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International meeting

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August 16, 2011 Faculty Hall,Indian Institute of Science (IISc)

Theoretical andExperimental Immunology

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IISc Mathematics Initiativeand

International Network in Theoretical Immunologypresent an

International Meeting

Theoretical andExperimental Immunology

Faculty Hall,Indian Institute of Science (IISc)

August 16, 2011

GENERAL INSTRUCTIONS

1. Students who have registered “on line” are requested to arrive on August 16,2011 at 8: 30 am in the Faculty Hall, IISc to collect their registration materials.

2. Do note that outstation students will need to make their own arrangementsfor stay. The website does list reasonable places to stay in Bangalore.

3. Traffic in Bangalore is chaotic, so please give yourself sufficient time toensure that you are not late for the meeting.

4. Please display your badge all the time. Entry into the auditoriums, food areasetc. will NOT be permitted without the badge.

5. Kindly switch off your mobile inside the auditorium.

6. Speakers are requested to load their slides prior to the session. Studentvolunteers will help you in the process. Kindly preview your slides before thepresentation.

7. The meeting schedule is rather tight and we kindly request all speakers andchairpersons to please keep to time.

8. For any information, clarifications and assistance, please contact localorganization committee members and student volunteers at the registrationdesk.

iii

CONTENTS

General Instructions iii

MAP of IISc iv

Contents v

Meeting Organization vi

Message vii

Program ix

Abstracts 1

List of Speakers 18

List of Participants 19

Honored Guests 29

List of Company Sponsors 34

v

MEETING ORGANIZATION

Govindan Rangarajan (Chairman)Department of MathematicsIndian Institute of [email protected]

Dipankar Nandi (Convener)Department of BiochemistryIndian Institute of [email protected]

Anjali A. KarandeDepartment of BiochemistryIndian Institute of [email protected]

R. ManjunathDepartment of BiochemistryIndian Institute of [email protected]

Narendra M. DixitDepartment of Chemical EngineeringIndian Institute of [email protected]

Soumyendu RahaSERCIndian Institute of [email protected]

Udaykumar RangaMBGU, [email protected]

Sathees C. RaghavanDepartment of BiochemistryIndian Institute of [email protected]

Deepak K. SainiDepartment of Molecular Reproduction,Development and GeneticsIndian Institute of [email protected]

Parag P. SadhaleDepartment of Microbiology andCell BiologyIndian Institute of [email protected]

Dipshikha ChakravorttyDepartment of Microbiology andCell BiologyIndian Institute of [email protected]

S.G. RamachandraCentral Animal facilityIndian Institute of [email protected]

William R. SurinFACS FacilityIndian Institute of [email protected]

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MESSAGE

We are delighted that a meeting on “Theoretical and Experimental Immunology” isbeing held in the Indian Institute of Science, Bangalore. This meeting is certainly aunique experiment of its kind – but one whose time has probably come! Immunology,in general, is the study of the host defense network against infections, tumors, allergiesetc. Experimental immunology is well studied and has shed light on the inner workingsof immune cells. Studies in this field have led to a better understanding of the hostdefense network. On the other hand, theoretical immunology, utilizing mathematicaltools, is a relatively newer area of study. Theoretical immunology involves thedevelopment of mathematical and computational models of biological phenomenathat unravel, in synergy with experiments, new insights into the workings of our immunesystem. We are happy that this meeting has brought in top notch researchers in thefields of theoretical and experimental immunology.

It is heartening to know that the response to this specialized meeting has been goodwith over 150 student registrations from different parts of the country. Students areencouraged to take the maximum advantage and interact with faculty present in thismeeting. To ensure that students get the maximum advantage, speakers have beenrequested to ensure that the talks are pedagogic in nature. The first half may give theaudience an overview of the field and the second half may focus on the speaker’sresearch contributions. We are hopeful that this meeting will seek answers to questions,which are at the heart of our understanding of Immunology, and may pave the way forthe design of potent adjuvants and vaccines.

This meeting has been possible due to the active participation of several people andinstitutions. We thank IISc for permission to use the various facilities required to conductthis meeting. The support from the Department of Mathematics and the Departmentof Biochemistry, IISc is greatly appreciated. The generous financial assistance fromJNCASR, Centre for Scientific & Industrial Consultancy, IISc and a large number ofprivate companies is greatly appreciated. We thank Carmen Molina-Paris and GrantLythe for suggesting Bangalore to be the venue for this year’s annual meeting of the

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International Network in Theoretical Immunology (INTI). We appreciate the supportfrom other INTI members.

The staff of the IISc Mathematics Initiative (IMI) has played a stellar role in setting upthe web site, taking care of general administrative duties and finances. We thank GrantLythe, Srabanti Rakshit and Mukta Deobagkar for allowing their images to adorn theposters and Abstract book for this meeting. We appreciate the help of EmmanuelStephen and Rajkumar D. from D. Nandi’s laboratory. In addition, several faculty andstudents from the Division of Biological Sciences, IISc have contributed to theorganization of this meeting for which we are extremely thankful.

Finally, we welcome you to this event in Bangalore and look forward to your activeparticipation in making this meeting a resounding success!

With warm wishes,

G. Rangarajan Dipankar NandiChairperson Convenor

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Theoretical and ExperimentalImmunology Meeting (TEIM)

PROGRAM

Faculty HallAug 16, 2011

8:30 am – 9:15 am: Registration

9:15 am Introductory remarks by G. Rangarajan, Chairperson, TEIM

Welcome by D. N. Rao, Divisional Chairman, Division of Biological Sciences, IISc

Vote of thanks by D. Nandi, Convenor, TEIM

9:30 am PLENARY TALKChairperson: G. Rangarajan, Indian Institute of Science, Bangalore

Development and maintenance of the T cell pool in health and diseaseRobin CallardInstitute of Child Health, London, UK

10: 10 am – 10:30 am: TEA

SESSION 1Chairperson: Carmen Molina-Paris, Leeds University

10:30 am – 10:55 am: Studies on the dynamics of germinal centers J. FaroUniversity of Vigo, Spain

10: 55 am – 11: 20 am: Agent based simulation of T cell based immune response: modeling an adoptivetransfer experimentGib BogleUniversity of Auckland, New Zealand

11: 20 am – 11: 45 am: T cells and ageing: effects of age on survival and functionVineeta BalNII, New Delhi, India

Indian Institute of Science,Bangalore

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SESSION IIChairperson: Anjali Karande

11: 45 am – 12:10 pm: Brownian motion and the adaptive immune systemGrant LytheUniversity of Leeds, UK

12: 10 pm – 12: 35 pm: Modelling Cytotoxic T Lymphocyte mediated killing of target cellsSaikrishna GadhamsettyUtrecht University, Netherlands

12: 35 – 1 pm: Potential of gamma delta T lymphocytes for immunotherapy of cancerS. V. ChiplunkarACTREC, Navi Mumbia, India

1:00 – 1: 10 pm: PHOTO (in front of the Tata statue)

1:10 – 2: 25 pm: LUNCH

SESSION IIIChairperson: Grant Lythe, University of Leeds, UK

2: 30 – 2: 55 pm: T cell receptor-ligand binding: mathematical modelingCarmen Molina-ParisUniversity of Leeds, UK

2:55 – 3:20 pm: Mesoscale kinetics models of immunologic reactionsSoumyendu RahaSERC, IISc

3:20 – 3: 45 pm: Macrophage activation - One receptor, two ligands and two pathwaysB. RavindranILS, Bhubaneshwar

3:45 – 4:10 pm: The immunomodulatory role of glycodelin at the feto-maternal interfaceAnjali KarandeIndian Institute of Science, Bangalore, India

4: 10 – 4:30 pm: TEA

SESSION IVChairperson: S. Vijaya, Indian Institute of Science, Bangalore, India

4:30 – 4:55 pm: A systems perspective of host–pathogen interactions: predicting disease outcome intuberculosisNagasuma ChandraIndian Institute of Science, Bangalore, India

4:55 – 5:20 pm: Innate and autoimmune responses induced by chronic human pathogens: lessonsfrom Mycobacteria and Helicobacter pyloriNiyaz AhmedUniversity of Hyderabad, Indiax

5:20 – 5:45 pm: Modulation of immune effectors by mycobacteria: Notch1 signaling paves the wayK. N. BalajiIndian Institute of Science, Bangalore, India

SESSION VChairperson: Ranga Udaykumar, JNCASR, India

5: 45 – 6:10 pm: Threshold surface density of Gp120-CCR5 complexes necessary for HIV-1envelope-mediated cell-cell fusionN. DixitIndian Institute of Science, Bangalore, India

6: 10 – 6:35 pm: Mathematical modeling of anti-retroviral therapy for HIV/AIDS and its impact oncontrolling the spread of virus in the populationArni S.R. Srinivasa RaoIndian Statistical Institute, Kolkata

6: 35 – 7 pm: The current status of HIV-AIDS in IndiaV. RaviNIMHANS

7 pm: HIGH TEA

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1

Theoretical and Experimental Immunology

Development and maintenance of the T cell pool inhealth and disease

Robin CallardInstitute of Child Health, London, UK

The peripheral pool of naive cells is established in children over the first 15-20 years of life and thenremains fairly constant throughout adulthood. The homeostatic mechanisms that maintain the T cellpool in the face of decreasing thymic output, peripheral cell division and loss by differentiation intomemory cells and cell death are now beginning to be understood and can be modelled using ordinarydifferential equations. This modelling approach has been used to explore the host’s homeostaticresponse to lymphopenia caused by HIV infection and shows that the slow loss of T cells leading toAIDS in untreated HIV cannot be simply explained by the dynamics of homeostasis. Rather, slowerosion of lymph node architecture required for homeostatic proliferation may explain the gradualdecline in the homeostatic set point in HIV as well as the relationship to long term immunereconstitution in response to treatment.

2


Studies on the dynamics of germinal centers

J. FaroUniversity of VigoVigo, Spain

Germinal centers (GC) are dynamic, short-lived anatomical structures generated within primary folliclesduring humoral immune responses. Protein antigens (Ag) are actively transported as immune complexesinto B-cell follicles, and ultimately are discharged onto the membrane of the long dendritic arms ofso-called follicular dendritic cells (FDCs). After an initial phase, during which activated Ag-specificT and B cells effectively meet at the B cell zone-T cell zone border and interact intensely, someproliferating B cells migrate back toward the center of a follicle together with some T follicularhelper (Tfh) cells. This initiates the formation of GCs, which are characterized in the first half of theGC reaction by intense B cell proliferation and increasing apoptosis. Currently, it is well establishedthat memory B cell generation and somatic hypermutation take place in GCs. In addition, accumulateddata over the last 15 years clearly indicate that a selection process between Ag-specific B cells, whetherantibody-mutated or not, takes place in GCs, accounting at least partially for affinity maturation.

The population dynamics and transient nature of GCs, as well as the mutation-selection processestaking place there, highlight very interesting problems, amenable to analysis by mathematical andcomputational tools. In this talk I will summarize current key, open questions on GCs as well as somework developed on the question of GC clonal diversity and on possible mechanisms for the rise-and-fall dynamics of the GC reaction.

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Agent-based simulation of T cell immune response:modelling an adoptive transfer experiment

Gib Bogle1, Rod Dunbar2, Evelyn Hyde3, Franca Ronchese4

1Auckland Bioengineering Institute, University of Auckland; Maurice Wilkins Centre, New Zealand2School of Biological Sciences, University of Auckland; Maurice Wilkins Centre3Malaghan Institute4Malaghan Institute, Maurice Wilkins Centre

Many factors are involved in determining the magnitude of an adaptive immune response. The mainreason for developing a simulation model for the T cell immune response in a lymph node is to gaina better understanding of how the various processes act in concert. We are developing an agent-based model that simulates T cell trafficking, motility, interactions with DCs bearing antigen, TCRstimulation, activation and proliferation. This work has revealed many areas where quantitativeinformation is lacking in the literature, and we have begun experiments to fill some of these gaps.Here we report the results of the first experiment, the first attempts to reproduce the experimentalresults by numerical simulation, and interesting issues that this approach revealed: proliferation outsidethe LN greatly exceeds that within the LN, and activated CD8+ T cells exit the LN at a much greaterrate than naïve non-cognate cells.

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T cells and ageing: effects of age on survival andfunction

Vineeta BalNational Institute of Immunology, Aruna Asaf Ali Road,New Delhi 110067, India.

Poor T cell immunity is one of the many defects seen in elderly humans and aged mice. While theindividual organism ages, most of the senescent cells in her body are periodically replaced over aperiod of time. Thus liver shows minimal effect of organismal ageing whereas naive T cells show amajor compromise in their function. One way to explain the difference is that a simple replacementof the liver cell is possible by division of an adjoining cell whereas that of T and B cells is notbecause of the somatic recombinations which take place in the T and B lineage cells after which theyundergo positive and negative selection. Somatic recombination ability provides for the high repertoirediversity and potential to recognise extremly diverse set of antigens. Thus, analysing consequencesof naive T cells which have different T cell receptors and which differ in their individual age becomesan interesting question - different from what happens to other senescent cells in the body.

We have used two approaches to study this issue. In the first approach, we isolate naive CD4 T cellsfrom mice older than 18 months and those 2 months in age and compare their function and survivalin vitro. We find that naive CD4 T [NCD4] cells purified from the spleens of aged mice [ANCD4cells] showed greater apoptosis upon primary activation than those from young mice [YNCD4 cells]early during activation. In the second approach we transfer naive CD4 T cells in congeneic recipientmice and allow donor cells to age for a defined period to analyse the effects. Thus, T cell receptortransgenic [TCR Tg] DO11.10 or OT-2 cells were transferred to congeneic recipients and cells wereallowed to age in vivo upto 6 months. This approach provided us with TCR Tg cells which are morehomogeneous in age and are clonal in nature. We find that more aged TCR Tg cells proliferate poorly,upregulate CD69 less efficiently and their numbers in vivo also decrease with age, an indication of invivo apoptosis. Thus prolonged survival in the absence of specific peptide-MHC ligand based activationsignal appears to make T cells lose their function.

5


Brownian motion and the adaptive immune system

Grant LytheUniversity of Leeds, UK.

I will consider simple models of the movement and interaction of T cells and dendritic cells in alymph node, and analysis of data from in vivo imaging experiments. The adaptive immune responsedepends on T cells coming into physical contact with antigen-presenting cells (APCs) in the T cellzone of one of a lymph node. Recent advances in imaging techniques, especially two-photonmicroscopy, have enabled direct observation of the movement of these labelled cells in the lymphnodes of living mice. One increasingly robust conclusion from analysis of the trajectories of individualcells is that they follow random paths. We will think of a lymphocyte as a particle undergoing Brownianmotion inside a lymph node. Brownian motion is imagined here as the result of many constantly-changing influences that jostle the cell, without any consistently preferred direction, or due to apreference for moving along a network that is randomly arranged. Timescales are expressed in termsof very few parameters: the diffusivity of a cell, the size of the volume that the cell explores, and theradii of the zone of attraction of an APC.

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Modelling cytotoxic T lymphocyte mediated killingof target cells

Saikrishna Gadhamsetty1, Joost Beltman1, Vitaly Ganusov2, Sadna Budhu3,Samuel Silverstein3,4 and Rob de Boer1

1Theoretical Biology and Bioinformatics, Utrecht University, Padualaan 8, 3584CH, Utrecht, The Netherlands.2Department of Microbiology, University of Tennesse, Knoxville TN 37996, USA.3Department of Physiology and Cellular Biophysics,4Department of Medicine,Columbia University College of Physicians and Surgeons, New York, NY 10032, USA.

Cytotoxic T Lymphocytes (CTLs) kill virus-infected and tumor cells, and thus play a crucial roleduring an adaptive immune response. In this study, we analyze a recently published data set of CTLmediated killing of tumor cells in collagen gels, and find that CTLs kill less efficiently at increasedconcentrations. The decrease in killing efficiency already occurs at very low CTL densities and thuscannot be explained by (direct or indirect) interference between CTLs after they have detected targets.Rather, we find that the limitation must lie in the search efficiency of CTLs. Using a simulationmodel of T cell migration, we show that a potential explanation for the decrease in search efficiencycould be the presence of chemo-attraction by tumor cells.

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Potential of gamma delta T lymphocytes forimmunotherapy of cancer

S. V. ChiplunkarAdvanced Centre for Treatment, Research & Education in Cancer,Tata Memorial Centre, Kharghar, Navi Mumbai 410210, India.

Gamma delta T lymphocytes are a distinct subset of lymphocytes that display unique features withrespect to T cell receptor gene usage, tissue tropism and antigen recognition. Aminobisphosphonatesinhibit the farnesyl pyrophosphate synthase (FPPS) enzyme in a dysregulated mevalonate pathway oftumor cells. This leads to elevated levels of intracellular IPP (isopentenyl pyrophosphate) which arerecognised as antigens by Vã9Vδ2 T cells. In the present investigation, attempts have been made tounderstand the molecular mechanism involved in gamma delta T lymphocyte and tumor cell interactionupon treatment with aminobisphosphonates. Studies demonstrated that aminobisphosphonatessensitize tumor cells to augmented lysis by Vã9Vδ2 T cells. Cell cycle analysis of aminobisphosphonatetreated tumor cells revealed a G1/S phase accumulation which may predispose tumor cells to apoptosis.Time lapse video microscopy showed strong conjugate formation between Vã9Vδ2 T cells andtumor cells. Immunonotherapeutic potential of bisphosphonate activated Vã9Vδ2 T cells wereevaluated in vivo in mice xenografted with human breast tumors. After adoptive transfer of Vã9Vδ2T cells increased apoptosis of tumor cells was observed. Vã9Vδ2 T cells also mediate antibodydependant cellular cytotoxicity (ADCC) against tumor cells treated with aminobisphosphonates. Ourstudies elucidate the dual effects of aminobisphosphonates on Vã9Vδ2 T cells and tumor cells andfurther establish its potential for immunotherapy of primary tumors and metastasis.

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T-cell receptor-ligand binding: mathematicalmodelling

Carmen Molina-ParisUniversity of Leeds, UK.

T-cells sense their environment by means of T-cell receptors (TCRs) on their surface. A T-cell expressesabout 30,000 copies of a unique (clonotypic) TCR, whose ligands are complexes composed of apeptide bound to an MHC molecule (pMHC). In vivo, TCR ligands are expressed on the surface ofantigen-presenting cells (APCs). In the thymus a variety of professional APCs will subject immatureT-cells (or thymocytes) to a “double test” by displaying a wide range of pMHC complexes, withpeptides derived from household proteins (self-peptides). The stochastic nature of gene rearrangementsimplies that some TCRs will not be able to recognise a self-pMHC ligand (TCRs that are not functional)and that others will recognise it far too well, and thus would give rise to mature T-cells with thepotential to generate autoimmune responses. Thus, the need for a double test that will check thefunctionality of a thymocyte (positive selection) and its state of tolerance, so that it does not recogniseself-pMHC complexes with high affinities (negative selection). This thymic selection process onlyallows 2-5% of thymocytes to become mature T-cells.

We have made use of mathematical modelling to address the following issues: (1) the thymic affinitythreshold hypothesis proposed by Palmer and Naeher (Nature Reviews Immunology, 2009) and (2)time is precious for T cells, so what do TCRs sense (i) equilibrium properties or (ii) stochastic events.We have made use of data from Palmer’s group (The Journal of Experimental Medicine, 2007) tocompare the equilibrium versus the stochastic hypotheses. Our results indicate that the stochastichypothesis ties in better with the existing immunological evidence and provides support to the affinitythreshold hypothesis. The stochastic model has also been applied to recent two-dimensional bindingdata by Huang et al. (Nature 2010) and sheds light into 2d versus 3d binding kinetics andT-cell responses.

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Mesoscale kinetics models of immunologicreactions

Soumyendu RahaSupercomputer Education and Research CentreIndian Institute of Science, Bangalore 560012, India.

The talk would describe modelling of meso-scale (i.e., medium sized population of reactant speciesmolecules) biochemical kinetics models of reaction networks of immune systems. Physical andmathematical characterization of these models which are chemical Langevin equations and simulationtechniques will be discussed along with examples.

10


Macrophage activation - One receptor, two ligandsand two pathways

B. RavindranInstitute of Life Sciences, Bhubaneswar, India.

Innate immunity to pathogenic organisms is mediated by bacterial/viral and fungal products throughhost Toll Like Receptors that activate macrophages. Endotoxin (LPS) from Gram negative bacteriaactivates macrophages by engaging TLR4 and other cell surface receptors such as MD2 and CD14which results in production of inflammatory mediators such as TNF-α, IL-1β, IL-6 etc. Intracellularpathways involved in this process have been understood significantly in recent years. While searchingfor the elusive innate receptor for nematode pathogens we stumbled on a filarial glycoprotein(designated as AgW) that bound to TLR4. Carbohydrates moieties in AgW were involved in thisinteraction. But unlike LPS, AgW binding to TLR4 resulted in alternate activation of macrophages,a feature characterised by release of anti-inflammatory mediators such as Arginase 1, Chitinases,IL-10 etc., AgW and LPS competitively inhibited each others’ activation pathways. LPS fails to activatemacrophages of C3H/HeJ mice due to a single residue mutation in intracellular domain of TLR4.This mutation also affected alternate activation of macrophages by AgW. This novel feature viz.,LPS and AgW activating macrophages by functionally opposite pathways was used to block LPSmediated endotoxemia both in vitro and in vivo. Chitohexaose (chtx), the carbohydrate residue inhibitedLPS induced inflammatory molecules and protected mortality of experimental mice suggesting thepossibility of using a small molecular weight polysaccharide, chtx as a TLR4 antagonist to blockendotoxemia associated with Sepsis, which is responsible for mortality of about 400,000 humansworldwide.

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The immunomodulatory role of glycodelin at thefeto-maternal interface

Anjali KarandeDepartment of Biochemistry, Indian Institute of Science, Bangalore, India

The fetus is an allograft, half paternal, yet, in a normal pregnancy the fetus stays well protectedbecause of several diverse mechanisms which act in a concerted manner to either suppress fetalallogenicity or inhibit the deleterious effects of the maternal immune response. Of the severalmechanisms that have been reported so far, one is the immunomodulatory activity of a glycoprotein,named glycodelin. Glycodelin is the most abundant progesterone-regulated secretory glycoprotein ofthe endometrium at implantation and during early pregnancy in humans and has been proposed as abiochemical marker of endometrial sufficiency. That glycodelin is indispensable for the establishmentand progression of pregnancy has been well established and its effects on the various cells of theimmune system have been defined to some extent. Glycodelin inhibits the proliferation of activatedT-lymphocyte and also induces apoptosis in them; Glycodelin induces apoptosis in monocytic cellsbefore differentiation, but does not affect their phagocytic capacity after differentiation. Glycodelininhibits B-cell proliferation; Glycodelin inhibits NK cell activity, induces apoptosis in NK cells butthe signaling is independent of caspases. In conclusion, although the type of effect of glycodelin andthe signaling mechanisms involved in each cell type are distinct, the molecule serves to keep thematernal immune response to the fetal allograft in check.

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A systems perspective of host–pathogeninteractions: predicting disease outcome intuberculosis

Nagasuma ChandraDepartment of Biochemistry, Indian Institute of Science,Bangalore - 560 012, India.

The complex web of interactions between the host immune system and the pathogen determines theoutcome of any infection. A computational model of this interaction network, which encodes theintricate interplay among host and bacterial components, forms a useful basis for improving theunderstanding of pathogenesis, in filling knowledge gaps and consequently to identify strategies tocounter the disease. A model of the Mycobacterium tuberculosis host–pathogen interactome, built recentlyin our laboratory, will be presented in this talk. The model contains 75 nodes, about a fourth of themrelating to bacterial components, the rest being components of the human immune system. Thecomplex regulation by the various cytokines present in the cell has also been encoded in the model.Boolean transfer functions describe the relationships between the nodes. Vaccination effects, clearanceefficiencies due to drugs and growth rates have also been encoded in the model. Virtual deletionexperiments were performed, where one or more components of the system were removed and theresponse of the system to this perturbation was analysed. In these simulations, disabling processessuch as phagolysosome fusion or important cytokines such as TNF-α and IFN-γ greatly impairedbacterial clearance, while removing cytokines such as IL-10 alongside bacterial defence proteins suchas SapM greatly favoured bacterial clearance. An interesting result from the simulations is theidentification of the high propensity of the tubercle bacillus to enter a state of ‘persistence’ under avariety of conditions. The model proposed provides insights into TB pathogenesis and also providesa ready framework for integrating a broad range of experimental information as well as quantitativedata at various levels.

13


Innate and autoimmune responses induced bychronic human pathogens: Lessons from myco-bacteria and Helicobacter pylori

Niyaz AhmedPathogen Biology Laboratory, Department of Biotechnology,University of Hyderabad, Hyderabad, India.

Several putative virulence encoding genes have been identified in Mycobacterium tuberculosis and Helicobacterpylori that possibly play crucial roles during chronic persistence of these bacteria and provide themwith survival advantages. We studied the signaling pathways pertaining to proapoptotic and/orproinflammatory behavior of different proteins from H. pylori and pathogenic mycobacteria. In theformer, many of such genes are encoded by the ‘plasticity region cluster’ of the genome and welooked at functions of proteins from this cluster, which are simultaneously proinflammatory andproapoptotic. In M. tuberculosis, we studied a novel member of dos-R family that has both regulatoryand innate immune functions. In addition, we identified a few genes/proteins in M. avium subsp.paratuberculosis (that have homologues also encoded by the human genome) that entail potentialautoimmune responses; these have significant bearing on our understanding of diseases such astype-1 diabetes mellitus, Crohn’s disease and multiple sclerosis.

14


Modulation of immune effectors by mycobacteria:Notch1 signaling paves the way

K. N. BalajiDepartment of Microbiology and Cell Biology,Indian Institute of Science, Bangalore 560012, India.

Pathogenic mycobacteria have evolved unique strategies to survive within the hostile environmentof macrophages. In this intricate process, modulation of immune effectors like SOCS3, COX-2 andMMP-9 acts as an important factor influencing the overall host immune response. Our studydemonstrates that Mycobacterium bovis Bacille Calmette-Guérin (M. bovis BCG) triggered TLR2-dependentsignaling leads to induced expression of SOCS3, COX-2 and MMP-9 in macrophages. Signalingperturbations or genetic approaches suggest signaling integration through cross-talk between Notch1,PI3K during M. bovis BCG triggered expression of multitude of immunological parameters includingSOCS3, COX-2 and MMP-9. Intriguingly, Nitric Oxide assumes critical importance in M. bovis BCGmediated activation of Notch1 signaling as iNOS-/- macrophages exhibited compromised ability toexecute M. bovis BCG triggered Notch1 signaling responses. These findings provide new insightsinto mechanisms by which Notch1, TLR2 and NO signals are integrated in a cross-talk that modulatedefined set of effector functions in macrophages.

15


Threshold surface density of Gp120-CCR5complexes necessary for HIV-1 envelope-mediatedcell-cell fusion

Narendra DixitDepartment of Chemical Engineering,Indian Institute of Science, Bangalore 560012, India.

Binding of the HIV-1 envelope (Env) protein gp120 with CCR5 is essential for the entry ofR5 viruses into target cells. The threshold surface density of gp120-CCR5 complexes that enablesHIV-1 entry remains poorly estimated. We constructed a mathematical model that mimicsEnv-mediated cell-cell fusion assays, where target CD4+CCR5+ cells are exposed to effector cellsexpressing Env in the presence of a coreceptor antagonist and the fraction of target cells fused witheffector cells is measured. Our model employs a reaction network based approach to describe proteininteractions that precede viral entry coupled with the ternary complex model to quantify the allostericinteractions of the coreceptor antagonist and predicts the fraction of target cells fused. By fittingmodel predictions to previously published data of cell-cell fusion in the presence of the CCR5antagonist vicriviroc, we estimated the threshold surface density of gp120-CCR5 complexes forcell-cell fusion. Model predictions with this threshold captured data from independent cell-cell fusionassays in the presence of vicriviroc and rapamycin, a drug that modulates CCR5 expression, as wellas assays in the presence of maraviroc, another CCR5 antagonist, using sixteen different Envclonesderived from transmitted or early founder viruses. Our estimate of the threshold surface density ofgp120-CCR5 complexes necessary for HIV-1 entry thus appears robust and may have implicationsfor optimizing treatment with coreceptor antagonists, understanding the non-pathogenic infectionof non-human primates, and designing vaccines that suppress the availability of target CD4+CCR5+cells.

16


Mathematical modeling of anti-retroviral therapy forHIV/AIDS and its impact on controlling the spreadof virus in the population

Arni S.R. Srinivasa RaoIndian Statistical Institute, Kolkata, India

After the rapid scale up of anti-retroviral therapy (ART) in India, especially during the third phase ofthe National AIDS Control Program (NACP III) (2007-12), there is a need to understand the impactof such therapy on both the dynamics of HIV spread and occurrence of new cases. In this analysisthe experimental evidence available from various cohorts of individuals who were recruited for ARTis blended with dynamical models specifically developed for studying the impact of ART on thepopulation. These models essentially consist of sets of integral-differential equations and a combinationof conditional probabilities. ART, in combination with prevention measures, has contributed to thereduction of HIV burden in several countries. In this talk, we present the methods and model developedfor tracing the impact of ART in India during the third round of National AIDS Control Program(2007-2012). We highlight the concepts of ‘halting’ and ‘reversal’ of the epidemic and try to build atheory involving these concepts. Projected ART impact and projected HIV during the next phase ofthe NACP (2012-2017) are discussed. We further studied stability of the system. We have conductedsensitivity analysis of the level of ART intervention on the program results. The analysis led to newresults on transition theory pertaining to the concepts of ‘halting’ and ‘reversal’. This talk is based onthe joint working paper listed in the reference.

ReferenceRao, Arni S.R. Srinivasa, Thomas Kurien, Sudhakar Kurapati, Bhat Ramesh and Maini, P.K. (2011).Working Paper in progress (63 pages). Earlier version of the draft paper was prepared as an input forpreparatory process for the Fourth Phase of National AIDS Control Program, NACP-IV (2012-17),National AIDS Control organization (NACO), Department of AIDS Control, Ministry of Healthand Family Welfare, Government of India.

17


The current status of HIV-AIDS in India

V. RaviNIMHANS, Bangalore, India

18


LIST OF SPEAKERS

Niyaz AhmedLS-296, School of Life Sciences,University of Hyderabad, Gachibowli,Hyderabad 500046, IndiaEmail: [email protected]

Vineeta BalNational Institute of Immunology,Aruna Asaf Ali Marg,New Delhi 110 067, India.Email: [email protected]

K. N. BalajiDept. of Microbiology & Cell BiologyIndian Institute of ScienceBangalore-560012, IndiaEmail: [email protected]

Gib BogleSenior Research FellowAuckland Bioengineering InstituteUniversity of Auckland, New ZealandEmail: [email protected]

Robin CallardProfessor, Institute of Child HealthUniversity College London30 Guilford st, London WC1N 1EH, UK

Nagasuma ChandraAssociate ProfessorDept. of BiochemsitryIndian Institute of Science,Bangalore - 560012, IndiaE-mail: [email protected],[email protected]

S. V. ChiplunkarACTREC, Tata Memorial Centre (TMC)Sector-22, KhargharNavi Mumbai, 410 210, IndiaEmail: [email protected]

Narendra M. DixitDepartment of Chemical EngineeringIndian Institute of Science,Bangalore - 560 012, IndiaEmail: [email protected]

Jose FaroUniversity of VigoDepartment of Biochemistry,Genetics and Immunology

Edificio de Ciencias ExperimentalesCampus As Lagoas-Marcosende36310 Vigo, SpainEmail: [email protected]

Saikrishna GadhamsettyZ529, Theoretical Biology and BioinformaticsUtrecht University, Padualaan 8, 3584 CHUtrecht, The NetherlandsEmail: [email protected],[email protected]

Anjali KarandeDepartment of BiochemistryIndian Institute of ScienceBangalore-560 012, IndiaEmail: [email protected]

Grant LytheReader in Applied MathematicsUniversity of Leeds, Leeds LS29JT, UKEmail: [email protected]

Carmen Molina-ParisProfessor in Applied MathematicsUniversity of Leeds, Leeds LS2 9JT, UKEmail: [email protected]

Soumyendu RahaSERCIndian Institute of Science,Bangalore - 560 012, IndiaEmail: [email protected]

B. RavindranDirector, Institute of Life Sciences,(Department of Biotechnology)Nalco Square, Bhubaneswar 751023, IndiaEmail: [email protected],[email protected]

V. RaviProfessor and HeadDept. of NeurovirologyNIMHANS, Bangalore – 560029, IndiaEmail: [email protected]

Arni S.R. Srinivasa RaoB.I.R.U., 4th Floor, R.A. Fisher BuildingIndian Statistical Institute203 B.T. Road, Kolkata 700108, IndiaEmail: [email protected]

19


LIST OF PARTICIPANTS

1. Akula Srinivas3-113, Vadapally, DamacherllaNalgunda District, AndhraEmail: [email protected]

2. Ashutosh KumarLS-273, Dept of Biotechnology,University of Hyderabad,HyderabadEmail: [email protected]

3. B Hemanth NayakRoom No. 51, ‘B’ Block,Boys Hostel, SV University,Tirupathi, Chittoor District,Andhra PradeshEmail: [email protected]

4. Bandari SreevaniH. No. 81/155, Parvathi Nagar,Kurnool District,Andhra PradeshEmail: [email protected]

5. Bhagchand GurjarV230, Vashistha Hostel,SASTRA University, ThanjavurEmail: [email protected]

6. Bhairav PalejaKS-262, Chiplunkar Lab, KhanolkarShodhika, ACTREC, Tata MemorialCentre, Kharghar, Navi MumbaiEmail: [email protected]

7. Byreddy AlekhyaDoor No. 11/37, PTM Road,B. Kotha Kota, Chitttoor Disitrict,Andhra PradeshEmail: [email protected]

8. Dodla SumathiKammapalem Village,Kodavaluru Mandal,Nellore District, Andhra PradeshEmail: [email protected]

9. Dollu Srichakra80/112 G 2-A-1, Setlams Nilayam,Subhash Nagar, Kurnool District,Andhra PradeshEmail: [email protected]

10. G Muni Swamy, M.Sc.Immuno Technology,Dept. of Bio Chemistry,Srivenkateswara University, TirupatiEmail: [email protected]

11. Gandreti Santhosh Krishna RaoKothaboddam, Boddam Post,Vepada Mandal, Viziyanagaram,Andhra PradeshEmail: [email protected]

12. Harman Preet Kaur DhillonD/o Harjinder Singh Dhillon,Giri Road, Hillpatna, Berhampur,Dist. Ganjam, OrissaEmail: [email protected]

13. J Cyril Jones10/2, Diwan Bhashyam Street,West saidapet, ChennaiEmail: [email protected]

14. J Sudheer KumarH. No: 50-348-M-3-1, Arora Nagar,B-Camp, Kurnool District,Andhra PradeshEmail: [email protected]

15. K V KhajapeerDoor No: 19-4-123/8/B, S.T.V. Nagar,Tirupathi, Chittoor District,Andhra PradeshEmail: [email protected]

16. Kishore Babu10-1-707, Surendra NagarBadvel(m), Kadapa, Andhra PradeshEmail: [email protected]

Outstation Participants for the “International Meeting on Theoretical andExperimental Immunology” on August 16, 2011

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17. Kishore NalamPathogen Biology Laboratory,Dept of Biotechnology, School of LifeSciences, University of HyderabadEmail: [email protected]

18. Koustava Kumar PandaC/o Satpal Singh Bisht, Prof. & AMP;Head, Dept. of BiotechnologyRoland Institute of PharmaceuticalSciences, Gopalpur Junction, Berhampur,Ganjam, OrissaEmail: [email protected]

19. Kumar MohitPrist UniversityEmail: [email protected]

20. Maitreyee SharmaC/o H.O.D. Dept. of MBBT,Tezpur University, Tezpur,Napaam, AssamEmail: [email protected]

21. Mohammed MajidLS-273, Pathogen Biology Lab,Dept. of Biotechnology,University of Hyderabad,Hyderabad, Andhra PradeshEmail: [email protected]

22. Pasupuleti DhanamjayuluHouse No. 8-50/3/a, Before RevandsFactory, Chandragiri, Tirupathi,Chittoor District, Andhra PradeshEmail: [email protected]

23. Poornemaa .N‘B’ Tulsi Nivas, 21/69, Canal Bank RoadEast, R.A. Puram, ChennaiEmail: [email protected]

24. Pratheek BMSchool of Biological SciencesNational Institute of Science Education &Research, Institute of Physics Campus,P.O: Sainik School,Bhubaneswar, OrissaEmail: [email protected]

25. Praveen BoddanaC/o S.P.S. Bisht, Roland Institute ofPharmaceutical Sciences,Berhampur, OrissaEmail: [email protected]

26. Preethi Narayani T RNew No. 31, Old No. 9, Subbu Street,Thiruvanmiyur, ChennaiEmail: [email protected]

27. Pushpa SinghD/o A.K. Singh, Qtr No. 1721,H.A.L Township, Ojhar Nasik,MaharashtraEmail: [email protected]

28. Puttareddy Divyamrita4-20, Sri Krishna Nagar, M.R. Palli,Tirupathi, Chittoor District,Andhra [email protected]

29. R. K. Anantha Seetha29/3, Sugar Mill Colony,TirunelvelirkEmail: [email protected]

30. Ram Kumar2/404,V.O.C Nagar,Belathur Post, HosurEmail: [email protected]

31. Rubia L askerSadar para, Jitu Mistry Lane,Purulia (723101), West BengalEmail: [email protected]

32. S. VenkateswarluHouse No: 8-17-127/52/1a/1,Nallabanda Street, Giddalur Mandal,Prakasam District, Andhra PradeshEmail: [email protected]

33. Sangram Keshari SinghRaveswaya Park, Gunjan,Vapi, GujraratEmail: [email protected]

21


34. Sankar. KB-Block, Room No. 35,SVU College of Science, Men Hostels,Sri Venkateswara University,TirupathiEmail: [email protected]

35. SanthiC/o Alamelu Raja, Dept of Immunology,Tuberculosis Researchcenter,Chetpet, ChennaiEmail: [email protected]

36. Savita DeviRoom No: LS-273,Pathogen Biology Laboratory,Department of Biotechnology,School of Life Sciences,University of Hyderabad, HyderabadEmail: [email protected]

37. Singam Chaithanya KumariH. No: 57/19, Chidambara Rao Street,Kurnool District, Andhra PradeshEmail: [email protected]

38. Sneha S139/17 Kailash Colony, Annanagar WestExtnsion, ChennaiEmail: [email protected]

39. Sowmya. RNo. 32 Montieth Mansion, Montieth Road,Egmore, ChennaiEmail: [email protected]

40. Subramanyam DasariResearch ScholarC/o. Prof. W. RajendraDept.Of Zoology,S.V. University, TirupatiEmail: [email protected]

41. Tarugu Diwakar ReddyMSc (Biochemistry) StudentDept.of Biochemistry,S.V. Universuty, TirupatiEmail: [email protected]

42. Valathati Stella SravanthiPlot No: 24, SV Badra Residency,Balaji Hills Colony, Near Gayatri Gardens,Nizampeta Village, Hyderabad,Andhra PradeshEmail: [email protected]

43. Veeram SindhujaH. No. 15-9H-5-6,Government Hospital Road,Near New Maszid, Nandikotkur,Kurnool District, Andhra PradeshEmail: [email protected]

44. Vidya RanganathanImmunobiology Lab I, C/o Vineeta Bal,National Institute of Immunology,Aruna Asaf Ali Marg, New DelhiEmail: [email protected]

45. Y KarthikDoor No: 202, B Block, Surya Towers,Dharmapet, Kurnool District,Andhra PradeshE-mail: [email protected]

46. Z. Md. WasimhNo. 8/64c, Maldarpet, Nandyal,Kurnool District, Andhra PradeshEmail: [email protected]

47. Vani Janakiraman46c, Shanthi Nagar - West MainHosur 635109, Krishnagiri Dist,Tamil NaduEmail: [email protected]

48. Harsha. S. N323, HPO & amp; RMS layout,Shakti Nagar, MysoreEmail: [email protected]

49. A Sainath ReddyBijiwar, Maldakal, MahabuanagarEmail: [email protected]

50. AnuradhaDepartment of ImmunopathologyPGIMER, ChandigarhEmail: [email protected]

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51. B SubhasiniGodugunuru, Badvel, YSR District,Andhra PradeshEmail: [email protected]

52. G DevikaOm Shanthi Nagar,Kadapa District, Andhra PradeshEmail: [email protected]

53. G Lakshmi DeviH. No: 16/48, Kovelakunta,Kurnool District, Andhra [email protected]

54. G R Bhanu PrakashRoom No: 51 ‘B’ Block Anjandri,SVU Campus, TirupathiEmail: [email protected]

55. Giri KumarState Bank Lane, Addanki, Prakasam Dist.Email: [email protected]

56. K NarasappaRoom No: 35, B Block Anjanadri,SVU Campus, SV University, TirupathiEmail: [email protected]

57. K PadmajameenakshiGonegandla, Kurnool District,Andhra PradeshEmail: [email protected]

58. Kanala Vijaya KumarD. No: 1-477-1, Santi Nagar,Dharmavarm, Anatapur DistrictEmail: [email protected]

59. Mopuri RamgopalC/o M. Balaji, Dept. of Biochemistry,S.V. University, TirupatiEmail: [email protected]

60. P BhaskarRoom No: 51, ‘B’ Block Anajandri,SVU Campus, TirupathiEmail: [email protected]

61. P. Sandhya RaniLS-273, Pathogen Biology Lab,Department of Biotechnology,University of Hyderabad, HyderabadEmail: [email protected]

62. S Mahaboob Basha4/73, C.C. Kothakota,Pothulangepalli,Dharmavarm, AnatpurEmail: [email protected]

63. S ShabeerMerugudoddy, Burandoddy,Kodumur, Kurnool DistrictEmail: [email protected]

64. Thandlam Mahesh KumarDoor No: 343, Balaji Nagar,Tirumala, Chittoor District,Andhra PradeshEmail: [email protected]

65. V VaralakshmiD No : 9/194, K. Bhaskarpet,Mylavaram, Kadapa District,Andhra PradeshEmail: [email protected]

66. Bhushan S. PatilIndustrail Biotechnology,Sastra University, ThanjavurEmail: [email protected]

67. M. Suvarsha RaoCCMB, HyderabadEmail: [email protected]

23


LIST OF PARTICIPANTS

Department of Biochemistry

1. Ajay Kumar DixitC/o Prof. JayabaskaranDept. of Biochemistry,Indian Institute of Science, BangaloreEmail: [email protected]

2. Arvind AThe Lab of P. N. Rangarajan,Department of Biochemistry,Indian Institute of Science, BangaloreEmail: [email protected]

3. B. ChetanaC/o Prof. Dipankar Nandi,Department of Biochemistry,Indian Institute of Science, BangaloreEmail: [email protected]

4. Chaitanya DendeDept of BiochemistryProf. P.N. Rangarajan,Indian Institute of Science, BangaloreEmail: [email protected]

5. Divya BhardwajC/o Prof Anjali A KarandeDept of BiochemistryIndian Institute of Science, BangaloreEmail: [email protected]

6. Emmanuel Stephen V# 22 LIG, HUDCOUdayagiri, MysoreEmail: [email protected]

7. IyappanDepartment of Biochemistry,Indian Institute of Science, BangaloreEmail: [email protected]

8. K. PrashanthiC/o Dr Nagasuma ChandraDept of BiochemistryIndian Institute of Science, BangaloreEmail: [email protected]

9. Manoj BhosaleC/o Dr Dipankar NandiDept of Biochemistry,Indian Institute of Science, BangaloreEmail: [email protected]

10. Mohit AroraD.N. Rao Lab, Dept. Of Biochemistry,Indian Institute of Science, BangaloreEmail: [email protected]

11. Monali ManoharProf. Anjali Karande’s Laboratory,Dept. of Biochemistry,Indian Institute of Science, BangaloreEmail: [email protected]

12. Mrinmoy DasC/o Prof. Dipankar NandiDept of Biochemistry,Indian Institute of Science, BangaloreEmail: [email protected]

13. Mukta DeobagkarC/o Prof. Dipankar Nandi,Dept of Biochemistry,Indian Institute of Science, BangaloreEmail: [email protected]

14. Nishana MC/o Dr Sathees C. Raghavan,Dept. of Biochemistry,Indian Institute of Science, BangaloreEmail: [email protected]

15. Piyush Kumar SinghAnjali Karande’s Lab,Dept. of Biochemistry,Indian Institute of Science, BangaloreEmail: [email protected]

16. Ritu MishraC/o Prof. Anjali Karande,Dept. of Biotechnology,Indian Institute of Science, BangaloreEmail: [email protected]

Local Participants for the " International Meeting on Theoretical andExperimental Immunology" on August 16, 2011

24


17. Rohit VashishtC/o Prof. Nagasuma Chandra,Department of Biochemistry,Indian Institute of Science, BangaloreEmail: [email protected]

18. Rubeena. MDipankar Nandi’s Lab,Department of Biochemistry,Indian Institute of Science, BangaloreEmail: [email protected]

19. ShwetankDept. of Biochemistry,Indian Institute of Science, BangaloreEmail: [email protected]

20. Soma DasPost Doctoral Fellow,C/o Prof. Anjali A. Karande,Dept. of Biochemistry,Indian Institute of Science, BangaloreEmail: [email protected]

21. Srabanti RakshitDepartment of Biochemistry,Indian Institute of Science, BangaloreEmail: [email protected]

22. SudarshanC/o Prof. Anjali Karande,Department of Biochemistry,Indian Institute of Science, BangaloreEmail: [email protected]

23. TanushreeC/o Prof. Dipankar Nandi,Dept. of Biochemistry,Indian Institute of Science, BangaloreEmail: [email protected]

24. Bhagawat ChandrasekarC/o Prof. Dipankar Nandi,Department of Biochemistry,Indian Institute of Science, BangaloreEmail: [email protected]

25. Rananaware Supriya RajendraDepartment of Biochemistry,Indian Institute of Science, BangaloreEmail: [email protected]

26. Rupa KumariC/o Sathees C. Raghavan,Dept. of Biochemistry,Indian Institute of Science, BangaloreEmail: [email protected]

27. Rajkumar .D28/10, 2nd Main, Attigupee,Vijayanagar, BangaloreEmail: [email protected]

Department of Microbiology andCell Biology

1. Akhauri Yash SinhaK.N Balaji Lab, Dept of MCBL,Indian Institute of Science, BangaloreEmail: [email protected]

2. Anish R VC/o Dipshika Chakravortty,CIDR, MCBL,Indian Institute of Science, BangaloreEmail: [email protected]

3. Debalina ChaudhuriC/o Dipshikha Chakrovortty,Dept of Microbiology and Cell Biology,Indian Institute of Science, BangaloreEmail: [email protected]

4. Devram Sampat GhorpadeC/o K. N. Balaji, Dept. of Microbiologyand Cell Microbiology,Indian Institute of Science, BangaloreEmail: [email protected]

5. Jamma TrinathLaboratory of K.N. Balaji,Department of Microbiology andCell Biology,Indian Institute of Science, BangaloreEmail: [email protected]

6. Kannan B NC/o Prof. Parag Sadhale,Dept of Micorbiology and Cell Biology,Indian Institute of Science, BangaloreEmail: [email protected]

25


7. Kavya AnanthaswamyFACS Facility, Ground Floor,CIDR Building, Dept. of Microbiologyand Cell Biology,Indian Institute of Science, BangaloreaEmail: [email protected]

8. Madhura JoglekarC/o Dr Dipshikha Chakravortty,Dept of MCB, Indian Institute of Science,BangaloreEmail: [email protected]

9. Namrata IyerC/o Dipshikha Chakravortty,Department of Microbiology andCell Biology, Indian Institute of Science,BangaloreEmail: [email protected]

10. Pallavi KakadeRoom No. 115, Dept of Microbiology andCell Biology, Indian Institute of Science,BangaloreEmail: [email protected]

11. Prasanna BhatLab No. 252, Dept of Microbiology andCell Biology, Indian Institute of Science,BangaloreEmail: [email protected]

12. Preeti GaraiC/o Dipshikha ChakrvorttyDept. of Microbiology and Cell Biology,Indian Institute of Science, BangaloreEmail: [email protected]

13. Puja PaiFACS Facility, Dept. of Microbiology andCell Biology, Indian Institute of Science,BangaloreEmail: [email protected]

14. Ravinayak PathlavathC/o K.N. Balaji, Dept. of Microbiologyand Cell Biology, Indian Institute ofScience, BangaloreEmail: [email protected]

15. Sahana HollaC/o K. N. Balaji, 208,Dept. of Microbiology and Cell BiologyIndian Institute of Science, BangaloreEmail: [email protected]

16. Sai Rama Krishna MekaC/o Dipshika Chakraborthy,Molecular Pathogenesis Lab,Dept. of Micro Biology & Cell Biology,Indian Institute of Science, BangaloreEmail: [email protected]

17. SandhyaC/o Dipshikha Chakravortty,Dept. of Micro Biology & Cell Biology,Indian Institute of Science, BangaloreEmail: [email protected]

18. SangeetaC/o Dr DipshikhaDept. of Micro Biology andCell Biology (CIDR),Indian Institute of Science, BangaloreEmail: [email protected]

19. Sanjay Kumar. PSubba Rao Lab, Microbiology and CellBiology, Indian Institute of Science,BangaloreEmail: [email protected]

20. Saurabh KumarC/o Prof. S Vijaya, Room No 254,Dept of Microbiology and Cell Biology,Indian Institute of Science, BangaloreEmail: [email protected]

21. Shakuntala PillaiC/o K.N. Balaji, Dept. of Microbiologyand cell Biology,Indian Institute of Science, BangaloreEmail: [email protected]

22. Shambhuprasad. T.K.C/o K.N. Balaji Lab, Dept. ofMicrobiology and cell Biology,Indian Institute of Science, BangaloreEmail: [email protected]

26


23. ShwethaProf. Saumitra Das’s LabDept. of Microbiology and cell Biology,Indian Institute of Science, BangaloreEmail: [email protected]

24. ShobhaEB-650, New Housing Colony,Indian Institute Of Science, BangaloreEmail: [email protected]

25. Thomas PaulrajC/o K.N. Balaji, Dept. ofMicrobiology and cell Biology,Indian Institute of Science, BangaloreEmail: [email protected]

26. Divya Prakash GC/o Dr. Dipshikha Chakravortty,Centre for Infectious Disease Research(CIDR), Indian Institute of Science,BangaloreEmail: [email protected]

Molecular Biophysics Unit

1. Kirtimaan SyalProf. D. Chatterji Lab,Molecular Biophysics Unit,Indian Institute of Science, BangaloreEmail: [email protected]

2. Subho GhoshMolecular Biophysics Unit,Indian Institute of Science, BangaloreEmail: [email protected]

3. V Vamsee Aditya MallajosyulaLab # 202, Molecular Biophysics Unit,Indian Institute of Science, BangaloreEmail: [email protected]

4. Priyanka BaloniC/o Prof. Nagasuma ChandraBioinfromatics centre, Raman building,Indian Institute of Science, BangaloreEmail: [email protected]

5. Awanti SambareyC/o Dr Nagasuma Chandra,Indian Institute of Science, BangaloreEmail: [email protected]

6. Sannula KesavardanaC/o Prof. Raghavan Varadarajan’s Lab,Molecular Biophysics Unit,Indian Institute of Science, BangaloreEmail: [email protected]

7. Sumanta MukherjeeDept of Mathematics, (IMI office)Indian Institute of Science,BangaloreEmail: [email protected]

IISc Students

1. AparnaC/o Dr. Upendra Nongthomba,Dept. of MRDG, Indian Institute ofScience, BangaloreEmail: [email protected]

2. Arunita ChatterjeeC/o Dr. Upendra Nongthomba,Dept. of MRDG, Indian Institute ofScience, BangaloreEmail: [email protected]

3. Shalu Verma-KumarCentre for Ecological Sciences,Indian Institute of Science,Bangalore-560012 &C/o Dr. K.N. Balaji, Microbiology andCell Biology, Indian Institute of Science,BangaloreEmail: [email protected]

4. Anurag MisraDept. of Physics, Indian Institute ofScience, BangaloreEmail: [email protected]

5. Vidya DeviSA22 Aryabhatta Aptt. Indian Institute ofScience Campus, BangaloreEmail: [email protected]

27


6. Shiva NareshLab-1, Dept. of Chemical Engineering,Indian Institute of Science, BangaloreEmail: [email protected]

7. Supriya VartakLRG3 Rohini Hostel,Indian Institute of Science Campus,BangaloreEmail: [email protected]

8. Saswati Dana#18, Paresh Apartments, 4th Main,13th Cross, Malleswaram,BangaloreEmail: [email protected]

9. Ruchi AgrawalMRDG, Indian Institute of Science,BangaloreEmail: [email protected]

10. Ruchi JainMRDG, Indian Institute of Science,BangaloreEmail: [email protected]

11. Raji NairMRDG, Indian Institute of Science,BangaloreEmail: [email protected]

12. Mayur RajankarMRDG, Indian Institute of Science,BangaloreEmail: [email protected]

Local Participants from Bangalore

1. Vikas AgarwalSrinivas 2nd Cross, 2nd Main,Hulimavu Gate, Thimmappa ReddyLayout, Bannerghatta Road,BangaloreEmail: [email protected]

2. Shivayogi MSSmarth Biologicals Pvt. Ltd.Main Peenya Industrial Area,First Phase, BangaloreEmail: [email protected]

3. Smitha SharmaSamarth Life Sciences, #41-p3,3rd Main Road, 1st Phase PeenyaIndustrial Area, BangaloreEmail: [email protected]

4. Mahima Arora#1/1, M. Appanna Mansion,Kamala Kuteera Girls, PG,Thavarekere Main Road,Near Federal Bank ATM,Thavarekere, BangaloreEmail: [email protected]

5. Abhishek K. SrivastavaSyngene International Ltd,Biocon Park, BBRC Build. S11,Plot 2&3, Jigani Link Road,Bommasandra Phase IV, BangaloreEmail: [email protected]

6. Swarupa YallaMBGU, JNCASR, Jakkur Post,BangaloreEmail: [email protected]

7. Prabhu S AHIV - AIDS Laboratory,MBGU, JNCASR, Jakkur P.O.,BangaloreEmail: [email protected]

8. ShilpeeHIV-AIDS Lab, MBGU, JNCASRJakkur, BangaloreEmail: [email protected]

9. Supriya. R#74, 3rd Main, Nagendra Block,SBM Colony, BangaloreEmail: [email protected]

10. Meenakshi PB1, Teresa Towers, 10th ‘A’ Cross,16th Main, Near Mico Layout,Police Station, BTM Layout,2nd Stage BangaloreEmail: [email protected]

11. Setareh farzanehkari168/21 4th Cross, SG Payla,Hosur Road, BangloreEmail: [email protected]

28


12. Vignesh Subramanian#101, Jayalakshmi Apartments,Krishna Reddy Colony,Domlur Layout, BangaloreEmail: [email protected]

13. Pranav RKottakkal Arya Vaidya Sala,120/3, 6th ‘C’ Main, 30th Cross,4th, Block, Jayanagar, BangaloreEmail: [email protected]

14. Princy KhuranaMaria Bhavan PG for Ladies,#89/7, 1st Main, Thavarekere Main Road,S. Gpalya, D.R.C Post, Opposite oracle,BangaloreEmail: [email protected]

15. Atmeeya#117,Vakil Garden City,Off Kanakpura Road,Near Thallaghattapura, BangaloreEmail: [email protected]

16. Asha Soman# 442, IIM Bangalore,Bannerghatta Road,BangaloreEmail: [email protected]

17. K.S. Shabnam#1096 7th ‘A’ Main, BTM Layout1st Stage Bannergatta Cross Road,BangaloreEmail: [email protected]

18. Shailesh DudhgaonkarS11, BBRC, Biocon Park,Jigni-Link Road,Bommasandra Industreal area,BangaloreEmail: [email protected]

19. Abhishek. JNo. 1281, 6th Main, 1st ‘A’ Block,2nd Stage, Rajajinagar, BangaloreEmail: [email protected]

20. Anjali VermaHIV-AIDS Lab, MBGU, JNCASRBangaloreEmail: [email protected]

21. Mahesh Kumar MC/o. R.S Jayshree, Prof. and Head,Department of Microbiology,Kidwai Memorial Institute of Oncology,Hosur road, BangaloreEmail: [email protected]

29


LIST OF HONORED GUESTS

G. PadmanabanDepartment of BiochemistryIndian Institute of [email protected]

H. S. SavithriDepartment of BiochemistryIndian Institute of [email protected]

D. Narasimha RaoDepartment of BiochemistryIndian Institute of [email protected]

K. MuniyappaDepartment of BiochemistryIndian Institute of [email protected]

C. JayabaskaranDepartment of BiochemistryIndian Institute of [email protected]

Ram RajasekharanDepartment of BiochemistryIndian Institute of [email protected]

P. N. RangarajanDepartment of BiochemistryIndian Institute of [email protected]

Utpal S. TatuDepartment of BiochemistryIndian Institute of [email protected]

Shikha LalorayaDepartment of BiochemistryIndian Institute of [email protected]

Patrick D' SilvaDepartment of BiochemistryIndian Institute of [email protected]

Ganesh NagarajuDepartment of BiochemistryIndian Institute of [email protected]

A. Jagannadha RaoDepartment of BiochemistryIndian Institute of [email protected]

Appaji RaoDepartment of BiochemistryIndian Institute of Science

S. K. PoddarDepartment of BiochemistryIndian Institute of Science

T. RamasarmaDepartment of BiochemistryIndian Institute of Science

R. MaheshwariDepartment of BiochemistryIndian Institute of Science

P. G. VatsalaDepartment of BiochemistryIndian Institute of [email protected]

K. P. GopinathanDepartment of Microbiology andCell BiologyIndian Institute of [email protected]

H. G. Sharat ChandraDepartment of Microbiology andCell BiologyIndian Institute of [email protected]

M. S. ShailaDepartment of Microbiology andCell BiologyIndian Institute of [email protected]

30


V. NagarajaDepartment of Microbiology andCell Biology, Indian Institute of [email protected]

Umesh VarshneyDepartment of Microbiology andCell BiologyIndian Institute of [email protected]

C. Durga RaoDepartment of Microbiology andCell BiologyIndian Institute of [email protected]

Usha VijayraghavanDepartment of Microbiology andCell BiologyIndian Institute of [email protected]

Utpal NathDepartment of Microbiology andCell BiologyIndian Institute of [email protected]

K. SomasundaramDepartment of Microbiology andCell BiologyIndian Institute of [email protected]

G. Subba RaoDepartment of Microbiology andCell BiologyIndian Institute of [email protected]

Saumitra DasDepartment of Microbiology andCell BiologyIndian Institute of [email protected]

S. VijayaDepartment of Microbiology andCell BiologyIndian Institute of [email protected]

P. AjitkumarDepartment of Microbiology andCell BiologyIndian Institute of [email protected]

S. S. IndiDepartment of Microbiology andCell BiologyIndian Institute of [email protected]

Mrinal GhoshDepartment of MathematicsIndian Institute of [email protected]

S. UmapathyDepartment of Inorganic andPhysical ChemistryIndian Institute of [email protected]

P. BalaramMolecular Biophysics UnitIndian Institute of [email protected]

M. VijayanMolecular Biophysics UnitIndian Institute of [email protected]

Dipankar ChatterjiMolecular Biophysics UnitIndian Institute of [email protected]

A. SuroliaMolecular Biophysics UnitIndian Institute of [email protected]

S. K. SikdarMolecular Biophysics UnitIndian Institute of [email protected]

Raghavan VaradarajanMolecular Biophysics UnitIndian Institute of [email protected]

31


N. SrinivasanMolecular Biophysics UnitIndian Institute of [email protected]

B. GopalMolecular Biophysics UnitIndian Institute of [email protected]

Vidyanand NanjundiahDepartment of Molecular Reproduction,Development & GeneticsIndian Institute of [email protected]

S. MahadevanDepartment of Molecular Reproduction,Development & GeneticsIndian Institute of [email protected]

Upendra NongthombaDepartment of Molecular Reproduction,Development & GeneticsIndian Institute of [email protected]

Arun KumarDepartment of Molecular Reproduction,Development & GeneticsIndian Institute of [email protected]

P. B. SeshagiriDepartment of Molecular Reproduction,Development & GeneticsIndian Institute of [email protected]

R.M. MedhamurthyDepartment of Molecular Reproduction,Development & GeneticsIndian Institute of [email protected]

Sandhya S. VisweswariahDepartment of Molecular Reproduction,Development & GeneticsIndian Institute of [email protected]

Paturu KondaiahDepartment of Molecular Reproduction,Development & GeneticsIndian Institute of [email protected]

Rajan R. DigheDepartment of Molecular Reproduction,Development & GeneticsIndian Institute of [email protected]

Annapoorni RangarajanDepartment of Molecular Reproduction,Development & GeneticsIndian Institute of [email protected]

Vijayalakshmi RavindranathCentre for NeuroscienceIndian Institute of [email protected]

Aditya MurthyCentre for NeuroscienceIndian Institute of [email protected]

Shyamala ManiCentre for NeuroscienceIndian Institute of [email protected]

K.R. ShivakumarCentral Animal FacilityIndian Institute of [email protected]

Rosa SamuelCentral Animal FacilityIndian Institute of [email protected]

Omana JoyFACS [email protected]

R. SukumarCentre for Ecological SciencesIndian Institute of [email protected]

32


Praveen KaranthCentre for Ecological SciencesIndian Institute of [email protected]

Renee M. BorgesCentre for Ecological SciencesIndian Institute of [email protected]

Rohini BalakrishnanCentre for Ecological SciencesIndian Institute of [email protected]

Sushama YermalUG ProgramIndian Institute of [email protected]

Sanjukta ChatterjiUG ProgramIndian Institute of [email protected]

Kayvan ZainabadiUG ProgramIndian Institute of [email protected]

Rabindranath NayakSchool of Biological Sciences (NISER)[email protected]

Prof. Subba RaoBigtech LabsBangalore

Apurva [email protected]

M. R. S. [email protected]

Chandrabhas [email protected]

Anuranjan AnandMolecular Biology & [email protected]

Tapas K. KunduMolecular Biology & [email protected]

Kaustuv SanyalMolecular Biology & [email protected]

Namita SuroliaMolecular Biology & [email protected]

Hemalatha BalaramMolecular Biology & [email protected]

Maneesha InamdarMolecular Biology & [email protected]

Ravi ManjithayaMolecular Biology & [email protected]

Jayachandra A NAdministrative [email protected]

Chiranjib BhattacharyyaComputer Science and Automation,Indian Institute of [email protected]

Siddhartha GadgilDepartment of MathematicsIndian Institute of [email protected]

33


G. K. Anantha SureshMechanical EngineeringIndian Institute of [email protected]

Debnath PalSERC, Indian Institute of [email protected]

Vijay NatarajanComputer Science and AutomationIndian Institute of [email protected]

A. K. NandakumaranDepartment of Mathematics,Indian Institute of [email protected]

A. G. MenonDept. of InstrumentationIndian Institute of [email protected]

Prabal K MaitiDepartment of Physics,Indian Institute of [email protected]

A. M. UmarjiMaterial Research Centre,Indian Institute of [email protected]

Bikramjit BasuMaterial Research Centre,Indian Institute of [email protected]

Satyajit MayorNCBS, [email protected]

P. S. Anil KumarDepartment of Physics,Indian Institute of [email protected]

ChairmanCSICIndian Institute of Science

34


LIST OF SPONSORS

1. Jawaharlal Centre for AdvnacedScientific Research (JNCASR)Jakkur P.O., Bangalore - 64.

2. Centre for Scientific & IndustrialConsultancy (CSIC),IISc, Bangalore

3. Beckman CoulterNo. 38/1, 3rd Floor Manasa Bldg,4th & 5th Main, 8th Cross,Malleshwaram, Bangalore - 03Tel: 9900252639Email: [email protected]

4. Lead Instruments#20/4 New Bamboo Bazaar RoadBangalore - 560 051Tel: 080-25543954Email: [email protected]

5. Care Biomedical#137, Canara Bank Colony,Nagarbhavi Road, Bangalore - 560 072Tel: 9448129098Email: [email protected]

6. J. J. Biotek#36, 5th Cross, 9th MainK. N. Extension, YeshwantpurBangalore - 560 021Tel: 9448133739Email: [email protected]

7. Inkarp Instruments#29 "Adigiri", 1st Cross,Micro Layout, Mahalaxmipuram,Bangalore - 560 086Tel: 9844021067Email: [email protected]

8. Microphil (India)No. U24, 3rd Cross, Pipeline,Malleswaram, Bangalore - 560003Tel: 9628817363Email: [email protected]

9. Biogene India208, Second Floor, Magnum House IKarampura Commercial ComplexNew Delhi - 110015Tel: 011-25920047Email: [email protected]

10. Takara / ClonetechDSS Imagetech Pvt. Ltd.New DelhiTel: 09871130106Email: [email protected]

11. Laben#599/4, Shop No. 3,2nd Main Road, Prakash Nagar,Bangalore - 560 021Tel: 9845089463Email: [email protected]

12. OlympusDSS Imagetech Pvt. Ltd.245, Raheja Arcade,Second Floor, 1/1,Koramangala Industrial LayoutBangalore - 560095Tel: 9845294187Email: [email protected]

13. Becton Dickenson#9 & 10, First Main,Pampa Extn.Hebbal Kempapura,Bangalore - 560 024Tel: 9538999875Email: [email protected]

14. Imgenex IndiaE-5, Infocity, KIIT Post OfficeBhubaneshwar - 751 024Tel: (0674) 2743265Email: [email protected]

15. Millipore India Pvt. Ltd50A, 2nd Phase, Ring RoadPeenya, Bangalore - 560 058Tel: 080-3922 4000Email: [email protected]

16. SISCO Research Laboratories26, Navketan Ind., PremisesShanti Nagar, Mahakali Caves Road,Andheri (E), Mumbai - 93Tel: 022-426805800Email: [email protected]

With Best Compliments from:

Lab (India)Engineersnd599/4, # 3, 2 Main, Prakash Nagar, (Opp Navrang Restaurant, Dr. Rajkumar Road), Bangalore – 560 021.

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JJ Biotek Product Profile

SANYO – JAPAN152’C Deep Freezer, -86’C Deep Freezer, -40’C Deep Freezer, -20’C Deep Freezer, Pharmaceutical Refrigerators, Cold Chambers, Pharmaceutical Refrigerators with Freezer, Co2 Incubators, Incubators, Cooled Incubators, Plant growth chambers, Hot Air Oven, Dry Heat Sterilizers, Auto Clave, Blood Bank Refrigerators. Bio safety Cabinets and Data logging systems

QIAGEN – GERMANYGenomic DNA Preparation, Plasmid DNA Purification, DNA Cleanup and Normalization, RNA Sample Technologies, RNAi Solutions, PCR Technologies, Protein Technologies, Transfection Technologies, Automated Solutions, Real Time PCR, QIA Cube, QIAgility, QIAsymphony, EZI Advanced, QIAxtractor

N-BIOTEK – KOREALive Cell Engineering Station, DNA Vacuum Concentrators, Gas Blowing Station, IR Concentrators, Roller/Shakers, Incubator, Shaker Incubator, Refrigerated Incubator Shaker, Desktop Shaking Incubator, Hybridization Oven, BIO Work Station, PCR Work Station Bio-Safety Cabinet, Vortex Mixer, Dry Bath, Drying Oven, Hot Plate Stirrer

TECHNE – UKGradient PCR Machine, Non-Gradient PCR Machine, Hybridization Oven, Gelation Timer, Sample Concentrator, Biological Stirrer, Sample cooling, Dri-Blocks, Sample cooling, Thermo regulators

BIOGENICS – USAProgrammable Controlled Rate Freezers, Liquid Nitrogen Container, Cryostorage and Transport Instruments, Stage and Specimen Warmers, Portable Incubators, Cell Fusion

SYNGENE – UKOver 60,000 units of Gel Documentation System Installation all over the world. Fluorescence Gel Documentation System, Chemiluminescence and Fluorescence Gel Documentation System, Chemiluminescence Gel Documentation System, Proteomics and TLC Imaging System, 2D Imaging work station

HETTICH – GERMANYMicro Centrifuge, Table Top Micro Refrigerated Centrifuge, Table Top High Speed Refrigerated Centrifuge, Large Volume Refrigerated Centrifuge, Hematocrit Centrifuge, Automatic Cell Washing System.

JJ BIOTEK – INDIAOne stop and turnkey solution for lab set up for STEM CELLS, NANO TECHOLOGY and CRO labs from Industries experts.

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Please contact for Sales : Mr. Johnson + 91 98809 33838 and Mr.Prakash Prabhu + 91 94481 33739

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