th th june 18 to 20 , 2009 18 au 20 juin 2009 · 2014-06-13 · 18 au 20 juin 2009 june 18 to 20 ,...
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C Y T O M E T R Y S Y M P O S I U M
nd
2 ième SYMPOSIUM DE CYTOMÉTRIE
18 au 20 Juin 2009
th th
June 18 to 20 , 2009
McGill University / Université McGillNew Residence HallMontréal, Québec
www.cytometry.ca©Claudio Calligaris
Thanks you to Academic Core Facilities for support -
Merci aux Plateformes de Service Académiques pour leur soutien
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Welcome to the Canadian Cytometry Assiociation’s 2nd Cytometry Symposium. Visit the CCA’s website (www.cytometry.ca) to find resources on Microscopy and Cytometry and to find out more about the CCA. We wish you an interesting and productive meeting. Bienvenue au 2nd Symposium de Cytométrie organisé par l’Association Canadienne de Cytométrie. Visitez le site Web de l’ACC (www.cytometry.ca) pour trouver des informations sur la microscopie et la cytométrie et pour en savoir plus sur l’ACC. Nous vous souhaitons une conférence intéressante et productive.
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The organizing committee / Le comité organisateur
Laurence Lejeune President CCA – Présidente de l’ACC Manager - Cytometry Facility, CHUM Research Centre-, St-Luc Hospital Responsable - Service de Cytométrie, Centre de Recherche du CHUM, Hôpital Saint Luc
Claire Brown, PhD Scientific Officer CCA –Officier Scientifique ACC Director - Imaging Facility, Life Sciences Complex, McGill University Directrice - Service d’Imagerie, Complexe des Sciences de la Vie, Université McGill
Aleks Spurmanis Microscopy Specialist - Imaging Facility, Life Sciences Complex, McGill University Spécialiste de Microscopie - Service d’Imagerie, Complexe des Sciences de la Vie, Un. McGill
Guillaume Lesage, PhD Administrator - Cell Imaging and Analysis Network, Dept. Biology, McGill University Administrateur - Cell Imaging Analysis Network, Dépt. Biologie, Université McGill
Judith Lacoste, PhD Communication Officer CCA – Officier de communication ACC Microscopy Specialist - CIAN Dept. Biology, McGill University and MIA Cellavie Inc. Spécialiste de Microscopie - CIAN, Dépt. Biologie, Université McGill et MIA Cellavie Inc.
Gabriel Lapointe PhD Candidate - Dept. Biochemistry, University of Montreal Étudiant au Doctorat - Dépt. Biochimie, Université de Montréal
Marie-Hélène Lacombe Treasurer CCA - Trésorière ACC Cytometry Service - Dept. Microbiology and Immunology, McGill University Service de Cytométrie - Dépt. Microbiologie et Immunologie, Université McGill
Christian Young Manager - Flow Cytometry Facility– Lady Davis Institute Responsable du Service de Cytométrie – Institut Lady Davis
Danièle Gagné Communication Officer CCA – Officier de communication ACC Manager - Cytometry Facility, IRIC, University of Montreal Responsable - Service de Cytométrie, IRIC, Université de Montréal
Ken McDonald Flow Cytometry Facility Manager – Life Science Complex, McGill University Responsible Service de Cytométrie – Complexe des Sciences de la vie, Université McGill
Milena Crosato Administrative Coordinator - Segal Cancer Centre research laboratories Coordonnatrice Administrative - Laboratoires de Recherche du Centre du cancer Segal .
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TABLE OF CONTENTS
2009 Cytometry Symposium at a Glance......................................................................4
Agenda .......................................................................................................................5 Thursday, June 18, 2009 ........................................................................................................................................5 Friday, June 19, 2009...............................................................................................................................................5 Saturday, June 20, 2009..........................................................................................................................................7
Room Organization .....................................................................................................9
Abstracts – Oral Presentations ..................................................................................10
Abstracts – Poster Presentations...............................................................................23
Symposium Attendees ..............................................................................................31
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2009 CYTOMETRY SYMPOSIUM AT A GLANCE
Thursday June 18 Friday June 19 Saturday June 20
KEYNOTE LECTURE PLENARY SESSIONS TUTORIAL SESSIONS
08:00
Registration Registration
08:00
09:00
09:00
Introduction to Cytometry Session1: Sample preparation
10:00
Break- Posters - Exhibits Coffee break – Posters – Exhibits
10:00
11:00
11:00
Automated Imaging
12:00
Registration for CFM meeting
Session 2A: Image Collection and Analysis
Session 2B: Flow Cytometry Data Analysis 12:00
13:00 13:00 Lunch – Posters - Exhibits
Lunch – Posters – Exhibits 14:00 14:00
15:00
Core Facility manager meeting
Multiparameter flow cytometry
15:00
Break – Posters - Exhibits
Session 3A: Multi-color Applications
Session 3B: Functional Assays
16:00 Light Refreshments
Closing remarks
16:00
17:00
17:00
18:00 Registration
Watching Biology as it Happens
18:00
19:00 KEYNOTE ADDRESS
N. Labrecque
19:00
20:00 Opening Wine and Cheese
20:00
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AGENDA
Thursday, June 18, 2009
13:00 Core Facility Manager meeting
16:00 Light Refreshments (for Core Facility Manager Meeting attendees) 17:00-19:00 Registration
19:00 KA KEYNOTE ADDRESS
Nathalie Labrecque (Hôpital Maisonneuve-Rosemont, Université de Montréal) Life of a T cell: from precursor to memory
20:00 Opening Wine and Cheese
Friday, June 19, 2009
08:00-17:00 Registration 09:00 Welcome address
Laurence Lejeune, President of the Canadian Cytometry Association
09:10-10:35 Plenary Session 1: Introduction to Cytometry
Sponsored by Media Cybernetics
09:10 O1 Judith Lacoste (CIAN, McGill University and MIA Cellavie)
Introduction to Cytometry
09:30 O2 Laurence Lejeune (Hôpital St-Luc, Université de Montréal) Introduction to Flow Cytometry
09:50 O3 Claire Brown (Life Sciences Complex Imaging Facility, McGill University) Introduction to Light Microscopy
10:10 O4 Arnaud Bonnefoy (Montreal Heart Institute and INSERM U753) Cell Membrane Liquid Order Profiling with Di-4-ANEPPDHQ. Applications to Drug Monitoring, Cell
Activation and Cell Sub-Population Detection
10:35 Coffee Break
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11:00 – 12:30 Plenary Session 2: Automated Imaging: Bridging the Gap between Flow and Microscopy
11:00 O5 Claire Brown (Life Sciences Complex Imaging Facility, McGill University)
Introduction to High Content Screening (HCS)
11:15 O6 Eric Lebel (Olympus Canada Inc.) The New Olympus FV10i Confocal System
11:40 O7 Steve Luke (MDS Analytical Technologies)
Introducing the IsoCyte, the High Speed Laser Scanning Cytometer from MDS Analytical
Technologies
12:05 O8 Mohamed Kodiha (Dept. Physiology, McGill University) Quantitative Immunofluorescence: A novel Tool to Analyze the Subcellular Distribution of Transport
Receptors and Signaling Molecules.
12:30 Lunch (provided thanks to BD Biosciences)
Poster session Vendors exhibit
14:00 CCA General Assembly / Assemblée Générale de l’ACC
14:30-16:10 Plenary Session 3: Development and Future of Multiparameter Flow Cytometry
14:30 O9 Raphael Schneider (Hôpital Notre-Dame, Université de Montréal)
Enhanced Levels of Interleukin-15 in Multiple Sclerosis Contribute to Augmented CD8 T Cell Effector
Functions
14:55 O10 Patricia Ndumbi (Medicine, McGill University)
T-Cell Homeostasis: Implications in HIV Pathogenesis
15:20 O11 Peter Darlington (Montreal Neurological Institute, McGill University) Cytometry with Combinatorial Intracellular Cytokine Staining Reveals Distinct Regulatory Effects of
Natural Killer Cells on Pro-Inflammatory T Cell Responses.
15:45 O12 Greg Finak (Institut de Recherches Cliniques de Montréal) Merging Mixture Model Components for Improved Cell Population Identification in High Throughput
Flow Cytometry Data
16:10 Coffee Break
16:40-18:45 Plenary Session 4: Watching Biology as it Happens
Sponsored by MIA Cellavie Inc.
16:40 O13 Aleks Spurmanis (Life Sciences Complex Imaging Facility, McGill University)
Fundamentals of 3D Fluorescence Microscopy
17:05 O14 Judith Lacoste (CIAN, McGill University and MIA Cellavie Inc.) Live-Cell Microscopy – Tips and Tools
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17:30 O15 Jonas Dorn (IRIC, Université de Montréal) Analysis of CENP-A Replenishment in Early G1
17:55 O16 Haouaria Balghi (McGill University)
Measuring and Visualizing Calcium in Live Cells
18:20 O17 Brian Northan (Media Cybernetics)
Digital Deconvolution of 3 Dimensional Biological Images
Saturday, June 20, 2009
08:00-12:00 Registration
09:00-10:30 Tutorial Session 1: Sample Preparation
09:00 O18 Aleks Spurmanis (Life Sciences Complex Imaging Facility, McGill University)
Essentials of Sample Preparation for Microscopy
09:20 O19 Laurence Lejeune (Hôpital St-Luc, Université de Montréal) Essentials of Sample Preparation for Flow Cytometry
09:40 O20 Gayle Buller (Invitrogen Corp.) Fluorescence, Fluorochromes, and Functional Reagents
10:30 Coffee Break
11:00-12:30 Tutorial Session 2A: Image Collection and Analysis
Sponsored by Photometrics
11:00 O21 Claire Brown (Life Sciences Complex Imaging Facility, McGill University)
What Is an Image?
11:30 O22 Mic Chaudoir (Photometrics) How to Evolve your Imaging
12:00 O23 Gabriel Lapointe (Dépt de Biochimie, Université de Montréal) Image Processing and Analysis
11:00-12:30 Tutorial Session 2B: Flow Cytometry Data Analysis
11:00 024 Bastian R Angermann (Université de Montréal)
Perspectives on Flow Informatics
11:25 O25 David McFarlane (Beckman Coulter Inc.) Kaluza Analysis Software – A New Approach to Flow Cytometry Data Analysis
12:05 O26 Aaron Hart (Tree Star Inc.) Flow Data Analysis, Methods and Techniques
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12:30 Lunch Poster sessions Vendors exhibit
14:30-16:30 Tutorial Session 3A: Multi-color Applications
Sponsored by Carl Zeiss Canada Inc.
14:30 O27 Laurence Lejeune (Hôpital St-Luc, Université de Montréal)
Introduction to Multi-Color Flow Cytometry
15:00 O28 Alexandre Brunet (BD-Biosciences) Introducing the Newest Member of the Family. A Compact Powerful Alternative for your Flow
Cytometry Needs.
15:20 O29 Judith Lacoste (CIAN, McGill University and MIA Cellavie)
Introduction to Co-Localization
16:00 O30 Ian Bates (Carl Zeiss Canada Inc.) Linear Un-Mixing and Spectral Imaging
14:30-16:30 Tutorial Session 3B: Functional Assays
14:30 O31 Dwayne Dexter (Cyntellect Inc.)
High-Throughput In Situ Purification Of Living Cells Using Targeted Laser Ablation
15:00 O32 Sesha Tekur (ThermoFisher Scientific) Utility of High Content Imaging Assays to Address Cell Cycle
15:30 O33 Rich DeMarco (Amnis Corp.) Functional Assays Using ImageStream Cytometry: Image-Based Measurement of Apoptosis, Cell
Cycle and Nuclear Translocation in Flow
16:00 O34 Laurence Lejeune (Hôpital St-Luc, Université de Montréal) Flow Cytometry Approaches to Cell Cycle Analysis, Apoptosis and Cell Proliferation
16:30 Closing remarks
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ROOM ORGANIZATION
Keynote AddressPlenary SessionsTutorial Sessions A
Wine and cheeseSponsors Exhibit
Posters
Tutorial Sessions B
Keynote AddressPlenary SessionsTutorial Sessions A
Wine and cheeseSponsors Exhibit
Posters
Tutorial Sessions B
Keynote AddressPlenary SessionsTutorial Sessions A
Wine and cheeseSponsors Exhibit
Posters
Tutorial Sessions B