the secretory pathway - science.umd.eduscience.umd.edu/cbmg/faculty/song/688d/lectures/secretory...
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![Page 1: The secretory pathway - science.umd.eduscience.umd.edu/cbmg/faculty/song/688D/Lectures/Secretory pathway.pdf · Clathrin + GGA Clathrin + AP2 complexes ARF ARF trans-Golgi to endosome](https://reader030.vdocument.in/reader030/viewer/2022040708/5e0ac5e0c8bcf600cd14ba82/html5/thumbnails/1.jpg)
The secretory pathway
![Page 2: The secretory pathway - science.umd.eduscience.umd.edu/cbmg/faculty/song/688D/Lectures/Secretory pathway.pdf · Clathrin + GGA Clathrin + AP2 complexes ARF ARF trans-Golgi to endosome](https://reader030.vdocument.in/reader030/viewer/2022040708/5e0ac5e0c8bcf600cd14ba82/html5/thumbnails/2.jpg)
Addition and initial processing of N-linked oligosaccharine in the rough ER of
vertebrate cells
O-linkerSer/Thr
N-linkedAsn-X-SerAsn-X-Thr
![Page 3: The secretory pathway - science.umd.eduscience.umd.edu/cbmg/faculty/song/688D/Lectures/Secretory pathway.pdf · Clathrin + GGA Clathrin + AP2 complexes ARF ARF trans-Golgi to endosome](https://reader030.vdocument.in/reader030/viewer/2022040708/5e0ac5e0c8bcf600cd14ba82/html5/thumbnails/3.jpg)
Processing of N-linked oligosaccharine chains on glycoprteins in Golgi
![Page 4: The secretory pathway - science.umd.eduscience.umd.edu/cbmg/faculty/song/688D/Lectures/Secretory pathway.pdf · Clathrin + GGA Clathrin + AP2 complexes ARF ARF trans-Golgi to endosome](https://reader030.vdocument.in/reader030/viewer/2022040708/5e0ac5e0c8bcf600cd14ba82/html5/thumbnails/4.jpg)
Formation and rearrangement of disulfide bonds by protein disulfide isomerase (PDI)
![Page 5: The secretory pathway - science.umd.eduscience.umd.edu/cbmg/faculty/song/688D/Lectures/Secretory pathway.pdf · Clathrin + GGA Clathrin + AP2 complexes ARF ARF trans-Golgi to endosome](https://reader030.vdocument.in/reader030/viewer/2022040708/5e0ac5e0c8bcf600cd14ba82/html5/thumbnails/5.jpg)
Chaperon proteins facilitate folding and assembly of protein
![Page 6: The secretory pathway - science.umd.eduscience.umd.edu/cbmg/faculty/song/688D/Lectures/Secretory pathway.pdf · Clathrin + GGA Clathrin + AP2 complexes ARF ARF trans-Golgi to endosome](https://reader030.vdocument.in/reader030/viewer/2022040708/5e0ac5e0c8bcf600cd14ba82/html5/thumbnails/6.jpg)
Unfolded protein response
![Page 7: The secretory pathway - science.umd.eduscience.umd.edu/cbmg/faculty/song/688D/Lectures/Secretory pathway.pdf · Clathrin + GGA Clathrin + AP2 complexes ARF ARF trans-Golgi to endosome](https://reader030.vdocument.in/reader030/viewer/2022040708/5e0ac5e0c8bcf600cd14ba82/html5/thumbnails/7.jpg)
Proteasome degradation of misfolded proteins
![Page 8: The secretory pathway - science.umd.eduscience.umd.edu/cbmg/faculty/song/688D/Lectures/Secretory pathway.pdf · Clathrin + GGA Clathrin + AP2 complexes ARF ARF trans-Golgi to endosome](https://reader030.vdocument.in/reader030/viewer/2022040708/5e0ac5e0c8bcf600cd14ba82/html5/thumbnails/8.jpg)
Major types of proteins involved in vesicle budding and fusion with a target membrane
![Page 9: The secretory pathway - science.umd.eduscience.umd.edu/cbmg/faculty/song/688D/Lectures/Secretory pathway.pdf · Clathrin + GGA Clathrin + AP2 complexes ARF ARF trans-Golgi to endosome](https://reader030.vdocument.in/reader030/viewer/2022040708/5e0ac5e0c8bcf600cd14ba82/html5/thumbnails/9.jpg)
Budding of ER vesicles
![Page 10: The secretory pathway - science.umd.eduscience.umd.edu/cbmg/faculty/song/688D/Lectures/Secretory pathway.pdf · Clathrin + GGA Clathrin + AP2 complexes ARF ARF trans-Golgi to endosome](https://reader030.vdocument.in/reader030/viewer/2022040708/5e0ac5e0c8bcf600cd14ba82/html5/thumbnails/10.jpg)
![Page 11: The secretory pathway - science.umd.eduscience.umd.edu/cbmg/faculty/song/688D/Lectures/Secretory pathway.pdf · Clathrin + GGA Clathrin + AP2 complexes ARF ARF trans-Golgi to endosome](https://reader030.vdocument.in/reader030/viewer/2022040708/5e0ac5e0c8bcf600cd14ba82/html5/thumbnails/11.jpg)
Docking and fusion of transport vesicles
![Page 12: The secretory pathway - science.umd.eduscience.umd.edu/cbmg/faculty/song/688D/Lectures/Secretory pathway.pdf · Clathrin + GGA Clathrin + AP2 complexes ARF ARF trans-Golgi to endosome](https://reader030.vdocument.in/reader030/viewer/2022040708/5e0ac5e0c8bcf600cd14ba82/html5/thumbnails/12.jpg)
Vesicle-mediated protein trafficking between the ER and cis-Golgi
Forward (anterograde) transport
Reverse (retrograde) transport
![Page 13: The secretory pathway - science.umd.eduscience.umd.edu/cbmg/faculty/song/688D/Lectures/Secretory pathway.pdf · Clathrin + GGA Clathrin + AP2 complexes ARF ARF trans-Golgi to endosome](https://reader030.vdocument.in/reader030/viewer/2022040708/5e0ac5e0c8bcf600cd14ba82/html5/thumbnails/13.jpg)
![Page 14: The secretory pathway - science.umd.eduscience.umd.edu/cbmg/faculty/song/688D/Lectures/Secretory pathway.pdf · Clathrin + GGA Clathrin + AP2 complexes ARF ARF trans-Golgi to endosome](https://reader030.vdocument.in/reader030/viewer/2022040708/5e0ac5e0c8bcf600cd14ba82/html5/thumbnails/14.jpg)
Fluorescence microscopy of cells producing a GFP-tagged membrane protein
VSVG-GFP temperature sensitive mutants
![Page 15: The secretory pathway - science.umd.eduscience.umd.edu/cbmg/faculty/song/688D/Lectures/Secretory pathway.pdf · Clathrin + GGA Clathrin + AP2 complexes ARF ARF trans-Golgi to endosome](https://reader030.vdocument.in/reader030/viewer/2022040708/5e0ac5e0c8bcf600cd14ba82/html5/thumbnails/15.jpg)
Radioactive amino acids pulse/chase and protein sensitivity to glycosidases
![Page 16: The secretory pathway - science.umd.eduscience.umd.edu/cbmg/faculty/song/688D/Lectures/Secretory pathway.pdf · Clathrin + GGA Clathrin + AP2 complexes ARF ARF trans-Golgi to endosome](https://reader030.vdocument.in/reader030/viewer/2022040708/5e0ac5e0c8bcf600cd14ba82/html5/thumbnails/16.jpg)
Genetic approach - Yeast sec mutants
![Page 17: The secretory pathway - science.umd.eduscience.umd.edu/cbmg/faculty/song/688D/Lectures/Secretory pathway.pdf · Clathrin + GGA Clathrin + AP2 complexes ARF ARF trans-Golgi to endosome](https://reader030.vdocument.in/reader030/viewer/2022040708/5e0ac5e0c8bcf600cd14ba82/html5/thumbnails/17.jpg)
Cell free system