the proteomeworks system is the global alliance of micromass and bio-rad laboratories dedicated to...
TRANSCRIPT
The ProteomeWorks System is the global alliance of Micromass and Bio-Rad Laboratories dedicated to furthering proteomics research.
The ProteomeWorks System~ New Mass Spectrometry Systems ~
At Pittcon 2001 Micromass introduced a range of e-MSTM enabled MALDI-TOF MS / MS-MS & ESI LC-MS-MS systems and Mass-InformaticsTM Software to leverage The ProteomeWorksTM System
• M@LDI HT TM
• Q-Tof UltimaTM
• Q-Tof microTM
Micromass’ new networked MALDI TOF-MS & sample management system
“2D-gel-MS analyser for proteomics factories”
M@LDI HT TM [new instrument]
M@LDI HT TM - Networked MALDI-TOF MS
REFLECTRON Peptide Mass Fingerprinting
LINEAR Intact Proteins
ELECTRO MAGNETIC GRIPPER
TARGET PLATE CASSETTE 2
X - Y ROBOTIC ARM
TARGET PLATE CASSETTE 1
HORIZONTAL AXIS MOTOR
VERTICAL AXIS MOTOR
BAR CODE SCANNERVACUUM LOCK LID
IN COLUMNOUT COLUMN
VERTICAL FLIGHT TUBE AND REFLECTRON HOUSING
M@LDI HT TM - Sample Management System 80 x 96/384-Sample Targets (bar coded)
M@LDI HT TM - Networked MALDI-TOF MS
• Linear and reflectron ion optics options• 80 x 96/384-Sample target management system• Bar coded sample target plates• Automated Laser Positioning System (ALPS) for
sample target alignment and sample indexing• Intelligent acquisition / calibration algorithm• Web-enabled Client-Server Mass-Informatics for
interrogation of non-redundant protein dB
…converts samples into knowledge not ‘just’ spectra
ProteinLynxTM Global SERVER
web-enabled Mass-informatics
ProteinLynxTM Global SERVER export to PDQuest Annotation Tools
PDQuest Annotation Tools
C L I C K
MS & MS-MS Techniques for Peptide / Protein Analysis
Technique Data Type Dynamic
Range
Specificity Speed Data InterpretationConstraints
MS MALDI MH + Low Low High Known Protein dB
MALDI PSD MH + Low Low Medium Known Protein dB
MS-MSMALDI MH +
Fragments
Low Medium High Known Protein dB
Known nucleotide dB
ESI + HPLC MH 2 ++ &
Fragments
High High Medium Known Protein dB
Known nucleotide dB
*ESI + HPLC(de novo)
Amino Acid
Sequence
High High Medium Homologues (BLAST)
Time (PCR)
Q-Tof UltimaTM
family
[new instruments]
DDATM
Micromass’ SuperPremium family of MALDI MS-MS and ESI MS-MS systems for PROTEOMICS and structural analysis
DDATM
Q-Tof Ultima…the SuperPremium family of MS-MS
• Q-Tof Ultima MALDI• The high throughput MALDI MS-MS
• Q-Tof Ultima API• The SuperPremium API LC-MS-MS
• Q-Tof Ultima GLOBAL• The flexible research platform for API LC-MS-MS / MALDI MS-MS
•
Q-Tof UltimaTM MALDI [new instrument]
• Dedicated MALDI MS-MS system• V & W-OPTICS options • ProteinLynx Global SERVER• e-MS enabled
Q-Tof Ultima MALDI with VOPTICS
10,000 FWHM
Q-Tof Ultima MALDI with WOPTICS
20,000 FWHM
MALDI MS-MS is superior to PSD
ADH 1 pm/µl PSD
ADH 1 pm/µl Q-Tof Ultima MALDI MS-MS
ProteinLynxTM Global SERVER ...Matching MS-MS spectra to dB of known protein or nucleotide sequence.
Q-Tof UltimaTM API [new instrument]• Dedicated API (ESI) LC-MS-MS system• V & W-OPTICS options • DDA - Precursor Ion Discovery (patent applied for)
– “Neutral Loss”– “Precursor Ion”– High resolution– Exact mass– Compatible with HPLC
• ProteinLynx Global SERVER• e-MS enabled
Q-Tof Ultima API with WOPTICS
20,000 FWHM
Q-Tof UltimaTM API ...comparison of V & W-OPTICS Resolution
The superior peptide sequence coverage provided by ESI MS-MS data enables de novo sequencing
Sequencing results from MS-MS of m/z 841.5
Q-Tof UltimaTM GLOBAL [new instrument]• Flexible API & MALDI MS-MS research platform• API / MALDI changeover without venting• V & W-OPTICS options • LC-DDA-MS
– Precursor ion discovery– “Neutral Loss”– “Precursor Ion”– Exact mass
• ProteinLynx Global SERVER• e-MS enabled
Q-Tof Ultima GLOBAL
Q-Tof microTM
[new instrument]
Micromass’ finest API Q-Tof technology for
structural analysis & protein characterisation…
• SMALLER• SMARTER• WIRED
DDATM
Q-Tof microTM
…SMALLER• ZSPRAYTM nanoflow ESI inlet technology• 5000 FWHM resolution• 5ppm RMS exact mass measurement• Dynamic External Calibration (DXCTM)• 20000 m/z range
Q-Tof microTM
…SMARTER …WIRED• Data Directed Analysis (DDA)
– Automates analysis of protein digests by LC-DDA-MS• Precursor Ion Discovery (patent applied for)
– “Neutral Loss”– “Precursor Ion”– High resolution– Exact mass
• Compatible with HPLC• Maximising data quality
Data Directed Analysis DDATM
Exclusion of 1+
background ions
The New e-MSTM Enabled Mass Analysers of The ProteomeWorksTM System...
msinnovation.com
The ProteomeWorks System is the global alliance of Micromass and Bio-Rad Laboratories dedicated to furthering proteomics research.