the rapid microbiology company b a c t i f l o w real-time microbiology analysis in food, beverages...
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The Rapid Microbiology Company
B a c t i B a c t i F l o wF l o w
Real-time microbiology analysis in Food, beverages & cosmetics
Compact Microbial AnalyserCompact Microbial Analyser
The Rapid Microbiology Company
Based on Proven TechnologiesBased on Proven Technologies
Fluorescent cell labelling (Fluorassure®) Same reagent technologies as used on D-Count® & ChemScan® RDI Validated on more than 500 strains
Bench Top Microbial Analyser Compact Flow cytometer specifically designed for microbial detection Laser based detection Simple to operate
Data acquisition, analysis and real time result display Automatic data interpretation Quantitative results in minutes
The Rapid Microbiology Company
FluorassureFluorassure Viability Markers Viability Markers
Criteria of Viability :
Enzyme activity Membrane integrity
Enzyme
Viability substrate
Free fluorochromeBacteria
Applicable for :
Total Viable Count Yeast & Moulds Specifics (on going)
The Rapid Microbiology Company
Quantitative Microbial CountingQuantitative Microbial Counting
Detector 1
Detector 2
Labelled Sample
Laser
Sample
Flow Cell :
Linear fluid stream
Cells Separated into single entities
Sensitive detectors
B a c t i B a c t i F l o wF l o w
The Rapid Microbiology Company
Pure Culture CorrelationPure Culture Correlation
R2 = 0,9919
1
10
100
1000
10000
100000
1000000
1 10 100 1000 10000 100000 1000000Plate Count
Bac
tiF
low
( M
icro
-org
anis
ms
/ m
l)
Combined plot of all micro-organisms analysed
Staphylococcus aureusEscherichia coliBacillus circulansBacillus licheniformisEnterobacter cloacaeCandida albicansPenicilium decumbeusAspergillus niger
The Rapid Microbiology Company
R a p i dR a p i d
Time to result:
10 minutes labelling prior to analysis
1 minute analysis per sample
Simple sample preparation adapted to product
matrices
Labelling 10 min. Counting 1 min.
No cell multiplication required
The Rapid Microbiology Company
S e n s i t i v eS e n s i t i v e
Combining Speed with Sensitivity
Quantitative counting cell by cell
Sensitivity of detection less than 100 cells / gram.
Linear response up to 100,000 micro-organisms
Direct Counting in less than 15 minutes
Protocol available for Presence / Absence within 24 hours
The Rapid Microbiology Company
E a s y t o U s eE a s y t o U s e
Ready to use Reagents
Simple preparation for sample products
Validated protocols for a full range of applications
Fully automated detection and counting
No data interpretation required
Full data traceability
PC with Windows XP interface
The Rapid Microbiology Company
B a c t i B a c t i F l o w F l o w A p p l i c a t i o n sA p p l i c a t i o n s
Fruit based Products Dairy Products Cosmetics
WaterPure cultures