

Collection Notes None

Additional Comments: Test consists of two antigens: A and B. Test cannot be performed on samples that exhibit auto-agglutination. Cats with type B erythrocytes will suffer an immediate and severe anaphylactic reaction when transfused with type A blood.

44 CBC w/ Differential

Methodology: Flow Cytometry

Specimen Requirements 0.5 mL Whole Blood, LTT, and air dried peripheral blood smears

Reported Time Same Day

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: None

73 CBC, Follow-Up

Methodology: Flow Cytometry

Specimen Requirements 1.0 mL Whole Blood, LTT. Air dried peripheral blood smears also recommended

Reported Time Same Day

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

1044 CBC, Platelet if Indicated

Methodology: Flow Cytometry

Specimen Requirements 0.5 mL Whole Blood, LTT

Reported Time Same Day

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: It is possible a platelet count cannot be performed due to clumping.

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1 Chemistry Screen

Methodology: Spectrophotometry

GlucoseBUN (Blood Urea Nitrogen)CreatinineSodiumPotassiumSodium/Potassium RatioChlorideCarbon Dioxide (CO2)

Specimen Requirements 0.5 mL Serum, SST/RTTMinimum is 250uL serum

Reported Time Same Day

Anion GapCalciumPhosphorusTotal ProteinAlbuminGlobulinA/G Ratio

Bilirubin, TotalALP (Alkaline Phosphatase)ALT (Alanine Transaminase)AST (Aspartate Transaminase)GGT (Gamma-glutamyltransferase)CholesterolCalculated Osmolality

Collection Notes Prolonged contact with cells can dramatically affect glucose valuesFor accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

853 Chemistry Screen w/ T4 and Feline Viral II

Chemistry ScreenTotal T4

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time See Comment

FeLV (ELISA)FIV (IFA)

FCoV (FIP) Screen (IFA)

Test Alias: Special Feline w/Chemistry Screen

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistry, and FeLV run same day, FIV/FIP run daily, AM. Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted. Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

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71 Chemistry Screen, Follow-up

Methodology: Spectrophotometry

GlucoseBUN (Blood Urea Nitrogen)CreatinineSodiumPotassiumSodium/Potassium RatioChloride

Specimen Requirements 0.5 mL Serum, SST/RTT, 1.0 mL GGT

Reported Time Same Day

CO2 (Carbon Dioxide)Anion GapCalciumPhosphorusTotal ProteinAlbuminGlobulinA/G Ratio

Bilirubin, TotalALP (Alkaline Phosphatase)ALT (Alanine Transaminase)AST (Aspartate Transaminase)GGT (Gamma-glutamyltransferase)CholesterolCalculated Osmolality

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

83 Coagulation Panel I

Methodology: Endpoint Clot Detection

Prothrombin Time (PT)

Specimen Requirements 1 BTT Citrated Plasma required, see collection note for volume details.

Reported Time Same Day

APTT

Collection Notes Citrated tubes (BTT) provided by Phoenix Laboratory hold 2.7 mL of blood (to the top of the label). Tubes must be filled with 2.7 mL of blood. If collecting a smaller volume of blood (<2.7mL) the blood to anticoagulant ratio should be 9:1. Please call lab with any questions.Protocol: 1. Draw fresh venous blood into BTT until full (to top of label) but not beyond the top of the tube label. No clotting should be observed in the tube. 2. Cool to 4° C (refrigeration) until analysis. 3. If a delay greater than 24 hours is expected prior to analysis, then the BTT should be spun in a centrifuge, the plasma separated and placed in a CTT or other non-additive tube to be frozen until analysis.

Additional Comments: The ratio of blood to anticoagulant is critical for valid coagulation results. Any clotting will results in the tube being rejected for analysis.

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10 Coagulation Panel II

CBC w/DifferentialPlatelet Count

Specimen Requirements 1 BTT, Centrifuge and Separate plasma (0.5mL minimum) and refrigerate. 1 LTT and 2 air-dried Blood smears

Reported Time Same Day

FibrinogenAPTT

Prothrombin Time (PT)

Collection Notes Citrated tubes (BTT) provided by Phoenix Laboratory hold 2.7 mL of blood (to the top of the label). Tubes must be filled with 2.7 mL of blood. If collecting a smaller volume of blood (<2.7mL) the blood to anticoagulant ratio should be 9:1. Please call lab with any questions.Protocol: 1. Draw fresh venous blood into BTT until full (to top of label) but not beyond the top of the tube label. No clotting should be observed in the tube. 2. Cool to 4° C (refrigeration) until analysis. 3. If a delay greater than 24 hours is expected prior to analysis, then the BTT should be spun in a centrifuge, the plasma separated and placed in a CTT or other non-additive tube to be frozen until analysis.

Additional Comments: The ratio of blood to anticoagulant is critical for valid coagulation results. Any clotting will results in the tube being rejected for analysis.Fibrinogen performed by heat precipitation.

18 Coagulation Panel III

CBCPlatelet Count

Specimen Requirements CBC and Fibrinogen: 1.5 mL LTT and air dried peripheral blood smearsCoagulation: 1 BTT, Centrifuge and separate plasma (0.5 mL minimum) and refrigerate.

Reported Time Same Day

D-DimerFibrinogen

Prothrombin Time (PT)APTT

Collection Notes Citrated tubes (BTT) provided by Phoenix Laboratory hold 2.7 mL of blood (to the top of the label). Tubes must be filled with 2.7 mL of blood. If collecting a smaller volume of blood (<2.7mL) the blood to anticoagulant ratio should be 9:1. Please call lab with any questions.Protocol: 1. Draw fresh venous blood into BTT until full (to top of label) but not beyond the top of the tube label. No clotting should be observed in the tube. 2. Cool to 4° C (refrigeration) until analysis. 3. If a delay greater than 24 hours is expected prior to analysis, then the BTT should be spun in a centrifuge, the plasma separated and placed in a CTT or other non-additive tube to be frozen until analysis.

Additional Comments: The ratio of blood to anticoagulant is critical for valid coagulation results. Any clotting will results in the tube being rejected for analysis.Fibrinogen performed by heat precipitation.

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70 Cobalamin (Vitamin B12)

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum, SST/RTT.

Reported Time Mon/Wed/Fri

Collection Notes Fast animals 12-18 hours (diabetic animals 6-8 hours) before draw.Cover sample with foil, vet wrap, or a paper towel to protected from light. sensitive).It is recommended to separate serum and freeze the sample if transport to lab is to be delayed.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Cobalamin is run in-house Monday, Wednesday, and Friday; reported out same day. Cobalamin results >1000 are re-run on dilution and reported out.

2689 Cobalamin and Folate

Methodology: Chemiluminescent Immunoassay

Specimen Requirements Serum must be fasted 12-18 hours (diabetic animals 6-8 hours). Sample must be non-hemolyzed for folate testing and avoid lipemic samples. Cover samples with foil, vet wrap, or a paper towel to protected from light (folate and cobalamin are light sensitive)

Reported Time Mon/Wed/Fri

Collection Notes Fast animals 12-18 hours (diabetic animals 6-8 hours) before draw.

Additional Comments: Cobalamin and Folate are run in-house Monday, Wednesday, and Friday; reported out same day. Folates >24.0 ug/dL and cobalamin >1000 are re-run on dilution and reported.

362 Coombs

Methodology: Direct Antiglobulin

Specimen Requirements 1.0 mL Whole Blood, LTT

Reported Time Daily, AM

Collection Notes Corticosteroids can cause false negative Coombs results if >7 days of therapy, therefore consider discontinuing corticosteroid use 2-3 weeks prior to testing.

Additional Comments: Steroids will affect this test result, however, studies have shown that corticosteroid therapy of <7 days do not cause false negatives.

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2231 Cortisol, Resting

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum, RTT

Reported Time Same Day

Collection Notes If the patient has been on steroids for >14 days, patient should be taperdown and discontinue for at least 2 weeks prior to testing. If <14 days, discontinue for 38 hours prior to testing.DO NOT USE SST TO COLLECT OR SPIN DOWN SAMPLE

Additional Comments: A patient with a resting cortisol >2.0 ug/dL has a 99% chance of NOT having Addison's disease.With a resting cortisol of <2.0 ug/dL, an ACTH Response 39 is needed to rule-in Addison's disease. This interpretation is valid if no oral or topical steroids have been given in the last 6 weeks. A resting cortisol cannot be used to rule- in or out hyperadrenocorticism. A LDDS 38 and/or ACTH Response 39 needs to be performed.

27 Crossmatch Complete

Methodology: Tube Agglutination

Crossmatch Major

Specimen Requirements 1.0 mL Serum, RTT and Whole Blood, LTT required for Recipient and for each donor tested. **Samples can not be hemolyzed.**

Reported Time Same Day

Crossmatch Minor

Collection Notes None

Additional Comments: Major: Determines if donor's RBCs are compatible with the recipient's serum.Minor: Determines if donor's plasma is compatible with the recipient's RBCs.A crossmatch is used prior to transfusion to determine if the blood to be transfused is compatible with the animal that will be receiving the transfusion. Individual charge for each tested donor. For Canine or feline only.

For only Major or Minor testing, see Crossmatch, Major 314, or Crossmatch, Minor 313.

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16 Electrolytes Screen

Methodology: Spectrophotometry

SodiumPotassium

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

ChlorideCarbon Dioxide (CO2)

Anion GapSodium/Potassium Ratio

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

625 Ethylene Glycol

Methodology: Enzymatic

Specimen Requirements 0.5 mL Plasma, LTT/GrnTT

Reported Time Same Day

Collection Notes Separate from cells as soon as possible.

Additional Comments: Ethylene glycol levels peak 2 hours after ingestion in canines.

2409 Fecal Parasite Combo w/ Giardia/Crypto IFA

Fecal Screen

Specimen Requirements 10 grams of Fresh Feces, Fecal Tube or bag. Sample can not contain clay litter.

Reported Time See Comment

Giardia/Crypto IFA

Collection Notes Do not collect sample contaminated with clay kitty litter. It causes interference with IFA testing. Wheat/corn litter OK.

Additional Comments: Fecal float includes sugar flotation with two centrifugation sequences; microscopic examination for ova and parasites, including Giardia cysts. IFA testing will detect the antigen of Giardia species and most Cryptosporidia.It will not detect some species of Cryptosporidium that are associated with birds and reptiles.

For avian/non-mammalian testing, order Avian/Non-Mammalian Giardia/Cryptosporidium 1530.

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409 Fecal Parasite Screen

Methodology: Centrifugation/Flotation

Specimen Requirements 5-10 grams of Fresh Feces, Fecal Tube or bag. For most accurate results, please submit fresh feces (less than 24 hours old).

Reported Time Next day

Collection Notes None

Additional Comments: Test includes sugar flotation with two centrifugation sequences; microscopic examination for ova and parasites, including Giardia cysts. Giardia/Crypto IFA (241) is recommended to confirm the presence of Giardia as noted.

1720 Feline ADR Panel

CBCChemistry ScreenUrinalysis

Specimen Requirements Chemistry: 1.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urineO&P/Gardia/Crypto: 5 grams fresh feces

Reported Time See Comment

FIPFeLVFIV

Ova and ParasiteGiardia/Crypto IFAFecal Culture

Collection Notes Please indicate method of urine collection. Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.For most accurate results, please submit fresh feces (less than 24 hours old). Do not collect sample contaminated with clay kitty litter. It causes interference with testing. Wheat/Corn litter OK.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: O&P: Test includes sugar flotation with two centrifugation sequences; microscopic examination for ova and parasites, including Giardia cysts. Giardia/Crypto IFA testing will detect the antigen of Giardia species and most Cryptosporidia. Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)Not recommended testing for juveniles. CBC, Chemistries, urinalysis, and FeLV run same day. FIP/FIV performed Mon - Sat AM, reported in afternoon. Fecal Culture reported in 36 - 72 hours; Fecal culture for the detection of Salmonella and Campylobacter. Shigella and Yersinia can be ruled out upon request. Additional charges may apply.

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1612 Feline Calicivirus Titer

Methodology: IFA

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Monday, Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This test is for checking immunization status.

209 Feline Coronavirus (FIP) Screen

Methodology: IFA

Specimen Requirements 0.5 mL Serum, SST/RTT. Test can also be run on abdominal fluid.

Reported Time Daily, AM

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This test is NOT specific for FIP. Testing performed at dilutions of 1:100 and 1:6400. For titered results see Coronavirus (FIP) titer 217.

Test can also be run on abdominal fluid, however, Body Cavity Fluid Cytology (#14) with Feline Coronavirus Antigen FA (SO529) recommended.

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249 Feline Coronavirus (FIP) Screen, Add-On

Methodology: IFA

Specimen Requirements 0.5 mL Serum, SST/RTT. Minimum: 0.1 mL Serum.

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This test is NOT specific for FIP. Testing performed at dilutions of 1:100 and 1:6400.For titered results see Coronavirus (FIP) titer 217.

Test can also be run on abdominal fluid, however, Body Cavity Fluid Cytology (#14) with Feline Coronavirus Antigen FA (SO529) recommended.

Available as an add-on to an existing panel (within 7 days). Previous case ID is required to activate the discounted price.

217 Feline Coronavirus (FIP), Titered

Methodology: IFA

Specimen Requirements 0.5 mL Serum, SST/RTT. Test can also be run on abdominal fluid.

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This is a quantitative titer testing method. It is NOT specific for FIP. Test involves eight serial dilutions ranging from 1:100 to 1:25,600.

Test can also be run on abdominal fluid, however, Body Cavity Fluid Cytology (#14) with Feline Coronavirus Antigen FA (SO529) recommended.

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1629 Feline Heartworm Panel

Feline Heartworm Antibody

Specimen Requirements 0.5 mL Serum, SST/RTT and 1.0 mL Whole Blood LTT

Reported Time Same Day

Microfilaria (Knott's Test)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This test includes Feline Antibody test and microfilaria. Microfilaria are rarely detected in cats.For Feline heartworm antibody and antigen see Feline Heartworm Screen 2629

2629 Feline Heartworm Screen

Methodology: RIM/ELISA

Feline Heartworm Antibody

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

Heartworm Antigen

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This test includes heartworm antibody and antigen and is recommended by the American Heartworm Society (www.heartwormsociety.com).

For Feline Antibody test and microfilaria order Feline Heartworm Panel 1629.

1611 Feline Herpesvirus Titer

Methodology: IFA

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Monday, Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This test is for checking immunization status.

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161 Feline Immunization Screen I

Methodology: IFA

Feline Calicivirus

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Monday, Same Day

Feline Herpesvirus

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This screen is for checking immunization status. This screen is limited to feline calici and herpes virus. Also available are Immunization Screen II 1161, which screens for feline panleukopenia and herpes virus, and Immunization Screen III 2161, which screens for all three viruses.

1161 Feline Immunization Screen II

Methodology: IFA

Feline Panleukopenia virus

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Monday, Same Day

Feline Herpesvirus

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This test is run every Monday with results out that same day. This screen is limited to feline panleukopenia and herpes virus.Also available are Immunization Screen I 161 which tests for feline calici and herpes virus, and Immunization Screen III 2161 which screens for all three viruses.Titer <1:50 recommend vaccination.

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2161 Feline Immunization Screen III

Methodology: IFA

Feline Panleukopenia virus

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Monday, Same Day

Feline Herpesvirus Feline Calicivirus

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This screen is inclusive of Feline Immunization Screens I 161 and II 1161.Limited to feline panleukopenia, herpes, and calici viruses.

859 Feline Panel

CBCChemistry Screen

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time See Comments

CK (Creatine Kinase)Total T4

FeLV (ELISA)FIV (IFA)FCoV (FIP) Screen (IFA)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistry, CBC and FeLV run same day. FIV/FIP run daily, AM. FIV Recommend repeat in 6-8 weeks, if result is weak positive. Vaccinated cats will have a positive antibody response indistinguishable from that of infected cats for at least one year post vaccination.

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1613 Feline Panleukopenia Virus Titer

Methodology: IFA

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Monday, Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This test is for checking immunization status.

4689 Feline PLI/Cobalamin/Folate

fPLI (Spec fPL™)

Specimen Requirements 1.5 mL Serum, SST/RTT. Sample MUST be non-hemolyzed for folate testing and avoid lipemic samples.

Reported Time Mon, Wed, Fri

Cobalamin Folate

Collection Notes Patient must be fasted 12-18 hours (diabetic animals 6-8 hours).Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Cover samples with foil, vet wrap, or a paper towel to protected from light (folate and cobalamin are light sensitive)

Additional Comments: Cobalamin and Folate are run in-house Monday, Wednesday, and Friday; reported out same day. fPLI (Spec fPL™) are shipped to Steiner GI Laboratory, Texas A&M University Monday, Wednesday, Thursday; turn around time is 4-7 days.

For canines order Canine PLI/Cobalamin/Folate 1013.

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5 Feline Screen

Chemistry Screen

Specimen Requirements Chemistry: 0.75 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

CBC FeLV (ELISA)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: None

1689 Feline TLI/PLI/Cobalamin/Folate

TLIfPLI (Spec fPL™)

Specimen Requirements 1.5 mL Serum, SST/RTT. Sample MUST be non-hemolyzed for folate testing and avoid lipemic samples.

Reported Time See comment

Cobalamin Folate

Collection Notes Patient must be fasted 12-18 hours (diabetic animals 6-8 hours).Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Cover samples with foil, vet wrap, or a paper towel to protected from light (folate and cobalamin can be light sensitive).

Additional Comments: Cobalamin and Folate are run in-house Monday, Wednesday, and Friday; reported out same day. TLI and fPLI (Spec fPL™) are shipped to Steiner GI Laboratory, Texas A&M University Monday, Wednesday, Thursday; turn around time is 4-7 days.For Canines see Canine TLI/PLI/Cobalamin/Folate 1012.

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1696 Feline Toxoplasmosis IgG/IgM

Methodology: IFA

Specimen Requirements 0.2 mL Serum, SST/RTT

Reported Time Run M,W,F on days

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This test code is for felines only. Positive titers will be repeated andreported on the next run day

For other species see: Canine Toxoplasmosis IgG/IgM SO696 or Toxoplasmosis IgG/IgM, Other SO495

865 Feline Viral I

FeLV (ELISA)

Specimen Requirements 0.3 mL Serum, SST/RTT.EDTA or Heparinized Plasma OK.

Reported Time See Comment

FIV (IFA)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: FeLV run same dayFIV run Daily, AM.Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

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78 Feline Viral II

FeLV (ELISA)

Specimen Requirements 0.5 mL Serum, SST/RTT. EDTA or Heparinized Plasma OK.

Reported Time See Comments

FIV (IFA) FCoV (FIP) Screen (IFA)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: FeLV run same day. FIP/FIV performed Mon - Sat AM, reported in afternoon. This test is NOT specific for FIP. Testing performed at dilutions of 1:100 and 1:6400. For titered results see Coronavirus (FIP) titer 217Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

860 Feline Viral III

FeLV (ELISA)FIV (IFA)

Specimen Requirements 1.0 mL Serum, SST/RTT and 0.5 mL LTT and/or air dried peripheral blood smears

Reported Time See Comments

FIP (FCoV) Screen (IFA) Mycoplasma hemofelis (Hemobartonella) (Smear Evaluation)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: FeLV and Mycoplasma hemofelis (Hemobartonella) run same day.FIV and FIP set up daily, AM. This test is NOT specific for FIP. Testing performed at dilutions of 1:100 and 1:6400. For titered results see Coronavirus (FIP) titer 217.Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)Mycoplasma hemofelis (Hemobartonella) test is a smear evaluation that will not diagnose occult infections. Consider Mycoplasma hemofelis (Hemobartonella felis) PCR testing SO1638 as clinically indicated.

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321 FeLV (ELISA)

Methodology: ELISA

Specimen Requirements 0.5 mL Serum, SST/RTT. Can be performed on EDTA or heparinized Plasma.

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

361 FeLV, Add-On

Methodology: ELISA

Specimen Requirements 0.5 mL Serum, SST/RTTCan be performed on EDTA or heparinized Plasma.

Reported Time Same Day

Collection Notes None

Additional Comments: Available as an add-on to an existing panel (within 7 days). Previous case ID is required to activate the discounted price.

668 FIV

Methodology: IFA

Specimen Requirements 0.5 mL Serum, SST/RTT. Minimum: 0.1 mL serum.EDTA or heparinized plasma OK.

Reported Time Daily, Same Day

Collection Notes Please allow sample to clot (up to 20 minutes) and then spin down sample. If using a RTT, remove serum and put into a fresh RTT/CTT. Avoid lipemia and hemolysis.

Additional Comments: Positive results will be held for additional testing and will be resulted next day. Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

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768 FIV, Add-On

Methodology: IFA

Specimen Requirements 0.3 mL Serum, SST/RTT. EDTA or heparinized plasma OK.

Reported Time Same Day

Collection Notes None

Additional Comments: Positive results will be held for additional testing and will be resulted next day. Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

Available as add-on to existing panel (within 7 days). Previous case ID is required to activate the discounted price.

69 Folate

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum, SST/RTT. Sample MUST be non-hemolyzed for folate testing and avoid lipemic samples.

Reported Time Mon/Wed/Fri, Same day

Collection Notes Fast animals 12-18 hours (diabetic animals 6-8 hours) before draw.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Cover samples with foil, vet wrap, or a paper towel to protected from light (folate can be light sensitive).It is recommended to separate serum and freeze the sample if transport to lab is to be delayed.

Additional Comments: Folate is run in-house Monday, Wednesday, and Friday; reported out same day. Folates >24.0 ug/dL are re-run on dilution and reported.

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2247 Free T4

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

2 General Diagnostic

CBCGlucoseBUN (Blood Urea Nitrogen)CreatinineSodiumPotassiumSodium/Potassium RatioChloride

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Carbon Dioxide (CO2)Anion GapCalciumPhosphorusTotal ProteinAlbuminGlobulinA/G Ratio

Bilirubin, TotalALP (Alkaline Phosphatase)ALT (Alanine Transaminase)AST (Aspartate Transaminase)GGT (Gamma-glutamyltransferase)CholesterolCalculated Osmolality

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: None

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857 General Diagnostic w/ Feline Viral I

CBCChemistry Screen

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time See Comment

FeLV (ELISA) FIV (IFA)

Test Alias: Feline Screen w/FIV

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistry, CBC and FeLV run same day. FIV run daily, AM. Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

856 General Diagnostic W/ Feline Viral II

CBCChemistry Screen

Specimen Requirements Chemistry: 1.0 mL Serum,SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time See Comment

FeLV (ELISA)FIV (IFA)

FCoV (FIP) Screen (IFA)

Test Alias: Special Feline w/General Diag, (no Total T4)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistry, CBC and FeLV run same day. FIV/FIP run daily, AM.Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

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77 General Diagnostic w/ T4

CBC

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Chemistry Screen Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

8571 General Diagnostic w/ T4 and Feline Viral I

CBCChemistry Screen

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time See Comment

Total T4FeLV (ELISA)

FIV (IFA)

Test Alias: General Diag w/T4+FELV/FIV

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistry, CBC and FeLV run same day. FIV run daily, AM.Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

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855 General Diagnostic w/ T4 and Feline Viral II

CBCChemistry Screen

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time See Comments

Total T4FeLV (ELISA)

FCoV (FIP) Screen (IFA)FIV (IFA)

Test Alias: Special Feline w/General Diagnostic, T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistry, CBC, and FeLV run same day, FIV/FIP run daily, AM.

Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted. Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

2077 General Diagnostic w/ T4 and FeLV

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Total T4 FeLV

Test Alias: Spec Feline 77 w/FeLV

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

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770 General Diagnostic w/ Triglycerides

CBC

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears.

Reported Time Same Day

Chemistry Screen Triglycerides

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: For triglycerides on body cavity fluid, see Body Cavity Fluid Triglycerides 2144

777 General Diagnostic w/T4 and Cobalamin/Folate

CBCChemistry Screen

Specimen Requirements Chemistry: 1.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears. Sample MUST be non-hemolyzed for folate testing and avoid lipemic samples.

Reported Time See additional comments Below

CobalaminFolate

Total T4

Collection Notes Patient must be fasted 12-18 hours (diabetic animals 6-8 hours). Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Cover samples with foil or a paper towel to protected from light (folate and cobalamin are light sensitive).Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistries and CBC run same day. Cobalamin and Folate are run in-house Monday, Wednesday, and Friday; reported out same day.Folates >24.0 ug/dL and cobalamin >1000 are re-run on dilution and reported.

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7711 General Diagnostic w/T4, Follow-Up

CBC

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Chemistry Screen Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

1283 General Diagnostic with Coagulation I

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsCoag: 1 BTT, See Collection Note for volume

Reported Time Same Day

PT (Prothrombin Time) APTT

Collection Notes Citrated tubes (BTT) provided by Phoenix Laboratory hold 2.7 mL of blood (to the top of the label). Tubes must be filled with 2.7 mL of blood. If collecting a smaller volume of blood (<2.7mL) the blood to anticoagulant ratio should be 9:1. Please call lab with any questions. Protocol: 1. Draw fresh venous blood into BTT until full (to top of label) but not beyond the top of the tube label. No clotting should be observed in the tube. 2. Cool to 4° C (refrigeration) until analysis. 3. If a delay greater than 24 hours is expected prior to analysis, then the BTT should be spun in a centrifuge, the plasma separated and placed in a CTT or other non-additive tube to be frozen until analysis.

For Chemistry: Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: The ratio of blood to anticoagulant is critical for valid coagulation results. Any clotting will results in the tube being rejected for analysis.

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72 General Diagnostic, Follow-Up

CBC

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Chemistry Screen

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

2401 Giardia ELISA

Methodology: ELISA

Specimen Requirements 2 grams of Fresh Feces, Fecal Tube or bag.

Reported Time Same Day

Collection Notes None

Additional Comments: None

240 Giardia/Cryptosporidium IFA

Methodology: IFA

Specimen Requirements 3-5 grams of Fresh Feces, Fecal Tube or bag. Sample can not contain clay litter.

Reported Time Daily, AM

Collection Notes Do not collect sample contaminated with clay kitty litter. It causes interference with testing. Wheat/corn litter OK.

Additional Comments: IFA testing will detect the antigen of Giardia species and most Cryptosporidia. It will not detect some species of Cryptosporidium that are associated with birds and reptiles.

For avian/non-mammalian testing, see Avian/Non-Mammalian Giardia/Cryptosporidium 1530.

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629 Heartworm Antibody, Feline

Methodology: RIM

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Infections in felines are often single-sex worms in small numbers (1-3 worms). Antibodies are first detected in some cats at 50 -60 days. This test is the recommended Heartworm test for cats.To combine with Microfilaria, please order Feline Heartworm Panel #1629

13 Immune Mediated Hemolytic Anemia (IMHA) Panel

CBCCoombs

Specimen Requirements 1.0 mL Whole Blood LTT and air dried peripheral blood smears

Reported Time Same Day

Platelet Count Reticulocyte Count

Collection Notes Corticosteroids can cause false negative Coombs results if >7 days of therapy, therefore consider discontinuing corticosteroid use 2-3 weeks prior to testing.

Additional Comments: Coombs run on day shift only.Steroids will affect this test result, however, studies have shown that corticosteroid therapy of <7 days do not cause false negatives.

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1718 Juvenile Routine Screen

CBCAlbuminALP (Alkaline Phosphatase)BUN (Blood Urea Nitrogen)Creatinine

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

BUN/Creat RatioGlobulinPotassiumALT (Alanine Transaminase)Total Protein

Albumin/Globulin ratioChlorideSodiumSodium/Potassium RatioGlucose

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Does not include a urinalysis.

1084 Liver Chemistry Panel

Methodology: Spectrophotometry

AlbuminALP (Alkaline Phosphatase)ALT (Alanine Transaminase)AST (Aspartate Transaminase)

Specimen Requirements Chemistry: Two SST/RTT samples, Pre and Post, 0.5 mL each

Reported Time Same Day

Total ProteinGlobulinA/G RatioPre Bile AcidsPost Bile Acids

Bilirubin, DirectBilirubin, IndirectBilirubin, TotalGGT (Gamma-glutamyltransferase)

Collection Notes Pre Sample: 12 hr fastingPost: 2 hr post prandial.Use a standard meal such as maintenance or puppy formula food. Minimum recommendations = Feeding 2 tsp <10 lbs, 2 tbsp >10 lbs of canned, high quality food.PLEASE NOTE: The animal should be off Actigall (Ursodiol) 48 hours prior to running a bile acid study.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Post prandial values in normal dogs/cats do not usually exceed 20 umol/L. Bile acids testing is used to assess liver function.

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85 Liver Profile

CBCAlbuminALP (Alkaline Phosphatase)ALT (Alanine Transaminase)AST (Aspartate Transaminase)

Specimen Requirements Chemistry: Two SST/RTT samples, Pre and Post, 0.5 mL eachCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Total ProteinGlobulinA/G RatioPre Bile Acids SamplePost Bile Acids Sample

Bilirubin DirectBilirubin IndirectBilirubin TotalGGT (Gamma-glutamyltransferase)

Collection Notes Pre Sample: 12 hour fasting samplePost Sample: 2 hour post prandialUse a standard meal such as maintenance or puppy formula food. Minimum recommendations = Feeding 2 tsp <10 lbs, 2 tbsp >10 lbs of canned, high quality food.PLEASE NOTE: The animal should be off Actigall (Ursodiol) 48 hours prior to running a bile acid study.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Post prandial values in normal dogs/cats do not usually exceed 20 umol/L. Bile acids testing is used to assess liver function.

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620 Luteinizing Hormone

Methodology: RIM

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Please allow sample to clot (up to 20 minutes) and then spin down sample. If using a RTT, remove serum and put into a fresh RTT/CTT. Avoid lipemia and hemolysis.

Additional Comments: OVARIAN REMNANT DETERMINATION: Test when the dog is NOT showing signs of estrus: A positive result means high LH concentrations, indicating no ovarian tissue. (Occasionally false positives may occur with the 24 hr LH surge.) A negative result means low LH concentrations, indicating the presence of ovarian tissue. Exposure to estrogen creams will also cause a negative LH result.OVULATION TIMING IN THE BITCH: The duration of the LH peak varies from dog to dog and lasts only 24 hours in approximately 40% of the bitches. For this reason, serum should be tested for LH every day beginning on the 4th or 5th day of proestrus or when vaginal cytology approaches 50% cornification. When a positive result is obtained this is considered "Day 0". A progesterone test should be done on Day 0 and Day 3 to confirm a rise in progesterone. If the detected increase in LH was the actual preovulatory surge, progesterone concentrations should increase above 2 ng/mL within 3 days and stay elevated. If the progesterone remains low, less than 2 ng/mL, it indicates that a proestrus fluctuation in LH was identified. Continue daily testing until the true LH surge occurs. Days 4 through 7 post LH surge encompass the true fertile period with peak fertility on Days 5 and 6. Breeding on Days 2, 4, and 6 post LH surge will completely cover the fertile period and maximize viable sperm numbers on the bitch's most fertile days. Indeterminant results may indicate a negative result or that the LH surge is approaching or recently peaked. For animals with unknown ovarian status, this result may indicate that the animal has been ovariectomized. NOTE: This test has not been validated for, but has been used in, cats with similar interpretations as in dogs.

212 Microfilaria

Methodology: Modified Knotts

Specimen Requirements 1.0 mL Whole Blood, LTT

Reported Time Same Day

Collection Notes None

Additional Comments: Recommend testing only in dogs 7 months of age and older. Microfilaria are rarely detected in cats.Test included in Heartworm Panels, see Heartworm panel 58 or Feline Heartworm Panel 1629.

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328 Mycoplasma haemofelis (Hemobartonella)

Methodology: Microscopic Examination

Specimen Requirements 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Collection Notes None

Additional Comments: Exam of blood smear for Mycoplasma hemofelis (H. felis) is included in the CBC. Hemobartonella felis has been reclassified as Mycoplasma sp. Molecular testing (PCR) can differentiate Mycoplasma hemofelis (large form Hemobartonella) from Mycoplasma hemominutum (small form Hemobartonella). See test SO1638 Mycoplasma (Hemobartonella) hemofelis (PCR) for details.

8502 Oncology Profile

CBCGlucoseBUN (Blood Urea Nitrogen)

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same day

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)AlbuminCalcium

CreatinineTotal ProteinGlobulin

Collection Notes Please allow sample to clot (up to 20 minutes) and then spin down sample. If using a RTT, remove serum and put into a fresh RTT/CTT. Avoid lipemia and hemolysis.

A grey top tube is recommended but not necessary if the SST submitted has complete separation after centrifugation, or the RTT is centrifuged and serum is transferred to a transport tube.

Additional Comments: This test is for monitoring effects of chemotherapy.

213 Ova & Parasite

Methodology: Centrifugation/Microscopic

Specimen Requirements 5 grams Fresh Feces, Fecal Tube or bag

Reported Time Next day

Collection Notes None

Additional Comments: Test includes a direct smear as well as a sugar flotation with two centrifugation sequences; microscopic examination for ova and parasites, including Giardia cysts. Giardia/Crypto IFA add-on #241 is recommended to confirm the presence of Giardia as noted.

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2213 Ova & Parasite Combo w/IFA

Ova & Parasite

Specimen Requirements 10 grams of Fresh Feces, Fecal Tube or bag. Sample can not contain clay litter.

Reported Time Next Day

Giardia/Crypto IFA

Collection Notes Do not collect sample contaminated with clay kitty litter. It causes interference with IFA testing. Wheat/corn litter OK.

Additional Comments: Ova and parasite test includes a direct smear as well as a sugar flotation with two centrifugation sequences; microscopic examination for ova and parasites, including Giardia cysts. IFA testing will detect the antigen of Giardia species and most Cryptosporidia. It will not detect some species of Cryptosporidium that are associated with birds and reptiles.

For avian/non-mammalian testing, see Avian/Non-Mammalian Giardia/Cryptosporidium 1530.

239 Panleukopenia, Fecal

Methodology: RIM

Specimen Requirements 2 grams of Fresh Feces, Fecal Tube or bag.

Reported Time Same day

Collection Notes None

Additional Comments: FPV may only be detectable 24-48 hours post infection. False positives may occur up to two weeks post vaccination.

850 Pre-Op Panel

CBCGlucoseBUN (Blood Urea Nitrogen)

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

CreatinineTotal ProteinGlobulin

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)Albumin

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: None

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1851 Pre-Op Panel, Follow-up

CBCGlucoseBUN (Blood Urea Nitrogen)

Specimen Requirements Chemistry: 0.5 mL Serum,SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

CreatinineTotal ProteinAlbumin

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)Globulin

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

31 Protein Electrophoresis

Agarose Gel Electrophoresis

Specimen Requirements 0.25 mL Serum, SST/RTT. Can be run on heparinized plasma for avians/nonmammalians.

Reported Time Monday and Thursday, Same Day

Collection Notes None

Additional Comments: Test can also be performed on body cavity effusions to assist in diagnosis of FIP.

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300 Relaxin, Canine or Feline

Methodology: RIM

Specimen Requirements 0.5 mL Serum, SST/RTT.Test can also be run on heparinized or EDTA Plasma (GrnTT/LTT).Markedly hemolyzed or lipemic samples should not be used.

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Day 20 of gestation is found to be the earliest day of pregnancy detection. Relaxin rises after implantation of a fertilized egg. Implantation occurs 26-31 days after the LH surge. Any negative result is considered indicative of non-pregnancy; however, two negative results one week apart may be required for confirmation of this status, especially when date of mating is unknown. A positive result is considered indicative of pregnancy. A weak positive result may be observed at the beginning of the pregnancy. It is recommended to repeat the test on a fresh sample one week later to confirm pregnancy.

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1723 Relocated Feline Routine Screen

CBCUrinalysisHeartworm AntibodyAlbuminGlobulinAlb/Glob Ratio

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urine

Reported Time Same Day

GlucoseBUN ( Blood Urea Nitrogen)CreatinineBUN/Crea RatioTotal proteinFeline Heartworm Screen (in 6 months)

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)SodiumPotassiumChloride

Collection Notes Please indicate method of urine collection.Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Use code 17236 and include original accession number when submitting the 6 month follow up Feline Heartworm Panel. Follow Up testing must be submitted within 12 months.

5023 Renal Panel, Chronic

Methodology: Spectrophotometry

BUN (Blood Urea Nitrogen)Creatinine

Specimen Requirements 0.3 mL Serum, SST/RTT. Please call our Client Services for information about minimums on short samples.

Reported Time Same Day

Phosphorus Potassium

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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5025 Renal Profile

HCT (Hematocrit)Creatinine

Specimen Requirements 0.5 mL of each, Serum, SST/RTT0.1 mL Whole Blood, LTT.A sealed crit w/ blood also accepted in lieu of LTT.

Reported Time Same Day

PotassiumCalcium

PhosphorusBUN (Blood Urea Nitrogen)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Run every 3 to 6 months depending on clinical signs.

5027 Renal Profile w/ Urinalysis

UrinalysisHCT (Hematocrit)BUN (Blood Urea Nitrogen)

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTTUrinalysis: 5 mL fresh urine, Urine Tube/CTT

Reported Time Same Day

CreatininePhosphorus

PotassiumCalcium

Collection Notes Please indicate method of urine collection.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Run every 3 to 6 months depending on clinic signs.

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1716 Senior Routine Screen

CBCGlucoseBUN (Blood Urea Nitrogen)CreatinineBUN/Creat RatioSodiumPotassium

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urine

Reported Time Same Day

Sodium/Potassium RatioChlorideCalciumPhosphorusTotal ProteinAlbuminGlobulin

Albumin/Globulin ratioBilirubin TotalALP (Alkaline Phosphatase)ALT (Alanine Transaminase)Total T4Urinalysis

Collection Notes Please indicate method of urine collection. Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: None

11 Senior Screen

Methodology: Spectrophotometry

GlucosePotassiumPhosphorusChloride

Specimen Requirements 0.5 mL Serum SST/RTT

Reported Time Same Day

BUN (Blood Urea Nitrogen)CreatinineNa/K RatioTotal T4

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)Sodium

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

For a Senior Screen w/ CBC, order 899

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899 Senior Screen w/ CBC

CBCGlucosePotassiumSodium

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

PhosphorusBUN (Blood Urea Nitrogen)CreatinineChloride

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)Na/K RatioTotal T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

1101 Senior Screen w/Cholesterol

Methodology: Spectrophotometry

GlucosePotassiumSodiumNa/K Ratio

Specimen Requirements 0.5 mL Serum SST/RTT, 1.0 mL GTT

Reported Time Same Day

BUN (Blood Urea Nitrogen)CreatinineTotal T4Chloride

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)CholesterolPhosphorus

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

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651 T3 (Triiodothyronine)

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Mondays, Same day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Test is run every Monday.

9 T3 Suppression I

Methodology: Spectrophotometry

Pre Total T4

Specimen Requirements 2 SST/RTT, Pre T4/Post T4, 0.3 mL Serum each

Reported Time Same Day

Post Total T4

Collection Notes Protocol: 1.  Day 1:  Collect baseline (pre) T4 sample.  Label tube "Pre" 2.  Day 1 & 2:  Administer 25 ug PO of T3 supplement (Cytobin or Cytomel) every 8 hours for 7 doses. 3.  Day 3:  Administer the last (7th) dose of T3 supplement on the morning of the third day and 2-4 hours later draw sample for a second T4 determination. Label tube "Post".Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: T3 suppression is done to CONFIRM feline hyperthyroidism. Interpretation of Post-suppression T4 concentration: Normal cats:  < 1.5 ug/dL or suppression greater than 50% of pre-T4 value.Inconclusive:  1.5 - 2.0 ug/dL Hyperthyroid:  > 2.0 ug/dL. If the post T4 sample is not adequately suppressed, it may be due to: 1) the cat did not receive an adequate amount of T3, or 2) the cat is hyperthyroid. Measurement of T3 concentration in the pre and post suppression samples (Test 1009 - T3 Suppression II) can be useful in verifying that the patient received the hormone supplementation.

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1009 T3 Suppression II

Methodology: Chemiluminescent Immunoassay /Spectro

Pre and Post T4

Specimen Requirements Pre and Post required, 0.5 mL (minimum 0.3mL) serum each.

Reported Time Mondays, Same Day

Pre and Post T3

Collection Notes Protocol: 1. Day 1 - collect baseline (pre) T3 and T4. Label tube "Pre" 2. Day 1&2 - administer 25ug PO of T3 supplement Cytobin or Cytomel) every 8 hours for 7 doses.3. Day 3 - administer the last (7th) dose of T3 supplement on the morning of the third day and 2-4 hours later collect sample for a second T3 and T4 determination. Label tube "Post".Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: T3 suppression is done to CONFIRM feline hyperthyroidism. Submit both samples at one time to allow analysis on the same instrument run. Interpretation of Post-suppression T4 concentration: Normal cats:  < 1.5 ug/dL or suppression greater than 50% of pre-T4 value.Inconclusive:  1.5 - 2.0 ug/dL Hyperthyroid:  > 2.0 ug/dL. If the post T4 sample is not adequately suppressed, it may be due to: 1. The cat did not receive an adequate amount of T3 2. the cat is hyperthyroid.

75 Thyroid II

Total T4

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

TSH (Thyroid Stimulating Hormone)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: TSH only validated on canines, but has been used in felines. Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

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49 Thyroid Screen

Total T4

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time See Comment

T3 (Triiodothyronine)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: T4 run same day, T3 run every Monday. Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

689F TLI/Cobalamin/Folate (FELINE ONLY)

Methodology: Chemiluminescent Immunoassay/RIA

Specimen Requirements 1.5 mL Serum, SST/RTT. Sample MUST be non-hemolyzed for folate testing and avoid lipemic samples.

Reported Time See Comment

Collection Notes Fast animals 12-18 hours (diabetic animals 6-8 hours) before draw.Cover samples with foil, vet wrap, or a paper towel to protected from light (folate and cobalamin can be light sensitive).We strongly encourage that all serum samples be allowed to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Cobalamin and Folate are run in-house Monday, Wednesday, and Friday; reported out same day. Folates >24.0 ug/dL and cobalamin >1000 are re-run on dilution and reported. TLI are sent to Texas GI Lab, reported in 3-7 days.

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3 Total Body Diagnostic

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urine

Reported Time Same Day

AmylaseLipase

CK (Creatine Kinase)Urinalysis

Collection Notes Please indicate method of urine collection. Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: None

35 Total Body Diagnostic w/o Urinalysis

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

LipaseAmylase

CK (Creatine Kinase)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: If you would like this panel and a Urinalysis, See Total Body Diagnostic 3

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1035 Total Body Diagnostic w/o Urinalysis, Follow Up

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

AmylaseLipase

CK (Creatine Kinase)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

1003 Total Body Diagnostic, Follow-Up

CBCChemistry Screen

Specimen Requirements Chemistry: SST/RTT Minimum: 0.5 mL serum CBC: 2 air dried slides, 1.0mL LTT Urinalysis: 5mL urine

Reported Time Same Day

AmylaseLipase

CK (Creatine Kinase)Urinalysis

Collection Notes Please indicate method of urine collection.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

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159 Urinalysis

Specimen Requirements 5 mL Urine, Urine Tube/CTT

Reported Time Same Day

Collection Notes Please indicate method of collection.Submit sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Includes: Urine specific gravity run on analyzer, verified by refractometer, Chemistries (pH, Glucose, Protein, Ketones, Blood, and Bilirubin) run on Urine analyzer, and microscopic examination of sediment. Low volume samples will use dipstick readings. Low volumes may also be changed to Urine Sediment Only 155.

548 Urinalysis w/ C&S

Urinalysis

Specimen Requirements 5 mL Urine, Urine Tube/CTT. For the most accurate results 1 mL urine in sterile container should be submitted in addition to 5 mL urine.

Reported Time See Comment

Urine Culture

Collection Notes Please indicate method of urine collection.Do not submit syringes with needles, they are not accepted. Whenever possible, urine should be collected by cystocentesis. When this is not possible, free-catch urine into sterile container.Submit sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Turn around time is same day for the UA and 36 - 72 hours for Culture. Culture includes sensitivity panels for 2 pathogens. Additional sensitivities can be requested for an additional charge. No growth cultures are finalized after 48 hours of incubation. Urine collected on a swab or thio will not allow a colony count to be provided.

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552 Urinalysis w/ Culture, Follow-up

Urinalysis

Specimen Requirements 5 mL Urine, Urine Tube/CTT. For the most accurate results 1 mL urine in sterile container should be submitted in addition to 5 mL urine.

Reported Time See Comments

Urine Culture

Collection Notes Please indicate method of urine collection.Do not submit syringes with needles, they are not accepted. Whenever possible, urine should be collected by cystocentesis. When this is not possible, free-catch urine into sterile container.Submit sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Please include all antibiotic history with urine culture.Turn around time is same day for the UA and 36 - 72 hours for Culture. Culture includes sensitivity panels for 2 pathogens. Additional sensitivities can be requested for an additional charge. No growth cultures are finalized after 48 hours of incubation. Urine collected on a swab or thio will not allow a colony count to be provided.

Submit within 30 days of original test. Previous Case ID is required to activate the discounted price

234 Urine Cortisol/Creatinine Ratio

Urine Cortisol

Specimen Requirements 0.5 mL Urine, Urine Tube/CTTIf sample is bloody or very cloudy/cellular, submit a larger volume (1.0 mL minimum) as the sample will need to be centrifuged.

Reported Time Same Day

Urine Creatinine Ucor/Ucreat Ratio

Collection Notes If the patient has been on steroids for >14 days, discontinue for 2 weeks prior to testing. If <14 days, discontinue for 48 hours prior to testing.

Additional Comments: INTERPRETATION:An elevated urine cortisol/creatinine ratio (> 20) provides additional support for canine hyperadrenocorticism but is not specific for that condition. Many disorders cause an increased result. A normal result (<20) makes hyperadrenocorticism very unlikely. Further testing (low dose Dexamethasone Suppression Test and/or ACTH Stimulation Test) is advised to establish a diagnosis of hyperadrenocorticism.

*Note that both cortisol and creatinine have been converted to moles to get this ratio.

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551 Urine Culture, Follow-up

Methodology: Culture

Specimen Requirements 1.0 mL Urine, CTTCollected via cystocentesis preferred

Reported Time 36-72 hours

Collection Notes Whenever possible, urine should be collected by cystocentesis. If not possible, collect catheterized or free catch urine sample into sterile container. Refrigerate urine sample after collection.

Additional Comments: Please include all antibiotic history with urine culture.Colony counts are not possible if a plate, broth, or swab is submitted. Culture includes sensitivities on up to 2 isolates. Cultures are quantitated and reported in CFU/mL. No growth cultures are finalized after 48 hours of incubation.

Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.To include with a urinalysis, refer to Urinalysis with Culture, Follow-up 552.

1031 Urine Protein Electrophoresis

Methodology: Agarose Gel Electrophoresis

Specimen Requirements 5 mL Urine, CTT/Urine Tube

Reported Time Monday/Thursday

Collection Notes None

Additional Comments: Please submit one day before testing to allow for urine concentration.

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387 Vector/Tick Borne Disease Screen

Methodology: Lateral Flow

Specimen Requirements 0.5 mL Serum, SST/RTT. Plasma or whole blood (EDTA or heparinized) may be used.

Reported Time Daily AM - Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Test includes: Ehrlichia canis, Ehrlichia ewirgii, Ehrlichia chaffeensis, Anaplasma phagocytophilum, Anaplasma platys, Borrelia burgdorferi (Lyme Disease), and Heartworm (D. immitis).

Test methodology unable to differentiate between Anaplasma phagocytophilum and Anaplasma platys. Also unable to differentiate between Ehrlichia canis, Ehrlichia chaffeensis, and Ehrlichia ewingii.

Also see Canine Tick-borne Disease panel, SO666 if screening for Rocky Mountain Spotted Fever is desired.

1711 Wellness Screen II

CBCChemistry ScreenUrinalysis

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urine

Reported Time Same Day

AmylaseLipase

CK (Creatine Kinase)Total T4

Collection Notes Please indicate method of urine collection. Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: To request this panel, but submit the urinalysis portion later, see Wellness Screen II w/ Urine to Follow 1711NU

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1721 Wellness Screen II w/ SDMA

CBCChemistry Screen Urinalysis

Specimen Requirements Chemistry: 1.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urine

Reported Time See comment

AmylaseLipaseSDMA

CK (Creatine Kinase)Total T4

Collection Notes Please indicate method of collection.Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: CBC, Chemistries, and urinalysis run same day, SDMA Results Next Day. See SDMA SO513 for more details about test.

1712 Wellness Screen III

CBCChemistry ScreenFree T4

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same day

AmylaseLipase

CK (Creatine Kinase)Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Panel includes Wellness Screen I and Free T4. Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

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1713 Wellness Screen IV

CBCChemistry ScreenUrinalysis

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urine

Reported Time Same day

AmylaseLipaseFree T4

CK (Creatine Kinase)Total T4

Collection Notes Please indicate method of urine collection. Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

15 Wellness/Geriatric Panel

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

AmylaseLipase

CK (Creatine Kinase)Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: None

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1722 Wellness/Geriatric Panel w/ SDMA

CBCChemistry ScreenSDMA

Specimen Requirements Chemistry: 1.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time See comment

AmylaseLipase

CK (Creatine Kinase)Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: CBC and Chemistries run same day, SDMA results next day. See SDMA SO513 for more details about test.

1160 Wellness/Geriatric Panel w/Triglycerides

CBCChemistry ScreenTriglycerides

Specimen Requirements Chemistry: 0.5 mL serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT, air dried peripheral blood smears

Reported Time Same Day

AmylaseLipase

CK (Creatine Kinase)Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: None

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1015 Wellness/Geriatric Panel, Follow-Up

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT, air dried peripheral blood smears

Reported Time Same Day

AmylaseLipase

CK (Creatine Kinase)Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

881 Wellness/Geriatric Profile w/ Feline Viral I

CBCChemistry ScreenAmylase

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time See Comment

LipaseCK (Creatine Kinase)Total T4

FeLV (ELISA)FIV (IFA)

Test Alias: Feline Panel 881

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistry, CBC, and FeLV run same day, FIV run daily, AM. Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted. Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

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858 Wellness/Geriatric Profile w/ Feline Viral II

CBCChemistry ScreenAmylase

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time See Comments

LipaseCK (Creatine Kinase)Total T4

FeLV (ELISA)FIV (IFA)FCoV (FIP) Screen (IFA)

Test Alias: Total Feline Panel

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Chemistry, CBC, and FeLV run same day, FIV/FIP run daily, AM. Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted. Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

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Equine/Large Animal Panels

697 ACTH Plasma, Endogenous

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL EDTA Plasma, Separated and Frozen, CTT

Reported Time Mon/Wed/Fri, Same day

Collection Notes Spin down LTT immediately and separate from cells. Freeze as soon as possible. Frozen plasma stable for 14 days. Avoid grossly hemolyzed and/or lipemic samples

Additional Comments: Dexamethasone suppression test is no longer recommended as the primary test for diagnosis of PPID/Cushing's Disease in horses.  Endogenous ACTH is the preferred test.  Also see test code 6971 for information about TRH stimulation testing.

887 Bovine Chemistry Panel

Methodology: Spectrophotometry

AlbuminTotal BilirubinBUN (Blood Urea Nitrogen)GlucoseCreatinineCalcium

Specimen Requirements 0.5 mL Serum, SST/RTT, 1.0 mL GTT

Reported Time Same Day

MagnesiumPhosphorusPotassiumSodiumTotal ProteinAlb/Glob Ratio

ChlorideCholesterolCK (Creatine Kinase)GGT (Gamma-glutamyltransferase)Globulins

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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8871 Bovine Panel for Ca Supplement

Methodology: Spectrophotometry

AlbuminCalciumChlorideAlb/Glob Ratio

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

MagnesiumPhosphorusPotassium

SodiumTotal ProteinGlobulin

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

34 CBC w/ Fibrinogen and Plasma Protein

Methodology: Flow Cytometry/Refractometry

Specimen Requirements 1.5 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: None

24 CBC w/ Plasma Protein

Methodology: Flow Cytometry/Refractometry

Specimen Requirements 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: None

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98 Chemistry Screen, Large Animal

Methodology: Spectrophotometry

GlucoseBUN (Blood Urea Nitrogen)CreatinineSodiumPotassiumSodium/Potassium RatioChlorideCO2 (Carbon Dioxide)Anion Gap

Specimen Requirements 0.5 mL Serum, SST/RTT, 1.0 mL GTT

Reported Time Same Day

Calcium PhosphorusCa/Phos RatioCalculated OsmolalityTotal ProteinAlbuminGlobulinAlbumin/GlobulinBilirubin, Total

ALP (Alkaline Phosphatase)AST (Aspartate Transaminase)GGT (Gamma-glutamyltransferase)CholesterolCK (Creatine Kinase)AmylaseLipaseBile Acids

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

614 Coggins EIA, AGID

Methodology: AGID

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Up to 36 hours

Collection Notes The animal's name MUST be on the tube and MUST match the name listed on the form. Veterinarian signature and animal location is also REQUIRED.

Please allow sample to clot (up to 20 minutes) and then spin down sample. If using a RTT, remove serum and put into a fresh RTT/CTT. Avoid lipemia and hemolysis.

Additional Comments: USDA Equine Anemia Special Form or Global Vet Link Form required. Please print clearly as illegible documents can cause delay. Veterinarian signature and animal location are REQUIRED. Blood tube MUST be labeled with the animal's name that is listed on the accompanying form. If any of these are missing it can cause a delay or return of sample and/or form for corrections (additional charges may apply).

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601 Coggins, ELISA

Methodology: ELISA

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes The animal's name MUST be on the tube and MUST match the name listed on the form. Veterinarian signature and animal location is also REQUIRED.

Please allow sample to clot (up to 20 minutes) and then spin down sample. If using a RTT, remove serum and put into a fresh RTT/CTT. Avoid lipemia and hemolysis.

Additional Comments: USDA Equine Anemia Special Form or Global Vet Link Form required. Please print clearly, as illegible documents can cause delay. Veterinarian signature and animal location are REQUIRED. Blood tube MUST be labeled with the animal's name that is listed on the accompanying form. If any of these are missing it can cause a delay or return of sample and/or form for corrections (additional charges may apply).

508 Culture, Equine Uterine

Methodology: Culture

Specimen Requirements Bacterial culture swab with transport medium

Reported Time 2-5 Days

Collection Notes None

Additional Comments: Normal test value is "No Beta-hemolytic streptococci isolated."

Refer to test #862 Equine Uterine Screen for combo test with uterine cytology.

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2038 Dexamethasone Suppression, Equine

Methodology: Chemiluminescent Immunoassay

Cortisol Pre

Specimen Requirements Pre and Post required, 0.5 mL (minimum 0.3mL) serum each, RTT ONLY

Reported Time Same Day

Cortisol 19-hr Post

Collection Notes PROTOCOL: DO NOT USE SST TO COLLECT OR SPINDOWN SAMPLE1.  Begin test between 4-6 PM. 2.  Draw a pre-injection blood sample into a RTT and label PRE. 3.  Inject 40 ug/kg of DEXAMETHASONE intramuscularly. 4.  Nineteen hours post-injection, draw a blood sample into a RTT and label POST-19.  5. Blood samples should be centrifuged, the serum separated, placed in a clean tube, and refrigerated or frozen until submitted to the Lab. 6.  Submit both samples at one time.

Additional Comments: This test is no longer recommended as the primary test for diagnosis of Pituitary Pars Intermedia Dysfunction (PPID)/Equine Cushing's Disease.  Please see test code 697 (ACTH, plasma) for additional information on testing for PPID. Normal horses: Cortisol </= 1.0 ug/dL 19 hours post dexamethasone. Interpret with clinical signs and rule out other conditions. Also, if performing this test in the fall, starting in September, interpret a failure to suppress with caution as false positives occasionally occur at that time of the year. Prednisone, prednisolone, and other corticosteroids (except dexamethasone) will cross react. Recommend these drugs be discontinued at least 24 hrs before testing.

30 Equine Chemistry Screen

Methodology: Spectrophotometry

GlucoseBUN (Blood Urea Nitrogen)CreatinineSodiumPotassiumSodium/Potassium RatioChlorideCarbon Dioxide (CO2)

Specimen Requirements 1.0 mL Serum, SST/RTT, 1.0 mL GTT

Reported Time Same Day

Anion GapCalciumPhosphorusCalculated OsmolalityTotal ProteinAlbuminGlobulinAlbumin/Globulin ratio

Bilirubin TotalALP (Alkaline Phosphatase)AST (Aspartate Transaminase)GGT (Gamma-glutamyltransferase)CholesterolCK (Creatine Kinase)Sorbitol Dehydrogenase (SDH)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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8 Equine General Endocrine

Equine Chemistry Screen

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTTACTH Endo: 0.5 mL EDTA Plasma, Separated and Frozen, CTT.

Reported Time See Comments

ACTH Endogenous Total T4

Collection Notes For ACTH: Spin down LTT immediately and separate from cells. Freeze as soon as possible.Frozen plasma stable for 14 days. Avoid Grossly hemolyzed and/or lipemic samplesFor Chemistry: Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Dexamethasone suppression test is no longer recommended as the primary test for diagnosis of PPID/Cushing's Disease in horses.  Endogenous ACTH is the preferred test.  Chemistry Screen and Total T4 done same day.ACTH Endogenous run Mon/Wed/Fri with results out same day.Also see test code 6971 for information about TRH stimulation testing.

12 Equine Immunodeficiency

CBC

Specimen Requirements IgG/IgM minimum: 0.2 mL Serum CBC: 1.0 mL LTT (0.5 mL minimum), Air dried peripheral blood smears

Reported Time See comment

Immunoglobulin M, Equine Immunoglobulin G, Equine

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Results for CBC will be out same day, IgG/IgM results out next day (24 hr final) A 4hr preliminary can be requested by calling laboratory.

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1634 Equine Insulin/ACTH, Endogenous

Insulin

Specimen Requirements Insulin: 0.5 mL serum, Frozen, RTT/CTTEndogenous ACTH: 1.0 mL EDTA Plasma, separated and frozen, CTT.

Reported Time Mon/Wed/Fri, Same day

Glucose Endogenous ACTH

Collection Notes For ACTH, Endo: Spin down LTT immediately and separate from cells. Freeze as soon as possible. Frozen plasma stable for 14 days. Avoid Grossly hemolyzed and/or lipemic samples.For Insulin: Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection; this limits hemolysis which can affect results. Separate serum into transport tube and freeze.Avoid lipemia and hemolysis.

Additional Comments: Report includes reference ranges and interpretation. Equine insulin and glucose levels have been suggested for diagnosing insulin dysregulation, but can be abnormal in any disease causing insulin resistance. For more information, please visit our website to view our test service update on Equine insulin testing.Testing not recommended on samples >24 hours old due to rapid deterioration.

750 Equine Pre-Op Panel

CBCFibrinogenGlucose

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT 1.0 mL GTT CBC: 1.5 mL LTT and air dried peripheral blood smears

Reported Time Same Day

BUN (Blood Urea Nitrogen)Total ProteinAlbumin

GlobulinGGT (Gamma-glutamyltransferase)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Fibrinogen performed by heat precipitation..

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4 Equine Screen

CBCEquine Chemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.5 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Fibrinogen Plasma Protein

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: SDH is included in Equine Chemistry Screen.Fibrinogen performed by heat precipitation.

89 Equine Screen, Follow-Up

CBCEquine Chemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.5 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Fibrinogen Plasma Protein

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: SDH is included in Equine Chemistry Screen. Fibrinogen performed by heat precipitation.

Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

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2404 Equine Senior Wellness I

CBCFibrinogenPlasma Protein

Specimen Requirements Chemistry/Insulin: 1.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.5 mL LTT and air dried peripheral blood smearsACTH Endo: 1.0 mL Frozen EDTA Plasma, CTT.Samples for Insulin testing CAN NOT be hemolyzed.

Reported Time See comment

InsulinGlucoseACTH Endogenous

Total T4Plasma Glucose

Collection Notes The LTT for ACTH Endo MUST be spun down within 30 minutes of collection and the plasma separated into a non-additive tube such as a CTT. Freeze until transported to lab. Insulin samples MUST be spun down within 30 minutes; if submission is to be delayed, draw a second tube or, volume allowing, pull some serum off the SST/RTT and Freeze for insulin testing.Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 30 minutes of collection; this then does not require collection of a GTT; this also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Equine insulin and glucose levels have been suggested for diagnosing insulin dysregulation, but can be abnormal in any disease causing insulin resistance. For more information, please visit our website to view our test service update on Equine insulin testing.Testing not recommended on samples >24 hours old due to rapid deterioration.

Chemistries and CBC reported out same day. ACTH Endo and Insulin run Monday/Wednesday/Friday, reported out same day.

Fibrinogen performed by heat precipitation.

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2405 Equine Senior Wellness II

CBCFibrinogen

Specimen Requirements Chemistry/Insulin: 2.0 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.5 mL LTT and air dried peripheral blood smearsACTH Endo: 1.0 mL Frozen EDTA Plasma, CTT. Samples for Insulin testing CAN NOT be hemolyzed.

Reported Time See comment

InsulinACTH Endogenous

Total T4Equine Chem Screen

Collection Notes The LTT for ACTH Endo MUST be spun down within 30 minutes of collection and the plasma separated into a non-additive tube such as a CTT. Freeze until transported to lab. Insulin samples MUST be spun down within 30 minutes; if submission is to be delayed, draw a second tube or, volume allowing, pull some serum off the SST/RTT and freeze for insulin testing. Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 30 minutes of collection; this then does not require collection of a GTT; this also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: SDH is included in Equine Chemistry Screen.Equine insulin and glucose levels have been suggested for diagnosing insulin dysregulation, but can be abnormal in any disease causing insulin resistance. For more information, please visit our website to view our test service update on Equine insulin testing.Testing not recommended on samples >24 hours old due to rapid deterioration.

Chemistries and CBC reported out day. ACTH, Endo and Insulin run Monday/Wednesday/Friday, reported out same day.

Fibrinogen performed by heat precipitation.

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2872 Equine Special w/ Insulin

CBCFibrinogen

Specimen Requirements Chemistry/Insulin: 1.0 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.5 mL LTT and air dried peripheral blood smears.

Reported Time See Comment

Total T4Equine Chem Screen

Insulin

Collection Notes Insulin samples MUST be spun down within 30 minutes; if submission is to be delayed, draw a second tube or, volume allowing, pull some serum off the SST/RTT and freeze for insulin testing. Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 30 minutes of collection; this then does not require collection of a GTT; this also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: SDH is included in Equine Chemistry Screen.Equine insulin and glucose levels have been suggested for diagnosing insulin dysregulation, but can be abnormal in any disease causing insulin resistance. For more information, please visit our website to view our test service update on Equine insulin testing.Testing not recommended on samples >24 hours old due to rapid deterioration.

Chemistry and CBC results out same day.Insulin run Monday/Wednesday/Friday with results out same day.

Fibrinogen performed by heat precipitation.

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40 Equine Total Body

CBCEquine Chemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.5 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urine

Reported Time Same Day

Fibrinogen Urinalysis

Collection Notes Please indicate method of urine collection. Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Submit sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: SDH is included in Equine Chemistry Screen.

Fibrinogen performed by heat precipitation.

1409 Fecal Parasite, Quantitative

Methodology: Centrifugation/Microscopic

Specimen Requirements 1.0 gram Fresh Feces, Fecal tube or bag

Reported Time Next Day

Collection Notes None

Additional Comments: Results will be reported out as type of parasite and number of eggs per gram of feces.

325 Fibrinogen, Semi-quantitative

Methodology: Heat Precipitation

Specimen Requirements 0.5 mL Plasma (1.0 mL Whole Blood) LTT or BTT

Reported Time Same Day

Collection Notes None

Additional Comments: If a BTT is used, it will be corrected for the citrate dilution factor.

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852 IgG, Camelid RID

Methodology: Radial Immunodiffusion (RID)

Specimen Requirements 0.3 mL Serum, SST/RTT

Reported Time 24 Hours

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Acceptable for Alpaca, Llama, and Camel. A 4-hr preliminary result is available upon request.

210 IgG, Equine RID

Methodology: Radial Immunodiffusion (RID)

Specimen Requirements 0.25 mL Serum, SST/RTT. Colostrum can also be used.

Reported Time 24 Hours

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: A 4-hr preliminary result is available upon request. Final result at 24 hours.

211 IgM, Equine RID

Methodology: Radial Immunodiffusion (RID)

Specimen Requirements 0.25 mL Serum, SST/RTT. Colostrum can also be used.

Reported Time 24 Hours

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Final result at 24 hours.

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634 Insulin

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 1.0 mL Serum, Minimum: 0.5 mL, SST/RTT, Frozen**Serum can NOT be hemolyzed as hemolysis will interfere with testing.**

Reported Time Mon, Wed, Fri, Same day

Collection Notes Centrifuge tube within 20-30 minutes of collection. Separate serum into a transport tube. Sample should be tested or frozen within 24 hours. If unable to centrifuge, use GTT for collection of glucose samples. GTT tubes must have a minimum of 1 mL of blood for accurate results.

Additional Comments: In combination with clinical signs and concurrent blood glucose concentration, determination of serum insulin may be useful in diagnosing an insulinoma. In assessing whether the serum insulin concentration is appropriate, it must be compared to the serum glucose concentration (taken concurrently; included in the cost of insulin test). Measurement of serum insulin concentrations is most useful when the blood glucose concentration is 59 mg/dL or less.Equine insulin and glucose levels have been suggested for diagnosing pituitary adenoma/Cushing's disease, but can be abnormal in any disease causing insulin resistance. Testing not recommended on samples >24 hours old due to rapid deterioration.

12085 Large Animal Liver Chemistry

Methodology: Spectrophotometry

AlbuminALP (Alkaline Phosphatase)Total Bilirubin

Specimen Requirements 0.5 mL Serum SST/RTT

Reported Time Same Day

Sorbitol Dehydrogenase (SDH)Total ProteinGlobulin

Direct BilirubinGGT (Gamma-glutamyltransferase)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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341 Occult Blood

Methodology: Oxidation

Specimen Requirements 0.5 grams of Fresh Feces, Fecal Tube or bag.Fecal sample should be at least pea sized.See Collection Notes on dietary restrictions.

Reported Time Same Day

Collection Notes Patient should be on a high residue diet starting 48 hours prior to sample collection. (This excludes pet foods containing Red or rare meat or poultry products, raw fruits and vegetables containing high peroxidase activity. Please call our client service department with any questions.) High doses of vitamin C (>250 mg/day) can cause false negative results. Discontinue supplements, fruits and vegetables high in vitamin C for 48 hours prior to testing.

Additional Comments: Consuming a meat diet could result in false positives. See Collection Notes. Anti-inflammatory medicines and other diseases of the gastrointestinal tract may cause false positiveGastrointestinal lesions may bleed intermittently and blood in feces is not distributed uniformly, thus a negative results does not rule out the possability of gastrointestinal lesions.

851 Pre-Op Panel, Large Animal

CBCGlucoseBUN (Blood Urea Nitrogen)

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

CreatinineTotal ProteinAlbumin

ALP (Alkaline Phosphatase)AST (Aspartate Transaminase)Globulin

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: None

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647 Progesterone, Non-Canine

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum, RTTMinimum: 0.3 mL Serum

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.Remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Mare: Corpus luteal values are 7-8 ng/mL by 6 days after ovulation. There is a transient decrease at 28 days of pregnancy to around 5 ng/mL and then concentration increases to day 50 and plateaus at day 140 (15-20 ng/mL). Values then decline so that at 180-200 days levels are below 1 ng/mL. Cow: Levels of 6 ng/mL are required to maintain pregnancy. Camelids: Values of about 2.0 ng/mL indicate luteal activity.

For canines, please order Canine Progesterone 547

6 Ruminant Screen

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.5 mL LTT and air dried peripheral blood smears

Reported Time Same Day

FibrinogenMagnesium

CK (Creatine Kinase)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Fibrinogen performed by heat precipitation

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1106 Ruminant Screen, Follow-Up

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL serum; CBC: 1.0 mL LTT, Air dried peripheral blood smears

Reported Time Same Day

MagnesiumFibrinogen

CK (Creatine Kinase)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Fibrinogen performed by heat precipitation

Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

131 Sorbitol Dehydrogenase (SDH)

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This test is included in the equine chemistry screen and all panels that include equine chemistries.

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872 Special Equine Profile

CBCEquine Chemistry Screen

Specimen Requirements Chemistry: 1.0 mL Serum,SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Total T4 Fibrinogen

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: SDH is included in Equine Chemistry Screen.

651 T3 (Triiodothyronine)

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Mondays, Same day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Test is run every Monday.

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6971 TRH Stimulation Test

Methodology: Chemiluminescent Immunoassay

ACTH, Endo Pre

Specimen Requirements 0.5 mL EDTA Plasma for Pre and Post samples, Separated and Frozen, CTT

Reported Time Mon/Wed/Fri, Same day

ACTH, Endo Post

Collection Notes Procedure:HORSES CAN BE FASTED OR MAY HAVE BEEN RECENTLY FED HAY, BUT NOT GRAIN. DO NOT PERFORM IMMEDIATELY AFTER AN ORAL SUGAR TEST.1. Collect 0 hour (PRE) TRH sample in LTT. Spin and separate plasma into a plastic transport tube within 1 hour of collection. Recommended to freeze immediately. Clearly label as 0 hour / PRE-sample.2.Inject 1 mg TRH (Tyrotropin Releasing Hormone) intravenously (IV) for horses >250 kg; inject 0.5 mg TRH IV for horses and ponies <250 kg. TRH can be obtained from compounding pharmacies.The online pharmacy Wedgewood sells TRH under the brand name Protirelin.3. At exactly 10 minutes after administration of TRH, collect second sample in LTT. Spin and separate plasma into a plastic transport tube within 1 hour of collection. Recommended to freeze immediately. Clearly label tube 10 minutes /POST-sample.

Additional Comments: Endogenous ACTH is tested on both samples. Recommended to perform test mid-November to mid-July since seasonally adjusted reference intervals have not yet been established. This is currently the recommended test for early detection of equine Pituitary Pars Intermedia Dysfunction (PPID) if a horse has clinical signs, and endogenous ACTH is inconclusive/negative.Transient yawning, coughing, mouth chomping and display flehmen response can follow administration of TRH. These side effects will subside within a few minutes.

159 Urinalysis

Specimen Requirements 5 mL Urine, Urine Tube/CTT

Reported Time Same Day

Collection Notes Please indicate method of collection.Submit sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Includes: Urine specific gravity run on analyzer, verified by refractometer, Chemistries (pH, Glucose, Protein, Ketones, Blood, and Bilirubin) run on Urine analyzer, and microscopic examination of sediment. Low volume samples will use dipstick readings. Low volumes may also be changed to Urine Sediment Only 155.

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862 Uterine Screen, Equine

Uterine Culture

Specimen Requirements Saline Flush in sterile container (CTT) is the preferred sample. (Allows for cytospin prep which provides the best cell interpretation and can be used for both cytology and culture). OR 2 transport swabs AND freshly made slides are OK

Reported Time See Comment

Uterine Cytology

Collection Notes Slides are ideally prepared immediately after collection. Prepare the cytology sample by GENTLY ROLLING (NOT SMEARING) the swab over the slide.This technique will deliver optimal cellular material for review.  Thoroughly dry the smears. DO NOT put slides into the slide holder until completely dry or all the cells will rupture. Do not refrigerate slides.

Please note: Slides prepared at lab from swabs have variable quality. Smears prepared on non-sterile slides may result in contamination of the culture.

Additional Comments: Cytology reported the same day (Monday - Saturday on Days). Culture report time 36 - 72 hours.

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Avian and Exotic Sample Guidelines Diagnostics for small avian and exotic patients presents very unique challenges. Small patient sizes and patient handling considerations can make it exceptionally difficult to obtain a sufficient amount for testing. When possible, please obtain sample volumes for testing as outlined below.

For best CBC results, always submit slides made directly from fresh blood (no EDTA or heparin exposure). This preserves the morphology of the cells and allows for the most accurate evaluation.

Panel Serum/plasma required

Approx. whole blood required (for avians/reptiles/non-ferret sm mammals with a normal Hct)

Approx. whole blood required (for ferrets with a normal Hct)

Electrolytes only 100 µL (0.1 mL) 0.25 mL 0.4 mL Single chemistry test 100 µL (0.1 mL) 0.25 mL 0.4 mL 10 chemistry Screen 100 µL (0.1 mL) 0.25 mL 0.4 mL 13 chemistry Screen 150 µL (0.15 mL) 0.35 mL 0.6 mL 18 chemistry Screen 200 µL (0.2 mL) 0.45 mL 0.7 mL 21 chemistry Screen 250 µL (0.2 mL) 0.55 mL 0.9 mL

If it is not possible to obtain these sample volumes, consider selecting a Mini Panel that prioritizes analytes and allows the veterinarian to direct diagnostics based on clinical impression. Phoenix Lab Mini Panels typically require 100-150 uL (0.1-0.15 mL) of plasma or serum and were created with the support of a boarded avian and exotic specialist to target the most important analytes for common conditions.

Whenever possible, please prioritize analytes or submit a back-up panel request in case of insufficient sample volume. This allows the lab to perform and result testing without delay.

Good sample collection relies heavily on using the appropriate tubes for collection. Below are the commonly used abbreviations for avian and exotic sample collection.

Abbreviation Corresponding tube RTT Red top tube M-RTT Micro red top tube M-SST Micro serum separator m-GnTT Micro green top tube m-LTT Micro lavender top tube

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Avian/Non-Mammalian Panels

3003 Avian Liver Chemistry

Methodology: Spectrophotometry

AlbuminAST (Aspartate Transaminase)

Specimen Requirements 0.3 mL heparinized plasma (minimum 100 µL) or 250 µL whole blood, m-GrnTTPlease call our Client Services for information about minimums on short samples.

Reported Time Same Day

CholesterolCK (Creatine Kinase)

Bile Acids

Collection Notes None

Additional Comments: None

1187 Avian Mini Liver Panel

Methodology: Spectrophotometry

Total ProteinAlbumin

Specimen Requirements 0.2 mL Serum, m-SST/m-RTT (Minimum: 0.1 mL)Heparinized plasma or 0.25 mL whole blood from m-GrnTT is also accepted.

Reported Time Same Day

CK (Creatine Kinase)Bile Acids

AST (Aspartate Transaminase)GGT (Gamma-glutamyltransferase)

Collection Notes None

Additional Comments: None

5024 Avian Mini Renal Panel

Methodology: Spectrophotometry

Uric AcidPhosphorous

Specimen Requirements 0.2 mL Serum m-SST/m-RTT (minimum: 0.1 mL Serum). Heparinized plasma or 250 µL whole blood from m-GrnTT is also accepted.

Reported Time Same day

BUN (Blood Urea Nitrogen) CalciumCa/Phos Ratio

Collection Notes None

Additional Comments: None

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7 Avian Panel

WBC Est/DiffHematocrit (HCT)GlucoseCalcium

Specimen Requirements Chemistry: 0.3 mL (minimum: 100 µL), heparinized plasma or 250 µL Whole Blood, m-GrnTTCBC: 2 air dried slides and 0.1 mL (Absolute minimum: 30 µL) of either heparinized or EDTA whole blood. Heparin preferred for some species (e.g. Crows, Jays)Please call our Client Services for information about minimums on short samples.

Reported Time Same Day

Total ProteinAST (Aspartate Transaminase)CK (Creatine Kinase)Cholesterol

Uric AcidBile AcidPhosphorusCa/Phos Ratio

Collection Notes One m-GrnTT can be used, volume allowing. Submit m-GrnTT without centrifugation in order for HCT and WBC to be done on same tube. Prepare Slides at clinic.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: If a microtainer (M-GrnTTor M-LTT) is not submitted, WBC is estimated from the blood smear.

82 Avian Panel, Follow-Up

WBC Est/DiffHematocrit (HCT)GlucoseCalcium

Specimen Requirements Chemistry: 0.3 mL (minimum: 100 µL), heparinized plasma or 250 µL Whole Blood, m-GrnTTCBC: 2 air dried slides and 0.1 mL (Absolute minimum: 30 µL) of either heparinized or EDTA whole blood. Heparin preferred for some species (e.g. Crows, Jays)Please call our Client Services for information about minimums on short samples.

Reported Time Same Day

Total ProteinAST (Aspartate Transaminase)CK (Creatine Kinase)Cholesterol

Uric AcidBile AcidPhosphorusCa/Phos Ratio

Collection Notes One m-GrnTT can be used, volume allowing. Submit m-GrnTT without centrifugation in order for HCT and WBC to be done on same tube. Prepare Slides at clinic.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: If a microtainer (M-GrnTT or M-LTT) is not submitted, WBC is estimated from the blood smear. Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

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3002 Avian Renal Chemistry

Methodology: Spectrophotometry

BUN (Blood Urea Nitrogen)CalciumPhosphorus

Specimen Requirements 0.3 mL heparinized Plasma (minimum 100 µL) or 250 µL Whole Blood, m-GrnTTPlease call our Client Services for information about minimums on short samples.

Reported Time Same Day

Ca/Phos RatioTotal ProteinAlbumin

GlobulinA/G RatioUric Acid

Collection Notes None

Additional Comments: None

3004 Avian/ Nonmammalian 13 Chemistry Screen

Methodology: Spectrophotometry

GlucoseCalciumPhosphorusCa/Phos RatioTotal Protein

Specimen Requirements 0.3 mL heparinized Plasma (Minimum: 150µL) or 350 µL Whole Blood, m-GrnTTPlease call our Client Services for information about minimums on short samples.

Reported Time Same Day

AlbuminGlobulinA/G RatioAST (Aspartate Transaminase)

CK (Creatine Kinase)CholesterolUric AcidBile Acids

Collection Notes None

Additional Comments: None

907 Avian/Nonmammalian 10-Chemistry Screen

Methodology: Spectrophotometry

GlucoseCalciumTotal ProteinCK (Creatine Kinase)

Specimen Requirements 0.3 mL (minimum: 100 µL) heparinized plasma or 250 µL Whole Blood, m-GrnTTPlease call our Client Services for information about minimums on short

Reported Time Same Day

CholesterolUric AcidBile Acids

AST (Aspartate Transaminase)PhosphorusCa/Phos Ratio

Collection Notes None

Additional Comments: None

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3005 Avian/Nonmammalian 13 Chemistry w/ CBC

CBCGlucoseCalciumPhosphorusCa/Phos Ratio

Specimen Requirements Chemistry: 0.3 mL (minimum 150 µL), heparinized plasma or 350 µL Whole Blood, m-GrnTTCBC: 2 air dried slides and 0.1 mL (Absolute minimum: 30 µL) of either heparinized or EDTA whole blood. Heparin preferred for some species (e.g. Crows, Jays)Please call our Client Services for information about minimums on short samples.

Reported Time Same Day

Total ProteinAlbuminGlobulinA/G RatioAST (Aspartate Transaminase)

CK (Creatine Kinase)CholesterolUric AcidBile Acids

Collection Notes One m-GrnTT can be used, volume allowing. Submit m-GrnTT without centrifugation in order for HCT and WBC to be done on same tube. Prepare Slides at clinic.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: If a microtainer (m-GrnTT or m-LTT) with whole blood is not submitted, WBC is estimated from the blood smear and PCV will be cancelled.

3006 Avian/Nonmammalian 13 Chemistry w/ CBC and PEP

CBCGlucoseCalciumPhosphorusCa/Phos Ratio

Specimen Requirements Chemistry: 0.3 mL (minimum 175 µL), heparinized plasma or 350 µL Whole Blood, m-GrnTTCBC: 2 air dried slides and 0.1 mL (Absolute minimum: 30 µL) of either heparinized or EDTA whole blood. Heparin preferred for some species (e.g. Crows, Jays)Please call our Client Services for information about minimums on short samples.

Reported Time See Comment

Total ProteinAlbuminGlobulinA/G RatioPEP (Protein Electrophoresis)

AST (Aspartate Transaminase)CK (Creatine Kinase)CholesterolUric AcidBile Acids

Collection Notes One m-GrnTT can be used, volume allowing. Submit m-GrnTT without centrifugation in order for HCT and WBC to be done on same tube. Prepare Slides at clinic.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistries and CBC reported out same day. PEP run Monday and Thursday with results out same day.If a microtainer (m-GrnTT or m-LTT) with whole blood is not submitted, WBC is estimated from the blood smear

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3010 Avian/Nonmammalian 13 Chemistry w/ CBC, Follow-up

CBCGlucoseCalciumPhosphorusCa/Phos Ratio

Specimen Requirements Chemistry: 0.3 mL (minimum 150 µL) heparinized plasma or 350 µL Whole Blood, m-GrnTTCBC: 2 air dried slides and 0.1 mL (Absolute minimum: 30 µL) of either heparinized or EDTA whole blood.Heparin preferred for some species (e.g. Crows, Jays)Please call our Client Services for information about minimums on short samples.

Reported Time Same Day

Total ProteinAlbuminGlobulinA/G RatioBile Acids

AST (Aspartate Transaminase)CK (Creatine Kinase)CholesterolUric Acid

Collection Notes One m-GrnTT can be used, volume allowing. Submit m-GrnTT without centrifugation in order for HCT and WBC to be done on same tube. Prepare Slides at clinic.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: If a microtainer (m-GrnTT or m-LTT) with whole blood is not submitted, WBC is estimated from the blood smear.

Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

3008 Avian/Nonmammalian 18 Chemistry Panel

Methodology: Spectrophotometry

GlucoseSodiumPotassiumSodium/Potassium RatioChloride, CalciumPhosphorus

Specimen Requirements 0.3 mL heparinized Plasma( Minimum: 0.2 mL) or 450 µL Whole Blood, m-GrnTTPlease call our Client Services for information about minimums on short samples.

Reported Time Same Day

AST (Aspartate Transaminase)CholesterolCK (Creatine Kinase)Uric Acid Bile Acids

Ca/Phos RatioTotal ProteinAlbuminGlobulinA/G RatioGGT (Gamma-glutamyltransferase)

Collection Notes None

Additional Comments: None

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3009 Avian/Nonmammalian 18 Chemistry w/ CBC

CBCGlucoseSodiumPotassiumSodium/Potassium RatioChloride, CalciumPhosphorus

Specimen Requirements Chemistry: 0.3 mL (minimum: 0.2 mL) heparinized plasma or 450 µL Whole Blood, m-GrnTTCBC: 2 air dried slides and 0.1 mL (Absolute minimum: 30 µL) of either heparinized or EDTA whole blood. Heparin preferred for some species (e.g. Crows, Jays)Please call our Client Services for information about minimums on short samples.

Reported Time Same Day

AST (Aspartate Transaminase)CholesterolCK (Creatine Kinase)Uric AcidBile Acids

Ca/Phos RatioTotal ProteinAlbuminGlobulinA/G RatioGGT (Gamma-glutamyltransferase)

Collection Notes One m-GrnTT can be used, volume allowing. Submit m-GrnTT without centrifugation in order for HCT and WBC to be done on same tube. Prepare Slides at clinic.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: If a microtainer (m-GrnTT or m-LTT) with whole blood is not submitted, WBC is estimated from the blood smear

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3012 Avian/Nonmammalian 18 Chemistry w/ CBC and Protein Electrophoresis

CBCPEP (Protein Electrophoresis)GlucoseSodiumPotassiumSodium/Potassium RatioChloride, CalciumPhosphorus

Specimen Requirements Chemistry: 0.3 mL (minimum: 0.25 mL) heparinized plasma or 550 µL Whole Blood, m-GrnTTCBC: 2 air dried slides and 0.1 mL (Absolute minimum: 30 µL) of either heparinized or EDTA whole blood.Heparin preferred for some species (e.g. Crows, Jays) Please call our Client Services for information about minimums on short samples.

Reported Time See comment

AST (Aspartate Transaminase)CholesterolCK (Creatine Kinase)Uric AcidBile AcidsPEP (Protein Electrophoresis)

Ca/Phos RatioTotal ProteinAlbuminGlobulinA/G RatioGGT (Gamma-glutamyltransferase)

Collection Notes One m-GrnTT can be used, volume allowing. Submit m-GrnTT without centrifugation in order for HCT and WBC to be done on same tube. Prepare Slides at clinic.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistries and CBC reported out same day. PEP run Monday and Thursday with results out same day. If a microtainer (m-GrnTT or m-LTT) with whole blood is not submitted, WBC is estimated from the blood smear

3013 Avian/Nonmammalian 21 Chemistry

Methodology: Spectrophotometry

GlucoseBUN (Blood Urea Nitrogen)SodiumPotassiumSodium/Potassium RatioChlorideCalcium

Specimen Requirements 0.3 mL heparinized plasma (Minimum: 0.25 mL) or 550 µL Whole Blood, m-GrnTT Please call our Client Services for information about minimums on short

Reported Time Same Day

AST (Aspartate Transaminase)CholesterolTriglyceridesCK (Creatine Kinase)Uric AcidAmylaseBile Acids

PhosphorusCa/Phos RatioTotal ProteinAlbuminGlobulinA/G RatioGGT (Gamma-glutamyltransferase)

Collection Notes None

Additional Comments: None

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3015 Avian/Nonmammalian 21-Chemistry w/ CBC

CBCGlucoseBUN (Blood Urea Nitrogen)SodiumPotassiumSodium/Potassium RatioChlorideCalcium

Specimen Requirements Chemistry: 0.3 mL heparinized plasma (minimum 250 µL) or 550 µL of whole blood.CBC: 2 air dried slides and 0.1 mL of either heparinized or EDTA whole blood. Heparin preferred for some species (e.g. Crows, Jays)Please call our Client Services for information about minimums on short samples.

Reported Time Same Day

AST (Aspartate Transaminase)CholesterolTriglyceridesCK (Creatine Kinase)Uric AcidAmylaseBile Acids

PhosphorusCa/Phos RatioTotal ProteinAlbuminGlobulinA/G RatioGGT (Gamma-glutamyltransferase)

Collection Notes One m-GrnTT can be used, volume allowing. Submit m-GrnTT without centrifugation in order for HCT and WBC to be done on same tube. Prepare Slides at clinic.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: If a microtainer (m-GrnTT or m-LTT) with whole blood is not submitted, WBC is estimated from the blood smear and PCV will be cancelled.

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3016 Avian/Nonmammalian 21-Chemistry w/ CBC and PEP

CBCGlucoseBUN (Blood Urea Nitrogen)SodiumPotassiumSodium/Potassium RatioChlorideCalcium

Specimen Requirements Chemistry: 0.3 mL (minimum: 0.27 mL) heparinized plasma or 550 µL Whole BloodCBC: 2 air dried slides and 0.1 mL (Absolute minimum: 30 µL) of either heparinized or EDTA whole blood.Heparin preferred for some species (e.g. Crows, Jays)Please call our Client Services for information about minimums on short samples.

Reported Time See comment

AST (Aspartate Transaminase)CholesterolTriglyceridesCK (Creatine Kinase)Uric AcidAmylaseBile AcidsPEP (Protein Electrophoresis)

PhosphorusCa/Phos RatioTotal ProteinAlbuminGlobulinA/G RatioGGT (Gamma-glutamyltransferase)

Collection Notes One m-GrnTT can be used, volume allowing. Submit m-GrnTT without centrifugation in order for HCT and WBC to be done on same tube. Prepare Slides at clinic.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistries and CBC reported out same day. PEP run Monday and Thursday with results out same day. If a microtainer (m-GrnTT or m-LTT) with whole blood is not submitted, WBC is estimated from the blood smear

36 Avian/Reptile CBC

Methodology: Manual

Specimen Requirements Air dried Peripheral Blood smears and 0.25 mL whole blood, m-LTT/m-GrnTT. (Minimum: 30 µL whole blood)Heparin preferred for some species (e.g. Crows, Jays)

Reported Time Same Day

Collection Notes It is always best to submit well-prepared, fresh, air-dried peripheral blood smears with your blood sample to prevent cell lysis and clumping. Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: If a microtainer is submitted, a WBC count and estimate from the slide made at the lab will be done. If only peripheral blood smears were submitted, a WBC estimate will be done from review of the peripheral blood smears.

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1036 Avian/Reptile CBC, Follow-Up

Methodology: Manual

Specimen Requirements Air dried Peripheral Blood smears and 0.25 mL whole blood, m-LTT/m-GrnTT. (Minimum: 30 µL whole blood)Heparin preferred for some species (e.g. Crows, Jays)

Reported Time Same Day

Collection Notes It is always best to submit well-prepared, fresh, air-dried peripheral blood smears with your blood sample to prevent cell lysis and cell clumping.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: If a microtainer is submitted, a WBC count and estimate from the slide made here will be done, if you send peripheral blood smears, a WBC estimate will be done from review of the peripheral blood smears. The WBC count that looks most accurate will be reported.Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

1530 Avian/Reptile, Giardia/Crypto w/ Modified Acid Fast

Giardia/Cryptosporidium (IFA)

Specimen Requirements 3-5 grams Fresh Feces, Fecal Tube

Reported Time Daily AM - Same Day

Modified Acid Fast Stain

Collection Notes None

Additional Comments: Screening for Giardia and Cryptosporidium in avian and non-mammalian species.To rule out Giardia and Cryptosporidia in mammalian species, see Giardia/Cryptosporidia IFA #240

1188 Reptile Mini Liver Panel

Methodology: Spectrophotometry

Total ProteinAlbumin

Specimen Requirements 0.1 mL Serum, m-SST/m-RTT Heparinized plasma or 0.25 mL whole blood from m-GrnTT is also accepted.Please call our Client Services for information about minimums on short samples.

Reported Time Same Day

CK (Creatine Kinase)Bile Acids

AST (Aspartate Transaminase)GGT (Gamma-glutamyltransferase)

Collection Notes None

Additional Comments: None

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5026 Reptile Mini Renal Panel

Methodology: Spectrophotometry

Uric AcidCa/Phos Ratio

Specimen Requirements 0.1 mL Serum, m-SST/m-RTT Heparinized plasma or 0.25 mL whole blood from m-GrnTT is also accepted.Please call our Client Services for information about minimums on short samples.

Reported Time Same Day

Phosphorous Calcium

Collection Notes None

Additional Comments: None

87 Reptile Profile

WBC Est/DiffHematocrit (HCT)GlucoseCalcium

Specimen Requirements Chemistry: 0.3 mL (minimum: 100 µL), heparinized plasma or 250 µL Whole BloodCBC: 2 air dried slides and 0.1 mL (Absolute minimum: 30 µL) of either heparinized or EDTA whole blood. Heparin is preferred for most species.Please call our Client Services for information about minimums on short samples.

Reported Time Same Day

PhosphorusBile AcidTotal ProteinCholesterol

Uric AcidCa/Phos RatioAST (Aspartate Transaminase)

Collection Notes One m-GrnTT can be used, volume allowing. Submit m-GrnTT without centrifugation in order for HCT and chemistries to be done an same tube. Prepare slides at clinic.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: If a microtainer (M-GrnTT or M-LTT) is not submitted, WBC is estimated from the blood smear.

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88 Reptile Profile, Follow-up

WBC Est/DiffHematocrit (HCT)GlucoseCalcium

Specimen Requirements Chemistry: 0.3 mL (minimum: 100 µL), heparinized plasma or 250 µL Whole BloodCBC: 2 air dried slides and 0.1 mL (Absolute minimum: 30 µL) of either heparinized or EDTA whole blood. Heparin is preferred for most species.Please call our Client Services for information about minimums on short samples.

Reported Time Same Day

PhosphorusBile AcidTotal ProteinCholesterol

Uric AcidCa/Phos RatioAST (Aspartate Transaminase)

Collection Notes One m-GrnTT can be used, volume allowing. Submit m-GrnTT without centrifugation in order for HCT and chemistries to be done an same tube. Prepare slides at clinic.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: If a microtainer (M-GrnTT or M-LTT) is not submitted, WBC is estimated from the blood smear.

Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

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Small Mammal Panels

55 CBC, Exotic/Small Mammal

Methodology: Manual

Specimen Requirements Hematocrit and 3 air dried slides.

Reported Time Same Day

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Includes Hct, WBC estimate, and differential. Used for "short" sample specimens.

1044 CBC, Platelet if Indicated

Methodology: Flow Cytometry

Specimen Requirements 0.5 mL Whole Blood, LTT

Reported Time Same Day

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: It is possible a platelet count cannot be performed due to clumping.

101004 Small Mammal Chem Screen II

Methodology: Spectrophotometry

GlucoseBUN (Blood Urea Nitrogen)CreatinineCalciumPhosphorusTotal ProteinBilirubin, Total

Specimen Requirements Chemistry: 1  m-SST or m-GrnTT Minimum: 250 uL serum/plasma OR 550 µL whole blood for non-ferrets (1 mL whole blood for ferrets).

Reported Time Same Day

AST (Aspartate Transaminase)CK (Creatine Kinase)CholesterolAmylaseLipaseBile Acids

AlbuminGlobulinA/G RatioALP (Alkaline Phosphatase)GGT (Gamma-glutamyltransferase)ALT (Alanine Transaminase)

Test Alias: Ferret/Small Mammal 20-Chem

Collection Notes Please call our Client Services for information about minimums on short samples.

Additional Comments: Chemistry screen does NOT includes: electrolytes, carbon dioxide, osmolality, or anion gap.

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956 Small Mammal Chemistry Screen I

Methodology: Spectrophotometry

GlucoseBUN (Blood Urea Nitrogen)CreatinineCalcium

Specimen Requirements 0.3 mL, m-SST/m-RTT, (Minimum: 150 µL serum) Heparinized plasma or 350 µL whole blood for non-ferrets(0.6 mL whole blood for ferrets) m-GrnTT OKPlease call our Client Services for information about minimums on short samples.

Reported Time Same Day

PhosphorusTotal ProteinAlbuminGlobulin

A/G RatioALT (Alanine Transaminase)AST (Aspartate Transaminase)

Test Alias: Ferret/Small Mammal 12-Chem Panel

Collection Notes None

Additional Comments: None

101002 Small Mammal Comprehensive Chemistry Screen

Methodology: Spectrophotometry

GlucoseBUN (Blood Urea Nitrogen)CreatinineSodiumPotassiumSodium/Potassium RatioChlorideCarbon Dioxide (CO2)Anion Gap

Specimen Requirements 2  m-SST or m-GrnTT, Minimum: 300 uL serum/plasma OR 650 µL whole blood for non-ferrets (1 mL whole blood for ferrets).

Reported Time Same Day

CalciumPhosphorusCalculated OsmolalityTotal ProteinAlbuminGlobulinAlbumin/GlobulinBilirubin, Total

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)AST (Aspartate Transaminase)GGT (Gamma-glutamyltransferase)CholesterolCK (Creatine Kinase)AmylaseLipaseBile Acids

Test Alias: Ferret/Small Mammal 27-Chem

Collection Notes Please call our Client Services for information about minimums on short samples.

Additional Comments: None

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1002 Small Mammal Comprehensive Panel

CBCGlucoseBUN (Blood Urea Nitrogen)CreatinineSodiumPotassiumSodium/Potassium RatioChlorideCarbon Dioxide (CO2)Anion Gap

Specimen Requirements Chemistry: 2  m-SST or m-GrnTT, Minimum: 300 uL serum/plasma OR 650 µL whole blood for non-ferrets (1 mL whole blood for ferrets).CBC: 2 air dried slides, and 1 m-LTT or m-GrnTT Minimum: 250 uL  whole blood

Reported Time Same Day

CalciumPhosphorusCalculated OsmolalityTotal ProteinAlbuminGlobulinAlbumin/GlobulinBilirubin, Total

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)AST (Aspartate Transaminase)GGT (Gamma-glutamyltransferase)CholesterolCK (Creatine Kinase)AmylaseLipaseBile Acids

Test Alias: Ferret/Small Mammal 27-Chem w/CBC

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Please call our Client Services for information about minimums on short samples.

Additional Comments: If a microtainer (m-GrnTT or m-LTT) is not submitted, WBC is estimated from the blood smear.

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10022 Small Mammal Comprehensive Panel w/ Protein Electrophoresis

CBCPEP (Protein Electrophoresis)GlucoseBUN (Blood Urea Nitrogen)CreatinineSodiumPotassiumSodium/Potassium RatioChlorideCarbon Dioxide (CO2)Anion Gap

Specimen Requirements Chemistry: 2  m-SST or m-GrnTT Minimum: 300 uL serum or plasma OR 650 µL whole blood for non-ferrets (1 mL whole blood for ferrets).CBC: 2 air dried slides, and 1 m-LTT or m-GrnTT Minimum: 250 uL  whole blood for CBC

Reported Time Same Day, PEP Mon/Thur Same Day

CalciumPhosphorusCalculated OsmolalityTotal ProteinAlbuminGlobulinAlbumin/GlobulinBilirubin, Total

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)AST (Aspartate Transaminase)GGT (Gamma-glutamyltransferase)CholesterolCK (Creatine Kinase)AmylaseLipaseBile Acids

Test Alias: Ferret/Small Mammal 27-Chem w/CBC and PEP

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Please call our Client Services for information about minimums on short samples.

Additional Comments: If a microtainer (m-GrnTT or m-LTT) is not submitted, WBC is estimated from the blood smear. Protein Electrophoresis is run Monday and Thursdays, with results out same day

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11002 Small Mammal Comprehensive Panel, Follow-Up

CBCGlucoseBUN (Blood Urea Nitrogen)CreatinineSodiumPotassiumSodium/Potassium RatioChlorideCarbon Dioxide (CO2)Anion Gap

Specimen Requirements Chemistry: 2  m-SST or m-GrnTT, Minimum: 300 uL serum/plasma OR 650 µL whole blood for non-ferrets (1 mL whole blood for ferrets).CBC: 2 air dried slides, and 1 m-LTT or m-GrnTT Minimum: 250 uL  whole blood

Reported Time Same Day

CalciumPhosphorusCalculated OsmolalityTotal ProteinAlbuminGlobulinAlbumin/GlobulinBilirubin, Total

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)AST (Aspartate Transaminase)GGT (Gamma-glutamyltransferase)CholesterolCK (Creatine Kinase)AmylaseLipaseBile Acids

Test Alias: Ferret/Small Mammal 27-Chem/CBC, Follow-up

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Please call our Client Services for information about minimums on short samples.

Additional Comments: If a microtainer (m-GrnTT or m-LTT) is not submitted, WBC is estimated from the blood smear.

Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

1186 Small Mammal Mini Liver Panel

Methodology: Spectrophotometry

CK (Creatine Kinase)Bilirubin Total

Specimen Requirements 0.15 mL Serum m-SST/m-RTT. Heparinized plasma or 0.35 mL whole blood (0.6 mL for ferrets) from m-GrnTT is also accepted.

Reported Time Same Day

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)

AST (Aspartate Transaminase)GGT (Gamma-glutamyltransferase)

Collection Notes Please call our Client Services for information about minimums on short samples.

Additional Comments: None

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5028 Small Mammal Mini Renal Panel

Methodology: Spectrophotometry

CreatinineBUN (Blood Urea Nitrogen)Sodium

Specimen Requirements 0.15 mL Serum, m-SST/m-RTT. Heparinized plasma or 0.35 mL whole blood (0.6 mL for ferrets) from m-GrnTT is also accepted.

Reported Time Same Day

ChloridePotassiumCalcium

PhosphorousTotal ProteinAlbumin

Collection Notes Please call our Client Services for information about minimums on short samples.

Additional Comments: None

56 Small Mammal Panel I

CBCGlucoseBUN (Blood Urea Nitrogen)CreatinineCalcium

Specimen Requirements 0.3 mL, m-SST/m-RTT, (Minimum: 150 µL serum), Heparinized plasma or 350 µL whole blood for non-ferrets(0.6 mL whole blood for ferrets) m-GrnTT OKCBC: Minimum: 250 uL EDTA whole blood (heparinized whole blood OK), 2 air dried slides.Please call our Client Services for information about minimums on short samples.

Reported Time Same Day

PhosphorusTotal ProteinAlbuminGlobulin

A/G RatioALT (Alanine Transaminase)AST (Aspartate Transaminase)

Test Alias: Ferret/Small Mammal 12-Chem w/CBC

Collection Notes Please call our Client Services for information about minimums on short samples.

Additional Comments: If a microtainer (m-GrnTT or m-LTT) is not submitted, WBC is estimated from the blood smear.

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84 Small Mammal Panel I, Follow-Up

CBCGlucoseBUN (Blood Urea Nitrogen)Creatinine

Specimen Requirements Chemistry: 0.3 mL, m-SST/m-RTT, Minimum: 150 uL serum (heparinized plasma OK) or 350 µL whole blood for non-ferrets, 0.6 mL whole blood for ferrets.CBC: Minimum: 250 uL EDTA whole blood (heparinized whole blood OK), 2 air dried slides.Please call our Client Services for information about minimums on short samples.

Reported Time Same Day

CalciumPhosphorusTotal ProteinAlbumin

GlobulinA/G RatioALT (Alanine Transaminase)AST (Aspartate Transaminase)

Ferret/Small Mammal 12-Chem/CBC, Follow-up

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Please call our Client Services for information about minimums on short samples.

Additional Comments: If a microtainer (M-GrnTT or M-LTT) is not submitted, WBC is estimated from the blood smear. Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

1004 Small Mammal Panel II

CBCGlucoseBUN (Blood Urea Nitrogen)CreatinineCalciumPhosphorusBilirubin, Total

Specimen Requirements Chemistry: 1  m-SST or m-GrnTT Minimum: 250 uL serum/plasma OR 550 µL whole blood for non-ferrets (1 mL whole blood for ferrets).CBC: 2 air dried slides, and 1 m-LTT or m-GrnTT Minimum: 250 uL  whole blood

Reported Time Same Day

AST (Aspartate Transaminase)CK (Creatine Kinase)CholesterolAmylaseLipaseBile Acids

Total ProteinAlbuminGlobulinA/G RatioALP (Alkaline Phosphatase)GGT (Gamma-glutamyltransferase)ALT (Alanine Transaminase)

Test Alias: Ferret/Small Mammal 20-Chem w/CBC

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Please call our Client Services for information about minimums on short samples.

Additional Comments: Panel does NOT includes: electrolytes, carbon dioxide, osmolality, or anion gap. If a microtainer (m-GrnTT or m-LTT) is not submitted, WBC is estimated from the blood smear.

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10044 Small Mammal Panel II w/ Protein Electrophoresis

CBCPEP (Protein Electrophoresis)GlucoseBUN (Blood Urea Nitrogen)CreatinineCalciumPhosphorus

Specimen Requirements Chemistry: 1  m-SST or m-GrnTT Minimum: 250 uL serum/plasma OR 550 µL whole blood for non-ferrets (1 mL whole blood for ferrets).CBC: 2 air dried slides, and 1 m-LTT or m-GrnTT Minimum: 250 uL  whole blood

Reported Time Same Day

AST (Aspartate Transaminase)CK (Creatine Kinase)CholesterolAmylaseLipaseBile AcidsBilirubin, Total

Total ProteinAlbuminGlobulinA/G RatioALP (Alkaline Phosphatase)GGT (Gamma-glutamyltransferase)ALT (Alanine Transaminase)

Test Alias: Ferret/Small Mammal 20-Chem w/CBC & PEP

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Please call our Client Services for information about minimums on short samples.

Additional Comments: Panel does NOT includes: electrolytes, carbon dioxide, osmolality, or anion gap. If a microtainer (m-GrnTT or m-LTT) is not submitted, WBC is estimated from the blood smear. Protein electrophoresis is run Monday and Thursday, with results out same day.

11004 Small Mammal Panel II, Follow-Up

CBCGlucoseBUN (Blood Urea Nitrogen)CreatinineCalciumPhosphorusBilirubin, Total

Specimen Requirements Chemistry: 1  m-SST or m-GrnTT Minimum: 250 uL serum/plasma OR 550 µL whole blood for non-ferrets (1 mL whole blood for ferrets).CBC: 2 air dried slides, and 1 m-LTT or m-GrnTT Minimum: 250 uL  whole blood

Reported Time Same Day

AST (Aspartate Transaminase)CK (Creatine Kinase)CholesterolAmylaseLipaseBile Acids

Total ProteinAlbuminGlobulinA/G RatioALP (Alkaline Phosphatase)GGT (Gamma-glutamyltransferase)ALT (Alanine Transaminase

Test Alias: Ferret/Small Mammal 20-Chem/CBC, Follow-up

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Please call our Client Services for information about minimums on short samples.

Additional Comments: Panel does NOT includes: electrolytes, carbon dioxide, osmolality, or anion gap. If a microtainer (m-GrnTT or m-LTT) is not submitted, WBC is estimated from the blood smear.

Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

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Cytology Submission Guidelines

Complete history is essential for accurate results– including location of lesion, previous lab accession numbers, clinical signs, and treatment. Label all samples with patient name and sample site. Indicate method of collection on the submission form (e.g. wash/lavage, fine needle, tissue impression). If culture or other testing requiring special handling is needed on a cytology submission, please alert the laboratory on the requisition form and provide any necessary instruction.

Packaging and Transportation When preparing cytology samples for transport, thoroughly air dry the slides before inserting into slide holder and do not refrigerate the slides. Also, ensure any fluid caps are secure. Formalin can have significant effects, causing rapid cell fixation and disintegration, limiting stain uptake with the stains used for cytologic evaluation. If submitting a sample for histology at the same time, package them separately and make a note on the requisition form. Even exposure to formalin fumes can rapidly deteriorate cells, so it is critical that formalin is kept far away from cytology samples.

Reporting Options While both the Mini report and Full report provide a microscopic diagnosis and comments, a Full report additionally provides a microscopic description.

Sample Collection Fine Needle Aspirate Protocol: 1. If the cytology is a fine needle aspirate, use a 21g. -22 g. needle (smaller needles increase cell rupture, larger needles increase hemodilution) and 3-12 mL syringe depending on material/tissue aspirated. The softer the tissue, the smaller the syringe and needle necessary for aspiration. NOTE: Lymph nodes often do not require suction as this increases the chance of rupture. 2. Insert the needle into the mass (avoid the center of larger lesions as they are apt to be necrotic). In order to get the most representative sample the needle should be directed in at least 3 different directions with suction applied. For large masses, different aspects of the mass should be sampled; notation of site of aspiration on the submission form, slides, and your medical records can be helpful to guide any future surgical biopsy that may be needed. 3. Release the negative pressure slowly and withdraw the needle from the mass. 4. Remove the needle, from syringe, fill the syringe with air, replace the needle and force the cellular material onto a slide or slides. 5. Cover the material on the slide with another slide, allow the material to spread and then horizontally pull the slides apart without pressure. This must be done quickly to avoid clot formation. 6. Quickly air dry the slides. Do not heat fix. Try to make 3 - 5 slides from each lesion. Do not package the slides for transport until thoroughly dried. Do not refrigerate slides. For additional collection protocols, see the collection notes section for desired test.

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Selecting the Correct Test

See the explanations below to help select the correct test for the sample provided: Cytology, Full (318) – Can be performed on submitted slides, washes, mass aspirate fluid, urine, vaginal swabs, or other specimens. Multiple lymph nodes are charged as one site. Cytology, Additional Site (318x_) – Additional sites submitted together

Cytology, Mini (5318) – Can be performed on submitted slides. Multiple lymph nodes are charged as one site. Cytology, Mini, Additional Site (5318x_) – Additional sites submitted together.

Body Cavity or Joint Fluid Cytology (14) – Includes nucleated cell count. Recommended test for paracentesis (abdominal, paracardial, or thoracic) or joint fluid. Cannot be performed on slides only. Fluid Cytology, Additional Site (3014) – Additional sites submitted together.

Buffy Coat Cytology (1318) – This is the most sensitive method to look for circulating mast cells in the blood. Test performed on buffy coat smears prepared from submitted LTT.

Bone Marrow Cytology (304) – Any bone which contains red marrow can be used as a site for aspiration or core biopsy; note that for long bones (e.g. humerus) in adult animals, red marrow is found at the ends of the bones (epiphysis/metaphysis), with minimal hematopoietic cells in the shaft/diaphysis. Evaluation of a CBC collected on the same day as the marrow aspirate is essential for an accurate interpretation of the marrow findings. Bone Marrow Cytology w/ CBC (364) – CBC results included with Bone Marrow Cytology.

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Cytology

14 Body Cavity/Joint Fluid Cytology

Specimen Requirements 1.0 mL Body Cavity or Joint Fluid, LTT required, RTT/CTT recommended for possible culture. Air dried slides also recommended.Smaller volumes may be sufficient for a count for joints.

Reported Time Reviewed M-Sat on Days

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Please include clinical history with sample. Descriptive report contains nucleated cell count, specific gravity, total protein, and cytology. For cytology on fluids from other sources (i.e. masses) order Cytology 318

3014 Body Cavity/Joint Fluid Cytology, Additional Source

Specimen Requirements 1.0 mL Body Cavity or Joint Fluid, LTT required, RTT/CTT recommended for possible culture. Air dried slides also desired.Smaller volumes may be sufficient for a count for joints.

Reported Time Reviewed M-Sat on Days

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Please include clinical history with sample.Descriptive report contains nucleated cell count, specific gravity, total protein, and cytology. For cytology on fluids from other sources (i.e. masses) please order Cytology 318

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304 Bone Marrow Cytology

Specimen Requirements 4 - 6 air dried, unstained thin smears of bone marrow.

Reported Time Reviewed M-Sat on Days

Collection Notes Please THOROUGHLY DRY slides before closing transport container and do not refrigerate.Protocol:1. Any bone which contains red marrow can be used as a site for aspiration or core biopsy. For small animals, collection sites include proximal femur, humerus, and iliac crest. For adult large animals, collection sites include the sternum, ribs and ilium (tuber coxae).2. The bone marrow aspiration site should be aseptically prepared and a local anesthetic given.3. Using a bone marrow biopsy needle and stylet, go through the cortical bone usually 0.5 - 1 cm deep.4. Remove the stylet and gently aspirate the specimen. As soon as marrow fills the hub of the syringe, release the plunger and remove the syringe. Collect 2-4 drops of marrow (0.2 mL -0.25 mL).ASPIRATION OF A LARGER VOLUME USUALLY RESULTS IN EXCESSIVE CONTAMINATION OF THE MARROW WITH PERIPHERAL BLOOD.5. Marrow material is expelled from the syringe onto a glass slide held at an angle of 45 degrees. Spicules will tend to stick to the slide and contaminating blood will run off.6. Smears are prepared by gentle squash technique. Slides are pulled apart quickly lengthwise from each other. As for FNA from solid tissue, let the weight of the top slide spread the bone spicules. DO NOT PRESS SLIDES TOGETHER. ALLOW SLIDES TO AIR DRY. Using this technique, make at least 4 slides. Do not refrigerate the marrow slides.7. Any remaining material in the syringe as well as what remains in the needle should be expelled into a small EDTA tube.

Additional Comments: EVALUATION OF A CBC COLLECTED ON THE SAME DAY AS THE MARROW ASPIRATE IS ABSOLUTELY ESSENTIAL FOR AN ACCURATE INTERPRETATION OF THE MARROW FINDINGS.

To include a CBC, order Bone Marrow and CBC 364;and see test for CBC requirements.

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364 Bone Marrow Cytology W/ CBC

Bone Marrow Cytology

Specimen Requirements Cytology: 4 - 6 air dried, unstained thin smears of bone marrowCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Reviewed M-Sat on Days

CBC

Collection Notes Please THOROUGHLY DRY slides before closing transport container and do not refrigerate.Protocol:1. Any bone which contains red marrow can be used as a site for aspiration or core biopsy. For small animals, collection sites include proximal femur, humerus, and iliac crest. For adult large animals, collection sites include the sternum, ribs and ilium (tuber coxae).2. The bone marrow aspiration site should be aseptically prepared and a local anesthetic given.3. Using a bone marrow biopsy needle and stylet, go through the cortical bone usually 0.5 - 1 cm deep.4. Remove the stylet and gently aspirate the specimen. As soon as marrow fills the hub of the syringe, release the plunger and remove the syringe. Collect 2-4 drops of marrow (0.2 mL -0.25 mL). ASPIRATION OF A LARGER VOLUME USUALLY RESULTS IN EXCESSIVE CONTAMINATION OF THE MARROW WITH PERIPHERAL BLOOD.5. Marrow material is expelled from the syringe onto a glass slide held at an angle of 45 degrees. Spicules will tend to stick to the slide and contaminating blood will run off.6. Smears are prepared by gentle squash technique. Slides are pulled apart quickly lengthwise from each other. As for FNA from solid tissue, let the weight of the top slide spread the bone spicules. DO NOT PRESS SLIDES TOGETHER. ALLOW SLIDES TO AIR DRY. Using this technique, make at least 4 slides. Do not refrigerate the marrow slides.7. Any remaining material in the syringe as well as what remains in the needle should be expelled into a small EDTA tube.

Additional Comments: Please include a complete history.

For Bone Marrow Cytology only, refer to test code 304.

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1318 Buffy Coat Cytology

Specimen Requirements 1.0 mL Whole Blood, LTT

Reported Time Reviewed M-Sat on Days

Collection Notes None

Additional Comments: The buffy coat sample is prepared by centrifuging a whole blood sample from an EDTA Tube (LTT). The layer between the plasma and the RBCs is called the buffy coat and is examined by the Pathologist. This is the most sensitive method to look for mast cells.

29 CSF Analysis

Specimen Requirements 0.5 mL CSF LTT and RTT/CTT, Minimum 0.2mL in each tube.

Reported Time Reviewed M-Sat on Days

Collection Notes Protocol: 1. To minimize blood contamination, it is recommended to allow the CSF to drip into a small EDTA tube. 2. A CTT is recommended for chemistries and culture.

Additional Comments: All CSF specimens receive nucleated cell count, RBC count, glucose, microprotein, and cytology. We request 1 mL total, but will work with any volume. If only a small amount of CSF is collected (<0.5 mL), please prioritize desired testing. EDTA (LTT) inhibits bacterial growth and can not be used for culture.

1029 CSF Analysis, Additional Site

Specimen Requirements 0.5 mL CSF LTT and CTT, Minimum 0.2mL in each tube.

Reported Time Reviewed M-Sat on Days

Collection Notes Protocol: 1. To minimize blood contamination, it is recommended to allow the CSF to drip into a small EDTA tube.2. A CTT is recommended for chemistries and culture.

Additional Comments: All CSF specimens receive nucleated cell count, RBC count, glucose, microprotein, an cytology. We request 1 mL total, but will work with any volume. If only a small amount of CSF is collected <0.5 mL, please prioritize testing desired. EDTA (LTT) inhibits bacterial growth and can not be used for culture.

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51 CSF Panel, Special

CSF Analysis

Specimen Requirements 0.5 mL each LTT and/or RTT/CTT. Minimum: 0.4 mL total

Reported Time Reviewed M-Sat on days

CSF CK (Creatine Kinase) Pandy's Test

Collection Notes Protocol: 1. To minimize blood contamination, it is recommended to allow the CSF to drip into a small EDTA tube. 2. A CTT is recommended for chemistries and culture.

Additional Comments: Includes CSF panel 29, as well as CK value and Pandy's test. We request 1 mL total, but will work with any volume.If only a small amount of CSF is collected (<0.5 mL), please prioritize desired tests. EDTA (LTT) inhibits bacterial growth and cannot be used for culture.

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318 Cytology

Specimen Requirements 3-5 air dried slides or tissue imprints, unstained.Remaining fluid may be submitted as well in LTT and/or CTT at no additional cost. For cytology on trans/endotracheal wash, fluid in LTT and CTT recommended.

Reported Time Reviewed M-Sat on Days

Collection Notes FINE NEEDLE ASPIRATE PROTOCOL:1. If the cytology is a fine needle aspirate, use a 21g. -22 g. needle (smaller needles increase cell rupture) and 3-12 mL syringe depending on material/tissue aspirated. The softer the tissue, the smaller the syringe and needle necessary for aspiration. NOTE: Lymph nodes often do not require suction as this increases chance of rupture.2. Insert the needle into the mass (avoid the center of larger lesions as they are apt to be necrotic). In order to get the most representative sample the needle should be directed in at least 3 different directions with suction applied.3. Release the negative pressure slowly and withdraw the needle from the mass.4. Remove the needle,from syringe, fill the syringe with air, replace the needle and force the cellular material onto a slide or slides.5. Cover the material on the slide with another slide, allow the material to spread and then horizontally pull the slides apart without pressure. This must be done quickly to avoid clot formation.6. Quickly air dry the slides. DO NOT HEAT FIX. Try to make 3 - 5 slides from each lesion. Do not package the slides for transport until thoroughly dried. Do not refrigerate slides.

TISSUE IMPRESSION PROTOCOL:1. Impression smears can be made from an excised mass using the TOUCH IMPRINT TECHNIQUE. Blot tissue onto paper towel, then onto slides.2. Quickly air dry the slides. DO NOT HEAT FIX.3. Do not package the slides for transport until thoroughly dried. Do not refrigerate slides. Note that impression smears of ulcerated, dermal lesions are often of low diagnostic utility. VAGINAL CYTOLOGY FOR ESTRUS PROTOCOL:1. Insert the cotton tip swab against the caudodorsal surface of the vaginal vault (avoid the vulva and clitoral fossa) and advance the swab in a craniodorsal direction until it passes over the ischial arch. The swab should reach the pelvic canal.2. Rotate the swab 360 degrees in each direction and withdraw.3. For optimal exfoliation, gently roll the swab across the surface of slide, DO NOT SMEAR.4. Dry thoroughly before placing in slide container. Do not refrigerate slides.

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Additional Comments: Complete history and location of lesion are essential for accurate results. Multiple lymph nodes are charged as one site. If the cytology is a paracentesis (abdominal, paracardial, or thoracic) or joint fluid with submission of fluid, refer to test Fluid Cytology (Body Cavity/ Joint Fluid) 14. Note that impression smears of ulcerated, dermal lesions are often of low diagnostic utility. EDTA (LTT) inhibits bacterial growth. If culture is required, use plain CTT or sterile transport tube.

Test includes microscopic description, diagnosis, and comments.Price is for a single site. Every site after is an additional $26.10.

421 Cytology Slide Prep Only

Specimen Requirements Requires fluid or other cytology sample, LTT/CTT

Reported Time Varies

Collection Notes None

Additional Comments: This charge is for preparing cytology slides from samples submitted only. Slides will be sent on to destination provided (additional shipping charges may apply).To have a cytology performed, order Cytology 318.

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5318 Mini-Cytology

Specimen Requirements 3-5 air dried slides, unstained or tissue imprints

Reported Time Reviewed M-Sat on Days

Collection Notes FINE NEEDLE Aspirate PROTOCOL:1. If the cytology is a fine needle aspirate, use a 21g. -22 g. needle (smaller needles increase cell rupture) and 3-12 mL syringe depending on material/tissue aspirated. The softer the tissue, the smaller the syringe and needle necessary for aspiration. NOTE: Lymph nodes often do not require suction as this increases chance of rupture.2. Insert the needle into the mass (avoid the center of larger lesions as they are apt to be necrotic). In order to get the most representative sample the needle should be directed in at least 3 different directions with suction applied.3. Release the negative pressure slowly and withdraw the needle from the mass.4. Remove the needle,from syringe, fill the syringe with air, replace the needle and force the cellular material onto a slide or slides.5. Cover the material on the slide with another slide, allow the material to spread and then horizontally pull the slides apart without pressure. This must be done quickly to avoid clot formation.6. Quickly air dry the slides. DO NOT HEAT FIX. Try to make 3 - 5 slides from each lesion. Do not package the slides for transport until thoroughly dried. Do not refrigerate slides.

TISSUE IMPRESSION PROTOCOL:1. Impression smears can be made from an excised mass using the TOUCH IMPRINT TECHNIQUE. Blot tissue onto paper towel, then onto slides.2. Quickly air dry the slides. DO NOT HEAT FIX.3. Do not package the slides for transport until thoroughly dried. Do not refrigerate slides. Note that impression smears of ulcerated, dermal lesions are often of low diagnostic utility. VAGINAL CYTOLOGY FOR ESTRUS PROTOCOL:1. Insert the cotton tip swab against the caudodorsal surface of the vaginal vault (avoid the vulva and clitoral fossa) and advance the swab in a craniodorsal direction until it passes over the ischial arch. The swab should reach the pelvic canal.2. Rotate the swab 360 degrees in each direction and withdraw.3. For optimal exfoliation, gently roll the swab across the surface of slide, DO NOT SMEAR.4. Dry thoroughly before placing in slide container. Do not refrigerate slides.

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Additional Comments: Complete history and location of lesion are essential for accurate results. Multiple lymph nodes are charged as one site. If the cytology is a paracentesis (abdominal, paracardial, or thoracic) or joint fluid with submission of fluid, refer to test Fluid Cytology (Body Cavity/ Joint Fluid) 14. Note that impression smears of ulcerated, dermal lesions are often of low diagnostic utility. EDTA (LTT) inhibits bacterial growth. If culture is required, use plain CTT or sterile transport tube.Reports will provide diagnosis and comments sections but will not contain a detailed microscopic description.

Price is for a single site. Every site after is an additional $21.00.

32 Semen Evaluation

Specimen Requirements 1.0 mL Semen, LTT/CTT

Reported Time Reviewed M-Sat on Days

Collection Notes For canines only: Collect entire ejaculate for accurate reporting of sperm total per ejaculate.

Additional Comments: Includes sperm count, pH and cytology.Please note that sperm motility should be assessed immediately after sample collection and will not be assessed with this cytology.

***For Canines, Equines, and Bovines only.***

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Endocrinology

697 ACTH Plasma, Endogenous

Methodology: Chemiluminescent Immunoassa

Specimen Requirements 0.5 mL EDTA Plasma, Separated and Frozen, CTT

Reported Time Mon/Wed/Fri, Same day

Collection Notes Spin down LTT immediately and separate from cells. Freeze as soon as possible. Frozen plasma stable for 14 days. Avoid grossly hemolyzed and/or lipemic samples

Additional Comments: Dexamethasone suppression test is no longer recommended as the primary test for diagnosis of PPID/Cushing's Disease in horses.  Endogenous ACTH is the preferred test.  Also see test code 6971 for information about TRH stimulation testing.

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39 ACTH Response

Methodology: Chemiluminescent Immunoassa

Pre Cortisol

Specimen Requirements 0.5 mL Serum each RTTx2; Pre, 1 hour Post. DO NOT USE SST TO COLLECT OR SPIN DOWN SAMPLE

Reported Time Same Day

Post Cortisol

Collection Notes PROTOCOL:1.  Collect a resting sample in a RTT, 0.5 mL of serum is required.  Separate serum if possible and refrigerate. 2.  Inject 0.25 mg (250 µg) of Cortrosyn IM or IV (1 vial). 3.  Collect a second sample EXACTLY 1 HOUR post injection in RTT, label clearly as to the collection status (post), and preserve in the same manner as the first sample.

NOTE: The test has been validated using Cortrosyn. Although compounded gels are available, they vary greatly in peak times and are not recommended.

ALTERNATE CANINE PROTOCOL (Microdose): In order to keep Cortrosyn costs down, use the specific dose of 5 ug/kg IV - up to the maximum dose of 250 ug (one vial) per dog.  Whatever is left over can then be split into aliquots of 25 ug  or 50 ug using small plastic syringes.  Normally 1 mL of sterile physiologic saline is used as diluent.  If so, 25 ug would be 100 uL or 0.1 mL. ***When the patient is receiving Trilostane/Vetoryl, the timing of the test is important.  The pre-cortisol sample should be drawn 3-5 hours after the morning capsule.  The test is a one hour test, so in other words, the ACTH Response test should be completed 4-6 hours after the medication, which is usually given once a day in the morning.

Additional Comments: Pre and Post sample required. In addition to diagnosis of naturally occurring hyperadrenocorticism (Cushing's disease), this is the only diagnostic test for hypoadrenocorticism (Addison's disease), iatrogenic hyperadrenocorticism, and monitoring response to medical therapy of Cushing's disease. Prednisone, prednisolone, and other corticosteroids (except dexamethasone) will cross react. Recommend these drugs be discontinued at least 48 hrs before testing. If dog has been on prolonged steroids, patient must be tapered with the drug stopped for at least 3 weeks to let the adrenal gland recover. INTERPRETATION HYPERADRENOCORTICISM: An ACTH response test is done to CONFIRM clinical suspicion of hyperadrenocorticism. Stressful diseases (ill diabetics, renal failure, etc.) and current drug therapy may cause false positives. If the cortisol results are normal (and an adequate dose of Cortrosyn was administered) and there is high clinical suspicion of hyperadrenocorticism, sex hormone hyperadrenocorticism (atypical Cushing's) should be considered. Additional testing on the serum from this ACTH stimulation test can be performed. Please contact us within 7 days of this cortisol result for further testing options. A low-dose dexamethasone suppression test should also be considered, as some patients with Cushing's will be positive on one test but not on the other.INTERPRETATION HYPOADRENOCORTICISM: Pre and post cortisol <2 ug/dL are supportive of hypoadrenocorticism but need to be interpreted in light of other clinical findings and history. Failure to stimulate can occur with iatrogenic hyperadrenocorticism, dogs being treated for hyperadrenocorticism and Addison's disease.

For Feline Protocol See ACTH Response, Feline 1039

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1039 ACTH Response, Feline

Methodology: Chemiluminescent Immunoassa

Pre Cortisol

Specimen Requirements 3 RTT Minimum:  0.25 mL for each sampleAvoid severe lipemia

Reported Time Same Day

30 min Post Cortisol 60 min Post Cortisol

Collection Notes FELINE PROTOCOL: DO NOT USE SST TO COLLECT OR SPIN DOWN SAMPLE1.  Collect a resting sample in a RTT, 0.5 mL of serum is required.  Separate serum if possible and refrigerate. 2.  Inject 0.125 mg  of Cortrosyn  IM or IV, (1/2 vial). 3.  Collect a sample exactly 30 min and 60 min post injection in a RTT, label clearly as to the status (post-30, post-60), and preserve in the same manner as the first sample.

Additional Comments: Feline hyperadrenocorticism is very rare, we suggest consulting with an internist/clinical pathologist at the Laboratory prior to diagnostic testing. Dexamethasone suppression test is the favored diagnostic test for hyperadrenocorticism in cats. ACTH Response test is not recommended.NOTE: The test has been validated using Cortrosyn. Although compounded gels are available, they vary greatly in peak times and are not recommended. Prednisone, prednisolone, and other corticosteroids (except dexamethasone) will cross react. Recommend these drugs be discontinued at least 48 hrs before testing. If the patient has been on steroids >14 days, patient must be tapered and discontinue steroids for at least two weeks prior to testing.

57 Basic Feline

CBCChemistry Screen

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time See Comment

CK (Creatine Kinase)Total T4

FeLV (ELISA)FIV (IFA)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistries, CBC and FELV done same day.IFA testing done next day.In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

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92 Basic Health Profile

CBCUrinalysis

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smears; Urinalysis: 5 mL fresh urine

Reported Time Same Day

Chemistry Screen Total T4

Collection Notes Please indicate method of urine collection.Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Submit sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: None

1647 Canine Breeding Panel

Progesterone

Specimen Requirements Progesterone: 0.5 mL RTTCytology: 1-3 Vaginal Smears (Slides)

Reported Time Daily AM - Same Day

Cytology, Vaginal

Collection Notes Prepare the cytology sample by GENTLY rolling the swab over the slide. This technique will deliver optimal cellular material for review. Thoroughly dry the smears. DO NOT put slides into the slides holder until completely dry and do not refrigerate. Consistent labeling of tubes and smears: "Last Name, Owner, Animal, date" is very helpful when comparing serial results.Please allow all serum samples (RTT) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.Remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Timing of Sampling in Early Proestrus: 1) Progesterone should be monitored every 2-3 days to detect the preovulatory rise. 2) Vaginal Cytology should be evaluated every 2 days until >60% cornified. Rechecking: If progesterone is 1.0 - 3.0 ng/mL, consider confirming ovulation with serum progesterone concentration after at least 3 days. At ovulation, serum progesterone ranges from 3.8 - 10 ng/mL. Optimal breeding (fresh semen) is 2 days after ovulation.

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853 Chemistry Screen w/ T4 and Feline Viral II

Chemistry ScreenTotal T4

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time See Comment

FeLV (ELISA)FIV (IFA)

FCoV (FIP) Screen (IFA)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistry, and FeLV run same day, FIV/FIP run daily, AM. Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted. Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

2231 Cortisol, Resting

Methodology: Chemiluminescent Immunoassa

Specimen Requirements 0.5 mL Serum, RTT

Reported Time Same Day

Collection Notes If the patient has been on steroids for >14 days, patient should be taperdown and discontinue for at least 2 weeks prior to testing. If <14 days, discontinue for 38 hours prior to testing.DO NOT USE SST TO COLLECT OR SPIN DOWN SAMPLE

Additional Comments: A patient with a resting cortisol >2.0 ug/dL has a 99% chance of NOT having Addison's disease.With a resting cortisol of <2.0 ug/dL, an ACTH Response 39 is needed to rule-in Addison's disease. This interpretation is valid if no oral or topical steroids have been given in the last 6 weeks. A resting cortisol cannot be used to rule- in or out hyperadrenocorticism. A LDDS 38 and/or ACTH Response 39 needs to be performed.

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233 Cortisol, Urine

Methodology: Chemiluminescent Immunoassa

Specimen Requirements 0.5 mL Urine, Urine Tube/CTTIf sample is bloody or very cloudy/cellular, submit a larger volume (0.5 mL minimum) as the sample will need to be centrifuged.

Reported Time Same Day

Collection Notes None

Additional Comments: None

38 Dexamethasone Suppression

Methodology: Chemiluminescent Immunoassa

Pre Cortisol

Specimen Requirements 0.5 mL Serum each RTTx3; Pre, 4 hour, 8 hour post. DO NOT USE SST TO COLLECT OR SPIN DOWN SAMPLE

Reported Time Same Day

4 hr Post Cortisol 8 hr Post Cortisol

Collection Notes Collection Protocol:

Low Dose: NOTE: During the low dose dexamethasone suppression test, patient stress should be kept to a minimum. No other diagnostic procedures or grooming should be performed during the testing period.1. Collect 0.5 mL serum pre-injection. (RTT,CTT)2. Inject 0.01 mg/kg dexamethasone sodium phosphate IV.3. Collect 2 post samples at 3-4 and 8 hours post injection. (RTT,CTT) Centrifuge, separate serum into a sterile transport tube (RTT,CTT), and refrigerate or freeze until transport to the Lab.

High Dose:1. Collect 0.5 mL serum pre-injection. (RTT,CTT)2. Inject 0.1 mg/kg dexamethasone sodium phosphate IV.3. Collect 2 post samples at 4 and 8 hours post injection. Label each tube accordingly. (RTT,CTT) Centrifuge, separate serum into a sterile transport tube (RTT,CTT), and refrigerate or freeze until transport to the Lab. DO NOT FREEZE SPECIMENS IN GLASS TUBES.

Additional Comments: PLEASE NOTE: All three samples should be submitted together. A dexamethasone suppression is done to CONFIRM clinical suspicion of hyperadrenocorticism. Stressful diseases (ill diabetics, renal failure, etc.) and current drug therapy may cause false positives. Prednisone, prednisolone, and other corticosteroids (except dexamethasone) will cross react. Long term steroid therapy will suppress adrenal function. Recommend these drugs be discontinued at least 48 hrs before testing. Extra charge for more than 3 samples - please call the Laboratory for information. Up to 40% of pituitary dependent and adrenal dependent hyperadrenocorticoid dogs do not show suppression in LDDST. These dogs require HDDST, ACTH concentrations and/or abdominal imaging for further characterization.

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2038 Dexamethasone Suppression, Equine

Methodology: Chemiluminescent Immunoassa

Cortisol Pre

Specimen Requirements Pre and Post required, 0.5 mL (minimum 0.3mL) serum each, RTT ONLY

Reported Time Same Day

Cortisol 19-hr Post

Collection Notes PROTOCOL: DO NOT USE SST TO COLLECT OR SPINDOWN SAMPLE1.  Begin test between 4-6 PM. 2.  Draw a pre-injection blood sample into a RTT and label PRE. 3.  Inject 40 ug/kg of DEXAMETHASONE intramuscularly. 4.  Nineteen hours post-injection, draw a blood sample into a RTT and label POST-19.  5. Blood samples should be centrifuged, the serum separated, placed in a clean tube, and refrigerated or frozen until submitted to the Lab. 6.  Submit both samples at one time.

Additional Comments: This test is no longer recommended as the primary test for diagnosis of Pituitary Pars Intermedia Dysfunction (PPID)/Equine Cushing's Disease.  Please see test code 697 (ACTH, plasma) for additional information on testing for PPID. Normal horses: Cortisol </= 1.0 ug/dL 19 hours post dexamethasone. Interpret with clinical signs and rule out other conditions. Also, if performing this test in the fall, starting in September, interpret a failure to suppress with caution as false positives occasionally occur at that time of the year. Prednisone, prednisolone, and other corticosteroids (except dexamethasone) will cross react. Recommend these drugs be discontinued at least 24 hrs before testing.

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1008 Endocrine Panel

Chemistry Screen

Specimen Requirements 3 RTTs, Label each with draw time (0, 4, and 8 hour) 0.5 mL each ( minimum 0.1 mL for 4 and 8 hr posts).DO NOT USE SST TO COLLECT OR SPIN DOWN SAMPLE

Reported Time Same Day

Dexamethasone Suppression - 3 Cortisols

Collection Notes Collection Protocol: Low Dose: NOTE: During the low dose dexamethasone suppression test, patient stress should be kept to a minimum. No other diagnostic procedures or grooming should be performed during the testing period.1. Collect 0.5 mL serum pre-injection. (RTT,CTT)2. Inject 0.01 mg/kg dexamethasone, IV.3. Collect 2 post samples at 3-4 and 8 hours post injection. (RTT,CTT) Centrifuge, separate serum into a sterile transport tube (RTT,CTT), and refrigerate or freeze until transport to the Lab.

High Dose:1. Collect 0.5 mL serum pre-injection. (RTT,CTT)2. Inject 0.1 mg/kg dexamethasone, IV.3. Collect 2 post samples at 4 and 8 hours post injection. Label each tube accordingly. (RTT,CTT) Centrifuge, separate serum into a sterile transport tube (RTT,CTT), and refrigerate or freeze until transport to the Lab. DO NOT FREEZE SPECIMENS IN GLASS TUBES.

Additional Comments: PLEASE NOTE: All three samples should be submitted together. A dexamethasone suppression is done to CONFIRM clinical suspicion of hyperadrenocorticism. Stressful diseases (ill diabetics, renal failure, etc.) and current drug therapy may cause false positives. Prednisone, prednisolone, and other corticosteroids (except dexamethasone) will cross react. Long term steroid therapy will suppress adrenal function. Recommend these drugs be discontinued at least 48 hrs before testing. Extra charge for more than 3 samples - please call the Laboratory for information. Up to 40% of pituitary dependent and adrenal dependent hyperadrenocorticoid dogs do not show suppression in LDDST. These dogs require HDDST, ACTH concentrations and/or abdominal imaging for further characterization.

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8 Equine General Endocrine

Equine Chemistry Screen

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTTACTH Endo: 0.5 mL EDTA Plasma, Separated and Frozen, CTT.

Reported Time See Comments

ACTH Endogenous Total T4

Collection Notes For ACTH: Spin down LTT immediately and separate from cells. Freeze as soon as possible.Frozen plasma stable for 14 days. Avoid Grossly hemolyzed and/or lipemic samplesFor Chemistry: Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Dexamethasone suppression test is no longer recommended as the primary test for diagnosis of PPID/Cushing's Disease in horses.  Endogenous ACTH is the preferred test.  Chemistry Screen and Total T4 done same day.ACTH Endogenous run Mon/Wed/Fri with results out same day.Also see test code 6971 for information about TRH stimulation testing.

1634 Equine Insulin/ACTH, Endogenous

Insulin

Specimen Requirements Insulin: 0.5 mL serum, Frozen, RTT/CTTEndogenous ACTH: 1.0 mL EDTA Plasma, separated and frozen, CTT.

Reported Time Mon/Wed/Fri, Same day

Glucose Endogenous ACTH

Collection Notes For ACTH, Endo: Spin down LTT immediately and separate from cells. Freeze as soon as possible. Frozen plasma stable for 14 days. Avoid Grossly hemolyzed and/or lipemic samples.For Insulin: Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection; this limits hemolysis which can affect results. Separate serum into transport tube and freeze.Avoid lipemia and hemolysis.

Additional Comments: Report includes reference ranges and interpretation. Equine insulin and glucose levels have been suggested for diagnosing insulin dysregulation, but can be abnormal in any disease causing insulin resistance. For more information, please visit our website to view our test service update on Equine insulin testing.Testing not recommended on samples >24 hours old due to rapid deterioration.

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2404 Equine Senior Wellness I

CBCFibrinogenPlasma Protein

Specimen Requirements Chemistry/Insulin: 1.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.5 mL LTT and air dried peripheral blood smearsACTH Endo: 1.0 mL Frozen EDTA Plasma, CTT.Samples for Insulin testing CAN NOT be hemolyzed.

Reported Time See comment

InsulinGlucoseACTH Endogenous

Total T4Plasma Glucose

Collection Notes The LTT for ACTH Endo MUST be spun down within 30 minutes of collection and the plasma separated into a non-additive tube such as a CTT. Freeze until transported to lab. Insulin samples MUST be spun down within 30 minutes; if submission is to be delayed, draw a second tube or, volume allowing, pull some serum off the SST/RTT and Freeze for insulin testing.Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 30 minutes of collection; this then does not require collection of a GTT; this also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Equine insulin and glucose levels have been suggested for diagnosing insulin dysregulation, but can be abnormal in any disease causing insulin resistance. For more information, please visit our website to view our test service update on Equine insulin testing.Testing not recommended on samples >24 hours old due to rapid deterioration.

Chemistries and CBC reported out same day. ACTH Endo and Insulin run Monday/Wednesday/Friday, reported out same day.

Fibrinogen performed by heat precipitation.

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2405 Equine Senior Wellness II

CBCFibrinogen

Specimen Requirements Chemistry/Insulin: 2.0 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.5 mL LTT and air dried peripheral blood smearsACTH Endo: 1.0 mL Frozen EDTA Plasma, CTT. Samples for Insulin testing CAN NOT be hemolyzed.

Reported Time See comment

InsulinACTH Endogenous

Total T4Equine Chem Screen

Collection Notes The LTT for ACTH Endo MUST be spun down within 30 minutes of collection and the plasma separated into a non-additive tube such as a CTT. Freeze until transported to lab. Insulin samples MUST be spun down within 30 minutes; if submission is to be delayed, draw a second tube or, volume allowing, pull some serum off the SST/RTT and freeze for insulin testing. Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 30 minutes of collection; this then does not require collection of a GTT; this also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: SDH is included in Equine Chemistry Screen.Equine insulin and glucose levels have been suggested for diagnosing insulin dysregulation, but can be abnormal in any disease causing insulin resistance. For more information, please visit our website to view our test service update on Equine insulin testing.Testing not recommended on samples >24 hours old due to rapid deterioration.

Chemistries and CBC reported out day. ACTH, Endo and Insulin run Monday/Wednesday/Friday, reported out same day.

Fibrinogen performed by heat precipitation.

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2872 Equine Special w/ Insulin

CBCFibrinogen

Specimen Requirements Chemistry/Insulin: 1.0 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.5 mL LTT and air dried peripheral blood smears.

Reported Time See Comment

Total T4Equine Chem Screen

Insulin

Collection Notes Insulin samples MUST be spun down within 30 minutes; if submission is to be delayed, draw a second tube or, volume allowing, pull some serum off the SST/RTT and freeze for insulin testing. Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 30 minutes of collection; this then does not require collection of a GTT; this also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: SDH is included in Equine Chemistry Screen.Equine insulin and glucose levels have been suggested for diagnosing insulin dysregulation, but can be abnormal in any disease causing insulin resistance. For more information, please visit our website to view our test service update on Equine insulin testing.Testing not recommended on samples >24 hours old due to rapid deterioration.

Chemistry and CBC results out same day.Insulin run Monday/Wednesday/Friday with results out same day.

Fibrinogen performed by heat precipitation.

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859 Feline Panel

CBCChemistry Screen

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time See Comments

CK (Creatine Kinase)Total T4

FeLV (ELISA)FIV (IFA)FCoV (FIP) Screen (IFA)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistry, CBC and FeLV run same day. FIV/FIP run daily, AM. FIV Recommend repeat in 6-8 weeks, if result is weak positive. Vaccinated cats will have a positive antibody response indistinguishable from that of infected cats for at least one year post vaccination.

2247 Free T4

Methodology: Chemiluminescent Immunoassa

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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1247 Free T4,  Add-On

Methodology: Chemiluminescent Immunoassa

Specimen Requirements 0.5 mL Serum SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Available as an add-on to an existing panel (within 7 days). Previous case ID is required to activate the discounted price.

77 General Diagnostic w/ T4

CBC

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Chemistry Screen Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

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8571 General Diagnostic w/ T4 and Feline Viral I

CBCChemistry Screen

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time See Comment

Total T4FeLV (ELISA)

FIV (IFA)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistry, CBC and FeLV run same day. FIV run daily, AM.Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

855 General Diagnostic w/ T4 and Feline Viral II

CBCChemistry Screen

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time See Comments

Total T4FeLV (ELISA)

FCoV (FIP) Screen (IFA)FIV (IFA)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistry, CBC, and FeLV run same day, FIV/FIP run daily, AM.

Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted. Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

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2077 General Diagnostic w/ T4 and FeLV

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Total T4 FeLV

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

2072 General Diagnostic w/ Thyroid II

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Total T4 cTSH (Thyroid Stimulating Hormone)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: TSH only validated on canines, but has been used in felines.

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777 General Diagnostic w/T4 and Cobalamin/Folate

CBCChemistry Screen

Specimen Requirements Chemistry: 1.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears. Sample MUST be non-hemolyzed for folate testing and avoid lipemic samples.

Reported Time See additional comments Below

CobalaminFolate

Total T4

Collection Notes Patient must be fasted 12-18 hours (diabetic animals 6-8 hours). Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Cover samples with foil or a paper towel to protected from light (folate and cobalamin are light sensitive).Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistries and CBC run same day. Cobalamin and Folate are run in-house Monday, Wednesday, and Friday; reported out same day.Folates >24.0 ug/dL and cobalamin >1000 are re-run on dilution and reported.

7711 General Diagnostic w/T4, Follow-Up

CBC

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Chemistry Screen Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

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61 General Endocrine, Canine

Chemistry Screen

Specimen Requirements 2 RTTs, Pre/Post 0.5 mL Serum Each, 1.0 mL GTTDO NOT USE SST TO SPIN DOWN SAMPLE

Reported Time Same Day

Total T4 ACTH Response (Pre and Post Cortisols)

Collection Notes A grey top tube is recommended but not necessary if the RTT is centrifuged and serum is transferred to a transport tube. PROTOCOL:1. Collect a resting sample in a RTT, 0.5 mL of serum is required. Separate serum if possible and refrigerate. 2. Inject 0.25 mg (250 µg) of Cortrosyn IM or IV (1 vial). 3. Collect a second sample EXACTLY 1 HOUR post injection in RTT, label clearly as to the collection status (post), and preserve in the same manner as the first sample. NOTE: The test has been validated using Cortrosyn. Although compounded gels are available, they vary greatly in peak times and are not recommended.

ALTERNATE CANINE PROTOCOL (Microdose): In order to keep Cortrosyn costs down, use the specific dose of 5 ug/kg IV - up to the maximum dose of 250 ug (one vial) per dog. Whatever is left over can then be split into aliquots of 25 ug or 50 ug using small plastic syringes. Normally 1 mL of sterile physiologic saline is used as diluent. If so, 25 ug would be 100 uL or 0.1 mL. ***When the patient is receiving Trilostane/Vetoryl, the timing of the test is important. The pre-cortisol sample should be drawn 3-5 hours after the morning capsule. The test is a one hour test, so in other words, the ACTH Response test should be completed 4-6 hours after the medication, which is usually given once a day in the morning.

Additional Comments: Pre and Post sample required. In addition to diagnosis of naturally occurring hyperadrenocorticism (Cushing's disease), this is the only diagnostic test for hypoadrenocorticism (Addison's disease), iatrogenic hyperadrenocorticism, and monitoring response to medical therapy of Cushing's disease. Prednisone, prednisolone, and other corticosteroids (except dexamethasone) will cross react. Recommend these drugs be discontinued at least 48 hrs before testing. If dog has been on prolonged steroids, patient must be tapered with the drug stopped for at least 3 weeks to let the adrenal gland recover. INTERPRETATION HYPERADRENOCORTICISM: An ACTH response test is done to CONFIRM clinical suspicion of hyperadrenocorticism. Stressful diseases (ill diabetics, renal failure, etc.) and current drug therapy may cause false positives. If the cortisol results are normal (and an adequate dose of Cortrosyn was administered) and there is high clinical suspicion of hyperadrenocorticism, sex hormone hyperadrenocorticism (atypical Cushing's) should be considered. Additional testing on the serum from this ACTH stimulation test can be performed. Please contact us within 7 days of this cortisol result for further testing options. A low-dose dexamethasone suppression test should also be considered, as some patients with Cushing's will be positive on one test but not on the other.INTERPRETATION HYPOADRENOCORTICISM: Pre and post cortisol <2 ug/dL are supportive of hypoadrenocorticism but need to be interpreted in light of other clinical findings and history. Failure to stimulate can occur with iatrogenic hyperadrenocorticism, dogs being treated for hyperadrenocorticism and Addison's disease.

For Feline Protocol See ACTH Response, Feline 1039

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634 Insulin

Methodology: Chemiluminescent Immunoassa

Specimen Requirements 1.0 mL Serum, Minimum: 0.5 mL, SST/RTT, Frozen**Serum can NOT be hemolyzed as hemolysis will interfere with testing.**

Reported Time Mon, Wed, Fri, Same day

Collection Notes Centrifuge tube within 20-30 minutes of collection. Separate serum into a transport tube. Sample should be tested or frozen within 24 hours. If unable to centrifuge, use GTT for collection of glucose samples. GTT tubes must have a minimum of 1 mL of blood for accurate results.

Additional Comments: In combination with clinical signs and concurrent blood glucose concentration, determination of serum insulin may be useful in diagnosing an insulinoma. In assessing whether the serum insulin concentration is appropriate, it must be compared to the serum glucose concentration (taken concurrently; included in the cost of insulin test). Measurement of serum insulin concentrations is most useful when the blood glucose concentration is 59 mg/dL or less.Equine insulin and glucose levels have been suggested for diagnosing pituitary adenoma/Cushing's disease, but can be abnormal in any disease causing insulin resistance. Testing not recommended on samples >24 hours old due to rapid deterioration.

2217 Lipid Panel w/ T4

Methodology: Spectrophotometry

Cholesterol

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

Triglycerides Total T4

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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620 Luteinizing Hormone

Methodology: RIM

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Please allow sample to clot (up to 20 minutes) and then spin down sample. If using a RTT, remove serum and put into a fresh RTT/CTT. Avoid lipemia and hemolysis.

Additional Comments: OVARIAN REMNANT DETERMINATION: Test when the dog is NOT showing signs of estrus: A positive result means high LH concentrations, indicating no ovarian tissue. (Occasionally false positives may occur with the 24 hr LH surge.) A negative result means low LH concentrations, indicating the presence of ovarian tissue. Exposure to estrogen creams will also cause a negative LH result.OVULATION TIMING IN THE BITCH: The duration of the LH peak varies from dog to dog and lasts only 24 hours in approximately 40% of the bitches. For this reason, serum should be tested for LH every day beginning on the 4th or 5th day of proestrus or when vaginal cytology approaches 50% cornification. When a positive result is obtained this is considered "Day 0". A progesterone test should be done on Day 0 and Day 3 to confirm a rise in progesterone. If the detected increase in LH was the actual preovulatory surge, progesterone concentrations should increase above 2 ng/mL within 3 days and stay elevated. If the progesterone remains low, less than 2 ng/mL, it indicates that a proestrus fluctuation in LH was identified. Continue daily testing until the true LH surge occurs. Days 4 through 7 post LH surge encompass the true fertile period with peak fertility on Days 5 and 6. Breeding on Days 2, 4, and 6 post LH surge will completely cover the fertile period and maximize viable sperm numbers on the bitch's most fertile days. Indeterminant results may indicate a negative result or that the LH surge is approaching or recently peaked. For animals with unknown ovarian status, this result may indicate that the animal has been ovariectomized. NOTE: This test has not been validated for, but has been used in, cats with similar interpretations as in dogs.

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547 Progesterone, Canine

Methodology: Chemiluminescent Immunoassa

Specimen Requirements 0.5 mL Serum, RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.Remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Canine: To best predict optimal breeding dates, serial progesterone results should be interpreted in conjunction with Vaginal Cytology (Canine Breeding Panel, Test 1647). At ovulation, serum progesterone concentration ranges from 3.8-10 ng/mL. Optimal breeding (fresh semen) is 2 days after ovulation (if frozen semen: 4 days after ovulation). For timing of whelping, progesterone decreases to <2 ng/mL 36-48 hours prior to whelping. After the initial test, follow-up testing is offered; order Progesterone Follow-up, Canine 747.

This test code is for canine use only. All other species use Progesterone test code #647

747 Progesterone, Follow-Up

Methodology: Chemiluminescent Immunoassa

Specimen Requirements 0.5 mL Serum, RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.Remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This is to be used for canine prebreeding or prewhelping if multiple testing is needed.

Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

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647 Progesterone, Non-Canine

Methodology: Chemiluminescent Immunoassa

Specimen Requirements 0.5 mL Serum, RTTMinimum: 0.3 mL Serum

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.Remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Mare: Corpus luteal values are 7-8 ng/mL by 6 days after ovulation. There is a transient decrease at 28 days of pregnancy to around 5 ng/mL and then concentration increases to day 50 and plateaus at day 140 (15-20 ng/mL). Values then decline so that at 180-200 days levels are below 1 ng/mL. Cow: Levels of 6 ng/mL are required to maintain pregnancy. Camelids: Values of about 2.0 ng/mL indicate luteal activity.

For canines, please order Canine Progesterone 547

300 Relaxin, Canine or Feline

Methodology: RIM

Specimen Requirements 0.5 mL Serum, SST/RTT.Test can also be run on heparinized or EDTA Plasma (GrnTT/LTT).Markedly hemolyzed or lipemic samples should not be used.

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Day 20 of gestation is found to be the earliest day of pregnancy detection. Relaxin rises after implantation of a fertilized egg. Implantation occurs 26-31 days after the LH surge. Any negative result is considered indicative of non-pregnancy; however, two negative results one week apart may be required for confirmation of this status, especially when date of mating is unknown. A positive result is considered indicative of pregnancy. A weak positive result may be observed at the beginning of the pregnancy. It is recommended to repeat the test on a fresh sample one week later to confirm pregnancy.

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2231 Resting Cortisol

Methodology: Chemiluminescent Immunoassa

Specimen Requirements 0.5 mL Serum, RTT

Reported Time Same Day

Collection Notes If the patient has been on steroids for >14 days, patient should be taperdown and discontinue for at least 2 weeks prior to testing. If <14 days, discontinue for 38 hours prior to testing.DO NOT USE SST TO COLLECT OR SPIN DOWN SAMPLE

Additional Comments: A patient with a resting cortisol >2.0 ug/dL has a 99% chance of NOT having Addison's disease.With a resting cortisol of <2.0 ug/dL, an ACTH Response 39 is needed to rule-in Addison's disease. This interpretation is valid if no oral or topical steroids have been given in the last 6 weeks. A resting cortisol cannot be used to rule- in or out hyperadrenocorticism. A LDDS 38 and/or ACTH Response 39 needs to be performed.

4001 RX - Methimazole I

CBCUrine Specific GravityTotal T4

Specimen Requirements Chemistry: 0.5 mL serum, SST/RTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrine SG 1.0 mL urine

Reported Time Same Day

BUN (Blood Urea Nitrogen)Creatinine

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Run at 1 month, then every 4 to 6 months. Generic: Methimazole, Brand: Tapazole, Felimazole

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4007 RX - Methimazole II

CBCTotal T4

Specimen Requirements Chemistry: 0.5 mL serum, SST/RTTCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

ALP (Alkaline Phosphatase) ALT (Alanine Transaminase)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Run at 1 month, then every 4 to 6months.Generic: Methimazole, Brand: Tapazole, Felimazole

5021 RX - Thyroxine/Soloxine

Methodology: Spectrophotometry

CBC

Specimen Requirements 0.3 mL Serum, SST/RTT

Reported Time Same Day

Total T4 Cholesterol

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Run after 1 month, then every 3 to 6 months.(Levothyroxine, Soloxine)Same generic brand as methimazole

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5011 RX- Cushing's (Trilostane, Mitotane)

CBCUrinalysisACTH Response

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTTCBC: 1.0 mL LTTACTH Response: 0.5 mL Serum each, Pre and Post, from RTT ONLY

Reported Time Same Day

CreatinineSodiumPotassium

BUN (Blood Urea Nitrogen)ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)

Collection Notes DO NOT USE SST TO COLLECT OR SPIN DOWN SAMPLE for ACTH Response Test.

Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.Remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Please see ACTH Response 39 for collection protocol.

Additional Comments: Run prior to and immediately after induction, then every 3 to 4 months, more if patient's condition dictates.

Please see ACTH Response 39 for additional information.

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1716 Senior Routine Screen

CBCGlucoseBUN (Blood Urea Nitrogen)CreatinineBUN/Creat RatioSodiumPotassium

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urine

Reported Time Same Day

Sodium/Potassium RatioChlorideCalciumPhosphorusTotal ProteinAlbuminGlobulin

Albumin/Globulin ratioBilirubin TotalALP (Alkaline Phosphatase)ALT (Alanine Transaminase)Total T4Urinalysis

Collection Notes Please indicate method of urine collection. Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: None

11 Senior Screen

Methodology: Spectrophotometry

GlucosePotassiumPhosphorusChloride

Specimen Requirements 0.5 mL Serum SST/RTT

Reported Time Same Day

BUN (Blood Urea Nitrogen)CreatinineNa/K RatioTotal T4

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)Sodium

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

For a Senior Screen w/ CBC, order 899

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4705 Senior Screen w/ Calcium and HCT

GlucosePotassiumSodiumPhosphorusHCT

Specimen Requirements 0.5 mL of each, Serum, SST/RTT, 1.0 mL GTT, and 0.1 mL Whole Blood, LTT. A sealed crit w/ blood also accepted in lieu of LTT.

Reported Time Same Day

CreatinineChlorideTotal T4Calcium

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)BUN (Blood Urea Nitrogen)Na/K Ratio

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

3047 Senior Screen W/ CBC and Free T4

CBCGlucosePotassiumSodiumPhosphorus

Specimen Requirements Chemistry: 0.75 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

CreatinineChlorideTotal T4Free T4

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)BUN (Blood Urea Nitrogen)Na/K Ratio

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

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1866 Senior Screen w/ Free T4

GlucosePotassiumSodiumNa/K RatioPhosphorus

Specimen Requirements 0.5 mL Serum SST/RTT

Reported Time Same Day

BUN (Blood Urea Nitrogen)CreatinineTotal T4Chloride

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)Free T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

2071 Senior Screen W/ TSH

GlucosePotassiumSodiumPhosphorus

Specimen Requirements 0.5 mL Serum, SST/RTT, 1 mL GTT

Reported Time Same Day

BUN (Blood Urea Nitrogen)CreatinineChlorideTotal T4

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)Na/K RatioTSH (Thyroid Stimulating Hormone)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: TSH only validated on canines, but has been used in felines. Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

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4701 Senior Screen w/Calcium

Methodology: Spectrophotometry

GlucosePotassiumSodiumPhosphorus

Specimen Requirements 0.5 mL Serum, SST/RTT, 1.0 mL GTT

Reported Time Same Day

CreatinineChlorideTotal T4Calcium

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)BUN (Blood Urea Nitrogen)Na/K Ratio

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

1101 Senior Screen w/Cholesterol

Methodology: Spectrophotometry

GlucosePotassiumSodiumNa/K Ratio

Specimen Requirements 0.5 mL Serum SST/RTT, 1.0 mL GTT

Reported Time Same Day

BUN (Blood Urea Nitrogen)CreatinineTotal T4Chloride

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)CholesterolPhosphorus

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

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872 Special Equine Profile

CBCEquine Chemistry Screen

Specimen Requirements Chemistry: 1.0 mL Serum,SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Total T4 Fibrinogen

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: SDH is included in Equine Chemistry Screen.

651 T3 (Triiodothyronine)

Methodology: Chemiluminescent Immunoassa

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Mondays, Same day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Test is run every Monday.

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9 T3 Suppression I

Methodology: Spectrophotometry

Pre Total T4

Specimen Requirements 2 SST/RTT, Pre T4/Post T4, 0.3 mL Serum each

Reported Time Same Day

Post Total T4

Collection Notes Protocol: 1.  Day 1:  Collect baseline (pre) T4 sample.  Label tube "Pre" 2.  Day 1 & 2:  Administer 25 ug PO of T3 supplement (Cytobin or Cytomel) every 8 hours for 7 doses. 3.  Day 3:  Administer the last (7th) dose of T3 supplement on the morning of the third day and 2-4 hours later draw sample for a second T4 determination. Label tube "Post".Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: T3 suppression is done to CONFIRM feline hyperthyroidism. Interpretation of Post-suppression T4 concentration: Normal cats:  < 1.5 ug/dL or suppression greater than 50% of pre-T4 value.Inconclusive:  1.5 - 2.0 ug/dL Hyperthyroid:  > 2.0 ug/dL. If the post T4 sample is not adequately suppressed, it may be due to: 1) the cat did not receive an adequate amount of T3, or 2) the cat is hyperthyroid. Measurement of T3 concentration in the pre and post suppression samples (Test 1009 - T3 Suppression II) can be useful in verifying that the patient received the hormone supplementation.

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1009 T3 Suppression II

Methodology: Chemiluminescent Immunoassa

Pre and Post T4

Specimen Requirements Pre and Post required, 0.5 mL (minimum 0.3mL) serum each.

Reported Time Mondays, Same Day

Pre and Post T3

Collection Notes Protocol: 1. Day 1 - collect baseline (pre) T3 and T4. Label tube "Pre" 2. Day 1&2 - administer 25ug PO of T3 supplement Cytobin or Cytomel) every 8 hours for 7 doses.3. Day 3 - administer the last (7th) dose of T3 supplement on the morning of the third day and 2-4 hours later collect sample for a second T3 and T4 determination. Label tube "Post".Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: T3 suppression is done to CONFIRM feline hyperthyroidism. Submit both samples at one time to allow analysis on the same instrument run. Interpretation of Post-suppression T4 concentration: Normal cats:  < 1.5 ug/dL or suppression greater than 50% of pre-T4 value.Inconclusive:  1.5 - 2.0 ug/dL Hyperthyroid:  > 2.0 ug/dL. If the post T4 sample is not adequately suppressed, it may be due to: 1. The cat did not receive an adequate amount of T3 2. the cat is hyperthyroid.

74 Thyroid I

Methodology: Chemiluminescent Immunoassa

Free T4

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Next day

TSH (Thyroid Stimulating Hormone)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: TSH only validated on canines, but has been used in felines.

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75 Thyroid II

Total T4

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

TSH (Thyroid Stimulating Hormone)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: TSH only validated on canines, but has been used in felines. Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

864 Thyroid III

TSH (Thyroid Stimulating Hormone)

Specimen Requirements 1.0 mL Serum, SST/RTT

Reported Time Same Day

Total T4 Free T4

Collection Notes Peak samples are drawn 4-6 hours post pill Trough samples are drawn just before the next dose.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: TSH only validated on canines, but has been used in felines.Feline T4: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

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874 Thyroid IV

Total T4

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

Free T4

Collection Notes Peak samples are drawn 4-6 hours post pill. Trough samples are drawn just before the next dose.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

884 Thyroid Panel V

Free T4

Specimen Requirements 1.0 mL Serum, SST/RTT

Reported Time See Comment

Total T4 T3

Collection Notes Peak samples are drawn 4-6 hours post pill. Trough samples are drawn just before the next dose.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Free and Total T4 run dailyT3 run every Monday, with results out same day. Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

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49 Thyroid Screen

Total T4

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time See Comment

T3 (Triiodothyronine)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: T4 run same day, T3 run every Monday. Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

244 Total T4

Methodology: Spectrophotometry

Specimen Requirements 0.5 mL Serum, SST/RTT. Minimum: 0.2 mL Serum.

Reported Time Same Day

Collection Notes For dogs, peak samples are drawn 4-6 hours post pill Trough samples are drawn just before the next dose.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

1237 Total T4, Add-On

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Peak samples are drawn 4-6 hours post pill Trough samples are drawn just before the next dose.Please allow sample to clot (up to 20 minutes) and then spin down sample. If using a RTT, remove serum and put into a fresh RTT/CTT. Avoid lipemia and hemolysis.

Additional Comments: Felines only: In the geriatric cat with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted. Available as an add-on to an existing panel (within 7 days). Previous case ID is required to activate the discounted price.

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6971 TRH Stimulation Test

Methodology: Chemiluminescent Immunoassa

ACTH, Endo Pre

Specimen Requirements 0.5 mL EDTA Plasma for Pre and Post samples, Separated and Frozen, CTT

Reported Time Mon/Wed/Fri, Same day

ACTH, Endo Post

Collection Notes Procedure:HORSES CAN BE FASTED OR MAY HAVE BEEN RECENTLY FED HAY, BUT NOT GRAIN. DO NOT PERFORM IMMEDIATELY AFTER AN ORAL SUGAR TEST.1. Collect 0 hour (PRE) TRH sample in LTT. Spin and separate plasma into a plastic transport tube within 1 hour of collection. Recommended to freeze immediately. Clearly label as 0 hour / PRE-sample.2.Inject 1 mg TRH (Tyrotropin Releasing Hormone) intravenously (IV) for horses >250 kg; inject 0.5 mg TRH IV for horses and ponies <250 kg. TRH can be obtained from compounding pharmacies.The online pharmacy Wedgewood sells TRH under the brand name Protirelin.3. At exactly 10 minutes after administration of TRH, collect second sample in LTT. Spin and separate plasma into a plastic transport tube within 1 hour of collection. Recommended to freeze immediately. Clearly label tube 10 minutes /POST-sample.

Additional Comments: Endogenous ACTH is tested on both samples. Recommended to perform test mid-November to mid-July since seasonally adjusted reference intervals have not yet been established. This is currently the recommended test for early detection of equine Pituitary Pars Intermedia Dysfunction (PPID) if a horse has clinical signs, and endogenous ACTH is inconclusive/negative.Transient yawning, coughing, mouth chomping and display flehmen response can follow administration of TRH. These side effects will subside within a few minutes.

248 TSH (Thyroid Stimulation Hormone)

Methodology: Chemiluminescent Immunoassa

Specimen Requirements 0.5 mL Serum, SST/RTT. Minimum: 0.2 mL Serum.

Reported Time Same Day

Collection Notes None

Additional Comments: Run on canines (for confirmation of hypothyroidism) and felines (for confirmation of hyperthyroidism.)

TSH only validated on canines, but has been used in felines.

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2481 TSH, Add-On

Methodology: Chemiluminescent Immunoassa

Specimen Requirements 0.5 mL Serum, SST/RTT. Minimum: 0.2 mL Serum.

Reported Time Same Day

Collection Notes None

Additional Comments: Run on canines (for confirmation of hypothyroidism) and felines (for confirmation of hyperthyroidism.)TSH only validated on canines, but has been used in felines.

Available as an add-on to an existing panel (within 7 days). Previous case ID is required to activate the discounted price.

234 Urine Cortisol/Creatinine Ratio

Urine Cortisol

Specimen Requirements 0.5 mL Urine, Urine Tube/CTTIf sample is bloody or very cloudy/cellular, submit a larger volume (1.0 mL minimum) as the sample will need to be centrifuged.

Reported Time Same Day

Urine Creatinine Ucor/Ucreat Ratio

Collection Notes If the patient has been on steroids for >14 days, discontinue for 2 weeks prior to testing. If <14 days, discontinue for 48 hours prior to testing.

Additional Comments: INTERPRETATION:An elevated urine cortisol/creatinine ratio (> 20) provides additional support for canine hyperadrenocorticism but is not specific for that condition. Many disorders cause an increased result. A normal result (<20) makes hyperadrenocorticism very unlikely. Further testing (low dose Dexamethasone Suppression Test and/or ACTH Stimulation Test) is advised to establish a diagnosis of hyperadrenocorticism.

*Note that both cortisol and creatinine have been converted to moles to get this ratio.

233 Urine, Cortisol

Methodology: Chemiluminescent Immunoassa

Specimen Requirements 0.5 mL Urine, Urine Tube/CTTIf sample is bloody or very cloudy/cellular, submit a larger volume (0.5 mL minimum) as the sample will need to be centrifuged.

Reported Time Same Day

Collection Notes None

Additional Comments: None

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1711 Wellness Screen II

CBCChemistry ScreenUrinalysis

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urine

Reported Time Same Day

AmylaseLipase

CK (Creatine Kinase)Total T4

Collection Notes Please indicate method of urine collection. Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: To request this panel, but submit the urinalysis portion later, see Wellness Screen II w/ Urine to Follow 1711NU

1721 Wellness Screen II w/ SDMA

CBCChemistry Screen Urinalysis

Specimen Requirements Chemistry: 1.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urine

Reported Time See comment

AmylaseLipaseSDMA

CK (Creatine Kinase)Total T4

Collection Notes Please indicate method of collection.Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: CBC, Chemistries, and urinalysis run same day, SDMA Results Next Day. See SDMA SO513 for more details about test.

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1711NU Wellness Screen II w/ Urine to Follow

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

AmylaseLipase

CK (Creatine Kinase)Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: When submitting the urine, please request Urine, No Charge 179 on a separate test request form, and include the previous case ID.

1712 Wellness Screen III

CBCChemistry ScreenFree T4

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same day

AmylaseLipase

CK (Creatine Kinase)Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Panel includes Wellness Screen I and Free T4. Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

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1713 Wellness Screen IV

CBCChemistry ScreenUrinalysis

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urine

Reported Time Same day

AmylaseLipaseFree T4

CK (Creatine Kinase)Total T4

Collection Notes Please indicate method of urine collection. Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

15 Wellness/Geriatric Panel

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

AmylaseLipase

CK (Creatine Kinase)Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: None

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1722 Wellness/Geriatric Panel w/ SDMA

CBCChemistry ScreenSDMA

Specimen Requirements Chemistry: 1.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time See comment

AmylaseLipase

CK (Creatine Kinase)Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: CBC and Chemistries run same day, SDMA results next day. See SDMA SO513 for more details about test.

1160 Wellness/Geriatric Panel w/Triglycerides

CBCChemistry ScreenTriglycerides

Specimen Requirements Chemistry: 0.5 mL serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT, air dried peripheral blood smears

Reported Time Same Day

AmylaseLipase

CK (Creatine Kinase)Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: None

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1015 Wellness/Geriatric Panel, Follow-Up

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT, air dried peripheral blood smears

Reported Time Same Day

AmylaseLipase

CK (Creatine Kinase)Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

881 Wellness/Geriatric Profile w/ Feline Viral I

CBCChemistry ScreenAmylase

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time See Comment

LipaseCK (Creatine Kinase)Total T4

FeLV (ELISA)FIV (IFA)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistry, CBC, and FeLV run same day, FIV run daily, AM. Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted. Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

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858 Wellness/Geriatric Profile w/ Feline Viral II

CBCChemistry ScreenAmylase

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time See Comments

LipaseCK (Creatine Kinase)Total T4

FeLV (ELISA)FIV (IFA)FCoV (FIP) Screen (IFA)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Chemistry, CBC, and FeLV run same day, FIV/FIP run daily, AM. Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted. Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

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Histopathology Test Selection and Submission Guidelines

When submitting histopathology specimens, please provide a detailed history – including previous lab accession numbers, clinical signs, and treatment. Label all samples with patient name, sample site, and submission fluid (formalin or saline). On the requisition form, be sure to indicate the marking system used for tissue orientation or site identification. Sutures are recommended if orientation of the tissue is required (1 suture = cranial). Sutures or individually labeled cassettes/bottles are recommended for site identification. If culture or other testing requiring fresh tissue or special handling is needed on a shared histopathology submission, please alert the laboratory on the requisition form and provide any necessary instruction.

Packaging and Transportation Small & Medium Histopathology Submissions: A 10:1 formalin to tissue ration is recommended; however, when shipping by air or long distance, use minimal formalin. Submit tissues intact for margin examination, specifically avoiding incision of the deep margin. Securely seal containers and include absorbent material and requisition form in outermost bag. Large Histopathology Submissions: When transporting large tissues, such as whole spleens, use saline to moisten only – not formalin. Place the sample in a small amount of saline, wrapping gauze around the tissue to keep moist. Please do not send tissues in large amounts of liquid. Securely triple bag and include absorbent material and request form in outermost bag.

Reporting Options While both the Mini report and Full report provide a gross description, microscopic diagnosis and comments, a Full report additionally provides a microscopic description. Please note, reporting time is dependent on tissue type and size; larger samples require longer fixation and processing, while samples with bone or mineralization may require decalcification prior to processing. We also offer pathologist interpretation on previously stained H&E slides at a reduced rate. These test selections are identified in name with “SLIDES ONLY.” Please call the laboratory for additional information.

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Selecting the Correct Test See the explanations below to help select the correct test for the sample provided:

Single Tissue: 1-4 samples from one site (one surgical site or one organ type), where L+W+H is less than 15 cm in aggregate Additional Single Tissues Orders: Each additional site or each additional 4 samples from the same site, where L+W+H is less than 15 cm in aggregate

Complex Tissue:

• 1-4 samples from one site (one surgical site or one organ type), where L+W+H is greater than 15 cm in aggregate

• Each entire organ (examples: brain, heart, lung, spleen, liver, gallbladder, pancreas, 1 kidney, 1 eyeball, 1 ear canal, 1 mammary chain, 1-2 ovaries or testicles)

• Each sample requiring decalcification (examples: 1 digit, tail, ribs, mandible) Note: Additional Single Tissue or Additional Skin Punch Biopsies orders may be combined with Complex Tissue orders.

Skin Punch Biopsies: 1-4 skin punches (1-8 mm) Additional Skin Punch Biopsies: each additional 4 skin punches (1-8 mm)

Limb Dissection: Includes dissection, representative section submission, decalcification, and margin examination. Radiographs and history are appreciated. Refrigerate immediately, or store on ice. Submit limb fresh, in multiple securely sealed plastic bags, with few ice pack/s. Please do not freeze or fix.

Necropsy: We offer a Gross Only Necropsy option, when the cause of death is obvious upon gross inspection, or a Full Necropsy option, which includes histopathology and ancillary in-house testing as requested by the pathologist. Please call the laboratory for pricing, transportation, and aftercare of remains. Refrigerate body, and submit for necropsy as soon as possible. If refrigeration is not available, pack body with ice packs. Prevent freezing.

Special Stains A variety of special stains can be performed at your request for an additional fee. This price includes staining and interpretation. The paraffin block is preferred; however, unstained tissue slides may also be used.

Immunohistochemistry Upon your request, in-house and reference laboratory IHC can be performed at an additional fee. This price includes staining and interpretation. The paraffin block is preferred; however unstained tissue slides (charged slides) may also be used. Please call the laboratory for specific test options, requirements and pricing.

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Histopathology

1400 Histopathology Single Tissue - Mini Path Review, SLIDES ONLY

Specimen Requirements H&E stained tissue slide - Single Tissue (1-4 samples from one surgical site or one organ type, where length + width + height is less than 15cm in aggregate)

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Mini report includes diagnosis and comments.

This test is for received H&E slides ONLY; if submitting tissue sample in formalin, please see test code 400.

1401 Histopathology Single Tissue - Full Path Review, SLIDES ONLY

Specimen Requirements H&E stained tissue slides - Single Tissue (1-4 samples from one surgical site or one organ type, where length + width + height is less than 15cm in aggregate)

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Full report includes microscopic description, diagnosis, and comments.

This test is for received H&E slides ONLY; if submitting tissue sample in formalin, please see test code 401.

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1403 Histopathology Complex Tissue - Full Path Review, SLIDES ONLY

Specimen Requirements H&E stained tissue slide - Complex Tissue (Each 1-4 samples from one surgical site or one organ type, where length + width + height is greater than 15cm in aggregate. Each entire organ.)

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Full report includes microscopic description, diagnosis, and comments.

This test is for received H&E slides ONLY; if submitting tissue sample in formalin, please see test code 403.

1418 Histopathology Additional Skin Punches - Full, SLIDES ONLY

Specimen Requirements H&E stained tissue slides - Additional 'Skin Punch Biopsies' (Additional 1-4 skin punch biopsies)

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Full report includes microscopic description, diagnosis, and comments.

This test is for received H&E slides ONLY; if submitting tissue sample in formalin, please see test code 418.

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1419 Histopathology Add'l SingleTissue - Full, SLIDES ONLY

Specimen Requirements H&E stained tissue slides - Additional 'Single Tissue' (Additional 1-4 samples from one surgical site or organ type, where length + width + height is less than 15cm in aggregate)

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Full report includes microscopic description, diagnosis, and comments.

This test is for received H&E slides ONLY; if submitting tissue sample in formalin, please see test code 419.

1419X2 Histopathology Add'l Single Tissue x2 - Full, SLIDES ONLY

Specimen Requirements H&E stained tissue slides - Two 'Additional Single Tissues' (2 x Additional 1-4 samples from one surgical site or organ type, where length + width + height is less than 15cm in aggregate)

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Full report includes microscopic description, diagnosis, and comments.

This test is for received H&E slides ONLY; if submitting tissue sample in formalin, please see test code 419x2.

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1419X3 Histopathology Add'l Single Tissue x3 - Full, SLIDES ONLY

Specimen Requirements H&E stained tissue slides - Three 'Additional Single Tissues' (3 x Additional 1-4 samples from one surgical site or organ type, where length + width + height is less than 15cm in aggregate)

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Full report includes microscopic description, diagnosis, and comments.

This test is for received H&E slides ONLY; if submitting tissue samples in formalin, please see test code 419x3.

1419X4 Histopathology Add'l Single Tissue x4 - Full, SLIDES ONLY

Specimen Requirements H&E stained tissue slides - Four 'Additional Single Tissues' (4 x Additional 1-4 samples from one surgical site or organ type, where length + width + height is less than 15cm in aggregate)

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Full report includes microscopic description, diagnosis, and comments.

This test is for received H&E slides ONLY; if submitting tissue samples in formalin, please see test code 419x4.

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1423 Histopathology Skin Punch Biopsies - Mini, SLIDES ONLY

Specimen Requirements H&E stained tissue slides: 1-4 Skin punch biopsies

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Mini report includes diagnosis and comments.

This test is for received H&E slides ONLY; if submitting tissue samples in formalin, please see test code 423

1425 Histopathology Skin Punch Biopsies - Full, SLIDES ONLY

Specimen Requirements H&E stained tissue slides: 1-4 Skin punch biopsies

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Full report includes microscopic description, diagnosis, and comments.

This test is for received H&E slides ONLY; if submitting tissue samples in formalin, please see test code 425.

1440 Histopathology Additional Skin Punches - Mini, SLIDES ONLY

Specimen Requirements H&E stained tissue slides - Additional 'Skin Punch Biopsies' (Additional 1-4 skin punch biopsies)

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Mini report includes diagnosis and comments.

This test is for received H&E slides ONLY; if submitting tissue samples in formalin, please see test code 440.

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1441 Histopathology Add'l Single Tissue - Mini, SLIDES ONLY

Specimen Requirements H&E stained tissue slides - Additional 'Single Tissue' (1-4 samples from one surgical site or one organ type, where length + width + height is less than 15cm in aggregate)

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Mini report includes diagnosis and comments.

This test is for received H&E slides ONLY; if submitting tissue samples in formalin, please see test code 441.

1441X2 Histopathology Add'l Single Tissue x2 - Mini, SLIDES ONLY

Specimen Requirements H&E stained tissue slides - Two 'Additional Single Tissues' (2 x Additional 1-4 samples from one surgical site or organ type, where length + width + height is less than 15cm in aggregate)

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Mini report includes diagnosis and comments.

This test is for received H&E slides ONLY; if submitting tissue samples in formalin, please see test code 441x2.

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1441X3 Histopathology Add'l Single Tissue x3 - Mini, SLIDES ONLY

Specimen Requirements H&E stained tissue slides - Three 'Additional Single Tissues' (3 x Additional 1-4 samples from one surgical site or organ type, where length + width + height is less than 15cm in aggregate)

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Mini report includes diagnosis and comments.

This test is for received H&E slides ONLY; if submitting tissue samples in formalin, please see test code 441x3.

1441X4 Histopathology Add'l Single Tissue x4 - Mini, SLIDES ONLY

Specimen Requirements H&E stained tissue slides - Four 'Additional Single Tissues' (4 x Additional 1-4 samples from one surgical site or organ type, where length + width + height is less than 15cm in aggregate)

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Mini report includes diagnosis and comments.

This test is for received H&E slides ONLY; if submitting tissue samples in formalin, please see test code 441x4.

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1463 Histopathology Complex Tissue - Mini Path Review, SLIDES ONLY

Specimen Requirements H&E stained tissue slide - Complex Tissue ( 1-4 samples from one surgical site or one organ type, where length + width + height is greater than 15cm in aggregate. Each entire organ.)

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Mini report includes diagnosis and comments.

This test is for received H&E slides ONLY; if submitting tissue samples in formalin, please see test code 463.

400 Histopathology Single Tissue - Mini Report

Specimen Requirements Single Tissue: 1-4 tissue samples from one site (one surgical site or organ type), where length + width + height is less than 15cm in aggregate. Submitted in 10% neutral buffered formalin.

Reported Time 1-3 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Mini report includes gross description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing.

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401 Histopathology Single Tissue - Full Report

Specimen Requirements Single Tissue: 1-4 tissue samples from one site (one surgical site or organ type), where length + width + height is less than 15cm in aggregate. Submitted in 10% neutral buffered formalin.

Reported Time 1-3 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline. Include absorbent material in the outermost bag.

Additional Comments: Full report includes gross description, microscopic description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing.

403 Histopathology Complex Tissue - Full Report

Specimen Requirements Complex Tissue: 1-4 tissue samples from one site (one surgical site or organ type), where length + width + height is greater than 15cm in aggregate. Or each entire organ (examples: brain, heart, lung, spleen, liver, gallbladder, pancreas, 1 kidney, 1 eyeball, 1 ear canal, 1 mammary chain, 1-2 ovaries or testicles). Or each sample requiring decalcification (examples: 1 digit, tail, mandible).

Reported Time 2-5 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar, or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Full report includes gross description, microscopic description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing, while samples with bone or mineralization may require decalcification prior to processing.

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403x2 Histopathology Complex Tissue x2 - Full Report

Specimen Requirements Two 'Complex Tissues'. Complex Tissue = 1-4 tissue samples from one site (one surgical site or organ type), where length + width + height is greater than 15cm in aggregate. Or each entire organ (examples: brain, heart, lung, spleen, liver, gallbladder, pancreas, 1 kidney, 1 eyeball, 1 ear canal, 1 mammary chain, 1-2 ovaries or testicles). Or each sample requiring decalcification (examples: 1 digit, tail, mandible).

Reported Time 2-5 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Full report includes gross description, microscopic description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing, while samples with bone or mineralization may require decalcification prior to processing.

403x3 Histopathology Complex Tissue x3 - Full Report

Specimen Requirements Three 'Complex Tissues'. Complex Tissue = 1-4 tissue samples from one site (one surgical site or organ type), where length + width + height is greater than 15cm in aggregate. Or each entire organ (examples: brain, heart, lung, spleen, liver, gallbladder, pancreas, 1 kidney, 1 eyeball, 1 ear canal, 1 mammary chain, 1-2 ovaries or testicles). Or each sample requiring decalcification (examples: 1 digit, tail, mandible).

Reported Time 2-5 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Full report includes gross description, microscopic description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing, while samples with bone or mineralization may require decalcification prior to processing.

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403x4 Histopathology Complex Tissue x4 - Full Report

Specimen Requirements Four 'Complex Tissues'. Complex Tissue = 1-4 tissue samples from one site (one surgical site or organ type), where length + width + height is greater than 15cm in aggregate. Or each entire organ (examples: brain, heart, lung, spleen, liver, gallbladder, pancreas, 1 kidney, 1 eyeball, 1 ear canal, 1 mammary chain, 1-2 ovaries or testicles). Or each sample requiring decalcification (examples: 1 digit, tail, mandible).

Reported Time 2-5 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Full report includes gross description, microscopic description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing, while samples with bone or mineralization may require decalcification prior to processing.

406 Histopathology Necropsy

Specimen Requirements Refrigerate body, and submit for necropsy, as soon as possible. If refrigeration is not available, pack body with ice packs. Prevent freezing.

Reported Time Preliminary Diagnosis: 1-5 days.Final Diagnosis: 7-14 days, unless special studies are requested.

Collection Notes Please include as much history as possible.

Additional Comments: Please call the Lab for pricing and additional information. Full necropsy includes gross examination, microscopic description, diagnosis, ancillary inhouse lab testing as requested by pathologist, and comments. Gross necropsy is available at a reduced rate when the cause of death is obvious upon gross inspection. Spinal cord examination, toxicology, and cremation services are available at an additional cost.

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407 Histopathology Limb Dissection

Specimen Requirements Refrigerate limb immediately, or store on ice. Submit limb in plastic bags on ice. DO NOT freeze or fix.

Reported Time Preliminary Diagnosis: 3-5 days.Final Diagnosis: 7-14 days.

Collection Notes Please include history and radiographs. Securely seal and label plastic bags, with absorbent material in outermost bag.

Additional Comments: Full report includes gross description, microscopic description, diagnosis, and comments. Reporting time is dependent on soft tissue versus bone analysis.

418 Histopathology Additional Skin Punch Biopsies - Full report

Specimen Requirements Additional 1-4 skin punch biopsies. Submitted in 10% neutral buffered formalin.

Reported Time 1-3 Days, Reviewed M-F

Collection Notes Labelled mesh cassettes in formalin are preferred for small or friable tissues. Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Full report includes gross description, microscopic description, diagnosis, and comments.

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419 Histopathology Additional Single Tissue - Full Report

Specimen Requirements Additional 'Single Tissue'. Additional 1-4 tissue samples from one site (one surgical site or organ type), where length + width + height is less than 15cm in aggregate. Submitted in 10% neutral buffered formalin.

Reported Time 1-3 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Full report includes gross description, microscopic description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing.

419x2 Histopathology Additional Single Tissue x2 - Full Report

Specimen Requirements Two 'Additional Single Tissues' (2 x Additional 1-4 tissue samples from one surgical site or organ type, where length + width + height is less than 15cm in aggregate). Submitted in 10% neutral buffered formalin.

Reported Time 1-3 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Full report includes gross description, microscopic description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing.

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419x3 Histopathology Additional Single Tissue x3 - Full Report

Specimen Requirements Three 'Additional Single Tissues' (3 x Additional 1-4 tissue samples from one surgical site or organ type, where length + width + height is less than 15cm in aggregate). Submitted in 10% neutral buffered formalin.

Reported Time 1-3 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing.When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Full report includes gross description, microscopic description, diagnosis, and comments.Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing.

419x4 Histopathology Additional Single Tissue x4 - Full Report

Specimen Requirements Four 'Additional Single Tissues' (4 x Additional 1-4 tissue samples from one surgical site or organ type, where length + width + height is less than 15cm in aggregate). Submitted in 10% neutral buffered formalin.

Reported Time 1-3 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Full report includes gross description, microscopic description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing.

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423 Histopathology Skin Punch Biopsies - Mini Report

Specimen Requirements 1-4 Skin Punch Biopsies. Submitted in 10% neutral buffered formalin.

Reported Time 1-3 Days, Reviewed M-F

Collection Notes Labelled mesh cassettes in formalin are preferred for small or friable tissues.Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Mini report includes gross description, diagnosis, and comments.

425 Histopathology Skin Punch Biopsies - Full Report

Specimen Requirements 1-4 Skin Punch Biopsies. Submitted in 10% neutral buffered formalin.

Reported Time 1-3 Days, Reviewed M-F

Collection Notes Labelled mesh cassettes in formalin are preferred for small or friable tissues.Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Full report includes gross description, microscopic description, diagnosis, and comments.

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440 Histopathology Additional Skin Punch Biopsies - Mini report

Specimen Requirements Additional 1-4 skin punch biopsies. Submitted in 10% neutral buffered formalin.

Reported Time 1-3 Days, Reviewed M-F

Collection Notes Labelled mesh cassettes in formalin are preferred for small or friable tissues.Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Mini report includes gross description, diagnosis, and comments.

441 Histopathology Additional Single Tissue - Mini Report

Specimen Requirements Additional 'Single Tissue'. Additional 1-4 tissue samples from one site (one surgical site or organ type), where length + width + height is less than 15cm in aggregate. Submitted in 10% neutral buffered formalin.

Reported Time 1-3 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Mini report includes gross description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing.

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441X2 Histopathology Additional Single Tissue x2 - Mini Report

Specimen Requirements Two 'Additional Single Tissues' (2 x Additional 1-4 tissue samples from one surgical site or organ type, where length + width + height is less than 15cm in aggregate). Submitted in 10% neutral buffered formalin.

Reported Time 1-3 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Mini report includes gross description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing.

441X3 Histopathology Additional Single Tissue x3 - Mini Report

Specimen Requirements Three 'Additional Single Tissues' (3 x Additional 1-4 tissue samples from one surgical site or organ type, where length + width + height is less than 15cm in aggregate). Submitted in 10% neutral buffered formalin.

Reported Time 1-3 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Mini report includes gross description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing.

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441X4 Histopathology Additional Single Tissue x4 - Mini Report

Specimen Requirements Four 'Additional Single Tissues' (4 x Additional 1-4 tissue samples from one surgical site or organ type, where length + width + height is less than 15cm in aggregate). Submitted in 10% neutral buffered formalin.

Reported Time 1-3 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Mini report includes gross description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing.

463 Histopathology Complex Tissue - Mini Report

Specimen Requirements Complex Tissue: 1-4 tissue samples from one site (one surgical site or organ type), where length + width + height is greater than 15cm in aggregate. Or each entire organ (examples: brain, heart, lung, spleen, liver, gallbladder, pancreas, 1 kidney, 1 eyeball, 1 ear canal, 1 mammary chain, 1-2 ovaries or testicles). Or each sample requiring decalcification (examples: 1 digit, tail, mandible).

Reported Time 2-5 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Mini report includes gross description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing, while samples with bone or mineralization may require decalcification prior to processing.

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463x2 Histopathology Complex Tissue x2 - Mini Report

Specimen Requirements Two 'Complex Tissues'. Complex Tissue = 1-4 tissue samples from one site (one surgical site or organ type), where length + width + height is greater than 15cm in aggregate. Or each entire organ (examples: brain, heart, lung, spleen, liver, gallbladder, pancreas, 1 kidney, 1 eyeball, 1 ear canal, 1 mammary chain, 1-2 ovaries or testicles). Or each sample requiring decalcification (examples: 1 digit, tail, mandible).

Reported Time 2-5 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Mini report includes gross description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing, while samples with bone or mineralization may require decalcification prior to processing.

463x3 Histopathology Complex Tissue x3 - Mini Report

Specimen Requirements Three 'Complex Tissues'. Complex Tissue = 1-4 tissue samples from one site (one surgical site or organ type), where length + width + height is greater than 15cm in aggregate. Or each entire organ (examples: brain, heart, lung, spleen, liver, gallbladder, pancreas, 1 kidney, 1 eyeball, 1 ear canal, 1 mammary chain, 1-2 ovaries or testicles). Or each sample requiring decalcification (examples: 1 digit, tail, mandible).

Reported Time 2-5 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Mini report includes gross description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing, while samples with bone or mineralization may require decalcification prior to processing.

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463x4 Histopathology Complex Tissue x4 - Mini Report

Specimen Requirements Four 'Complex Tissues'. Complex Tissue = 1-4 tissue samples from one site (one surgical site or organ type), where length + width + height is greater than 15cm in aggregate. Or each entire organ (examples: brain, heart, lung, spleen, liver, gallbladder, pancreas, 1 kidney, 1 eyeball, 1 ear canal, 1 mammary chain, 1-2 ovaries or testicles). Or each sample requiring decalcification (examples: 1 digit, tail, mandible).

Reported Time 2-5 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Mini report includes gross description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing, while samples with bone or mineralization may require decalcification prior to processing.

813 Histopathology Special Stain

Specimen Requirements Paraffin tissue block (preferred) or 2 unstained slides.

Reported Time 1-3 days, Performed M-F

Collection Notes None

Additional Comments: One special stain per one patient submission (up to 5 slides). Pricing based on histopathology previously completed at Phoenix Lab.

Please note: IHC stains are not included in this category. See test code 1667 Immunohistochemistry Stain.

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813x2 Histopathology Special Stain x2

Specimen Requirements Paraffin tissue block (preferred) or 4 unstained slides.

Reported Time 1-3 days, Performed M-F

Collection Notes None

Additional Comments: Two special stains per one patient submission (up to 5 slides per stain). Pricing based on histopathology previously completed at Phoenix Lab.

Please note: IHC stains are not included in this category. See test code 1667 Immunohistochemistry Stain.

813x3 Histopathology Special Stain x3

Specimen Requirements Paraffin tissue block (preferred) or 6 unstained slides.

Reported Time 1-3 days, Performed M-F

Collection Notes None

Additional Comments: Three special stains per one patient submission (up to 5 slides per stain). Pricing based on histopathology previously completed at Phoenix Lab.

Please note: IHC stains are not included in this category. Refer to test code 1667 for Immunohistochemistry Stain.

813x4 Histopathology Special Stain x4

Specimen Requirements Paraffin tissue block (preferred) or 8 unstained slides.

Reported Time 1-3 days, Performed M-F

Collection Notes None

Additional Comments: Four special stains per one patient submission (up to 5 slides per stain). Pricing based on histopathology previously completed at Phoenix Lab.

Please note: IHC stains are not included in this category. Refer to test code 1667 for Immunohistochemistry Stain.

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Microbiology submission guidelines The Phoenix Lab microbiology department is committed to ensuring that veterinarians receive the most accurate, most diagnostically useful results. Please refer to this guide to submit the best samples. Urine Culture Guidelines Minimum volume: 0.5mL Protocol: Whenever possible, urine should be collected via cystocentesis. Samples should be

submitted in a sterile container with no additives. The use of THIO broths in-house to detect positive urines is only acceptable for cystocentesis samples. THIO broths will not be accepted for catheterized or free-catch samples, since the urine is contaminated with low count genital flora. THIOs should only be used for cystocentesis samples. It is strongly recommended that urine be submitted, in addition to a THIO broth, so that the laboratory can quantitate the sample. If THIO broth only is submitted, the sample will not be quantitated.

Cultures are set up 7 days a week and are read daily. Sensitivities are typically reported within 48

hours of culture set up. No growth cultures are finalized after 48 hours of incubation. Special Instructions: Samples should be refrigerated after collection to avoid bacterial overgrowth. Please include method of collection for proper culture interpretation. Sensitivities are run on up to

two uropathogens. Sensitivities on non-Enterococcus Streptococcus are not performed. Sensitivities on isolates #3 and #4 can be requested for an additional charge. Contact the microbiology department to request addition testing.

Please specify if animal has a history of Cushing's or diabetes, or if the animal is on antibiotics or has been on antibiotics recently. This history will help in sample processing where low colony counts are expected.

We accept air dried slides on a case by case basis, typically in instances when the clinic calls bacteria in-house and in-laboratory cultures are no growth. It is a courtesy to the client for education purposes only to help the clinic’s veterinary team better differentiate between bacteria and amorphous debris. Please call the laboratory to request this courtesy testing.

Do not submit samples in LTT. LTT contains EDTA which can be bactericidal. DO NOT submit syringes with needles. Fecal Flotation Minimum sample size: 5g (about the size of a walnut). Less may lead to a false negative result. Protocol: Feces in blue screw top fecal contain (contact laboratory to request container) Sugar flotation is performed to rule out intestinal parasites. To rule out Salmon Poisoning, request test code #213. Combo test codes are available which include Giardia/Crypto IFA or Giardia Elisa. Specimens are run daily in AM and PM. Samples are not run on Sundays. Special Instructions: Whenever possible, please submit samples in blue screw top fecal container (provided by laboratory

upon request).

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Aerobic and/or Anaerobic Culture Specimen Collection Protocol: Feces: Culturette or fresh feces in container. Request test code #519 to rule out Salmonella and

Campylobacter. Yersinia and Shigella can be ruled out upon request. FNA/SQ Fluid: Submit fluid in a sterile container. MRSA Screen: Culturette of nasal/perirectal area. Request test code #505. Must specify “Rule out

MRSA” on test request form. Respiratory washes (TTW, BAL): Submit fluid in a sterile container. Skin/Ear: Culturette of affected area. Tissue/Biopsy: Tissue in sterile container with a drop of sterile saline Aerobic and anaerobic cultures are set up 7 days a week and are read daily. Sensitivities are not

performed on anaerobic organisms. Sensitivities on aerobic pathogens are typically reported within 72 hours of culture set up. No growth cultures are finalized after 5 days of incubation.

Special Instructions: Do not submit samples in LTT. LTT contains EDTA which can be bactericidal. Samples should be refrigerated after collection to avoid bacterial overgrowth. Please specify if the animal is on antibiotics or has been on antibiotics recently. DO NOT submit syringes with needles. DO NOT submit culture if Brucella is suspected. Contact laboratory for appropriate testing to rule

out Brucella. DO NOT submit culture if animal is known to have a positive Brucella screen. Fungal Culture Specimen Collection Protocol: Skin/Hair/Nail Dermatophyte: Skin scrapings or hair pluckings of affected area submitted in a sterile

container. All other samples: Submit fluid or Culturette of affected area Fungal cultures are read 1 day a week on Sundays. Cultures are finalized after 4 weeks of incubation. Special Instructions: In-house DMT slants can be submitted for culture; however, it is strongly recommended that the

original skin or hair sample also be submitted to rule out the possibility of a false negative. DO NOT submit cultures if any of the following organisms are suspected: Blastomyces Coccidiodes Histoplasma Contact the laboratory for appropriate testing to rule out these organisms.

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Microbiology

501 Acid Fast Stain

Methodology: Stain, Microscopic Exam

Specimen Requirements Bacterial culture swab with transport medium, tissue, feces, mucosal scraping, aspirate OR air-dried slides prepared with a thin film of one of the above.

Reported Time Next day

Collection Notes None

Additional Comments: Positive results reported as Acid fast bacilli observed.

533 Add-On, Sensi/Yeast/Topicals

Methodology: Sensitivity, manual

Specimen Requirements Isolate in Laboratory - Call Client Services to Request Add-On testing

Reported Time 24-36 hours

Collection Notes None

Additional Comments: *Must call Client Service within 48 hours of receiving final culture results.*

Additional Sensitivity: This is an add-on test to an Aerobic Culture (505 or 500) for 3rd and 4th culture isolates. Contact the laboratory to request this test. Samples are run on Vitek 2 instrument. Sensitivities should be requested within 48 hours of final report. Topicals: This is an add-on test to an Aerobic Culture (505 or 500). Contact the laboratory to request this test. Sensitivities should be requested within 48 hours of final report. Sensitivities are tested manually and do not include MICs. Test includes topical medications for up to 2 isolates (neomycin, tobramycin, polymyxin B, ciprofloxacin, orbifloxacin). Injectables: This is an add-on test to an Aerobic Culture (505 or 500). Contact the laboratory to request this test. Sensitivities should be requested within 48 hours of final report. Test includes injectable medications for up to 2 isolates (aztreonam, ceftazidime, cefepime, cefotaxime, ceftriaxone, ciprofloxacin, levofloxacin, piperacillin, piperacillin/tazobactam, and tobramycin). Yeast ID: This is an add-on test to an Aerobic Culture (505 or 500) when yeast has been isolated. Identification is run in-house on Vitek 2 instrument. If unable to identify via in-house methodology, Send Out options are available for an additional charge.

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505 Culture & Sensitivity, Aerobic

Methodology: Culture

Specimen Requirements Bacterial culture swab with transport medium or fluid/tissue in CTT or sterile tube

Reported Time 2-5 Days

Collection Notes Please indicate culture site and animal species for proper processing. If submitting tissue, moisten with small amount of sterile saline (free of preservative) if piece of tissue is small enough to dehydrate en route to laboratory.

Additional Comments: Sensitivity and gram stain included. Do not submit samples in lavender top tube; EDTA is bacteriostatic.

516 Culture & Sensitivity, Milk

Methodology: Culture

Specimen Requirements 1 mL milk in CTT or sterile tube.

Reported Time 2-5 days

Collection Notes None

Additional Comments: Sensitivity and gram stain included. Do not submit samples in lavender top tube; EDTA is bacteriostatic.

524 Culture ID/Sensitivity if Indicated, Avian

Methodology: Culture

Specimen Requirements Bacterial culture swab with transport medium or fluid/tissue in CTT or sterile tube

Reported Time 2-5 Days

Collection Notes None

Additional Comments: For the detection of gram negative rods in psittacines (parrots). Sensitivities will be performed on each isolate for an additional charge per isolate.

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512 Culture, Aerobic ID Only

Methodology: Culture

Specimen Requirements Bacterial culture swab with transport medium or fluid/tissue in CTT or sterile tube

Reported Time 2-5 Days

Collection Notes Please indicate culture site and animal species for proper processing. If submitting tissue, moisten with small amount of sterile saline (free of preservative) if piece of tissue is small enough to dehydrate en route to laboratory.

Additional Comments: Sensitivity and gram stain included. Do not submit samples in lavender top tube; EDTA is bacteriostatic.

514 Culture, Aerobic ID only, Anaerobic  ID only

Methodology: Culture

Specimen Requirements Bacterial culture swab with transport medium or fresh tissue in sterile CTT.

Reported Time 2-5 Days

Collection Notes Please indicate culture site and animal species for proper processing. If submitting tissue, moisten with small amount of sterile saline (free of preservative) if piece of tissue is small enough to dehydrate en route to laboratory.

Additional Comments: None

500 Culture, Aerobic w/ Sensitivity/Anaerobic Culture, ID only

Methodology: Culture

Specimen Requirements Bacterial culture swab with transport medium or fresh tissue in sterile CTT

Reported Time 2-5 Days

Collection Notes Please indicate culture site and animal species for proper processing. If submitting tissue, moisten with small amount of sterile saline (free of preservative) if piece of tissue is small enough to dehydrate en route to laboratory.

Additional Comments: Aerobic culture includes sensitivities on up to 2 isolates. Anaerobic sensitivities are not available. Includes gram stain. Do not submit samples in lavender top tube; EDTA is bacteriostatic.

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502 Culture, Anaerobic (ID Only)

Methodology: Culture

Specimen Requirements Bacterial culture swab with transport medium or fluid/tissue in CTT or sterile tube

Reported Time 2-5 Days

Collection Notes Please indicate culture site and animal species for proper processing. If submitting tissue, moisten with small amount of sterile saline (free of preservative) if piece of tissue is small enough to dehydrate en route to laboratory.

Additional Comments: Anaerobic sensitivities are not available. Do not submit samples in lavender top tube; EDTA is bacteriostatic.

503 Culture, Blood

Methodology: Culture

Specimen Requirements Two aerobic blood culture bottles should be submitted (available upon request). Blood should be drawn from two sites (i.e. left jugular, right saphenous)

Reported Time 24 hr preliminary report, Final at 7 days

Collection Notes 1. Label each blood culture bottle with the date, time, and draw site. Blood should be drawn from two sites (i.e. left jugular, right saphenous), at least one hour apart.2. Thoroughly disinfect blood draw sites (shave if needed). Inoculate the bottles with up to 3mL of blood. NOTE: If less than 3mL is used for inoculation, it may take longer for the blood culture to be detected as positive. 3. After inoculation, the bottles can be incubated at 37 degrees Celsius, or left at room temperature until pick up. DO NOT REFRIGERATE.

Additional Comments: Culture includes susceptibility panels for 2 pathogens. Bottles are incubated for 7 days and subcultured at regular intervals.

507 Culture, Dermatology

Methodology: Culture

Specimen Requirements Bacterial culture swab with transport medium or fluid/tissue in CTT or sterile tube

Reported Time 2-5 Days

Collection Notes None

Additional Comments: Sensitivity and gram stain included. Do not submit samples in lavender top tube; EDTA is bacteriostatic.Includes standard sensitivity profile plus topical medications (neomycin, tobramycin, polymyxin B, ciprofloxacin, and orbifloxacin). Up to two isolates are included.

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508 Culture, Equine Uterine

Methodology: Culture

Specimen Requirements Bacterial culture swab with transport medium

Reported Time 2-5 Days

Collection Notes None

Additional Comments: Normal test value is "No Beta-hemolytic streptococci isolated."

Refer to test #862 Equine Uterine Screen for combo test with uterine cytology.

519 Culture, Feces

Methodology: Culture

Specimen Requirements 5 grams of Fresh Feces, Fecal Tube or bag.

Reported Time 36 - 72 hours

Collection Notes None

Additional Comments: Fecal culture for the detection of Salmonella and Campylobacter. Shigella and Yersinia can be ruled out upon request. Additional charges may apply.

504 Culture, Fungal

Methodology: Culture

Specimen Requirements Hair or nail clippings in CTT, skin scrapings in CTT, bacterial culture swab with transport medium, fluid in CTT or sterile tube, or fresh tissue in CTT or sterile tube accepted.

Reported Time Preliminary reported at 1 week. Cultures are held for up to 4 weeks.

Collection Notes Please include culture site and animal species for proper processing. If submitting tissue, moisten with small amount of sterile saline (free of preservative) if piece of tissue is small enough to dehydrate en route to laboratory.

Additional Comments: Updates reported weekly on Sunday.

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618 Culture, Mycoplasma

Methodology: Culture

Specimen Requirements Swab in Mycoplasma Transport Media, exudates from: eye, nostril, trachea, synovial fluid and/or genitals or fluid in CTT or sterile tube.

Reported Time Preliminary report at 1 week. Finalized after 2 weeks.

Collection Notes Sample collection should be made with synthetic swabs. Cotton tips and wooden stick swabs inhibit growth of Mycoplasma. Media should be frozen or refrigerated before and after collection. Transport to the laboratory as soon as possible.

Additional Comments: Mycoplasma sensitivities are not available. To rule out Ureaplasma, See our Sendout catalog, Ureaplasma Culture SO111.

506 Culture, Urine

Methodology: Culture

Specimen Requirements 0.5 mL Urine, Urine Tube/CTT.

Reported Time 36 - 72 hours, No Growth cultures finalized after 48 hours.

Collection Notes Whenever possible, urine should be collected by cystocentesis. If not possible, collect catheterized or free catch urine sample into sterile container. Refrigerate urine sample after collection.

Additional Comments: Colony counts are not possible if a plate, broth, or swab is submitted. Culture includes sensitivities on up to 2 isolates. Cultures are quantitated and reported in CFU/mL. No growth cultures are finalized after 48 hours of incubation.

To include with a urinalysis, refer to Urinalysis with Culture 548.To add on to previous urine test, refer to Urine Culture Add-on 549.

523 Dermatophilus Prep

Methodology: Microscopic Examination

Specimen Requirements Skin scrapings or skin crusts submitted in a CTT or sterile container

Reported Time Next day

Collection Notes None

Additional Comments: Dermatophilus Prep should be used as a quick screen only. If prep is negative and Dermatophilus is suspected, it is recommended to run an Aerobic Culture, ID only, test code 512, before ruling out Dermatophilus congolensis as source of infection.

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236 Fecal Baermann

Methodology: Sedimentation

Specimen Requirements 3-5 grams of Fresh Feces, Fecal Tube or bag. For most accurate results, please submit fresh feces (less than 24 hours old).

Reported Time Next day

Collection Notes None

Additional Comments: Also known as Lungworm Fecal Baermann, this is a qualitative test, reporting presence or absence of lungworm larvae. This test does not rule out Filaroides sp. (Canine Lungworm).

To rule out Filaroides, submit bronchoalveolar lavage or transtracheal wash for cytology (318) or fecal sample for Ova and Parasite (213).

2241 Fecal Combo Screen w/ Giardia ELISA

Fecal Screen

Specimen Requirements 10 grams of Fresh Feces, Fecal Tube or bag.

Reported Time Next Day

Giardia ELISA

Collection Notes None

Additional Comments: Test includes sugar flotation with two centrifugation sequences; microscopic examination for ova and parasites, including Giardia cysts. Giardia ELISA reported out same day, fecal results out next day.

2409 Fecal Parasite Combo w/ Giardia/Crypto IFA

Fecal Screen

Specimen Requirements 10 grams of Fresh Feces, Fecal Tube or bag. Sample can not contain clay litter.

Reported Time See Comment

Giardia/Crypto IFA

Collection Notes Do not collect sample contaminated with clay kitty litter. It causes interference with IFA testing. Wheat/corn litter OK.

Additional Comments: Fecal float includes sugar flotation with two centrifugation sequences; microscopic examination for ova and parasites, including Giardia cysts. IFA testing will detect the antigen of Giardia species and most Cryptosporidia.It will not detect some species of Cryptosporidium that are associated with birds and reptiles.

For avian/non-mammalian testing, order Avian/Non-Mammalian Giardia/Cryptosporidium 1530.

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409 Fecal Parasite Screen

Methodology: Centrifugation/Flotation

Specimen Requirements 5-10 grams of Fresh Feces, Fecal Tube or bag. For most accurate results, please submit fresh feces (less than 24 hours old).

Reported Time Next day

Collection Notes None

Additional Comments: Test includes sugar flotation with two centrifugation sequences; microscopic examination for ova and parasites, including Giardia cysts. Giardia/Crypto IFA (241) is recommended to confirm the presence of Giardia as noted.

1409 Fecal Parasite, Quantitative

Methodology: Centrifugation/Microscopic

Specimen Requirements 1.0 gram Fresh Feces, Fecal tube or bag

Reported Time Next Day

Collection Notes None

Additional Comments: Results will be reported out as type of parasite and number of eggs per gram of feces.

509 Gram Stain

Methodology: Microscopic Examination

Specimen Requirements Submit air-dried slides, swab, fluid or tissue.

Reported Time Next day

Collection Notes Collection site/source and species required for proper interpretation.

Additional Comments: Normal test values vary with culture site.

530 Modified Acid Fast Stain

Methodology: Microscopic Examination

Specimen Requirements Bacterial culture swab with transport medium, tissue, feces, mucosal scraping, aspirate OR air-dried slides prepared with a thin film of one of the above.

Reported Time Next Day

Collection Notes None

Additional Comments: Also known as modified Kinyoun Acid Fast Stain. For the detection of Nocardia species.

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213 Ova & Parasite

Methodology: Centrifugation/Microscopic

Specimen Requirements 5 grams Fresh Feces, Fecal Tube or bag

Reported Time Next day

Collection Notes None

Additional Comments: Test includes a direct smear as well as a sugar flotation with two centrifugation sequences; microscopic examination for ova and parasites, including Giardia cysts. Giardia/Crypto IFA add-on #241 is recommended to confirm the presence of Giardia as noted.

2213 Ova & Parasite Combo w/IFA

Ova & Parasite

Specimen Requirements 10 grams of Fresh Feces, Fecal Tube or bag. Sample can not contain clay litter.

Reported Time Next Day

Giardia/Crypto IFA

Collection Notes Do not collect sample contaminated with clay kitty litter. It causes interference with IFA testing. Wheat/corn litter OK.

Additional Comments: Ova and parasite test includes a direct smear as well as a sugar flotation with two centrifugation sequences; microscopic examination for ova and parasites, including Giardia cysts. IFA testing will detect the antigen of Giardia species and most Cryptosporidia. It will not detect some species of Cryptosporidium that are associated with birds and reptiles.

For avian/non-mammalian testing, see Avian/Non-Mammalian Giardia/Cryptosporidium 1530.

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1213 Tritrichomonas Fecal

Methodology: Culture

Specimen Requirements 0.05 grams Fecal in InPouch TF or Fecal tube. The sample for this test should NOT be refrigerated or put in a cooler.

Reported Time 24 hr preliminary report Final at 12 Days

Collection Notes Please submit your sample in a zip-lock bag labelled "Do Not Refrigerate". Cold temperatures will destroy the sample for testing.Pouch Procedure: 1. Pull open wired end-tabs, tearing open the pouch at the notch. 2. Open pouch sufficiently to admit a wooden applicator stick by pulling the closure tape's middle tabs apart.3. Pick-up approximately 0.05 grams, the size of a small peppercorn, or fresh feces on applicator stick(s).4.Insert applicator stick(s) with sample into the liquid of the system's upper chamber. 5. Remove specimen from stick by rubbing stick with fingers through the walls of the pouch. 6. Seal the pouch and store at room temperature in a dark place until submission to laboratory for interpretation.

Additional Comments: InPouch TF is available from Phoenix Laboratory as a CHARGEABLE supply.

548 Urinalysis w/ C&S

Urinalysis

Specimen Requirements 5 mL Urine, Urine Tube/CTT. For the most accurate results 1 mL urine in sterile container should be submitted in addition to 5 mL urine.

Reported Time See Comment

Urine Culture

Collection Notes Please indicate method of urine collection.Do not submit syringes with needles, they are not accepted. Whenever possible, urine should be collected by cystocentesis. When this is not possible, free-catch urine into sterile container.Submit sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Turn around time is same day for the UA and 36 - 72 hours for Culture. Culture includes sensitivity panels for 2 pathogens. Additional sensitivities can be requested for an additional charge. No growth cultures are finalized after 48 hours of incubation. Urine collected on a swab or thio will not allow a colony count to be provided.

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552 Urinalysis w/ Culture, Follow-up

Urinalysis

Specimen Requirements 5 mL Urine, Urine Tube/CTT. For the most accurate results 1 mL urine in sterile container should be submitted in addition to 5 mL urine.

Reported Time See Comments

Urine Culture

Collection Notes Please indicate method of urine collection.Do not submit syringes with needles, they are not accepted. Whenever possible, urine should be collected by cystocentesis. When this is not possible, free-catch urine into sterile container.Submit sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Please include all antibiotic history with urine culture.Turn around time is same day for the UA and 36 - 72 hours for Culture. Culture includes sensitivity panels for 2 pathogens. Additional sensitivities can be requested for an additional charge. No growth cultures are finalized after 48 hours of incubation. Urine collected on a swab or thio will not allow a colony count to be provided.

Submit within 30 days of original test. Previous Case ID is required to activate the discounted price

549 Urine Culture & Sensitivity, Add-On

Methodology: Culture

Specimen Requirements 0.5 mL Urine, Urine Tube/CTT.

Reported Time 36 - 72 hours

Collection Notes Whenever possible, urine should be collected by cystocentesis. If not possible, collect catheterized or free catch urine sample into sterile container. Refrigerate urine sample after collection.

Additional Comments: Colony counts are not possible if a plate, broth, or swab is submitted. Culture includes sensitivities on up to 2 isolates. Cultures are quantitated and reported in CFU/mL. No growth cultures are finalized after 48 hours of incubation.

Available as an add-on to an existing panel only (within 7 days). Previous case ID is required to activate the discounted price.

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551 Urine Culture, Follow-up

Methodology: Culture

Specimen Requirements 1.0 mL Urine, CTTCollected via cystocentesis preferred

Reported Time 36-72 hours

Collection Notes Whenever possible, urine should be collected by cystocentesis. If not possible, collect catheterized or free catch urine sample into sterile container. Refrigerate urine sample after collection.

Additional Comments: Please include all antibiotic history with urine culture.Colony counts are not possible if a plate, broth, or swab is submitted. Culture includes sensitivities on up to 2 isolates. Cultures are quantitated and reported in CFU/mL. No growth cultures are finalized after 48 hours of incubation.

Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.To include with a urinalysis, refer to Urinalysis with Culture, Follow-up 552.

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Test List, Alphabetical

3336 Absolute Reticulocyte

Methodology: Flow Cytometry

Specimen Requirements 0.5 mL Whole Blood, LTT

Reported Time Same Day

Collection Notes None

Additional Comments: This is included in a CBC when a canine or feline hematocrit is < 30%.This test includes a retic %, RBC and an absolute retic.If the canine absolute reticulocyte count is >150,000/uL, the anemia is considered regenerative.If the feline absolute reticulocyte count is >50,000/uL, the anemia is considered regenerative.

501 Acid Fast Stain

Methodology: Stain, Microscopic Exam

Specimen Requirements Bacterial culture swab with transport medium, tissue, feces, mucosal scraping, aspirate OR air-dried slides prepared with a thin film of one of the above.

Reported Time Next day

Collection Notes None

Additional Comments: Positive results reported as Acid fast bacilli observed.

697 ACTH Plasma, Endogenous

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL EDTA Plasma, Separated and Frozen, CTT

Reported Time Mon/Wed/Fri, Same day

Collection Notes Spin down LTT immediately and separate from cells. Freeze as soon as possible. Frozen plasma stable for 14 days. Avoid grossly hemolyzed and/or lipemic samples

Additional Comments: Dexamethasone suppression test is no longer recommended as the primary test for diagnosis of PPID/Cushing's Disease in horses.  Endogenous ACTH is the preferred test.  Also see test code 6971 for information about TRH stimulation testing.

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39 ACTH Response

Methodology: Chemiluminescent Immunoassay

Pre Cortisol

Specimen Requirements 0.5 mL Serum each RTTx2; Pre, 1 hour Post. DO NOT USE SST TO COLLECT OR SPIN DOWN SAMPLE

Reported Time Same Day

Post Cortisol

Collection Notes PROTOCOL:1.  Collect a resting sample in a RTT, 0.5 mL of serum is required.  Separate serum if possible and refrigerate. 2.  Inject 0.25 mg (250 µg) of Cortrosyn IM or IV (1 vial). 3.  Collect a second sample EXACTLY 1 HOUR post injection in RTT, label clearly as to the collection status (post), and preserve in the same manner as the first sample.

NOTE: The test has been validated using Cortrosyn. Although compounded gels are available, they vary greatly in peak times and are not recommended.

ALTERNATE CANINE PROTOCOL (Microdose): In order to keep Cortrosyn costs down, use the specific dose of 5 ug/kg IV - up to the maximum dose of 250 ug (one vial) per dog.  Whatever is left over can then be split into aliquots of 25 ug  or 50 ug using small plastic syringes.  Normally 1 mL of sterile physiologic saline is used as diluent.  If so, 25 ug would be 100 uL or 0.1 mL. ***When the patient is receiving Trilostane/Vetoryl, the timing of the test is important.  The pre-cortisol sample should be drawn 3-5 hours after the morning capsule.  The test is a one hour test, so in other words, the ACTH Response test should be completed 4-6 hours after the medication, which is usually given once a day in the morning.

Additional Comments: Pre and Post sample required. In addition to diagnosis of naturally occurring hyperadrenocorticism (Cushing's disease), this is the only diagnostic test for hypoadrenocorticism (Addison's disease), iatrogenic hyperadrenocorticism, and monitoring response to medical therapy of Cushing's disease. Prednisone, prednisolone, and other corticosteroids (except dexamethasone) will cross react. Recommend these drugs be discontinued at least 48 hrs before testing. If dog has been on prolonged steroids, patient must be tapered with the drug stopped for at least 3 weeks to let the adrenal gland recover. INTERPRETATION HYPERADRENOCORTICISM: An ACTH response test is done to CONFIRM clinical suspicion of hyperadrenocorticism. Stressful diseases (ill diabetics, renal failure, etc.) and current drug therapy may cause false positives. If the cortisol results are normal (and an adequate dose of Cortrosyn was administered) and there is high clinical suspicion of hyperadrenocorticism, sex hormone hyperadrenocorticism (atypical Cushing's) should be considered. Additional testing on the serum from this ACTH stimulation test can be performed. Please contact us within 7 days of this cortisol result for further testing options. A low-dose dexamethasone suppression test should also be considered, as some patients with Cushing's will be positive on one test but not on the other.INTERPRETATION HYPOADRENOCORTICISM: Pre and post cortisol <2 ug/dL are supportive of hypoadrenocorticism but need to be interpreted in light of other clinical findings and history. Failure to stimulate can occur with iatrogenic hyperadrenocorticism, dogs being treated for hyperadrenocorticism and Addison's disease.

For Feline Protocol See ACTH Response, Feline 1039

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1039 ACTH Response, Feline

Methodology: Chemiluminescent Immunoassay

Pre Cortisol

Specimen Requirements 3 RTT Minimum:  0.25 mL for each sampleAvoid severe lipemia

Reported Time Same Day

30 min Post Cortisol 60 min Post Cortisol

Collection Notes FELINE PROTOCOL: DO NOT USE SST TO COLLECT OR SPIN DOWN SAMPLE1.  Collect a resting sample in a RTT, 0.5 mL of serum is required.  Separate serum if possible and refrigerate. 2.  Inject 0.125 mg  of Cortrosyn  IM or IV, (1/2 vial). 3.  Collect a sample exactly 30 min and 60 min post injection in a RTT, label clearly as to the status (post-30, post-60), and preserve in the same manner as the first sample.

Additional Comments: Feline hyperadrenocorticism is very rare, we suggest consulting with an internist/clinical pathologist at the Laboratory prior to diagnostic testing. Dexamethasone suppression test is the favored diagnostic test for hyperadrenocorticism in cats. ACTH Response test is not recommended.NOTE: The test has been validated using Cortrosyn. Although compounded gels are available, they vary greatly in peak times and are not recommended. Prednisone, prednisolone, and other corticosteroids (except dexamethasone) will cross react. Recommend these drugs be discontinued at least 48 hrs before testing. If the patient has been on steroids >14 days, patient must be tapered and discontinue steroids for at least two weeks prior to testing.

343 Activated Partial Thromboplastin Time (APTT)

Methodology: Endpoint Clot Detection

Specimen Requirements 0.5 mL Citrated PlasmaSee Collection notes for details about proper BTT volume.

Reported Time Same Day

Collection Notes Citrated tubes (BTT) provided by Phoenix Laboratory hold 2.7 mL of blood (to the top of the label). Tubes must be filled with 2.7 mL of blood. If collecting a smaller volume of blood (<2.7mL) the blood to anticoagulant ratio should be 9:1. Please call lab with any questions.Protocol: 1. Draw fresh venous blood into BTT until full (to top of label) but not beyond the top of the tube label. No clotting should be observed in the tube. 2. Cool to 4° C (refrigeration) until analysis. 3. If a delay greater than 24 hours is expected prior to analysis, then the BTT should be spun in a centrifuge, the plasma separated and placed in a CTT or other non-additive tube to be frozen until analysis.

Additional Comments: The ratio of blood to anticoagulant is critical for valid coagulation results. Any clotting will results in the tube being rejected for analysis.

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533 Add-On, Sensi/Yeast/Topicals

Methodology: Sensitivity, manual

Specimen Requirements Isolate in Laboratory - Call Client Services to Request Add-On testing

Reported Time 24-36 hours

Collection Notes None

Additional Comments: *Must call Client Service within 48 hours of receiving final culture results.*

Additional Sensitivity: This is an add-on test to an Aerobic Culture (505 or 500) for 3rd and 4th culture isolates. Contact the laboratory to request this test. Samples are run on Vitek 2 instrument. Sensitivities should be requested within 48 hours of final report. Topicals: This is an add-on test to an Aerobic Culture (505 or 500). Contact the laboratory to request this test. Sensitivities should be requested within 48 hours of final report. Sensitivities are tested manually and do not include MICs. Test includes topical medications for up to 2 isolates (neomycin, tobramycin, polymyxin B, ciprofloxacin, orbifloxacin). Injectables: This is an add-on test to an Aerobic Culture (505 or 500). Contact the laboratory to request this test. Sensitivities should be requested within 48 hours of final report. Test includes injectable medications for up to 2 isolates (aztreonam, ceftazidime, cefepime, cefotaxime, ceftriaxone, ciprofloxacin, levofloxacin, piperacillin, piperacillin/tazobactam, and tobramycin). Yeast ID: This is an add-on test to an Aerobic Culture (505 or 500) when yeast has been isolated. Identification is run in-house on Vitek 2 instrument. If unable to identify via in-house methodology, Send Out options are available for an additional charge.

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1719 Adult Routine Screen

CBCAlbuminALP (Alkaline Phosphatase)BUN (Blood Urea Nitrogen)CreatinineUrinalysis

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urine

Reported Time Same Day

BUN/Creat RatioGlobulinGlucosePotassiumALT (Alanine Transaminase)

Total ProteinAlbumin/Globulin ratioChlorideSodiumSodium/Potassium Ratio

Collection Notes Please indicate method of urine collection. Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Submit sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: None

101 Albumin

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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102 Alkaline phosphatase (ALP)

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

129 ALT (Alanine Transaminase)

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

894 ALT/AST

Methodology: Spectrophotometry

Specimen Requirements 0.3 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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103 Amylase

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

62 Amylase and Lipase, Add-On

Methodology: Spectrophotometry

Specimen Requirements 0.5 mL Serum, SST/RTT (minimum: 0.2 mL serum)

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Available as an add-on to an existing panel (within 7 days). Previous case ID is required to activate the discounted price.

22 Amylase/Lipase

Methodology: Spectrophotometry

Specimen Requirements 0.5 mL Serum, SST/RTT. Minimum: 0.2 mL Serum

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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602 Antinuclear Antibody

Methodology: IFA

Specimen Requirements 0.5 mL Serum, SST/RTT. Minimum: 0.2 mL Serum. EDTA/heparinized plasma is OK.

Reported Time Tuesday/Thursday, Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: If positive, results will be held and a confirmatory titer will be run Wednesday/ Friday.Steroids will affect this test result.

242 Antinuclear Antibody, Add-On

Methodology: IFA

Specimen Requirements 0.5 mL Serum, SST/RTT. Minimum: 0.2 mL Serum. EDTA/heparinized plasma is OK.

Reported Time Tuesday/Thursday, Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: If positive, results will be held for a confirmatory titer that will be run Wednesday/Friday.Steroids will affect this test result. Available as an add-on to an existing panel (within 7 days). Previous case ID is required to activate the discounted price.

128 AST (Aspartate Transaminase)

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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52 Autoimmune Panel

CBCPlatelet Count

Specimen Requirements ANA: 0.5 mL Serum, SST/RTTCBC and Coombs: 1.5 mL whole blood LTT(1.0 mL minimum) and air dried peripheral blood smears

Reported Time See Comment

Coombs ANA (Antinuclear Antibody)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Corticosteroids can cause false negative Coombs results, therefore consider discontinuing corticosteroid use 2-3 weeks prior to testing.

Additional Comments: Coombs performed Mon-Sat on Days. ANA is performed Tues/Thurs AM. A positive ANA will be held and is titered the next day (Wed/Fri)

Steroids will affect this test result, however, studies have shown that corticosteroid therapy of <7 days do not cause false negatives.

3003 Avian Liver Chemistry

Methodology: Spectrophotometry

AlbuminAST (Aspartate Transaminase)

Specimen Requirements 0.3 mL heparinized plasma (minimum 100 µL) or 250 µL whole blood, m-GrnTTPlease call our Client Services for information about minimums on short samples.

Reported Time Same Day

CholesterolCK (Creatine Kinase)

Bile Acids

Collection Notes None

Additional Comments: None

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1187 Avian Mini Liver Panel

Methodology: Spectrophotometry

Total ProteinAlbumin

Specimen Requirements 0.2 mL Serum, m-SST/m-RTT (Minimum: 0.1 mL)Heparinized plasma or 0.25 mL whole blood from m-GrnTT is also accepted.

Reported Time Same Day

CK (Creatine Kinase)Bile Acids

AST (Aspartate Transaminase)GGT (Gamma-glutamyltransferase)

Collection Notes None

Additional Comments: None

5024 Avian Mini Renal Panel

Methodology: Spectrophotometry

Uric AcidPhosphorous

Specimen Requirements 0.2 mL Serum m-SST/m-RTT (minimum: 0.1 mL Serum). Heparinized plasma or 250 µL whole blood from m-GrnTT is also accepted.

Reported Time Same day

BUN (Blood Urea Nitrogen) CalciumCa/Phos Ratio

Collection Notes None

Additional Comments: None

7 Avian Panel

WBC Est/DiffHematocrit (HCT)GlucoseCalcium

Specimen Requirements Chemistry: 0.3 mL (minimum: 100 µL), heparinized plasma or 250 µL Whole Blood, m-GrnTTCBC: 2 air dried slides and 0.1 mL (Absolute minimum: 30 µL) of either heparinized or EDTA whole blood. Heparin preferred for some species (e.g. Crows, Jays)Please call our Client Services for information about minimums on short samples.

Reported Time Same Day

Total ProteinAST (Aspartate Transaminase)CK (Creatine Kinase)Cholesterol

Uric AcidBile AcidPhosphorusCa/Phos Ratio

Collection Notes One m-GrnTT can be used, volume allowing. Submit m-GrnTT without centrifugation in order for HCT and WBC to be done on same tube. Prepare Slides at clinic.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: If a microtainer (M-GrnTTor M-LTT) is not submitted, WBC is estimated from the blood smear.

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82 Avian Panel, Follow-Up

WBC Est/DiffHematocrit (HCT)GlucoseCalcium

Specimen Requirements Chemistry: 0.3 mL (minimum: 100 µL), heparinized plasma or 250 µL Whole Blood, m-GrnTTCBC: 2 air dried slides and 0.1 mL (Absolute minimum: 30 µL) of either heparinized or EDTA whole blood. Heparin preferred for some species (e.g. Crows, Jays)Please call our Client Services for information about minimums on short samples.

Reported Time Same Day

Total ProteinAST (Aspartate Transaminase)CK (Creatine Kinase)Cholesterol

Uric AcidBile AcidPhosphorusCa/Phos Ratio

Collection Notes One m-GrnTT can be used, volume allowing. Submit m-GrnTT without centrifugation in order for HCT and WBC to be done on same tube. Prepare Slides at clinic.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: If a microtainer (M-GrnTT or M-LTT) is not submitted, WBC is estimated from the blood smear. Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

3002 Avian Renal Chemistry

Methodology: Spectrophotometry

BUN (Blood Urea Nitrogen)CalciumPhosphorus

Specimen Requirements 0.3 mL heparinized Plasma (minimum 100 µL) or 250 µL Whole Blood, m-GrnTTPlease call our Client Services for information about minimums on short samples.

Reported Time Same Day

Ca/Phos RatioTotal ProteinAlbumin

GlobulinA/G RatioUric Acid

Collection Notes None

Additional Comments: None

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3004 Avian/ Nonmammalian 13 Chemistry Screen

Methodology: Spectrophotometry

GlucoseCalciumPhosphorusCa/Phos RatioTotal Protein

Specimen Requirements 0.3 mL heparinized Plasma (Minimum: 150µL) or 350 µL Whole Blood, m-GrnTTPlease call our Client Services for information about minimums on short samples.

Reported Time Same Day

AlbuminGlobulinA/G RatioAST (Aspartate Transaminase)

CK (Creatine Kinase)CholesterolUric AcidBile Acids

Collection Notes None

Additional Comments: None

907 Avian/Nonmammalian 10-Chemistry Screen

Methodology: Spectrophotometry

GlucoseCalciumTotal ProteinCK (Creatine Kinase)

Specimen Requirements 0.3 mL (minimum: 100 µL) heparinized plasma or 250 µL Whole Blood, m-GrnTTPlease call our Client Services for information about minimums on short

Reported Time Same Day

CholesterolUric AcidBile Acids

AST (Aspartate Transaminase)PhosphorusCa/Phos Ratio

Collection Notes None

Additional Comments: None

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3005 Avian/Nonmammalian 13 Chemistry w/ CBC

CBCGlucoseCalciumPhosphorusCa/Phos Ratio

Specimen Requirements Chemistry: 0.3 mL (minimum 150 µL), heparinized plasma or 350 µL Whole Blood, m-GrnTTCBC: 2 air dried slides and 0.1 mL (Absolute minimum: 30 µL) of either heparinized or EDTA whole blood. Heparin preferred for some species (e.g. Crows, Jays)Please call our Client Services for information about minimums on short samples.

Reported Time Same Day

Total ProteinAlbuminGlobulinA/G RatioAST (Aspartate Transaminase)

CK (Creatine Kinase)CholesterolUric AcidBile Acids

Collection Notes One m-GrnTT can be used, volume allowing. Submit m-GrnTT without centrifugation in order for HCT and WBC to be done on same tube. Prepare Slides at clinic.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: If a microtainer (m-GrnTT or m-LTT) with whole blood is not submitted, WBC is estimated from the blood smear and PCV will be cancelled.

3006 Avian/Nonmammalian 13 Chemistry w/ CBC and PEP

CBCGlucoseCalciumPhosphorusCa/Phos Ratio

Specimen Requirements Chemistry: 0.3 mL (minimum 175 µL), heparinized plasma or 350 µL Whole Blood, m-GrnTTCBC: 2 air dried slides and 0.1 mL (Absolute minimum: 30 µL) of either heparinized or EDTA whole blood. Heparin preferred for some species (e.g. Crows, Jays)Please call our Client Services for information about minimums on short samples.

Reported Time See Comment

Total ProteinAlbuminGlobulinA/G RatioPEP (Protein Electrophoresis)

AST (Aspartate Transaminase)CK (Creatine Kinase)CholesterolUric AcidBile Acids

Collection Notes One m-GrnTT can be used, volume allowing. Submit m-GrnTT without centrifugation in order for HCT and WBC to be done on same tube. Prepare Slides at clinic.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistries and CBC reported out same day. PEP run Monday and Thursday with results out same day.If a microtainer (m-GrnTT or m-LTT) with whole blood is not submitted, WBC is estimated from the blood smear

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3010 Avian/Nonmammalian 13 Chemistry w/ CBC, Follow-up

CBCGlucoseCalciumPhosphorusCa/Phos Ratio

Specimen Requirements Chemistry: 0.3 mL (minimum 150 µL) heparinized plasma or 350 µL Whole Blood, m-GrnTTCBC: 2 air dried slides and 0.1 mL (Absolute minimum: 30 µL) of either heparinized or EDTA whole blood.Heparin preferred for some species (e.g. Crows, Jays)Please call our Client Services for information about minimums on short samples.

Reported Time Same Day

Total ProteinAlbuminGlobulinA/G RatioBile Acids

AST (Aspartate Transaminase)CK (Creatine Kinase)CholesterolUric Acid

Collection Notes One m-GrnTT can be used, volume allowing. Submit m-GrnTT without centrifugation in order for HCT and WBC to be done on same tube. Prepare Slides at clinic.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: If a microtainer (m-GrnTT or m-LTT) with whole blood is not submitted, WBC is estimated from the blood smear.

Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

3008 Avian/Nonmammalian 18 Chemistry Panel

Methodology: Spectrophotometry

GlucoseSodiumPotassiumSodium/Potassium RatioChloride, CalciumPhosphorus

Specimen Requirements 0.3 mL heparinized Plasma( Minimum: 0.2 mL) or 450 µL Whole Blood, m-GrnTTPlease call our Client Services for information about minimums on short samples.

Reported Time Same Day

AST (Aspartate Transaminase)CholesterolCK (Creatine Kinase)Uric Acid Bile Acids

Ca/Phos RatioTotal ProteinAlbuminGlobulinA/G RatioGGT (Gamma-glutamyltransferase)

Collection Notes None

Additional Comments: None

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3009 Avian/Nonmammalian 18 Chemistry w/ CBC

CBCGlucoseSodiumPotassiumSodium/Potassium RatioChloride, CalciumPhosphorus

Specimen Requirements Chemistry: 0.3 mL (minimum: 0.2 mL) heparinized plasma or 450 µL Whole Blood, m-GrnTTCBC: 2 air dried slides and 0.1 mL (Absolute minimum: 30 µL) of either heparinized or EDTA whole blood. Heparin preferred for some species (e.g. Crows, Jays)Please call our Client Services for information about minimums on short samples.

Reported Time Same Day

AST (Aspartate Transaminase)CholesterolCK (Creatine Kinase)Uric AcidBile Acids

Ca/Phos RatioTotal ProteinAlbuminGlobulinA/G RatioGGT (Gamma-glutamyltransferase)

Collection Notes One m-GrnTT can be used, volume allowing. Submit m-GrnTT without centrifugation in order for HCT and WBC to be done on same tube. Prepare Slides at clinic.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: If a microtainer (m-GrnTT or m-LTT) with whole blood is not submitted, WBC is estimated from the blood smear

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3012 Avian/Nonmammalian 18 Chemistry w/ CBC and Protein Electrophoresis

CBCPEP (Protein Electrophoresis)GlucoseSodiumPotassiumSodium/Potassium RatioChloride, CalciumPhosphorus

Specimen Requirements Chemistry: 0.3 mL (minimum: 0.25 mL) heparinized plasma or 550 µL Whole Blood, m-GrnTTCBC: 2 air dried slides and 0.1 mL (Absolute minimum: 30 µL) of either heparinized or EDTA whole blood.Heparin preferred for some species (e.g. Crows, Jays) Please call our Client Services for information about minimums on short samples.

Reported Time See comment

AST (Aspartate Transaminase)CholesterolCK (Creatine Kinase)Uric AcidBile AcidsPEP (Protein Electrophoresis)

Ca/Phos RatioTotal ProteinAlbuminGlobulinA/G RatioGGT (Gamma-glutamyltransferase)

Collection Notes One m-GrnTT can be used, volume allowing. Submit m-GrnTT without centrifugation in order for HCT and WBC to be done on same tube. Prepare Slides at clinic.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistries and CBC reported out same day. PEP run Monday and Thursday with results out same day. If a microtainer (m-GrnTT or m-LTT) with whole blood is not submitted, WBC is estimated from the blood smear

3013 Avian/Nonmammalian 21 Chemistry

Methodology: Spectrophotometry

GlucoseBUN (Blood Urea Nitrogen)SodiumPotassiumSodium/Potassium RatioChlorideCalcium

Specimen Requirements 0.3 mL heparinized plasma (Minimum: 0.25 mL) or 550 µL Whole Blood, m-GrnTT Please call our Client Services for information about minimums on short

Reported Time Same Day

AST (Aspartate Transaminase)CholesterolTriglyceridesCK (Creatine Kinase)Uric AcidAmylaseBile Acids

PhosphorusCa/Phos RatioTotal ProteinAlbuminGlobulinA/G RatioGGT (Gamma-glutamyltransferase)

Collection Notes None

Additional Comments: None

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3015 Avian/Nonmammalian 21-Chemistry w/ CBC

CBCGlucoseBUN (Blood Urea Nitrogen)SodiumPotassiumSodium/Potassium RatioChlorideCalcium

Specimen Requirements Chemistry: 0.3 mL heparinized plasma (minimum 250 µL) or 550 µL of whole blood.CBC: 2 air dried slides and 0.1 mL of either heparinized or EDTA whole blood. Heparin preferred for some species (e.g. Crows, Jays)Please call our Client Services for information about minimums on short samples.

Reported Time Same Day

AST (Aspartate Transaminase)CholesterolTriglyceridesCK (Creatine Kinase)Uric AcidAmylaseBile Acids

PhosphorusCa/Phos RatioTotal ProteinAlbuminGlobulinA/G RatioGGT (Gamma-glutamyltransferase)

Collection Notes One m-GrnTT can be used, volume allowing. Submit m-GrnTT without centrifugation in order for HCT and WBC to be done on same tube. Prepare Slides at clinic.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: If a microtainer (m-GrnTT or m-LTT) with whole blood is not submitted, WBC is estimated from the blood smear and PCV will be cancelled.

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3016 Avian/Nonmammalian 21-Chemistry w/ CBC and PEP

CBCGlucoseBUN (Blood Urea Nitrogen)SodiumPotassiumSodium/Potassium RatioChlorideCalcium

Specimen Requirements Chemistry: 0.3 mL (minimum: 0.27 mL) heparinized plasma or 550 µL Whole BloodCBC: 2 air dried slides and 0.1 mL (Absolute minimum: 30 µL) of either heparinized or EDTA whole blood.Heparin preferred for some species (e.g. Crows, Jays)Please call our Client Services for information about minimums on short samples.

Reported Time See comment

AST (Aspartate Transaminase)CholesterolTriglyceridesCK (Creatine Kinase)Uric AcidAmylaseBile AcidsPEP (Protein Electrophoresis)

PhosphorusCa/Phos RatioTotal ProteinAlbuminGlobulinA/G RatioGGT (Gamma-glutamyltransferase)

Collection Notes One m-GrnTT can be used, volume allowing. Submit m-GrnTT without centrifugation in order for HCT and WBC to be done on same tube. Prepare Slides at clinic.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistries and CBC reported out same day. PEP run Monday and Thursday with results out same day. If a microtainer (m-GrnTT or m-LTT) with whole blood is not submitted, WBC is estimated from the blood smear

36 Avian/Reptile CBC

Methodology: Manual

Specimen Requirements Air dried Peripheral Blood smears and 0.25 mL whole blood, m-LTT/m-GrnTT. (Minimum: 30 µL whole blood)Heparin preferred for some species (e.g. Crows, Jays)

Reported Time Same Day

Collection Notes It is always best to submit well-prepared, fresh, air-dried peripheral blood smears with your blood sample to prevent cell lysis and clumping. Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: If a microtainer is submitted, a WBC count and estimate from the slide made at the lab will be done. If only peripheral blood smears were submitted, a WBC estimate will be done from review of the peripheral blood smears.

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1036 Avian/Reptile CBC, Follow-Up

Methodology: Manual

Specimen Requirements Air dried Peripheral Blood smears and 0.25 mL whole blood, m-LTT/m-GrnTT. (Minimum: 30 µL whole blood)Heparin preferred for some species (e.g. Crows, Jays)

Reported Time Same Day

Collection Notes It is always best to submit well-prepared, fresh, air-dried peripheral blood smears with your blood sample to prevent cell lysis and cell clumping.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: If a microtainer is submitted, a WBC count and estimate from the slide made here will be done, if you send peripheral blood smears, a WBC estimate will be done from review of the peripheral blood smears. The WBC count that looks most accurate will be reported.Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

1530 Avian/Reptile, Giardia/Crypto w/ Modified Acid Fast

Giardia/Cryptosporidium (IFA)

Specimen Requirements 3-5 grams Fresh Feces, Fecal Tube

Reported Time Daily AM - Same Day

Modified Acid Fast Stain

Collection Notes None

Additional Comments: Screening for Giardia and Cryptosporidium in avian and non-mammalian species.To rule out Giardia and Cryptosporidia in mammalian species, see Giardia/Cryptosporidia IFA #240

21 Basic Canine Chemistry

Methodology: Spectrophotometry

Chemistry ScreenCK (Creatine Kinase)

Specimen Requirements 0.5 mL Serum, SST/RTT, 1.0 mL GTT

Reported Time Same Day

Amylase Lipase

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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57 Basic Feline

CBCChemistry Screen

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time See Comment

CK (Creatine Kinase)Total T4

FeLV (ELISA)FIV (IFA)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistries, CBC and FELV done same day.IFA testing done next day.In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

92 Basic Health Profile

CBCUrinalysis

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smears; Urinalysis: 5 mL fresh urine

Reported Time Same Day

Chemistry Screen Total T4

Collection Notes Please indicate method of urine collection.Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Submit sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: None

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46 Bile Acids Panel

Methodology: Spectrophotometry

Bile acids, Pre

Specimen Requirements Pre and Post required, 0.3 mL (minimum 0.1 mL) serum each, SST/RTT.

Reported Time Same Day

Bile acids, Post

Collection Notes Pre Sample: 12 hour fasting samplePost Sample: 2 hour post prandial Use a standard meal such as maintenance or puppy formula food. Minimum recommendations = Feeding 2 tsp <10 lbs, 2 tbsp >10 lbs of canned, high quality food.PLEASE NOTE: The animal should be off Actigall (Ursodiol) 48 hours prior to running a bile acid study.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Post prandial values in normal dogs/cats do not usually exceed 20 umol/L. Bile acids testing is helpful in assessing liver function.GI motility abnormalities such as vomiting or diarrhea may affect results. If bilirubin is elevated then Bile acids are not necessary; they will be increased as well.

104 Bile Acids, Single Sample

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum SST/RTT; Can test either Pre Sample (12 hour fasted sample) or Post sample (2 hour post prandial)

Reported Time Same Day

Collection Notes Use a standard meal such as maintenance or puppy formula food. Minimum recommendations = Feeding 2 tsp <10 lbs, 2 tbsp >10 lbs of canned, high quality food.Please Note: Animal should be off Actigall (Ursodiol 48 hours prior to running bile acids study.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This is a single sample evaluation, Pre OR Post sample. If BOTH Pre and Post are needed, order Bile Acids Panel 46. GI motility abnormalities such as vomiting or diarrhea may affect results. If bilirubin is elevated then Bile acids are not necessary; they will be increased as well.

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106 Bilirubin, Direct

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Can be added on to chemistry at no charge (within 7 days).

135 Bilirubin, Indirect

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

105 Bilirubin, Total

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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610 Blood Typing, Canine

Methodology: RIM

Specimen Requirements 0.5 mL Whole Blood, LTT

Reported Time Daily AM - Same Day

Collection Notes None

Additional Comments: Types for DEA 1 only. Call the Lab for universal blood donor information.

611 Blood Typing, Feline

Methodology: Agglutination

Specimen Requirements 0.5 mL Whole Blood, LTT

Reported Time Daily AM - Same Day

Collection Notes None

Additional Comments: Test consists of two antigens: A and B. Test cannot be performed on samples that exhibit auto-agglutination. Cats with type B erythrocytes will suffer an immediate and severe anaphylactic reaction when transfused with type A blood.

2144 Body Cavity Fluid Triglyceride

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Body Cavity Fluid, RTT/CTT

Reported Time Same Day

Collection Notes None

Additional Comments: Fluid triglyceride concentrations that are >100 mg/dL or are at least 2x greater than serum triglyceride concentrations support the diagnosis of a chylous effusion.

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14 Body Cavity/Joint Fluid Cytology

Methodology: Cytology

Specimen Requirements 1.0 mL Body Cavity or Joint Fluid, LTT required, RTT/CTT recommended for possible culture. Air dried slides also recommended.Smaller volumes may be sufficient for a count for joints.

Reported Time Reviewed M-Sat on Days

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Please include clinical history with sample. Descriptive report contains nucleated cell count, specific gravity, total protein, and cytology. For cytology on fluids from other sources (i.e. masses) order Cytology 318

3014 Body Cavity/Joint Fluid Cytology, Additional Source

Methodology: Cytology

Specimen Requirements 1.0 mL Body Cavity or Joint Fluid, LTT required, RTT/CTT recommended for possible culture. Air dried slides also desired.Smaller volumes may be sufficient for a count for joints.

Reported Time Reviewed M-Sat on Days

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Please include clinical history with sample.Descriptive report contains nucleated cell count, specific gravity, total protein, and cytology. For cytology on fluids from other sources (i.e. masses) please order Cytology 318

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304 Bone Marrow Cytology

Methodology: Cytology

Specimen Requirements 4 - 6 air dried, unstained thin smears of bone marrow.

Reported Time Reviewed M-Sat on Days

Collection Notes Please THOROUGHLY DRY slides before closing transport container and do not refrigerate.Protocol:1. Any bone which contains red marrow can be used as a site for aspiration or core biopsy. For small animals, collection sites include proximal femur, humerus, and iliac crest. For adult large animals, collection sites include the sternum, ribs and ilium (tuber coxae).2. The bone marrow aspiration site should be aseptically prepared and a local anesthetic given.3. Using a bone marrow biopsy needle and stylet, go through the cortical bone usually 0.5 - 1 cm deep.4. Remove the stylet and gently aspirate the specimen. As soon as marrow fills the hub of the syringe, release the plunger and remove the syringe. Collect 2-4 drops of marrow (0.2 mL -0.25 mL).ASPIRATION OF A LARGER VOLUME USUALLY RESULTS IN EXCESSIVE CONTAMINATION OF THE MARROW WITH PERIPHERAL BLOOD.5. Marrow material is expelled from the syringe onto a glass slide held at an angle of 45 degrees. Spicules will tend to stick to the slide and contaminating blood will run off.6. Smears are prepared by gentle squash technique. Slides are pulled apart quickly lengthwise from each other. As for FNA from solid tissue, let the weight of the top slide spread the bone spicules. DO NOT PRESS SLIDES TOGETHER. ALLOW SLIDES TO AIR DRY. Using this technique, make at least 4 slides. Do not refrigerate the marrow slides.7. Any remaining material in the syringe as well as what remains in the needle should be expelled into a small EDTA tube.

Additional Comments: EVALUATION OF A CBC COLLECTED ON THE SAME DAY AS THE MARROW ASPIRATE IS ABSOLUTELY ESSENTIAL FOR AN ACCURATE INTERPRETATION OF THE MARROW FINDINGS.

To include a CBC, order Bone Marrow and CBC 364;and see test for CBC requirements.

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364 Bone Marrow Cytology W/ CBC

Methodology: Cytology

Bone Marrow Cytology

Specimen Requirements Cytology: 4 - 6 air dried, unstained thin smears of bone marrowCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Reviewed M-Sat on Days

CBC

Collection Notes Please THOROUGHLY DRY slides before closing transport container and do not refrigerate.Protocol:1. Any bone which contains red marrow can be used as a site for aspiration or core biopsy. For small animals, collection sites include proximal femur, humerus, and iliac crest. For adult large animals, collection sites include the sternum, ribs and ilium (tuber coxae).2. The bone marrow aspiration site should be aseptically prepared and a local anesthetic given.3. Using a bone marrow biopsy needle and stylet, go through the cortical bone usually 0.5 - 1 cm deep.4. Remove the stylet and gently aspirate the specimen. As soon as marrow fills the hub of the syringe, release the plunger and remove the syringe. Collect 2-4 drops of marrow (0.2 mL -0.25 mL). ASPIRATION OF A LARGER VOLUME USUALLY RESULTS IN EXCESSIVE CONTAMINATION OF THE MARROW WITH PERIPHERAL BLOOD.5. Marrow material is expelled from the syringe onto a glass slide held at an angle of 45 degrees. Spicules will tend to stick to the slide and contaminating blood will run off.6. Smears are prepared by gentle squash technique. Slides are pulled apart quickly lengthwise from each other. As for FNA from solid tissue, let the weight of the top slide spread the bone spicules. DO NOT PRESS SLIDES TOGETHER. ALLOW SLIDES TO AIR DRY. Using this technique, make at least 4 slides. Do not refrigerate the marrow slides.7. Any remaining material in the syringe as well as what remains in the needle should be expelled into a small EDTA tube.

Additional Comments: Please include a complete history.

For Bone Marrow Cytology only, refer to test code 304.

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887 Bovine Chemistry Panel

Methodology: Spectrophotometry

AlbuminTotal BilirubinBUN (Blood Urea Nitrogen)GlucoseCreatinineCalcium

Specimen Requirements 0.5 mL Serum, SST/RTT, 1.0 mL GTT

Reported Time Same Day

MagnesiumPhosphorusPotassiumSodiumTotal ProteinAlb/Glob Ratio

ChlorideCholesterolCK (Creatine Kinase)GGT (Gamma-glutamyltransferase)Globulins

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

8871 Bovine Panel for Ca Supplement

Methodology: Spectrophotometry

AlbuminCalciumChlorideAlb/Glob Ratio

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

MagnesiumPhosphorusPotassium

SodiumTotal ProteinGlobulin

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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201 Brucella Screen, Canine

Methodology: IFA

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Daily AM - Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Positive results should be confirmed by AGID. This is a send-out test to Cornell University (SO4 - Brucellosis Confirmation). Additional charges will apply. This will not be sent without client approval.

1318 Buffy Coat Cytology

Methodology: Cytology

Specimen Requirements 1.0 mL Whole Blood, LTT

Reported Time Reviewed M-Sat on Days

Collection Notes None

Additional Comments: The buffy coat sample is prepared by centrifuging a whole blood sample from an EDTA Tube (LTT). The layer between the plasma and the RBCs is called the buffy coat and is examined by the Pathologist. This is the most sensitive method to look for mast cells.

108 BUN (Blood Urea Nitrogen)

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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48 BUN and Creatinine

Methodology: Spectrophotometry

Specimen Requirements 0.3 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Test includes BUN and Creatinine only, no ratio.

110 Calcium

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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1647 Canine Breeding Panel

Progesterone

Specimen Requirements Progesterone: 0.5 mL RTTCytology: 1-3 Vaginal Smears (Slides)

Reported Time Daily AM - Same Day

Cytology, Vaginal

Collection Notes Prepare the cytology sample by GENTLY rolling the swab over the slide. This technique will deliver optimal cellular material for review. Thoroughly dry the smears. DO NOT put slides into the slides holder until completely dry and do not refrigerate. Consistent labeling of tubes and smears: "Last Name, Owner, Animal, date" is very helpful when comparing serial results.Please allow all serum samples (RTT) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.Remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Timing of Sampling in Early Proestrus: 1) Progesterone should be monitored every 2-3 days to detect the preovulatory rise. 2) Vaginal Cytology should be evaluated every 2 days until >60% cornified. Rechecking: If progesterone is 1.0 - 3.0 ng/mL, consider confirming ovulation with serum progesterone concentration after at least 3 days. At ovulation, serum progesterone ranges from 3.8 - 10 ng/mL. Optimal breeding (fresh semen) is 2 days after ovulation.

1012 Canine GI Panel (cPL/TLI/Cobalamin/Folate)

Methodology: Chemiluminescent Immunoassay/Lateral Flow

CobalaminFolate

Specimen Requirements 1.0 mL Serum SST/RTT, hemolyzed samples should NOT be submitted for Folate testing.

Reported Time Mon, Wed, Fri, Same day

cPLI TLI

Collection Notes Fast animals 12-18 hours (diabetic animals 6-8 hours) before draw.Cover samples with foil, vet wrap, or a paper towel to protected from light (folate and cobalamin can be light sensitive) It is recommended to separate serum and freeze the sample if transport to lab is to be delayed.

Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: For Felines see Feline TLI/PLI/Cobalamin/Folate 1689.

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58 Canine Heartworm Panel

Methodology: ELISA/Knotts

Heartworm Antigen

Specimen Requirements 0.5 mL Serum, SST/RTT, and 1.0 mL Whole Blood, LTT(Test can be run on only LTT, volume 2.0 mL Whole Blood)

Reported Time Same Day

Microfilaria

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Testing recommended in dogs 7 months and older. Test sensitivity is limited to detection of adult female heartworm antigen. High worm burdens can have false negative antigen results. If the result is unexpected, consider re-testing with heat treatment.Positive antigen results verified by RIM test. This test is not recommended for use in cats as they usually have a low worm load (see Feline Heartworm Panel 1629).

To run only Heartworm antigen or microfilaria see: Heartworm Antigen 238 or Microfilaria 212.

156 Canine Immunization Screen

Methodology: IFA

Canine Distemper virus

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Monday, Thursday: Same Day

Parvovirus

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: For Vaccination status. Screens for antibodies to Canine Distemper virus and Parvovirus.

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1005 Canine PLI and TLI

Methodology: Chemiluminescent Immunoassay/Lateral Flow

Specimen Requirements 0.5 mL Serum SST/RTT For cPLI: Excessive hemolysis may obscure the results and sample could be rejected.

Reported Time Same Day/Next Day

Collection Notes Serum must be fasted 12-18 hours (diabetic animals 6-8 hours) and non-hemolyzed/non-lipemic.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments:

1013 Canine PLI/Cobalamin/Folate

Methodology: Chemiluminescent Immunoassay/Lateral Flow

cPLI

Specimen Requirements 1.0 mL Serum SST/RTT, hemolyzed samples should NOT be submitted for Folate testing.

Reported Time Mon, Wed, Fri, Same day

Cobalamin Folate

Collection Notes Fast animals 12-18 hours (diabetic animals 6-8 hours) before draw.Cover samples with foil, vet wrap, or a paper towel to protected from light (folate and cobalamin can be light sensitive) It is recommended to separate serum and freeze the sample if transport to lab is to be delayed.

Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: For Felines see Feline PLI/Cobalamin/Folate 4689.

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111 Carbon Dioxide

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Total CO2 is an estimate of serum bicarbonate.

44 CBC w/ Differential

Methodology: Flow Cytometry

Specimen Requirements 0.5 mL Whole Blood, LTT, and air dried peripheral blood smears

Reported Time Same Day

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: None

34 CBC w/ Fibrinogen and Plasma Protein

Methodology: Flow Cytometry/Refractometry

Specimen Requirements 1.5 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: None

24 CBC w/ Plasma Protein

Methodology: Flow Cytometry/Refractometry

Specimen Requirements 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: None

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55 CBC, Exotic/Small Mammal

Methodology: Manual

Specimen Requirements Hematocrit and 3 air dried slides.

Reported Time Same Day

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Includes Hct, WBC estimate, and differential. Used for "short" sample specimens.

73 CBC, Follow-Up

Methodology: Flow Cytometry

Specimen Requirements 1.0 mL Whole Blood, LTT. Air dried peripheral blood smears also recommended

Reported Time Same Day

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

1044 CBC, Platelet if Indicated

Methodology: Flow Cytometry

Specimen Requirements 0.5 mL Whole Blood, LTT

Reported Time Same Day

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: It is possible a platelet count cannot be performed due to clumping.

1073 CBC, Platelet if Indicated, Follow-Up

Methodology: Flow Cytometry/Manual

Specimen Requirements 0.5 mL Whole Blood, LTT

Reported Time Same Day

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Submit within 30 days of original test. Previous Case ID is required to activate the discounted price. It is possible a platelet count cannot be performed due to clumping.

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1 Chemistry Screen

Methodology: Spectrophotometry

GlucoseBUN (Blood Urea Nitrogen)CreatinineSodiumPotassiumSodium/Potassium RatioChlorideCarbon Dioxide (CO2)

Specimen Requirements 0.5 mL Serum, SST/RTTMinimum is 250uL serum

Reported Time Same Day

Anion GapCalciumPhosphorusTotal ProteinAlbuminGlobulinA/G Ratio

Bilirubin, TotalALP (Alkaline Phosphatase)ALT (Alanine Transaminase)AST (Aspartate Transaminase)GGT (Gamma-glutamyltransferase)CholesterolCalculated Osmolality

Collection Notes Prolonged contact with cells can dramatically affect glucose valuesFor accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

853 Chemistry Screen w/ T4 and Feline Viral II

Chemistry ScreenTotal T4

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time See Comment

FeLV (ELISA)FIV (IFA)

FCoV (FIP) Screen (IFA)

Test Alias: Special Feline w/Chemistry Screen

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistry, and FeLV run same day, FIV/FIP run daily, AM. Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted. Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

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71 Chemistry Screen, Follow-up

Methodology: Spectrophotometry

GlucoseBUN (Blood Urea Nitrogen)CreatinineSodiumPotassiumSodium/Potassium RatioChloride

Specimen Requirements 0.5 mL Serum, SST/RTT, 1.0 mL GGT

Reported Time Same Day

CO2 (Carbon Dioxide)Anion GapCalciumPhosphorusTotal ProteinAlbuminGlobulinA/G Ratio

Bilirubin, TotalALP (Alkaline Phosphatase)ALT (Alanine Transaminase)AST (Aspartate Transaminase)GGT (Gamma-glutamyltransferase)CholesterolCalculated Osmolality

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

98 Chemistry Screen, Large Animal

Methodology: Spectrophotometry

GlucoseBUN (Blood Urea Nitrogen)CreatinineSodiumPotassiumSodium/Potassium RatioChlorideCO2 (Carbon Dioxide)Anion Gap

Specimen Requirements 0.5 mL Serum, SST/RTT, 1.0 mL GTT

Reported Time Same Day

Calcium PhosphorusCa/Phos RatioCalculated OsmolalityTotal ProteinAlbuminGlobulinAlbumin/GlobulinBilirubin, Total

ALP (Alkaline Phosphatase)AST (Aspartate Transaminase)GGT (Gamma-glutamyltransferase)CholesterolCK (Creatine Kinase)AmylaseLipaseBile Acids

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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112 Chloride

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

113 Cholesterol

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

116 CK (Creatine Kinase)

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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83 Coagulation Panel I

Methodology: Endpoint Clot Detection

Prothrombin Time (PT)

Specimen Requirements 1 BTT Citrated Plasma required, see collection note for volume details.

Reported Time Same Day

APTT

Collection Notes Citrated tubes (BTT) provided by Phoenix Laboratory hold 2.7 mL of blood (to the top of the label). Tubes must be filled with 2.7 mL of blood. If collecting a smaller volume of blood (<2.7mL) the blood to anticoagulant ratio should be 9:1. Please call lab with any questions.Protocol: 1. Draw fresh venous blood into BTT until full (to top of label) but not beyond the top of the tube label. No clotting should be observed in the tube. 2. Cool to 4° C (refrigeration) until analysis. 3. If a delay greater than 24 hours is expected prior to analysis, then the BTT should be spun in a centrifuge, the plasma separated and placed in a CTT or other non-additive tube to be frozen until analysis.

Additional Comments: The ratio of blood to anticoagulant is critical for valid coagulation results. Any clotting will results in the tube being rejected for analysis.

10 Coagulation Panel II

CBC w/DifferentialPlatelet Count

Specimen Requirements 1 BTT, Centrifuge and Separate plasma (0.5mL minimum) and refrigerate. 1 LTT and 2 air-dried Blood smears

Reported Time Same Day

FibrinogenAPTT

Prothrombin Time (PT)

Collection Notes Citrated tubes (BTT) provided by Phoenix Laboratory hold 2.7 mL of blood (to the top of the label). Tubes must be filled with 2.7 mL of blood. If collecting a smaller volume of blood (<2.7mL) the blood to anticoagulant ratio should be 9:1. Please call lab with any questions.Protocol: 1. Draw fresh venous blood into BTT until full (to top of label) but not beyond the top of the tube label. No clotting should be observed in the tube. 2. Cool to 4° C (refrigeration) until analysis. 3. If a delay greater than 24 hours is expected prior to analysis, then the BTT should be spun in a centrifuge, the plasma separated and placed in a CTT or other non-additive tube to be frozen until analysis.

Additional Comments: The ratio of blood to anticoagulant is critical for valid coagulation results. Any clotting will results in the tube being rejected for analysis.Fibrinogen performed by heat precipitation.

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18 Coagulation Panel III

CBCPlatelet Count

Specimen Requirements CBC and Fibrinogen: 1.5 mL LTT and air dried peripheral blood smearsCoagulation: 1 BTT, Centrifuge and separate plasma (0.5 mL minimum) and refrigerate.

Reported Time Same Day

D-DimerFibrinogen

Prothrombin Time (PT)APTT

Collection Notes Citrated tubes (BTT) provided by Phoenix Laboratory hold 2.7 mL of blood (to the top of the label). Tubes must be filled with 2.7 mL of blood. If collecting a smaller volume of blood (<2.7mL) the blood to anticoagulant ratio should be 9:1. Please call lab with any questions.Protocol: 1. Draw fresh venous blood into BTT until full (to top of label) but not beyond the top of the tube label. No clotting should be observed in the tube. 2. Cool to 4° C (refrigeration) until analysis. 3. If a delay greater than 24 hours is expected prior to analysis, then the BTT should be spun in a centrifuge, the plasma separated and placed in a CTT or other non-additive tube to be frozen until analysis.

Additional Comments: The ratio of blood to anticoagulant is critical for valid coagulation results. Any clotting will results in the tube being rejected for analysis.Fibrinogen performed by heat precipitation.

70 Cobalamin (Vitamin B12)

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum, SST/RTT.

Reported Time Mon/Wed/Fri

Collection Notes Fast animals 12-18 hours (diabetic animals 6-8 hours) before draw.Cover sample with foil, vet wrap, or a paper towel to protected from light. sensitive).It is recommended to separate serum and freeze the sample if transport to lab is to be delayed.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Cobalamin is run in-house Monday, Wednesday, and Friday; reported out same day. Cobalamin results >1000 are re-run on dilution and reported out.

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2689 Cobalamin and Folate

Methodology: Chemiluminescent Immunoassay

Specimen Requirements Serum must be fasted 12-18 hours (diabetic animals 6-8 hours). Sample must be non-hemolyzed for folate testing and avoid lipemic samples. Cover samples with foil, vet wrap, or a paper towel to protected from light (folate and cobalamin are light sensitive)

Reported Time Mon/Wed/Fri

Collection Notes Fast animals 12-18 hours (diabetic animals 6-8 hours) before draw.

Additional Comments: Cobalamin and Folate are run in-house Monday, Wednesday, and Friday; reported out same day. Folates >24.0 ug/dL and cobalamin >1000 are re-run on dilution and reported.

614 Coggins EIA, AGID

Methodology: AGID

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Up to 36 hours

Collection Notes The animal's name MUST be on the tube and MUST match the name listed on the form. Veterinarian signature and animal location is also REQUIRED.

Please allow sample to clot (up to 20 minutes) and then spin down sample. If using a RTT, remove serum and put into a fresh RTT/CTT. Avoid lipemia and hemolysis.

Additional Comments: USDA Equine Anemia Special Form or Global Vet Link Form required. Please print clearly as illegible documents can cause delay. Veterinarian signature and animal location are REQUIRED. Blood tube MUST be labeled with the animal's name that is listed on the accompanying form. If any of these are missing it can cause a delay or return of sample and/or form for corrections (additional charges may apply).

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601 Coggins, ELISA

Methodology: ELISA

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes The animal's name MUST be on the tube and MUST match the name listed on the form. Veterinarian signature and animal location is also REQUIRED.

Please allow sample to clot (up to 20 minutes) and then spin down sample. If using a RTT, remove serum and put into a fresh RTT/CTT. Avoid lipemia and hemolysis.

Additional Comments: USDA Equine Anemia Special Form or Global Vet Link Form required. Please print clearly, as illegible documents can cause delay. Veterinarian signature and animal location are REQUIRED. Blood tube MUST be labeled with the animal's name that is listed on the accompanying form. If any of these are missing it can cause a delay or return of sample and/or form for corrections (additional charges may apply).

362 Coombs

Methodology: Direct Antiglobulin

Specimen Requirements 1.0 mL Whole Blood, LTT

Reported Time Daily, AM

Collection Notes Corticosteroids can cause false negative Coombs results if >7 days of therapy, therefore consider discontinuing corticosteroid use 2-3 weeks prior to testing.

Additional Comments: Steroids will affect this test result, however, studies have shown that corticosteroid therapy of <7 days do not cause false negatives.

365 Coombs,  Add-On

Methodology: Direct Antiglobulin

Specimen Requirements 1.0 mL Whole Blood, LTT

Reported Time Daily, AM

Collection Notes Corticosteroids can cause false negative Coombs results if >7 days of therapy, therefore consider discontinuing corticosteroid use 2-3 weeks prior to testing.

Additional Comments: Steroids will affect this test result, however, studies have shown that corticosteroid therapy of <7 days do not cause false negatives..

Available as an add-on to an existing panel (within 7 days). Previous case ID is required to activate the discounted price.

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2231 Cortisol, Resting

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum, RTT

Reported Time Same Day

Collection Notes If the patient has been on steroids for >14 days, patient should be taperdown and discontinue for at least 2 weeks prior to testing. If <14 days, discontinue for 38 hours prior to testing.DO NOT USE SST TO COLLECT OR SPIN DOWN SAMPLE

Additional Comments: A patient with a resting cortisol >2.0 ug/dL has a 99% chance of NOT having Addison's disease.With a resting cortisol of <2.0 ug/dL, an ACTH Response 39 is needed to rule-in Addison's disease. This interpretation is valid if no oral or topical steroids have been given in the last 6 weeks. A resting cortisol cannot be used to rule- in or out hyperadrenocorticism. A LDDS 38 and/or ACTH Response 39 needs to be performed.

233 Cortisol, Urine

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Urine, Urine Tube/CTTIf sample is bloody or very cloudy/cellular, submit a larger volume (0.5 mL minimum) as the sample will need to be centrifuged.

Reported Time Same Day

Collection Notes None

Additional Comments: None

1000 cPL

Methodology: Lateral Flow

Specimen Requirements 0.25 mL Serum SST/RTT. Excessive hemolysis may obscure the results and sample could be rejected.

Reported Time Same Day AM/PM

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis. EDTA, sodium and lithium heparin sample tubes are acceptable.

Additional Comments: Semi-quantitative Canine Pancreatic Lipase Test. This test is specific for the determination of pancreas-specific lipase levels in canine serum or plasma.

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115 Creatinine

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

27 Crossmatch Complete

Methodology: Tube Agglutination

Crossmatch Major

Specimen Requirements 1.0 mL Serum, RTT and Whole Blood, LTT required for Recipient and for each donor tested. **Samples can not be hemolyzed.**

Reported Time Same Day

Crossmatch Minor

Collection Notes None

Additional Comments: Major: Determines if donor's RBCs are compatible with the recipient's serum.Minor: Determines if donor's plasma is compatible with the recipient's RBCs.A crossmatch is used prior to transfusion to determine if the blood to be transfused is compatible with the animal that will be receiving the transfusion. Individual charge for each tested donor. For Canine or feline only.

For only Major or Minor testing, see Crossmatch, Major 314, or Crossmatch, Minor 313.

314 Crossmatch, Major

Methodology: Tube Agglutination

Specimen Requirements Donor: 1.0 mL Whole Blood, LTT; Recipient: 0.5 mL Serum, RTTBoth samples need to be free of hemolysis

Reported Time Daily, AM

Collection Notes None

Additional Comments: Determines if donor's RBCs and recipient's serum are compatible.

For complete panel, refer to Crossmatch Complete (Major and Minor) test 27. Test will be cancelled if hemolysis is present.

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313 Crossmatch, Minor

Methdology: Tube Agglutination

Specimen Requirements Donor: 0.5 mL Serum, RTT; Recipient: 1.0 mL Whole Blood, LTTBoth samples need to be free of hemolysis

Reported Time Daily, AM

Collection Notes None

Additional Comments: Determines if donor's serum and recipient's RBCs are compatible.

For complete panel, refer to Crossmatch Complete (Major and Minor) test 27. Test will be cancelled if hemolysis is present.

29 CSF Analysis

Methodology: Cytology/Spectrophotometry

Specimen Requirements 0.5 mL CSF LTT and RTT/CTT, Minimum 0.2mL in each tube.

Reported Time Reviewed M-Sat on Days

Collection Notes Protocol: 1. To minimize blood contamination, it is recommended to allow the CSF to drip into a small EDTA tube. 2. A CTT is recommended for chemistries and culture.

Additional Comments: All CSF specimens receive nucleated cell count, RBC count, glucose, microprotein, and cytology. We request 1 mL total, but will work with any volume. If only a small amount of CSF is collected (<0.5 mL), please prioritize desired testing. EDTA (LTT) inhibits bacterial growth and can not be used for culture.

1029 CSF Analysis, Additional Site

Methodology: Cytology/Spectrophotometry

Specimen Requirements 0.5 mL CSF LTT and CTT, Minimum 0.2mL in each tube.

Reported Time Reviewed M-Sat on Days

Collection Notes Protocol: 1. To minimize blood contamination, it is recommended to allow the CSF to drip into a small EDTA tube.2. A CTT is recommended for chemistries and culture.

Additional Comments: All CSF specimens receive nucleated cell count, RBC count, glucose, microprotein, an cytology. We request 1 mL total, but will work with any volume. If only a small amount of CSF is collected <0.5 mL, please prioritize testing desired. EDTA (LTT) inhibits bacterial growth and can not be used for culture.

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51 CSF Panel, Special

CSF Analysis

Specimen Requirements 0.5 mL each LTT and/or RTT/CTT. Minimum: 0.4 mL total

Reported Time Reviewed M-Sat on days

CSF CK (Creatine Kinase) Pandy's Test

Collection Notes Protocol: 1. To minimize blood contamination, it is recommended to allow the CSF to drip into a small EDTA tube. 2. A CTT is recommended for chemistries and culture.

Additional Comments: Includes CSF panel 29, as well as CK value and Pandy's test. We request 1 mL total, but will work with any volume.If only a small amount of CSF is collected (<0.5 mL), please prioritize desired tests. EDTA (LTT) inhibits bacterial growth and cannot be used for culture.

505 Culture & Sensitivity, Aerobic

Methodology: Culture

Specimen Requirements Bacterial culture swab with transport medium or fluid/tissue in CTT or sterile tube

Reported Time 2-5 Days

Collection Notes Please indicate culture site and animal species for proper processing. If submitting tissue, moisten with small amount of sterile saline (free of preservative) if piece of tissue is small enough to dehydrate en route to laboratory.

Additional Comments: Sensitivity and gram stain included. Do not submit samples in lavender top tube; EDTA is bacteriostatic.

516 Culture & Sensitivity, Milk

Methodology: Culture

Specimen Requirements 1 mL milk in CTT or sterile tube.

Reported Time 2-5 days

Collection Notes None

Additional Comments: Sensitivity and gram stain included. Do not submit samples in lavender top tube; EDTA is bacteriostatic.

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524 Culture ID/Sensitivity if Indicated, Avian

Methodology: Culture

Specimen Requirements Bacterial culture swab with transport medium or fluid/tissue in CTT or sterile tube

Reported Time 2-5 Days

Collection Notes None

Additional Comments: For the detection of gram negative rods in psittacines (parrots). Sensitivities will be performed on each isolate for an additional charge per isolate.

512 Culture, Aerobic ID Only

Methodology: Culture

Specimen Requirements Bacterial culture swab with transport medium or fluid/tissue in CTT or sterile tube

Reported Time 2-5 Days

Collection Notes Please indicate culture site and animal species for proper processing. If submitting tissue, moisten with small amount of sterile saline (free of preservative) if piece of tissue is small enough to dehydrate en route to laboratory.

Additional Comments: Sensitivity and gram stain included. Do not submit samples in lavender top tube; EDTA is bacteriostatic.

514 Culture, Aerobic ID only, Anaerobic  ID only

Methodology: Culture

Specimen Requirements Bacterial culture swab with transport medium or fresh tissue in sterile CTT.

Reported Time 2-5 Days

Collection Notes Please indicate culture site and animal species for proper processing. If submitting tissue, moisten with small amount of sterile saline (free of preservative) if piece of tissue is small enough to dehydrate en route to laboratory.

Additional Comments: None

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500 Culture, Aerobic w/ Sensitivity/Anaerobic Culture, ID only

Methodology: Culture

Specimen Requirements Bacterial culture swab with transport medium or fresh tissue in sterile CTT

Reported Time 2-5 Days

Collection Notes Please indicate culture site and animal species for proper processing. If submitting tissue, moisten with small amount of sterile saline (free of preservative) if piece of tissue is small enough to dehydrate en route to laboratory.

Additional Comments: Aerobic culture includes sensitivities on up to 2 isolates. Anaerobic sensitivities are not available. Includes gram stain. Do not submit samples in lavender top tube; EDTA is bacteriostatic.

502 Culture, Anaerobic (ID Only)

Methodology: Culture

Specimen Requirements Bacterial culture swab with transport medium or fluid/tissue in CTT or sterile tube

Reported Time 2-5 Days

Collection Notes Please indicate culture site and animal species for proper processing. If submitting tissue, moisten with small amount of sterile saline (free of preservative) if piece of tissue is small enough to dehydrate en route to laboratory.

Additional Comments: Anaerobic sensitivities are not available. Do not submit samples in lavender top tube; EDTA is bacteriostatic.

503 Culture, Blood

Methodology: Culture

Specimen Requirements Two aerobic blood culture bottles should be submitted (available upon request). Blood should be drawn from two sites (i.e. left jugular, right saphenous)

Reported Time 24 hr preliminary report, Final at 7 days

Collection Notes 1. Label each blood culture bottle with the date, time, and draw site. Blood should be drawn from two sites (i.e. left jugular, right saphenous), at least one hour apart.2. Thoroughly disinfect blood draw sites (shave if needed). Inoculate the bottles with up to 3mL of blood. NOTE: If less than 3mL is used for inoculation, it may take longer for the blood culture to be detected as positive. 3. After inoculation, the bottles can be incubated at 37 degrees Celsius, or left at room temperature until pick up. DO NOT REFRIGERATE.

Additional Comments: Culture includes susceptibility panels for 2 pathogens. Bottles are incubated for 7 days and subcultured at regular intervals.

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507 Culture, Dermatology

Methodology: Culture

Specimen Requirements Bacterial culture swab with transport medium or fluid/tissue in CTT or sterile tube

Reported Time 2-5 Days

Collection Notes None

Additional Comments: Sensitivity and gram stain included. Do not submit samples in lavender top tube; EDTA is bacteriostatic.Includes standard sensitivity profile plus topical medications (neomycin, tobramycin, polymyxin B, ciprofloxacin, and orbifloxacin). Up to two isolates are included.

508 Culture, Equine Uterine

Methodology: Culture

Specimen Requirements Bacterial culture swab with transport medium

Reported Time 2-5 Days

Collection Notes None

Additional Comments: Normal test value is "No Beta-hemolytic streptococci isolated."

Refer to test #862 Equine Uterine Screen for combo test with uterine cytology.

519 Culture, Feces

Methodology: Culture

Specimen Requirements 5 grams of Fresh Feces, Fecal Tube or bag.

Reported Time 36 - 72 hours

Collection Notes None

Additional Comments: Fecal culture for the detection of Salmonella and Campylobacter. Shigella and Yersinia can be ruled out upon request. Additional charges may apply.

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504 Culture, Fungal

Methodology: Culture

Specimen Requirements Hair or nail clippings in CTT, skin scrapings in CTT, bacterial culture swab with transport medium, fluid in CTT or sterile tube, or fresh tissue in CTT or sterile tube accepted.

Reported Time Preliminary reported at 1 week. Cultures are held for up to 4 weeks.

Collection Notes Please include culture site and animal species for proper processing. If submitting tissue, moisten with small amount of sterile saline (free of preservative) if piece of tissue is small enough to dehydrate en route to laboratory.

Additional Comments: Updates reported weekly on Sunday.

618 Culture, Mycoplasma

Methodology: Culture

Specimen Requirements Swab in Mycoplasma Transport Media, exudates from: eye, nostril, trachea, synovial fluid and/or genitals or fluid in CTT or sterile tube.

Reported Time Preliminary report at 1 week. Finalized after 2 weeks.

Collection Notes Sample collection should be made with synthetic swabs. Cotton tips and wooden stick swabs inhibit growth of Mycoplasma. Media should be frozen or refrigerated before and after collection. Transport to the laboratory as soon as possible.

Additional Comments: Mycoplasma sensitivities are not available. To rule out Ureaplasma, See our Sendout catalog, Ureaplasma Culture SO111.

506 Culture, Urine

Methodology: Culture

Specimen Requirements 0.5 mL Urine, Urine Tube/CTT.

Reported Time 36 - 72 hours, No Growth cultures finalized after 48 hours.

Collection Notes Whenever possible, urine should be collected by cystocentesis. If not possible, collect catheterized or free catch urine sample into sterile container. Refrigerate urine sample after collection.

Additional Comments: Colony counts are not possible if a plate, broth, or swab is submitted. Culture includes sensitivities on up to 2 isolates. Cultures are quantitated and reported in CFU/mL. No growth cultures are finalized after 48 hours of incubation.

To include with a urinalysis, refer to Urinalysis with Culture 548.To add on to previous urine test, refer to Urine Culture Add-on 549.

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318 Cytology

Methodology: Cytology

Specimen Requirements 3-5 air dried slides or tissue imprints, unstained.Remaining fluid may be submitted as well in LTT and/or CTT at no additional cost. For cytology on trans/endotracheal wash, fluid in LTT and CTT recommended.

Reported Time Reviewed M-Sat on Days

Collection Notes FINE NEEDLE ASPIRATE PROTOCOL:1. If the cytology is a fine needle aspirate, use a 21g. -22 g. needle (smaller needles increase cell rupture) and 3-12 mL syringe depending on material/tissue aspirated. The softer the tissue, the smaller the syringe and needle necessary for aspiration. NOTE: Lymph nodes often do not require suction as this increases chance of rupture.2. Insert the needle into the mass (avoid the center of larger lesions as they are apt to be necrotic). In order to get the most representative sample the needle should be directed in at least 3 different directions with suction applied.3. Release the negative pressure slowly and withdraw the needle from the mass.4. Remove the needle,from syringe, fill the syringe with air, replace the needle and force the cellular material onto a slide or slides.5. Cover the material on the slide with another slide, allow the material to spread and then horizontally pull the slides apart without pressure. This must be done quickly to avoid clot formation.6. Quickly air dry the slides. DO NOT HEAT FIX. Try to make 3 - 5 slides from each lesion. Do not package the slides for transport until thoroughly dried. Do not refrigerate slides.

TISSUE IMPRESSION PROTOCOL:1. Impression smears can be made from an excised mass using the TOUCH IMPRINT TECHNIQUE. Blot tissue onto paper towel, then onto slides.2. Quickly air dry the slides. DO NOT HEAT FIX.3. Do not package the slides for transport until thoroughly dried. Do not refrigerate slides. Note that impression smears of ulcerated, dermal lesions are often of low diagnostic utility. VAGINAL CYTOLOGY FOR ESTRUS PROTOCOL:1. Insert the cotton tip swab against the caudodorsal surface of the vaginal vault (avoid the vulva and clitoral fossa) and advance the swab in a craniodorsal direction until it passes over the ischial arch. The swab should reach the pelvic canal.2. Rotate the swab 360 degrees in each direction and withdraw.3. For optimal exfoliation, gently roll the swab across the surface of slide, DO NOT SMEAR.4. Dry thoroughly before placing in slide container. Do not refrigerate slides.

Additional Comments: Complete history and location of lesion are essential for accurate results. Multiple lymph nodes are charged as one site. If the cytology is a paracentesis (abdominal, paracardial, or thoracic) or joint fluid with submission of fluid, refer to test Fluid Cytology (Body Cavity/ Joint Fluid) 14. Note that impression smears of ulcerated, dermal lesions are often of low diagnostic utility. EDTA (LTT) inhibits bacterial growth. If culture is required, use plain CTT or sterile transport tube.

Test includes microscopic description, diagnosis, and comments.Price is for a single site. Every site after is an additional $26.10.

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421 Cytology Slide Prep Only

Specimen Requirements Requires fluid or other cytology sample, LTT/CTT

Reported Time Varies

Collection Notes None

Additional Comments: This charge is for preparing cytology slides from samples submitted only. Slides will be sent on to destination provided (additional shipping charges may apply).To have a cytology performed, order Cytology 318.

323 D-Dimer

Methodology: Latex Agglutination

Specimen Requirements 0.5 mL Citrated PlasmaSee Collection notes for details about proper BTT Volume.

Reported Time Daily, AM

Collection Notes Citrated tubes (BTT) provided by Phoenix Laboratory hold 2.7 mL of blood (to the top of the label). Tubes must be filled with 2.7 mL of blood. If collecting a smaller volume of blood (<2.7mL) the blood to anticoagulant ratio should be 9:1.Protocol: 1. Draw fresh venous blood into BTT until full (to top of label) but not beyond the top of the tube label. No clotting should be observed in the tube. 2. Cool to 4° C (refrigeration) until analysis. 3. If a delay greater than 24 hours is expected prior to analysis, then the BTT should be spun in a centrifuge, the plasma separated and placed in a CTT or other non-additive tube to be frozen until analysis.

Additional Comments: THE RATIO OF BLOOD TO ANTICOAGULANT IS CRITICAL FOR VALID RESULTS. See Collection Notes.

523 Dermatophilus Prep

Methodology: Microscopic Examination

Specimen Requirements Skin scrapings or skin crusts submitted in a CTT or sterile container

Reported Time Next day

Collection Notes None

Additional Comments: Dermatophilus Prep should be used as a quick screen only. If prep is negative and Dermatophilus is suspected, it is recommended to run an Aerobic Culture, ID only, test code 512, before ruling out Dermatophilus congolensis as source of infection.

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38 Dexamethasone Suppression

Methodology: Chemiluminescent Immunoassay

Pre Cortisol

Specimen Requirements 0.5 mL Serum each RTTx3; Pre, 4 hour, 8 hour post. DO NOT USE SST TO COLLECT OR SPIN DOWN SAMPLE

Reported Time Same Day

4 hr Post Cortisol 8 hr Post Cortisol

Collection Notes Collection Protocol:

Low Dose: NOTE: During the low dose dexamethasone suppression test, patient stress should be kept to a minimum. No other diagnostic procedures or grooming should be performed during the testing period.1. Collect 0.5 mL serum pre-injection. (RTT,CTT)2. Inject 0.01 mg/kg dexamethasone sodium phosphate IV.3. Collect 2 post samples at 3-4 and 8 hours post injection. (RTT,CTT) Centrifuge, separate serum into a sterile transport tube (RTT,CTT), and refrigerate or freeze until transport to the Lab.

High Dose:1. Collect 0.5 mL serum pre-injection. (RTT,CTT)2. Inject 0.1 mg/kg dexamethasone sodium phosphate IV.3. Collect 2 post samples at 4 and 8 hours post injection. Label each tube accordingly. (RTT,CTT) Centrifuge, separate serum into a sterile transport tube (RTT,CTT), and refrigerate or freeze until transport to the Lab. DO NOT FREEZE SPECIMENS IN GLASS TUBES.

Additional Comments: PLEASE NOTE: All three samples should be submitted together. A dexamethasone suppression is done to CONFIRM clinical suspicion of hyperadrenocorticism. Stressful diseases (ill diabetics, renal failure, etc.) and current drug therapy may cause false positives. Prednisone, prednisolone, and other corticosteroids (except dexamethasone) will cross react. Long term steroid therapy will suppress adrenal function. Recommend these drugs be discontinued at least 48 hrs before testing. Extra charge for more than 3 samples - please call the Laboratory for information. Up to 40% of pituitary dependent and adrenal dependent hyperadrenocorticoid dogs do not show suppression in LDDST. These dogs require HDDST, ACTH concentrations and/or abdominal imaging for further characterization.

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2038 Dexamethasone Suppression, Equine

Methodology: Chemiluminescent Immunoassay

Cortisol Pre

Specimen Requirements Pre and Post required, 0.5 mL (minimum 0.3mL) serum each, RTT ONLY

Reported Time Same Day

Cortisol 19-hr Post

Collection Notes PROTOCOL: DO NOT USE SST TO COLLECT OR SPINDOWN SAMPLE1.  Begin test between 4-6 PM. 2.  Draw a pre-injection blood sample into a RTT and label PRE. 3.  Inject 40 ug/kg of DEXAMETHASONE intramuscularly. 4.  Nineteen hours post-injection, draw a blood sample into a RTT and label POST-19.  5. Blood samples should be centrifuged, the serum separated, placed in a clean tube, and refrigerated or frozen until submitted to the Lab. 6.  Submit both samples at one time.

Additional Comments: This test is no longer recommended as the primary test for diagnosis of Pituitary Pars Intermedia Dysfunction (PPID)/Equine Cushing's Disease.  Please see test code 697 (ACTH, plasma) for additional information on testing for PPID. Normal horses: Cortisol </= 1.0 ug/dL 19 hours post dexamethasone. Interpret with clinical signs and rule out other conditions. Also, if performing this test in the fall, starting in September, interpret a failure to suppress with caution as false positives occasionally occur at that time of the year. Prednisone, prednisolone, and other corticosteroids (except dexamethasone) will cross react. Recommend these drugs be discontinued at least 24 hrs before testing.

621 Distemper, FA

Methodology: FA

Specimen Requirements 1.0 mL Whole Blood LTT or conjunctival smears/swab or can run on both.

Reported Time Next Day

Collection Notes None

Additional Comments: May be positive for up to 3 weeks, post infection, in conjunctival smears; 2-5 days in whole blood.

Test set up next day, resulted by end of day.

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16 Electrolytes Screen

Methodology: Spectrophotometry

SodiumPotassium

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

ChlorideCarbon Dioxide (CO2)

Anion GapSodium/Potassium Ratio

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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1008 Endocrine Panel

Chemistry Screen

Specimen Requirements 3 RTTs, Label each with draw time (0, 4, and 8 hour) 0.5 mL each ( minimum 0.1 mL for 4 and 8 hr posts).DO NOT USE SST TO COLLECT OR SPIN DOWN SAMPLE

Reported Time Same Day

Dexamethasone Suppression - 3 Cortisols

Collection Notes Collection Protocol: Low Dose: NOTE: During the low dose dexamethasone suppression test, patient stress should be kept to a minimum. No other diagnostic procedures or grooming should be performed during the testing period.1. Collect 0.5 mL serum pre-injection. (RTT,CTT)2. Inject 0.01 mg/kg dexamethasone, IV.3. Collect 2 post samples at 3-4 and 8 hours post injection. (RTT,CTT) Centrifuge, separate serum into a sterile transport tube (RTT,CTT), and refrigerate or freeze until transport to the Lab.

High Dose:1. Collect 0.5 mL serum pre-injection. (RTT,CTT)2. Inject 0.1 mg/kg dexamethasone, IV.3. Collect 2 post samples at 4 and 8 hours post injection. Label each tube accordingly. (RTT,CTT) Centrifuge, separate serum into a sterile transport tube (RTT,CTT), and refrigerate or freeze until transport to the Lab. DO NOT FREEZE SPECIMENS IN GLASS TUBES.

Additional Comments: PLEASE NOTE: All three samples should be submitted together. A dexamethasone suppression is done to CONFIRM clinical suspicion of hyperadrenocorticism. Stressful diseases (ill diabetics, renal failure, etc.) and current drug therapy may cause false positives. Prednisone, prednisolone, and other corticosteroids (except dexamethasone) will cross react. Long term steroid therapy will suppress adrenal function. Recommend these drugs be discontinued at least 48 hrs before testing. Extra charge for more than 3 samples - please call the Laboratory for information. Up to 40% of pituitary dependent and adrenal dependent hyperadrenocorticoid dogs do not show suppression in LDDST. These dogs require HDDST, ACTH concentrations and/or abdominal imaging for further characterization.

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30 Equine Chemistry Screen

Methodology: Spectrophotometry

GlucoseBUN (Blood Urea Nitrogen)CreatinineSodiumPotassiumSodium/Potassium RatioChlorideCarbon Dioxide (CO2)

Specimen Requirements 1.0 mL Serum, SST/RTT, 1.0 mL GTT

Reported Time Same Day

Anion GapCalciumPhosphorusCalculated OsmolalityTotal ProteinAlbuminGlobulinAlbumin/Globulin ratio

Bilirubin TotalALP (Alkaline Phosphatase)AST (Aspartate Transaminase)GGT (Gamma-glutamyltransferase)CholesterolCK (Creatine Kinase)Sorbitol Dehydrogenase (SDH)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

8 Equine General Endocrine

Equine Chemistry Screen

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTTACTH Endo: 0.5 mL EDTA Plasma, Separated and Frozen, CTT.

Reported Time See Comments

ACTH Endogenous Total T4

Collection Notes For ACTH: Spin down LTT immediately and separate from cells. Freeze as soon as possible.Frozen plasma stable for 14 days. Avoid Grossly hemolyzed and/or lipemic samplesFor Chemistry: Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Dexamethasone suppression test is no longer recommended as the primary test for diagnosis of PPID/Cushing's Disease in horses.  Endogenous ACTH is the preferred test.  Chemistry Screen and Total T4 done same day.ACTH Endogenous run Mon/Wed/Fri with results out same day.Also see test code 6971 for information about TRH stimulation testing.

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12 Equine Immunodeficiency

CBC

Specimen Requirements IgG/IgM minimum: 0.2 mL Serum CBC: 1.0 mL LTT (0.5 mL minimum), Air dried peripheral blood smears

Reported Time See comment

Immunoglobulin M, Equine Immunoglobulin G, Equine

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Results for CBC will be out same day, IgG/IgM results out next day (24 hr final) A 4hr preliminary can be requested by calling laboratory.

1634 Equine Insulin/ACTH, Endogenous

Insulin

Specimen Requirements Insulin: 0.5 mL serum, Frozen, RTT/CTTEndogenous ACTH: 1.0 mL EDTA Plasma, separated and frozen, CTT.

Reported Time Mon/Wed/Fri, Same day

Glucose Endogenous ACTH

Collection Notes For ACTH, Endo: Spin down LTT immediately and separate from cells. Freeze as soon as possible. Frozen plasma stable for 14 days. Avoid Grossly hemolyzed and/or lipemic samples.For Insulin: Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection; this limits hemolysis which can affect results. Separate serum into transport tube and freeze.Avoid lipemia and hemolysis.

Additional Comments: Report includes reference ranges and interpretation. Equine insulin and glucose levels have been suggested for diagnosing insulin dysregulation, but can be abnormal in any disease causing insulin resistance. For more information, please visit our website to view our test service update on Equine insulin testing.Testing not recommended on samples >24 hours old due to rapid deterioration.

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750 Equine Pre-Op Panel

CBCFibrinogenGlucose

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT 1.0 mL GTT CBC: 1.5 mL LTT and air dried peripheral blood smears

Reported Time Same Day

BUN (Blood Urea Nitrogen)Total ProteinAlbumin

GlobulinGGT (Gamma-glutamyltransferase)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Fibrinogen performed by heat precipitation..

4 Equine Screen

CBCEquine Chemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.5 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Fibrinogen Plasma Protein

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: SDH is included in Equine Chemistry Screen.Fibrinogen performed by heat precipitation.

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89 Equine Screen, Follow-Up

CBCEquine Chemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.5 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Fibrinogen Plasma Protein

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: SDH is included in Equine Chemistry Screen. Fibrinogen performed by heat precipitation.

Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

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2404 Equine Senior Wellness I

CBCFibrinogenPlasma Protein

Specimen Requirements Chemistry/Insulin: 1.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.5 mL LTT and air dried peripheral blood smearsACTH Endo: 1.0 mL Frozen EDTA Plasma, CTT.Samples for Insulin testing CAN NOT be hemolyzed.

Reported Time See comment

InsulinGlucoseACTH Endogenous

Total T4Plasma Glucose

Collection Notes The LTT for ACTH Endo MUST be spun down within 30 minutes of collection and the plasma separated into a non-additive tube such as a CTT. Freeze until transported to lab. Insulin samples MUST be spun down within 30 minutes; if submission is to be delayed, draw a second tube or, volume allowing, pull some serum off the SST/RTT and Freeze for insulin testing.Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 30 minutes of collection; this then does not require collection of a GTT; this also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Equine insulin and glucose levels have been suggested for diagnosing insulin dysregulation, but can be abnormal in any disease causing insulin resistance. For more information, please visit our website to view our test service update on Equine insulin testing.Testing not recommended on samples >24 hours old due to rapid deterioration.

Chemistries and CBC reported out same day. ACTH Endo and Insulin run Monday/Wednesday/Friday, reported out same day.

Fibrinogen performed by heat precipitation.

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2405 Equine Senior Wellness II

CBCFibrinogen

Specimen Requirements Chemistry/Insulin: 2.0 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.5 mL LTT and air dried peripheral blood smearsACTH Endo: 1.0 mL Frozen EDTA Plasma, CTT. Samples for Insulin testing CAN NOT be hemolyzed.

Reported Time See comment

InsulinACTH Endogenous

Total T4Equine Chem Screen

Collection Notes The LTT for ACTH Endo MUST be spun down within 30 minutes of collection and the plasma separated into a non-additive tube such as a CTT. Freeze until transported to lab. Insulin samples MUST be spun down within 30 minutes; if submission is to be delayed, draw a second tube or, volume allowing, pull some serum off the SST/RTT and freeze for insulin testing. Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 30 minutes of collection; this then does not require collection of a GTT; this also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: SDH is included in Equine Chemistry Screen.Equine insulin and glucose levels have been suggested for diagnosing insulin dysregulation, but can be abnormal in any disease causing insulin resistance. For more information, please visit our website to view our test service update on Equine insulin testing.Testing not recommended on samples >24 hours old due to rapid deterioration.

Chemistries and CBC reported out day. ACTH, Endo and Insulin run Monday/Wednesday/Friday, reported out same day.

Fibrinogen performed by heat precipitation.

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2872 Equine Special w/ Insulin

CBCFibrinogen

Specimen Requirements Chemistry/Insulin: 1.0 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.5 mL LTT and air dried peripheral blood smears.

Reported Time See Comment

Total T4Equine Chem Screen

Insulin

Collection Notes Insulin samples MUST be spun down within 30 minutes; if submission is to be delayed, draw a second tube or, volume allowing, pull some serum off the SST/RTT and freeze for insulin testing. Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 30 minutes of collection; this then does not require collection of a GTT; this also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: SDH is included in Equine Chemistry Screen.Equine insulin and glucose levels have been suggested for diagnosing insulin dysregulation, but can be abnormal in any disease causing insulin resistance. For more information, please visit our website to view our test service update on Equine insulin testing.Testing not recommended on samples >24 hours old due to rapid deterioration.

Chemistry and CBC results out same day.Insulin run Monday/Wednesday/Friday with results out same day.

Fibrinogen performed by heat precipitation.

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40 Equine Total Body

CBCEquine Chemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.5 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urine

Reported Time Same Day

Fibrinogen Urinalysis

Collection Notes Please indicate method of urine collection. Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Submit sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: SDH is included in Equine Chemistry Screen.

Fibrinogen performed by heat precipitation.

123 Erythrocyte Sedimentation Rate (ESR)

Methodology: Wintrobe

Specimen Requirements 1.5 mL Whole Blood, LTT. Absolute minimum: 1.0 mL

Reported Time Same Day

Collection Notes None

Additional Comments: None

625 Ethylene Glycol

Methodology: Enzymatic

Specimen Requirements 0.5 mL Plasma, LTT/GrnTT

Reported Time Same Day

Collection Notes Separate from cells as soon as possible.

Additional Comments: Ethylene glycol levels peak 2 hours after ingestion in canines.

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236 Fecal Baermann

Methodology: Sedimentation

Specimen Requirements 3-5 grams of Fresh Feces, Fecal Tube or bag. For most accurate results, please submit fresh feces (less than 24 hours old).

Reported Time Next day

Collection Notes None

Additional Comments: Also known as Lungworm Fecal Baermann, this is a qualitative test, reporting presence or absence of lungworm larvae. This test does not rule out Filaroides sp. (Canine Lungworm).

To rule out Filaroides, submit bronchoalveolar lavage or transtracheal wash for cytology (318) or fecal sample for Ova and Parasite (213).

2241 Fecal Combo Screen w/ Giardia ELISA

Fecal Screen

Specimen Requirements 10 grams of Fresh Feces, Fecal Tube or bag.

Reported Time Next Day

Giardia ELISA

Collection Notes None

Additional Comments: Test includes sugar flotation with two centrifugation sequences; microscopic examination for ova and parasites, including Giardia cysts. Giardia ELISA reported out same day, fecal results out next day.

404 Fecal Fat

Methodology: Microscopic Examination

Specimen Requirements 3-5 grams of Fresh Feces, Fecal Tube or bag.

Reported Time Same Day

Collection Notes None

Additional Comments: Normal Test Value: Negative (normal stool contains little or no fat)

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37 Fecal Fat/Trypsin

Specimen Requirements 3-5 grams of Fresh Feces, Fecal Tube or bag.

Reported Time Same Day

Collection Notes None

Additional Comments: In dog and cats, serum TLI is more accurate in detecting pancreatic exocrine insuiciency.Refer to Canine TLI 68 or Feline TLI SO643.

Normal results are:Fecal Fat: Negative (normal stool contains little or no fat)Trypsin: Positive (normal stool should have some trypsin activity)

2409 Fecal Parasite Combo w/ Giardia/Crypto IFA

Fecal Screen

Specimen Requirements 10 grams of Fresh Feces, Fecal Tube or bag. Sample can not contain clay litter.

Reported Time See Comment

Giardia/Crypto IFA

Collection Notes Do not collect sample contaminated with clay kitty litter. It causes interference with IFA testing. Wheat/corn litter OK.

Additional Comments: Fecal float includes sugar flotation with two centrifugation sequences; microscopic examination for ova and parasites, including Giardia cysts. IFA testing will detect the antigen of Giardia species and most Cryptosporidia.It will not detect some species of Cryptosporidium that are associated with birds and reptiles.

For avian/non-mammalian testing, order Avian/Non-Mammalian Giardia/Cryptosporidium 1530.

409 Fecal Parasite Screen

Methodology: Centrifugation/Flotation

Specimen Requirements 5-10 grams of Fresh Feces, Fecal Tube or bag. For most accurate results, please submit fresh feces (less than 24 hours old).

Reported Time Next day

Collection Notes None

Additional Comments: Test includes sugar flotation with two centrifugation sequences; microscopic examination for ova and parasites, including Giardia cysts. Giardia/Crypto IFA (241) is recommended to confirm the presence of Giardia as noted.

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1409 Fecal Parasite, Quantitative

Methodology: Centrifugation/Microscopic

Specimen Requirements 1.0 gram Fresh Feces, Fecal tube or bag

Reported Time Next Day

Collection Notes None

Additional Comments: Results will be reported out as type of parasite and number of eggs per gram of feces.

405 Fecal Trypsin

Methodology: Gel Digestion

Specimen Requirements 3-5 grams of Fresh Feces, Fecal Tube or bag.

Reported Time Same Day

Collection Notes None

Additional Comments: Normal Test Value: Positive (normal stool should have some trypsin activity)

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1720 Feline ADR Panel

CBCChemistry ScreenUrinalysis

Specimen Requirements Chemistry: 1.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urineO&P/Gardia/Crypto: 5 grams fresh feces

Reported Time See Comment

FIPFeLVFIV

Ova and ParasiteGiardia/Crypto IFAFecal Culture

Collection Notes Please indicate method of urine collection. Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.For most accurate results, please submit fresh feces (less than 24 hours old). Do not collect sample contaminated with clay kitty litter. It causes interference with testing. Wheat/Corn litter OK.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: O&P: Test includes sugar flotation with two centrifugation sequences; microscopic examination for ova and parasites, including Giardia cysts. Giardia/Crypto IFA testing will detect the antigen of Giardia species and most Cryptosporidia. Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)Not recommended testing for juveniles. CBC, Chemistries, urinalysis, and FeLV run same day. FIP/FIV performed Mon - Sat AM, reported in afternoon. Fecal Culture reported in 36 - 72 hours; Fecal culture for the detection of Salmonella and Campylobacter. Shigella and Yersinia can be ruled out upon request. Additional charges may apply.

1612 Feline Calicivirus Titer

Methodology: IFA

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Monday, Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This test is for checking immunization status.

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209 Feline Coronavirus (FIP) Screen

Methodology: IFA

Specimen Requirements 0.5 mL Serum, SST/RTT. Test can also be run on abdominal fluid.

Reported Time Daily, AM

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This test is NOT specific for FIP. Testing performed at dilutions of 1:100 and 1:6400. For titered results see Coronavirus (FIP) titer 217.

Test can also be run on abdominal fluid, however, Body Cavity Fluid Cytology (#14) with Feline Coronavirus Antigen FA (SO529) recommended.

249 Feline Coronavirus (FIP) Screen, Add-On

Methodology: IFA

Specimen Requirements 0.5 mL Serum, SST/RTT. Minimum: 0.1 mL Serum.

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This test is NOT specific for FIP. Testing performed at dilutions of 1:100 and 1:6400.For titered results see Coronavirus (FIP) titer 217.

Test can also be run on abdominal fluid, however, Body Cavity Fluid Cytology (#14) with Feline Coronavirus Antigen FA (SO529) recommended.

Available as an add-on to an existing panel (within 7 days). Previous case ID is required to activate the discounted price.

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217 Feline Coronavirus (FIP), Titered

Methodology: IFA

Specimen Requirements 0.5 mL Serum, SST/RTT. Test can also be run on abdominal fluid.

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This is a quantitative titer testing method. It is NOT specific for FIP. Test involves eight serial dilutions ranging from 1:100 to 1:25,600.

Test can also be run on abdominal fluid, however, Body Cavity Fluid Cytology (#14) with Feline Coronavirus Antigen FA (SO529) recommended.

1629 Feline Heartworm Panel

Feline Heartworm Antibody

Specimen Requirements 0.5 mL Serum, SST/RTT and 1.0 mL Whole Blood LTT

Reported Time Same Day

Microfilaria (Knott's Test)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This test includes Feline Antibody test and microfilaria. Microfilaria are rarely detected in cats.For Feline heartworm antibody and antigen see Feline Heartworm Screen 2629

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2629 Feline Heartworm Screen

Methodology: RIM/ELISA

Feline Heartworm Antibody

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

Heartworm Antigen

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This test includes heartworm antibody and antigen and is recommended by the American Heartworm Society (www.heartwormsociety.com).

For Feline Antibody test and microfilaria order Feline Heartworm Panel 1629.

1611 Feline Herpesvirus Titer

Methodology: IFA

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Monday, Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This test is for checking immunization status.

161 Feline Immunization Screen I

Methodology: IFA

Feline Calicivirus

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Monday, Same Day

Feline Herpesvirus

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This screen is for checking immunization status. This screen is limited to feline calici and herpes virus. Also available are Immunization Screen II 1161, which screens for feline panleukopenia and herpes virus, and Immunization Screen III 2161, which screens for all three viruses.

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1161 Feline Immunization Screen II

Methodology: IFA

Feline Panleukopenia virus

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Monday, Same Day

Feline Herpesvirus

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This test is run every Monday with results out that same day. This screen is limited to feline panleukopenia and herpes virus.Also available are Immunization Screen I 161 which tests for feline calici and herpes virus, and Immunization Screen III 2161 which screens for all three viruses.Titer <1:50 recommend vaccination.

2161 Feline Immunization Screen III

Methodology: IFA

Feline Panleukopenia virus

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Monday, Same Day

Feline Herpesvirus Feline Calicivirus

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This screen is inclusive of Feline Immunization Screens I 161 and II 1161.Limited to feline panleukopenia, herpes, and calici viruses.

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859 Feline Panel

CBCChemistry Screen

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time See Comments

CK (Creatine Kinase)Total T4

FeLV (ELISA)FIV (IFA)FCoV (FIP) Screen (IFA)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistry, CBC and FeLV run same day. FIV/FIP run daily, AM. FIV Recommend repeat in 6-8 weeks, if result is weak positive. Vaccinated cats will have a positive antibody response indistinguishable from that of infected cats for at least one year post vaccination.

1613 Feline Panleukopenia Virus Titer

Methodology: IFA

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Monday, Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This test is for checking immunization status.

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4689 Feline PLI/Cobalamin/Folate

fPLI (Spec fPL™)

Specimen Requirements 1.5 mL Serum, SST/RTT. Sample MUST be non-hemolyzed for folate testing and avoid lipemic samples.

Reported Time Mon, Wed, Fri

Cobalamin Folate

Collection Notes Patient must be fasted 12-18 hours (diabetic animals 6-8 hours).Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Cover samples with foil, vet wrap, or a paper towel to protected from light (folate and cobalamin are light sensitive)

Additional Comments: Cobalamin and Folate are run in-house Monday, Wednesday, and Friday; reported out same day. fPLI (Spec fPL™) are shipped to Steiner GI Laboratory, Texas A&M University Monday, Wednesday, Thursday; turn around time is 4-7 days.

For canines order Canine PLI/Cobalamin/Folate 1013.

5 Feline Screen

Chemistry Screen

Specimen Requirements Chemistry: 0.75 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

CBC FeLV (ELISA)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: None

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1689 Feline TLI/PLI/Cobalamin/Folate

TLIfPLI (Spec fPL™)

Specimen Requirements 1.5 mL Serum, SST/RTT. Sample MUST be non-hemolyzed for folate testing and avoid lipemic samples.

Reported Time See comment

Cobalamin Folate

Collection Notes Patient must be fasted 12-18 hours (diabetic animals 6-8 hours).Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Cover samples with foil, vet wrap, or a paper towel to protected from light (folate and cobalamin can be light sensitive).

Additional Comments: Cobalamin and Folate are run in-house Monday, Wednesday, and Friday; reported out same day. TLI and fPLI (Spec fPL™) are shipped to Steiner GI Laboratory, Texas A&M University Monday, Wednesday, Thursday; turn around time is 4-7 days.For Canines see Canine TLI/PLI/Cobalamin/Folate 1012.

1696 Feline Toxoplasmosis IgG/IgM

Methodology: IFA

Specimen Requirements 0.2 mL Serum, SST/RTT

Reported Time Run M,W,F on days

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This test code is for felines only. Positive titers will be repeated andreported on the next run day

For other species see: Canine Toxoplasmosis IgG/IgM SO696 or Toxoplasmosis IgG/IgM, Other SO495

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865 Feline Viral I

FeLV (ELISA)

Specimen Requirements 0.3 mL Serum, SST/RTT.EDTA or Heparinized Plasma OK.

Reported Time See Comment

FIV (IFA)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: FeLV run same dayFIV run Daily, AM.Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

78 Feline Viral II

FeLV (ELISA)

Specimen Requirements 0.5 mL Serum, SST/RTT. EDTA or Heparinized Plasma OK.

Reported Time See Comments

FIV (IFA) FCoV (FIP) Screen (IFA)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: FeLV run same day. FIP/FIV performed Mon - Sat AM, reported in afternoon. This test is NOT specific for FIP. Testing performed at dilutions of 1:100 and 1:6400. For titered results see Coronavirus (FIP) titer 217Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

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860 Feline Viral III

FeLV (ELISA)FIV (IFA)

Specimen Requirements 1.0 mL Serum, SST/RTT and 0.5 mL LTT and/or air dried peripheral blood smears

Reported Time See Comments

FIP (FCoV) Screen (IFA) Mycoplasma hemofelis (Hemobartonella) (Smear Evaluation)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: FeLV and Mycoplasma hemofelis (Hemobartonella) run same day.FIV and FIP set up daily, AM. This test is NOT specific for FIP. Testing performed at dilutions of 1:100 and 1:6400. For titered results see Coronavirus (FIP) titer 217.Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)Mycoplasma hemofelis (Hemobartonella) test is a smear evaluation that will not diagnose occult infections. Consider Mycoplasma hemofelis (Hemobartonella felis) PCR testing SO1638 as clinically indicated.

321 FeLV (ELISA)

Methodology: ELISA

Specimen Requirements 0.5 mL Serum, SST/RTT. Can be performed on EDTA or heparinized Plasma.

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

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361 FeLV, Add-On

Methodology: ELISA

Specimen Requirements 0.5 mL Serum, SST/RTTCan be performed on EDTA or heparinized Plasma.

Reported Time Same Day

Collection Notes None

Additional Comments: Available as an add-on to an existing panel (within 7 days). Previous case ID is required to activate the discounted price.

325 Fibrinogen, Semi-quantitative

Methodology: Heat Precipitation

Specimen Requirements 0.5 mL Plasma (1.0 mL Whole Blood) LTT or BTT

Reported Time Same Day

Collection Notes None

Additional Comments: If a BTT is used, it will be corrected for the citrate dilution factor.

668 FIV

Methodology: IFA

Specimen Requirements 0.5 mL Serum, SST/RTT. Minimum: 0.1 mL serum.EDTA or heparinized plasma OK.

Reported Time Daily, Same Day

Collection Notes Please allow sample to clot (up to 20 minutes) and then spin down sample. If using a RTT, remove serum and put into a fresh RTT/CTT. Avoid lipemia and hemolysis.

Additional Comments: Positive results will be held for additional testing and will be resulted next day. Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

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768 FIV, Add-On

Methodology: IFA

Specimen Requirements 0.3 mL Serum, SST/RTT. EDTA or heparinized plasma OK.

Reported Time Same Day

Collection Notes None

Additional Comments: Positive results will be held for additional testing and will be resulted next day. Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

Available as add-on to existing panel (within 7 days). Previous case ID is required to activate the discounted price.

69 Folate

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum, SST/RTT. Sample MUST be non-hemolyzed for folate testing and avoid lipemic samples.

Reported Time Mon/Wed/Fri, Same day

Collection Notes Fast animals 12-18 hours (diabetic animals 6-8 hours) before draw.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Cover samples with foil, vet wrap, or a paper towel to protected from light (folate can be light sensitive).It is recommended to separate serum and freeze the sample if transport to lab is to be delayed.

Additional Comments: Folate is run in-house Monday, Wednesday, and Friday; reported out same day. Folates >24.0 ug/dL are re-run on dilution and reported.

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2247 Free T4

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

1247 Free T4,  Add-On

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Available as an add-on to an existing panel (within 7 days). Previous case ID is required to activate the discounted price.

195 Fructosamine

Methodology: Spectrophotometry

Specimen Requirements 0.5 mL Serum SST/RTT.

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Fructosamine reflects mean blood glucose concentrations of the proceeding two weeks.Along with other clinical information, fructosamine can aid in the differentiation between acute/transient hyperglycemia and persistent hyperglycemia associated with diabetes mellitus. Alterations of plasma protein turnover, hemolysis, hyperlipidemia, and thyroid status can affect fructosamine concentration.

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2 General Diagnostic

CBCGlucoseBUN (Blood Urea Nitrogen)CreatinineSodiumPotassiumSodium/Potassium RatioChloride

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Carbon Dioxide (CO2)Anion GapCalciumPhosphorusTotal ProteinAlbuminGlobulinA/G Ratio

Bilirubin, TotalALP (Alkaline Phosphatase)ALT (Alanine Transaminase)AST (Aspartate Transaminase)GGT (Gamma-glutamyltransferase)CholesterolCalculated Osmolality

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: None

857 General Diagnostic w/ Feline Viral I

CBCChemistry Screen

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time See Comment

FeLV (ELISA) FIV (IFA)

Test Alias: Feline Screen w/FIV

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistry, CBC and FeLV run same day. FIV run daily, AM. Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

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856 General Diagnostic W/ Feline Viral II

CBCChemistry Screen

Specimen Requirements Chemistry: 1.0 mL Serum,SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time See Comment

FeLV (ELISA)FIV (IFA)

FCoV (FIP) Screen (IFA)

Test Alias: Special Feline w/General Diag, (no Total T4)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistry, CBC and FeLV run same day. FIV/FIP run daily, AM.Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

77 General Diagnostic w/ T4

CBC

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Chemistry Screen Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

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8571 General Diagnostic w/ T4 and Feline Viral I

CBCChemistry Screen

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time See Comment

Total T4FeLV (ELISA)

FIV (IFA)

Test Alias: General Diag w/T4+FELV/FIV

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistry, CBC and FeLV run same day. FIV run daily, AM.Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

855 General Diagnostic w/ T4 and Feline Viral II

CBCChemistry Screen

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time See Comments

Total T4FeLV (ELISA)

FCoV (FIP) Screen (IFA)FIV (IFA)

Test Alias: Special Feline w/General Diagnostic, T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistry, CBC, and FeLV run same day, FIV/FIP run daily, AM.

Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted. Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

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2077 General Diagnostic w/ T4 and FeLV

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Total T4 FeLV

Test Alias: Spec Feline 77 w/FeLV

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

2072 General Diagnostic w/ Thyroid II

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Total T4 cTSH (Thyroid Stimulating Hormone)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: TSH only validated on canines, but has been used in felines.

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770 General Diagnostic w/ Triglycerides

CBC

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears.

Reported Time Same Day

Chemistry Screen Triglycerides

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: For triglycerides on body cavity fluid, see Body Cavity Fluid Triglycerides 2144

777 General Diagnostic w/T4 and Cobalamin/Folate

CBCChemistry Screen

Specimen Requirements Chemistry: 1.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears. Sample MUST be non-hemolyzed for folate testing and avoid lipemic samples.

Reported Time See additional comments Below

CobalaminFolate

Total T4

Collection Notes Patient must be fasted 12-18 hours (diabetic animals 6-8 hours). Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Cover samples with foil or a paper towel to protected from light (folate and cobalamin are light sensitive).Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistries and CBC run same day. Cobalamin and Folate are run in-house Monday, Wednesday, and Friday; reported out same day.Folates >24.0 ug/dL and cobalamin >1000 are re-run on dilution and reported.

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7711 General Diagnostic w/T4, Follow-Up

CBC

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Chemistry Screen Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

1283 General Diagnostic with Coagulation I

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsCoag: 1 BTT, See Collection Note for volume

Reported Time Same Day

PT (Prothrombin Time) APTT

Collection Notes Citrated tubes (BTT) provided by Phoenix Laboratory hold 2.7 mL of blood (to the top of the label). Tubes must be filled with 2.7 mL of blood. If collecting a smaller volume of blood (<2.7mL) the blood to anticoagulant ratio should be 9:1. Please call lab with any questions. Protocol: 1. Draw fresh venous blood into BTT until full (to top of label) but not beyond the top of the tube label. No clotting should be observed in the tube. 2. Cool to 4° C (refrigeration) until analysis. 3. If a delay greater than 24 hours is expected prior to analysis, then the BTT should be spun in a centrifuge, the plasma separated and placed in a CTT or other non-additive tube to be frozen until analysis.

For Chemistry: Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: The ratio of blood to anticoagulant is critical for valid coagulation results. Any clotting will results in the tube being rejected for analysis.

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72 General Diagnostic, Follow-Up

CBC

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Chemistry Screen

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

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61 General Endocrine, Canine

Chemistry Screen

Specimen Requirements 2 RTTs, Pre/Post 0.5 mL Serum Each, 1.0 mL GTTDO NOT USE SST TO SPIN DOWN SAMPLE

Reported Time Same Day

Total T4 ACTH Response (Pre and Post Cortisols)

Collection Notes A grey top tube is recommended but not necessary if the RTT is centrifuged and serum is transferred to a transport tube. PROTOCOL:1. Collect a resting sample in a RTT, 0.5 mL of serum is required. Separate serum if possible and refrigerate. 2. Inject 0.25 mg (250 µg) of Cortrosyn IM or IV (1 vial). 3. Collect a second sample EXACTLY 1 HOUR post injection in RTT, label clearly as to the collection status (post), and preserve in the same manner as the first sample. NOTE: The test has been validated using Cortrosyn. Although compounded gels are available, they vary greatly in peak times and are not recommended.

ALTERNATE CANINE PROTOCOL (Microdose): In order to keep Cortrosyn costs down, use the specific dose of 5 ug/kg IV - up to the maximum dose of 250 ug (one vial) per dog. Whatever is left over can then be split into aliquots of 25 ug or 50 ug using small plastic syringes. Normally 1 mL of sterile physiologic saline is used as diluent. If so, 25 ug would be 100 uL or 0.1 mL. ***When the patient is receiving Trilostane/Vetoryl, the timing of the test is important. The pre-cortisol sample should be drawn 3-5 hours after the morning capsule. The test is a one hour test, so in other words, the ACTH Response test should be completed 4-6 hours after the medication, which is usually given once a day in the morning.

Additional Comments: Pre and Post sample required. In addition to diagnosis of naturally occurring hyperadrenocorticism (Cushing's disease), this is the only diagnostic test for hypoadrenocorticism (Addison's disease), iatrogenic hyperadrenocorticism, and monitoring response to medical therapy of Cushing's disease. Prednisone, prednisolone, and other corticosteroids (except dexamethasone) will cross react. Recommend these drugs be discontinued at least 48 hrs before testing. If dog has been on prolonged steroids, patient must be tapered with the drug stopped for at least 3 weeks to let the adrenal gland recover. INTERPRETATION HYPERADRENOCORTICISM: An ACTH response test is done to CONFIRM clinical suspicion of hyperadrenocorticism. Stressful diseases (ill diabetics, renal failure, etc.) and current drug therapy may cause false positives. If the cortisol results are normal (and an adequate dose of Cortrosyn was administered) and there is high clinical suspicion of hyperadrenocorticism, sex hormone hyperadrenocorticism (atypical Cushing's) should be considered. Additional testing on the serum from this ACTH stimulation test can be performed. Please contact us within 7 days of this cortisol result for further testing options. A low-dose dexamethasone suppression test should also be considered, as some patients with Cushing's will be positive on one test but not on the other.INTERPRETATION HYPOADRENOCORTICISM: Pre and post cortisol <2 ug/dL are supportive of hypoadrenocorticism but need to be interpreted in light of other clinical findings and history. Failure to stimulate can occur with iatrogenic hyperadrenocorticism, dogs being treated for hyperadrenocorticism and Addison's disease.

For Feline Protocol See ACTH Response, Feline 1039

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119 GGT (Gamma-glutamyltransferase)

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

2401 Giardia ELISA

Methodology: ELISA

Specimen Requirements 2 grams of Fresh Feces, Fecal Tube or bag.

Reported Time Same Day

Collection Notes None

Additional Comments: None

240 Giardia/Cryptosporidium IFA

Methodology: IFA

Specimen Requirements 3-5 grams of Fresh Feces, Fecal Tube or bag. Sample can not contain clay litter.

Reported Time Daily, AM

Collection Notes Do not collect sample contaminated with clay kitty litter. It causes interference with testing. Wheat/corn litter OK.

Additional Comments: IFA testing will detect the antigen of Giardia species and most Cryptosporidia. It will not detect some species of Cryptosporidium that are associated with birds and reptiles.

For avian/non-mammalian testing, see Avian/Non-Mammalian Giardia/Cryptosporidium 1530.

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241 Giardia/Cryptosporidium IFA, add-on

Methodology: IFA

Specimen Requirements 3-5 grams of Fresh Feces, Fecal Tube or bag. Sample can not contain clay litter.

Reported Time Daily, AM

Collection Notes Do not collect sample contaminated with clay kitty litter. It causes interference with testing. Wheat/Corn litter OK.

Additional Comments: IFA testing will detect the antigen of Giardia species and most Cryptosporidia. It will not detect some species of Cryptosporidium that are associated with birds and reptiles. For avian/non-mammalian testing, see Avian/Non-Mammalian Giardia/Cryptosporidium 1530.

Available as an add-on to an existing panel (within 7 days). Previous case ID is required to activate the discounted price.

20 Glucose, Multiple Samples

Methodology: Spectrophotometry

Specimen Requirements 1.0 mL GTT for each sample, pre and post (up to 7 post samples)

Reported Time Same Day

Collection Notes Completely fill tube to allow for proper dilution. Or, collect samples in SSTs or RTTs. Centrifuge SST or RTT within 30 minutes of draw. Separate serum into transport tubes and refrigerate. The normal insulin and feeding regimen should be used during the glucose curve. Ideally, blood should be sampled every 2 hours between 2 insulin doses (for 12 hours if the animal is receiving insulin twice daily, for 24 hours if the animal is receiving insulin once daily).Ideally, blood samples should be collected every 2 hours for the glucose curve during as much of the day as possible (e.g. 8 am - 6 pm)

Additional Comments:

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2122 Glucose, Plasma

Methodology: Spectrophotometry

Specimen Requirements 0.25 Plasma, 1.0 mL GTT.Avoid hemolysis as it may cause falsely decreased values.

Reported Time Same Day

Collection Notes Be sure adequate volume is drawn, minimum, 1.0 mL. Plasma glucose may be falsely decreased due to insufficient volume.Centrifuge within 30 minutes of draw, can be poured off into fresh RTT/CTT following separation.

Additional Comments: None

121 Glucose, Serum

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

509 Gram Stain

Methodology: Microscopic Examination

Specimen Requirements Submit air-dried slides, swab, fluid or tissue.

Reported Time Next day

Collection Notes Collection site/source and species required for proper interpretation.

Additional Comments: Normal test values vary with culture site.

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629 Heartworm Antibody, Feline

Methodology: RIM

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Infections in felines are often single-sex worms in small numbers (1-3 worms). Antibodies are first detected in some cats at 50 -60 days. This test is the recommended Heartworm test for cats.To combine with Microfilaria, please order Feline Heartworm Panel #1629

238 Heartworm Antigen

Methodology: ELISA

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Testing recommended in dogs 7 months and older. Test sensitivity is limited to detection of adult female heartworm antigen. Immature heartworms, including microfilaria, are not detected, see Microfilaria 212.High worm burdens can have false negative results. If the result is unexpected, consider re-testing with heat treatment.Positive results verified by RIM test. This test is not recommended for use in cats as they usually have a low worm load (see Heartworm Antibody 629).To combine with microfilaria, order Heartworm Panel 58.

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2380 Heartworm Antigen, Add-On

Methodology: ELISA

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Testing recommended in dogs 7 months and older. Test sensitivity is limited to detection of adult female heartworm antigen. Immature heartworms, including microfilaria, are not detected, see Microfilaria 212.High worm burdens can have false negative results. If the result is unexpected, consider re-testing with heat treatment.Positive results verified by RIM test. This test is not recommended for use in cats as they usually have a low worm load (see Heartworm Antibody 629).To combine with microfilaria, order Heartworm Panel 58.Available as an add-on to an existing panel (within 7 days). Previous case ID is required to activate the discounted price.

419 Histopathology Additional Single Tissue - Full Report

Methodology: Histopathology

Specimen Requirements Additional 'Single Tissue'. Additional 1-4 tissue samples from one site (one surgical site or organ type), where length + width + height is less than 15cm in aggregate. Submitted in 10% neutral buffered formalin.

Reported Time 1-3 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Full report includes gross description, microscopic description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing.

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441 Histopathology Additional Single Tissue - Mini Report

Methodology: Histopathology

Specimen Requirements Additional 'Single Tissue'. Additional 1-4 tissue samples from one site (one surgical site or organ type), where length + width + height is less than 15cm in aggregate. Submitted in 10% neutral buffered formalin.

Reported Time 1-3 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Mini report includes gross description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing.

419x2 Histopathology Additional Single Tissue x2 - Full Report

Methodology: Histopathology

Specimen Requirements Two 'Additional Single Tissues' (2 x Additional 1-4 tissue samples from one surgical site or organ type, where length + width + height is less than 15cm in aggregate). Submitted in 10% neutral buffered formalin.

Reported Time 1-3 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Full report includes gross description, microscopic description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing.

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441X2 Histopathology Additional Single Tissue x2 - Mini Report

Methodology: Histopathology

Specimen Requirements Two 'Additional Single Tissues' (2 x Additional 1-4 tissue samples from one surgical site or organ type, where length + width + height is less than 15cm in aggregate). Submitted in 10% neutral buffered formalin.

Reported Time 1-3 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Mini report includes gross description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing.

419x3 Histopathology Additional Single Tissue x3 - Full Report

Methodology: Histopathology

Specimen Requirements Three 'Additional Single Tissues' (3 x Additional 1-4 tissue samples from one surgical site or organ type, where length + width + height is less than 15cm in aggregate). Submitted in 10% neutral buffered formalin.

Reported Time 1-3 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing.When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Full report includes gross description, microscopic description, diagnosis, and comments.Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing.

441X3 Histopathology Additional Single Tissue x3 - Mini Report

Methodology: Histopathology

Specimen Requirements Three 'Additional Single Tissues' (3 x Additional 1-4 tissue samples from one surgical site or organ type, where length + width + height is less than 15cm in aggregate). Submitted in 10% neutral buffered formalin.

Reported Time 1-3 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Mini report includes gross description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing.

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419x4 Histopathology Additional Single Tissue x4 - Full Report

Methodology: Histopathology

Specimen Requirements Four 'Additional Single Tissues' (4 x Additional 1-4 tissue samples from one surgical site or organ type, where length + width + height is less than 15cm in aggregate). Submitted in 10% neutral buffered formalin.

Reported Time 1-3 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Full report includes gross description, microscopic description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing.

441X4 Histopathology Additional Single Tissue x4 - Mini Report

Methodology: Histopathology

Specimen Requirements Four 'Additional Single Tissues' (4 x Additional 1-4 tissue samples from one surgical site or organ type, where length + width + height is less than 15cm in aggregate). Submitted in 10% neutral buffered formalin.

Reported Time 1-3 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Mini report includes gross description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing.

418 Histopathology Additional Skin Punch Biopsies - Full report

Methodology: Histopathology

Specimen Requirements Additional 1-4 skin punch biopsies. Submitted in 10% neutral buffered formalin.

Reported Time 1-3 Days, Reviewed M-F

Collection Notes Labelled mesh cassettes in formalin are preferred for small or friable tissues. Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Full report includes gross description, microscopic description, diagnosis, and comments.

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440 Histopathology Additional Skin Punch Biopsies - Mini report

Methodology: Histopathology

Specimen Requirements Additional 1-4 skin punch biopsies. Submitted in 10% neutral buffered formalin.

Reported Time 1-3 Days, Reviewed M-F

Collection Notes Labelled mesh cassettes in formalin are preferred for small or friable tissues.Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Mini report includes gross description, diagnosis, and comments.

1418 Histopathology Additional Skin Punches - Full, SLIDES ONLY

Methodology: Histopathology

Specimen Requirements H&E stained tissue slides - Additional 'Skin Punch Biopsies' (Additional 1-4 skin punch biopsies)

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Full report includes microscopic description, diagnosis, and comments.

This test is for received H&E slides ONLY; if submitting tissue sample in formalin, please see test code 418.

1440 Histopathology Additional Skin Punches - Mini, SLIDES ONLY

Methodology: Histopathology

Specimen Requirements H&E stained tissue slides - Additional 'Skin Punch Biopsies' (Additional 1-4 skin punch biopsies)

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Mini report includes diagnosis and comments.

This test is for received H&E slides ONLY; if submitting tissue samples in formalin, please see test code 440.

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1441 Histopathology Add'l Single Tissue - Mini, SLIDES ONLY

Methodology: Histopathology

Specimen Requirements H&E stained tissue slides - Additional 'Single Tissue' (1-4 samples from one surgical site or one organ type, where length + width + height is less than 15cm in aggregate)

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Mini report includes diagnosis and comments.

This test is for received H&E slides ONLY; if submitting tissue samples in formalin, please see test code 441.

1419X2 Histopathology Add'l Single Tissue x2 - Full, SLIDES ONLY

Methodology: Histopathology

Specimen Requirements H&E stained tissue slides - Two 'Additional Single Tissues' (2 x Additional 1-4 samples from one surgical site or organ type, where length + width + height is less than 15cm in aggregate)

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Full report includes microscopic description, diagnosis, and comments.

This test is for received H&E slides ONLY; if submitting tissue sample in formalin, please see test code 419x2.

1441X2 Histopathology Add'l Single Tissue x2 - Mini, SLIDES ONLY

Methodology: Histopathology

Specimen Requirements H&E stained tissue slides - Two 'Additional Single Tissues' (2 x Additional 1-4 samples from one surgical site or organ type, where length + width + height is less than 15cm in aggregate)

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Mini report includes diagnosis and comments.

This test is for received H&E slides ONLY; if submitting tissue samples in formalin, please see test code 441x2.

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1419X3 Histopathology Add'l Single Tissue x3 - Full, SLIDES ONLY

Methodology: Histopathology

Specimen Requirements H&E stained tissue slides - Three 'Additional Single Tissues' (3 x Additional 1-4 samples from one surgical site or organ type, where length + width + height is less than 15cm in aggregate)

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Full report includes microscopic description, diagnosis, and comments.

This test is for received H&E slides ONLY; if submitting tissue samples in formalin, please see test code 419x3.

1441X3 Histopathology Add'l Single Tissue x3 - Mini, SLIDES ONLY

Methodology: Histopathology

Specimen Requirements H&E stained tissue slides - Three 'Additional Single Tissues' (3 x Additional 1-4 samples from one surgical site or organ type, where length + width + height is less than 15cm in aggregate)

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Mini report includes diagnosis and comments.

This test is for received H&E slides ONLY; if submitting tissue samples in formalin, please see test code 441x3.

1419X4 Histopathology Add'l Single Tissue x4 - Full, SLIDES ONLY

Methodology: Histopathology

Specimen Requirements H&E stained tissue slides - Four 'Additional Single Tissues' (4 x Additional 1-4 samples from one surgical site or organ type, where length + width + height is less than 15cm in aggregate)

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Full report includes microscopic description, diagnosis, and comments.

This test is for received H&E slides ONLY; if submitting tissue samples in formalin, please see test code 419x4.

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1441X4 Histopathology Add'l Single Tissue x4 - Mini, SLIDES ONLY

Methodology: Histopathology

Specimen Requirements H&E stained tissue slides - Four 'Additional Single Tissues' (4 x Additional 1-4 samples from one surgical site or organ type, where length + width + height is less than 15cm in aggregate)

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Mini report includes diagnosis and comments.

This test is for received H&E slides ONLY; if submitting tissue samples in formalin, please see test code 441x4.

1419 Histopathology Add'l SingleTissue - Full, SLIDES ONLY

Methodology: Histopathology

Specimen Requirements H&E stained tissue slides - Additional 'Single Tissue' (Additional 1-4 samples from one surgical site or organ type, where length + width + height is less than 15cm in aggregate)

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Full report includes microscopic description, diagnosis, and comments.

This test is for received H&E slides ONLY; if submitting tissue sample in formalin, please see test code 419.

1403 Histopathology Complex Tissue - Full Path Review, SLIDES ONLY

Methodology: Histopathology

Specimen Requirements H&E stained tissue slide - Complex Tissue (Each 1-4 samples from one surgical site or one organ type, where length + width + height is greater than 15cm in aggregate. Each entire organ.)

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Full report includes microscopic description, diagnosis, and comments.

This test is for received H&E slides ONLY; if submitting tissue sample in formalin, please see test code 403.

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403 Histopathology Complex Tissue - Full Report

Methodology: Histopathology

Specimen Requirements Complex Tissue: 1-4 tissue samples from one site (one surgical site or organ type), where length + width + height is greater than 15cm in aggregate. Or each entire organ (examples: brain, heart, lung, spleen, liver, gallbladder, pancreas, 1 kidney, 1 eyeball, 1 ear canal, 1 mammary chain, 1-2 ovaries or testicles). Or each sample requiring decalcification (examples: 1 digit, tail, mandible).

Reported Time 2-5 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar, or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Full report includes gross description, microscopic description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing, while samples with bone or mineralization may require decalcification prior to processing.

1463 Histopathology Complex Tissue - Mini Path Review, SLIDES ONLY

Methodology: Histopathology

Specimen Requirements H&E stained tissue slide - Complex Tissue ( 1-4 samples from one surgical site or one organ type, where length + width + height is greater than 15cm in aggregate. Each entire organ.)

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Mini report includes diagnosis and comments.

This test is for received H&E slides ONLY; if submitting tissue samples in formalin, please see test code 463.

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463 Histopathology Complex Tissue - Mini Report

Methodology: Histopathology

Specimen Requirements Complex Tissue: 1-4 tissue samples from one site (one surgical site or organ type), where length + width + height is greater than 15cm in aggregate. Or each entire organ (examples: brain, heart, lung, spleen, liver, gallbladder, pancreas, 1 kidney, 1 eyeball, 1 ear canal, 1 mammary chain, 1-2 ovaries or testicles). Or each sample requiring decalcification (examples: 1 digit, tail, mandible).

Reported Time 2-5 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Mini report includes gross description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing, while samples with bone or mineralization may require decalcification prior to processing.

403x2 Histopathology Complex Tissue x2 - Full Report

Methodology: Histopathology

Specimen Requirements Two 'Complex Tissues'. Complex Tissue = 1-4 tissue samples from one site (one surgical site or organ type), where length + width + height is greater than 15cm in aggregate. Or each entire organ (examples: brain, heart, lung, spleen, liver, gallbladder, pancreas, 1 kidney, 1 eyeball, 1 ear canal, 1 mammary chain, 1-2 ovaries or testicles). Or each sample requiring decalcification (examples: 1 digit, tail, mandible).

Reported Time 2-5 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Full report includes gross description, microscopic description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing, while samples with bone or mineralization may require decalcification prior to processing.

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463x2 Histopathology Complex Tissue x2 - Mini Report

Methodology: Histopathology

Specimen Requirements Two 'Complex Tissues'. Complex Tissue = 1-4 tissue samples from one site (one surgical site or organ type), where length + width + height is greater than 15cm in aggregate. Or each entire organ (examples: brain, heart, lung, spleen, liver, gallbladder, pancreas, 1 kidney, 1 eyeball, 1 ear canal, 1 mammary chain, 1-2 ovaries or testicles). Or each sample requiring decalcification (examples: 1 digit, tail, mandible).

Reported Time 2-5 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Mini report includes gross description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing, while samples with bone or mineralization may require decalcification prior to processing.

403x3 Histopathology Complex Tissue x3 - Full Report

Methodology: Histopathology

Specimen Requirements Three 'Complex Tissues'. Complex Tissue = 1-4 tissue samples from one site (one surgical site or organ type), where length + width + height is greater than 15cm in aggregate. Or each entire organ (examples: brain, heart, lung, spleen, liver, gallbladder, pancreas, 1 kidney, 1 eyeball, 1 ear canal, 1 mammary chain, 1-2 ovaries or testicles). Or each sample requiring decalcification (examples: 1 digit, tail, mandible).

Reported Time 2-5 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Full report includes gross description, microscopic description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing, while samples with bone or mineralization may require decalcification prior to processing.

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463x3 Histopathology Complex Tissue x3 - Mini Report

Methodology: Histopathology

Specimen Requirements Three 'Complex Tissues'. Complex Tissue = 1-4 tissue samples from one site (one surgical site or organ type), where length + width + height is greater than 15cm in aggregate. Or each entire organ (examples: brain, heart, lung, spleen, liver, gallbladder, pancreas, 1 kidney, 1 eyeball, 1 ear canal, 1 mammary chain, 1-2 ovaries or testicles). Or each sample requiring decalcification (examples: 1 digit, tail, mandible).

Reported Time 2-5 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Mini report includes gross description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing, while samples with bone or mineralization may require decalcification prior to processing.

403x4 Histopathology Complex Tissue x4 - Full Report

Methodology: Histopathology

Specimen Requirements Four 'Complex Tissues'. Complex Tissue = 1-4 tissue samples from one site (one surgical site or organ type), where length + width + height is greater than 15cm in aggregate. Or each entire organ (examples: brain, heart, lung, spleen, liver, gallbladder, pancreas, 1 kidney, 1 eyeball, 1 ear canal, 1 mammary chain, 1-2 ovaries or testicles). Or each sample requiring decalcification (examples: 1 digit, tail, mandible).

Reported Time 2-5 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Full report includes gross description, microscopic description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing, while samples with bone or mineralization may require decalcification prior to processing.

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463x4 Histopathology Complex Tissue x4 - Mini Report

Methodology: Histopathology

Specimen Requirements Four 'Complex Tissues'. Complex Tissue = 1-4 tissue samples from one site (one surgical site or organ type), where length + width + height is greater than 15cm in aggregate. Or each entire organ (examples: brain, heart, lung, spleen, liver, gallbladder, pancreas, 1 kidney, 1 eyeball, 1 ear canal, 1 mammary chain, 1-2 ovaries or testicles). Or each sample requiring decalcification (examples: 1 digit, tail, mandible).

Reported Time 2-5 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Mini report includes gross description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing, while samples with bone or mineralization may require decalcification prior to processing.

407 Histopathology Limb Dissection

Methodology: Histopathology

Specimen Requirements Refrigerate limb immediately, or store on ice. Submit limb in plastic bags on ice. DO NOT freeze or fix.

Reported Time Preliminary Diagnosis: 3-5 days.Final Diagnosis: 7-14 days.

Collection Notes Please include history and radiographs. Securely seal and label plastic bags, with absorbent material in outermost bag.

Additional Comments: Full report includes gross description, microscopic description, diagnosis, and comments. Reporting time is dependent on soft tissue versus bone analysis.

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406 Histopathology Necropsy

Methodology: Histopathology

Specimen Requirements Refrigerate body, and submit for necropsy, as soon as possible. If refrigeration is not available, pack body with ice packs. Prevent freezing.

Reported Time Preliminary Diagnosis: 1-5 days.Final Diagnosis: 7-14 days, unless special studies are requested.

Collection Notes Please include as much history as possible.

Additional Comments: Please call the Lab for pricing and additional information. Full necropsy includes gross examination, microscopic description, diagnosis, ancillary inhouse lab testing as requested by pathologist, and comments. Gross necropsy is available at a reduced rate when the cause of death is obvious upon gross inspection. Spinal cord examination, toxicology, and cremation services are available at an additional cost.

1401 Histopathology Single Tissue - Full Path Review, SLIDES ONLY

Methodology: Histopathology

Specimen Requirements H&E stained tissue slides - Single Tissue (1-4 samples from one surgical site or one organ type, where length + width + height is less than 15cm in aggregate)

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Full report includes microscopic description, diagnosis, and comments.

This test is for received H&E slides ONLY; if submitting tissue sample in formalin, please see test code 401.

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401 Histopathology Single Tissue - Full Report

Methodology: Histopathology

Specimen Requirements Single Tissue: 1-4 tissue samples from one site (one surgical site or organ type), where length + width + height is less than 15cm in aggregate. Submitted in 10% neutral buffered formalin.

Reported Time 1-3 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline. Include absorbent material in the outermost bag.

Additional Comments: Full report includes gross description, microscopic description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing.

1400 Histopathology Single Tissue - Mini Path Review, SLIDES ONLY

Methodology: Histopathology

Specimen Requirements H&E stained tissue slide - Single Tissue (1-4 samples from one surgical site or one organ type, where length + width + height is less than 15cm in aggregate)

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Mini report includes diagnosis and comments.

This test is for received H&E slides ONLY; if submitting tissue sample in formalin, please see test code 400.

400 Histopathology Single Tissue - Mini Report

Methodology: Histopathology

Specimen Requirements Single Tissue: 1-4 tissue samples from one site (one surgical site or organ type), where length + width + height is less than 15cm in aggregate. Submitted in 10% neutral buffered formalin.

Reported Time 1-3 Days, Reviewed M-F

Collection Notes Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Mini report includes gross description, diagnosis, and comments. Reporting time is dependent on tissue type and size; larger samples require longer fixation and processing.

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425 Histopathology Skin Punch Biopsies - Full Report

Methodology: Histopathology

Specimen Requirements 1-4 Skin Punch Biopsies. Submitted in 10% neutral buffered formalin.

Reported Time 1-3 Days, Reviewed M-F

Collection Notes Labelled mesh cassettes in formalin are preferred for small or friable tissues.Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Full report includes gross description, microscopic description, diagnosis, and comments.

1425 Histopathology Skin Punch Biopsies - Full, SLIDES ONLY

Methodology: Histopathology

Specimen Requirements H&E stained tissue slides: 1-4 Skin punch biopsies

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Full report includes microscopic description, diagnosis, and comments.

This test is for received H&E slides ONLY; if submitting tissue samples in formalin, please see test code 425.

423 Histopathology Skin Punch Biopsies - Mini Report

Methodology: Histopathology

Specimen Requirements 1-4 Skin Punch Biopsies. Submitted in 10% neutral buffered formalin.

Reported Time 1-3 Days, Reviewed M-F

Collection Notes Labelled mesh cassettes in formalin are preferred for small or friable tissues.Securely seal and label formalin jar or plastic bags. If formalin fixation is not available, moisten tissue in saline and keep refrigerated prior to shipment. Prevent freezing. When shipping, please use minimal formalin/saline and include absorbent material in outermost bag.

Additional Comments: Mini report includes gross description, diagnosis, and comments.

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1423 Histopathology Skin Punch Biopsies - Mini, SLIDES ONLY

Methodology: Histopathology

Specimen Requirements H&E stained tissue slides: 1-4 Skin punch biopsies

Reported Time 1-3 Days, Reviewed M-F

Collection Notes None

Additional Comments: Pathologist review of previously H&E stained slides. Mini report includes diagnosis and comments.

This test is for received H&E slides ONLY; if submitting tissue samples in formalin, please see test code 423

813 Histopathology Special Stain

Methodology: Varies

Specimen Requirements Paraffin tissue block (preferred) or 2 unstained slides.

Reported Time 1-3 days, Performed M-F

Collection Notes None

Additional Comments: One special stain per one patient submission (up to 5 slides). Pricing based on histopathology previously completed at Phoenix Lab.

Please note: IHC stains are not included in this category. See test code 1667 Immunohistochemistry Stain.

813x2 Histopathology Special Stain x2

Methodology: Varies

Specimen Requirements Paraffin tissue block (preferred) or 4 unstained slides.

Reported Time 1-3 days, Performed M-F

Collection Notes None

Additional Comments: Two special stains per one patient submission (up to 5 slides per stain). Pricing based on histopathology previously completed at Phoenix Lab.

Please note: IHC stains are not included in this category. See test code 1667 Immunohistochemistry Stain.

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813x3 Histopathology Special Stain x3

Methodology: Varies

Specimen Requirements Paraffin tissue block (preferred) or 6 unstained slides.

Reported Time 1-3 days, Performed M-F

Collection Notes None

Additional Comments: Three special stains per one patient submission (up to 5 slides per stain). Pricing based on histopathology previously completed at Phoenix Lab.

Please note: IHC stains are not included in this category. Refer to test code 1667 for Immunohistochemistry Stain.

813x4 Histopathology Special Stain x4

Methodology: Varies

Specimen Requirements Paraffin tissue block (preferred) or 8 unstained slides.

Reported Time 1-3 days, Performed M-F

Collection Notes None

Additional Comments: Four special stains per one patient submission (up to 5 slides per stain). Pricing based on histopathology previously completed at Phoenix Lab.

Please note: IHC stains are not included in this category. Refer to test code 1667 for Immunohistochemistry Stain.

632 IgA Quantitative, Canine

Methodology: Radial Immunodiffusion (RID)

Specimen Requirements 0.3 mL Serum, SST/RTT

Reported Time 48 Hours

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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852 IgG, Camelid RID

Methodology: Radial Immunodiffusion (RID)

Specimen Requirements 0.3 mL Serum, SST/RTT

Reported Time 24 Hours

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Acceptable for Alpaca, Llama, and Camel. A 4-hr preliminary result is available upon request.

210 IgG, Equine RID

Methodology: Radial Immunodiffusion (RID)

Specimen Requirements 0.25 mL Serum, SST/RTT. Colostrum can also be used.

Reported Time 24 Hours

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: A 4-hr preliminary result is available upon request. Final result at 24 hours.

211 IgM, Equine RID

Methodology: Radial Immunodiffusion (RID)

Specimen Requirements 0.25 mL Serum, SST/RTT. Colostrum can also be used.

Reported Time 24 Hours

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Final result at 24 hours.

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13 Immune Mediated Hemolytic Anemia (IMHA) Panel

CBCCoombs

Specimen Requirements 1.0 mL Whole Blood LTT and air dried peripheral blood smears

Reported Time Same Day

Platelet Count Reticulocyte Count

Collection Notes Corticosteroids can cause false negative Coombs results if >7 days of therapy, therefore consider discontinuing corticosteroid use 2-3 weeks prior to testing.

Additional Comments: Coombs run on day shift only.Steroids will affect this test result, however, studies have shown that corticosteroid therapy of <7 days do not cause false negatives.

634 Insulin

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 1.0 mL Serum, Minimum: 0.5 mL, SST/RTT, Frozen**Serum can NOT be hemolyzed as hemolysis will interfere with testing.**

Reported Time Mon, Wed, Fri, Same day

Collection Notes Centrifuge tube within 20-30 minutes of collection. Separate serum into a transport tube. Sample should be tested or frozen within 24 hours. If unable to centrifuge, use GTT for collection of glucose samples. GTT tubes must have a minimum of 1 mL of blood for accurate results.

Additional Comments: In combination with clinical signs and concurrent blood glucose concentration, determination of serum insulin may be useful in diagnosing an insulinoma. In assessing whether the serum insulin concentration is appropriate, it must be compared to the serum glucose concentration (taken concurrently; included in the cost of insulin test). Measurement of serum insulin concentrations is most useful when the blood glucose concentration is 59 mg/dL or less.Equine insulin and glucose levels have been suggested for diagnosing pituitary adenoma/Cushing's disease, but can be abnormal in any disease causing insulin resistance. Testing not recommended on samples >24 hours old due to rapid deterioration.

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1718 Juvenile Routine Screen

CBCAlbuminALP (Alkaline Phosphatase)BUN (Blood Urea Nitrogen)Creatinine

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

BUN/Creat RatioGlobulinPotassiumALT (Alanine Transaminase)Total Protein

Albumin/Globulin ratioChlorideSodiumSodium/Potassium RatioGlucose

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Does not include a urinalysis.

12085 Large Animal Liver Chemistry

Methodology: Spectrophotometry

AlbuminALP (Alkaline Phosphatase)Total Bilirubin

Specimen Requirements 0.5 mL Serum SST/RTT

Reported Time Same Day

Sorbitol Dehydrogenase (SDH)Total ProteinGlobulin

Direct BilirubinGGT (Gamma-glutamyltransferase)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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385 Leptospirosis Rapid Screen

Methodology: RIM

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Leptospirosis is a zoonotic disease, transmitted by contact of the organism with mucous membranes. Take extra caution when handling animals/samples suspected of infection.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Test includes, but can not distinguish between, serovars Canicola, Grippotyphosa, Icterohaemorrhagiae, and Pomona. For additional Leptospirosis testing including MAT and PCR testing , see our Sendout Catalog.

124 Lipase

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

2217 Lipid Panel w/ T4

Methodology: Spectrophotometry

Cholesterol

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

Triglycerides Total T4

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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1084 Liver Chemistry Panel

Methodology: Spectrophotometry

AlbuminALP (Alkaline Phosphatase)ALT (Alanine Transaminase)AST (Aspartate Transaminase)

Specimen Requirements Chemistry: Two SST/RTT samples, Pre and Post, 0.5 mL each

Reported Time Same Day

Total ProteinGlobulinA/G RatioPre Bile AcidsPost Bile Acids

Bilirubin, DirectBilirubin, IndirectBilirubin, TotalGGT (Gamma-glutamyltransferase)

Collection Notes Pre Sample: 12 hr fastingPost: 2 hr post prandial.Use a standard meal such as maintenance or puppy formula food. Minimum recommendations = Feeding 2 tsp <10 lbs, 2 tbsp >10 lbs of canned, high quality food.PLEASE NOTE: The animal should be off Actigall (Ursodiol) 48 hours prior to running a bile acid study.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Post prandial values in normal dogs/cats do not usually exceed 20 umol/L. Bile acids testing is used to assess liver function.

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85 Liver Profile

CBCAlbuminALP (Alkaline Phosphatase)ALT (Alanine Transaminase)AST (Aspartate Transaminase)

Specimen Requirements Chemistry: Two SST/RTT samples, Pre and Post, 0.5 mL eachCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Total ProteinGlobulinA/G RatioPre Bile Acids SamplePost Bile Acids Sample

Bilirubin DirectBilirubin IndirectBilirubin TotalGGT (Gamma-glutamyltransferase)

Collection Notes Pre Sample: 12 hour fasting samplePost Sample: 2 hour post prandialUse a standard meal such as maintenance or puppy formula food. Minimum recommendations = Feeding 2 tsp <10 lbs, 2 tbsp >10 lbs of canned, high quality food.PLEASE NOTE: The animal should be off Actigall (Ursodiol) 48 hours prior to running a bile acid study.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Post prandial values in normal dogs/cats do not usually exceed 20 umol/L. Bile acids testing is used to assess liver function.

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620 Luteinizing Hormone

Methodology: RIM

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Please allow sample to clot (up to 20 minutes) and then spin down sample. If using a RTT, remove serum and put into a fresh RTT/CTT. Avoid lipemia and hemolysis.

Additional Comments: OVARIAN REMNANT DETERMINATION: Test when the dog is NOT showing signs of estrus: A positive result means high LH concentrations, indicating no ovarian tissue. (Occasionally false positives may occur with the 24 hr LH surge.) A negative result means low LH concentrations, indicating the presence of ovarian tissue. Exposure to estrogen creams will also cause a negative LH result.OVULATION TIMING IN THE BITCH: The duration of the LH peak varies from dog to dog and lasts only 24 hours in approximately 40% of the bitches. For this reason, serum should be tested for LH every day beginning on the 4th or 5th day of proestrus or when vaginal cytology approaches 50% cornification. When a positive result is obtained this is considered "Day 0". A progesterone test should be done on Day 0 and Day 3 to confirm a rise in progesterone. If the detected increase in LH was the actual preovulatory surge, progesterone concentrations should increase above 2 ng/mL within 3 days and stay elevated. If the progesterone remains low, less than 2 ng/mL, it indicates that a proestrus fluctuation in LH was identified. Continue daily testing until the true LH surge occurs. Days 4 through 7 post LH surge encompass the true fertile period with peak fertility on Days 5 and 6. Breeding on Days 2, 4, and 6 post LH surge will completely cover the fertile period and maximize viable sperm numbers on the bitch's most fertile days. Indeterminant results may indicate a negative result or that the LH surge is approaching or recently peaked. For animals with unknown ovarian status, this result may indicate that the animal has been ovariectomized. NOTE: This test has not been validated for, but has been used in, cats with similar interpretations as in dogs.

125 Magnesium

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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212 Microfilaria

Methodology: Modified Knotts

Specimen Requirements 1.0 mL Whole Blood, LTT

Reported Time Same Day

Collection Notes None

Additional Comments: Recommend testing only in dogs 7 months of age and older. Microfilaria are rarely detected in cats.Test included in Heartworm Panels, see Heartworm panel 58 or Feline Heartworm Panel 1629.

158 Milk Urea Nitrogen

Methodology: Spectrophotometry

Specimen Requirements 0.5 mL Milk, CTT

Reported Time Same Day

Collection Notes None

Additional Comments: None

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5318 Mini-Cytology

Methodology: Cytology

Specimen Requirements 3-5 air dried slides, unstained or tissue imprints

Reported Time Reviewed M-Sat on Days

Collection Notes FINE NEEDLE Aspirate PROTOCOL:1. If the cytology is a fine needle aspirate, use a 21g. -22 g. needle (smaller needles increase cell rupture) and 3-12 mL syringe depending on material/tissue aspirated. The softer the tissue, the smaller the syringe and needle necessary for aspiration. NOTE: Lymph nodes often do not require suction as this increases chance of rupture.2. Insert the needle into the mass (avoid the center of larger lesions as they are apt to be necrotic). In order to get the most representative sample the needle should be directed in at least 3 different directions with suction applied.3. Release the negative pressure slowly and withdraw the needle from the mass.4. Remove the needle,from syringe, fill the syringe with air, replace the needle and force the cellular material onto a slide or slides.5. Cover the material on the slide with another slide, allow the material to spread and then horizontally pull the slides apart without pressure. This must be done quickly to avoid clot formation.6. Quickly air dry the slides. DO NOT HEAT FIX. Try to make 3 - 5 slides from each lesion. Do not package the slides for transport until thoroughly dried. Do not refrigerate slides.

TISSUE IMPRESSION PROTOCOL:1. Impression smears can be made from an excised mass using the TOUCH IMPRINT TECHNIQUE. Blot tissue onto paper towel, then onto slides.2. Quickly air dry the slides. DO NOT HEAT FIX.3. Do not package the slides for transport until thoroughly dried. Do not refrigerate slides. Note that impression smears of ulcerated, dermal lesions are often of low diagnostic utility. VAGINAL CYTOLOGY FOR ESTRUS PROTOCOL:1. Insert the cotton tip swab against the caudodorsal surface of the vaginal vault (avoid the vulva and clitoral fossa) and advance the swab in a craniodorsal direction until it passes over the ischial arch. The swab should reach the pelvic canal.2. Rotate the swab 360 degrees in each direction and withdraw.3. For optimal exfoliation, gently roll the swab across the surface of slide, DO NOT SMEAR.4. Dry thoroughly before placing in slide container. Do not refrigerate slides.

Additional Comments: Complete history and location of lesion are essential for accurate results. Multiple lymph nodes are charged as one site. If the cytology is a paracentesis (abdominal, paracardial, or thoracic) or joint fluid with submission of fluid, refer to test Fluid Cytology (Body Cavity/ Joint Fluid) 14. Note that impression smears of ulcerated, dermal lesions are often of low diagnostic utility. EDTA (LTT) inhibits bacterial growth. If culture is required, use plain CTT or sterile transport tube.Reports will provide diagnosis and comments sections but will not contain a detailed microscopic description.

Price is for a single site. Every site after is an additional $21.00.

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530 Modified Acid Fast Stain

Methodology: Microscopic Examination

Specimen Requirements Bacterial culture swab with transport medium, tissue, feces, mucosal scraping, aspirate OR air-dried slides prepared with a thin film of one of the above.

Reported Time Next Day

Collection Notes None

Additional Comments: Also known as modified Kinyoun Acid Fast Stain. For the detection of Nocardia species.

328 Mycoplasma haemofelis (Hemobartonella)

Methodology: Microscopic Examination

Specimen Requirements 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Collection Notes None

Additional Comments: Exam of blood smear for Mycoplasma hemofelis (H. felis) is included in the CBC. Hemobartonella felis has been reclassified as Mycoplasma sp. Molecular testing (PCR) can differentiate Mycoplasma hemofelis (large form Hemobartonella) from Mycoplasma hemominutum (small form Hemobartonella). See test SO1638 Mycoplasma (Hemobartonella) hemofelis (PCR) for details.

259 Myoglobin, Urine

Methodology: Precipitation

Specimen Requirements 5 mL Urine, Urine Tube/CTT.Blood must be detected in urine to run test.

Reported Time Same Day

Collection Notes None

Additional Comments: Clinical signs, CBC, and serum biochemistry results can usually eliminate requirement for this test.

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341 Occult Blood

Methodology: Oxidation

Specimen Requirements 0.5 grams of Fresh Feces, Fecal Tube or bag.Fecal sample should be at least pea sized.See Collection Notes on dietary restrictions.

Reported Time Same Day

Collection Notes Patient should be on a high residue diet starting 48 hours prior to sample collection. (This excludes pet foods containing Red or rare meat or poultry products, raw fruits and vegetables containing high peroxidase activity. Please call our client service department with any questions.) High doses of vitamin C (>250 mg/day) can cause false negative results. Discontinue supplements, fruits and vegetables high in vitamin C for 48 hours prior to testing.

Additional Comments: Consuming a meat diet could result in false positives. See Collection Notes. Anti-inflammatory medicines and other diseases of the gastrointestinal tract may cause false positiveGastrointestinal lesions may bleed intermittently and blood in feces is not distributed uniformly, thus a negative results does not rule out the possability of gastrointestinal lesions.

8502 Oncology Profile

CBCGlucoseBUN (Blood Urea Nitrogen)

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same day

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)AlbuminCalcium

CreatinineTotal ProteinGlobulin

Collection Notes Please allow sample to clot (up to 20 minutes) and then spin down sample. If using a RTT, remove serum and put into a fresh RTT/CTT. Avoid lipemia and hemolysis.

A grey top tube is recommended but not necessary if the SST submitted has complete separation after centrifugation, or the RTT is centrifuged and serum is transferred to a transport tube.

Additional Comments: This test is for monitoring effects of chemotherapy.

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213 Ova & Parasite

Methodology: Centrifugation/Microscopic

Specimen Requirements 5 grams Fresh Feces, Fecal Tube or bag

Reported Time Next day

Collection Notes None

Additional Comments: Test includes a direct smear as well as a sugar flotation with two centrifugation sequences; microscopic examination for ova and parasites, including Giardia cysts. Giardia/Crypto IFA add-on #241 is recommended to confirm the presence of Giardia as noted.

2213 Ova & Parasite Combo w/IFA

Ova & Parasite

Specimen Requirements 10 grams of Fresh Feces, Fecal Tube or bag. Sample can not contain clay litter.

Reported Time Next Day

Giardia/Crypto IFA

Collection Notes Do not collect sample contaminated with clay kitty litter. It causes interference with IFA testing. Wheat/corn litter OK.

Additional Comments: Ova and parasite test includes a direct smear as well as a sugar flotation with two centrifugation sequences; microscopic examination for ova and parasites, including Giardia cysts. IFA testing will detect the antigen of Giardia species and most Cryptosporidia. It will not detect some species of Cryptosporidium that are associated with birds and reptiles.

For avian/non-mammalian testing, see Avian/Non-Mammalian Giardia/Cryptosporidium 1530.

239 Panleukopenia, Fecal

Methodology: RIM

Specimen Requirements 2 grams of Fresh Feces, Fecal Tube or bag.

Reported Time Same day

Collection Notes None

Additional Comments: FPV may only be detectable 24-48 hours post infection. False positives may occur up to two weeks post vaccination.

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215 Parvovirus Antibody, Serum

Methodology: IFA

Specimen Requirements 0.25 mL Serum, SST/RTT

Reported Time Daily, AM

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Used to detect parvovirus antibody, differentiating between IgG and IgM. Can also be used to evaluate for adequate titer post vaccine.

214 Parvovirus Antigen, Fecal

Methodology: RIM

Specimen Requirements 1 grams Fresh Feces, Fecal Tube or bag

Reported Time Same Day

Collection Notes None

Additional Comments: Used to evaluate for clinical infection in dogs only. Virus is shed for only a short-time.

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216 Phenobarbital

Methodology: Spectrophotometry

Specimen Requirements 0.5 mL Serum, RTT, peak OR trough sample

Reported Time Same Day

Collection Notes Drawn as single sample, trough or peak. Trough levels usually are most helpful. Trough:  Just prior to next dose   Peak: 4-6 hours post dose Protocol: 1. Select the desired dosing regimen and administer the drug until steady state is reached (2 weeks after change in dose). 2. Collect the sample as trough (preferably just prior to next dose) or peak (preferably 4-6 hours post dose)Please allow all serum samples (RTT) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.Remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: In dogs with well-controlled seizures, after steady state is reached (2 weeks after a change in dose), trough levels of phenobarbital should be monitored periodically. Ideally, these samples should be collected as a fasting sample prior to the next dose (i.e. if the dog receives phenobarbital at 8am and 8pm, the blood should be collected at 8am or 8pm, prior to phenobarbital administration). When toxicity is suspected, (or sometimes in animals with poorly controlled seizures), trough and peak serum phenobarbital concentration determination may be helpful, see Pheonbarbital, Peak and Trough 79.The time of peak blood phenobarbital concentrations is variable; usually a sample collected 6 hours after phenobarbital administration may be considered adequate for peak serum phenobarbital concentration.The half-life of phenobarbital varies widely among individual animals, therefore monitoring of serum levels aids in establishing an appropriate dose. Phenobarbital concentrations are also useful for evaluating owner compliance. Phenobarbital is the active component of primidone.

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79 Phenobarbital, Peak & Trough

Methodology: Spectrophotometry

Phenobarbital, Peak

Specimen Requirements 0.5 mL Serum, 2 RTT, sample Label accordingly (Peak/Trough)

Reported Time Same Day

Phenobarbital, Trough

Collection Notes Protocol:1. Select the desired dosing regimen and administer the drug until steady state is reached (2 weeks after change in dose).2. Collect the trough sample (preferably just prior to next dose) and collect the peak sample (preferably 6 hours post dose).

Please allow all serum samples (RTT) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.Remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: In dogs with well-controlled seizures, after steady state is reached (2 weeks after a change in dose), trough levels of phenobarbital should be monitored periodically. Ideally, these samples should be collected as a fasting sample prior to the next dose (i.e. if the dog receives phenobarbital at 8am and 8pm, the blood should be collected at 8am or 8pm, prior to phenobarbital administration). When toxicity is suspected, (or sometimes in animals with poorly controlled seizures), trough and peak serum phenobarbital concentration determination may be helpful. The time of peak blood phenobarbital concentrations is variable; usually a sample collected 6 hours after phenobarbital administration may be considered adequate for peak serum phenobarbital concentration.The half-life of phenobarbital varies widely among individual animals, therefore monitoring of serum levels aids in establishing an appropriate dose. Phenobarbital concentrations are also useful for evaluating owner compliance. Phenobarbital is the active component of primidone.

126 Phosphorus

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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344 Plasma Protein

Methodology: Refractometry

Specimen Requirements 0.25 mL EDTA Plasma (0.75 mL Whole Blood)

Reported Time Same Day

Collection Notes None

Additional Comments: This is actually a measurement of the total solids in serum and is only an estimate of the total plasma protein. High concentrations of glucose, urea, Na+, Cl+, and lipid may falsely increase results

345 Platelet Count, Automated

Methodology: Flow Cytometry

Specimen Requirements 0.5 mL Whole Blood, LTT.

Reported Time Same Day

Collection Notes For the most accurate results, recommend drawing from the largest vein possible (unless contraindicated), and mixing well by gently inverting tube x10.

Additional Comments: Sample can not be run if platelet clumping or clots are present.

Included in CBC.

376 Platelet Count, Manual

Hemocytometer

Specimen Requirements 0.5 mL Whole Blood, LTT

Reported Time Same Day

Collection Notes For the most accurate results, recommend drawing from the largest vein possible (unless contraindicated), and mixing well by gently inverting tube x10.

Additional Comments: Sample can not be run if platelet clumping or clots are present.

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127 Potassium

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

10850 Pre-Op Chemistry Screen

Methodology: Spectrophotometry

BUN (Blood Urea Nitrogen)CreatinineAlbumin

Specimen Requirements 0.5 mL Serum SST/RTT, 1.0 mL GTT

Reported Time Same Day

GlucoseTotal ProteinGlobulin

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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850 Pre-Op Panel

CBCGlucoseBUN (Blood Urea Nitrogen)

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

CreatinineTotal ProteinGlobulin

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)Albumin

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: None

1851 Pre-Op Panel, Follow-up

CBCGlucoseBUN (Blood Urea Nitrogen)

Specimen Requirements Chemistry: 0.5 mL Serum,SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

CreatinineTotal ProteinAlbumin

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)Globulin

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

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851 Pre-Op Panel, Large Animal

CBCGlucoseBUN (Blood Urea Nitrogen)

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

CreatinineTotal ProteinAlbumin

ALP (Alkaline Phosphatase)AST (Aspartate Transaminase)Globulin

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: None

547 Progesterone, Canine

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum, RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.Remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Canine: To best predict optimal breeding dates, serial progesterone results should be interpreted in conjunction with Vaginal Cytology (Canine Breeding Panel, Test 1647). At ovulation, serum progesterone concentration ranges from 3.8-10 ng/mL. Optimal breeding (fresh semen) is 2 days after ovulation (if frozen semen: 4 days after ovulation). For timing of whelping, progesterone decreases to <2 ng/mL 36-48 hours prior to whelping. After the initial test, follow-up testing is offered; order Progesterone Follow-up, Canine 747.

This test code is for canine use only. All other species use Progesterone test code #647

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747 Progesterone, Follow-Up

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum, RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.Remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This is to be used for canine prebreeding or prewhelping if multiple testing is needed.

Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

647 Progesterone, Non-Canine

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum, RTTMinimum: 0.3 mL Serum

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.Remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Mare: Corpus luteal values are 7-8 ng/mL by 6 days after ovulation. There is a transient decrease at 28 days of pregnancy to around 5 ng/mL and then concentration increases to day 50 and plateaus at day 140 (15-20 ng/mL). Values then decline so that at 180-200 days levels are below 1 ng/mL. Cow: Levels of 6 ng/mL are required to maintain pregnancy. Camelids: Values of about 2.0 ng/mL indicate luteal activity.

For canines, please order Canine Progesterone 547

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31 Protein Electrophoresis

Agarose Gel Electrophoresis

Specimen Requirements 0.25 mL Serum, SST/RTT. Can be run on heparinized plasma for avians/nonmammalians.

Reported Time Monday and Thursday, Same Day

Collection Notes None

Additional Comments: Test can also be performed on body cavity effusions to assist in diagnosis of FIP.

346 Prothrombin Time (PT)

Methodology: Endpoint Clot Detection

Specimen Requirements 0.5 mL Citrated PlasmaSee Collection notes for details about proper BTT Volume.

Reported Time Same Day

Collection Notes Citrated tubes (BTT) provided by Phoenix Laboratory hold 2.7 mL of blood (to the top of the label). Tubes must be filled with 2.7 mL of blood. If collecting a smaller volume of blood (<2.7mL) the blood to anticoagulant ratio should be 9:1.Protocol: 1. Draw fresh venous blood into BTT until full (to top of label) but not beyond the top of the tube label. No clotting should be observed in the tube. 2. Cool to 4° C (refrigeration) until analysis. 3. If a delay greater than 24 hours is expected prior to analysis, then the BTT should be spun in a centrifuge, the plasma separated and placed in a CTT or other non-additive tube to be frozen until analysis.

Additional Comments: The ratio of blood to anticoagulant is critical for valid coagulation results. Any clotting will results in the tube being rejected for analysis.

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300 Relaxin, Canine or Feline

Methodology: RIM

Specimen Requirements 0.5 mL Serum, SST/RTT.Test can also be run on heparinized or EDTA Plasma (GrnTT/LTT).Markedly hemolyzed or lipemic samples should not be used.

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Day 20 of gestation is found to be the earliest day of pregnancy detection. Relaxin rises after implantation of a fertilized egg. Implantation occurs 26-31 days after the LH surge. Any negative result is considered indicative of non-pregnancy; however, two negative results one week apart may be required for confirmation of this status, especially when date of mating is unknown. A positive result is considered indicative of pregnancy. A weak positive result may be observed at the beginning of the pregnancy. It is recommended to repeat the test on a fresh sample one week later to confirm pregnancy.

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1724 Relocated Canine Routine Screen

CBCUrinalysisVector/Tick Borne Disease ScreenAlbuminGlobulinAlb/Glob Ratio

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urine

Reported Time Same day

GlucoseBUN ( Blood Urea Nitrogen)CreatinineBUN/Crea RatioTotal proteinHeartworm Panel (in 6 months)

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)SodiumPotassiumChloride

Collection Notes Please indicate method of urine collection.Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Use code 17246 and include original accession number when submitting the 6 month follow up Canine Heartworm Panel. Follow Up testing must be submitted within 12 months.

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1723 Relocated Feline Routine Screen

CBCUrinalysisHeartworm AntibodyAlbuminGlobulinAlb/Glob Ratio

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urine

Reported Time Same Day

GlucoseBUN ( Blood Urea Nitrogen)CreatinineBUN/Crea RatioTotal proteinFeline Heartworm Screen (in 6 months)

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)SodiumPotassiumChloride

Collection Notes Please indicate method of urine collection.Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Use code 17236 and include original accession number when submitting the 6 month follow up Feline Heartworm Panel. Follow Up testing must be submitted within 12 months.

5023 Renal Panel, Chronic

Methodology: Spectrophotometry

BUN (Blood Urea Nitrogen)Creatinine

Specimen Requirements 0.3 mL Serum, SST/RTT. Please call our Client Services for information about minimums on short samples.

Reported Time Same Day

Phosphorus Potassium

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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5025 Renal Profile

HCT (Hematocrit)Creatinine

Specimen Requirements 0.5 mL of each, Serum, SST/RTT0.1 mL Whole Blood, LTT.A sealed crit w/ blood also accepted in lieu of LTT.

Reported Time Same Day

PotassiumCalcium

PhosphorusBUN (Blood Urea Nitrogen)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Run every 3 to 6 months depending on clinical signs.

5027 Renal Profile w/ Urinalysis

UrinalysisHCT (Hematocrit)BUN (Blood Urea Nitrogen)

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTTUrinalysis: 5 mL fresh urine, Urine Tube/CTT

Reported Time Same Day

CreatininePhosphorus

PotassiumCalcium

Collection Notes Please indicate method of urine collection.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Run every 3 to 6 months depending on clinic signs.

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1188 Reptile Mini Liver Panel

Methodology: Spectrophotometry

Total ProteinAlbumin

Specimen Requirements 0.1 mL Serum, m-SST/m-RTT Heparinized plasma or 0.25 mL whole blood from m-GrnTT is also accepted.Please call our Client Services for information about minimums on short samples.

Reported Time Same Day

CK (Creatine Kinase)Bile Acids

AST (Aspartate Transaminase)GGT (Gamma-glutamyltransferase)

Collection Notes None

Additional Comments: None

5026 Reptile Mini Renal Panel

Methodology: Spectrophotometry

Uric AcidCa/Phos Ratio

Specimen Requirements 0.1 mL Serum, m-SST/m-RTT Heparinized plasma or 0.25 mL whole blood from m-GrnTT is also accepted.Please call our Client Services for information about minimums on short samples.

Reported Time Same Day

Phosphorous Calcium

Collection Notes None

Additional Comments: None

87 Reptile Profile

WBC Est/DiffHematocrit (HCT)GlucoseCalcium

Specimen Requirements Chemistry: 0.3 mL (minimum: 100 µL), heparinized plasma or 250 µL Whole BloodCBC: 2 air dried slides and 0.1 mL (Absolute minimum: 30 µL) of either heparinized or EDTA whole blood. Heparin is preferred for most species.Please call our Client Services for information about minimums on short samples.

Reported Time Same Day

PhosphorusBile AcidTotal ProteinCholesterol

Uric AcidCa/Phos RatioAST (Aspartate Transaminase)

Collection Notes One m-GrnTT can be used, volume allowing. Submit m-GrnTT without centrifugation in order for HCT and chemistries to be done an same tube. Prepare slides at clinic.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: If a microtainer (M-GrnTT or M-LTT) is not submitted, WBC is estimated from the blood smear.

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88 Reptile Profile, Follow-up

WBC Est/DiffHematocrit (HCT)GlucoseCalcium

Specimen Requirements Chemistry: 0.3 mL (minimum: 100 µL), heparinized plasma or 250 µL Whole BloodCBC: 2 air dried slides and 0.1 mL (Absolute minimum: 30 µL) of either heparinized or EDTA whole blood. Heparin is preferred for most species.Please call our Client Services for information about minimums on short samples.

Reported Time Same Day

PhosphorusBile AcidTotal ProteinCholesterol

Uric AcidCa/Phos RatioAST (Aspartate Transaminase)

Collection Notes One m-GrnTT can be used, volume allowing. Submit m-GrnTT without centrifugation in order for HCT and chemistries to be done an same tube. Prepare slides at clinic.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: If a microtainer (M-GrnTT or M-LTT) is not submitted, WBC is estimated from the blood smear.

Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

2231 Resting Cortisol

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum, RTT

Reported Time Same Day

Collection Notes If the patient has been on steroids for >14 days, patient should be taperdown and discontinue for at least 2 weeks prior to testing. If <14 days, discontinue for 38 hours prior to testing.DO NOT USE SST TO COLLECT OR SPIN DOWN SAMPLE

Additional Comments: A patient with a resting cortisol >2.0 ug/dL has a 99% chance of NOT having Addison's disease.With a resting cortisol of <2.0 ug/dL, an ACTH Response 39 is needed to rule-in Addison's disease. This interpretation is valid if no oral or topical steroids have been given in the last 6 weeks. A resting cortisol cannot be used to rule- in or out hyperadrenocorticism. A LDDS 38 and/or ACTH Response 39 needs to be performed.

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6 Ruminant Screen

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.5 mL LTT and air dried peripheral blood smears

Reported Time Same Day

FibrinogenMagnesium

CK (Creatine Kinase)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Fibrinogen performed by heat precipitation

1106 Ruminant Screen, Follow-Up

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL serum; CBC: 1.0 mL LTT, Air dried peripheral blood smears

Reported Time Same Day

MagnesiumFibrinogen

CK (Creatine Kinase)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Fibrinogen performed by heat precipitation

Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

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5010 RX - ACE Inhibitor

UrinalysisPotassium

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT Urinalysis: 5 mL fresh urine

Reported Time Same Day

BUN (Blood Urea Nitrogen) Creatinine

Collection Notes Please indicate method of urine collection.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Run after 1 week, 1 month, then every 3 to 6 months.

4006 RX - Addison I (DOCP/Florinef)

Methodology: Spectrophotometry

BUN (Blood Urea Nitrogen)Creatinine

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

SodiumPotassium

Sodium/Potassium Ratio

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Once therapy has begun, run every 1 to 2 weeks, then every 3 to 4 months after stabilized.

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4008 RX - Addisons II (DOCP/Florinef and Pred)

BUN (Blood Urea Nitrogen)CreatinineALP (Alkaline Phosphatase)

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

PotassiumSodium

ALT (Alanine Transaminase)GGT (Gamma-glutamyltransferase)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Once therapy has begun, run every 1 to 2 weeks, then every 3 to 4 months after stabilized.

4005 RX - Amitriptyline

CBC

Specimen Requirements Chemistry: 0.25 mL Serum, SST/RTTCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

ALP (Alkaline Phosphatase) ALT (Alanine Transaminase)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Run 1 month after starting drug, then yearly.

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5018 RX - Amlodipine

UrinalysisBUN (Blood Urea Nitrogen)

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTTUrinalysis: 5 mL fresh urine

Reported Time Same Day

CreatinineTotal Bilirubin

PotassiumALT (Alanine Transaminase)

Collection Notes Please indicate method of urine collection.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Run 1 week after starting drug or adjusting dose, then 1 month later. Thereafter, run every 3 to 6 months.

5020 RX - Calcitriol I

Methodology: Spectrophotometry

UrinalysisBUN (Blood Urea Nitrogen)

Specimen Requirements 0.3 mL Serum, SST/RTT

Reported Time Same Day

CalciumAlbumin

PhosphorusCreatinine

Collection Notes Please indicate method of urine collection.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Run at 1 week,after starting, 1 month, then every 2 to 4 months

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5029 RX - Calcitrol II

Methodology: Spectrophotometry

BUN (Blood Urea Nitrogen)Creatinine

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

CalciumAlbumin

Phosphorus

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Run at 1 week,after starting, 1 month, then every 2 to 4 months

4002 RX - Cyclosporine

CBCCyclosporine A levelGlucose

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsCyclosporine: 1 mL LTT

Reported Time See Comment

BUN (Blood Urea Nitrogen)Creatinine

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: CBC and Chemistries done same day. Cyclosporine turn around time is 3-5 days. For more information about cyclosporine testing, see Cyclosporine A Level SO1640 in our sendout catalogue.

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5012 RX - Diabetes Mellitus I

Urinalysis

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTTUrinalysis: 5 mL fresh urine

Reported Time Same Day

Glucose Fructosamine

Collection Notes Please indicate method of urine collection.Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Run one month after starting treatment,then every 3 to 4 months, more often ifpatient's condition dictates.

5030 RX - Diabetes Mellitus II

Methodology: Spectrophotometry

Glucose

Specimen Requirements 0.5 mL Serum, STT/RTT, 1.0 mL GTT

Reported Time Same Day

Fructosamine

Collection Notes Prolonged contact with cells can dramatically affect glucose values.We strongly encourage that all serum samples be allowed to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.

Additional Comments: Run one month after starting treatment,then every 3 to 4 months, more often ifpatient's condition dictates.

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4004 RX - Diazepam

Methodology: Spectrophotometry

ALP (Alkaline Phosphatase)Bilirubin, Total

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

AST (Aspartate Transaminase) ALT (Alanine Transaminase)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Once therapy has begun, run after 5 days, 15 days, then every 3 to 6 months.

5031 RX - Digoxin I

DigoxinUrinalysisTotal T4Calcium

Specimen Requirements Chemistry and Digoxin -1.5 mL Serum, RTT onlyUrinalysis: 5 mL fresh urine

Reported Time See Comments

BUN (Blood Urea Nitrogen)CreatinineAlbumin

SodiumPotassiumChloride

Collection Notes Please indicate method of urine collection.DO NOT USE SST TO COLLECT OR SPIN DOWN SAMPLEPlease allow all serum samples (RTT) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.Remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Run 3-5 days after starting or changing dose, then every 4 to 6 months. Chemistries and urinalysis run same day. Digoxin turn around time is 3-5 days.

Please See Digoxin SO525 for additional information.

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5007 RX - Digoxin II

DigoxinUrinalysisChloride

Specimen Requirements Chemistry: 0.5 mL Serum, RTTUrinalysis: 5.0 mL urineDigoxin: 1.0 mL Serum, RTT ONLY

Reported Time See Comment

BUN (Blood Urea Nitrogen)CreatinineNa/K Ratio

PotassiumSodium

Test Alias: RX - Digoxin

Collection Notes Please indicate method of urine collection. DO NOT USE SST TO COLLECT OR SPIN DOWN SAMPLEPlease allow all serum samples (RTT) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.Remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Run 3-5 days after starting or changing dose, then every 4 to 6 months. Chemistries and urinalysis run same day. Digoxin turn around time is 3-5 days.

Please See Digoxin SO525 for additional information.

5017 RX - Diuretics

CBCBUN (Blood Urea Nitrogen)Creatinine

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTTCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

SodiumPotassiumChloride

CalciumAlbumin

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Run 1 week after starting drug or adjusting dose, then 1 month later.Thereafter, every 3 to 6 months.

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5022 RX - Fluoxetine/Prozac

Methodology: Spectrophotometry

Bilirubin, Total

Specimen Requirements 0.3 mL Serum, SST/RTT

Reported Time Same Day

ALP (Alkaline Phosphatase) ALT (Alanine Transaminase)

Test Alias: RX - Prozac

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Run 1 month after starting drug, then every 3 to 6 months

4003 RX - Gentamicin

UrinalysisGentamicin

Specimen Requirements Chemistry: 0.5 mL serum, RTTUrinalysis: 5 mL urineGentamicin: 1.0 mL serum, RTT ONLY

Reported Time See Comments

BUN (Blood Urea Nitrogen) Creatinine

Test Alias: Aminoglycosides

Collection Notes Please indicate method of urine collection. DO NOT USE SST TO COLLECT OR SPIN DOWN SAMPLEPlease allow all serum samples (RTT) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.Remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Urinalysis and Chemistry done same day. Gentamicin turn around time 3-5 Days. For more information about gentamicin testing see Gentamicin SO707 in our sendout catalogue.

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4001 RX - Methimazole I

CBCUrine Specific GravityTotal T4

Specimen Requirements Chemistry: 0.5 mL serum, SST/RTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrine SG 1.0 mL urine

Reported Time Same Day

BUN (Blood Urea Nitrogen)Creatinine

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)

Test Alias: RX - Tapazole I

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Run at 1 month, then every 4 to 6 months. Generic: Methimazole, Brand: Tapazole, Felimazole

4007 RX - Methimazole II

CBCTotal T4

Specimen Requirements Chemistry: 0.5 mL serum, SST/RTTCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

ALP (Alkaline Phosphatase) ALT (Alanine Transaminase)

Test Alias: TDM Tapazole II

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Run at 1 month, then every 4 to 6months.Generic: Methimazole, Brand: Tapazole, Felimazole

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5005 RX - NSAIDS II

UrinalysisTotal ProteinAlbumin

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTTUrinalysis: 5 mL fresh urine

Reported Time Same day

BUN (Blood Urea Nitrogen)CreatinineBilirubin, Total

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)

Collection Notes Please indicate method of urine collection.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Run baseline prior to starting drug, then run after 2 weeks, 1 month and thenevery 3 to 6 monthsNSAIDS include: Rimadyl, Ectogesic, Deramaxx, Metacam, Xubrin, Previcox

5006 RX - Phenobarb II

Methodology: Spectrophotometry

PhenobarbitalAlbuminBilirubin, Total

Specimen Requirements 1.0 mL Serum, RTT Only

Reported Time Same Day

ALT (Alanine Transaminase)AST (Aspartate Transaminase)Total Protein

GGT (Gamma-glutamyltransferase)ALP (Alkaline Phosphatase)

Collection Notes DO NOT USE SST TO COLLECT OR SPIN DOWN SAMPLEPlease allow all serum samples (RTT) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.Remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Recommended to run every 6 months

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2216 RX - Phenobarbital and KBR

Bilirubin, TotalPhenobarbital

Specimen Requirements 1.5 mL Serum, RTT. DO NOT USE SST TO COLLECT OR SPIN DOWN SAMPLE.

Reported Time See Comment

AST (Aspartate Transaminase)ALT (Alanine Transaminase)

Potassium Bromide

Test Alias: Phenobarb TDM2

Collection Notes See Test Components for collection protocol.DO NOT USE SST TO COLLECT OR SPIN DOWN SAMPLEPlease allow all serum samples (RTT) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.Remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: In dogs with well-controlled seizures, after steady state is reached (2 weeks after a change in dose), trough levels of phenobarbital should be monitored periodically. Ideally, these samples should be collected as a fasting sample prior to the next dose (i.e. if the dog receives phenobarbital at 8am and 8pm, the blood should be collected at 8am or 8pm, prior to phenobarbital administration). When toxicity is suspected, (or sometimes in animals with poorly controlled seizures), trough and peak serum phenobarbital concentration determination may be helpful, see Pheonbarbital, Peak and Trough 79.The time of peak blood phenobarbital concentrations is variable; usually a sample collected 6 hours after phenobarbital administration may be considered adequate for peak serum phenobarbital concentration.The half-life of phenobarbital varies widely among individual animals, therefore monitoring of serum levels aids in establishing an appropriate dose. Phenobarbital concentrations are also useful for evaluating owner compliance. Phenobarbital is the active component of primidone.Potassium Bromide test performed by Clinical Pharmacology Laboratory, Auburn University. For more information, see Potassium bromide SO531.

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5008 RX - Phenobarbital I

CBCAlbuminPhenobarbitalTotal Protein

Specimen Requirements Chemistry: 1.0 mL Serum, RTTCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)AST (Aspartate Transaminase)

GGT (Gamma-glutamyltransferase)Total Bilirubin

Test Alias: RX - Pheno

Collection Notes DO NOT USE SST TO COLLECT OR SPIN DOWN SAMPLEPlease allow all serum samples (RTT) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.Remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Recommended to run every 6 months

5013 RX - Theophylline

TheophyllineBUN (Blood Urea Nitrogen)Creatinine

Specimen Requirements 1.5 mL Serum, RTT OnlySerum must be collected using RTT.

Reported Time See Comment

ChlorideALT (Alanine Transaminase)Potassium

GGT (Gamma-glutamyltransferase)Bilirubin, TotalSodium

Test Alias: RX - Methyl

Collection Notes DO NOT USE SST TO COLLECT OR SPIN DOWN SAMPLEPlease allow all serum samples (RTT) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.Remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Please See Theophylline SO93 for additional information.

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5021 RX - Thyroxine/Soloxine

Methodology: Spectrophotometry

CBC

Specimen Requirements 0.3 mL Serum, SST/RTT

Reported Time Same Day

Total T4 Cholesterol

Test Alias: RX - Soloxine

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Run after 1 month, then every 3 to 6 months.(Levothyroxine, Soloxine)Same generic brand as methimazole

5011 RX- Cushing's (Trilostane, Mitotane)

CBCUrinalysisACTH Response

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTTCBC: 1.0 mL LTTACTH Response: 0.5 mL Serum each, Pre and Post, from RTT ONLY

Reported Time Same Day

CreatinineSodiumPotassium

BUN (Blood Urea Nitrogen)ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)

Test Alias: RX - Cushings

Collection Notes DO NOT USE SST TO COLLECT OR SPIN DOWN SAMPLE for ACTH Response Test.

Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.Remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Please see ACTH Response 39 for collection protocol.

Additional Comments: Run prior to and immediately after induction, then every 3 to 4 months, more if patient's condition dictates.

Please see ACTH Response 39 for additional information.

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3001 RX-Heparin

CBCAPTTAlbuminALP (Alkaline Phosphatase)Bilirubin, Total

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTTCBC: 1.0 mL LTT and air dried peripheral blood smearsCoag: 1 BTT Citrated Whole BloodTHE RATIO OF BLOOD TO ANTICOAGULANT IS CRITICAL FOR VALID COAGULATION RESULTS. See Collection Notes.

Reported Time Same Day

BUN (Blood Urea Nitrogen)CalciumCholesterolCreatinineTotal Protein

GGT (Gamma-glutamyltransferase)GlobulinPhosphorusPotassium

Collection Notes Citrated tubes (BTT) provided by Phoenix Laboratory hold 2.7 mL of blood (to the top of the label). Tubes must be filled with 2.7 mL of blood. If collecting a smaller volume of blood (<2.7mL) the blood to anticoagulant ratio should be 9:1.Protocol: 1. Draw fresh venous blood into BTT until full (to top of label) but not beyond the top of the tube label. No clotting should be observed in the tube. 2. Cool to 4° C (refrigeration) until analysis. 3. If a delay greater than 24 hours is expected prior to analysis, then the BTT should be spun in a centrifuge, the plasma separated and placed in a CTT or other non-additive tube to be frozen until analysis.For Chemistry: Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: The ratio of blood to anticoagulant is critical for valid coagulation results. Any clotting will results in the tube being rejected for analysis.Fibrinogen performed by heat precipitation.

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5009 RX-NSAIDS I

CBCUrinalysisAlbumin

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urine

Reported Time Same Day

CreatinineTotal BilirubinTotal Protein

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)BUN (Blood Urea Nitrogen)

Test Alias: RX - NSAIDS

Collection Notes Please indicate method of urine collection.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Run baseline prior to starting drug, then run after 2 weeks, 1 month and thenevery 3 to 6 monthsNSAIDS include: Rimadyl, Ectogesic, Deramaxx, Metacam, Xubrin, Previcox

32 Semen Evaluation

Cytology

Specimen Requirements 1.0 mL Semen, LTT/CTT

Reported Time Reviewed M-Sat on Days

Collection Notes For canines only: Collect entire ejaculate for accurate reporting of sperm total per ejaculate.

Additional Comments: Includes sperm count, pH and cytology.Please note that sperm motility should be assessed immediately after sample collection and will not be assessed with this cytology.

***For Canines, Equines, and Bovines only.***

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1716 Senior Routine Screen

CBCGlucoseBUN (Blood Urea Nitrogen)CreatinineBUN/Creat RatioSodiumPotassium

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urine

Reported Time Same Day

Sodium/Potassium RatioChlorideCalciumPhosphorusTotal ProteinAlbuminGlobulin

Albumin/Globulin ratioBilirubin TotalALP (Alkaline Phosphatase)ALT (Alanine Transaminase)Total T4Urinalysis

Collection Notes Please indicate method of urine collection. Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: None

11 Senior Screen

Methodology: Spectrophotometry

GlucosePotassiumPhosphorusChloride

Specimen Requirements 0.5 mL Serum SST/RTT

Reported Time Same Day

BUN (Blood Urea Nitrogen)CreatinineNa/K RatioTotal T4

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)Sodium

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

For a Senior Screen w/ CBC, order 899

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4705 Senior Screen w/ Calcium and HCT

GlucosePotassiumSodiumPhosphorusHCT

Specimen Requirements 0.5 mL of each, Serum, SST/RTT, 1.0 mL GTT, and 0.1 mL Whole Blood, LTT. A sealed crit w/ blood also accepted in lieu of LTT.

Reported Time Same Day

CreatinineChlorideTotal T4Calcium

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)BUN (Blood Urea Nitrogen)Na/K Ratio

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

899 Senior Screen w/ CBC

CBCGlucosePotassiumSodium

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

PhosphorusBUN (Blood Urea Nitrogen)CreatinineChloride

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)Na/K RatioTotal T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

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3047 Senior Screen W/ CBC and Free T4

CBCGlucosePotassiumSodiumPhosphorus

Specimen Requirements Chemistry: 0.75 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

CreatinineChlorideTotal T4Free T4

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)BUN (Blood Urea Nitrogen)Na/K Ratio

Sr Feline w/CBC, FreeT4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

1866 Senior Screen w/ Free T4

GlucosePotassiumSodiumNa/K RatioPhosphorus

Specimen Requirements 0.5 mL Serum SST/RTT

Reported Time Same Day

BUN (Blood Urea Nitrogen)CreatinineTotal T4Chloride

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)Free T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

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2071 Senior Screen W/ TSH

GlucosePotassiumSodiumPhosphorus

Specimen Requirements 0.5 mL Serum, SST/RTT, 1 mL GTT

Reported Time Same Day

BUN (Blood Urea Nitrogen)CreatinineChlorideTotal T4

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)Na/K RatioTSH (Thyroid Stimulating Hormone)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: TSH only validated on canines, but has been used in felines. Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

4701 Senior Screen w/Calcium

Methodology: Spectrophotometry

GlucosePotassiumSodiumPhosphorus

Specimen Requirements 0.5 mL Serum, SST/RTT, 1.0 mL GTT

Reported Time Same Day

CreatinineChlorideTotal T4Calcium

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)BUN (Blood Urea Nitrogen)Na/K Ratio

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

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1101 Senior Screen w/Cholesterol

Methodology: Spectrophotometry

GlucosePotassiumSodiumNa/K Ratio

Specimen Requirements 0.5 mL Serum SST/RTT, 1.0 mL GTT

Reported Time Same Day

BUN (Blood Urea Nitrogen)CreatinineTotal T4Chloride

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)CholesterolPhosphorus

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

2120 Serum Proteins

Methodology: Spectrophotometry

Albumin

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

Globulin Total Protein

Test Alias: Globulin Screen

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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101004 Small Mammal Chem Screen II

Methodology: Spectrophotometry

GlucoseBUN (Blood Urea Nitrogen)CreatinineCalciumPhosphorusTotal ProteinBilirubin, Total

Specimen Requirements Chemistry: 1  m-SST or m-GrnTT Minimum: 250 uL serum/plasma OR 550 µL whole blood for non-ferrets (1 mL whole blood for ferrets).

Reported Time Same Day

AST (Aspartate Transaminase)CK (Creatine Kinase)CholesterolAmylaseLipaseBile Acids

AlbuminGlobulinA/G RatioALP (Alkaline Phosphatase)GGT (Gamma-glutamyltransferase)ALT (Alanine Transaminase)

Test Alias: Ferret/Small Mammal 20-Chem

Collection Notes Please call our Client Services for information about minimums on short samples.

Additional Comments: Chemistry screen does NOT includes: electrolytes, carbon dioxide, osmolality, or anion gap.

956 Small Mammal Chemistry Screen I

Methodology: Spectrophotometry

GlucoseBUN (Blood Urea Nitrogen)CreatinineCalcium

Specimen Requirements 0.3 mL, m-SST/m-RTT, (Minimum: 150 µL serum) Heparinized plasma or 350 µL whole blood for non-ferrets(0.6 mL whole blood for ferrets) m-GrnTT OKPlease call our Client Services for information about minimums on short samples.

Reported Time Same Day

PhosphorusTotal ProteinAlbuminGlobulin

A/G RatioALT (Alanine Transaminase)AST (Aspartate Transaminase)

Test Alias: Ferret/Small Mammal 12-Chem Panel

Collection Notes None

Additional Comments: None

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101002 Small Mammal Comprehensive Chemistry Screen

Methodology: Spectrophotometry

GlucoseBUN (Blood Urea Nitrogen)CreatinineSodiumPotassiumSodium/Potassium RatioChlorideCarbon Dioxide (CO2)Anion Gap

Specimen Requirements 2  m-SST or m-GrnTT, Minimum: 300 uL serum/plasma OR 650 µL whole blood for non-ferrets (1 mL whole blood for ferrets).

Reported Time Same Day

CalciumPhosphorusCalculated OsmolalityTotal ProteinAlbuminGlobulinAlbumin/GlobulinBilirubin, Total

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)AST (Aspartate Transaminase)GGT (Gamma-glutamyltransferase)CholesterolCK (Creatine Kinase)AmylaseLipaseBile Acids

Test Alias: Ferret/Small Mammal 27-Chem

Collection Notes Please call our Client Services for information about minimums on short samples.

Additional Comments: None

1002 Small Mammal Comprehensive Panel

CBCGlucoseBUN (Blood Urea Nitrogen)CreatinineSodiumPotassiumSodium/Potassium RatioChlorideCarbon Dioxide (CO2)Anion Gap

Specimen Requirements Chemistry: 2  m-SST or m-GrnTT, Minimum: 300 uL serum/plasma OR 650 µL whole blood for non-ferrets (1 mL whole blood for ferrets).CBC: 2 air dried slides, and 1 m-LTT or m-GrnTT Minimum: 250 uL  whole blood

Reported Time Same Day

CalciumPhosphorusCalculated OsmolalityTotal ProteinAlbuminGlobulinAlbumin/GlobulinBilirubin, Total

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)AST (Aspartate Transaminase)GGT (Gamma-glutamyltransferase)CholesterolCK (Creatine Kinase)AmylaseLipaseBile Acids

Test Alias: Ferret/Small Mammal 27-Chem w/CBC

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Please call our Client Services for information about minimums on short samples.

Additional Comments: If a microtainer (m-GrnTT or m-LTT) is not submitted, WBC is estimated from the blood smear.

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10022 Small Mammal Comprehensive Panel w/ Protein Electrophoresis

CBCPEP (Protein Electrophoresis)GlucoseBUN (Blood Urea Nitrogen)CreatinineSodiumPotassiumSodium/Potassium RatioChlorideCarbon Dioxide (CO2)Anion Gap

Specimen Requirements Chemistry: 2  m-SST or m-GrnTT Minimum: 300 uL serum or plasma OR 650 µL whole blood for non-ferrets (1 mL whole blood for ferrets).CBC: 2 air dried slides, and 1 m-LTT or m-GrnTT Minimum: 250 uL  whole blood for CBC

Reported Time Same Day, PEP Mon/Thur Same Day

CalciumPhosphorusCalculated OsmolalityTotal ProteinAlbuminGlobulinAlbumin/GlobulinBilirubin, Total

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)AST (Aspartate Transaminase)GGT (Gamma-glutamyltransferase)CholesterolCK (Creatine Kinase)AmylaseLipaseBile Acids

Test Alias: Ferret/Small Mammal 27-Chem w/CBC and PEP

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Please call our Client Services for information about minimums on short samples.

Additional Comments: If a microtainer (m-GrnTT or m-LTT) is not submitted, WBC is estimated from the blood smear. Protein Electrophoresis is run Monday and Thursdays, with results out same day

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11002 Small Mammal Comprehensive Panel, Follow-Up

CBCGlucoseBUN (Blood Urea Nitrogen)CreatinineSodiumPotassiumSodium/Potassium RatioChlorideCarbon Dioxide (CO2)Anion Gap

Specimen Requirements Chemistry: 2  m-SST or m-GrnTT, Minimum: 300 uL serum/plasma OR 650 µL whole blood for non-ferrets (1 mL whole blood for ferrets).CBC: 2 air dried slides, and 1 m-LTT or m-GrnTT Minimum: 250 uL  whole blood

Reported Time Same Day

CalciumPhosphorusCalculated OsmolalityTotal ProteinAlbuminGlobulinAlbumin/GlobulinBilirubin, Total

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)AST (Aspartate Transaminase)GGT (Gamma-glutamyltransferase)CholesterolCK (Creatine Kinase)AmylaseLipaseBile Acids

Test Alias: Ferret/Small Mammal 27-Chem/CBC, Follow-up

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Please call our Client Services for information about minimums on short samples.

Additional Comments: If a microtainer (m-GrnTT or m-LTT) is not submitted, WBC is estimated from the blood smear.

Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

1186 Small Mammal Mini Liver Panel

Methodology: Spectrophotometry

CK (Creatine Kinase)Bilirubin Total

Specimen Requirements 0.15 mL Serum m-SST/m-RTT. Heparinized plasma or 0.35 mL whole blood (0.6 mL for ferrets) from m-GrnTT is also accepted.

Reported Time Same Day

ALP (Alkaline Phosphatase)ALT (Alanine Transaminase)

AST (Aspartate Transaminase)GGT (Gamma-glutamyltransferase)

Collection Notes Please call our Client Services for information about minimums on short samples.

Additional Comments: None

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5028 Small Mammal Mini Renal Panel

Methodology: Spectrophotometry

CreatinineBUN (Blood Urea Nitrogen)Sodium

Specimen Requirements 0.15 mL Serum, m-SST/m-RTT. Heparinized plasma or 0.35 mL whole blood (0.6 mL for ferrets) from m-GrnTT is also accepted.

Reported Time Same Day

ChloridePotassiumCalcium

PhosphorousTotal ProteinAlbumin

Collection Notes Please call our Client Services for information about minimums on short samples.

Additional Comments: None

56 Small Mammal Panel I

CBCGlucoseBUN (Blood Urea Nitrogen)CreatinineCalcium

Specimen Requirements 0.3 mL, m-SST/m-RTT, (Minimum: 150 µL serum), Heparinized plasma or 350 µL whole blood for non-ferrets(0.6 mL whole blood for ferrets) m-GrnTT OKCBC: Minimum: 250 uL EDTA whole blood (heparinized whole blood OK), 2 air dried slides.Please call our Client Services for information about minimums on short samples.

Reported Time Same Day

PhosphorusTotal ProteinAlbuminGlobulin

A/G RatioALT (Alanine Transaminase)AST (Aspartate Transaminase)

Test Alias: Ferret/Small Mammal 12-Chem w/CBC

Collection Notes Please call our Client Services for information about minimums on short samples.

Additional Comments: If a microtainer (m-GrnTT or m-LTT) is not submitted, WBC is estimated from the blood smear.

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84 Small Mammal Panel I, Follow-Up

CBCGlucoseBUN (Blood Urea Nitrogen)Creatinine

Specimen Requirements Chemistry: 0.3 mL, m-SST/m-RTT, Minimum: 150 uL serum (heparinized plasma OK) or 350 µL whole blood for non-ferrets, 0.6 mL whole blood for ferrets.CBC: Minimum: 250 uL EDTA whole blood (heparinized whole blood OK), 2 air dried slides.Please call our Client Services for information about minimums on short samples.

Reported Time Same Day

CalciumPhosphorusTotal ProteinAlbumin

GlobulinA/G RatioALT (Alanine Transaminase)AST (Aspartate Transaminase)

Ferret/Small Mammal 12-Chem/CBC, Follow-up

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Please call our Client Services for information about minimums on short samples.

Additional Comments: If a microtainer (M-GrnTT or M-LTT) is not submitted, WBC is estimated from the blood smear. Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

1004 Small Mammal Panel II

CBCGlucoseBUN (Blood Urea Nitrogen)CreatinineCalciumPhosphorusBilirubin, Total

Specimen Requirements Chemistry: 1  m-SST or m-GrnTT Minimum: 250 uL serum/plasma OR 550 µL whole blood for non-ferrets (1 mL whole blood for ferrets).CBC: 2 air dried slides, and 1 m-LTT or m-GrnTT Minimum: 250 uL  whole blood

Reported Time Same Day

AST (Aspartate Transaminase)CK (Creatine Kinase)CholesterolAmylaseLipaseBile Acids

Total ProteinAlbuminGlobulinA/G RatioALP (Alkaline Phosphatase)GGT (Gamma-glutamyltransferase)ALT (Alanine Transaminase)

Test Alias: Ferret/Small Mammal 20-Chem w/CBC

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Please call our Client Services for information about minimums on short samples.

Additional Comments: Panel does NOT includes: electrolytes, carbon dioxide, osmolality, or anion gap. If a microtainer (m-GrnTT or m-LTT) is not submitted, WBC is estimated from the blood smear.

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10044 Small Mammal Panel II w/ Protein Electrophoresis

CBCPEP (Protein Electrophoresis)GlucoseBUN (Blood Urea Nitrogen)CreatinineCalciumPhosphorus

Specimen Requirements Chemistry: 1  m-SST or m-GrnTT Minimum: 250 uL serum/plasma OR 550 µL whole blood for non-ferrets (1 mL whole blood for ferrets).CBC: 2 air dried slides, and 1 m-LTT or m-GrnTT Minimum: 250 uL  whole blood

Reported Time Same Day

AST (Aspartate Transaminase)CK (Creatine Kinase)CholesterolAmylaseLipaseBile AcidsBilirubin, Total

Total ProteinAlbuminGlobulinA/G RatioALP (Alkaline Phosphatase)GGT (Gamma-glutamyltransferase)ALT (Alanine Transaminase)

Test Alias: Ferret/Small Mammal 20-Chem w/CBC & PEP

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Please call our Client Services for information about minimums on short samples.

Additional Comments: Panel does NOT includes: electrolytes, carbon dioxide, osmolality, or anion gap. If a microtainer (m-GrnTT or m-LTT) is not submitted, WBC is estimated from the blood smear. Protein electrophoresis is run Monday and Thursday, with results out same day.

11004 Small Mammal Panel II, Follow-Up

CBCGlucoseBUN (Blood Urea Nitrogen)CreatinineCalciumPhosphorusBilirubin, Total

Specimen Requirements Chemistry: 1  m-SST or m-GrnTT Minimum: 250 uL serum/plasma OR 550 µL whole blood for non-ferrets (1 mL whole blood for ferrets).CBC: 2 air dried slides, and 1 m-LTT or m-GrnTT Minimum: 250 uL  whole blood

Reported Time Same Day

AST (Aspartate Transaminase)CK (Creatine Kinase)CholesterolAmylaseLipaseBile Acids

Total ProteinAlbuminGlobulinA/G RatioALP (Alkaline Phosphatase)GGT (Gamma-glutamyltransferase)ALT (Alanine Transaminase

Test Alias: Ferret/Small Mammal 20-Chem/CBC, Follow-up

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Please call our Client Services for information about minimums on short samples.

Additional Comments: Panel does NOT includes: electrolytes, carbon dioxide, osmolality, or anion gap. If a microtainer (m-GrnTT or m-LTT) is not submitted, WBC is estimated from the blood smear.

Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

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130 Sodium

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

19 Sodium/Potassium

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

131 Sorbitol Dehydrogenase (SDH)

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: This test is included in the equine chemistry screen and all panels that include equine chemistries.

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872 Special Equine Profile

CBCEquine Chemistry Screen

Specimen Requirements Chemistry: 1.0 mL Serum,SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

Total T4 Fibrinogen

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: SDH is included in Equine Chemistry Screen.

1285 Special Liver Panel

Methodology: Spectrophotometry

AlbuminALP (Alkaline Phosphatase)

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

ALT (Alanine Transaminase)AST (Aspartate Transaminase)

GGT (Gamma-glutamyltransferase)Bilirubin, Total

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

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81 Special Pre-Op Panel

Hematocrit (HCT)GlucoseAlbumin

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTTPCV: 0.1 mL whole blood, LTT or 1 filled hematocrit

Reported Time Same Day

BUN (Blood Urea Nitrogen)Creatinine

ALT (Alanine Transaminase)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

1059 Specific Gravity, Urine

Methodology: Refractometry

Specimen Requirements 1.0 mL Urine, CTT/Urine Tube

Reported Time Same Day

Collection Notes None

Additional Comments: Included in a Urinalysis 159

651 T3 (Triiodothyronine)

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Mondays, Same day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Test is run every Monday.

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9 T3 Suppression I

Methodology: Spectrophotometry

Pre Total T4

Specimen Requirements 2 SST/RTT, Pre T4/Post T4, 0.3 mL Serum each

Reported Time Same Day

Post Total T4

Collection Notes Protocol: 1.  Day 1:  Collect baseline (pre) T4 sample.  Label tube "Pre" 2.  Day 1 & 2:  Administer 25 ug PO of T3 supplement (Cytobin or Cytomel) every 8 hours for 7 doses. 3.  Day 3:  Administer the last (7th) dose of T3 supplement on the morning of the third day and 2-4 hours later draw sample for a second T4 determination. Label tube "Post".Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: T3 suppression is done to CONFIRM feline hyperthyroidism. Interpretation of Post-suppression T4 concentration: Normal cats:  < 1.5 ug/dL or suppression greater than 50% of pre-T4 value.Inconclusive:  1.5 - 2.0 ug/dL Hyperthyroid:  > 2.0 ug/dL. If the post T4 sample is not adequately suppressed, it may be due to: 1) the cat did not receive an adequate amount of T3, or 2) the cat is hyperthyroid. Measurement of T3 concentration in the pre and post suppression samples (Test 1009 - T3 Suppression II) can be useful in verifying that the patient received the hormone supplementation.

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1009 T3 Suppression II

Methodology: Chemiluminescent Immunoassay /Spectrophotometry

Pre and Post T4

Specimen Requirements Pre and Post required, 0.5 mL (minimum 0.3mL) serum each.

Reported Time Mondays, Same Day

Pre and Post T3

Collection Notes Protocol: 1. Day 1 - collect baseline (pre) T3 and T4. Label tube "Pre" 2. Day 1&2 - administer 25ug PO of T3 supplement Cytobin or Cytomel) every 8 hours for 7 doses.3. Day 3 - administer the last (7th) dose of T3 supplement on the morning of the third day and 2-4 hours later collect sample for a second T3 and T4 determination. Label tube "Post".Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: T3 suppression is done to CONFIRM feline hyperthyroidism. Submit both samples at one time to allow analysis on the same instrument run. Interpretation of Post-suppression T4 concentration: Normal cats:  < 1.5 ug/dL or suppression greater than 50% of pre-T4 value.Inconclusive:  1.5 - 2.0 ug/dL Hyperthyroid:  > 2.0 ug/dL. If the post T4 sample is not adequately suppressed, it may be due to: 1. The cat did not receive an adequate amount of T3 2. the cat is hyperthyroid.

74 Thyroid I

Methodology: Chemiluminescent Immunoassay

Free T4

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Next day

TSH (Thyroid Stimulating Hormone)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: TSH only validated on canines, but has been used in felines.

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75 Thyroid II

Total T4

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

TSH (Thyroid Stimulating Hormone)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: TSH only validated on canines, but has been used in felines. Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

864 Thyroid III

TSH (Thyroid Stimulating Hormone)

Specimen Requirements 1.0 mL Serum, SST/RTT

Reported Time Same Day

Total T4 Free T4

Collection Notes Peak samples are drawn 4-6 hours post pill Trough samples are drawn just before the next dose.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: TSH only validated on canines, but has been used in felines.Feline T4: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

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874 Thyroid IV

Total T4

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time Same Day

Free T4

Collection Notes Peak samples are drawn 4-6 hours post pill. Trough samples are drawn just before the next dose.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

884 Thyroid Panel V

Free T4

Specimen Requirements 1.0 mL Serum, SST/RTT

Reported Time See Comment

Total T4 T3

Collection Notes Peak samples are drawn 4-6 hours post pill. Trough samples are drawn just before the next dose.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Free and Total T4 run dailyT3 run every Monday, with results out same day. Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

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49 Thyroid Screen

Total T4

Specimen Requirements 0.5 mL Serum, SST/RTT

Reported Time See Comment

T3 (Triiodothyronine)

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: T4 run same day, T3 run every Monday. Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

68 TLI, Canine

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum, SST/RTT.

Reported Time Mon/Wed/Fri, Same day

Collection Notes Patient must be fasted 12-18 hours (diabetic animals 6-8 hours).We strongly encourage that all serum samples be allowed to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Used to diagnose exocrine pancreatic insuffiency (EPI).

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689C TLI/Cobalamin/Folate (CANINE ONLY)

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 1.0 mL Serum, SST/RTT. Sample MUST be non-hemolyzed for folate testing and avoid lipemic samples.

Reported Time Mon/Wed/Fri, Same day

Collection Notes Patient must be fasted 12-18 hours (diabetic animals 6-8 hours).Cover samples with foil, vet wrap, or a paper towel to protected from light (folate and cobalamin can be light sensitive). It is recommended to separate serum and freeze the sample if transport to lab is to be delayed.We strongly encourage that all serum samples be allowed to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Tests are run in-house Monday, Wednesday, and Friday; reported out same day.Folates >24.0 ug/dL and Cobalamin >1000 are re-run on dilution and reported.

689F TLI/Cobalamin/Folate (FELINE ONLY)

Methodology: Chemiluminescent Immunoassay/RIA

Specimen Requirements 1.5 mL Serum, SST/RTT. Sample MUST be non-hemolyzed for folate testing and avoid lipemic samples.

Reported Time See Comment

Collection Notes Fast animals 12-18 hours (diabetic animals 6-8 hours) before draw.Cover samples with foil, vet wrap, or a paper towel to protected from light (folate and cobalamin can be light sensitive).We strongly encourage that all serum samples be allowed to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Cobalamin and Folate are run in-house Monday, Wednesday, and Friday; reported out same day. Folates >24.0 ug/dL and cobalamin >1000 are re-run on dilution and reported. TLI are sent to Texas GI Lab, reported in 3-7 days.

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3 Total Body Diagnostic

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urine

Reported Time Same Day

AmylaseLipase

CK (Creatine Kinase)Urinalysis

Collection Notes Please indicate method of urine collection. Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: None

35 Total Body Diagnostic w/o Urinalysis

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

LipaseAmylase

CK (Creatine Kinase)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: If you would like this panel and a Urinalysis, See Total Body Diagnostic 3

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1035 Total Body Diagnostic w/o Urinalysis, Follow Up

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

AmylaseLipase

CK (Creatine Kinase)

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

1003 Total Body Diagnostic, Follow-Up

CBCChemistry Screen

Specimen Requirements Chemistry: SST/RTT Minimum: 0.5 mL serum CBC: 2 air dried slides, 1.0mL LTT Urinalysis: 5mL urine

Reported Time Same Day

AmylaseLipase

CK (Creatine Kinase)Urinalysis

Collection Notes Please indicate method of urine collection.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

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132 Total Protein

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

244 Total T4

Methodology: Spectrophotometry

Specimen Requirements 0.5 mL Serum, SST/RTT. Minimum: 0.2 mL Serum.

Reported Time Same Day

Collection Notes For dogs, peak samples are drawn 4-6 hours post pill Trough samples are drawn just before the next dose.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

1237 Total T4, Add-On

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Peak samples are drawn 4-6 hours post pill Trough samples are drawn just before the next dose.Please allow sample to clot (up to 20 minutes) and then spin down sample. If using a RTT, remove serum and put into a fresh RTT/CTT. Avoid lipemia and hemolysis.

Additional Comments: Felines only: In the geriatric cat with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted. Available as an add-on to an existing panel (within 7 days). Previous case ID is required to activate the discounted price.

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6971 TRH Stimulation Test

Methodology: Chemiluminescent Immunoassay

ACTH, Endo Pre

Specimen Requirements 0.5 mL EDTA Plasma for Pre and Post samples, Separated and Frozen, CTT

Reported Time Mon/Wed/Fri, Same day

ACTH, Endo Post

Collection Notes Procedure:HORSES CAN BE FASTED OR MAY HAVE BEEN RECENTLY FED HAY, BUT NOT GRAIN. DO NOT PERFORM IMMEDIATELY AFTER AN ORAL SUGAR TEST.1. Collect 0 hour (PRE) TRH sample in LTT. Spin and separate plasma into a plastic transport tube within 1 hour of collection. Recommended to freeze immediately. Clearly label as 0 hour / PRE-sample.2.Inject 1 mg TRH (Tyrotropin Releasing Hormone) intravenously (IV) for horses >250 kg; inject 0.5 mg TRH IV for horses and ponies <250 kg. TRH can be obtained from compounding pharmacies.The online pharmacy Wedgewood sells TRH under the brand name Protirelin.3. At exactly 10 minutes after administration of TRH, collect second sample in LTT. Spin and separate plasma into a plastic transport tube within 1 hour of collection. Recommended to freeze immediately. Clearly label tube 10 minutes /POST-sample.

Additional Comments: Endogenous ACTH is tested on both samples. Recommended to perform test mid-November to mid-July since seasonally adjusted reference intervals have not yet been established. This is currently the recommended test for early detection of equine Pituitary Pars Intermedia Dysfunction (PPID) if a horse has clinical signs, and endogenous ACTH is inconclusive/negative.Transient yawning, coughing, mouth chomping and display flehmen response can follow administration of TRH. These side effects will subside within a few minutes.

144 Triglycerides

Methodology: Spectrophotometry

Specimen Requirements 0.5 mL Serum SST/RTT.

Reported Time Same Day

Collection Notes Patient should be fasted 12 hrs prior.Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: For triglycerides on body cavity fluid, see Body Cavity Fluid Triglycerides 2144

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1213 Tritrichomonas Fecal

Methodology: Culture

Specimen Requirements 0.05 grams Fecal in InPouch TF or Fecal tube. The sample for this test should NOT be refrigerated or put in a cooler.

Reported Time 24 hr preliminary report Final at 12 Days

Collection Notes Please submit your sample in a zip-lock bag labelled "Do Not Refrigerate". Cold temperatures will destroy the sample for testing.Pouch Procedure: 1. Pull open wired end-tabs, tearing open the pouch at the notch. 2. Open pouch sufficiently to admit a wooden applicator stick by pulling the closure tape's middle tabs apart.3. Pick-up approximately 0.05 grams, the size of a small peppercorn, or fresh feces on applicator stick(s).4.Insert applicator stick(s) with sample into the liquid of the system's upper chamber. 5. Remove specimen from stick by rubbing stick with fingers through the walls of the pouch. 6. Seal the pouch and store at room temperature in a dark place until submission to laboratory for interpretation.

Additional Comments: InPouch TF is available from Phoenix Laboratory as a CHARGEABLE supply.

248 TSH (Thyroid Stimulation Hormone)

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum, SST/RTT. Minimum: 0.2 mL Serum.

Reported Time Same Day

Collection Notes None

Additional Comments: Run on canines (for confirmation of hypothyroidism) and felines (for confirmation of hyperthyroidism.)

TSH only validated on canines, but has been used in felines.

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2481 TSH, Add-On

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Serum, SST/RTT. Minimum: 0.2 mL Serum.

Reported Time Same Day

Collection Notes None

Additional Comments: Run on canines (for confirmation of hypothyroidism) and felines (for confirmation of hyperthyroidism.)TSH only validated on canines, but has been used in felines.

Available as an add-on to an existing panel (within 7 days). Previous case ID is required to activate the discounted price.

133 Uric Acid

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Serum, SST/RTT

Reported Time Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: None

682 Uric Acid, Urine

Methodology: Spectrophotometry

Specimen Requirements 0.5 mL Urine, Urine Tube/CTT. If sample is bloody or very cloudy/cellular, submit a larger volume (1.0 mL minimum) as the sample will need to be centrifuged.

Reported Time Same Day

Collection Notes None

Additional Comments: None

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683 Uric Acid/Creat Ratio

Methodology: Spectrophotometry

Urine Uric Acid

Specimen Requirements 0.5 mL Urine, Urine Tube/CTT.If sample is bloody or very cloudy/cellular, submit a larger volume (1.0 mL minimum) as the sample will need to be centrifuged.

Reported Time Same Day

Urine Creatinine Urine, Uric Acid/Creat Ratio

Collection Notes None

Additional Comments: None

159 Urinalysis

Specimen Requirements 5 mL Urine, Urine Tube/CTT

Reported Time Same Day

Collection Notes Please indicate method of collection.Submit sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Includes: Urine specific gravity run on analyzer, verified by refractometer, Chemistries (pH, Glucose, Protein, Ketones, Blood, and Bilirubin) run on Urine analyzer, and microscopic examination of sediment. Low volume samples will use dipstick readings. Low volumes may also be changed to Urine Sediment Only 155.

548 Urinalysis w/ C&S

Urinalysis

Specimen Requirements 5 mL Urine, Urine Tube/CTT. For the most accurate results 1 mL urine in sterile container should be submitted in addition to 5 mL urine.

Reported Time See Comment

Urine Culture

Collection Notes Please indicate method of urine collection.Do not submit syringes with needles, they are not accepted. Whenever possible, urine should be collected by cystocentesis. When this is not possible, free-catch urine into sterile container.Submit sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Turn around time is same day for the UA and 36 - 72 hours for Culture. Culture includes sensitivity panels for 2 pathogens. Additional sensitivities can be requested for an additional charge. No growth cultures are finalized after 48 hours of incubation. Urine collected on a swab or thio will not allow a colony count to be provided.

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552 Urinalysis w/ Culture, Follow-up

Urinalysis

Specimen Requirements 5 mL Urine, Urine Tube/CTT. For the most accurate results 1 mL urine in sterile container should be submitted in addition to 5 mL urine.

Reported Time See Comments

Urine Culture

Collection Notes Please indicate method of urine collection.Do not submit syringes with needles, they are not accepted. Whenever possible, urine should be collected by cystocentesis. When this is not possible, free-catch urine into sterile container.Submit sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Please include all antibiotic history with urine culture.Turn around time is same day for the UA and 36 - 72 hours for Culture. Culture includes sensitivity panels for 2 pathogens. Additional sensitivities can be requested for an additional charge. No growth cultures are finalized after 48 hours of incubation. Urine collected on a swab or thio will not allow a colony count to be provided.

Submit within 30 days of original test. Previous Case ID is required to activate the discounted price

169 Urinalysis,  Add-On

Specimen Requirements 5 mL Urine, Urine Tube/CTT

Reported Time Same Day

Collection Notes Please indicate method of collection.Submit sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Includes: Urine chemistries and specific gravity run on urine analyzer (USP verified by refractometer if needed) and microscopic examination of sediment. If low volume, chemistries read by dipstick.

Available as an add-on to an existing panel( within 7 Days). Previous case ID is required to activate the discounted price.

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1159 Urinalysis, Follow-Up

Specimen Requirements 5.0 mL Urine, CTT/Urine Tube. Minimum: 2.5 mL

Reported Time Same Day

Collection Notes Please indicate method of collection of test request form.Submit sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

234 Urine Cortisol/Creatinine Ratio

Urine Cortisol

Specimen Requirements 0.5 mL Urine, Urine Tube/CTTIf sample is bloody or very cloudy/cellular, submit a larger volume (1.0 mL minimum) as the sample will need to be centrifuged.

Reported Time Same Day

Urine Creatinine Ucor/Ucreat Ratio

Collection Notes If the patient has been on steroids for >14 days, discontinue for 2 weeks prior to testing. If <14 days, discontinue for 48 hours prior to testing.

Additional Comments: INTERPRETATION:An elevated urine cortisol/creatinine ratio (> 20) provides additional support for canine hyperadrenocorticism but is not specific for that condition. Many disorders cause an increased result. A normal result (<20) makes hyperadrenocorticism very unlikely. Further testing (low dose Dexamethasone Suppression Test and/or ACTH Stimulation Test) is advised to establish a diagnosis of hyperadrenocorticism.

*Note that both cortisol and creatinine have been converted to moles to get this ratio.

196 Urine Creatinine, Phosphorus, Calcium

Methodology: Spectrophotometry

Specimen Requirements 0.35 mL Urine, Urine Tube/CTT. If sample is bloody or very cloudy/cellular, submit a larger volume (0.5 mL minimum) as the sample will need to be centrifuged.

Reported Time Same Day

Collection Notes None

Additional Comments: None

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549 Urine Culture & Sensitivity, Add-On

Methodology: Culture

Specimen Requirements 0.5 mL Urine, Urine Tube/CTT.

Reported Time 36 - 72 hours

Collection Notes Whenever possible, urine should be collected by cystocentesis. If not possible, collect catheterized or free catch urine sample into sterile container. Refrigerate urine sample after collection.

Additional Comments: Colony counts are not possible if a plate, broth, or swab is submitted. Culture includes sensitivities on up to 2 isolates. Cultures are quantitated and reported in CFU/mL. No growth cultures are finalized after 48 hours of incubation.

Available as an add-on to an existing panel only (within 7 days). Previous case ID is required to activate the discounted price.

551 Urine Culture, Follow-up

Methodology: Culture

Specimen Requirements 1.0 mL Urine, CTTCollected via cystocentesis preferred

Reported Time 36-72 hours

Collection Notes Whenever possible, urine should be collected by cystocentesis. If not possible, collect catheterized or free catch urine sample into sterile container. Refrigerate urine sample after collection.

Additional Comments: Please include all antibiotic history with urine culture.Colony counts are not possible if a plate, broth, or swab is submitted. Culture includes sensitivities on up to 2 isolates. Cultures are quantitated and reported in CFU/mL. No growth cultures are finalized after 48 hours of incubation.

Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.To include with a urinalysis, refer to Urinalysis with Culture, Follow-up 552.

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114 Urine Fractional Excretion

Methodology: Spectrophotometry

SodiumPotassiumChlorideCalciumPhosphorus

Specimen Requirements 0.5 mL Each, Serum and Urine, SST/RTT and Urine Tube/CTT. If urine sample is bloody or very cloudy/cellular, submit a larger volume (1.0 mL minimum) as the sample will need to be centrifuged.

Reported Time Same Day

GGT (Gamma-glutamyltransferase)CreatinineUrine SodiumUrine PotassiumUrine Chloride

Urine CalciumUrine PhosphorusUrine GGT (Gamma-glutamyltransferase)Urine Creatinine

Collection Notes Both Serum and Urine must be collected at the same time. Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Measures sodium, potassium, chloride, calcium, phosphorus, GGT, and creatinine in both serum and urine and calculates fractional excretion.

2234 Urine Mineral Screen

Methodology: Spectrophotometry

UrinalysisUrine PotassiumUrine PhosphorusUrine Calcium

Specimen Requirements 5.0 mL Urine, Urine Tube/CTT.

Reported Time Same Day

Urine ProteinUrine SodiumUrine MagnesiumUrine Creatinine

Urine ChlorideUrine Protein/Creat Ratio

Collection Notes Please indicate method of urine collection.Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments:

1031 Urine Protein Electrophoresis

Methodology: Agarose Gel Electrophoresis

Specimen Requirements 5 mL Urine, CTT/Urine Tube

Reported Time Monday/Thursday

Collection Notes None

Additional Comments: Please submit one day before testing to allow for urine concentration.

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681 Urine Protein/Creatinine Ratio

Methodology: Spectrophotometry

Urine Protein

Specimen Requirements 0.5 mL Urine, Urine Tube/CTTIf sample is bloody or very cloudy/cellular, submit a larger volume (1.0 mL minimum) as the sample will need to be centrifuged. Hemorrhage or inflammation will affect testing.

Reported Time Same Day

Urine Creatinine Urine Protein/Creatinine Ratio

Collection Notes Preferably collected by cystocentesis

Additional Comments: Values > 1.0 are suggestive of glomerular proteinuria in dogs and cats.

155 Urine Sediment Only

Methodology: Centrifugation/Microscopic

Specimen Requirements 5 mL of urine OR sediment from 5 mL of fresh urine in Urine Tube/CTT

Reported Time Same Day

Collection Notes Submit urine sample in a urine tube or sterile, clear top vacutainer; some RTT may cause crystalline debris due to silica.

Additional Comments: Due to poor cellular preservation, this test is not preferred for diagnosis of malignancy; recommend submission of freshly made urine sediment smears and reconstituted urine sediment with saline in a LTT for cytology.

Please contact our client services for optimum sample handling.

183 Urine, Calcium

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Urine, Urine Tube/CTT. If sample is bloody or very cloudy/cellular, submit a larger volume (0.5 mL minimum) as the sample will need to be centrifuged.

Reported Time Same Day

Collection Notes None

Additional Comments: Consider ordering a Urine Fractional Excretion panel 114 as clinically indicated.

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189 Urine, Chloride

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Urine, Urine Tube/CTT. If sample is bloody or very cloudy/cellular, submit a larger volume (0.5 mL minimum) as the sample will need to be centrifuged.

Reported Time Same Day

Collection Notes None

Additional Comments: Consider ordering a Urine Fractional Excretion panel 114 as clinically indicated.

233 Urine, Cortisol

Methodology: Chemiluminescent Immunoassay

Specimen Requirements 0.5 mL Urine, Urine Tube/CTTIf sample is bloody or very cloudy/cellular, submit a larger volume (0.5 mL minimum) as the sample will need to be centrifuged.

Reported Time Same Day

Collection Notes None

Additional Comments: None

184 Urine, Creatinine

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Urine, Urine Tube/CTT. If sample is bloody or very cloudy/cellular, submit a larger volume (0.5 mL minimum) as the sample will need to be centrifuged.

Reported Time Same Day

Collection Notes None

Additional Comments: Please consider a Urine Protein/Creatinine Ratio 681.

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879 Urine, Electrolytes

Methodology: Spectrophotometry

Urine Potassium

Specimen Requirements 0.5 mL Urine, Urine Tube/CTTIf sample is bloody or very cloudy/cellular, submit a larger volume (1.0 mL minimum) as the sample will need to be centrifuged.

Reported Time Same Day

Urine Sodium Urine Chloride

Collection Notes None

Additional Comments: Consider ordering a Urine Fractional Excretion panel 114 as clinically indicated.

192 Urine, GGT

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Urine, Urine Tube/CTT. If sample is bloody or very cloudy/cellular, submit a larger volume (0.5 mL minimum) as the sample will need to be centrifuged.

Reported Time Same Day

Collection Notes None

Additional Comments: Consider ordering a Urine Fractional Excretion panel 114 as clinically indicated.

182 Urine, Phosphorus

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Urine, Urine Tube/CTT. If sample is bloody or very cloudy/cellular, submit a larger volume (0.5 mL minimum) as the sample will need to be centrifuged.

Reported Time Same Day

Collection Notes None

Additional Comments: Consider ordering a Urine Fractional Excretion panel 114 as clinically indicated.

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180 Urine, Potassium

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Urine, Urine Tube/CTT. If sample is bloody or very cloudy/cellular, submit a larger volume (0.5 mL minimum) as the sample will need to be centrifuged.

Reported Time Same Day

Collection Notes None

Additional Comments: Consider ordering a Urine Fractional Excretion panel 114 as clinically indicated.

185 Urine, Protein

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Urine, Urine Tube/CTT. If sample is bloody or very cloudy/cellular, submit a larger volume (0.5 mL minimum) as the sample will need to be centrifuged.

Reported Time Same Day

Collection Notes None

Additional Comments: Please consider a Urine Protein/Creatinine Ratio 681.

186 Urine, Sodium

Methodology: Spectrophotometry

Specimen Requirements 0.25 mL Urine, Urine Tube/CTT. If sample is bloody or very cloudy/cellular, submit a larger volume (0.5 mL minimum) as the sample will need to be centrifuged.

Reported Time Same Day

Collection Notes None

Additional Comments: Consider ordering a Urine Fractional Excretion panel 114 as clinically indicated.

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181 Urine, Sodium and Potassium

Methodology: Spectrophotometry

Urine Sodium

Specimen Requirements 0.25 mL Urine, Urine Tube/CTT. If sample is bloody or very cloudy/cellular, submit a larger volume (0.5 mL minimum) as the sample will need to be centrifuged.

Reported Time Same Day

Urine Potassium

Collection Notes None

Additional Comments: Consider ordering a Urine Fractional Excretion panel 114 as clinically indicated.

862 Uterine Screen, Equine

Uterine Culture

Specimen Requirements Saline Flush in sterile container (CTT) is the preferred sample. (Allows for cytospin prep which provides the best cell interpretation and can be used for both cytology and culture). OR 2 transport swabs AND freshly made slides are OK

Reported Time See Comment

Uterine Cytology

Collection Notes Slides are ideally prepared immediately after collection. Prepare the cytology sample by GENTLY ROLLING (NOT SMEARING) the swab over the slide.This technique will deliver optimal cellular material for review.  Thoroughly dry the smears. DO NOT put slides into the slide holder until completely dry or all the cells will rupture. Do not refrigerate slides.

Please note: Slides prepared at lab from swabs have variable quality. Smears prepared on non-sterile slides may result in contamination of the culture.

Additional Comments: Cytology reported the same day (Monday - Saturday on Days). Culture report time 36 - 72 hours.

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387 Vector/Tick Borne Disease Screen

Methodology: Lateral Flow

Specimen Requirements 0.5 mL Serum, SST/RTT. Plasma or whole blood (EDTA or heparinized) may be used.

Reported Time Daily AM - Same Day

Collection Notes Please allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot. This limits hemolysis and other artifact changes which can affect the results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Test includes: Ehrlichia canis, Ehrlichia ewirgii, Ehrlichia chaffeensis, Anaplasma phagocytophilum, Anaplasma platys, Borrelia burgdorferi (Lyme Disease), and Heartworm (D. immitis).

Test methodology unable to differentiate between Anaplasma phagocytophilum and Anaplasma platys. Also unable to differentiate between Ehrlichia canis, Ehrlichia chaffeensis, and Ehrlichia ewingii.

Also see Canine Tick-borne Disease panel, SO666 if screening for Rocky Mountain Spotted Fever is desired.

932 WBC Estimate and Diff

Methodology: Manual

Specimen Requirements 3 Air dried peripheral blood smears

Reported Time Same Day

Collection Notes Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Quality slides are essential for accurate evaluation.

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1711 Wellness Screen II

CBCChemistry ScreenUrinalysis

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urine

Reported Time Same Day

AmylaseLipase

CK (Creatine Kinase)Total T4

Collection Notes Please indicate method of urine collection. Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: To request this panel, but submit the urinalysis portion later, see Wellness Screen II w/ Urine to Follow 1711NU

1721 Wellness Screen II w/ SDMA

CBCChemistry Screen Urinalysis

Specimen Requirements Chemistry: 1.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urine

Reported Time See comment

AmylaseLipaseSDMA

CK (Creatine Kinase)Total T4

Collection Notes Please indicate method of collection.Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: CBC, Chemistries, and urinalysis run same day, SDMA Results Next Day. See SDMA SO513 for more details about test.

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1711NU Wellness Screen II w/ Urine to Follow

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

AmylaseLipase

CK (Creatine Kinase)Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: When submitting the urine, please request Urine, No Charge 179 on a separate test request form, and include the previous case ID.

1712 Wellness Screen III

CBCChemistry ScreenFree T4

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same day

AmylaseLipase

CK (Creatine Kinase)Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Panel includes Wellness Screen I and Free T4. Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

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1713 Wellness Screen IV

CBCChemistry ScreenUrinalysis

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smearsUrinalysis: 5 mL fresh urine

Reported Time Same day

AmylaseLipaseFree T4

CK (Creatine Kinase)Total T4

Collection Notes Please indicate method of urine collection. Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted.

15 Wellness/Geriatric Panel

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time Same Day

AmylaseLipase

CK (Creatine Kinase)Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: None

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1722 Wellness/Geriatric Panel w/ SDMA

CBCChemistry ScreenSDMA

Specimen Requirements Chemistry: 1.5 mL Serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time See comment

AmylaseLipase

CK (Creatine Kinase)Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: CBC and Chemistries run same day, SDMA results next day. See SDMA SO513 for more details about test.

1160 Wellness/Geriatric Panel w/Triglycerides

CBCChemistry ScreenTriglycerides

Specimen Requirements Chemistry: 0.5 mL serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT, air dried peripheral blood smears

Reported Time Same Day

AmylaseLipase

CK (Creatine Kinase)Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: None

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1015 Wellness/Geriatric Panel, Follow-Up

CBCChemistry Screen

Specimen Requirements Chemistry: 0.5 mL serum, SST/RTT, 1.0 mL GTTCBC: 1.0 mL LTT, air dried peripheral blood smears

Reported Time Same Day

AmylaseLipase

CK (Creatine Kinase)Total T4

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Submit within 30 days of original test. Previous Case ID is required to activate the discounted price.

881 Wellness/Geriatric Profile w/ Feline Viral I

CBCChemistry ScreenAmylase

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time See Comment

LipaseCK (Creatine Kinase)Total T4

FeLV (ELISA)FIV (IFA)

Test Alias: Feline Panel 881

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.Thoroughly dry slides before inserting into slide holder and do not refrigerate slides.

Additional Comments: Chemistry, CBC, and FeLV run same day, FIV run daily, AM. Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted. Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

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858 Wellness/Geriatric Profile w/ Feline Viral II

CBCChemistry ScreenAmylase

Specimen Requirements Chemistry: 1.0 mL Serum, SST/RTT, 1.0 mL GTT CBC: 1.0 mL LTT and air dried peripheral blood smears

Reported Time See Comments

LipaseCK (Creatine Kinase)Total T4

FeLV (ELISA)FIV (IFA)FCoV (FIP) Screen (IFA)

Test Alias: Total Feline Panel

Collection Notes Prolonged contact with cells can dramatically affect glucose values.For accurate glucose assessment, allow all serum samples (RTT/CTT/SST) to clot and then centrifuge the sample within 20-30 minutes of collection, separating serum completely from clot (or also submit a GTT). This also limits hemolysis which can affect other results.If using a RTT/CTT, remove serum and put into a fresh RTT/CTT.Avoid lipemia and hemolysis.

Additional Comments: Chemistry, CBC, and FeLV run same day, FIV/FIP run daily, AM. Felines only: In geriatric cats with T4 values >2.5 ug/dL, consider work up for hyperthyroidism if clinically warranted. Please see the current retroviral testing recommendations from the American Association of Feline Practitioners (AAFP, www.catvets.com)

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