tg (hsp70:mcherry; cmv:naos3’utr:gfp)
DESCRIPTION
Tg (hsp70:mcherry; CMV:naos3’UTR:GFP). 8 hrs. 16 hrs. Express EGFP in germ cell. 24 hrs. Tg (dkk1b-2Ki1). Express EGFP in pharyngeal arch and otic vesicle. TgIBStrap1. Day 1 . Day 2 . Expressed in lateral line. Tg (gnrh3-GFP-LacZ). - PowerPoint PPT PresentationTRANSCRIPT
Tg(hsp70:mcherry; CMV:naos3’UTR:GFP)24
hrs
8
hrs
16 h
rs
Express EGFP in germ cell
Tg(dkk1b-2Ki1)
Express EGFP in pharyngeal arch and otic vesicle.
TgIBStrap1D
ay 2
D
ay 1
Expressed in lateral line
Tg(gnrh3-GFP-LacZ)
Expression and migration of GFP- and LacZ-expressing
cells. A, B, F, G, ventral view; C-E, dorsal view. A, C, F, G,
GFP cells; D, E, LacZ-expressing cells. A, GFP expression at
the olfactory placode (OP). B-D, GFP or LacZ cells begin to
migrate into telencephalon (Tel) at 3-8 d (days). E, lacZ
cells and axons were detected in brain at 16dpf. F-G, At 1
month (m), fluorescent axonal extensions are detected at
OB, Tel and POA. Red arrows indicate cell bodies, yellow
arrows indicate axons. POA, preoptic area.
2k1k
1kE1
2k:GFP; 1d 2k:GFP; 2d 2k:GFP; 3d
1k:GFP; 1d 1k:GFP; 2d 1k:GFP; 3d
1kE1:GFP; 1d 1kE1:GFP; 2d 1kE1:GFP; 3d
C D E
F G H
I J K
4k
4k:GFP; 1d 4k:GFP; 2d 4k:GFP; 3d
L M N
Tg-dkk1b-1k ,1kE1, 2k, 4k
Expressed in pharyngeal arches, otic vesicle, whole body(4k)
Tg(zpopdc1:GFP)
Tg(J11:EGFP)