the thinprep ® paptest ™ paul sultana mls thursday, 22 october 2015
TRANSCRIPT
![Page 1: The ThinPrep ® PapTest ™ PAUL SULTANA MLS Thursday, 22 October 2015](https://reader035.vdocument.in/reader035/viewer/2022062309/56649ee65503460f94bf5c49/html5/thumbnails/1.jpg)
The ThinPrep® PapTest™
PAUL SULTANA
MLSSaturday 22 April 2023
![Page 2: The ThinPrep ® PapTest ™ PAUL SULTANA MLS Thursday, 22 October 2015](https://reader035.vdocument.in/reader035/viewer/2022062309/56649ee65503460f94bf5c49/html5/thumbnails/2.jpg)
Background
• The conventional smear has been the most successful screening test
• Screening every 3-5years has resulted in a 70% reduction in incidence
![Page 3: The ThinPrep ® PapTest ™ PAUL SULTANA MLS Thursday, 22 October 2015](https://reader035.vdocument.in/reader035/viewer/2022062309/56649ee65503460f94bf5c49/html5/thumbnails/3.jpg)
Why LBC (Liquid based cytology) has been introduced in the Malta
• Continuing improvement to the Cervical screening Programme
• Limitations of conventional cytology• Modernisation of the technique• Future benefits
– Extra tests – HPV, chlamydia, Neiseria Gonorrhoea.
![Page 4: The ThinPrep ® PapTest ™ PAUL SULTANA MLS Thursday, 22 October 2015](https://reader035.vdocument.in/reader035/viewer/2022062309/56649ee65503460f94bf5c49/html5/thumbnails/4.jpg)
Limitations Of Conventional Smear (From UK studies)
False Negative Rate of up to 55%1
Sampling and interpretive errors
Ambiguous reports of 6.4%2
70% are truly negative
30% represent more severe abnormality
Inadequate specimens of 9.7% 2
1. Hutchinson et al., AJCP, Vol 101-2; 215-219, 2. DOH Statistical Bulletin 2000/2001
![Page 5: The ThinPrep ® PapTest ™ PAUL SULTANA MLS Thursday, 22 October 2015](https://reader035.vdocument.in/reader035/viewer/2022062309/56649ee65503460f94bf5c49/html5/thumbnails/5.jpg)
Sources Of False Negatives
• Sampling issues (70%)
– cells not collected on the sampling device– cells collected, but not transferred to the slide
• Interpretive issues (30%)
– abnormal cells present on slide but either not seen or misinterpreted
![Page 6: The ThinPrep ® PapTest ™ PAUL SULTANA MLS Thursday, 22 October 2015](https://reader035.vdocument.in/reader035/viewer/2022062309/56649ee65503460f94bf5c49/html5/thumbnails/6.jpg)
How LBC is different
• Easier method for smear takers
• Technology diminishes blood, mucus & polymorphs
• Thin layer preparation
• Smaller area of the slide to screen
![Page 7: The ThinPrep ® PapTest ™ PAUL SULTANA MLS Thursday, 22 October 2015](https://reader035.vdocument.in/reader035/viewer/2022062309/56649ee65503460f94bf5c49/html5/thumbnails/7.jpg)
Conventional smear
![Page 8: The ThinPrep ® PapTest ™ PAUL SULTANA MLS Thursday, 22 October 2015](https://reader035.vdocument.in/reader035/viewer/2022062309/56649ee65503460f94bf5c49/html5/thumbnails/8.jpg)
What we see under the microscope conventional smear
![Page 9: The ThinPrep ® PapTest ™ PAUL SULTANA MLS Thursday, 22 October 2015](https://reader035.vdocument.in/reader035/viewer/2022062309/56649ee65503460f94bf5c49/html5/thumbnails/9.jpg)
The prepared slide with the new ThinPrep
![Page 10: The ThinPrep ® PapTest ™ PAUL SULTANA MLS Thursday, 22 October 2015](https://reader035.vdocument.in/reader035/viewer/2022062309/56649ee65503460f94bf5c49/html5/thumbnails/10.jpg)
What we see under the microscope. Notice the clean back ground and how well the cells are dispersed rendering easier to interpretation.
![Page 11: The ThinPrep ® PapTest ™ PAUL SULTANA MLS Thursday, 22 October 2015](https://reader035.vdocument.in/reader035/viewer/2022062309/56649ee65503460f94bf5c49/html5/thumbnails/11.jpg)
NICE recommendation is based on:
• Systematic review of literature relating to efficacy of LBC undertaken as part of the HTA programme
• New evidence from the 3 pilot sites at Bristol, Norwich and Newcastle
![Page 12: The ThinPrep ® PapTest ™ PAUL SULTANA MLS Thursday, 22 October 2015](https://reader035.vdocument.in/reader035/viewer/2022062309/56649ee65503460f94bf5c49/html5/thumbnails/12.jpg)
![Page 13: The ThinPrep ® PapTest ™ PAUL SULTANA MLS Thursday, 22 October 2015](https://reader035.vdocument.in/reader035/viewer/2022062309/56649ee65503460f94bf5c49/html5/thumbnails/13.jpg)
Summary of Results www.nice.org.uk
“Robust evidence that LBC is a cost effective alternative to conventional Pap for the health service in the UK in terms of life years saved”
Evidence of a reduction in the number of unsatisfactory specimens
Productivity of laboratories increased
Smear takers and laboratory staff liked LBC
Expect LBC will reduce the number of false negatives and the incidence of invasive cancer
![Page 14: The ThinPrep ® PapTest ™ PAUL SULTANA MLS Thursday, 22 October 2015](https://reader035.vdocument.in/reader035/viewer/2022062309/56649ee65503460f94bf5c49/html5/thumbnails/14.jpg)
ThinPrep® Pap Test™Extensively Evaluated
• > 100 published studies • 23 distinct peer-reviewed journals• Health Economic Studies
– AHCPR– Hutchinson– ScHARR
• Three meta-analyses– Bernstein– Abulafia– Klinkhamer
![Page 15: The ThinPrep ® PapTest ™ PAUL SULTANA MLS Thursday, 22 October 2015](https://reader035.vdocument.in/reader035/viewer/2022062309/56649ee65503460f94bf5c49/html5/thumbnails/15.jpg)
Conventional vs. LBC in Scotland
Conventional (%)
LBC
(%)
Unsatisfactory 13.6 1.9
Negative 80.9 91.7
Borderline 2.5 2.6
Mild 2.0 2.3
Moderate 0.6 0.8
Severe 0.4 0.6
Others 0.1 0.1
1 year conventional vs 1 year LBC
![Page 16: The ThinPrep ® PapTest ™ PAUL SULTANA MLS Thursday, 22 October 2015](https://reader035.vdocument.in/reader035/viewer/2022062309/56649ee65503460f94bf5c49/html5/thumbnails/16.jpg)
What is liquid based cytology?
Collection of cellular material into a vial
of preservative fluid
![Page 17: The ThinPrep ® PapTest ™ PAUL SULTANA MLS Thursday, 22 October 2015](https://reader035.vdocument.in/reader035/viewer/2022062309/56649ee65503460f94bf5c49/html5/thumbnails/17.jpg)
ThinPrep® 2000 Processor
![Page 18: The ThinPrep ® PapTest ™ PAUL SULTANA MLS Thursday, 22 October 2015](https://reader035.vdocument.in/reader035/viewer/2022062309/56649ee65503460f94bf5c49/html5/thumbnails/18.jpg)
ThinPrep® 3000 Processor
![Page 19: The ThinPrep ® PapTest ™ PAUL SULTANA MLS Thursday, 22 October 2015](https://reader035.vdocument.in/reader035/viewer/2022062309/56649ee65503460f94bf5c49/html5/thumbnails/19.jpg)
ThinPrep® ProcessIn the laboratory
1. Dispersion 2. Cell Collection 3. Cell Transfer
![Page 20: The ThinPrep ® PapTest ™ PAUL SULTANA MLS Thursday, 22 October 2015](https://reader035.vdocument.in/reader035/viewer/2022062309/56649ee65503460f94bf5c49/html5/thumbnails/20.jpg)
Conventional ThinPrep®
The Difference is Obvious
![Page 21: The ThinPrep ® PapTest ™ PAUL SULTANA MLS Thursday, 22 October 2015](https://reader035.vdocument.in/reader035/viewer/2022062309/56649ee65503460f94bf5c49/html5/thumbnails/21.jpg)
The ThinPrep® PapTest™
PAUL SULTANA
MLSSaturday 22 April 2023