the obtaining of a new natural vegetal product hof.lipomin having hypolipemiant properties gabriela...
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THE OBTAINING OF A NEW NATURAL VEGETAL PRODUCT HOF.LIPOMIN HAVING HYPOLIPEMIANT PROPERTIES
GABRIELA DENISA RIZEA1*, DENISA MIHELE2, DANIELA IONESCU1, ŞTEFAN MANEA1, MARIA IVOPOL1
OBJECTIVES The obtaining of the new natural vegetal hypolipemiant product named Hof.Lipomin having in composition the inulinic concentrated and degreased extract of Helianthi tuberosus tuber, the Linseed oil, the extracts of Allii sativi bulbus and Allium porrum.The determination of the chemical composition of the obtained product, of its physical chemical and microbiological properties as well as the emphasing of its hypolipemiant action on the laboratory animals and in clinical studies.MATERIALS AND METHODS The chemical compostion was established using HPLC, UV-VIS, and AEA Spectrometry methods. The hypolipemiant activity was determined on the white masculs Wistar rats, having 220-250g. The clinical studies were realized on 30 patients having dislipemia monitorized over a period of 120 days.RESULTS Biochemical parameters of the animals treated with Hof.Lipomin, having WR 1339 Triton induced hyperlipemia
Figure 1. The effect of Hof.Lipomin and its constituients Figure 2. The effect of Hof.Lipomin on LDL- cholesterol of the rats on total serum lipids of the rats
CLINICAL STUDIES
Nr.crt.
Patients
The before cholesterol
total [mg/dL]
The total cholesterol
after[mg/dL]
Triglycerides before
[mg/dL]
Triglycerides after
[mg/dL]
1 V.A. 240 220 100 201
2 S.F. 260 208 80 60
3 R.L. 180 167 101 102
4 S.A. 218 220 102 60
5 A.V. 157 180 60 210
6 S.P. 236 202 180 190
7 P.I. 300 240 430 180
8 G.M. 282 262 110 80
9 A.V. 180 182 140 90
10 D.G. 260 180 130 132
11 S.C. 180 170 150 160
12 I.V. 310 230 350 170
13 P.M. 220 180 170 240
14 S.I. 260 250 330 430Average 234,5 ±
20,15206,5 ±
15,5173,48± 20,15 178,81±
15,15
Figure 3. Graphic representation the obtained results for the total cholesterol
Figure 4. Graphic representation the obtained results for the serum triglycerides
CONCLUSIONS: The new product is in accordingly to the FR X concerning the phisico-chemical and microbiological properties. Hof.Lipomin doesn’t present cytotoxicity on the cells cultures. The studies realized on the animals experiments emphasized the lowering of the total serum lipids, of the total serum cholesterol and of serum triglycerides. The clinical studies concerning the application of the Hof.Lipomin in treatment of dislipemies point aut the decrease of total serum lipids, serum cholesterol, LDL cholesterol and serum triglycerides. The ratio total cholesterol /HDL cholesterol was improved by the total serum cholesterol lowering and by the HDL increasing. The obtained results prove that it is possible to decrease the risk of dangerous cardio-vascular events using Hof.Lipomin.
REFERENCES1. Arnault, J, Auger J., Selenocompousse in garlic and onion, Journal of Chromatography, 2006,112 (1-2), 23-30.2. Mihele D., Biochimie chimica, Metode de laborator , ed. III, Ed. Medicala, 2007, Bucuresti3.Rizea G.D., Popescu M., Ionescu D., Mihele D., Manea Ş., 2012 ,,Comparative Determinations of Antioxidant Free Radical Scavenging Polyphenols, in Certain Natural Products by HPLC Methods and UV-Vis Spectrophometry’’, Rev.Chim.(Bucharest),63(11), 1085-1088.