the most powerful hyperspectral imaging software available
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Software forHyperspectral Imaging
Software forHyperspectral Imaging
Software forHyperspectral Imaging
Import Filters
Agilent Resolutions ProBruker OPUSGrams-3DMATLABPerkinElmer SpectrumSpotlightThermo-Nicolet AtlµsRenishaw WiRE 4.0WITec (ASCII), Kaiser-Holospec,Horiba Jobin-Yvon (ASCII)
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Chemical imagingHierarchical cluster imagingFrequency/ imagingPCA, n-findr & VCA imagingFuzzy C-means cluster imagingk-means cluster imagingANN imaging (SNNS)Imaging interface to theNeuroDeveloper (artificial neuralnetwork analysis)
FWHM
Savitzky-Golay derivativesSmoothingNormalizationAbsorbance/transmission conversionSpectral preprocessing: cut &interpolationPCA based noise reductionNon-linear baseline correctionWater vapor correctionSpectral quality testsCosmic ray removalSpatial preprocessing: crop & binningSpatial filtering, 3D-FSD
Preprocessing
imaging methods
download a demo version athttp://www.cytospec.com
for more informationInquire info at
CytoSpecc/o Dr. P. Lasch
Parkstraße 29Berlin D-13086GERMANY
Fax: +49-321 21026149
CytoSpec Inc. http://www.cytospec.com CytoSpec Inc. http://www.cytospec.com CytoSpec Inc. http://www.cytospec.com
The Most PowerfulHyperspectral
ImagingSoftware Available
Rel. 2.0
absorbancehighlow
500 µm
Info at Info@CytoSpec .com internet: http://www.CytoSpec .comCytoSpec - software for hyperspectral imaging
Infrared class mean spectra as obtained by the 'calculateaverage spectra' option of CytoSpec's Hierarchical clusterimaging routine (6-class classification approach, see panel a).class mean spectra were obtained from original absorbancespectra (left) and first derivative spectra (Right). Spectra arecolor encoded in accordance to the color scheme used inPanel A.
FE
C
A B
D
1050115012501350
second derivatives
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1202
1282
1336
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21
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44
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79
1402
2
3
wavenumbers (cm )-1
1100130015001700
wavenumbers (cm )-1
absorbance
(AU
)
1- colonocytes2 - central parts of the crypts
(goblet cells, lumen)3 - lamina propria mucosae
1
2
3
EXAMPLE: IR imaging of a tissue specimen from thehuman colon mucosa. Panels A & B: IR images obtained byagglomerative hierarchical clustering (6 or 15 cluster).Panels C & D: Image reassembling based on k-means clustering(8 or 15 cluster). Panel E: Microphotograph of the tissuearea examined (1.34 x 1.39 mm2or 232 x 223 spectra).Panel F: Images as obtained by chemical imaging (area of thebaseline corrected amide I band 1620-1680 cm ).Preprocessing and image reassembling was carried out byusing the CytoSpec software package.
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What Is CytoSpec?
The multivariate imaging software packageCytoSpec represents a novel approach forcreating pseudo-color displays of spectral imagingdata via statistical methods that utilize the entirespectral information. It is laid out and optimizedto work on 3-dimensional spectral data sets fromimaging or mapping infrared or raman experiments.Native data formats from from various instrumentmanufacturers can be analyzed.
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What is new in CytoSpec 2.0?
CytoSpec̀ s graphical user interface has beenredesigned and now features a clean look thatmakes it simpler and quicker to use
New submenus for spectral preprocessing and spatialpreprocessing
New submenu 'image processing' that allows to performclustering of chemical images
New or renamed functions for spatial preprocessing:crop, binning, spatial filtering, 3D-Fourierself-deconvolution (FSD)
n-findr multivariate imaging function - an endmemberextraction method
New function FWHM maps (submenu univariate imaging)
The import filter for binary Thermo/Atlus data fileshas been completely revised. This filter supports nowimporting of Atlus mapping version 7 data files
Import filter for Renishaw *.wdf spectral maps. Withversion 2.00.02 CytoSpec supports importing of WiRE4.0 *.wdf mapping data files.
Colormaps i -jet, i -hsv and i -hot allow displayingcolormaps which are useful to produce
from spectral second derivatives
Memory option compress has been deactivated - thisfunctionality is obsolete with the 64-bit version ofthe CytoSpec software
With CytoSpec 2.0 the function chemical movie hasbeen added to the demo and commercial programversions
The figure display options has been removed - thisfunctionality is now available from the redesignedgraphical user interface of CytoSpec 2.0
Many code improvements & bug fixes
A trial version of CytoSpec canbe downloaded from the CytoSpecwebsite: http://www.cytospec.com
invertedchemical images
Software forHyperspectral Imaging