the v.p. c.d.r. (vibrating probe)
DESCRIPTION
The V.P. C.D.R. (Vibrating Probe). Presented by Zac Eichmeyer And BEMsS (BioElectromagnetic student Society). What's a Vibrating Probe (V.P.)?. The V.P. is an instrument capable of measuring current densities in a biological medium Capable of measuring 10 nA/cm 2 - PowerPoint PPT PresentationTRANSCRIPT
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Presented by Zac EichmeyerAnd BEMsS (BioElectromagnetic student Society)
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The V.P. is an instrument capable of measuring current densities in a biological mediumCapable of measuring 10 nA/cm2
It was developed in the 70’s to measure current pulses from an Oocyte
Non-Destructive measurementsCan measure activities in live cells
Offers an order of magnitude increase from current detection methods, and is more versatile than dyes
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How does the V.P. Work? The V.P. works by vibrating a high
impedance probe horizontally ~10m The vibration is controlled by a
Piezoelectric Actuator The Probe is connected to a
Preamplifier which conditions the signal for the Lock-In Amplifier
The Lock-in amplifier locks the probes measurement to that of its vibration so to only read current density at its max displacement Reducing Noise, and giving a
voltage reading at the max of its amplitude
The data is then collected via a data logger and fed into a computer for analyzing
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The V.P. Requirements:The probe
High impedence, greater than 5 MLow length to mass ratio
Piezoelectric Actuator (the bender)Operate between ± 45VBend at least 30m
The Lock-in AmplifierLock on to a 150 Hz – 1kHz signal
The Pre-AmpLow Noise
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The DAQCapable of sampling faster than the vibration
frequency<1 kHz max
The OscillatorCapable of driving a Piezoelectric Actuator at a
frequency less then 1kHzThe Mechanical Manipulator
Connects the probe to the microscopeRigidHave 1 degree of motion (up and down) Thermally and electrically neutral
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To be able to measure current densities <100 nA/cm2
To eliminate/control extraneous noiseTo build the appropriate test setup to
implement the probe with a microscope Implement into current BEMSS researchMeasure current densities in other biological
samplesTrack Ion concentrations as they relate to
Neutrophils
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•The V.P. Overview(subsystems)
•Probes•Piezoelectric Actuator (Bender)•Pre Amp•Lock In Amplifier•Oscillator•DAQ•Housing •Mechanical Manipulator
•Project Overview (Top Down)
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