the exchange of genetic material between bacteria or how bacteria acquire resistance to...
TRANSCRIPT
The exchange of Genetic Material between bacteria
or
How bacteria acquire resistance to antimicrobial agents in nature
Modes of genetic exchange
transformation
phage mediated transduction
conjugation
transposition
Transformation of bacteria with linear DNA—some cells are naturally
competent for the uptake of DNA
Plasmids are autonomously
replicating circular DNA
molecules that can be used to artificially
transform bacteria—naturally competent cells can take up a
plasmid, such plasmid does not need to
integrate with the chromosome for maintenance!!
Generalized transduction mediated by bacteriophages
Conjugation—a
mechanism by which
mobilizable plasmids
can be transferred
from one bacterium to another
Note tra genes, oriT and antibiotic resisitance genes
Note conjugation bridge formed between donor and recipient cells
Conjugation—a mechanism by which mobilizable plasmids can be transferred
from one bacterium to another
Mobilizable plasmids can integrate into the bacterial chromosome—therefore
bacterial genes can be transferred.
Transposons that carry antibiotic resistance genes can randomly “hop”
into a bacterial chromosome
Mechanism for transposition
ABR
ABR