The Microscope and Its Historical Development

Plant Cell

Blood

Animal Cell

Historical Development of the MICROSCOPE

Reading Stone

SalvinoD’Armate

1st wearable eyeglasses

Multiple Lens Tube

ZacchariasJanssen

Hans Janssen

Robert Hooke

Microscope Lens

Observed

Pores /

Cells

Anton van Leeuwenhoek

Simple Microscope

Blood, yeast, insects

What is MAGNIFICATION?

What is RESOLUTION?

RESOLUTION

SPECIMENobjects being studied under the

microscope

PARTS AND FUNCTIONS OF A

COMPOUND LIGHT MICROSCOPE (CLM)

PuzzleEECIPEEY

PuzzleNEFITUATEDDNSMJ KNOB

PuzzleMAR

PuzzleBESA

PuzzleTVBEIJECO

PuzzleVOGVELNIR PESNIECEO

PuzzleTSGAE

PuzzleROIMMR

EYE PIECEIt is used to see the

objects under study.]Usually 10 X or 15 X

magnification.

DRAW TUBE

Reflects light up to the viewers eye.

It connects the eyepiece to the objective lenses

REVOLVING NOSE PIECE/TURRET

It allows the rotation of the lenses while

viewing.

OBJECTIVES

LPO- 10X magnification

HPO- 40X magnification

OIL IMMERSION- 100X magnification

SCANNING-4X magnification

STAGE

The platform that is used for placing the

slides under observation

STAGE CLIPS

Used to keep the slide in place

DIAPHRAGM

Helps in controlling the amount of light

that is passing through the opening of the

stage.

LIGHT SOURCE/ ILLUMINATOR

A steady light source (110 volts) used in place of a mirror.

If the microscope has a mirror, it is used to reflect light from an

external light source up through the bottom of the stage.

ARMSupports the tube of the microscope and connects to the base

of the microscope

COARSE ADJUSTMENT KNOB/COARSE FOCUS

Used for focus on scanning. Usually the

low power lens is used enabling the

movement of the tube.

FINE ADJUSTMENT

Moves the body tube for focusing

the high power lens.

COARSE ADJUSTMENT KNOB and FINE ADJUSTMENT KNOB

BASEProvides basal support

for the microscope

SUMMARY

SUMMARY

EXERCISE: Label the parts of the microscope

Proper Procedures

1. Carry the microscope with one hand under the base while grasping the arm with the other hand

2. Place microscope ~5 inches from the edge of the table

Be careful of the cord when moving around the lab tables!!

Proper Procedures

3. Rotate the nosepiece to obtain the proper objective lens

Make sure the lens clicks into place!!

4. Look through the eyepiece and switch on the lamp

This is your field of view!

Proper Procedures

5. Place slide on stage, center specimen over stage

6. Locate specimen on low power using coarse adjustment

7. Carefully switch to medium or high power

8. Focus image with fine adjustment

Avoid damaging slide!Do not use course adjustment with high power!

Storing the Microscope

1. the 10X objective is in place

2. the stage is all the way down

3. the power is off

4. the cord is wrapped around the base

Four steps prepare the microscope for storage: