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STUDY OF PATHOGENESIS OF PAIN IN COMPLEX BODY DISORDERS. Rajesh Kumar*, S. Mishra*, Sanjeev Kumar*, Ravindra Kumar*, M.M. Bajaj, Medical Physics Research Laboratory, Department of Physics and Astrophy- sics, University of Delhi, Delhi, 110 007, India. AIM OF INVESTIGATION: We have applied Laser Raman and FTIR techniques t Abs No 62

understand different pain inducing diseases (Tuberculosis, Leprosy, Burns Epilepsy and Muscular Dystrophy) and procure information about the subtle changes occurring _ at molecular level in these disorders with respect to pain perception. METHODS: We have taken 5ml. of blood samples of Tuberculosis, Leprosy, Burns, Epilepsy,

DMD and normal cases from different hospitals (Total number of clinical cases: 27,000; hard core experiments: 210 samples). After centrifugation the serum was separated and cells were washed with cold saline and then dehydrated over P2O5. Ion exchange chromatographic tech- nique was used for separation of IgG samples. The purity of so obtained samples were further tested with the help of antisera using single radial immunodiffusion technique. Infrared spectrophotometer Perkin Elmer Model 1710 was used to record spectra of prepared samples, having resolution 1 to 64 cm-I with a memory option fitted. Laser Raman spectrophotometer (Mode N-R-1000) consisting of Photomultiplier unit (R-464) was used to record the spectra of different pain inducing diseases and normals in the 300-3000 cm-I region.

RESULT: For every disease we obtained specific signals in I.R. spectra. Result of the ob- served bands of hemoproteins and immunoglobulins are summarised and compared with those of normals to get information about pain perception. Presence of specific bands, their vibra- tional energies, force constants and their correlation with pain inducing diseases in terms of frequency shifts of Raman modes are given.

CONCLUSIONS: It is concluded that bands arising due to O-H, N-H, C-C, P-O-C, O-O, C-H bonds are severely affected in case of P.T.B., whereas significant shift in strong signals is ob- served in lattice water, amide-I and metal ligands stretching regions in L.L. cases. The C-C, C-H symmetric and asymmetric CH2 and CH3 vibrations are impaired following thermal injuries particularly in the pseudomonas aeruginosa infection. In FTIR spectra, strong peak in hydro- carbon region, and disappearance of few normal bands are distinct features of neurodisorders. These ought to be associated with the pathogenesis of pain perception.

THE BEHAVIORAL MANAGEMENT OF PEDIATRIC HEADACHE: A FOLLOW-UP STUDY. W.M. Womack, *M.S. Smith*, and A.C.N. Chen, (Sponsor: Department of Psychiatry & Pediatrics, University of Washington School of Medicine. Seattle, Washington

Ms. Louisa Jcja

AIM OF INVESTIGATION: Recurrent headache is a common problem among Abs No 63 .I

children and adolescents and behavioral techniques such as relaxation i training, biofeedback, self-hypnosis, and cognitive therapy have been shown to be effect- ive treatment modalities. This report describes the status of headache activity in our study population over a period of one-six years following treatment (56 patients, 25 male, 31 female). METHODS: Mailed questionnaires which provided information about: Post-treatment status

of pain medication, current practices of behavioral techniques for headache control, per- ceived efficacy of behavioral techniques , perceived credibility of treatment approach, and over-all satisfaction with the treatment program. Ten centimeter visual analog scales were used for headache activity and attitudes about the treatment program.

RESULTS: The reduction in headache activity was statistically significant at follow-up, while the decrease in headache intensity was not. Depression scores were significantly reduced, while anxiety scores were not. Headache frequency at intake was correlated with anxiety and depression scores at follow-up. 66% of patients were still practicing be- havioral techniques for headache control. There were significant negative correlations between headache frequency and treatment credibility and satisfaction. Headache intensity was negatively correlated with current practice of behavioral techniques.

CONCLUSION: The data suggests that headache frequency is significantly decreased by be- havioral treatment and that the treatment effect persists with time. No measures other than headache activity at intake were helpful in.predicting outcome headache status. Continued practice resulted in decreased headache intensity and improved headache frequen- cy was significantly related to higher levels of treatment credibility and satisfaction.