the most versatile automated patch clamp system just got ... sheet... · fluxion is excited to...
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IonFlux F1 Giga-Ohm Seal Plates The most versatile automated patch clamp system just got better
PRODUCT DATA SHEET
Fluxion is excited to introduce single-cell recording plates for its IonFlux 16 and IonFlux HT systems. While Fluxion’s ensemble recording plates set the standard for consistency and throughput, single-cell giga-ohm seal plates are ideal for cells exhibiting heterogene-ous expression profiles and for detailed kinetic studies. The new single-cell recording IonFlux F1 plates are fully compatible with existing IonFlux systems. F1 plates are handled exactly like Fluxion’s ensemble-recording plates, and utilize similar recording protocols. Software-selectable amplifier gain settings are used to toggle between the two recording modes, so switching modes couldn’t be easier.
All the benefits you expect from the IonFlux Automated Patch Clamp System, now with single-cell giga-ohm seal plates
Single cell plate design
Ensemble plate design
Ensemble or single-cell giga-ohm seal Voltage and ligand-gated ion channels Continuous perfusion and rapid compound application Affordable — IonFlux 16 similarly priced to a manual rig Easy-to-use plate reader operation Small bench-top footprint High throughput — up to 10,000 data points per day (per reader)
•• • • • • • • Integrated temperature control
Comparison chart - F1 Single-Ce ll Plate vs. Ensemble Plate With the introduction of the F1single-cell giga-ohm patch clamp plate, the IonFlux system covers a greater range of applications than ever before. This chart lists the relative performance of the two plate types and typical applications. Both plates are fully compatible with the same IonFlux systems; no hardware change is required.
Current distribution for ASIC ensemble recording
IonFlux F1 Giga-ohm Seal Plates The most versatile automated patch clamp system just got better
Current distribution for single cell recording (ASIC, pH5.5)
Ensemble Plate F1 Single-cell plate...
elbmesnE etubirttA plate F1 Single-cell plate
Cells measured per recording 20 cells in parallel 1 cell
Typical seal resistance 200 MOhm >1 G-Ohm
Number of recordings per plate 16 to 64 recordings (IF HT) 16 to 64 recordings (IF HT)
Compound application timescale 100 ms 25 ms
Typical applications Compound profiling, screening Mutant characterization, kinetic analysis, heterogeneous cell lines
Nav 1.8 ensemble currents at V=0mV (raw data, no comp.) Nav 1.8 single cell currents at V=0mV (raw data, no comp)
Seal distribution for ensembles (hERG, avg. per cell) Seal distribution for single cells (Nav 1.8, 1/2 plate)
-1000 0 1000 2000 3000 4000 5000 6000 7000 80000
2
4
6
8
10
12
Cou
nt (n
umbe
r of c
ells
)
Seal (Mohm )
-5000 -4000 -3000 -2000 -1000 0 10000
2
4
6
8
10
12
Cou
nt (n
umbe
r of c
ells
)
Current (pA)-50,000 -40,000 -30,000 -20,000 -10,000 0 10,0000
2
4
6
8
10
Cou
nt (n
umbe
r of e
nsem
bles
)
C urrent (pA )
-1000 -500 0 500 1000 1500 2000 2500 30000
5
10
15
20
Cou
nt (n
umbe
r of e
nsem
bles
)
Seal (Mohm )
© 2011 Fluxion Biosciences, Inc. All rights reserved. “IonFlux” is a trademark of Fluxion Biosciences, Inc. 1081-01 11/03/11
PRODUCT DATA SHEET