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The DNA Bank Network Use Cases Gabriele Droege Botanic Garden and Botanical Museum Berlin-Dahlem Freie Universität Berlin

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Page 1: The DNA Bank Network Use Cases Gabriele Droege Botanic Garden and Botanical Museum Berlin-Dahlem Freie Universität Berlin

The DNA Bank NetworkUse Cases

Gabriele Droege

Botanic Garden and Botanical Museum Berlin-Dahlem

Freie Universität Berlin

Page 2: The DNA Bank Network Use Cases Gabriele Droege Botanic Garden and Botanical Museum Berlin-Dahlem Freie Universität Berlin

Botanic Garden and Botanical Museum Berlin-Dahlem

Plants, algae and protists

Coordination

Zool. Forschungsmuseum Alexander Koenig, Bonn

Invertebrates and vertebrates

Bavarian State Collection of Zoology, Munich

Invertebrates and fungi

German Collection of Microorganisms and Cell Cultures, Braunschweig

Microorganisms

DNA Bank Network

The New York Botanical Garden

Plants

Funding 2007-2011

Page 3: The DNA Bank Network Use Cases Gabriele Droege Botanic Garden and Botanical Museum Berlin-Dahlem Freie Universität Berlin

Goals

– Deposition and Long-term storage of DNA samples and vouchers

– Providing standardized access to DNA samples

– Data infrastructure for reference purposes

– Use of existing systems (e.g. GBIF, GenBank, CoL)

– Partners work independent

Page 4: The DNA Bank Network Use Cases Gabriele Droege Botanic Garden and Botanical Museum Berlin-Dahlem Freie Universität Berlin

Source material / specimens Molecular analysis data

The Reference Problem

DNA Bank Network Webportal

Page 5: The DNA Bank Network Use Cases Gabriele Droege Botanic Garden and Botanical Museum Berlin-Dahlem Freie Universität Berlin

Search & Preorder

Page 6: The DNA Bank Network Use Cases Gabriele Droege Botanic Garden and Botanical Museum Berlin-Dahlem Freie Universität Berlin

Search & Preorder

Page 7: The DNA Bank Network Use Cases Gabriele Droege Botanic Garden and Botanical Museum Berlin-Dahlem Freie Universität Berlin

Search & Preorder

Data Privacy Protection:

Customer details recorded in index database and forwarded after finishing preorder.

Shopping cart is deleted after finishing preorder.

Shopping history is not recorded in index database!

Every DNA bank receives its relevant order information only.

Page 8: The DNA Bank Network Use Cases Gabriele Droege Botanic Garden and Botanical Museum Berlin-Dahlem Freie Universität Berlin

Where are the vouchers deposited?

Both deposited @ BGBM

Page 9: The DNA Bank Network Use Cases Gabriele Droege Botanic Garden and Botanical Museum Berlin-Dahlem Freie Universität Berlin

Where are the vouchers deposited?

DNA @ BGBM

Specimen @ SI

Page 10: The DNA Bank Network Use Cases Gabriele Droege Botanic Garden and Botanical Museum Berlin-Dahlem Freie Universität Berlin

Verification of determination

Digital voucher images whenever possible

Page 11: The DNA Bank Network Use Cases Gabriele Droege Botanic Garden and Botanical Museum Berlin-Dahlem Freie Universität Berlin

Verification of determination

Digital voucher images whenever possible

Page 12: The DNA Bank Network Use Cases Gabriele Droege Botanic Garden and Botanical Museum Berlin-Dahlem Freie Universität Berlin

Verification of determination

Digital voucher images whenever possible

Page 13: The DNA Bank Network Use Cases Gabriele Droege Botanic Garden and Botanical Museum Berlin-Dahlem Freie Universität Berlin

External Vouchers

Berlin DNA bank

Where to find the data?

Page 14: The DNA Bank Network Use Cases Gabriele Droege Botanic Garden and Botanical Museum Berlin-Dahlem Freie Universität Berlin

Where are the vouchers?

No information about deposition of voucher and DNA sample available.

Requirement: Crosslinks between GenBank, GGBN and GBIF (all directions)

Page 15: The DNA Bank Network Use Cases Gabriele Droege Botanic Garden and Botanical Museum Berlin-Dahlem Freie Universität Berlin

Are there DNA samples or GenBank accessions?

No information about further DNA samples/molecular analysis.

Requirement: Crosslinks between GBIF, GGBN and GenBank (all directions)

Page 16: The DNA Bank Network Use Cases Gabriele Droege Botanic Garden and Botanical Museum Berlin-Dahlem Freie Universität Berlin

Associations

In situ voucher

DNA voucher (cultivated plant)

Tissue and Images from cultivated living plant

DNA Sample

Page 17: The DNA Bank Network Use Cases Gabriele Droege Botanic Garden and Botanical Museum Berlin-Dahlem Freie Universität Berlin

Associations

Vision:

All associated voucher data connected to DNA data

Page 18: The DNA Bank Network Use Cases Gabriele Droege Botanic Garden and Botanical Museum Berlin-Dahlem Freie Universität Berlin

Associations

Algae and sponge

Leaf miner and Plant

Algae and Plant deposited in a herbarium but what about the animals and vice versa?

Collaboration with other DNA banks

Association of data

Page 19: The DNA Bank Network Use Cases Gabriele Droege Botanic Garden and Botanical Museum Berlin-Dahlem Freie Universität Berlin

DNA parametersm

andato

ry

Page 20: The DNA Bank Network Use Cases Gabriele Droege Botanic Garden and Botanical Museum Berlin-Dahlem Freie Universität Berlin

On-demand extractions

Page 21: The DNA Bank Network Use Cases Gabriele Droege Botanic Garden and Botanical Museum Berlin-Dahlem Freie Universität Berlin

Metadata, Citation format

Page 22: The DNA Bank Network Use Cases Gabriele Droege Botanic Garden and Botanical Museum Berlin-Dahlem Freie Universität Berlin

Publications etc.

• After ordering DNA the customers are asked by DNA bank curators to inform the relevant DNA bank about publications, GenBank accessions etc. related to orderêd DNA samples and follow the citation format (see Metadata).

Task for DNA bank curators not for the platform.

Page 23: The DNA Bank Network Use Cases Gabriele Droege Botanic Garden and Botanical Museum Berlin-Dahlem Freie Universität Berlin

Citations of DNA and vouchers

Vision:

All available publications related to a single DNA or voucher connected to our data

Following the DSMZ example.

How can GGBN help?

Page 24: The DNA Bank Network Use Cases Gabriele Droege Botanic Garden and Botanical Museum Berlin-Dahlem Freie Universität Berlin

Reports

• Record every search key and resulting hits in database

• Web statistics

-> what are people looking for?-> what search key often results in “no hits”

help to find gaps

Page 25: The DNA Bank Network Use Cases Gabriele Droege Botanic Garden and Botanical Museum Berlin-Dahlem Freie Universität Berlin

Catalogue of Life

Help to find the gaps

Page 26: The DNA Bank Network Use Cases Gabriele Droege Botanic Garden and Botanical Museum Berlin-Dahlem Freie Universität Berlin

Collection Management

• Open source software for specimen and/or DNA data management– list of recommendations