The Polymerase Chain Reaction

Some milestones

In molecular biology

recognised by the

award of the Nobel

prize

Table (continued)

Number of publications citing the polymerase chain reaction from

1985 to 1999

Klenow fragment

used for DNA

sequencing

Primers for DNA polymerase

Primer design

The use of PCR to amplify DNA moleculesin vitro

The basic polymerase chain reaction

The polymerase

chain reaction

PCR amplification

of DNA Fragment

The PCR cycle

The PCR leads

to amplification

of the target

sequences

Mismatching primers

can lead to the

efficient amplification

of undesired sequences

Cloning DNA using

the PCR and the

consequences of

polymerase mistakes

Amplifying cloned DNA from vectors

Alu-PCR is used to isolate human-specific DNA sequences

Asymmetric PCR

For producing single-

stranded DNA

for sequencing

Detecting cancer cells using the PCR

Determining sex

of fetuses at risk

for X-linked

inherited disorders

Nested Primers increase the specificity of a PCR

Nested PCR

Gene linkage

analysis using

single sperm

Reverse transcriptase PCR (RT-PCR)

Competitor

RT-PCR

Inverse PCR

Some variants of the basic PCR Process

Visualisation of PCR products