Thermo ScientificDSQ™ II Series Single

Quadrupole GC/MS

c h r o m a t o g r a p h y

Part of Thermo Fisher Scientific

Redefining Productivity in the Workplace

Unmatched sensitivity and large dynamic range for demanding environmental analyses

Proven performance for difficult forensic matrices

Extend the range of sample analysis with the addition of solidsprobes for material characterization or unknown analysis

Productivity solutions available for immediate method implementation

Continuing over 30 years of GC/MS excellence, Thermo ScientificDSQ™ II single quadrupole GC/MS systems will change the way youthink about GC/MS analysis. These benchtop systems take tracelevel analysis into the future by providing innovation and perform-ance while ensuring reliable, accurate analytical results

Equipped with the new innovative DuraBrite™ XL source andDynaMax XR detection system, the DSQ II Series is able to detectsmaller amounts of compounds in difficult matrices, translating intoless prep time, smaller injections, less sample, more confidence –real productivity.

The Thermo Scientific DSQ II Series provides not just an analyticalinstrument, but a complete laboratory solution including high perform-ance innovative technology, industry-specific reporting software,method development, and customer support. Whether you are in theEnvironmental, Forensics, Toxicology, Chemical, Petrochemical,Pharmaceutical, or Food and Beverage industry, we offer the perfectpartner for innovation, performance, productivity, solutions, and results.

Less Noise = Better DataFor years, the enemy of optimum GC/MS detectivity has been noise.Background noise arises from multiple sources: sample matrix,column bleed, and source-generated noise (e.g. noise created byexcited neutrals). Sample cleanup and routine column maintenancecan help reduce two sources of noise. The third, neutral noise, ismore challenging. Many GC/MS systems address the issue by simplybackground subtracting or “thresholding” out the noise. However, bymasking this low level response, critical isotope ions may be lost orcompromised and available dynamic range reduced. This can limitboth sensitivity and quantitative capability.

Our scientists found a way to virtually eliminate source noisebefore it reaches the MS detector, by inserting a curved quadrupolepre-filter to provide a path that excited neutrals cannot navigate.They are effectively removed and can no longer add noise to thebaseline. By eliminating, rather than masking the noise, the DSQ IIGC/MS systems obtain improved detectivity without sacrificingdynamic range or spectral integrity.

Delivering Sensitivity and Reliability 24/7

Introducing the Thermo Scientific DSQ II SeriesDual Stage Quadrupole GC/MS

5, 20, 100, 500, 2000, 10000, 50000 pg

EI Full-Scan Linearity and Spectral Purity of Hexachlorobenzene

Linearity for 5 pg to 50 ng of HCB in full-scan mode with a linear fit of R2 = 0.9996.

noise reducedto ~2 ions/sec

Off-axis optics in the DSQ II provide a quantitative reduction in neutral noiseversus a straight-line quadrupole system.

DSQ II off-axis opticsOn-axis optics

Design innovations offer fundamental improvement in your analytical results

Confident Library Searches Regardless ofthe ConcentrationUnknowns are just that: unknown in composition and unknown inconcentration. The DSQ II’s extended dynamic range provides analyticalprecision at the low femtogram level as well as the high nanogramlevel. Your GC/MS must also provide spectral consistency over thesame dynamic range to produce confident library searching. DSQ IIsystems deliver this by combining the noise reduction of the curvedpre-filter with the robust performance of the DuraBrite XL ion source.

Rugged and Reliable EI Full-Scan PerformanceElectron Ionization (EI-MS) is the choice for most routine GC/MSanalyses. Whether an application requires the spectral characterizationand library matching of full-scan MS or demands the ultimate analytical sensitivity of Single Ion Monitoring (SIM), the system mustbe flexible to meet any challenge. It must also be rugged enough toperform the job, day in and day out.

Unmatched Positive and Negative ChemicalIonization CapabilitiesThe DSQ II shatters previous barriers to quantitative sensitivity in CI-SIM mode. Combining the innovative design of the ion opticswith the outstanding performance of the Thermo Scientific TRACEGC Ultra™ the DSQ II provides amazing analytical detectivity in theultra-low femtogram range.

Extra Sensitivity When You Need ItThe ability to detect trace amounts of compounds can mean lessprep time, smaller injections, less sample, more confidence – realproductivity. The new DuraBrite XL source improves ion transmissionin matrix for enhanced sensitivity, while protecting the analyzer fromcontamination. This maintains the system's unbeatable sensitivitylonger, and increases system uptime.

Fast Scanning at Greater than 11,000 amu/secThe DSQ II mass spectrometer offers the industry’s fastest scanspeed for single quadrupole systems. Faster scanning meets therequirements of fast chromatography. More data points define narrow GC peaks for precise and accurate quantitation.

Removable Ion VolumesComplete minor source maintenance without tedious source disassembly. Add the Vacuum Interlock to replace ion volumes without venting the system and begin analyzing samples in underthree minutes!

Sensitivity with rock-solid stability – replicate injections of 20 fg OFN over 24 hours. * Examples shown are raw data – no background subtraction, no baseline smoothing,and methane reagent gas.

NCI SIM: 1 fg OFNArea Count: 4,108Signal to Noise: 38 RMS

NCI SIM: 0.5 fg OFNArea Count: 2,168Signal to Noise: 27 RMS

NCI SIM: 0.25 fg OFNArea Count: 929Signal to Noise: 17 RMS

Making Precision G

C/MS A

ffordable

Easy to Use Advanced GC/MS Technology

Sequential SIM and Full-Scan Data AcquisitionSequential Full Scan/SIM capabilities allow you to combine the ultimate sensitivity of selected ion monitoring (SIM) for target compound analysis with the confidence of full-scan data for librarysearching and identification of unknowns, all in a single injection.

Removable Pre-filterThe pre-filter traps the “dirt”that can contaminate sensi-tive quadrupoles. Withsource side removal, simplyremove the pre-filter, cleanit and reinstall to restoreinstrument performance.Plus, with the DSQ II's solidmolybdenum rods that resistion burn, any contaminationthat may move past the prefilter will not build upand require replacement ofthe rod set.

TRACE GC Ultra – The Essential DSQ II PartnerIt really doesn’t matter how advanced the technology in the massspectrometer is if the GC cannot deliver the sample reliably androbustly. The process of GC/MS begins with the gas chromatograph.

Experience the performance of the industry-leading split/splitless,PTV, and innovative Cold-On-Column Large Volume injectors. TheThermo Scientific TRACE GC Ultra offers inlet technology to meetany sample requirement.

The GC can be configured with up to two traditional detectorsincluding FID, TCD, ECD, FPD, NPD, PDD and PID. The ThermoScientific Xcalibur™ data system controls each detector and simulta-neously acquires data from the two analog detectors and the DSQ IImass spectrometer.

Take care of your source maintenancewithout venting the system with theoptional Vacuum Interlock.

The removable pre-filter is easily

cleaned. Dirty samplesaren’t a problem.

High performing, robust, reliable, and affordable – maximum valuefor your GC/MS instrument investment

DSQ II GC/MS withTRACE GC Ultra

DSQ II GC/MS with FOCUS GC

TriPlus Autosampler

Thermo Scientific FOCUS GC – Powerful Performance in a Space-saving PackageThe FOCUS GC is designed for laboratories wanting the ultimate in GC/MS performancebut having limited space and funds. Equipped with a full-size GC oven, the FOCUS GC isequipped with our industry-leading split/splitless injector. Combined with the DSQ II, thissystem is a compact but powerful analyzer for environmental, petrochemical, forensics,and QA/QC applications.

Achieve Repeatable Carrier Gas Flows at the Touch of a ButtonThe TRACE GC Ultra and FOCUS GC virtually eliminate retention time shifts caused bycolumn-to-column variations. With the proprietary Column Evaluation feature on the GC, simply install the column and push the Column Eval button on the keypad. Theseinstruments perform automatic calibrations of the column to give precise and repeatableflows from column to column. This calibration ensures that your retention times will holdno matter how often you change the column, saving you the time and frustration of drifting retention times for your target compounds.

Thermo Scientific TriPlus™ Autosampler – Flexible Sampling SolutionsWith advanced robotic technology and independent three dimensional (XYZ) movement of the syringe holder, the TriPlus Autosampler provides flexibility in sample capacity for high lab productivity, while delivering unprecedented lab automation.• TriPlus AS for injection of liquid samples• TriPlus HS for automatic introduction of gases• TriPlus DUO includes both liquid and headspace sampling turrets• TriPlus SPME for automated solid phase extraction*• Easy “snap-on” interchangeability of 2 turrets• Double productivity with simultaneous operation of 2 different

Thermo Scientific GCs or GC-MS using 2 independent sampling methods*Sold under license from Supelco®

DSQ II Features

• Mass range of 1 – 1,050 amu• Scan speeds greater than 11,000 amu/s

(profile and centroid)• Curved pre-filter for noise reduction • DuraBrite XL source for enhanced robustness and sensitivity• ± 10 kV conversion dynode for better performance • Wide spectral/quantitative dynamic range• Removable ion volumes for EI and CI• Combination ion volume for automated switching

between EI and CI• Powerful Xcalibur data system• Closed Exit EI (CEI) Ion Volume for contaminated

environmental and food analyses

Analytical Versatility• EI mode for identifying unknowns – library search

information• PCI for molecular weight confirmation• NCI for ultimate sensitivity and selectivity• Simultaneous full-scan and SIM in a single analysis• PPINICI – hardware/software option with alternating

PCI/NCI scans for sample screening

Expanded Capabilities• Vacuum interlock• Liquid and headspace autosamplers• New TriPlus Autosampler for ultimate sampling flexibility• DEP/DIP solids probes• High-capacity split-flow pumping system• Two positions available for traditional GC detectors• Range of injection options• Productivity Solutions provide full method validation and

enable the operator to be productive within days of installation.

Providing Complete A

nalysis andReporting Capabilities Chemical Ionization for Molecular Weight

Confirmation and Enhanced SelectivityDetermining molecular weight is a must for today’s high throughputcombinatorial chemistry laboratory. Whether it is for natural products,drug discovery, or an organic synthesis operation this techniqueanswers the crucial question – Is it what I think it is?

In addition to molecular weight determinations, negative ionchemical ionization can provide enhanced selectivity, hence detectivity,of certain classes of compounds in complex matrices. This canimprove your ability to detect target compounds as well as quantitatethem at low levels in very dirty samples.

DSQ II makes the process of performing chemical ionizationsimple, routine, and robust.

Pulsed Positive Ion Negative Ion CI (PPINICI)Proprietary pulsed positive ion/negative ion chemical ionization(PPINICI) incorporates hardware and software to let you performboth PCI and NCI on alternating scans in the same injection.Xcalibur automatically filters the data for easy viewing, and havingboth positive and negative CI information enables more confidentmolecular weight analysis or sample screening.

Exchangeable Ion VolumesNo need to vent your system toperform a source swap. Simplyexchange your EI ion volume with the CI or EI/CI combo ion volume through the vacuum interlock (included with the CIoption), and continue your analysis – in under three minutes.

Continuing our tradition of providing the most inert ion sourcematerial, the newly developed closed EI (CEI) ion volume providesadditional stability and robustness for a number of applications. The CEI volume allows for an improved distribution of energy in thesource, while overall sensitivity is increased by a very tightly focusedbeam of ions guided by the lenses into the curved prefilter.

Digital Reagent Gas ControlDSQ II’s digital control stores the CI flow setting with each method –guaranteeing the same critical flow, day to day, week to week, operator to operator. No more tedious setup to manually adjust thereagent gas is required to reproduce previous work. The digital flowcontrol module is capable of automatically controlling a range ofcommonly used reagent gases, including ammonia, carbon dioxide,isobutane, and methane.

Solids ProbeProbe analysis allows rapid qualitative screening of bulk samples oranalysis of thermally labile compounds which degrade in the GCinlet. Interchangeable probes allow you to quickly switch betweenDirect-Exposure (DEP) or Direct-Insertion Probe (DIP), depending onthe sample.

Target Unknowns with Powerful Tools

Solids probe analysis of the flame retardant Decabromobiphenyl (MW 943),compared to a theoretical mass spectrum.

Probe Analysis of Decabromobiphenyl

Theoretical mass spectrum

DSQ II/Probedata

Simultaneous molecular weightconfirmation of Hexachloro-benzene in positive ion [M + H]+

and negative ion [M]-.

PPINICI Analysis of Hexachlorobenzene

PCI-TIC

NCI-TIC

PCI (M + 1 = 283)

NCI (M = 282)

Expand capabilities with the Positive and Negative CI optionand/or Solids Probe for non-GC sample introduction

EI, Closed EI, CI

Xcalibur is the most powerful data system available today, deliveringa unique combination of functionality, control, and ease of use. Thesoftware is designed to guide you through your daily tasks.Thispowerful simplicity, combined with the advanced features of theMicrosoft® Windows® operating system and Microsoft Office productivity tools provides an analytical platform second to none.

Xcalibur is organized around the Home Page. This starting pointdirects you to the six major functions of Xcalibur: Instrument Setup,Sequence Setup, Processing Setup, Qual Browser, Quan Browserand Library Browser. This design allows you to quickly and easilyaccess the area you need to work in without having to page throughadditional programs.

DSQ II GC/MS instruments generate library-searchable spectraregardless of matrix and concentration. This is a vital asset whendealing with complex unknown samples. Use commercial libraries,NIST, Wiley, Pfleger-Maurer-Weber, the Thermo Scientific PesticidesLibrary or build your own. Xcalibur allows simple exporting of spec-tra and has powerful editing tools to aid you in quickly generatingyour own user libraries. Xcalibur allows you to search multiplelibraries simultaneously. Thus you can easily match your unknownagainst multiple sources for confident identification.

Xcalibur provides complete control of the TRACE GC Ultra andFOCUS GC, mass spectrometer and optional liquid or headspaceautosamplers. Xcalibur contains a built-in audit trail to ensure

compliance with your laboratory’sSOP’s and Quality Programs.

Generating and producingreports from your data has neverbeen easier than with the XReportsreporting package in Xcalibur.XReports allows you to quicklyspecify each piece of information,mass trace, instrument setting,etc. to include in your report.Simple drop-down menus and“drag-and-drop” objects provide complete flexibility for your reporting needs.

Thermo Scientific Xcalibur – A Unified Platform forChromatography and Mass Spectrometry

One intuitive platform for GC/MS, LC/MS and Advanced MS instrumentsprovides confident control from method development to reporting

Xcalibur Unifies All the Technologies We Offer• Reduces the learning curve• Simplifies the addition of new technology platforms• Improvements are shared across all systems• Allows for customization through open source macro language

Integrates Layered Applications• EnviroLab™ Forms – Automated reporting forms package designed for the

regulated environmental laboratory• QuanLab™ Forms – Flexible general-purpose quantitation package ideal for routine

GC/MS applications• ToxLab™ Forms – Automated reporting forms package designed for

forensic/toxicology labs• ToxLab 2.0 – Intelligent sequencing and automated reporting forms designed for

drugs of abuse testing, therapeutic drug monitoring, environmental toxicology, andforensic applications

• Mass Frontier™ – Interpret mass spectral data with tools for fragments, structures,isotope patterns, spectral classification and more

• XDK – Open source macro programming interface for full customization of the data system

Xcalibur home page

The Thermo Scientific DSQ II GC/MS is ideal for GC/MS applicationsfrom research to routine. Thermo Scientific Lab Forms softwarepackages provide optimal productivity and complement routineapplications. Lab Forms packages offer standard features thatstreamline your workflows. Innovative new Method Forge providesan automated pathway to generating full scan methods. Wizardsand templates make programming daily batches straightforward,greatly reducing the time needed to queue samples and beginacquiring data.

Where the Lab Forms software packages truly redefine workflowsis with a revolutionary approach to data review and reporting.Through powerful Smart Reporting, data review and data reportingare dynamically linked, allowing for real-time changes to reportsbased on changes to the data. For example, if the Active Reportshows an out-of-range ion ratio, simply double-click the result to gostraight to that compound, for that sample. Review the integration –if it’s as simple as re-drawing the peak integration, then this changecan be made within Data Review. Return to the active report andthe new integration is immediately reflected – no reprocessing, no re-analyzing the data.

The Lab Forms packages include general purpose software,Thermo Scientific QuanLab Forms, ideal for GC/MS quantitationacross a wide range of applications. Thermo Scientific EnviroLab™

Forms provides reporting options that facilitate workflows for routineenvironmental GC/MS applications. Thermo Scientific ToxLab Formsincludes report styles and quality control settings that suit theneeds of forensic and toxicology laboratories.

QuanLab FormsQuanLab Forms provides a powerful productivity tool for quantitativeand semi-quantitative GC/MS experiments across a broad range ofapplications. QuanLab Forms provides an integrated, workflow-orientedapproach to GC/MS analysis and reporting. A wide selection ofstandard reports allows you to easily report data in a style that suits your lab’s needs. Generate reports in real time during sampleacquisition, or review and report data through the intuitive Data Review screen.

EnviroLab FormsToday’s environmental labs often must balance high productivity andthroughput with stringent quality control protocols. EnviroLab Formshas been designed to follow environmental laboratory workflows,from sample to result. EnviroLab Forms features a wide range ofreport options tailored to the needs of environmental laboratories.Because reviewing environmental data can be very time-consuming,Smart Reporting provides a revolutionary means of reviewing yourdata. Simply double-click out-of-range or questionable results to go straight to Data Review to review the sample. Any manual integrations are flagged for auditing purposes, and reports are ready to approve and print.

ToxLab FormsToxLab Forms is designed to offer an intuitive, work-flow orientedapproach to GC/MS acquisition, analysis, and reporting in the clinicalor forensic toxicology laboratory. ToxLab Forms streamlines the pathfrom unknown sample to known result in a manner that is secure,defensible and aligned with your lab’s specific needs. Enhancementsspecific to your toxicology lab’s needs are built into the system,including pre-developed standard report formats that simplify reporting of results. ToxLab Forms provides automated spectral librarysearching on non-target peaks and reports semi-quantitative resultsfor these unknown compounds.

Tailored Workflow

-Oriented

Solutions for Data A

nalysis

Powerful Software Packages Follow Laboratory Workflowsfor Maximum Productivity

Active Review

Method Forge

Data Review Calibration Curve

Thermo Scientific ToxLab 2.0ToxLab 2.0 software with true Intelligent Sequencing offers additionalcapabilities designed to take routine quantitative GC/MS confirmationsto the next level. ToxLab makes real-time decisions about samplequality and results based on criteria that you provide, and IntelligentSequencing takes the actions that you specify for each situation. For example, despite efforts to account for high concentrations basedon initial screen results, samples that exceed instrument carryoverlimits still come through the system. ToxLab can automatically inserta solvent blank following a high sample, thereby averting potentialcarryover issues and significantly reducing the need for manual re-injections or sample re-extractions. Failed calibration? ToxLab can abort the batch, saving time and money. Ion ratios out of range?ToxLab can be programmed to automatically reinject a sample,either following a blank injection or a simple reinjection. ToxLabfrees the GC/MS technologist or chemist from having to continuallymonitor batch progress and immediately improves your lab’s efficiencyand productivity. Reports can be generated either electronically orby print in real time during the batch, or the entire batch can bereviewed and printed after acquisition. For routine GC/MS quantita-tion, ToxLab with Intelligent Sequencing provides substantial timeand money savings through increased productivity and efficiency.

Easy Interpretation of Mass Spectral DataMass Frontier Software provides a suite of options that allows theMS operator to interpret mass spectral data. This software packageassists the operator in structural identification, isotope patterndetermination, and spectral classifications.

Customization of XcaliburThe Xcalibur Development Kit (XDK) is an open source macro programming code base that allows for full customization of thedata system. Any object oriented programming language can beused; there is no need to learn a proprietary programming language.The XDK provides information and example code for the creation of software by anyone to customize Xcalibur and reporting.

True Productivity and Easy Method Validation Productivity Solutions designed to complement the capabilities ofthe DSQ II provide pathways to complete method validation andintegration of the DSQ II into the laboratory. Productivity Solutionsinclude pre-developed and rigorously validated instrument and processing methods for targeted methodologies across a range ofapplications. A wide range of Thermo Scientific consumables arepre-selected for use in conjunction with these methods, and whereappropriate, customized libraries are included.

To further simplify integration, ProductivitySolutions include all informationrequired to operate the DSQ IIsuccessfully for the prescribedmethod. A printed SOP, How-ToGuide, and Quick reference guideare all available for reference,ensuring that a new DSQ II willbe productive within days.

Unsurpassed Intelligent Software Works For You

Comprehensive Solutions

for Your Laboratory

DSQ II GC/MS Configurations ELECTRON CHEMICAL HIGH COLUMNIONIZATION

IONIZATIONFLOW, LVIPROBE, PPINICI

70 L/s Turbo XBasic system for general EI applications, SIM or library confirmation in full-scan mode. Add GC detectors on the TRACE GC Ultra, and upgrade with autosamplers and selected MS options

250 L/s Turbo X XIncrease sensitivity and performance in EI. Add Chemical Ionization for molecular weight determi-nations or high sensitivity NCI-SIM quantitation.

200/200 L/s Split-flow Turbo X X XAdvance to the ultimate system with the split-flow pump which delivers best resolution and unmatched performance for PCI/NCI and ultra-trace levels.

DSQ II GC/MS – Performance and Reliability In Any Configuration

Available in various configurations, the DSQ II Series provides datamanagement and optional accessories to meet all analysis needs.

With the broadest range of chromatographic separation products in the industry, we helpmaximize efficiency and boost productivity by providing “one stop” sourcing for GC, GC/MS,specialized reporting systems, columns and consumables method development, and customer support.

From routine analysis to more demanding research applications, the full range of ThermoScientific instruments and accessories provide complete laboratory solutions to meet all needs.

DSQ II withFOCUS GC

DSQ II withTRACE GC Ultra TRACE GC Ultra

FOCUS GC

Enhanced Thermo Scientific TRACE™ GC columns, syringes and consumables, including injection port liners, ferrules, and septa, are designed to complement our innovative range of GC and GC/MS systems. This wide range of consumables and accessoriesis designed to offer application-focused solutions to customers in the petrochemical, food and beverage, forensic/toxicology, and environmental industries.

TRACE GC Columns Exhibit:• Low bleed• High operating temperatures• Low surface activity• Wide selectivity range

TRACE capillary GC columns are available with a wide polarityrange. High usable temperatures and low bleed are standard,increasing analytical sensitivity, reliability, and column lifetime.

Tailored Solutions for Your GC and GC/MS Analyses• TR-8270 – Semivolatiles • TR-1MS – PAHs, Hydrocarbons, and Sedatives• TR-35MS – Organochlorine and Triazine Pesticides, Drugs of Abuse• TR-5MS – Dioxins, Furans, Steroids, Drugs of Abuse• TR-1701 – Chlorinated Solvents • TR-WaxMS – Blood Alcohols• TR-V1 – Volatiles in Drinking Water• TR-FAME – Fatty Acid Methyl Esters

TRACE Columns and Consumables

Columns and Consumables

BR10003_E 06/08M

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Specifications, terms and pricing are subject to change.Not all products are available in all countries. Pleaseconsult your local sales representative for details.

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and technical support, to complete asset management and regulatory compliance

consulting. Improve your productivity and lower the cost of instrument ownership

through our product support services. Maximize uptime while eliminating the

uncontrollable cost of unplanned maintenance and repairs. When it’s time to

enhance your system, we also offer certified parts and a range of accessories and

consumables suited to your application.

To learn more about our products and comprehensive service offerings,

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