thermo scientific ltq velos dual-pressure linear ion trap · the instrument for the most demanding...
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Part of Thermo Fisher Scientific
World's fastest and most sensitive ion trap mass spectrometer
Thermo Scientific LTQ VelosDual-Pressure Linear Ion Trap
m a s s s p e c t r o m e t r y
The Instrument for the Most Demanding Sample Analyses
Thermo Scientific LTQ
VelosD
ual-Pressure Linear Ion Trap
2
The Thermo Scientific LTQ Velos is the latest evolution in the award winning LTQ family of linear ion trap mass spectrometers. Building on a tradition of innovation, the LTQ Velos™ provides the analyst with significantly enhanced performance for complex sample analyses.
The innovative dual-pressure ion trap and S-Lens ion optics combine to deliver increased ion transmission along with better trapping and fragmentation efficiency. The result is the fastest and most sensitive ion trap for all applications.
Continuing a commitment to providing researchers with the latest in high-performance mass spectrometers, the LTQ Velos is fully upgradeable with electron transfer dissociation (ETD) and Orbitrap™ technology to extend its capabilities even further.
• Unique dual-pressure linear ion trap technology provides superior scan speed and resolution
• Faster scanning and greater ion transmission improve identification of low-level analytes
• Simultaneous identification and quantification of even complex samples increases throughput
• Upgradable to accurate mass and ultra-high resolution Orbitrap technology
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Increased Performance Through Innovative Technology
Quantitation of Alprazolam in plasma yields an LOQ of 40 fg on column and demonstrates excellent linearity over 5 orders of magnitude
Innovative S-lens ion optics and dual-pressure linear ion trap make the LTQ Velos the fastest, most sensitive ion trap commercially available
The LTQ Velos excels at quantitative, as well as qualitative, analyses. With high-transmission ion optics and a fast scanning dual-pressure trap, the LTQ Velos is capable of simultaneously analyzing multiple low-level components at speeds compatiblewith U-HPLC systems.
The S-lens is a significant advance over high-pressure,skimmer-based ion optics designs. The superior iontransmission of the S-lens enables faster scan timesand increases overall instrument sensitivity.
Typically ion traps are operated at single pressure, a compromise between the optimum pressures for ion manipulation and ion detection. The revolutionary
LTQ Velos dual-pressure ion trap features a high-pres-sure cell that increases ion trapping efficiency to over90%. It also increases fragmentation efficiency, allowing fragmentation times to be cut to one third. The low-pressure cell increases scan speed by a factorof 2—to 33,300 u/s—while simultaneously improvingmass resolution by 0.1 u FWHM.
The LTQ Velos merges all these innovations and moreinto an ion trap mass spectrometer with unrivaled performance, including ultra-fast 10 Hz acquisition rate and low femtogram sensitivity.
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Alprazolam
R = 0.997
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HESI IISource Ion Transfer
TubeIon Trap
S-Lens
Octopole
HPTHPTHigh-
PressureCell
Low-Pressure
Cell
Detector1
Detector2
Thermo Scientific LTQ
VelosD
ual-Pressure Linear Ion Trap
4
Unprecedented Performance Generates GreaterInsight into Complex Samples
• Identify low-abundance proteins to extend information about cellular processes
• Determine protein expression changes with faster-scanning PQD for more quantifiable peptides
• Characterize the most complex PTMs using multiple dissociation techniques and MSn capabilities
• Analyze intact proteins and perform N-terminal sequencing with ETD
• Annotate results with information from public databases such as GO to illuminate biological context
LTQ Velos doubles the number of proteins and unique peptides identified by a 60-minute, data-dependent LC-MS/MS analysis of a 20 ng digest of C. elegans
Data-dependent acquisition by the LTQ Velos at 7 Hz - more than 2X the rate of the LTQ XL - producing high-quality MS/MS data
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0Proteins Unique Peptides
+152%
+123%
LTQ XL
LTQ Velos
LTQ Velos linear ion trap identified more peptides and proteins in a 60 min LC/MS run than an LTQ XL did in a 180 min run (1 μg digest of C. elegans)
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Routine analysis of complex proteomics samples is often confounded by the inability to adequately detect low-abundance proteins, by the large number of post-translational modifications (PTMs) possible, and because of the dynamic nature of the proteome. Ion trap-based mass spectrometry, however, has demonstrated a superior ability to rapidly identify and characterize low-abundance proteins, and determine their relative expression levels. It hasbecome an essential tool in proteomics labs.
The LTQ Velos enables a new level of proteomics sample interrogation, made possible by improvements in acquisition rates, MSn sensitivity, and selective accumulation of low-abundance ions. Predictive Automatic Gain Control (P-AGC) ensures that while the scan speed is increased, sensitivity is not compromised. Increased scan speed on the LTQ Velosalso facilitates ultra-fast LC separation for high-throughput analysis.
40 10060 80 120 160140Time (min)
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442.37
706.55
451.01
676.47
213.24392.04
244.23185.28407.92 576.52331.40 359.28 611.03 641.52479.32326.39277.34 804.65554.31 771.63
830.75
2 0 0 3 0 0 4 0 0 5 0 0 6 0 0 7 0 0 8 0 0 9 0 0 1 0 0 0 1 1 0 0m /z
9 2 2 .7 1
3 7 3 .3 5 6 8 3 .5 56 3 9 .6 45 8 3 .8 05 3 2 .3 9
6 1 1 .0 62 4 4 .2 4
1 0 5 1 .7 27 6 9 .4 4
5 2 6 .4 9 8 9 8 .5 94 6 1 .9 52 9 9 .2 67 5 4 .5 63 9 8 .3 72 2 7 .2 4 7 8 8 .8 4 9 3 8 .6 55 1 6 .3 43 5 6 .3 9 8 8 0 .7 34 1 5 .5 2 1 0 2 2 .8 7 1 0 7 9 .7 0
2 0 0 3 0 0 4 0 0 5 0 0 6 0 0 7 0 0 8 0 0 9 0 0 1 0 0 0m /z
4 7 3 .5 0
5 2 1 .0 6
3 8 3 .3 5
7 6 2 .5 4
4 5 4 .4 1
5 7 0 .6 76 0 6 .2 0
4 0 2 .4 96 4 9 .3 7
3 0 3 .4 06 7 9 .5 6
2 0 1 .2 22 3 2 .1 6
8 4 9 .6 22 7 8 .3 91 7 3 .1 9 3 5 5 .6 17 1 9 .5 2 7 7 8 .6 4 8 7 0 .6 6
9 6 9 .7 31 0 4 0 .7 49 1 3 .9 4
2 0 0 2 5 0 3 0 0 3 5 0 4 0 0 4 5 0 5 0 0 5 5 0 6 0 0 6 5 0 7 0 0 7 5 0 8 0 0 8 5 0 9 0 0m /z
6 9 7 .5 3
5 1 1 .2 4
7 9 6 .6 3
5 6 9 .4 02 4 4 .2 8
3 4 3 .4 0
6 5 5 .7 04 8 2 .4 3
3 1 9 .4 23 9 9 .0 8
4 7 1 .3 85 5 8 .4 2 7 2 1 .5 3
5 8 0 .6 12 1 6 .2 3 4 1 9 .8 3 7 7 8 .6 02 9 8 .3 8
3 9 0 .2 0 5 9 1 .5 74 5 8 .7 0 8 6 5 .5 97 5 5 .6 58 2 5 .7 0 9 0 9 .8 0
2 0 0 3 0 0 4 0 0 5 0 0 6 0 0 7 0 0 8 0 0 9 0 0 1 0 0 0 1 1 0 0 1 2 0 0 1 3 0 0 1 4 0 0m /z
9 3 3 .7 0
5 0 6 .3 5
7 1 1 .4 5 1 1 6 1 .7 4
7 0 1 .5 8 1 3 1 0 .7 81 0 4 8 .6 9
2 7 8 .3 24 8 9 .3 5 6 5 6 .3 03 9 1 .3 8 5 9 1 .4 1 8 3 1 .6 47 3 2 .6 3 1 2 9 2 .8 69 5 9 .6 83 7 3 .3 7 4 6 0 .3 6 9 1 5 .7 02 6 0 .3 7 1 1 4 5 .7 78 1 3 .6 3 1 2 7 5 .8 8 1 3 3 8 .6 9
Five consecutive MS/MS spectra in 0.7 s
Facilitating our Understanding of Systems Biology
Ultimate Resolution and Sensitivity for ProteinCharacterization
2865.82
2812.91 2920.82
2978.092764.82
3037.55
2712.64
3102.73
2665.00
3163.912621.91
3231.182490.183305.45
z = 53
z = 52
z = 51
z = 50
z = 49
z = 48
z = 54
z = 55
z = 56
z = 57
z = 58z = 47
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2868.91
2815.91
2862.91 2872.00
2809.912818.82
2874.64
2821.36
2824.362859.822807.00
2829.82
2892.272790.36
z = 542812.91
z = 532865.82
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The LTQ Velos offers resolving power rivaling that ofmany commercial Q-TOF mass spectrometers to aid in top-down characterization of proteins. Additional
improvements in the efficiency of detection in extendedmass ranges provide unmatched sensitivity for largemacromolecules, such as intact antibodies.
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941.93
997.18847.85
770.93
737.43
1059.44
706.76
1130.02
678.511210.61
1303.61652.42
628.34 1412.20 1540.451694.29
605.92585.67
z = 16
z= 15
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z = 12 z = 11z = 10
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Low-Resolution Normal Scan
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Experimental
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LTQ Velos Ultra ZoomScan analy-sis of intact equine myoglobin(~17 kDa), 15+, demonstrates isotopic resolution greater than 25,000 FWHM
ESI-MS envelope from LTQ Velosanalysis of intact IgG (152 kDa),obtained by averaging spectraover a 1-minute wide LC peak
Thermo Scientific LTQ
VelosD
ual-Pressure Linear Ion Trap
6
The characterization of drug metabolites plays a pivotalrole in pharmaceutical discovery and development.Parent drug and metabolites need to be identified in avariety of biological matrices over a wide range of con-centrations for both in vivo and in vitro studies. Whenused together, Thermo Scientific MetWorks and MassFrontier software and the LTQ Velos create a robust,sensitive, integrated workflow for identification, charac-terization, and quantitation of drugs and drug metabo-lites in biological matrices.
The rapid scan speed of the LTQ Velos allows simultane-ous qualitative and quantitative metabolite analysis tobe carried out easily for both in vivo and in vitro studies.The faster scan rates of the LTQ Velos allow more MSn
experiments to be carried out in shorter chromatograph-ic runs. This, along with improved sensitivity, results inmore high-quality ion trees for qualitative metaboliteanalysis and characterization. The use of DataDependant™ acquisition coupled with DynamicExclusion™ provides a methodology for the analysis of both predicted and unexpected metabolites.Multiple dissociation methods provide the analyst with more approaches to structural elucidation.
• MetWorks™ software uses MSn spectral trees for unambiguous structural elucidation; e.g.differentiation of structural isomers and identification of the site(s) of biotransformation.
• MetWorks metabolite identification software integrates acquisition, processing and reporting capabilities.
• Mass Frontier™ facilitates the spectral interpretation by predicting fragmentation pathways and automated assignments of structures to spectral trees.
Two ion trees of two isomeric mono-hydroxylated metabolites
Processing of metabolism data is supported with both MetWorksand Mass Frontier software
Modifications View from MetWorks software
Maropitant
Hydroxylation
Demethylation
Full ScanMass Spectra
Product IonMass Spectra
Hydroxylation of Maropoitantat retention time 3.46 min
Hydroxylation of Maropoitantat retention time 4.53 min
Comprehensive Compound Identification and Quantitation of Complex Samples
3.46 min 4.53 min
MS3
MS4
MS2
MS
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High-Quality Data for Metabolomics Analyses
Feature segregation and identification are key components ofall metabolomics workflows. While software packages suchas SIEVE discriminate components of interest as potentialmarkers of a condition, feature identification relies on thequality of the data generated by the mass spectrometer. The LTQ Velos greatly extends the performance of the groundbreaking, robust and sensitive LTQ linear ion trap, producing more high-quality MSn data from smaller amountsof sample. Mass Frontier is a pivotal component of themetabolomics workflow. It melds the acquisition speed, sensitivity and information-rich MSn spectra generated by the new LTQ Velos with the power of spectral trees andlibrary or database searching. The combination of mass spectrometric performance, advanced feature detection, powerful metabolite identification and intuitive/integrated software solutions simplifies problem solving.
• Routinely Quantify Sets of Identified Metabolites in Large Sample Populations
Quantify
• Endogenous Metabolites
• Database Search and Mass Frontier
Identify
• Genedata Expressionist
• SIEVE, SIMCA-P (Umetrics)
• Differentially Expressed Small Molecules
Discover
• Acquire nominal-mass or accurate-mass LC/MS
Acquire
Mass Frontier software provides tools for automated MSn spectral tree deconvolution, fragmentation prediction and spectral annotation
BR63051_E 05/09S
Thermo Fisher Scientific,San Jose, CA USA is ISO Certified.
©2009 Thermo Fisher Scientific Inc. All rights reserved.SEQUEST is a registered trademark of the University ofWashington. Mass Frontier is a trademark of HighChemLtd. Microsoft, Microsoft Windows, and Microsoft Officeare trademarks of Microsoft Corporation. Intel is atrademark of Intel Corporation. All other trademarks are the property of Thermo Fisher Scientific Inc. and itssubsidiaries.
Specifications, terms and pricing are subject to change.Not all products are available in all countries. Pleaseconsult your local sales representative for details.
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Specifications
Software FeaturesData System• Thermo Scientific Xcalibur processing and instrument
control software• Microsoft® Office XP software package• Microsoft Windows® XP operating system• High-performance PC with Intel® microprocessor • High-resolution LCD color monitor
Scan Functions• Full-scan feature provides full-scan mass spectra for
sensitive analyses and produces spectra for rapidscreening of unknown compounds
• Selected ion monitoring (SIM) monitors selected ionsfor target compound analysis
• Full-scan MS/MS produces full-scan product ionspectra selected reaction monitoring (SRM) for a traditional LC-MS/MS quantitative analytical experiment
• MSn for multi-stage MS experiments to probe thestructure of ions
• ZoomScan™, a high-resolution, full-range scan toresolve isotopic envelopes which is often used forcharge state determination of peptides and ionizationstate determination of organometallics
• Ultra ZoomScan for ultimate resolution • TurboScan™, an ultra-fast scan to improve
signal-to-noise and sampling rate
Exclusive Technologies• Unique, patented Automatic Gain Control (AGC)
ensures that the ion trap is always filled with theoptimum number of ions for any scan type
• Dynamic Exclusion™ allows acquisition of MS/MS andMSn spectra from lower intensity ion species
• WideBand Activation™ generates more structurallyinformative spectras
• Normalized Collision Energy™ provide reproducibledata from instrument to instrument
System SpecificationsMS/MS SensitivityElectrospray Ionization (ESI) –2 μL of a 50 fg/μLsolution of reserpine (100 femtograms total) injected at a flow of 500 μL/min will produce a minimum signal-to-noise ratio of 100:1, for the transition of theisolated protonated molecular ion at m/z 609 to thelargest two product ions, m/z 397 and m/z 448, whenthe mass spectrometer is operated at unit resolution inthe full-scan MS/MS mode, scanning the product ionspectrum from m/z 165 – 615.
Atmospheric Pressure Chemical Ionization (APCI) – 2 μLof a 50 fg/μL solution of reserpine (100 femtogramstotal) injected at a flow of 500 μL/min will produce a minimum signal-to noise ratio of 100:1, for the transition of the isolated protonated molecular ion atm/z 609 to the largest two product ions, 397 and 448,when the mass spectrometer is operated at unit resolution in the full-scan MS/MS mode, scanning the product ion spectrum from m/z 165 – 615.
Installation RequirementsPower• One 230 Vac ±10.0%, 15 Amps, 50/60 Hz, single
phase, with earth ground dedicated to the instrument• 120 or 230 Vac single phase, with earth ground for
the data system
Gas• One high-purity (99% pure, flow rate 15 L/min)
nitrogen gas supply for the API source • One ultra-high-purity helium gas supply (99.998%
pure) with less than 1 ppm each of water, oxygen,and total hydrocarbons for the mass analyzer
Environment• System averages 2300 W (8000 BTU/h) output when
considering air conditioning needs • Operating environment must be 15-27 °C (59-80 °F)
and relative humidity must be 40-80% with no condensation
• Optimum operating temperature is 18-21 °C (65-70 °F)
Dimensions/Weight• MS: 56 cm ⋅ 79 cm ⋅ 59 cm (h ⋅ w ⋅ d)• MS: ~120 kg• Roughing pumps: 38.6 kg
Performance SpecificationsMass Range• m/z 15 – 200• m/z 50 – 2000• m/z 200 – 4000
Resolution• 0.05 FWHM (full width half maximum)
with Ultra ZoomScan
Scan Power• MSn, for n = 1 through 10
Analog Inputs• One (1) analog Input (0-1 V)• One (1) analog Input (0-10 V)