The Effects of Resveratrol and The Effects of Resveratrol and Alcohol on Short Term Memory in Alcohol on Short Term Memory in

Drosophila MelanogasterDrosophila Melanogaster

By Paul MascaliBy Paul Mascali

NeedNeed

AlcoholAlcohol

A drug found A drug found through the through the fermentation of fermentation of fruitsfruits

Alcohol deactivates Alcohol deactivates the genes protein the genes protein kinase and sirtuin 1kinase and sirtuin 1

ResveratrolResveratrol

Resveratrol is a chemical Resveratrol is a chemical that is found in plantsthat is found in plants

Resveratrol activates the Resveratrol activates the protein kinase and sirituin protein kinase and sirituin 11

Memory Perception Memory Perception

Resveratrol on lifespan in Drosophila and C. Resveratrol on lifespan in Drosophila and C. ElegansElegans

Bass, 2007 Bass, 2007 Resveratrol was inserted Resveratrol was inserted

in the drosophila food at in the drosophila food at 1, 3.2, 10, 32, 100, 200, 1, 3.2, 10, 32, 100, 200, and 1000 mMand 1000 mM

Resveratrol was Resveratrol was dissolved at a 1:1000 to dissolved at a 1:1000 to molten agar NGM ratio molten agar NGM ratio during preparation of during preparation of plates for C. Elegansplates for C. Elegans

Drosophila Memory Drosophila Memory Notch is required for long-term memory in Drosophila

Asaf Presente

Molecular Genetic Analysis of Ethanol Molecular Genetic Analysis of Ethanol Intoxication in Intoxication in Drosophila melanogasterDrosophila melanogaster

Ulrike Heberlien

Alcohols Effect on Alcohols Effect on MemoryMemory

Genetic Dissociation of Ethanol Sensitivity and Memory Formation in Genetic Dissociation of Ethanol Sensitivity and Memory Formation in Drosophila Drosophila MelanogasterMelanogaster

Holly LaFerriere 2007 Holly LaFerriere 2007

A Publication of The Genetics Society of America A Publication of The Genetics Society of America

PurposePurpose

The purpose of this experiment is to see The purpose of this experiment is to see how alcohol and resveratrol effects the how alcohol and resveratrol effects the

short term memory in Drosophila short term memory in Drosophila MelanogasterMelanogaster

Hypothesis Hypothesis

Null Hypothesis: There will be no significant Null Hypothesis: There will be no significant difference between the control group, the difference between the control group, the group exposed to alcohol, and the group group exposed to alcohol, and the group exposed to resveratrol.exposed to resveratrol.

Alternative Hypothesis: The groups that are Alternative Hypothesis: The groups that are exposed to resveratrol will show more of an exposed to resveratrol will show more of an increase in short term memory than the control increase in short term memory than the control and the groups exposed to alcohol and the groups exposed to alcohol

Memory Testing Memory Testing

Memory testingMemory learning

Exposure to AlcoholExposure to Alcohol

Testing Chamber

Air Pump

Ethanol Beaker

Exposure to Resveratrol Exposure to Resveratrol

Methodology Methodology Wild Type Drosophila

Control Group

Never exposed to Resveratrol or

ethanol

N=200

Alcohol exposed Group

Exposed to 200 Proof ethanol for 7 minutes

N=200

Resveratrol exposed Group

Exposed to 1000 Micro Liters of Resveratrol

N=200

Resveratrol and Alcohol exposed Group

Exposed to 200 proof ethanol for 7 minutes and 1000 Micro Liters

of Resveratrol

N=200

Flies put through shock training test

Flies tested for memory Data put into SPSS for a one-way ANOVA and scheffe post-hoc test

Budget Budget

Vendor Cat.# Page Describe Item Quantity Unit Price Total Price

Carolina Np-86-1283 811 95% Ethanol 3 23.5 70.5

N/A     Air pump 1 Already obtained  

N/A    

Drosophila Exposure Chamber 1 Already obtained  

N/A     Ethanol beaker 1 Already obtained  

N/A     Short term memory

monitor  1   Already obtained  

N/A 4-

methycyclihexanol 1 Already obtained

Sigma-Aldrich Online Catalog

R5010-500MG Resveratrol 1 $281.50 $281.50

Do-abilityDo-ability

Exposure to Resveratrol Exposure to Alcohol

Bibliography Bibliography

Bibliography Bibliography Tabakoff,B; Conrell, N; &Hoffman, P.L. Alcohol tolerance. Annals of Emergency Medicine 15(9):10005-1012, 1986Tabakoff,B; Conrell, N; &Hoffman, P.L. Alcohol tolerance. Annals of Emergency Medicine 15(9):10005-1012, 1986 Kathryn Pillips, Ethanol Tolerance in Temperate Drosophila, Journal of Experimental Biology 209, September 19, 2006Kathryn Pillips, Ethanol Tolerance in Temperate Drosophila, Journal of Experimental Biology 209, September 19, 2006 Heberlein Ulrike. Molecular Genetic Analysis of Ethanol Intoxication in Drosophila Melanogaster. Aug. 2004Heberlein Ulrike. Molecular Genetic Analysis of Ethanol Intoxication in Drosophila Melanogaster. Aug. 2004 Natural selection protected some east Asian Population form Alcoholism Science Daily 2003Natural selection protected some east Asian Population form Alcoholism Science Daily 2003 Jama and Archives journals (2007) Almost one-third of Adults report having some Form of Alcohol use problem during their lifetime. Jama and Archives journals (2007) Almost one-third of Adults report having some Form of Alcohol use problem during their lifetime.

Science DailyScience Daily Sullivan Ashburner Hawley, Drosophila Protocols, Cold Spring Harbor Laboratory Press, 1996Sullivan Ashburner Hawley, Drosophila Protocols, Cold Spring Harbor Laboratory Press, 1996 Mumenthaler, Martin Gender Differences in Moderate drinking effect alcohol Health & Research World Mumenthaler, Martin Gender Differences in Moderate drinking effect alcohol Health & Research World Chein Samson Homophila: human disease gen cognates in Drosophila Nucleic Acids Research Chein Samson Homophila: human disease gen cognates in Drosophila Nucleic Acids Research Steven Gans Fruitflies Used to Examine Alcohol Tolerance 2000Steven Gans Fruitflies Used to Examine Alcohol Tolerance 2000 Humenthaler Martin Gender Differences in Moderate Drinking Effects Alcohol Health & Research WorldHumenthaler Martin Gender Differences in Moderate Drinking Effects Alcohol Health & Research World Windberg Jan Olof Drosophila Melanogaster Alcohol Dehydrogenase: Pridyct- Inhibition 1994Windberg Jan Olof Drosophila Melanogaster Alcohol Dehydrogenase: Pridyct- Inhibition 1994 Ulrike Heverlein Molecular Genetic Analysis of Ethanol Intoxication in Drosohoila Melanogaster 2004 Department of Anatomy and Ulrike Heverlein Molecular Genetic Analysis of Ethanol Intoxication in Drosohoila Melanogaster 2004 Department of Anatomy and

Program in NeuroscienceProgram in Neuroscience Morozava Transcriptional Response to Alcohol exposure in Drosophila Melanogaster; Genome BiologyMorozava Transcriptional Response to Alcohol exposure in Drosophila Melanogaster; Genome Biology White, Raskin H., and Kristina Jackson. "Social and Psychological Influences on Emerging Adult Drinking Behavior." 10 Jan. 2004.White, Raskin H., and Kristina Jackson. "Social and Psychological Influences on Emerging Adult Drinking Behavior." 10 Jan. 2004. Winberg, Jan-Olof, and John McKinley-McKee. "Drosophila Melanogaster Alcohol Dehydrogenase: Product-Inhibition." 1994.Winberg, Jan-Olof, and John McKinley-McKee. "Drosophila Melanogaster Alcohol Dehydrogenase: Product-Inhibition." 1994. Jordi Benach, Silvia, Atrian, Joan Fibla, Roser Gonzaler-Duarte ,and Rudolf Ladenstein, Jordi Benach, Silvia, Atrian, Joan Fibla, Roser Gonzaler-Duarte ,and Rudolf Ladenstein, Structure-fuction relationships in Drosophila Structure-fuction relationships in Drosophila

Melanogaster alcohol Dehydrogenase allozymes, Melanogaster alcohol Dehydrogenase allozymes, Copyright 2000, Center for Structural Biochemistry.Copyright 2000, Center for Structural Biochemistry. Ulrike Heberlein, Fred W. Wolf, Adrian Rothenfluh and Douglas J. Guarnier, Ulrike Heberlein, Fred W. Wolf, Adrian Rothenfluh and Douglas J. Guarnier, Molecular Genetic Analysis of Ethanol Intoxication In Molecular Genetic Analysis of Ethanol Intoxication In

Drosophila Melangaster,Drosophila Melangaster, Copyright 2004, Department of Anatomy and Program in Neuroscience. Copyright 2004, Department of Anatomy and Program in Neuroscience. Medina, J. (1996). Medina, J. (1996). The Clock of Ages. The Clock of Ages. Cambridge: Cambridge University Press.Cambridge: Cambridge University Press. Hyung-Lyun Kang , Seymour Benzer , Kyung-Tai Min, Life extension in Drosophila by feeding a drug. . 2002 JanHyung-Lyun Kang , Seymour Benzer , Kyung-Tai Min, Life extension in Drosophila by feeding a drug. . 2002 Jan